AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002675

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002675.t1 MTR_2g018260 94.690 226 11 1 1 226 1 225 4.97e-161 444
MS.gene002675.t1 MTR_1g045130 54.237 177 78 2 51 226 31 205 7.95e-66 202
MS.gene002675.t1 MTR_6g029230 35.849 159 100 1 41 199 40 196 4.60e-34 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002675.t1 AT4G18040 63.750 240 68 2 1 226 1 235 2.36e-107 309
MS.gene002675.t1 AT1G29550 64.894 188 66 0 39 226 53 240 1.90e-92 271
MS.gene002675.t1 AT1G29590 64.362 188 67 0 39 226 53 240 7.12e-92 270
MS.gene002675.t1 AT5G35620 49.457 184 85 5 38 215 12 193 5.49e-57 179
MS.gene002675.t1 AT5G35620 49.457 184 85 5 38 215 12 193 5.49e-57 179
MS.gene002675.t1 AT5G35620 47.879 165 78 5 31 189 5 167 5.23e-47 153
MS.gene002675.t1 AT5G18110 32.487 197 118 2 4 199 9 191 4.65e-30 111

Find 52 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAATTCTTGGACTTTC+TGG 0.032991 2.1:+68397818 MS.gene002675:CDS
TGATCGTCGTTGATGGATTT+TGG 0.121670 2.1:-68397679 None:intergenic
CAAATAGGGTCTTCCCATTT+TGG 0.162249 2.1:-68398940 None:intergenic
CTCACCCTCTTGAGAATTCT+TGG 0.186379 2.1:+68397809 MS.gene002675:CDS
AGCATAAGATTGAACCAAAA+TGG 0.214116 2.1:+68398926 MS.gene002675:CDS
TTTCTACTGTTGAAGAGTTT+TGG 0.246017 2.1:+68397908 MS.gene002675:CDS
TTTCCACCATTAGCACAAAT+AGG 0.313249 2.1:-68398955 None:intergenic
GTGACCGGCGGTGAGGGGTT+TGG 0.329982 2.1:-68397782 None:intergenic
ACTACCCCAAGCTTGACTTT+TGG 0.352217 2.1:-68397863 None:intergenic
CTTTAGTTGTTGGCAATGAT+TGG 0.357757 2.1:+68399129 MS.gene002675:intron
TTCCACCATTAGCACAAATA+GGG 0.371291 2.1:-68398954 None:intergenic
AGATTGGTGACCGGCGGTGA+GGG 0.372856 2.1:-68397788 None:intergenic
TGTGATTTGATCGTCGTTGA+TGG 0.385098 2.1:-68397686 None:intergenic
CAGTTGTGAATGTCAGGAAT+AGG 0.393268 2.1:+68399187 MS.gene002675:CDS
GGCGGTGAGGGGTTTGGAAA+TGG 0.408337 2.1:-68397776 None:intergenic
TAGATCCAAAAGTCAAGCTT+GGG 0.410080 2.1:+68397858 MS.gene002675:CDS
TGATACTAGCTGGTTGTATA+CGG 0.414513 2.1:+68399008 MS.gene002675:CDS
TTTGTGCTAATGGTGGAAAA+TGG 0.417291 2.1:+68398959 MS.gene002675:CDS
AATTCTCAAGAGGGTGAGAT+TGG 0.439429 2.1:-68397804 None:intergenic
TGCTGCTAATGAAGCTGCTC+AGG 0.454593 2.1:+68399239 MS.gene002675:CDS
CTTGATTATAATGAAACCAT+TGG 0.456939 2.1:+68400231 MS.gene002675:CDS
GCATAAGATTGAACCAAAAT+GGG 0.459310 2.1:+68398927 MS.gene002675:CDS
CAATCTTATGCTTGAAACAA+TGG 0.479043 2.1:-68398915 None:intergenic
GATCATGGAGATGAAATTTG+TGG 0.479695 2.1:+68399162 MS.gene002675:CDS
