AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0096000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0096000 MtrunA17_Chr5g0398651 93.966 116 7 0 1 116 1 116 9.08e-77 222
Msa0096000 MtrunA17_Chr4g0056791 62.385 109 36 1 5 113 41 144 6.96e-40 130
Msa0096000 MtrunA17_Chr2g0320881 53.125 96 45 0 20 115 51 146 7.09e-27 98.6
Msa0096000 MtrunA17_Chr4g0027171 50.562 89 44 0 24 112 67 155 6.46e-26 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0096000 AT2G27830.1 52.128 94 45 0 19 112 61 154 1.09e-30 108

Find 70 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGGCAACTAAATATAATT+TGG 0.142333 1_3:+1428496 None:intergenic
AAGATATTATTCACACTTAT+TGG 0.154119 1_3:-1428795 Msa0096000:three_prime_UTR
ATCCAAACTCTCTTTGTCTT+TGG 0.236659 1_3:-1429479 Msa0096000:five_prime_UTR
TCTCTTTGTCTTTGGCTTCA+TGG 0.240774 1_3:-1429471 Msa0096000:exon
ACATCAAAACCTCACTGTTT+AGG 0.276948 1_3:-1429731 Msa0096000:five_prime_UTR
AATCACTTTAGCATTAATTA+TGG 0.277764 1_3:+1428765 None:intergenic
CTCTTTGTCTTTGGCTTCAT+GGG 0.284185 1_3:-1429470 Msa0096000:exon
CAGGTCACTGCTGGAAAATT+CGG 0.287630 1_3:-1429687 Msa0096000:five_prime_UTR
AATGAGTGAAGGAAGCTTCT+AGG 0.300519 1_3:+1429600 None:intergenic
CATCAAAACCTCACTGTTTA+GGG 0.313886 1_3:-1429730 Msa0096000:five_prime_UTR
AGCCAAAGACAAAGAGAGTT+TGG 0.330320 1_3:+1429477 None:intergenic
TATCCTTTACATGTTGCATA+AGG 0.355932 1_3:+1428477 None:intergenic
ATGTATCTTGTGAAAGGTTA+AGG 0.390025 1_3:-1428683 Msa0096000:three_prime_UTR
AGGGCAACTAAATATAATTT+GGG 0.412005 1_3:+1428497 None:intergenic
ATTATTTGTCAGGTCACTGC+TGG 0.415487 1_3:-1429696 Msa0096000:five_prime_UTR
TGCATTGATGACTTATGATA+AGG 0.419305 1_3:-1429276 Msa0096000:CDS
AACGGAAATTTGTCAGTTTG+AGG 0.425120 1_3:+1429431 None:intergenic
TAGTGAGAAATTGAAGAACT+TGG 0.435583 1_3:-1428908 Msa0096000:CDS
TGAGTAGTATGAGGTAGTTT+AGG 0.435990 1_3:-1428523 Msa0096000:three_prime_UTR
TAATTACTTTCAAGCGTGAA+TGG 0.437696 1_3:+1429194 None:intergenic
TGATGAGGCTATTATTTGTC+AGG 0.438001 1_3:-1429706 Msa0096000:five_prime_UTR
AGAGTTTGGATTTCAGTTTA+CGG 0.441806 1_3:+1429491 None:intergenic
ATGAAATGGTGCAGGTTTGA+AGG 0.447179 1_3:-1428932 Msa0096000:intron
ATCCTTTACATGTTGCATAA+GGG 0.449046 1_3:+1428478 None:intergenic
GGATCGGTGTCCTTCAATAA+TGG 0.452970 1_3:+1429242 None:intergenic
GTGACTGATTGAATCTCAAT+TGG 0.460220 1_3:-1428736 Msa0096000:three_prime_UTR
AAGTGAAGTTGGTAGCGGTT+TGG 0.461676 1_3:+1429221 None:intergenic
TGCCCTTATGCAACATGTAA+AGG 0.473630 1_3:-1428480 Msa0096000:three_prime_UTR
TGAACTTCCAAGGCAGCATA+TGG 0.474963 1_3:-1428844 Msa0096000:three_prime_UTR
