AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1403970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1403970 MtrunA17_Chr5g0398651 93.966 116 7 0 1 116 1 116 9.08e-77 222
Msa1403970 MtrunA17_Chr4g0056791 62.385 109 36 1 5 113 41 144 6.96e-40 130
Msa1403970 MtrunA17_Chr2g0320881 53.125 96 45 0 20 115 51 146 7.09e-27 98.6
Msa1403970 MtrunA17_Chr4g0027171 50.562 89 44 0 24 112 67 155 6.46e-26 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1403970 AT2G27830.1 52.128 94 45 0 19 112 61 154 1.09e-30 108

Find 70 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGGCAACTAAATATAATT+TGG 0.142333 tig0022985:-26251 None:intergenic
AAGATATTATTCACACTTAT+TGG 0.154119 tig0022985:+25952 Msa1403970:three_prime_UTR
ATCCAAACTCTCTTTGTCTT+TGG 0.236659 tig0022985:+25268 Msa1403970:five_prime_UTR
TCTCTTTGTCTTTGGCTTCA+TGG 0.240774 tig0022985:+25276 Msa1403970:exon
ACATCAAAACCTCACTGTTT+AGG 0.276948 tig0022985:+25016 Msa1403970:five_prime_UTR
AATCACTTTAGCATTAATTA+TGG 0.277764 tig0022985:-25982 None:intergenic
CTCTTTGTCTTTGGCTTCAT+GGG 0.284185 tig0022985:+25277 Msa1403970:exon
CAGGTCACTGCTGGAAAATT+CGG 0.287630 tig0022985:+25060 Msa1403970:five_prime_UTR
AATGAGTGAAGGAAGCTTCT+AGG 0.300519 tig0022985:-25147 None:intergenic
CATCAAAACCTCACTGTTTA+GGG 0.313886 tig0022985:+25017 Msa1403970:five_prime_UTR
AGCCAAAGACAAAGAGAGTT+TGG 0.330320 tig0022985:-25270 None:intergenic
TATCCTTTACATGTTGCATA+AGG 0.355932 tig0022985:-26270 None:intergenic
ATGTATCTTGTGAAAGGTTA+AGG 0.390025 tig0022985:+26064 Msa1403970:three_prime_UTR
AGGGCAACTAAATATAATTT+GGG 0.412005 tig0022985:-26250 None:intergenic
ATTATTTGTCAGGTCACTGC+TGG 0.415487 tig0022985:+25051 Msa1403970:five_prime_UTR
TGCATTGATGACTTATGATA+AGG 0.419305 tig0022985:+25471 Msa1403970:CDS
AACGGAAATTTGTCAGTTTG+AGG 0.425120 tig0022985:-25316 None:intergenic
TAGTGAGAAATTGAAGAACT+TGG 0.435583 tig0022985:+25839 Msa1403970:CDS
TGAGTAGTATGAGGTAGTTT+AGG 0.435990 tig0022985:+26224 Msa1403970:three_prime_UTR
TAATTACTTTCAAGCGTGAA+TGG 0.437696 tig0022985:-25553 None:intergenic
TGATGAGGCTATTATTTGTC+AGG 0.438001 tig0022985:+25041 Msa1403970:five_prime_UTR
AGAGTTTGGATTTCAGTTTA+CGG 0.441806 tig0022985:-25256 None:intergenic
ATGAAATGGTGCAGGTTTGA+AGG 0.447179 tig0022985:+25815 Msa1403970:intron
ATCCTTTACATGTTGCATAA+GGG 0.449046 tig0022985:-26269 None:intergenic
GGATCGGTGTCCTTCAATAA+TGG 0.452970 tig0022985:-25505 None:intergenic
GTGACTGATTGAATCTCAAT+TGG 0.460220 tig0022985:+26011 Msa1403970:three_prime_UTR
AAGTGAAGTTGGTAGCGGTT+TGG 0.461676 tig0022985:-25526 None:intergenic
TGCCCTTATGCAACATGTAA+AGG 0.473630 tig0022985:+26267 Msa1403970:three_prime_UTR