ATTGGAGAGCAATTTGATCA+TGG 0.480040 2.1:+68399147 MS.gene002675:CDS
AGTTGTGAATGTCAGGAATA+GGG 0.485043 2.1:+68399188 MS.gene002675:CDS
GCTCCAACAGCCGACTTGCT+AGG 0.494752 2.1:-68398886 None:intergenic
GTGGAGCAGTTGTGAATGTC+AGG 0.501928 2.1:+68399181 MS.gene002675:CDS
GAAGACCCTATTTGTGCTAA+TGG 0.505377 2.1:+68398949 MS.gene002675:CDS
CTAGATCCAAAAGTCAAGCT+TGG 0.513357 2.1:+68397857 MS.gene002675:CDS
GGTGAGGGGTTTGGAAATGG+CGG 0.516008 2.1:-68397773 None:intergenic
AACAGTAGAAAGAGTGTAGA+CGG 0.531049 2.1:-68397896 None:intergenic
CATCCTAGCAAGTCGGCTGT+TGG 0.531545 2.1:+68398883 MS.gene002675:CDS
GGGGAAAATCTGATACTAGC+TGG 0.537034 2.1:+68398998 MS.gene002675:CDS
CAACAGCCGACTTGCTAGGA+TGG 0.540885 2.1:-68398882 None:intergenic
GTTATATTTCAGGTGAGCAT+CGG 0.549012 2.1:+68400189 MS.gene002675:intron
GATTGGTGACCGGCGGTGAG+GGG 0.563209 2.1:-68397787 None:intergenic
GAGATTGGTGACCGGCGGTG+AGG 0.563789 2.1:-68397789 None:intergenic
ATTTCCAAACCCCTCACCGC+CGG 0.575370 2.1:+68397778 MS.gene002675:CDS
TACATGGAATATAAAGCCAA+TGG 0.575752 2.1:-68400247 None:intergenic
GAGGGGTTTGGAAATGGCGG+AGG 0.595654 2.1:-68397770 None:intergenic
GAGCATCGGAAAACAGTGGA+AGG 0.603606 2.1:+68400203 MS.gene002675:CDS
GACCCTATTTGTGCTAATGG+TGG 0.605399 2.1:+68398952 MS.gene002675:CDS
AAGAGGGTGAGATTGGTGAC+CGG 0.612893 2.1:-68397797 None:intergenic
ACAGTAGAAAGAGTGTAGAC+GGG 0.636590 2.1:-68397895 None:intergenic
AAAGTCCAAGAATTCTCAAG+AGG 0.638231 2.1:-68397814 None:intergenic
AAGTCCAAGAATTCTCAAGA+GGG 0.640086 2.1:-68397813 None:intergenic
CATTCACCATCCTAGCAAGT+CGG 0.648679 2.1:+68398876 MS.gene002675:CDS
AGGGTGAGATTGGTGACCGG+CGG 0.679762 2.1:-68397794 None:intergenic
TGTGAATGTCAGGAATAGGG+TGG 0.682592 2.1:+68399191 MS.gene002675:CDS
AGGTGAGCATCGGAAAACAG+TGG 0.685728 2.1:+68400199 MS.gene002675:intron
AGATCCAAAAGTCAAGCTTG+GGG 0.722448 2.1:+68397859 MS.gene002675:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATGTTGAAGAATTTTTT+AGG + chr2.1:68398133-68398152 MS.gene002675:intron 15.0%
!!! AAATTAAGTTTTAAAATTGC+TGG - chr2.1:68399894-68399913 None:intergenic 15.0%
!!! AAGATTCATTTTTTATTTCT+TGG + chr2.1:68398007-68398026 MS.gene002675:intron 15.0%
!!! AATTAAGTTTTAAAATTGCT+GGG - chr2.1:68399893-68399912 None:intergenic 15.0%
!!! AGATTCATTTTTTATTTCTT+GGG + chr2.1:68398008-68398027 MS.gene002675:intron 15.0%
!!! TGATTCATTTTTAGTTAATT+GGG + chr2.1:68398096-68398115 MS.gene002675:intron 15.0%
!! AAGTTCAAGAGTAAATTATA+TGG - chr2.1:68400049-68400068 None:intergenic 20.0%
!! ATAAACTTCTGTTATATTTC+AGG + chr2.1:68400179-68400198 MS.gene002675:intron 20.0%
