Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1456000 | XP_003595045.1 | 99.454 | 183 | 1 | 0 | 1 | 183 | 1 | 183 | 5.30e-131 | 375 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1456000 | Q2HUM6 | 99.454 | 183 | 1 | 0 | 1 | 183 | 1 | 183 | 2.53e-131 | 375 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0051960 | Msa1456000 | 0.825756 | 3.940467e-54 | -8.615850e-47 |
| Msa0089890 | Msa1456000 | 0.829764 | 4.289515e-55 | -8.615850e-47 |
| Msa0101960 | Msa1456000 | 0.820634 | 6.183300e-53 | -8.615850e-47 |
| Msa1351230 | Msa1456000 | 0.842449 | 2.569184e-58 | -8.615850e-47 |
| Msa1395250 | Msa1456000 | 0.804194 | 2.428635e-49 | -8.615850e-47 |
| Msa1435320 | Msa1456000 | 0.831681 | 1.454666e-55 | -8.615850e-47 |
| Msa1443400 | Msa1456000 | 0.835571 | 1.553008e-56 | -8.615850e-47 |
| Msa1447880 | Msa1456000 | 0.800852 | 1.186431e-48 | -8.615850e-47 |
| Msa1448040 | Msa1456000 | 0.832074 | 1.163214e-55 | -8.615850e-47 |
| Msa0650560 | Msa1456000 | 0.841715 | 4.015785e-58 | -8.615850e-47 |
| Msa0670170 | Msa1456000 | 0.822951 | 1.799812e-53 | -8.615850e-47 |
| Msa0717860 | Msa1456000 | 0.801770 | 7.699023e-49 | -8.615850e-47 |
| Msa0740630 | Msa1456000 | 0.811677 | 6.211535e-51 | -8.615850e-47 |
| Msa0746340 | Msa1456000 | 0.804967 | 1.675053e-49 | -8.615850e-47 |
| Msa0753930 | Msa1456000 | 0.801370 | 9.296225e-49 | -8.615850e-47 |
| Msa0788660 | Msa1456000 | 0.800541 | 1.373291e-48 | -8.615850e-47 |
| Msa0804870 | Msa1456000 | 0.833085 | 6.528936e-56 | -8.615850e-47 |
| Msa0820880 | Msa1456000 | 0.811097 | 8.299719e-51 | -8.615850e-47 |
| Msa0832950 | Msa1456000 | 0.824954 | 6.099566e-54 | -8.615850e-47 |
| Msa0838870 | Msa1456000 | 0.808813 | 2.575470e-50 | -8.615850e-47 |
| Msa0136240 | Msa1456000 | 0.834638 | 2.669293e-56 | -8.615850e-47 |
| Msa0230080 | Msa1456000 | 0.845182 | 4.762770e-59 | -8.615850e-47 |
| Msa1336300 | Msa1456000 | 0.809428 | 1.901087e-50 | -8.615850e-47 |
| Msa0387040 | Msa1456000 | 0.811468 | 6.896936e-51 | -8.615850e-47 |
| Msa0425590 | Msa1456000 | 0.850141 | 2.056604e-60 | -8.615850e-47 |
| Msa0472090 | Msa1456000 | 0.848315 | 6.627734e-60 | -8.615850e-47 |
| Msa1082520 | Msa1456000 | 0.811497 | 6.797706e-51 | -8.615850e-47 |
| Msa1084890 | Msa1456000 | 0.811273 | 7.601149e-51 | -8.615850e-47 |
| Msa0296910 | Msa1456000 | 0.800397 | 1.469020e-48 | -8.615850e-47 |
| Msa0306310 | Msa1456000 | 0.833778 | 4.386884e-56 | -8.615850e-47 |
| Msa0335070 | Msa1456000 | 0.808617 | 2.835698e-50 | -8.615850e-47 |
