AlfalfaGEDB | MS.gene73500

M. sativa cultivar XinJiangDaYe / MS.gene73500

KEGG: K11996
GO: GO:0002097 GO:0002098 GO:0002143 GO:0003674 GO:0003824 GO:0004792 GO:0005575 GO:0005829 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008641 GO:0009451 GO:0009987 GO:0016070 GO:0016740 GO:0016782 GO:0016783 GO:0016874 GO:0016877 GO:0034227 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0110165 GO:1901360
PFAM: PF00581.23 PF00899.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73500.t1	XP_003617620.1	95.9	464	11	1	4	459	2	465	1.70E-248	868.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73500.t1	Q9ZNW0	68.6	465	138	3	1	459	2	464	1.3e-180	634.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73500.t1	G7K8M6	95.9	464	11	1	4	459	2	465	1.2e-248	868.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene002676	MS.gene73500	PPI
MS.gene004357	MS.gene73500	PPI
MS.gene006731	MS.gene73500	PPI
MS.gene021573	MS.gene73500	PPI
MS.gene05791	MS.gene73500	PPI
MS.gene05792	MS.gene73500	PPI
MS.gene070760	MS.gene73500	PPI
MS.gene070761	MS.gene73500	PPI
MS.gene29165	MS.gene73500	PPI
MS.gene43604	MS.gene73500	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73500.t1	MTR_5g093580	95.905	464	11	1	4	459	2	465	0.0	909
MS.gene73500.t1	MTR_8g037355	28.105	153	108	2	85	236	9	160	6.81e-20	92.8
MS.gene73500.t1	MTR_4g131690	28.796	191	119	5	68	242	28	217	5.83e-15	77.0
MS.gene73500.t1	MTR_5g072480	28.824	170	106	5	53	209	559	726	5.09e-12	68.9
MS.gene73500.t1	MTR_5g072480	28.824	170	106	5	53	209	558	725	5.13e-12	68.9
MS.gene73500.t1	MTR_5g072480	28.824	170	106	5	53	209	375	542	5.32e-12	68.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73500.t1	AT5G55130	68.602	465	138	3	1	459	2	464	0.0	667
MS.gene73500.t1	AT5G55130	63.871	465	133	4	1	459	2	437	0.0	606
MS.gene73500.t1	AT5G55130	71.709	357	99	1	103	459	6	360	0.0	550
MS.gene73500.t1	AT2G21470	28.049	164	116	2	77	238	5	168	1.93e-19	91.7
MS.gene73500.t1	AT2G21470	28.049	164	116	2	77	238	5	168	2.24e-19	91.3
MS.gene73500.t1	AT2G21470	28.049	164	116	2	77	238	5	168	2.46e-19	91.3
MS.gene73500.t1	AT5G19180	29.586	169	105	3	87	242	49	216	4.92e-15	77.4
MS.gene73500.t1	AT5G19180	29.586	169	105	3	87	242	49	216	5.81e-15	77.0
MS.gene73500.t1	AT5G06460	31.613	155	95	4	64	209	471	623	1.11e-14	77.4
MS.gene73500.t1	AT2G30110	28.824	170	109	5	49	209	460	626	1.27e-11	67.8

