AlfalfaGEDB | Msa1435760

M. sativa cultivar zhongmu-4 / Msa1435760

KEGG: -
GO: GO:0000041 GO:0003674 GO:0005215 GO:0005381 GO:0005384 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006826 GO:0006828 GO:0006873 GO:0006875 GO:0006879 GO:0006880 GO:0008150 GO:0008324 GO:0009987 GO:0010035 GO:0010038 GO:0010039 GO:0015075 GO:0015318 GO:0016020 GO:0019725 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030026 GO:0034220 GO:0034755 GO:0042221 GO:0042592 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051641 GO:0051651 GO:0051716 GO:0055065 GO:0055071 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071421 GO:0097577 GO:0098655 GO:0098660 GO:0098662 GO:0098771
PFAM: PF01988.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435760	XP_003592125.1	96.396	222	2	2	1	221	1	217	1.38e-148	422

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435760	sp\|Q9M2C0\|VITH4_ARATH	68.447	206	53	3	19	221	2	198	2.43e-99	290

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435760	G7ID02	96.396	222	2	2	1	221	1	217	6.60e-149	422

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0005350	Msa1435760	0.800965	1.125342e-48	-8.615850e-47
Msa0053900	Msa1435760	0.804572	2.025466e-49	-8.615850e-47
Msa0060600	Msa1435760	0.811236	7.745761e-51	-8.615850e-47
Msa0099730	Msa1435760	0.801821	7.513232e-49	-8.615850e-47
Msa0101060	Msa1435760	0.804611	1.987536e-49	-8.615850e-47
Msa0105340	Msa1435760	0.802246	6.145040e-49	-8.615850e-47
Msa0617830	Msa1435760	0.803392	3.563553e-49	-8.615850e-47
Msa0620640	Msa1435760	0.801743	7.794822e-49	-8.615850e-47
Msa0656270	Msa1435760	0.817915	2.573707e-52	-8.615850e-47
Msa0700070	Msa1435760	0.830477	2.872457e-55	-8.615850e-47
Msa0714340	Msa1435760	0.814507	1.487350e-51	-8.615850e-47
Msa0714380	Msa1435760	0.807260	5.513578e-50	-8.615850e-47
Msa0835730	Msa1435760	0.815017	1.146585e-51	-8.615850e-47
Msa0148360	Msa1435760	0.803012	4.271721e-49	-8.615850e-47
Msa1235630	Msa1435760	0.807891	4.050051e-50	-8.615850e-47
Msa1102180	Msa1435760	0.802701	4.952600e-49	-8.615850e-47
Msa1217810	Msa1435760	0.812884	3.386515e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435760	MtrunA17_Chr1g0202031	96.396	222	2	2	1	221	1	217	1.27e-152	422
Msa1435760	MtrunA17_Chr6g0463641	79.167	216	38	3	6	221	2	210	2.71e-107	307
Msa1435760	MtrunA17_Chr4g0050821	65.550	209	71	1	13	221	17	224	1.23e-85	253
Msa1435760	MtrunA17_Chr4g0050841	66.832	202	66	1	20	221	24	224	1.56e-85	253
Msa1435760	MtrunA17_Chr4g0050831	66.832	202	66	1	20	221	24	224	5.80e-85	251
Msa1435760	MtrunA17_Chr4g0050811	62.085	211	72	2	15	221	33	239	3.27e-84	249
Msa1435760	MtrunA17_Chr6g0479471	68.783	189	49	2	28	216	29	207	4.37e-79	236
Msa1435760	MtrunA17_Chr2g0278621	68.783	189	49	2	28	216	29	207	4.88e-79	236
Msa1435760	MtrunA17_Chr4g0050851	58.333	216	83	3	9	221	20	231	5.33e-71	216