!! ATTTCTATAAGCTATATTTG+AGG + chr2.1:68398333-68398352 MS.gene002675:intron 20.0%
!! CTATCAAAAAATAGCTTATA+TGG - chr2.1:68399950-68399969 None:intergenic 20.0%
!! GAATTAGATGGTTATTATAA+TGG + chr2.1:68399381-68399400 MS.gene002675:intron 20.0%
!! GATTGAGAATTTAAATTTCA+GGG + chr2.1:68399824-68399843 MS.gene002675:intron 20.0%
!! TGATTGAGAATTTAAATTTC+AGG + chr2.1:68399823-68399842 MS.gene002675:intron 20.0%
!!! AAGATTCAGTTTTTATGAAT+TGG + chr2.1:68398435-68398454 MS.gene002675:intron 20.0%
!!! AATTCTATCTTTTTCACTTT+TGG + chr2.1:68397938-68397957 MS.gene002675:intron 20.0%
!!! AATTGGGTATTTTTTACATT+AGG + chr2.1:68398068-68398087 MS.gene002675:intron 20.0%
!!! AATTTAGTCTGAAATTATCA+TGG + chr2.1:68399789-68399808 MS.gene002675:intron 20.0%
!!! ACAATGTTGTAGAATTTTTT+AGG + chr2.1:68398517-68398536 MS.gene002675:intron 20.0%
!!! AGATTCAGTTTTTATGAATT+GGG + chr2.1:68398436-68398455 MS.gene002675:intron 20.0%
!!! ATTGGGTATTTTTTACATTA+GGG + chr2.1:68398069-68398088 MS.gene002675:intron 20.0%
!!! ATTGTTCATTTATTGATTCT+TGG + chr2.1:68398788-68398807 MS.gene002675:intron 20.0%
!!! CTATTGAATTTGTTTTATGA+GGG + chr2.1:68399035-68399054 MS.gene002675:intron 20.0%
!!! CTTGATTATTTTTGTATCTT+GGG + chr2.1:68398681-68398700 MS.gene002675:intron 20.0%
!!! GTGATTCATTTTTAGTTAAT+TGG + chr2.1:68398095-68398114 MS.gene002675:intron 20.0%
!!! TCTTGATTATTTTTGTATCT+TGG + chr2.1:68398680-68398699 MS.gene002675:intron 20.0%
!!! TGATGAATTAAAGTACTAAT+TGG + chr2.1:68399079-68399098 MS.gene002675:intron 20.0%
!!! TTTTCAACTTTAGATCTTTT+GGG + chr2.1:68398754-68398773 MS.gene002675:intron 20.0%
! AACCATCTAATTCTTTCTTT+GGG - chr2.1:68399374-68399393 None:intergenic 25.0%
! AAGATACATATGGTAATTTC+TGG - chr2.1:68400104-68400123 None:intergenic 25.0%
! CAAGCATAAAAAGATACATA+TGG - chr2.1:68400114-68400133 None:intergenic 25.0%
! CTTGATTATAATGAAACCAT+TGG + chr2.1:68400231-68400250 MS.gene002675:CDS 25.0%
! TAACCATCTAATTCTTTCTT+TGG - chr2.1:68399375-68399394 None:intergenic 25.0%
! TATGCAATCTTATACAAAAG+CGG - chr2.1:68398255-68398274 None:intergenic 25.0%
!! AAAATGTTGCAGAAATTTTG+AGG + chr2.1:68398718-68398737 MS.gene002675:intron 25.0%
!! AAGATTCAGTTTTTGTGAAT+TGG + chr2.1:68398051-68398070 MS.gene002675:intron 25.0%
!! AATTGGGTATTTTTCACATT+AGG + chr2.1:68398452-68398471 MS.gene002675:intron 25.0%
!! AGATTCAGTTTTTGTGAATT+GGG + chr2.1:68398052-68398071 MS.gene002675:intron 25.0%
!! ATTGGGTATTTTTCACATTA+GGG + chr2.1:68398453-68398472 MS.gene002675:intron 25.0%
!! CTTTTCAACTTTAGATCTTT+TGG + chr2.1:68398753-68398772 MS.gene002675:intron 25.0%