AGTGAGAAATTGAAGAACTT+GGG 0.475992 1_3:-1428907 Msa0096000:CDS
AAGGGTGAAATTTGAGTTTG+TGG 0.476387 1_3:+1429570 None:intergenic
TGAATGGAGAGAAGTGAAGT+TGG 0.482669 1_3:+1429210 None:intergenic
TTGGAAGTTCAGATTGAAGT+AGG 0.484151 1_3:+1428856 None:intergenic
AATTAATGCTAAAGTGATTG+AGG 0.488321 1_3:-1428761 Msa0096000:three_prime_UTR
GAAGAGAAGGCCATTATTGA+AGG 0.491115 1_3:-1429252 Msa0096000:CDS
AGGTCACTGCTGGAAAATTC+GGG 0.491361 1_3:-1429686 Msa0096000:five_prime_UTR
ATATTTCGATTTGTAGAGGT+TGG 0.493266 1_3:+1429642 None:intergenic
AGGGTGAAATTTGAGTTTGT+GGG 0.494506 1_3:+1429571 None:intergenic
AATTGAAGAACTTGGGGTCA+AGG 0.499762 1_3:-1428900 Msa0096000:CDS
TCAATTGCCATATGCTGCCT+TGG 0.502902 1_3:+1428837 None:intergenic
TGGTCTAAGAGGAGAGTCAT+TGG 0.503838 1_3:+1429394 None:intergenic
TGTTGTTGTTGTTACGAGAA+AGG 0.503927 1_3:+1429528 None:intergenic
AAATTTATGAATCGTCAAGC+TGG 0.516087 1_3:-1428555 Msa0096000:three_prime_UTR
AATCTTATGTATCTTGTGAA+AGG 0.518070 1_3:-1428689 Msa0096000:three_prime_UTR
AGAAGTTTCGATGGTCTAAG+AGG 0.521243 1_3:+1429383 None:intergenic
GAGAGAAGTGAAGTTGGTAG+CGG 0.523441 1_3:+1429216 None:intergenic
CGAAGCTATTGAGTAGTATG+AGG 0.524961 1_3:-1428532 Msa0096000:three_prime_UTR
TTTGTCTTTGGCTTCATGGG+TGG 0.526303 1_3:-1429467 Msa0096000:exon
TCAAACTGACAAATTTCCGT+TGG 0.528724 1_3:-1429429 Msa0096000:CDS
GTCAGTTTGAGGAGTAGAAA+CGG 0.529246 1_3:+1429442 None:intergenic
GTGTCCATCGAGAACACGTT+GGG 0.537604 1_3:-1429353 Msa0096000:CDS
CAAATTTCCGTTGGATACAA+CGG 0.549881 1_3:-1429420 Msa0096000:CDS
TTGGTTTCCGTTGTATCCAA+CGG 0.551596 1_3:+1429413 None:intergenic
TACCGTTGGCGAATTAGTGA+AGG 0.567706 1_3:-1429300 Msa0096000:CDS
GGGTGAAATTTGAGTTTGTG+GGG 0.570497 1_3:+1429572 None:intergenic
ACTTATATTTCGATTTGTAG+AGG 0.579774 1_3:+1429638 None:intergenic
GTGAGAAATTGAAGAACTTG+GGG 0.585031 1_3:-1428906 Msa0096000:CDS
CTTATGATAAGGAGAAGAGA+AGG 0.588206 1_3:-1429265 Msa0096000:CDS
AAGTTGGTAGCGGTTTGGAT+CGG 0.590772 1_3:+1429226 None:intergenic
CTCACTGTTTAGGGATGATG+AGG 0.592643 1_3:-1429721 Msa0096000:five_prime_UTR
ATGTCGCCTGAAAATACCGT+TGG 0.599343 1_3:-1429314 Msa0096000:CDS
TGCACCCAACGTGTTCTCGA+TGG 0.600223 1_3:+1429349 None:intergenic
TACTTCAATCTGAACTTCCA+AGG 0.601898 1_3:-1428854 Msa0096000:exon
TGTCCCTCAAAAGTGTTGAG+TGG 0.609379 1_3:+1428713 None:intergenic
GGTGAAATTTGAGTTTGTGG+GGG 0.618265 1_3:+1429573 None:intergenic
ACGTTGAGTAGAAGTTTCGA+TGG 0.635634 1_3:+1429374 None:intergenic
TGTTGTTGTTACGAGAAAGG+TGG 0.636326 1_3:+1429531 None:intergenic
AACCTTCACTAATTCGCCAA+CGG 0.655659 1_3:+1429298 None:intergenic
CGTGTCCATCGAGAACACGT+TGG 0.692755 1_3:-1429354 Msa0096000:CDS