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435760	AT3G43660.1	68.447	206	53	3	19	221	2	198	2.47e-100	290
Msa1435760	AT3G43630.1	72.549	204	42	4	18	218	2	194	8.10e-94	273
Msa1435760	AT1G21140.1	70.192	208	49	4	18	221	2	200	1.19e-93	273
Msa1435760	AT1G76800.1	71.707	205	48	1	17	221	2	196	1.20e-92	270
Msa1435760	AT3G25190.1	59.434	212	81	2	14	221	7	217	2.09e-68	209

Find 43 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATAAGGGTAGGTGTTGTTT+TGG	0.269938	tig0027769:+2482	Msa1435760:CDS
ATTGCTATGGCCATAACTTT+TGG	0.287767	tig0027769:+2606	Msa1435760:CDS
GGTTGGTTGGGTGCAGTGTT+GGG	0.338405	tig0027769:+2534	Msa1435760:CDS
TTTGGACTAACCAAGTTGAT+TGG	0.369529	tig0027769:+2624	Msa1435760:CDS
TGGTTGGTTGGGTGCAGTGT+TGG	0.376731	tig0027769:+2533	Msa1435760:CDS
TTTGGTTGTGTTTGGTTGGT+TGG	0.383193	tig0027769:+2521	Msa1435760:CDS
GCTGCTGCTTGTAAAGGATT+TGG	0.384499	tig0027769:-2387	None:intergenic
ATTCATCACTTTCGATCTCT+AGG	0.393578	tig0027769:-2074	None:intergenic
CTTGGTTAGTCCAAAAGTTA+TGG	0.395661	tig0027769:-2616	None:intergenic
CATTAAAGATTATAAGATAA+GGG	0.398036	tig0027769:+2467	Msa1435760:CDS
TTGGTTGTGTTTGGTTGGTT+GGG	0.399852	tig0027769:+2522	Msa1435760:CDS
CAGTGGTAAGGTCTTGTTTG+AGG	0.403097	tig0027769:+2565	Msa1435760:CDS
AGTGGTAAGGTCTTGTTTGA+GGG	0.418892	tig0027769:+2566	Msa1435760:CDS
TTCAACAGCATCACTAATGA+TGG	0.422094	tig0027769:+2161	Msa1435760:CDS
GTTGGGTGCAGTGTTGGGTA+AGG	0.428457	tig0027769:+2539	Msa1435760:CDS
TCTTGTTTGAGGGTCTTGCT+TGG	0.436159	tig0027769:+2576	Msa1435760:CDS
ACCAAGTTGATTGGGTCAAG+TGG	0.451627	tig0027769:+2633	Msa1435760:CDS
TTGGACTAACCAAGTTGATT+GGG	0.452121	tig0027769:+2625	Msa1435760:CDS
TGTTTGAGGGTCTTGCTTGG+AGG	0.456354	tig0027769:+2579	Msa1435760:CDS
TTGCTTTGGTTGTGTTTGGT+TGG	0.457210	tig0027769:+2517	Msa1435760:CDS
TCATTAAAGATTATAAGATA+AGG	0.464621	tig0027769:+2466	Msa1435760:CDS
GCTTGGAGGTTGGATTGCTA+TGG	0.470569	tig0027769:+2593	Msa1435760:CDS
CCCACTTGACCCAATCAACT+TGG	0.477807	tig0027769:-2634	None:intergenic
AAAAGAGACAATATTGAAAG+AGG	0.486922	tig0027769:+2321	Msa1435760:CDS
ATAAAAGCCATGATCTTGTC+GGG	0.488331	tig0027769:+2207	Msa1435760:CDS
TGAGGGTCTTGCTTGGAGGT+TGG	0.503434	tig0027769:+2583	Msa1435760:CDS
CATAAAAGCCATGATCTTGT+CGG	0.507773	tig0027769:+2206	Msa1435760:CDS
TTCATCACTTTCGATCTCTA+GGG	0.515825	tig0027769:-2073	None:intergenic
AGCAAAACCCGACAAGATCA+TGG	0.526585	tig0027769:-2214	None:intergenic
GGTGCATGTAGCATGGCAAT+AGG	0.539596	tig0027769:+2249	Msa1435760:CDS
GAAGCTGCTGCTGCTTGTAA+AGG	0.540279	tig0027769:-2393	None:intergenic
ATGAAAGATGCTGCAAGAAG+TGG	0.543716	tig0027769:-2447	None:intergenic
TGGGTAAGGCACCAGTGGTA+AGG	0.548709	tig0027769:+2553	Msa1435760:CDS