TGAACTTCCAAGGCAGCATA+TGG 0.474963 tig0022985:+25903 Msa1403970:exon
AGTGAGAAATTGAAGAACTT+GGG 0.475992 tig0022985:+25840 Msa1403970:CDS
AAGGGTGAAATTTGAGTTTG+TGG 0.476387 tig0022985:-25177 None:intergenic
TGAATGGAGAGAAGTGAAGT+TGG 0.482669 tig0022985:-25537 None:intergenic
TTGGAAGTTCAGATTGAAGT+AGG 0.484151 tig0022985:-25891 None:intergenic
AATTAATGCTAAAGTGATTG+AGG 0.488321 tig0022985:+25986 Msa1403970:three_prime_UTR
GAAGAGAAGGCCATTATTGA+AGG 0.491115 tig0022985:+25495 Msa1403970:CDS
AGGTCACTGCTGGAAAATTC+GGG 0.491361 tig0022985:+25061 Msa1403970:five_prime_UTR
ATATTTCGATTTGTAGAGGT+TGG 0.493266 tig0022985:-25105 None:intergenic
AGGGTGAAATTTGAGTTTGT+GGG 0.494506 tig0022985:-25176 None:intergenic
AATTGAAGAACTTGGGGTCA+AGG 0.499762 tig0022985:+25847 Msa1403970:CDS
TCAATTGCCATATGCTGCCT+TGG 0.502902 tig0022985:-25910 None:intergenic
TGGTCTAAGAGGAGAGTCAT+TGG 0.503838 tig0022985:-25353 None:intergenic
TGTTGTTGTTGTTACGAGAA+AGG 0.503927 tig0022985:-25219 None:intergenic
AAATTTATGAATCGTCAAGC+TGG 0.516087 tig0022985:+26192 Msa1403970:three_prime_UTR
AATCTTATGTATCTTGTGAA+AGG 0.518070 tig0022985:+26058 Msa1403970:three_prime_UTR
AGAAGTTTCGATGGTCTAAG+AGG 0.521243 tig0022985:-25364 None:intergenic
GAGAGAAGTGAAGTTGGTAG+CGG 0.523441 tig0022985:-25531 None:intergenic
CGAAGCTATTGAGTAGTATG+AGG 0.524961 tig0022985:+26215 Msa1403970:three_prime_UTR
TTTGTCTTTGGCTTCATGGG+TGG 0.526303 tig0022985:+25280 Msa1403970:exon
TCAAACTGACAAATTTCCGT+TGG 0.528724 tig0022985:+25318 Msa1403970:CDS
GTCAGTTTGAGGAGTAGAAA+CGG 0.529246 tig0022985:-25305 None:intergenic
GTGTCCATCGAGAACACGTT+GGG 0.537604 tig0022985:+25394 Msa1403970:CDS
CAAATTTCCGTTGGATACAA+CGG 0.549881 tig0022985:+25327 Msa1403970:CDS
TTGGTTTCCGTTGTATCCAA+CGG 0.551596 tig0022985:-25334 None:intergenic
TACCGTTGGCGAATTAGTGA+AGG 0.567706 tig0022985:+25447 Msa1403970:CDS
GGGTGAAATTTGAGTTTGTG+GGG 0.570497 tig0022985:-25175 None:intergenic
ACTTATATTTCGATTTGTAG+AGG 0.579774 tig0022985:-25109 None:intergenic
GTGAGAAATTGAAGAACTTG+GGG 0.585031 tig0022985:+25841 Msa1403970:CDS
CTTATGATAAGGAGAAGAGA+AGG 0.588206 tig0022985:+25482 Msa1403970:CDS
AAGTTGGTAGCGGTTTGGAT+CGG 0.590772 tig0022985:-25521 None:intergenic
CTCACTGTTTAGGGATGATG+AGG 0.592643 tig0022985:+25026 Msa1403970:five_prime_UTR
ATGTCGCCTGAAAATACCGT+TGG 0.599343 tig0022985:+25433 Msa1403970:CDS
TGCACCCAACGTGTTCTCGA+TGG 0.600223 tig0022985:-25398 None:intergenic
TACTTCAATCTGAACTTCCA+AGG 0.601898 tig0022985:+25893 Msa1403970:exon
TGTCCCTCAAAAGTGTTGAG+TGG 0.609379 tig0022985:-26034 None:intergenic
GGTGAAATTTGAGTTTGTGG+GGG 0.618265 tig0022985:-25174 None:intergenic