CTCATCATCCCTAAACAGTG+AGG 0.694635 1_3:+1429722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGATATAAATTATGAAAAAA+GGG + chr1_3:1429070-1429089 None:intergenic 15.0%
!! AGGATATAAATTATGAAAAA+AGG + chr1_3:1429071-1429090 None:intergenic 15.0%
!! AAAAATGATATGAAAAAACA+AGG + chr1_3:1429091-1429110 None:intergenic 15.0%
!! AAGTAAAAAATAATACTGTT+TGG - chr1_3:1429568-1429587 Msa0096000:five_prime_UTR 15.0%
!! GGATATAAATTATGAAAAAA+GGG + chr1_3:1429070-1429089 None:intergenic 15.0%
!! AGGATATAAATTATGAAAAA+AGG + chr1_3:1429071-1429090 None:intergenic 15.0%
!! AAAAATGATATGAAAAAACA+AGG + chr1_3:1429091-1429110 None:intergenic 15.0%
!! AAGTAAAAAATAATACTGTT+TGG - chr1_3:1429568-1429587 Msa0096000:five_prime_UTR 15.0%
!! AAGATATTATTCACACTTAT+TGG - chr1_3:1429419-1429438 Msa0096000:CDS 20.0%
!! AATCACTTTAGCATTAATTA+TGG + chr1_3:1429452-1429471 None:intergenic 20.0%
!! AAGATATTATTCACACTTAT+TGG - chr1_3:1429419-1429438 Msa0096000:CDS 20.0%
!! AATCACTTTAGCATTAATTA+TGG + chr1_3:1429452-1429471 None:intergenic 20.0%
!! ACTTATATTTCGATTTGTAG+AGG + chr1_3:1428579-1428598 None:intergenic 25.0%
!!! GGTTTTTTTCAAAAGTGAAA+GGG + chr1_3:1428665-1428684 None:intergenic 25.0%
!!! TGGTTTTTTTCAAAAGTGAA+AGG + chr1_3:1428666-1428685 None:intergenic 25.0%
! AATTAATGCTAAAGTGATTG+AGG - chr1_3:1429453-1429472 Msa0096000:CDS 25.0%
! AATCTTATGTATCTTGTGAA+AGG - chr1_3:1429525-1429544 Msa0096000:five_prime_UTR 25.0%
! AGGGCAACTAAATATAATTT+GGG + chr1_3:1429720-1429739 None:intergenic 25.0%
! AAGGGCAACTAAATATAATT+TGG + chr1_3:1429721-1429740 None:intergenic 25.0%
!! ACTTATATTTCGATTTGTAG+AGG + chr1_3:1428579-1428598 None:intergenic 25.0%
!!! GGTTTTTTTCAAAAGTGAAA+GGG + chr1_3:1428665-1428684 None:intergenic 25.0%
!!! TGGTTTTTTTCAAAAGTGAA+AGG + chr1_3:1428666-1428685 None:intergenic 25.0%
! AATTAATGCTAAAGTGATTG+AGG - chr1_3:1429453-1429472 Msa0096000:CDS 25.0%
! AATCTTATGTATCTTGTGAA+AGG - chr1_3:1429525-1429544 Msa0096000:five_prime_UTR 25.0%
! AGGGCAACTAAATATAATTT+GGG + chr1_3:1429720-1429739 None:intergenic 25.0%
! AAGGGCAACTAAATATAATT+TGG + chr1_3:1429721-1429740 None:intergenic 25.0%
! ATATTTCGATTTGTAGAGGT+TGG + chr1_3:1428575-1428594 None:intergenic 30.0%
AGAGTTTGGATTTCAGTTTA+CGG + chr1_3:1428726-1428745 None:intergenic 30.0%
! TGCATTGATGACTTATGATA+AGG - chr1_3:1428938-1428957 Msa0096000:intron 30.0%
TAATTACTTTCAAGCGTGAA+TGG + chr1_3:1429023-1429042 None:intergenic 30.0%
ATGAAAAAAGGGAACATTGA+AGG + chr1_3:1429059-1429078 None:intergenic 30.0%
TATACACGATGCATATGATA+AGG + chr1_3:1429223-1429242 None:intergenic 30.0%
TAATCAACCACCAAATGAAA+TGG - chr1_3:1429268-1429287 Msa0096000:CDS 30.0%