!!! AATTGTTCATTTGTTGTTCT+TGG + chr2.1:68397979-68397998 MS.gene002675:intron 25.0%
!!! GCTATTGAATTTGTTTTATG+AGG + chr2.1:68399034-68399053 MS.gene002675:intron 25.0%
!!! TCTTGGGTGTTTTTAATTTT+GGG + chr2.1:68398024-68398043 MS.gene002675:intron 25.0%
!!! TTCTTGGGTGTTTTTAATTT+TGG + chr2.1:68398023-68398042 MS.gene002675:intron 25.0%
AACAACTGCTCATCATTATT+GGG - chr2.1:68399492-68399511 None:intergenic 30.0%
AACTTCAGAGACTATATTAG+TGG - chr2.1:68399672-68399691 None:intergenic 30.0%
AATAAGCTCTTTATCCAAAC+AGG - chr2.1:68398187-68398206 None:intergenic 30.0%
ACCATCTAATTCTTTCTTTG+GGG - chr2.1:68399373-68399392 None:intergenic 30.0%
AGCATAAGATTGAACCAAAA+TGG + chr2.1:68398926-68398945 MS.gene002675:CDS 30.0%
CAAAAAGATCTAACTGATTG+AGG - chr2.1:68399762-68399781 None:intergenic 30.0%
CAATCTTATGCTTGAAACAA+TGG - chr2.1:68398918-68398937 None:intergenic 30.0%
CAGTTCATGAACAATTTGAA+TGG + chr2.1:68399418-68399437 MS.gene002675:intron 30.0%
GAATCTTCTGATTGAACATT+TGG + chr2.1:68400143-68400162 MS.gene002675:intron 30.0%
GAGGACTTATGAAAATAAGT+TGG + chr2.1:68398352-68398371 MS.gene002675:intron 30.0%
GCATAAGATTGAACCAAAAT+GGG + chr2.1:68398927-68398946 MS.gene002675:CDS 30.0%
GGGTGGAAAAAATTTCTATT+TGG + chr2.1:68399208-68399227 MS.gene002675:CDS 30.0%
GTTCTTCATCAATACCTTAT+TGG + chr2.1:68399636-68399655 MS.gene002675:intron 30.0%
TAACAACTGCTCATCATTAT+TGG - chr2.1:68399493-68399512 None:intergenic 30.0%
TACATGGAATATAAAGCCAA+TGG - chr2.1:68400250-68400269 None:intergenic 30.0%
TCTGATTGAACATTTGGTAA+TGG + chr2.1:68400149-68400168 MS.gene002675:intron 30.0%
TTGAATCAATAGGAACTAGA+TGG + chr2.1:68399281-68399300 MS.gene002675:intron 30.0%
! AACTCTTGTAATTTGTCAGA+TGG + chr2.1:68399983-68400002 MS.gene002675:intron 30.0%
! AGCTTCAATCTTTTCACTTT+AGG + chr2.1:68398159-68398178 MS.gene002675:intron 30.0%
! CTCAATCAGTTAGATCTTTT+TGG + chr2.1:68399760-68399779 MS.gene002675:intron 30.0%
! CTGATTGAACATTTGGTAAT+GGG + chr2.1:68400150-68400169 MS.gene002675:intron 30.0%
! GCTTCAATCTTTTCACTTTA+GGG + chr2.1:68398160-68398179 MS.gene002675:intron 30.0%
! TAGTCTGAAATTATCATGGA+GGG + chr2.1:68399793-68399812 MS.gene002675:intron 30.0%
! TGTTGATGTCTTCGATTTTA+GGG - chr2.1:68397714-68397733 None:intergenic 30.0%
! TTAGTCTGAAATTATCATGG+AGG + chr2.1:68399792-68399811 MS.gene002675:intron 30.0%
! TTCTTGGTAACTTTAGTTGT+TGG + chr2.1:68399119-68399138 MS.gene002675:intron 30.0%
! TTGAGGATTTTCAATATCAG+AGG - chr2.1:68399745-68399764 None:intergenic 30.0%
! TTGTTGATGTCTTCGATTTT+AGG - chr2.1:68397715-68397734 None:intergenic 30.0%