| Msa0516740 | Msa1456000 | 0.826325 | 2.885898e-54 | -8.615850e-47 |
| Msa0599220 | Msa1456000 | 0.813477 | 2.508939e-51 | -8.615850e-47 |
| Msa0859040 | Msa1456000 | 0.823977 | 1.035597e-53 | -8.615850e-47 |
| Msa0923070 | Msa1456000 | 0.814069 | 1.858324e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1456000 | MtrunA17_Chr2g0298551 | 99.454 | 183 | 1 | 0 | 1 | 183 | 1 | 183 | 4.87e-135 | 375 |
| Msa1456000 | MtrunA17_Chr4g0071901 | 58.378 | 185 | 69 | 4 | 1 | 182 | 44 | 223 | 1.97e-66 | 202 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCACTTGGTTAACTGAAAT+TGG | 0.230756 | tig0036810:-7525 | None:intergenic |
| AAGATGCTTTGAGCAGTATT+TGG | 0.233848 | tig0036810:+7799 | Msa1456000:CDS |
| CGATGTTTCACCTGTGGATT+TGG | 0.275973 | tig0036810:+7686 | Msa1456000:CDS |
| AAAGCATCTTGGTTTGATAA+AGG | 0.331144 | tig0036810:-7786 | None:intergenic |
| GCACATGTAGAGGGCGGTTA+TGG | 0.354136 | tig0036810:+7892 | Msa1456000:CDS |
| TCGGCTTGTAGAATTGAATA+AGG | 0.390315 | tig0036810:-7666 | None:intergenic |
| AGCATGAAGTAAACATTTCC+TGG | 0.401282 | tig0036810:-7642 | None:intergenic |
| TTCGGCCACTGCAACTCTGC+CGG | 0.411739 | tig0036810:-7841 | None:intergenic |
| CACATCAAAGAAAATCCGTT+TGG | 0.427399 | tig0036810:+7467 | Msa1456000:CDS |
| GCGGTTATGGAGACCTCTAT+TGG | 0.447733 | tig0036810:+7905 | Msa1456000:CDS |
| ATCCGTTTGGTTCATCTAAG+TGG | 0.450947 | tig0036810:+7480 | Msa1456000:CDS |
| AAATACTGCTCAAAGCATCT+TGG | 0.454917 | tig0036810:-7797 | None:intergenic |
| AGTTGTGTATCTCCTTTCAA+TGG | 0.456894 | tig0036810:-7615 | None:intergenic |
| TCATTGATATTCTTTCAAAT+CGG | 0.458020 | tig0036810:-7967 | None:intergenic |
| AAGAAGATGAAAGAAGTTGA+AGG | 0.463012 | tig0036810:-7587 | None:intergenic |
| GATTTCTACTATTGATCACT+TGG | 0.465741 | tig0036810:-7539 | None:intergenic |
| TCGGCCACTGCAACTCTGCC+GGG | 0.472425 | tig0036810:-7840 | None:intergenic |
| ATTTGGCTTCGCTTGCGAAA+AGG | 0.473956 | tig0036810:+7703 | Msa1456000:CDS |
| TGCGAAAAGGCTCACTGCAA+AGG | 0.520433 | tig0036810:+7716 | Msa1456000:CDS |
| TCTTCTTCAAAACCATTGAA+AGG | 0.529265 | tig0036810:+7603 | Msa1456000:CDS |
| ATACGTCATACCGTATTGTT+CGG | 0.544090 | tig0036810:-7859 | None:intergenic |
| GAACAATACGGTATGACGTA+TGG | 0.550185 | tig0036810:+7861 | Msa1456000:CDS |
| AGAAGATGAAAGAAGTTGAA+GGG | 0.551501 | tig0036810:-7586 | None:intergenic |
| AGTTCGCCTTCGATGAGTCC+CGG | 0.573481 | tig0036810:+7822 | Msa1456000:CDS |
| GTTGCAGTGGCCGAACAATA+CGG | 0.575627 | tig0036810:+7849 | Msa1456000:CDS |
| ACAAGCCGATGTTTCACCTG+TGG | 0.577221 | tig0036810:+7680 | Msa1456000:CDS |