AAGAGGTAATAATATTGAAG+AGG	0.573020	tig0027769:+2338	Msa1435760:CDS
AAAGATTATAAGATAAGGGT+AGG	0.576224	tig0027769:+2471	Msa1435760:CDS
CCAAGTTGATTGGGTCAAGT+GGG	0.582206	tig0027769:+2634	Msa1435760:CDS
ATCACAATGGCTACGTGCCG+CGG	0.590039	tig0027769:+2113	Msa1435760:CDS
GCATCACTAATGATGGGTGT+AGG	0.595742	tig0027769:+2168	Msa1435760:CDS
CTCAAACAAGACCTTACCAC+TGG	0.603707	tig0027769:-2564	None:intergenic
ATCATTAGCTCCTAAAACCG+CGG	0.609497	tig0027769:-2130	None:intergenic
TCAACAGCATCACTAATGAT+GGG	0.621364	tig0027769:+2162	Msa1435760:CDS
AGTGTTGGGTAAGGCACCAG+TGG	0.645814	tig0027769:+2548	Msa1435760:CDS
TGTAGCTGGTGCATGTAGCA+TGG	0.650697	tig0027769:+2242	Msa1435760:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCATTAAAGATTATAAGATA+AGG	+	tig0027769:2466-2485	Msa1435760:CDS	15.0%
!!	CATTAAAGATTATAAGATAA+GGG	+	tig0027769:2467-2486	Msa1435760:CDS	15.0%
!!!	TACAAATTTTGTTTGGTTAA+GGG	-	tig0027769:2019-2038	None:intergenic	20.0%
!!!	TTTGATGTACAAATTTTGTT+TGG	-	tig0027769:2026-2045	None:intergenic	20.0%
!!!	GTACAAATTTTGTTTGGTTA+AGG	-	tig0027769:2020-2039	None:intergenic	25.0%
!	ATTACTCAAAAAGATCACAA+TGG	+	tig0027769:2100-2119	Msa1435760:CDS	25.0%
!	AAAAGAGACAATATTGAAAG+AGG	+	tig0027769:2321-2340	Msa1435760:CDS	25.0%
!	AAGAGGTAATAATATTGAAG+AGG	+	tig0027769:2338-2357	Msa1435760:CDS	25.0%
!	AAAGATTATAAGATAAGGGT+AGG	+	tig0027769:2471-2490	Msa1435760:CDS	25.0%
!!	TCAGCTTTAGCATTTTCAAT+TGG	+	tig0027769:2414-2433	Msa1435760:CDS	30.0%
	TTCATCACTTTCGATCTCTA+GGG	-	tig0027769:2076-2095	None:intergenic	35.0%
	ATTCATCACTTTCGATCTCT+AGG	-	tig0027769:2077-2096	None:intergenic	35.0%
	TTCAACAGCATCACTAATGA+TGG	+	tig0027769:2161-2180	Msa1435760:CDS	35.0%
	TCAACAGCATCACTAATGAT+GGG	+	tig0027769:2162-2181	Msa1435760:CDS	35.0%
	CATAAAAGCCATGATCTTGT+CGG	+	tig0027769:2206-2225	Msa1435760:CDS	35.0%
	ATAAAAGCCATGATCTTGTC+GGG	+	tig0027769:2207-2226	Msa1435760:CDS	35.0%
!	AGCATTTTCAATTGGTGCAA+TGG	+	tig0027769:2422-2441	Msa1435760:CDS	35.0%
!!!	ATAAGGGTAGGTGTTGTTTT+GGG	+	tig0027769:2483-2502	Msa1435760:CDS	35.0%
!	AGTTTTGCTTTGGTTGTGTT+TGG	+	tig0027769:2513-2532	Msa1435760:CDS	35.0%
!	ATTGCTATGGCCATAACTTT+TGG	+	tig0027769:2606-2625	Msa1435760:CDS	35.0%
!	CTTGGTTAGTCCAAAAGTTA+TGG	-	tig0027769:2619-2638	None:intergenic	35.0%
!!	TTTGGACTAACCAAGTTGAT+TGG	+	tig0027769:2624-2643	Msa1435760:CDS	35.0%
!!	TTGGACTAACCAAGTTGATT+GGG	+	tig0027769:2625-2644	Msa1435760:CDS	35.0%
!	GGGTTGCATTTTGTTGATCA+TGG	-	tig0027769:2056-2075	None:intergenic	40.0%
	ATCATTAGCTCCTAAAACCG+CGG	-	tig0027769:2133-2152	None:intergenic	40.0%
	ATGAAAGATGCTGCAAGAAG+TGG	-	tig0027769:2450-2469	None:intergenic	40.0%