ACGTTGAGTAGAAGTTTCGA+TGG 0.635634 tig0022985:-25373 None:intergenic
TGTTGTTGTTACGAGAAAGG+TGG 0.636326 tig0022985:-25216 None:intergenic
AACCTTCACTAATTCGCCAA+CGG 0.655659 tig0022985:-25449 None:intergenic
CGTGTCCATCGAGAACACGT+TGG 0.692755 tig0022985:+25393 Msa1403970:CDS
CTCATCATCCCTAAACAGTG+AGG 0.694635 tig0022985:-25025 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGATATAAATTATGAAAAAA+GGG - tig0022985:25603-25622 None:intergenic 15.0%
!! AGGATATAAATTATGAAAAA+AGG - tig0022985:25604-25623 None:intergenic 15.0%
!! AAAAATGATATGAAAAAACA+AGG - tig0022985:25624-25643 None:intergenic 15.0%
!! AAGTAAAAAATAATACTGTT+TGG + tig0022985:26101-26120 Msa1403970:three_prime_UTR 15.0%
!! GGATATAAATTATGAAAAAA+GGG - tig0022985:25603-25622 None:intergenic 15.0%
!! AGGATATAAATTATGAAAAA+AGG - tig0022985:25604-25623 None:intergenic 15.0%
!! AAAAATGATATGAAAAAACA+AGG - tig0022985:25624-25643 None:intergenic 15.0%
!! AAGTAAAAAATAATACTGTT+TGG + tig0022985:26101-26120 Msa1403970:three_prime_UTR 15.0%
!! AAGATATTATTCACACTTAT+TGG + tig0022985:25952-25971 Msa1403970:three_prime_UTR 20.0%
!! AATCACTTTAGCATTAATTA+TGG - tig0022985:25985-26004 None:intergenic 20.0%
!! AAGATATTATTCACACTTAT+TGG + tig0022985:25952-25971 Msa1403970:three_prime_UTR 20.0%
!! AATCACTTTAGCATTAATTA+TGG - tig0022985:25985-26004 None:intergenic 20.0%
!! ACTTATATTTCGATTTGTAG+AGG - tig0022985:25112-25131 None:intergenic 25.0%
!!! GGTTTTTTTCAAAAGTGAAA+GGG - tig0022985:25198-25217 None:intergenic 25.0%
!!! TGGTTTTTTTCAAAAGTGAA+AGG - tig0022985:25199-25218 None:intergenic 25.0%
! AATTAATGCTAAAGTGATTG+AGG + tig0022985:25986-26005 Msa1403970:three_prime_UTR 25.0%
! AATCTTATGTATCTTGTGAA+AGG + tig0022985:26058-26077 Msa1403970:three_prime_UTR 25.0%
! AGGGCAACTAAATATAATTT+GGG - tig0022985:26253-26272 None:intergenic 25.0%
! AAGGGCAACTAAATATAATT+TGG - tig0022985:26254-26273 None:intergenic 25.0%
!! ACTTATATTTCGATTTGTAG+AGG - tig0022985:25112-25131 None:intergenic 25.0%
!!! GGTTTTTTTCAAAAGTGAAA+GGG - tig0022985:25198-25217 None:intergenic 25.0%
!!! TGGTTTTTTTCAAAAGTGAA+AGG - tig0022985:25199-25218 None:intergenic 25.0%
! AATTAATGCTAAAGTGATTG+AGG + tig0022985:25986-26005 Msa1403970:three_prime_UTR 25.0%
! AATCTTATGTATCTTGTGAA+AGG + tig0022985:26058-26077 Msa1403970:three_prime_UTR 25.0%
! AGGGCAACTAAATATAATTT+GGG - tig0022985:26253-26272 None:intergenic 25.0%
! AAGGGCAACTAAATATAATT+TGG - tig0022985:26254-26273 None:intergenic 25.0%
! ATATTTCGATTTGTAGAGGT+TGG - tig0022985:25108-25127 None:intergenic 30.0%
AGAGTTTGGATTTCAGTTTA+CGG - tig0022985:25259-25278 None:intergenic 30.0%
! TGCATTGATGACTTATGATA+AGG + tig0022985:25471-25490 Msa1403970:CDS 30.0%