TAGTGAGAAATTGAAGAACT+TGG - chr1_3:1429306-1429325 Msa0096000:CDS 30.0%
AGTGAGAAATTGAAGAACTT+GGG - chr1_3:1429307-1429326 Msa0096000:CDS 30.0%
ATGTATCTTGTGAAAGGTTA+AGG - chr1_3:1429531-1429550 Msa0096000:five_prime_UTR 30.0%
! AATCTAGCTGCATGTATTTT+TGG - chr1_3:1429600-1429619 Msa0096000:five_prime_UTR 30.0%
AAATTTATGAATCGTCAAGC+TGG - chr1_3:1429659-1429678 Msa0096000:five_prime_UTR 30.0%
ATCCTTTACATGTTGCATAA+GGG + chr1_3:1429739-1429758 None:intergenic 30.0%
TATCCTTTACATGTTGCATA+AGG + chr1_3:1429740-1429759 None:intergenic 30.0%
! ATATTTCGATTTGTAGAGGT+TGG + chr1_3:1428575-1428594 None:intergenic 30.0%
AGAGTTTGGATTTCAGTTTA+CGG + chr1_3:1428726-1428745 None:intergenic 30.0%
! TGCATTGATGACTTATGATA+AGG - chr1_3:1428938-1428957 Msa0096000:intron 30.0%
TAATTACTTTCAAGCGTGAA+TGG + chr1_3:1429023-1429042 None:intergenic 30.0%
ATGAAAAAAGGGAACATTGA+AGG + chr1_3:1429059-1429078 None:intergenic 30.0%
TATACACGATGCATATGATA+AGG + chr1_3:1429223-1429242 None:intergenic 30.0%
TAATCAACCACCAAATGAAA+TGG - chr1_3:1429268-1429287 Msa0096000:CDS 30.0%
TAGTGAGAAATTGAAGAACT+TGG - chr1_3:1429306-1429325 Msa0096000:CDS 30.0%
AGTGAGAAATTGAAGAACTT+GGG - chr1_3:1429307-1429326 Msa0096000:CDS 30.0%
ATGTATCTTGTGAAAGGTTA+AGG - chr1_3:1429531-1429550 Msa0096000:five_prime_UTR 30.0%
! AATCTAGCTGCATGTATTTT+TGG - chr1_3:1429600-1429619 Msa0096000:five_prime_UTR 30.0%
AAATTTATGAATCGTCAAGC+TGG - chr1_3:1429659-1429678 Msa0096000:five_prime_UTR 30.0%
ATCCTTTACATGTTGCATAA+GGG + chr1_3:1429739-1429758 None:intergenic 30.0%
TATCCTTTACATGTTGCATA+AGG + chr1_3:1429740-1429759 None:intergenic 30.0%
!! AGGTTTTGATGTTCATGGAT+TGG + chr1_3:1428475-1428494 None:intergenic 35.0%
ACATCAAAACCTCACTGTTT+AGG - chr1_3:1428483-1428502 Msa0096000:three_prime_UTR 35.0%
CATCAAAACCTCACTGTTTA+GGG - chr1_3:1428484-1428503 Msa0096000:three_prime_UTR 35.0%
TGATGAGGCTATTATTTGTC+AGG - chr1_3:1428508-1428527 Msa0096000:three_prime_UTR 35.0%
! AGGGTGAAATTTGAGTTTGT+GGG + chr1_3:1428646-1428665 None:intergenic 35.0%
! AAGGGTGAAATTTGAGTTTG+TGG + chr1_3:1428647-1428666 None:intergenic 35.0%
! TGTTGTTGTTGTTACGAGAA+AGG + chr1_3:1428689-1428708 None:intergenic 35.0%
ATCCAAACTCTCTTTGTCTT+TGG - chr1_3:1428735-1428754 Msa0096000:three_prime_UTR 35.0%
AACGGAAATTTGTCAGTTTG+AGG + chr1_3:1428786-1428805 None:intergenic 35.0%
TCAAACTGACAAATTTCCGT+TGG - chr1_3:1428785-1428804 Msa0096000:three_prime_UTR 35.0%
CAAATTTCCGTTGGATACAA+CGG - chr1_3:1428794-1428813 Msa0096000:three_prime_UTR 35.0%
CTTATGATAAGGAGAAGAGA+AGG - chr1_3:1428949-1428968 Msa0096000:intron 35.0%