!!!	GATAAGGGTAGGTGTTGTTT+TGG	+	tig0027769:2482-2501	Msa1435760:CDS	40.0%
	TTGCTTTGGTTGTGTTTGGT+TGG	+	tig0027769:2517-2536	Msa1435760:CDS	40.0%
	TTTGGTTGTGTTTGGTTGGT+TGG	+	tig0027769:2521-2540	Msa1435760:CDS	40.0%
!	TTGGTTGTGTTTGGTTGGTT+GGG	+	tig0027769:2522-2541	Msa1435760:CDS	40.0%
!!	AGTGGTAAGGTCTTGTTTGA+GGG	+	tig0027769:2566-2585	Msa1435760:CDS	40.0%
!!!	GCGGTTTTAGGAGCTAATGA+TGG	+	tig0027769:2132-2151	Msa1435760:CDS	45.0%
!!	GCATCACTAATGATGGGTGT+AGG	+	tig0027769:2168-2187	Msa1435760:CDS	45.0%
	AGCAAAACCCGACAAGATCA+TGG	-	tig0027769:2217-2236	None:intergenic	45.0%
!	ATGATCTTGTCGGGTTTTGC+TGG	+	tig0027769:2216-2235	Msa1435760:CDS	45.0%
!	GCTGCTGCTTGTAAAGGATT+TGG	-	tig0027769:2390-2409	None:intergenic	45.0%
!!!	GGGAGCAGTTAGTTTTGCTT+TGG	+	tig0027769:2503-2522	Msa1435760:CDS	45.0%
	CTCAAACAAGACCTTACCAC+TGG	-	tig0027769:2567-2586	None:intergenic	45.0%
!!	CAGTGGTAAGGTCTTGTTTG+AGG	+	tig0027769:2565-2584	Msa1435760:CDS	45.0%
!	TCTTGTTTGAGGGTCTTGCT+TGG	+	tig0027769:2576-2595	Msa1435760:CDS	45.0%
!!	ACCAAGTTGATTGGGTCAAG+TGG	+	tig0027769:2633-2652	Msa1435760:CDS	45.0%
!!	CCAAGTTGATTGGGTCAAGT+GGG	+	tig0027769:2634-2653	Msa1435760:CDS	45.0%
!	GGTTTTGCTGGACTTGTAGC+TGG	+	tig0027769:2228-2247	Msa1435760:CDS	50.0%
	TGTAGCTGGTGCATGTAGCA+TGG	+	tig0027769:2242-2261	Msa1435760:CDS	50.0%
	GGTGCATGTAGCATGGCAAT+AGG	+	tig0027769:2249-2268	Msa1435760:CDS	50.0%
!	GAAGCTGCTGCTGCTTGTAA+AGG	-	tig0027769:2396-2415	None:intergenic	50.0%
	TGTTTGAGGGTCTTGCTTGG+AGG	+	tig0027769:2579-2598	Msa1435760:CDS	50.0%
	GCTTGGAGGTTGGATTGCTA+TGG	+	tig0027769:2593-2612	Msa1435760:CDS	50.0%
	CCCACTTGACCCAATCAACT+TGG	-	tig0027769:2637-2656	None:intergenic	50.0%
	ATCACAATGGCTACGTGCCG+CGG	+	tig0027769:2113-2132	Msa1435760:CDS	55.0%
!	TGGTTGGTTGGGTGCAGTGT+TGG	+	tig0027769:2533-2552	Msa1435760:CDS	55.0%
!	GGTTGGTTGGGTGCAGTGTT+GGG	+	tig0027769:2534-2553	Msa1435760:CDS	55.0%
!	GTTGGGTGCAGTGTTGGGTA+AGG	+	tig0027769:2539-2558	Msa1435760:CDS	55.0%
!	AGTGTTGGGTAAGGCACCAG+TGG	+	tig0027769:2548-2567	Msa1435760:CDS	55.0%
!	TGGGTAAGGCACCAGTGGTA+AGG	+	tig0027769:2553-2572	Msa1435760:CDS	55.0%
	TGAGGGTCTTGCTTGGAGGT+TGG	+	tig0027769:2583-2602	Msa1435760:CDS	55.0%
!	TGGCTACGTGCCGCGGTTTT+AGG	+	tig0027769:2120-2139	Msa1435760:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0027769	gene	1997	2662	1997	ID=Msa1435760;Name=Msa1435760
tig0027769	mRNA	1997	2662	1997	ID=Msa1435760-mRNA-1;Parent=Msa1435760;Name=Msa1435760-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|221
tig0027769	exon	1997	2662	1997	ID=Msa1435760-mRNA-1:exon:9867;Parent=Msa1435760-mRNA-1
tig0027769	CDS	1997	2662	1997	ID=Msa1435760-mRNA-1:cds;Parent=Msa1435760-mRNA-1