TAATTACTTTCAAGCGTGAA+TGG - tig0022985:25556-25575 None:intergenic 30.0%
ATGAAAAAAGGGAACATTGA+AGG - tig0022985:25592-25611 None:intergenic 30.0%
TATACACGATGCATATGATA+AGG - tig0022985:25756-25775 None:intergenic 30.0%
TAATCAACCACCAAATGAAA+TGG + tig0022985:25801-25820 Msa1403970:intron 30.0%
TAGTGAGAAATTGAAGAACT+TGG + tig0022985:25839-25858 Msa1403970:CDS 30.0%
AGTGAGAAATTGAAGAACTT+GGG + tig0022985:25840-25859 Msa1403970:CDS 30.0%
ATGTATCTTGTGAAAGGTTA+AGG + tig0022985:26064-26083 Msa1403970:three_prime_UTR 30.0%
! AATCTAGCTGCATGTATTTT+TGG + tig0022985:26133-26152 Msa1403970:three_prime_UTR 30.0%
AAATTTATGAATCGTCAAGC+TGG + tig0022985:26192-26211 Msa1403970:three_prime_UTR 30.0%
ATCCTTTACATGTTGCATAA+GGG - tig0022985:26272-26291 None:intergenic 30.0%
TATCCTTTACATGTTGCATA+AGG - tig0022985:26273-26292 None:intergenic 30.0%
! ATATTTCGATTTGTAGAGGT+TGG - tig0022985:25108-25127 None:intergenic 30.0%
AGAGTTTGGATTTCAGTTTA+CGG - tig0022985:25259-25278 None:intergenic 30.0%
! TGCATTGATGACTTATGATA+AGG + tig0022985:25471-25490 Msa1403970:CDS 30.0%
TAATTACTTTCAAGCGTGAA+TGG - tig0022985:25556-25575 None:intergenic 30.0%
ATGAAAAAAGGGAACATTGA+AGG - tig0022985:25592-25611 None:intergenic 30.0%
TATACACGATGCATATGATA+AGG - tig0022985:25756-25775 None:intergenic 30.0%
TAATCAACCACCAAATGAAA+TGG + tig0022985:25801-25820 Msa1403970:intron 30.0%
TAGTGAGAAATTGAAGAACT+TGG + tig0022985:25839-25858 Msa1403970:CDS 30.0%
AGTGAGAAATTGAAGAACTT+GGG + tig0022985:25840-25859 Msa1403970:CDS 30.0%
ATGTATCTTGTGAAAGGTTA+AGG + tig0022985:26064-26083 Msa1403970:three_prime_UTR 30.0%
! AATCTAGCTGCATGTATTTT+TGG + tig0022985:26133-26152 Msa1403970:three_prime_UTR 30.0%
AAATTTATGAATCGTCAAGC+TGG + tig0022985:26192-26211 Msa1403970:three_prime_UTR 30.0%
ATCCTTTACATGTTGCATAA+GGG - tig0022985:26272-26291 None:intergenic 30.0%
TATCCTTTACATGTTGCATA+AGG - tig0022985:26273-26292 None:intergenic 30.0%
!! AGGTTTTGATGTTCATGGAT+TGG - tig0022985:25008-25027 None:intergenic 35.0%
ACATCAAAACCTCACTGTTT+AGG + tig0022985:25016-25035 Msa1403970:five_prime_UTR 35.0%
CATCAAAACCTCACTGTTTA+GGG + tig0022985:25017-25036 Msa1403970:five_prime_UTR 35.0%
TGATGAGGCTATTATTTGTC+AGG + tig0022985:25041-25060 Msa1403970:five_prime_UTR 35.0%
! AGGGTGAAATTTGAGTTTGT+GGG - tig0022985:25179-25198 None:intergenic 35.0%
! AAGGGTGAAATTTGAGTTTG+TGG - tig0022985:25180-25199 None:intergenic 35.0%
! TGTTGTTGTTGTTACGAGAA+AGG - tig0022985:25222-25241 None:intergenic 35.0%
ATCCAAACTCTCTTTGTCTT+TGG + tig0022985:25268-25287 Msa1403970:five_prime_UTR 35.0%
AACGGAAATTTGTCAGTTTG+AGG - tig0022985:25319-25338 None:intergenic 35.0%