! TTTCTACTGTTGAAGAGTTT+TGG + chr2.1:68397908-68397927 MS.gene002675:CDS 30.0%
!! AATCTTTTGAGAGACTGTTT+AGG - chr2.1:68398421-68398440 None:intergenic 30.0%
!! GTTCCTACTTTTGAATCAAT+AGG + chr2.1:68399271-68399290 MS.gene002675:intron 30.0%
!! GTTCCTATTGATTCAAAAGT+AGG - chr2.1:68399277-68399296 None:intergenic 30.0%
!! TCGTGTTACTGAAATTTTCA+TGG + chr2.1:68398832-68398851 MS.gene002675:intron 30.0%
!! TCTCTGAAGTTTATAGACTT+TGG + chr2.1:68399681-68399700 MS.gene002675:intron 30.0%
!! TGATTGAACATTTGGTAATG+GGG + chr2.1:68400151-68400170 MS.gene002675:intron 30.0%
!! TTGAATTGTGCTATTTCTGT+AGG + chr2.1:68400334-68400353 MS.gene002675:intron 30.0%
!!! TTTTAAAATTGCTGGGAGTT+AGG - chr2.1:68399886-68399905 None:intergenic 30.0%
AAAGTCCAAGAATTCTCAAG+AGG - chr2.1:68397817-68397836 None:intergenic 35.0%
AAGTCCAAGAATTCTCAAGA+GGG - chr2.1:68397816-68397835 None:intergenic 35.0%
ACCCCAAAGAAAGAATTAGA+TGG + chr2.1:68399369-68399388 MS.gene002675:intron 35.0%
AGTTGTGAATGTCAGGAATA+GGG + chr2.1:68399188-68399207 MS.gene002675:CDS 35.0%
ATGAGCAGTTGTTATGTGAA+TGG + chr2.1:68399499-68399518 MS.gene002675:intron 35.0%
ATTGGAGAGCAATTTGATCA+TGG + chr2.1:68399147-68399166 MS.gene002675:CDS 35.0%
CTTTAGTTGTTGGCAATGAT+TGG + chr2.1:68399129-68399148 MS.gene002675:intron 35.0%
GATCATGGAGATGAAATTTG+TGG + chr2.1:68399162-68399181 MS.gene002675:CDS 35.0%
GATTTAACATGAAGTGACAC+AGG - chr2.1:68400299-68400318 None:intergenic 35.0%
TAGATCCAAAAGTCAAGCTT+GGG + chr2.1:68397858-68397877 MS.gene002675:CDS 35.0%
TGATACTAGCTGGTTGTATA+CGG + chr2.1:68399008-68399027 MS.gene002675:CDS 35.0%
TTCCACCATTAGCACAAATA+GGG - chr2.1:68398957-68398976 None:intergenic 35.0%
TTGAGAATTCTTGGACTTTC+TGG + chr2.1:68397818-68397837 MS.gene002675:CDS 35.0%
TTTCCACCATTAGCACAAAT+AGG - chr2.1:68398958-68398977 None:intergenic 35.0%
! AACAGTAGAAAGAGTGTAGA+CGG - chr2.1:68397899-68397918 None:intergenic 35.0%
! AGCTTCGATCTTTTCACTTT+AGG + chr2.1:68398543-68398562 MS.gene002675:intron 35.0%
! GCTTCGATCTTTTCACTTTA+GGG + chr2.1:68398544-68398563 MS.gene002675:intron 35.0%
! GTATCTTTTTATGCTTGTGC+TGG + chr2.1:68400117-68400136 MS.gene002675:intron 35.0%
! GTTATATTTCAGGTGAGCAT+CGG + chr2.1:68400189-68400208 MS.gene002675:intron 35.0%
! TCTTGTAATTTGTCAGATGG+AGG + chr2.1:68399986-68400005 MS.gene002675:intron 35.0%
! TTTGTGCTAATGGTGGAAAA+TGG + chr2.1:68398959-68398978 MS.gene002675:CDS 35.0%
!! ATGGACTGTGCAATTTTCTA+GGG + chr2.1:68398978-68398997 MS.gene002675:CDS 35.0%
!! CATGTGTGTGTTTTTGTTCT+TGG + chr2.1:68399103-68399122 MS.gene002675:intron 35.0%