Gene Sequence

>Msa1435760

ATGGCATCTAATAATTCATCCCTTAACCAAACAAAATTTGTACATCAAAACAATGACCATGATCAACAAAATGCAACCCTAGAGATCGAAAGTGATGAATTTGATTACTCAAAAAGATCACAATGGCTACGTGCCGCGGTTTTAGGAGCTAATGATGGATTAGTTTCAACAGCATCACTAATGATGGGTGTAGGAGCAGTGAAACAAGACATAAAAGCCATGATCTTGTCGGGTTTTGCTGGACTTGTAGCTGGTGCATGTAGCATGGCAATAGGTGAGTTTGTATCTGTTTACTCACAACTTGACATTGAAATTGCTCAATTAAAAAGAGACAATATTGAAAGAGGTAATAATATTGAAGAGGAAGATGTCATTGAGAAAGAAAGTTTGCCAAATCCTTTACAAGCAGCAGCAGCTTCAGCTTTAGCATTTTCAATTGGTGCAATGGTGCCACTTCTTGCAGCATCTTTCATTAAAGATTATAAGATAAGGGTAGGTGTTGTTTTGGGAGCAGTTAGTTTTGCTTTGGTTGTGTTTGGTTGGTTGGGTGCAGTGTTGGGTAAGGCACCAGTGGTAAGGTCTTGTTTGAGGGTCTTGCTTGGAGGTTGGATTGCTATGGCCATAACTTTTGGACTAACCAAGTTGATTGGGTCAAGTGGGCTATAG

Protein sequence

>Msa1435760

MASNNSSLNQTKFVHQNNDHDQQNATLEIESDEFDYSKRSQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILSGFAGLVAGACSMAIGEFVSVYSQLDIEIAQLKRDNIERGNNIEEEDVIEKESLPNPLQAAAASALAFSIGAMVPLLAASFIKDYKIRVGVVLGAVSFALVVFGWLGAVLGKAPVVRSCLRVLLGGWIAMAITFGLTKLIGSSGL*