TCAAACTGACAAATTTCCGT+TGG + tig0022985:25318-25337 Msa1403970:CDS 35.0%
CAAATTTCCGTTGGATACAA+CGG + tig0022985:25327-25346 Msa1403970:CDS 35.0%
CTTATGATAAGGAGAAGAGA+AGG + tig0022985:25482-25501 Msa1403970:CDS 35.0%
AAGGGAACATTGAAGGATTA+AGG - tig0022985:25585-25604 None:intergenic 35.0%
! CATTTGGTGGTTGATTAACA+TGG - tig0022985:25798-25817 None:intergenic 35.0%
GTGAGAAATTGAAGAACTTG+GGG + tig0022985:25841-25860 Msa1403970:CDS 35.0%
TTGGAAGTTCAGATTGAAGT+AGG - tig0022985:25894-25913 None:intergenic 35.0%
TACTTCAATCTGAACTTCCA+AGG + tig0022985:25893-25912 Msa1403970:exon 35.0%
GTGACTGATTGAATCTCAAT+TGG + tig0022985:26011-26030 Msa1403970:three_prime_UTR 35.0%
TGAGTAGTATGAGGTAGTTT+AGG + tig0022985:26224-26243 Msa1403970:three_prime_UTR 35.0%
!! GTTCATGGATTGGTATCTTT+GGG - tig0022985:24998-25017 None:intergenic 35.0%
!! TGTTCATGGATTGGTATCTT+TGG - tig0022985:24999-25018 None:intergenic 35.0%
!! AGGTTTTGATGTTCATGGAT+TGG - tig0022985:25008-25027 None:intergenic 35.0%
ACATCAAAACCTCACTGTTT+AGG + tig0022985:25016-25035 Msa1403970:five_prime_UTR 35.0%
CATCAAAACCTCACTGTTTA+GGG + tig0022985:25017-25036 Msa1403970:five_prime_UTR 35.0%
TGATGAGGCTATTATTTGTC+AGG + tig0022985:25041-25060 Msa1403970:five_prime_UTR 35.0%
! AGGGTGAAATTTGAGTTTGT+GGG - tig0022985:25179-25198 None:intergenic 35.0%
! AAGGGTGAAATTTGAGTTTG+TGG - tig0022985:25180-25199 None:intergenic 35.0%
! TGTTGTTGTTGTTACGAGAA+AGG - tig0022985:25222-25241 None:intergenic 35.0%
ATCCAAACTCTCTTTGTCTT+TGG + tig0022985:25268-25287 Msa1403970:five_prime_UTR 35.0%
AACGGAAATTTGTCAGTTTG+AGG - tig0022985:25319-25338 None:intergenic 35.0%
TCAAACTGACAAATTTCCGT+TGG + tig0022985:25318-25337 Msa1403970:CDS 35.0%
CAAATTTCCGTTGGATACAA+CGG + tig0022985:25327-25346 Msa1403970:CDS 35.0%
CTTATGATAAGGAGAAGAGA+AGG + tig0022985:25482-25501 Msa1403970:CDS 35.0%
AAGGGAACATTGAAGGATTA+AGG - tig0022985:25585-25604 None:intergenic 35.0%
! CATTTGGTGGTTGATTAACA+TGG - tig0022985:25798-25817 None:intergenic 35.0%
GTGAGAAATTGAAGAACTTG+GGG + tig0022985:25841-25860 Msa1403970:CDS 35.0%
TTGGAAGTTCAGATTGAAGT+AGG - tig0022985:25894-25913 None:intergenic 35.0%
TACTTCAATCTGAACTTCCA+AGG + tig0022985:25893-25912 Msa1403970:exon 35.0%
GTGACTGATTGAATCTCAAT+TGG + tig0022985:26011-26030 Msa1403970:three_prime_UTR 35.0%
TGAGTAGTATGAGGTAGTTT+AGG + tig0022985:26224-26243 Msa1403970:three_prime_UTR 35.0%
!! CAGTGAGGTTTTGATGTTCA+TGG - tig0022985:25013-25032 None:intergenic 40.0%
ATTATTTGTCAGGTCACTGC+TGG + tig0022985:25051-25070 Msa1403970:five_prime_UTR 40.0%
AATGAGTGAAGGAAGCTTCT+AGG - tig0022985:25150-25169 None:intergenic 40.0%