!! CTTTTTCACTTTTGGTCTTC+TGG + chr2.1:68397946-68397965 MS.gene002675:intron 35.0%
!!! AATGGACTGTGCAATTTTCT+AGG + chr2.1:68398977-68398996 MS.gene002675:CDS 35.0%
AATTCTCAAGAGGGTGAGAT+TGG - chr2.1:68397807-68397826 None:intergenic 40.0%
ACACAGGCATAAAAACACAC+AGG - chr2.1:68400283-68400302 None:intergenic 40.0%
AGATCCAAAAGTCAAGCTTG+GGG + chr2.1:68397859-68397878 MS.gene002675:CDS 40.0%
CAGTTGTGAATGTCAGGAAT+AGG + chr2.1:68399187-68399206 MS.gene002675:CDS 40.0%
CTAGATCCAAAAGTCAAGCT+TGG + chr2.1:68397857-68397876 MS.gene002675:CDS 40.0%
GAAGACCCTATTTGTGCTAA+TGG + chr2.1:68398949-68398968 MS.gene002675:CDS 40.0%
GTGGTTCACTCTAACCAATA+AGG - chr2.1:68399653-68399672 None:intergenic 40.0%
TGTGATTTGATCGTCGTTGA+TGG - chr2.1:68397689-68397708 None:intergenic 40.0%
! ACAGTAGAAAGAGTGTAGAC+GGG - chr2.1:68397898-68397917 None:intergenic 40.0%
! CAAATAGGGTCTTCCCATTT+TGG - chr2.1:68398943-68398962 None:intergenic 40.0%
! CACAGGCAGTTTTACTTACA+TGG - chr2.1:68400266-68400285 None:intergenic 40.0%
! TGGACTGTGCAATTTTCTAG+GGG + chr2.1:68398979-68398998 MS.gene002675:CDS 40.0%
! TTTCACTTTAGGGACCTGTT+TGG + chr2.1:68398170-68398189 MS.gene002675:intron 40.0%
!! CTACTGTTGAAGAGTTTTGG+AGG + chr2.1:68397911-68397930 MS.gene002675:CDS 40.0%
!! GACTTTTGGATCTAGCTTGA+GGG - chr2.1:68397852-68397871 None:intergenic 40.0%
!! TGACTTTTGGATCTAGCTTG+AGG - chr2.1:68397853-68397872 None:intergenic 40.0%
!!! ATCGTCGTTGATGGATTTTG+GGG - chr2.1:68397680-68397699 None:intergenic 40.0%
!!! GATCGTCGTTGATGGATTTT+GGG - chr2.1:68397681-68397700 None:intergenic 40.0%
!!! TGATCGTCGTTGATGGATTT+TGG - chr2.1:68397682-68397701 None:intergenic 40.0%
ACTAGCTGGTTGTATACGGT+AGG + chr2.1:68399012-68399031 MS.gene002675:intron 45.0%
CATTCACCATCCTAGCAAGT+CGG + chr2.1:68398876-68398895 MS.gene002675:CDS 45.0%
CTCACCCTCTTGAGAATTCT+TGG + chr2.1:68397809-68397828 MS.gene002675:CDS 45.0%
GACCCTATTTGTGCTAATGG+TGG + chr2.1:68398952-68398971 MS.gene002675:CDS 45.0%
GGGGAAAATCTGATACTAGC+TGG + chr2.1:68398998-68399017 MS.gene002675:CDS 45.0%
TGTGAATGTCAGGAATAGGG+TGG + chr2.1:68399191-68399210 MS.gene002675:CDS 45.0%
! ACTACCCCAAGCTTGACTTT+TGG - chr2.1:68397866-68397885 None:intergenic 45.0%
GTGGAGCAGTTGTGAATGTC+AGG + chr2.1:68399181-68399200 MS.gene002675:CDS 50.0%
TGCTGCTAATGAAGCTGCTC+AGG + chr2.1:68399239-68399258 MS.gene002675:CDS 50.0%
! AAGAGGGTGAGATTGGTGAC+CGG - chr2.1:68397800-68397819 None:intergenic 50.0%
!! AGGTGAGCATCGGAAAACAG+TGG + chr2.1:68400199-68400218 MS.gene002675:intron 50.0%
!! GAGCATCGGAAAACAGTGGA+AGG + chr2.1:68400203-68400222 MS.gene002675:CDS 50.0%