AAGGGAACATTGAAGGATTA+AGG + chr1_3:1429052-1429071 None:intergenic 35.0%
! CATTTGGTGGTTGATTAACA+TGG + chr1_3:1429265-1429284 None:intergenic 35.0%
GTGAGAAATTGAAGAACTTG+GGG - chr1_3:1429308-1429327 Msa0096000:CDS 35.0%
TTGGAAGTTCAGATTGAAGT+AGG + chr1_3:1429361-1429380 None:intergenic 35.0%
TACTTCAATCTGAACTTCCA+AGG - chr1_3:1429360-1429379 Msa0096000:CDS 35.0%
GTGACTGATTGAATCTCAAT+TGG - chr1_3:1429478-1429497 Msa0096000:five_prime_UTR 35.0%
TGAGTAGTATGAGGTAGTTT+AGG - chr1_3:1429691-1429710 Msa0096000:five_prime_UTR 35.0%
!! GTTCATGGATTGGTATCTTT+GGG + chr1_3:1428465-1428484 None:intergenic 35.0%
!! TGTTCATGGATTGGTATCTT+TGG + chr1_3:1428466-1428485 None:intergenic 35.0%
!! AGGTTTTGATGTTCATGGAT+TGG + chr1_3:1428475-1428494 None:intergenic 35.0%
ACATCAAAACCTCACTGTTT+AGG - chr1_3:1428483-1428502 Msa0096000:three_prime_UTR 35.0%
CATCAAAACCTCACTGTTTA+GGG - chr1_3:1428484-1428503 Msa0096000:three_prime_UTR 35.0%
TGATGAGGCTATTATTTGTC+AGG - chr1_3:1428508-1428527 Msa0096000:three_prime_UTR 35.0%
! AGGGTGAAATTTGAGTTTGT+GGG + chr1_3:1428646-1428665 None:intergenic 35.0%
! AAGGGTGAAATTTGAGTTTG+TGG + chr1_3:1428647-1428666 None:intergenic 35.0%
! TGTTGTTGTTGTTACGAGAA+AGG + chr1_3:1428689-1428708 None:intergenic 35.0%
ATCCAAACTCTCTTTGTCTT+TGG - chr1_3:1428735-1428754 Msa0096000:three_prime_UTR 35.0%
AACGGAAATTTGTCAGTTTG+AGG + chr1_3:1428786-1428805 None:intergenic 35.0%
TCAAACTGACAAATTTCCGT+TGG - chr1_3:1428785-1428804 Msa0096000:three_prime_UTR 35.0%
CAAATTTCCGTTGGATACAA+CGG - chr1_3:1428794-1428813 Msa0096000:three_prime_UTR 35.0%
CTTATGATAAGGAGAAGAGA+AGG - chr1_3:1428949-1428968 Msa0096000:intron 35.0%
AAGGGAACATTGAAGGATTA+AGG + chr1_3:1429052-1429071 None:intergenic 35.0%
! CATTTGGTGGTTGATTAACA+TGG + chr1_3:1429265-1429284 None:intergenic 35.0%
GTGAGAAATTGAAGAACTTG+GGG - chr1_3:1429308-1429327 Msa0096000:CDS 35.0%
TTGGAAGTTCAGATTGAAGT+AGG + chr1_3:1429361-1429380 None:intergenic 35.0%
TACTTCAATCTGAACTTCCA+AGG - chr1_3:1429360-1429379 Msa0096000:CDS 35.0%
GTGACTGATTGAATCTCAAT+TGG - chr1_3:1429478-1429497 Msa0096000:five_prime_UTR 35.0%
TGAGTAGTATGAGGTAGTTT+AGG - chr1_3:1429691-1429710 Msa0096000:five_prime_UTR 35.0%
!! CAGTGAGGTTTTGATGTTCA+TGG + chr1_3:1428480-1428499 None:intergenic 40.0%
ATTATTTGTCAGGTCACTGC+TGG - chr1_3:1428518-1428537 Msa0096000:three_prime_UTR 40.0%
AATGAGTGAAGGAAGCTTCT+AGG + chr1_3:1428617-1428636 None:intergenic 40.0%
! GGTGAAATTTGAGTTTGTGG+GGG + chr1_3:1428644-1428663 None:intergenic 40.0%
! GGGTGAAATTTGAGTTTGTG+GGG + chr1_3:1428645-1428664 None:intergenic 40.0%
! TGTTGTTGTTACGAGAAAGG+TGG + chr1_3:1428686-1428705 None:intergenic 40.0%