! GGTGAAATTTGAGTTTGTGG+GGG - tig0022985:25177-25196 None:intergenic 40.0%
! GGGTGAAATTTGAGTTTGTG+GGG - tig0022985:25178-25197 None:intergenic 40.0%
! TGTTGTTGTTACGAGAAAGG+TGG - tig0022985:25219-25238 None:intergenic 40.0%
AGCCAAAGACAAAGAGAGTT+TGG - tig0022985:25273-25292 None:intergenic 40.0%
! TCTCTTTGTCTTTGGCTTCA+TGG + tig0022985:25276-25295 Msa1403970:exon 40.0%
! CTCTTTGTCTTTGGCTTCAT+GGG + tig0022985:25277-25296 Msa1403970:exon 40.0%
GTCAGTTTGAGGAGTAGAAA+CGG - tig0022985:25308-25327 None:intergenic 40.0%
TTGGTTTCCGTTGTATCCAA+CGG - tig0022985:25337-25356 None:intergenic 40.0%
AGAAGTTTCGATGGTCTAAG+AGG - tig0022985:25367-25386 None:intergenic 40.0%
ACGTTGAGTAGAAGTTTCGA+TGG - tig0022985:25376-25395 None:intergenic 40.0%
! AATTCGCCAACGGTATTTTC+AGG - tig0022985:25442-25461 None:intergenic 40.0%
AACCTTCACTAATTCGCCAA+CGG - tig0022985:25452-25471 None:intergenic 40.0%
GAAGAGAAGGCCATTATTGA+AGG + tig0022985:25495-25514 Msa1403970:CDS 40.0%
TGAATGGAGAGAAGTGAAGT+TGG - tig0022985:25540-25559 None:intergenic 40.0%
CAAACCTGCACCATTTCATT+TGG - tig0022985:25814-25833 None:intergenic 40.0%
ATGAAATGGTGCAGGTTTGA+AGG + tig0022985:25815-25834 Msa1403970:intron 40.0%
AATTGAAGAACTTGGGGTCA+AGG + tig0022985:25847-25866 Msa1403970:CDS 40.0%
CGAAGCTATTGAGTAGTATG+AGG + tig0022985:26215-26234 Msa1403970:three_prime_UTR 40.0%
TGCCCTTATGCAACATGTAA+AGG + tig0022985:26267-26286 Msa1403970:three_prime_UTR 40.0%
!! CAGTGAGGTTTTGATGTTCA+TGG - tig0022985:25013-25032 None:intergenic 40.0%
ATTATTTGTCAGGTCACTGC+TGG + tig0022985:25051-25070 Msa1403970:five_prime_UTR 40.0%
AATGAGTGAAGGAAGCTTCT+AGG - tig0022985:25150-25169 None:intergenic 40.0%
! GGTGAAATTTGAGTTTGTGG+GGG - tig0022985:25177-25196 None:intergenic 40.0%
! GGGTGAAATTTGAGTTTGTG+GGG - tig0022985:25178-25197 None:intergenic 40.0%
! TGTTGTTGTTACGAGAAAGG+TGG - tig0022985:25219-25238 None:intergenic 40.0%
AGCCAAAGACAAAGAGAGTT+TGG - tig0022985:25273-25292 None:intergenic 40.0%
! TCTCTTTGTCTTTGGCTTCA+TGG + tig0022985:25276-25295 Msa1403970:exon 40.0%
! CTCTTTGTCTTTGGCTTCAT+GGG + tig0022985:25277-25296 Msa1403970:exon 40.0%
GTCAGTTTGAGGAGTAGAAA+CGG - tig0022985:25308-25327 None:intergenic 40.0%
TTGGTTTCCGTTGTATCCAA+CGG - tig0022985:25337-25356 None:intergenic 40.0%
AGAAGTTTCGATGGTCTAAG+AGG - tig0022985:25367-25386 None:intergenic 40.0%
ACGTTGAGTAGAAGTTTCGA+TGG - tig0022985:25376-25395 None:intergenic 40.0%
! AATTCGCCAACGGTATTTTC+AGG - tig0022985:25442-25461 None:intergenic 40.0%
AACCTTCACTAATTCGCCAA+CGG - tig0022985:25452-25471 None:intergenic 40.0%
GAAGAGAAGGCCATTATTGA+AGG + tig0022985:25495-25514 Msa1403970:CDS 40.0%
TGAATGGAGAGAAGTGAAGT+TGG - tig0022985:25540-25559 None:intergenic 40.0%