ATTTCCAAACCCCTCACCGC+CGG + chr2.1:68397778-68397797 MS.gene002675:CDS 55.0%
CATCCTAGCAAGTCGGCTGT+TGG + chr2.1:68398883-68398902 MS.gene002675:CDS 55.0%
GGTGAGGGGTTTGGAAATGG+CGG - chr2.1:68397776-68397795 None:intergenic 55.0%
!! CAACAGCCGACTTGCTAGGA+TGG - chr2.1:68398885-68398904 None:intergenic 55.0%
GAGGGGTTTGGAAATGGCGG+AGG - chr2.1:68397773-68397792 None:intergenic 60.0%
GGCGGTGAGGGGTTTGGAAA+TGG - chr2.1:68397779-68397798 None:intergenic 60.0%
! AGATTGGTGACCGGCGGTGA+GGG - chr2.1:68397791-68397810 None:intergenic 60.0%
! AGGGTGAGATTGGTGACCGG+CGG - chr2.1:68397797-68397816 None:intergenic 60.0%
!! GCTCCAACAGCCGACTTGCT+AGG - chr2.1:68398889-68398908 None:intergenic 60.0%
! GAGATTGGTGACCGGCGGTG+AGG - chr2.1:68397792-68397811 None:intergenic 65.0%
! GATTGGTGACCGGCGGTGAG+GGG - chr2.1:68397790-68397809 None:intergenic 65.0%
GTGACCGGCGGTGAGGGGTT+TGG - chr2.1:68397785-68397804 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 68397661 68400406 68397661 ID=MS.gene002675
chr2.1 mRNA 68397661 68400406 68397661 ID=MS.gene002675.t1;Parent=MS.gene002675
chr2.1 exon 68397661 68397932 68397661 ID=MS.gene002675.t1.exon1;Parent=MS.gene002675.t1
chr2.1 CDS 68397661 68397932 68397661 ID=cds.MS.gene002675.t1;Parent=MS.gene002675.t1
chr2.1 exon 68398864 68399029 68398864 ID=MS.gene002675.t1.exon2;Parent=MS.gene002675.t1
chr2.1 CDS 68398864 68399029 68398864 ID=cds.MS.gene002675.t1;Parent=MS.gene002675.t1
chr2.1 exon 68399135 68399260 68399135 ID=MS.gene002675.t1.exon3;Parent=MS.gene002675.t1
chr2.1 CDS 68399135 68399260 68399135 ID=cds.MS.gene002675.t1;Parent=MS.gene002675.t1
chr2.1 exon 68400201 68400266 68400201 ID=MS.gene002675.t1.exon4;Parent=MS.gene002675.t1
chr2.1 CDS 68400201 68400266 68400201 ID=cds.MS.gene002675.t1;Parent=MS.gene002675.t1
chr2.1 exon 68400356 68400406 68400356 ID=MS.gene002675.t1.exon5;Parent=MS.gene002675.t1
chr2.1 CDS 68400356 68400406 68400356 ID=cds.MS.gene002675.t1;Parent=MS.gene002675.t1
Gene Sequence

>MS.gene002675

ATGGTTGTAGAAGAAACCCCAAAATCCATCAACGACGATCAAATCACAAACCCTAAAATCGAAGACATCAACAACAATCTCGAAGAAAGAGAGATCGACGACGATGATTCCTCCGCCATTTCCAAACCCCTCACCGCCGGTCACCAATCTCACCCTCTTGAGAATTCTTGGACTTTCTGGTTCGATAACCCTCAAGCTAGATCCAAAAGTCAAGCTTGGGGTAGTTCGATTCGACCCGTCTACACTCTTTCTACTGTTGAAGAGTTTTGGAGGTAAAAATTCTATCTTTTTCACTTTTGGTCTTCTGGCTCTTGAAAAAATTGTTCATTTGTTGTTCTTGGATGAAAAGATTCATTTTTTATTTCTTGGGTGTTTTTAATTTTGGGTTAAAAGATTCAGTTTTTGTGAATTGGGTATTTTTTACATTAGGGTTTGTGATTCATTTTTAGTTAATTGGGCATTTCTTGTTTGTAAAATGTTGAAGAATTTTTTAGGTAAAGCTTCAATCTTTTCACTTTAGGGACCTGTTTGGATAAAGAGCTTATTTGCAGCATATATTACAAGCGTTAATCAATATGCTTATCATGATATCCGCTTTTGTATAAGATTGCATAAGCTGTTTTATAGCAAAAAAATAATAACGCTGAATTGTTTTTTTATATGCTATAAGTTATTTCTATAAGCTATATTTGAGGACTTATGAAAATAAGTTGGAAAAAGCTTATGAAAATTGTCATAAGCTGTTTTCATAAATTCTCCTAAACAGTCTCTCAAAAGATTCAGTTTTTATGAATTGGGTATTTTTCACATTAGGGTTTGTGATTCATTTTTAGTTAATTGGGTATTTCTTGTTTGTACAATGTTGTAGAATTTTTTAGGTAAAGCTTCGATCTTTTCACTTTAGGGACCTGTTTGGATAAAGAGCTTATTTGCAGCATATATCTCAAGCGTTTACTATGTGCTTATCATGATAAGCACTAATGTATAAGCTTGCATAAAAATATCGTTCATTTATTGTTTCTTGATTATTTTTGTATCTTGGGTATTTCTTGTTTGTAAAATGTTGCAGAAATTTTGAGGTAAAAATTCAATCTTTTCAACTTTAGATCTTTTGGGTCATCAAAAATATTGTTCATTTATTGATTCTTGGATATTTTTGTTTCTTAGTTATTCGTGTTACTGAAATTTTCATGGTTTTTTCAGCATTTACAATAACATTCACCATCCTAGCAAGTCGGCTGTTGGAGCTGATTTCCATTGTTTCAAGCATAAGATTGAACCAAAATGGGAAGACCCTATTTGTGCTAATGGTGGAAAATGGACTGTGCAATTTTCTAGGGGAAAATCTGATACTAGCTGGTTGTATACGGTAGGCTATTGAATTTGTTTTATGAGGGTATTATTTTGATTATTCTTTGTGATGAATTAAAGTACTAATTGGTCATGTGTGTGTTTTTGTTCTTGGTAACTTTAGTTGTTGGCAATGATTGGAGAGCAATTTGATCATGGAGATGAAATTTGTGGAGCAGTTGTGAATGTCAGGAATAGGGTGGAAAAAATTTCTATTTGGACTAAGAATGCTGCTAATGAAGCTGCTCAGGTAATTTCTTGTTCCTACTTTTGAATCAATAGGAACTAGATGGTTATGATGTTGAAGAGTAATATTATGATTTTCAAACTAAAGATGTGCATTTGTTATGTGACTGAGACCCCAAAGAAAGAATTAGATGGTTATTATAATGGCATGTATTCAATCACAGTTCATGAACAATTTGAATGGACATTTTTTACATTTTTGTTGCTTCAATGCATGTGTTTGTTGTTTTCTCCCAATAATGATGAGCAGTTGTTATGTGAATGGCTCACTCTTATCATTGCTGTTAAATATGAATGTAAAAAGCATGTATTCACAGTTCATAAGCTATTTAAATAGACAGTTTTCACGTTTTTTGTTTATTCAATGTCTCTGTTTCTTGTTCTTCATCAATACCTTATTGGTTAGAGTGAACCACTAATATAGTCTCTGAAGTTTATAGACTTTGGTCAATTTAGCATCTGTAATTTACAAAATTTCTTAATGACCTCTGATATTGAAAATCCTCAATCAGTTAGATCTTTTTGGTCGATCAATTTAGTCTGAAATTATCATGGAGGGACTAATGTGATTGAGAATTTAAATTTCAGGGATCACTTGTGAATTTTATTGATTTTAAGACAAATTGCCTAACTCCCAGCAATTTTAAAACTTAATTTATGATTCACTATTATACATATGCTGCGACAATTCCATATAAGCTATTTTTTGATAGAAGAGAATTTAAAAACTCTTGTAATTTGTCAGATGGAGGATGAATGTGTGATTATTATACATATGCTGCGACAATTCCATATAATTTACTCTTGAACTTGCAAAGCTAACTTTCTTTCATGACTTTTTAGACCAGAAATTACCATATGTATCTTTTTATGCTTGTGCTGGTGTGAATCTTCTGATTGAACATTTGGTAATGGGGAACATATAAACTTCTGTTATATTTCAGGTGAGCATCGGAAAACAGTGGAAGGAGTTTCTTGATTATAATGAAACCATTGGCTTTATATTCCATGTAAGTAAAACTGCCTGTGTGTTTTTATGCCTGTGTCACTTCATGTTAAATCACACTAACTTCTGTTTTGAATTGTGCTATTTCTGTAGGATGATGCAAAGAAGCTTGACAGAGCTGCTAAAAACAAATATGTTGTATGA
Protein sequence

>MS.gene002675.t1

MVVEETPKSINDDQITNPKIEDINNNLEEREIDDDDSSAISKPLTAGHQSHPLENSWTFWFDNPQARSKSQAWGSSIRPVYTLSTVEEFWSIYNNIHHPSKSAVGADFHCFKHKIEPKWEDPICANGGKWTVQFSRGKSDTSWLYTLLAMIGEQFDHGDEICGAVVNVRNRVEKISIWTKNAANEAAQVSIGKQWKEFLDYNETIGFIFHDDAKKLDRAAKNKYVV