AGCCAAAGACAAAGAGAGTT+TGG + chr1_3:1428740-1428759 None:intergenic 40.0%
! TCTCTTTGTCTTTGGCTTCA+TGG - chr1_3:1428743-1428762 Msa0096000:three_prime_UTR 40.0%
! CTCTTTGTCTTTGGCTTCAT+GGG - chr1_3:1428744-1428763 Msa0096000:three_prime_UTR 40.0%
GTCAGTTTGAGGAGTAGAAA+CGG + chr1_3:1428775-1428794 None:intergenic 40.0%
TTGGTTTCCGTTGTATCCAA+CGG + chr1_3:1428804-1428823 None:intergenic 40.0%
AGAAGTTTCGATGGTCTAAG+AGG + chr1_3:1428834-1428853 None:intergenic 40.0%
ACGTTGAGTAGAAGTTTCGA+TGG + chr1_3:1428843-1428862 None:intergenic 40.0%
! AATTCGCCAACGGTATTTTC+AGG + chr1_3:1428909-1428928 None:intergenic 40.0%
AACCTTCACTAATTCGCCAA+CGG + chr1_3:1428919-1428938 None:intergenic 40.0%
GAAGAGAAGGCCATTATTGA+AGG - chr1_3:1428962-1428981 Msa0096000:intron 40.0%
TGAATGGAGAGAAGTGAAGT+TGG + chr1_3:1429007-1429026 None:intergenic 40.0%
CAAACCTGCACCATTTCATT+TGG + chr1_3:1429281-1429300 None:intergenic 40.0%
ATGAAATGGTGCAGGTTTGA+AGG - chr1_3:1429282-1429301 Msa0096000:CDS 40.0%
AATTGAAGAACTTGGGGTCA+AGG - chr1_3:1429314-1429333 Msa0096000:CDS 40.0%
CGAAGCTATTGAGTAGTATG+AGG - chr1_3:1429682-1429701 Msa0096000:five_prime_UTR 40.0%
TGCCCTTATGCAACATGTAA+AGG - chr1_3:1429734-1429753 Msa0096000:five_prime_UTR 40.0%
!! CAGTGAGGTTTTGATGTTCA+TGG + chr1_3:1428480-1428499 None:intergenic 40.0%
ATTATTTGTCAGGTCACTGC+TGG - chr1_3:1428518-1428537 Msa0096000:three_prime_UTR 40.0%
AATGAGTGAAGGAAGCTTCT+AGG + chr1_3:1428617-1428636 None:intergenic 40.0%
! GGTGAAATTTGAGTTTGTGG+GGG + chr1_3:1428644-1428663 None:intergenic 40.0%
! GGGTGAAATTTGAGTTTGTG+GGG + chr1_3:1428645-1428664 None:intergenic 40.0%
! TGTTGTTGTTACGAGAAAGG+TGG + chr1_3:1428686-1428705 None:intergenic 40.0%
AGCCAAAGACAAAGAGAGTT+TGG + chr1_3:1428740-1428759 None:intergenic 40.0%
! TCTCTTTGTCTTTGGCTTCA+TGG - chr1_3:1428743-1428762 Msa0096000:three_prime_UTR 40.0%
! CTCTTTGTCTTTGGCTTCAT+GGG - chr1_3:1428744-1428763 Msa0096000:three_prime_UTR 40.0%
GTCAGTTTGAGGAGTAGAAA+CGG + chr1_3:1428775-1428794 None:intergenic 40.0%
TTGGTTTCCGTTGTATCCAA+CGG + chr1_3:1428804-1428823 None:intergenic 40.0%
AGAAGTTTCGATGGTCTAAG+AGG + chr1_3:1428834-1428853 None:intergenic 40.0%
ACGTTGAGTAGAAGTTTCGA+TGG + chr1_3:1428843-1428862 None:intergenic 40.0%
! AATTCGCCAACGGTATTTTC+AGG + chr1_3:1428909-1428928 None:intergenic 40.0%
AACCTTCACTAATTCGCCAA+CGG + chr1_3:1428919-1428938 None:intergenic 40.0%
GAAGAGAAGGCCATTATTGA+AGG - chr1_3:1428962-1428981 Msa0096000:intron 40.0%
TGAATGGAGAGAAGTGAAGT+TGG + chr1_3:1429007-1429026 None:intergenic 40.0%
CAAACCTGCACCATTTCATT+TGG + chr1_3:1429281-1429300 None:intergenic 40.0%