CAAACCTGCACCATTTCATT+TGG - tig0022985:25814-25833 None:intergenic 40.0%
ATGAAATGGTGCAGGTTTGA+AGG + tig0022985:25815-25834 Msa1403970:intron 40.0%
AATTGAAGAACTTGGGGTCA+AGG + tig0022985:25847-25866 Msa1403970:CDS 40.0%
CGAAGCTATTGAGTAGTATG+AGG + tig0022985:26215-26234 Msa1403970:three_prime_UTR 40.0%
TGCCCTTATGCAACATGTAA+AGG + tig0022985:26267-26286 Msa1403970:three_prime_UTR 40.0%
CTCATCATCCCTAAACAGTG+AGG - tig0022985:25028-25047 None:intergenic 45.0%
CTCACTGTTTAGGGATGATG+AGG + tig0022985:25026-25045 Msa1403970:five_prime_UTR 45.0%
CAGGTCACTGCTGGAAAATT+CGG + tig0022985:25060-25079 Msa1403970:five_prime_UTR 45.0%
AGGTCACTGCTGGAAAATTC+GGG + tig0022985:25061-25080 Msa1403970:five_prime_UTR 45.0%
! TTTGTCTTTGGCTTCATGGG+TGG + tig0022985:25280-25299 Msa1403970:exon 45.0%
TGGTCTAAGAGGAGAGTCAT+TGG - tig0022985:25356-25375 None:intergenic 45.0%
ATGTCGCCTGAAAATACCGT+TGG + tig0022985:25433-25452 Msa1403970:CDS 45.0%
TACCGTTGGCGAATTAGTGA+AGG + tig0022985:25447-25466 Msa1403970:CDS 45.0%
! GGATCGGTGTCCTTCAATAA+TGG - tig0022985:25508-25527 None:intergenic 45.0%
!! AAGTTGGTAGCGGTTTGGAT+CGG - tig0022985:25524-25543 None:intergenic 45.0%
!! AAGTGAAGTTGGTAGCGGTT+TGG - tig0022985:25529-25548 None:intergenic 45.0%
!! GAGAGAAGTGAAGTTGGTAG+CGG - tig0022985:25534-25553 None:intergenic 45.0%
ACCACCAAATGAAATGGTGC+AGG + tig0022985:25807-25826 Msa1403970:intron 45.0%
ACCTGCACCATTTCATTTGG+TGG - tig0022985:25811-25830 None:intergenic 45.0%
TGAACTTCCAAGGCAGCATA+TGG + tig0022985:25903-25922 Msa1403970:exon 45.0%
TCAATTGCCATATGCTGCCT+TGG - tig0022985:25913-25932 None:intergenic 45.0%
! TTGGCCACTCAACACTTTTG+AGG + tig0022985:26030-26049 Msa1403970:three_prime_UTR 45.0%
! TGGCCACTCAACACTTTTGA+GGG + tig0022985:26031-26050 Msa1403970:three_prime_UTR 45.0%
!! TGTCCCTCAAAAGTGTTGAG+TGG - tig0022985:26037-26056 None:intergenic 45.0%
CTCATCATCCCTAAACAGTG+AGG - tig0022985:25028-25047 None:intergenic 45.0%
CTCACTGTTTAGGGATGATG+AGG + tig0022985:25026-25045 Msa1403970:five_prime_UTR 45.0%
CAGGTCACTGCTGGAAAATT+CGG + tig0022985:25060-25079 Msa1403970:five_prime_UTR 45.0%
AGGTCACTGCTGGAAAATTC+GGG + tig0022985:25061-25080 Msa1403970:five_prime_UTR 45.0%
! TTTGTCTTTGGCTTCATGGG+TGG + tig0022985:25280-25299 Msa1403970:exon 45.0%
TGGTCTAAGAGGAGAGTCAT+TGG - tig0022985:25356-25375 None:intergenic 45.0%
ATGTCGCCTGAAAATACCGT+TGG + tig0022985:25433-25452 Msa1403970:CDS 45.0%
TACCGTTGGCGAATTAGTGA+AGG + tig0022985:25447-25466 Msa1403970:CDS 45.0%
! GGATCGGTGTCCTTCAATAA+TGG - tig0022985:25508-25527 None:intergenic 45.0%
!! AAGTTGGTAGCGGTTTGGAT+CGG - tig0022985:25524-25543 None:intergenic 45.0%
