Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418240 | sp|Q3E6T0|PT525_ARATH | 81.686 | 344 | 63 | 0 | 2 | 345 | 6 | 349 | 0.0 | 598 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000880 | Msa1418240 | 0.817399 | 3.365134e-52 | -8.615850e-47 |
| Msa0001960 | Msa1418240 | 0.829815 | 4.167440e-55 | -8.615850e-47 |
| Msa0002680 | Msa1418240 | 0.846565 | 2.004875e-59 | -8.615850e-47 |
| Msa0002710 | Msa1418240 | 0.816789 | 4.613177e-52 | -8.615850e-47 |
| Msa0003700 | Msa1418240 | 0.808803 | 2.588027e-50 | -8.615850e-47 |
| Msa0003720 | Msa1418240 | 0.843599 | 1.269044e-58 | -8.615850e-47 |
| Msa0004540 | Msa1418240 | 0.817042 | 4.049172e-52 | -8.615850e-47 |
| Msa0007990 | Msa1418240 | 0.804647 | 1.953746e-49 | -8.615850e-47 |
| Msa0011840 | Msa1418240 | 0.804223 | 2.395199e-49 | -8.615850e-47 |
| Msa0011930 | Msa1418240 | 0.852818 | 3.595310e-61 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418240 | MtrunA17_Chr4g0063601 | 91.884 | 345 | 0 | 1 | 1 | 345 | 1 | 317 | 0.0 | 632 |
| Msa1418240 | MtrunA17_Chr3g0137921 | 84.302 | 344 | 54 | 0 | 2 | 345 | 6 | 349 | 0.0 | 610 |
| Msa1418240 | MtrunA17_Chr2g0307091 | 83.381 | 349 | 53 | 3 | 1 | 344 | 1 | 349 | 0.0 | 588 |
| Msa1418240 | MtrunA17_Chr7g0270591 | 44.940 | 336 | 181 | 3 | 9 | 343 | 10 | 342 | 2.52e-104 | 311 |
| Msa1418240 | MtrunA17_Chr4g0069261 | 50.000 | 306 | 151 | 2 | 9 | 313 | 6 | 310 | 2.05e-98 | 296 |
| Msa1418240 | MtrunA17_Chr2g0312911 | 43.302 | 321 | 179 | 3 | 13 | 331 | 10 | 329 | 1.50e-85 | 261 |
| Msa1418240 | MtrunA17_Chr3g0112981 | 32.441 | 299 | 182 | 6 | 18 | 305 | 29 | 318 | 3.46e-33 | 125 |
| Msa1418240 | MtrunA17_Chr8g0382081 | 34.007 | 297 | 180 | 9 | 18 | 305 | 16 | 305 | 1.26e-32 | 123 |
| Msa1418240 | MtrunA17_Chr5g0436341 | 32.558 | 301 | 183 | 6 | 16 | 305 | 11 | 302 | 2.83e-32 | 122 |
| Msa1418240 | MtrunA17_Chr1g0160071 | 23.684 | 342 | 244 | 7 | 3 | 332 | 1 | 337 | 7.82e-28 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418240 | AT5G25400.1 | 81.686 | 344 | 63 | 0 | 2 | 345 | 6 | 349 | 0.0 | 598 |
| Msa1418240 | AT2G25520.1 | 82.558 | 344 | 58 | 1 | 2 | 345 | 6 | 347 | 0.0 | 593 |
| Msa1418240 | AT4G32390.1 | 82.029 | 345 | 61 | 1 | 2 | 345 | 6 | 350 | 0.0 | 593 |
| Msa1418240 | AT5G11230.1 | 79.710 | 345 | 69 | 1 | 2 | 345 | 6 | 350 | 0.0 | 569 |
| Msa1418240 | AT3G17430.2 | 45.427 | 328 | 177 | 2 | 9 | 335 | 7 | 333 | 4.83e-98 | 295 |
| Msa1418240 | AT3G17430.1 | 45.427 | 328 | 177 | 2 | 9 | 335 | 7 | 333 | 4.83e-98 | 295 |
| Msa1418240 | AT1G48230.1 | 46.334 | 341 | 169 | 3 | 9 | 336 | 7 | 346 | 1.39e-96 | 291 |
| Msa1418240 | AT3G14410.1 | 42.771 | 332 | 187 | 3 | 4 | 333 | 5 | 335 | 1.32e-82 | 254 |
| Msa1418240 | AT1G53660.1 | 41.390 | 331 | 183 | 6 | 6 | 334 | 9 | 330 | 2.38e-82 | 253 |
| Msa1418240 | AT1G53660.3 | 41.390 | 331 | 183 | 6 | 6 | 334 | 27 | 348 | 4.64e-82 | 253 |
Find 64 sgRNAs with CRISPR-Local
Find 166 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGATAACGGTGAAGCTG+AGG | 0.632551 | tig0024555:-40418 | None:intergenic |
| AACAGCAACACCAAGAGAAA+TGG | 0.430794 | tig0024555:-40780 | None:intergenic |
| AACCTTCTTCACAACGCCAT+CGG | 0.572106 | tig0024555:-40360 | None:intergenic |
| AAGAGAAATGGAAATCATAT+TGG | 0.391631 | tig0024555:-40768 | None:intergenic |