ATGAAATGGTGCAGGTTTGA+AGG - chr1_3:1429282-1429301 Msa0096000:CDS 40.0%
AATTGAAGAACTTGGGGTCA+AGG - chr1_3:1429314-1429333 Msa0096000:CDS 40.0%
CGAAGCTATTGAGTAGTATG+AGG - chr1_3:1429682-1429701 Msa0096000:five_prime_UTR 40.0%
TGCCCTTATGCAACATGTAA+AGG - chr1_3:1429734-1429753 Msa0096000:five_prime_UTR 40.0%
CTCATCATCCCTAAACAGTG+AGG + chr1_3:1428495-1428514 None:intergenic 45.0%
CTCACTGTTTAGGGATGATG+AGG - chr1_3:1428493-1428512 Msa0096000:three_prime_UTR 45.0%
CAGGTCACTGCTGGAAAATT+CGG - chr1_3:1428527-1428546 Msa0096000:three_prime_UTR 45.0%
AGGTCACTGCTGGAAAATTC+GGG - chr1_3:1428528-1428547 Msa0096000:three_prime_UTR 45.0%
! TTTGTCTTTGGCTTCATGGG+TGG - chr1_3:1428747-1428766 Msa0096000:three_prime_UTR 45.0%
TGGTCTAAGAGGAGAGTCAT+TGG + chr1_3:1428823-1428842 None:intergenic 45.0%
ATGTCGCCTGAAAATACCGT+TGG - chr1_3:1428900-1428919 Msa0096000:CDS 45.0%
TACCGTTGGCGAATTAGTGA+AGG - chr1_3:1428914-1428933 Msa0096000:CDS 45.0%
! GGATCGGTGTCCTTCAATAA+TGG + chr1_3:1428975-1428994 None:intergenic 45.0%
!! AAGTTGGTAGCGGTTTGGAT+CGG + chr1_3:1428991-1429010 None:intergenic 45.0%
!! AAGTGAAGTTGGTAGCGGTT+TGG + chr1_3:1428996-1429015 None:intergenic 45.0%
!! GAGAGAAGTGAAGTTGGTAG+CGG + chr1_3:1429001-1429020 None:intergenic 45.0%
ACCACCAAATGAAATGGTGC+AGG - chr1_3:1429274-1429293 Msa0096000:CDS 45.0%
ACCTGCACCATTTCATTTGG+TGG + chr1_3:1429278-1429297 None:intergenic 45.0%
TGAACTTCCAAGGCAGCATA+TGG - chr1_3:1429370-1429389 Msa0096000:CDS 45.0%
TCAATTGCCATATGCTGCCT+TGG + chr1_3:1429380-1429399 None:intergenic 45.0%
! TTGGCCACTCAACACTTTTG+AGG - chr1_3:1429497-1429516 Msa0096000:five_prime_UTR 45.0%
! TGGCCACTCAACACTTTTGA+GGG - chr1_3:1429498-1429517 Msa0096000:five_prime_UTR 45.0%
!! TGTCCCTCAAAAGTGTTGAG+TGG + chr1_3:1429504-1429523 None:intergenic 45.0%
CTCATCATCCCTAAACAGTG+AGG + chr1_3:1428495-1428514 None:intergenic 45.0%
CTCACTGTTTAGGGATGATG+AGG - chr1_3:1428493-1428512 Msa0096000:three_prime_UTR 45.0%
CAGGTCACTGCTGGAAAATT+CGG - chr1_3:1428527-1428546 Msa0096000:three_prime_UTR 45.0%
AGGTCACTGCTGGAAAATTC+GGG - chr1_3:1428528-1428547 Msa0096000:three_prime_UTR 45.0%
! TTTGTCTTTGGCTTCATGGG+TGG - chr1_3:1428747-1428766 Msa0096000:three_prime_UTR 45.0%
TGGTCTAAGAGGAGAGTCAT+TGG + chr1_3:1428823-1428842 None:intergenic 45.0%
ATGTCGCCTGAAAATACCGT+TGG - chr1_3:1428900-1428919 Msa0096000:CDS 45.0%
TACCGTTGGCGAATTAGTGA+AGG - chr1_3:1428914-1428933 Msa0096000:CDS 45.0%
! GGATCGGTGTCCTTCAATAA+TGG + chr1_3:1428975-1428994 None:intergenic 45.0%
!! AAGTTGGTAGCGGTTTGGAT+CGG + chr1_3:1428991-1429010 None:intergenic 45.0%