| ACTCTGCCGGGACTCATCGA+AGG | 0.577322 | tig0036810:-7828 | None:intergenic |
| GAGTCCCGGCAGAGTTGCAG+TGG | 0.594628 | tig0036810:+7836 | Msa1456000:CDS |
| TCTCTGATAGTATCCAATAG+AGG | 0.601725 | tig0036810:-7918 | None:intergenic |
| AACCACTTAGATGAACCAAA+CGG | 0.602554 | tig0036810:-7482 | None:intergenic |
| GTACAAACAAAGTGAGTAGG+TGG | 0.603098 | tig0036810:-7561 | None:intergenic |
| AGATGAAAGAAGTTGAAGGG+AGG | 0.610782 | tig0036810:-7583 | None:intergenic |
| GAGGTACAAACAAAGTGAGT+AGG | 0.613139 | tig0036810:-7564 | None:intergenic |
| GCAAGCGAAGCCAAATCCAC+AGG | 0.614717 | tig0036810:-7696 | None:intergenic |
| CAAATCCACAGGTGAAACAT+CGG | 0.618683 | tig0036810:-7685 | None:intergenic |
| GGAGGACGAAGCACATGTAG+AGG | 0.624245 | tig0036810:+7882 | Msa1456000:CDS |
| GAGGACGAAGCACATGTAGA+GGG | 0.636486 | tig0036810:+7883 | Msa1456000:CDS |
| CAATACGGTATGACGTATGG+AGG | 0.653418 | tig0036810:+7864 | Msa1456000:CDS |
| GACGAAGCACATGTAGAGGG+CGG | 0.686792 | tig0036810:+7886 | Msa1456000:CDS |
| GGAGATACACAACTTCAACC+AGG | 0.693853 | tig0036810:+7624 | Msa1456000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATTGATATTCTTTCAAAT+CGG | - | tig0036810:7970-7989 | None:intergenic | 20.0% |
| ATCACTTGGTTAACTGAAAT+TGG | - | tig0036810:7528-7547 | None:intergenic | 30.0% | |
| GATTTCTACTATTGATCACT+TGG | - | tig0036810:7542-7561 | None:intergenic | 30.0% | |
| AGAAGATGAAAGAAGTTGAA+GGG | - | tig0036810:7589-7608 | None:intergenic | 30.0% | |
| AAGAAGATGAAAGAAGTTGA+AGG | - | tig0036810:7590-7609 | None:intergenic | 30.0% | |
| TCTTCTTCAAAACCATTGAA+AGG | + | tig0036810:7603-7622 | Msa1456000:CDS | 30.0% | |
| GATGAGAAAAAGTCATTGAA+AGG | + | tig0036810:7756-7775 | Msa1456000:CDS | 30.0% | |
| ! | AAAGCATCTTGGTTTGATAA+AGG | - | tig0036810:7789-7808 | None:intergenic | 30.0% |
| CACATCAAAGAAAATCCGTT+TGG | + | tig0036810:7467-7486 | Msa1456000:CDS | 35.0% | |
| AACCACTTAGATGAACCAAA+CGG | - | tig0036810:7485-7504 | None:intergenic | 35.0% | |
| AGTTGTGTATCTCCTTTCAA+TGG | - | tig0036810:7618-7637 | None:intergenic | 35.0% | |
| AGCATGAAGTAAACATTTCC+TGG | - | tig0036810:7645-7664 | None:intergenic | 35.0% | |
| ! | TCGGCTTGTAGAATTGAATA+AGG | - | tig0036810:7669-7688 | None:intergenic | 35.0% |
| ! | AAATACTGCTCAAAGCATCT+TGG | - | tig0036810:7800-7819 | None:intergenic | 35.0% |
| ! | AAGATGCTTTGAGCAGTATT+TGG | + | tig0036810:7799-7818 | Msa1456000:CDS | 35.0% |
| ATACGTCATACCGTATTGTT+CGG | - | tig0036810:7862-7881 | None:intergenic | 35.0% | |
| TCTCTGATAGTATCCAATAG+AGG | - | tig0036810:7921-7940 | None:intergenic | 35.0% | |