| AATATGATTTCCATTTCTCT+TGG | 0.417528 | tig0024555:+40770 | Msa1418240:CDS |
| AGAGTGATGAAGAGGCTGGA+AGG | 0.603959 | tig0024555:+41308 | Msa1418240:CDS |
| AGATCGAGTTGAATCAGTTC+TGG | 0.413437 | tig0024555:-41378 | None:intergenic |
| AGATCGCGTGACATTGAAAC+AGG | 0.469171 | tig0024555:-40566 | None:intergenic |
| AGCGAGTAAAGTGCACCGAT+TGG | 0.499200 | tig0024555:-40608 | None:intergenic |
| AGGTTGTTGGAACAGAGAGA+TGG | 0.527055 | tig0024555:+41328 | Msa1418240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAGATCGTAAGATGTACAAT+TGG | + | tig0024555:40456-40475 | Msa1418240:CDS | 30.0% | |
| GGAAGCTAAATTTGATACTT+GGG | + | tig0024555:40814-40833 | Msa1418240:CDS | 30.0% | |
| GAAGCTAAATTTGATACTTG+GGG | + | tig0024555:40815-40834 | Msa1418240:CDS | 30.0% | |
| TTATAATCATTCGAAGTTGC+AGG | + | tig0024555:41249-41268 | Msa1418240:CDS | 30.0% | |
| TAGATCGTAAGATGTACAAT+TGG | + | tig0024555:40456-40475 | Msa1418240:CDS | 30.0% | |
| GGAAGCTAAATTTGATACTT+GGG | + | tig0024555:40814-40833 | Msa1418240:CDS | 30.0% | |
| GAAGCTAAATTTGATACTTG+GGG | + | tig0024555:40815-40834 | Msa1418240:CDS | 30.0% | |
| TTATAATCATTCGAAGTTGC+AGG | + | tig0024555:41249-41268 | Msa1418240:CDS | 30.0% | |
| TGAATCATGGTTAGAGAGAT+CGG | - | tig0024555:40488-40507 | None:intergenic | 35.0% | |
| ACAAAAAGCCATGTGAATCA+TGG | - | tig0024555:40501-40520 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0024555 | gene | 40350 | 41387 | 40350 | ID=Msa1418240;Name=Msa1418240 |
| tig0024555 | mRNA | 40350 | 41387 | 40350 | ID=Msa1418240-mRNA-1;Parent=Msa1418240;Name=Msa1418240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|345 |
| tig0024555 | exon | 40350 | 41387 | 40350 | ID=Msa1418240-mRNA-1:exon:234;Parent=Msa1418240-mRNA-1 |
| tig0024555 | CDS | 40350 | 41387 | 40350 | ID=Msa1418240-mRNA-1:cds;Parent=Msa1418240-mRNA-1 |
| Gene Sequence |
>Msa1418240 ATGGCGGTTTCCGATGGCGTTGTGAAGAAGGTTATCCTCTCATACACCTACGTCGCAATCTGGATCTTCCTCAGCTTCACCGTTATCGTTTACAACAAATACATCTTAGATCGTAAGATGTACAATTGGCCTTACCCGATCTCTCTAACCATGATTCACATGGCTTTTTGTTCTTCACTCGCTTACGTTCTCGTTCGTGTTTTCAAGCTTGTTGAACCTGTTTCAATGTCACGCGATCTGTATCTCAAATCTGTTGTTCCAATCGGTGCACTTTACTCGCTTTCGTTATGGTTTTCCAATTCTGCGTATATTTATCTATCTGTTTCGTTTATTCAGATGCTGAAAGCGCTTATGCCTGTTGCTGTTTATTCGATTGGTGTTTTGTTTAAGAAAGAAGGTTTTAAGAATGAAACTATGGCCAATATGATTTCCATTTCTCTTGGTGTTGCTGTTGCTGCTTATGGGGAAGCTAAATTTGATACTTGGGGTGTTACACTTCAGCTTATGGCTGTTGCTTTTGAAGCAACACGTTTGGTTTTGATTCAGATTTTGCTTAATTCGAAGGGGATTTCGTTGAATCCGATTACTTCGTTGTATTACATTGCGCCATGTTGTTTGGTGTTTTTGTCTGTTCCTTGGCTTATTGTTGAGTATCCTTCGTTGAGGGATAACTCAAGTTTCCATTTGGATTTCGCGATTTTTGGGACAAATTCGCTATGTGCTTTTGCTTTGAATCTTGCCGTGTTTTTGTTGGTTGGGAAGACGTCGGCTTTGACTATGAATGTTGCTGGGGTTGTTAAGGATTGGTTGTTGATTGCATTCTCGTGGTCGGTGATTAAGGATACTGTTACACCGATTAATTTGATTGGTTATGGGTTGGCGTTTTTGGGGGTTGCGTATTATAATCATTCGAAGTTGCAGGCTCTTAAGGCTTCCGAGACGCAGAAGAAGGCTCAACAGAGTGATGAAGAGGCTGGAAGGTTGTTGGAACAGAGAGATGGAGAAGGGACTGGAAGGAAGAGCGACAGCCAGAACTGA |
| Protein sequence |
>Msa1418240 MAVSDGVVKKVILSYTYVAIWIFLSFTVIVYNKYILDRKMYNWPYPISLTMIHMAFCSSLAYVLVRVFKLVEPVSMSRDLYLKSVVPIGALYSLSLWFSNSAYIYLSVSFIQMLKALMPVAVYSIGVLFKKEGFKNETMANMISISLGVAVAAYGEAKFDTWGVTLQLMAVAFEATRLVLIQILLNSKGISLNPITSLYYIAPCCLVFLSVPWLIVEYPSLRDNSSFHLDFAIFGTNSLCAFALNLAVFLLVGKTSALTMNVAGVVKDWLLIAFSWSVIKDTVTPINLIGYGLAFLGVAYYNHSKLQALKASETQKKAQQSDEEAGRLLEQRDGEGTGRKSDSQN* |