!! AAGTGAAGTTGGTAGCGGTT+TGG + chr1_3:1428996-1429015 None:intergenic 45.0%
!! GAGAGAAGTGAAGTTGGTAG+CGG + chr1_3:1429001-1429020 None:intergenic 45.0%
ACCACCAAATGAAATGGTGC+AGG - chr1_3:1429274-1429293 Msa0096000:CDS 45.0%
ACCTGCACCATTTCATTTGG+TGG + chr1_3:1429278-1429297 None:intergenic 45.0%
TGAACTTCCAAGGCAGCATA+TGG - chr1_3:1429370-1429389 Msa0096000:CDS 45.0%
TCAATTGCCATATGCTGCCT+TGG + chr1_3:1429380-1429399 None:intergenic 45.0%
! TTGGCCACTCAACACTTTTG+AGG - chr1_3:1429497-1429516 Msa0096000:five_prime_UTR 45.0%
! TGGCCACTCAACACTTTTGA+GGG - chr1_3:1429498-1429517 Msa0096000:five_prime_UTR 45.0%
!! TGTCCCTCAAAAGTGTTGAG+TGG + chr1_3:1429504-1429523 None:intergenic 45.0%
GTGGGGGCTTAAATGAGTGA+AGG + chr1_3:1428628-1428647 None:intergenic 50.0%
GTGTCCATCGAGAACACGTT+GGG - chr1_3:1428861-1428880 Msa0096000:exon 50.0%
GTGGGGGCTTAAATGAGTGA+AGG + chr1_3:1428628-1428647 None:intergenic 50.0%
GTGTCCATCGAGAACACGTT+GGG - chr1_3:1428861-1428880 Msa0096000:exon 50.0%
CGTGTCCATCGAGAACACGT+TGG - chr1_3:1428860-1428879 Msa0096000:exon 55.0%
TGCACCCAACGTGTTCTCGA+TGG + chr1_3:1428868-1428887 None:intergenic 55.0%
CGTGTCCATCGAGAACACGT+TGG - chr1_3:1428860-1428879 Msa0096000:exon 55.0%
TGCACCCAACGTGTTCTCGA+TGG + chr1_3:1428868-1428887 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 1428465 1429771 1428465 ID=Msa0096000;Name=Msa0096000
chr1_3 mRNA 1428465 1429771 1428465 ID=Msa0096000-mRNA-1;Parent=Msa0096000;Name=Msa0096000-mRNA-1;_AED=0.00;_eAED=0.00;_QI=297|1|1|1|1|1|2|399|116
chr1_3 exon 1428465 1428940 1428465 ID=Msa0096000-mRNA-1:exon:308;Parent=Msa0096000-mRNA-1
chr1_3 exon 1429201 1429771 1429201 ID=Msa0096000-mRNA-1:exon:307;Parent=Msa0096000-mRNA-1
chr1_3 five_prime_UTR 1429475 1429771 1429475 ID=Msa0096000-mRNA-1:five_prime_utr;Parent=Msa0096000-mRNA-1
chr1_3 CDS 1429201 1429474 1429201 ID=Msa0096000-mRNA-1:cds;Parent=Msa0096000-mRNA-1
chr1_3 CDS 1428864 1428940 1428864 ID=Msa0096000-mRNA-1:cds;Parent=Msa0096000-mRNA-1
chr1_3 three_prime_UTR 1428465 1428863 1428465 ID=Msa0096000-mRNA-1:three_prime_utr;Parent=Msa0096000-mRNA-1
Gene Sequence

>Msa0096000

ATGGGTGGTACCGTTTCTACTCCTCAAACTGACAAATTTCCGTTGGATACAACGGAAACCAATGACTCTCCTCTTAGACCATCGAAACTTCTACTCAACGTGTCCATCGAGAACACGTTGGGTGCAATACAAGTTTTGATGTCGCCTGAAAATACCGTTGGCGAATTAGTGAAGGTTGCATTGATGACTTATGATAAGGAGAAGAGAAGGCCATTATTGAAGGACACCGATCCAAACCGCTACCAACTTCACTTCTCTCCATTCACGCTTGAAAGTTTGAAGGTTAGTGAGAAATTGAAGAACTTGGGGTCAAGGAACTTCTTTCTATGCTCAAAGCCTACTTCAATCTGA
Protein sequence

>Msa0096000

MGGTVSTPQTDKFPLDTTETNDSPLRPSKLLLNVSIENTLGAIQVLMSPENTVGELVKVALMTYDKEKRRPLLKDTDPNRYQLHFSPFTLESLKVSEKLKNLGSRNFFLCSKPTSI*