!! AAGTGAAGTTGGTAGCGGTT+TGG - tig0022985:25529-25548 None:intergenic 45.0%
!! GAGAGAAGTGAAGTTGGTAG+CGG - tig0022985:25534-25553 None:intergenic 45.0%
ACCACCAAATGAAATGGTGC+AGG + tig0022985:25807-25826 Msa1403970:intron 45.0%
ACCTGCACCATTTCATTTGG+TGG - tig0022985:25811-25830 None:intergenic 45.0%
TGAACTTCCAAGGCAGCATA+TGG + tig0022985:25903-25922 Msa1403970:exon 45.0%
TCAATTGCCATATGCTGCCT+TGG - tig0022985:25913-25932 None:intergenic 45.0%
! TTGGCCACTCAACACTTTTG+AGG + tig0022985:26030-26049 Msa1403970:three_prime_UTR 45.0%
! TGGCCACTCAACACTTTTGA+GGG + tig0022985:26031-26050 Msa1403970:three_prime_UTR 45.0%
!! TGTCCCTCAAAAGTGTTGAG+TGG - tig0022985:26037-26056 None:intergenic 45.0%
GTGGGGGCTTAAATGAGTGA+AGG - tig0022985:25161-25180 None:intergenic 50.0%
GTGTCCATCGAGAACACGTT+GGG + tig0022985:25394-25413 Msa1403970:CDS 50.0%
GTGGGGGCTTAAATGAGTGA+AGG - tig0022985:25161-25180 None:intergenic 50.0%
GTGTCCATCGAGAACACGTT+GGG + tig0022985:25394-25413 Msa1403970:CDS 50.0%
CGTGTCCATCGAGAACACGT+TGG + tig0022985:25393-25412 Msa1403970:CDS 55.0%
TGCACCCAACGTGTTCTCGA+TGG - tig0022985:25401-25420 None:intergenic 55.0%
CGTGTCCATCGAGAACACGT+TGG + tig0022985:25393-25412 Msa1403970:CDS 55.0%
TGCACCCAACGTGTTCTCGA+TGG - tig0022985:25401-25420 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0022985 gene 24998 26304 24998 ID=Msa1403970;Name=Msa1403970
tig0022985 mRNA 24998 26304 24998 ID=Msa1403970-mRNA-1;Parent=Msa1403970;Name=Msa1403970-mRNA-1;_AED=0.00;_eAED=0.00;_QI=297|1|1|1|1|1|2|399|116
tig0022985 exon 24998 25568 24998 ID=Msa1403970-mRNA-1:exon:19531;Parent=Msa1403970-mRNA-1
tig0022985 exon 25829 26304 25829 ID=Msa1403970-mRNA-1:exon:19532;Parent=Msa1403970-mRNA-1
tig0022985 five_prime_UTR 24998 25294 24998 ID=Msa1403970-mRNA-1:five_prime_utr;Parent=Msa1403970-mRNA-1
tig0022985 CDS 25295 25568 25295 ID=Msa1403970-mRNA-1:cds;Parent=Msa1403970-mRNA-1
tig0022985 CDS 25829 25905 25829 ID=Msa1403970-mRNA-1:cds;Parent=Msa1403970-mRNA-1
tig0022985 three_prime_UTR 25906 26304 25906 ID=Msa1403970-mRNA-1:three_prime_utr;Parent=Msa1403970-mRNA-1
Gene Sequence

>Msa1403970

ATGGGTGGTACCGTTTCTACTCCTCAAACTGACAAATTTCCGTTGGATACAACGGAAACCAATGACTCTCCTCTTAGACCATCGAAACTTCTACTCAACGTGTCCATCGAGAACACGTTGGGTGCAATACAAGTTTTGATGTCGCCTGAAAATACCGTTGGCGAATTAGTGAAGGTTGCATTGATGACTTATGATAAGGAGAAGAGAAGGCCATTATTGAAGGACACCGATCCAAACCGCTACCAACTTCACTTCTCTCCATTCACGCTTGAAAGTTTGAAGGTTAGTGAGAAATTGAAGAACTTGGGGTCAAGGAACTTCTTTCTATGCTCAAAGCCTACTTCAATCTGA
Protein sequence

>Msa1403970

MGGTVSTPQTDKFPLDTTETNDSPLRPSKLLLNVSIENTLGAIQVLMSPENTVGELVKVALMTYDKEKRRPLLKDTDPNRYQLHFSPFTLESLKVSEKLKNLGSRNFFLCSKPTSI*