| ! | ATCCGTTTGGTTCATCTAAG+TGG | + | tig0036810:7480-7499 | Msa1456000:CDS | 40.0% |
| GTACAAACAAAGTGAGTAGG+TGG | - | tig0036810:7564-7583 | None:intergenic | 40.0% | |
| GAGGTACAAACAAAGTGAGT+AGG | - | tig0036810:7567-7586 | None:intergenic | 40.0% | |
| AGATGAAAGAAGTTGAAGGG+AGG | - | tig0036810:7586-7605 | None:intergenic | 40.0% | |
| CAAATCCACAGGTGAAACAT+CGG | - | tig0036810:7688-7707 | None:intergenic | 40.0% | |
| GAACAATACGGTATGACGTA+TGG | + | tig0036810:7861-7880 | Msa1456000:CDS | 40.0% | |
| GGAGATACACAACTTCAACC+AGG | + | tig0036810:7624-7643 | Msa1456000:CDS | 45.0% | |
| CGATGTTTCACCTGTGGATT+TGG | + | tig0036810:7686-7705 | Msa1456000:CDS | 45.0% | |
| ! | ATTTGGCTTCGCTTGCGAAA+AGG | + | tig0036810:7703-7722 | Msa1456000:CDS | 45.0% |
| CAATACGGTATGACGTATGG+AGG | + | tig0036810:7864-7883 | Msa1456000:CDS | 45.0% | |
| ACAAGCCGATGTTTCACCTG+TGG | + | tig0036810:7680-7699 | Msa1456000:CDS | 50.0% | |
| TGCGAAAAGGCTCACTGCAA+AGG | + | tig0036810:7716-7735 | Msa1456000:CDS | 50.0% | |
| GTTGCAGTGGCCGAACAATA+CGG | + | tig0036810:7849-7868 | Msa1456000:CDS | 50.0% | |
| GAGGACGAAGCACATGTAGA+GGG | + | tig0036810:7883-7902 | Msa1456000:CDS | 50.0% | |
| GCGGTTATGGAGACCTCTAT+TGG | + | tig0036810:7905-7924 | Msa1456000:CDS | 50.0% | |
| GCAAGCGAAGCCAAATCCAC+AGG | - | tig0036810:7699-7718 | None:intergenic | 55.0% | |
| AGTTCGCCTTCGATGAGTCC+CGG | + | tig0036810:7822-7841 | Msa1456000:CDS | 55.0% | |
| GGAGGACGAAGCACATGTAG+AGG | + | tig0036810:7882-7901 | Msa1456000:CDS | 55.0% | |
| GACGAAGCACATGTAGAGGG+CGG | + | tig0036810:7886-7905 | Msa1456000:CDS | 55.0% | |
| GCACATGTAGAGGGCGGTTA+TGG | + | tig0036810:7892-7911 | Msa1456000:CDS | 55.0% | |
| ACTCTGCCGGGACTCATCGA+AGG | - | tig0036810:7831-7850 | None:intergenic | 60.0% | |
| TTCGGCCACTGCAACTCTGC+CGG | - | tig0036810:7844-7863 | None:intergenic | 60.0% | |
| GAGTCCCGGCAGAGTTGCAG+TGG | + | tig0036810:7836-7855 | Msa1456000:CDS | 65.0% | |
| TCGGCCACTGCAACTCTGCC+GGG | - | tig0036810:7843-7862 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0036810 | gene | 7435 | 7989 | 7435 | ID=Msa1456000;Name=Msa1456000 |
| tig0036810 | mRNA | 7435 | 7989 | 7435 | ID=Msa1456000-mRNA-1;Parent=Msa1456000;Name=Msa1456000-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|184 |
| tig0036810 | exon | 7435 | 7989 | 7435 | ID=Msa1456000-mRNA-1:exon:20520;Parent=Msa1456000-mRNA-1 |
| tig0036810 | CDS | 7435 | 7989 | 7435 | ID=Msa1456000-mRNA-1:cds;Parent=Msa1456000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