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Find 107 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATGAAGAAGAGGAAAA+AGG	0.342101	5.2:-78675376	MS.gene73500:CDS
AACTACATAGCTAATAAGTG+GGG	0.673584	5.2:+78675197	None:intergenic
AAGAAATCATACCACATGCA+AGG	0.576332	5.2:+78676086	None:intergenic
AATGGAATGTTCAAGGAATT+TGG	0.178410	5.2:+78675445	None:intergenic
ACAATCTTGAAATGGTGAGC+TGG	0.563760	5.2:+78675475	None:intergenic
ACAGCGGCACCTGATACGAG+GGG	0.670870	5.2:+78676856	None:intergenic
ACCATACCCTACAAATGGAT+TGG	0.482774	5.2:-78679722	MS.gene73500:CDS
ACTATCTACAATCACAACGG+TGG	0.719133	5.2:-78676724	MS.gene73500:CDS
ACTTCTCATAATCAAATTCT+CGG	0.443462	5.2:+78676121	None:intergenic
ACTTCTGCTAAGGATATTGT+TGG	0.395111	5.2:-78675259	MS.gene73500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGATGAAGAAGAGGAAAA+AGG	-	chr5.2:78679702-78679721	MS.gene73500:CDS	30.0%
AACTTTAATCACTGATGAGT+TGG	+	chr5.2:78679126-78679145	None:intergenic	30.0%
AATGGAATGTTCAAGGAATT+TGG	+	chr5.2:78679636-78679655	None:intergenic	30.0%
AGACTATTTGGAAGAGATTA+TGG	+	chr5.2:78676548-78676567	None:intergenic	30.0%
AGCAATGTAGTTATAAATGG+AGG	+	chr5.2:78676123-78676142	None:intergenic	30.0%
ATCTCATCTACAATTCATGT+CGG	-	chr5.2:78677177-78677196	MS.gene73500:intron	30.0%
ATGTAGACAGCATGATTTAT+AGG	-	chr5.2:78678138-78678157	MS.gene73500:intron	30.0%
ATTCAGCGGTAATATTCAAA+AGG	+	chr5.2:78677426-78677445	None:intergenic	30.0%
CACAGCAATGTAGTTATAAA+TGG	+	chr5.2:78676126-78676145	None:intergenic	30.0%
CAGTAAAGATCAAAGAAAAC+AGG	+	chr5.2:78677393-78677412	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	78675207	78679893	78675207	ID=MS.gene73500
chr5.2	mRNA	78675207	78679893	78675207	ID=MS.gene73500.t1;Parent=MS.gene73500
chr5.2	exon	78679662	78679893	78679662	ID=MS.gene73500.t1.exon1;Parent=MS.gene73500.t1
chr5.2	CDS	78679662	78679893	78679662	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78678343	78678435	78678343	ID=MS.gene73500.t1.exon2;Parent=MS.gene73500.t1
chr5.2	CDS	78678343	78678435	78678343	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78678208	78678263	78678208	ID=MS.gene73500.t1.exon3;Parent=MS.gene73500.t1
chr5.2	CDS	78678208	78678263	78678208	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78677941	78678005	78677941	ID=MS.gene73500.t1.exon4;Parent=MS.gene73500.t1
chr5.2	CDS	78677941	78678005	78677941	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78677758	78677832	78677758	ID=MS.gene73500.t1.exon5;Parent=MS.gene73500.t1
chr5.2	CDS	78677758	78677832	78677758	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78677539	78677617	78677539	ID=MS.gene73500.t1.exon6;Parent=MS.gene73500.t1
chr5.2	CDS	78677539	78677617	78677539	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78676840	78676878	78676840	ID=MS.gene73500.t1.exon7;Parent=MS.gene73500.t1
chr5.2	CDS	78676840	78676878	78676840	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78676641	78676749	78676641	ID=MS.gene73500.t1.exon8;Parent=MS.gene73500.t1
chr5.2	CDS	78676641	78676749	78676641	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78676426	78676544	78676426	ID=MS.gene73500.t1.exon9;Parent=MS.gene73500.t1
chr5.2	CDS	78676426	78676544	78676426	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78676098	78676214	78676098	ID=MS.gene73500.t1.exon10;Parent=MS.gene73500.t1
chr5.2	CDS	78676098	78676214	78676098	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1
chr5.2	exon	78675207	78675602	78675207	ID=MS.gene73500.t1.exon11;Parent=MS.gene73500.t1
chr5.2	CDS	78675207	78675602	78675207	ID=cds.MS.gene73500.t1;Parent=MS.gene73500.t1

Gene Sequence

>MS.gene73500

ATGTCTTCTTCTTCTTCGGCTTCAGAGATTCTTCAAGAACTTGAATCATTGAAGAACGAAAAAACCAAAATCGAACAAAAAATATCATCTTTAGAATCTCAATTGAAAGATATTAATCTCCCAAAACACGCTTCCAATGGTTCTTCTTCACCATACCCTACAAATGGATTGGAACCTCACATGATTCATAGATACAGTCGCCACCTTGTTCTTCCTTCCTTTGGTGTTCAAGGTACTACACATTCATTCATCTAAATTACACTATCAAATATTCAAGAACAATTTTTTTATTTTTTTATTTTATTGAAAATGTTAGATATAGTTGGATATTAATTAGAATTAGAATTAGTTTGGGGGTTGCTTATTTCAACTGGTTTTGAGCTAAGGGTTAACTAAGTTGAAGGGTTAGGGTTCAAATGATCAAACCTGACATAAATTTTGAGAGAACTTATGAAAACAACTTATAGCTTATACAATTTAACTTTGCTATAAAAATAGCTTATATATAAGCACTTTTCATGATAGGCACTTATGCTATAAGATGCAAATAAGTTATGTTTTCAAACAGGGCCTTAGGCTGTATCATAAGTGCTCATCATATAAGTATTTATCTATAAGCTGTTTCTATAGAAAAACTTAATTAAAATTGAACTCTTTTCATATTAGTTGTAAGTTGTTTTCATAACCAGAGTAACATAATTCGTGTACTTTGGTGGAGAACCGCGATCCAATTCAAGAGTTCCGAATTCGGGTACCTCAGCGAATTTGGTACGCGTGAATTCATGGAGAATTCGCACAAATTGCGGTACCAAGCGTTCCAGTGAAAGGATAATGCTGATAAGTTTGTGCTATAAAATTGAGAATTAAATAAATAAACAGTGGTCAAATGCACCGTAAGCTTAGGATAAGGACACCTCCATTTATAACTACATTGCTGTGTTATCCTCAAATCCACATGATTTGCAATGCATTTGGTTGCTTTTATATATAGCTTTCTTCTCTTCTTCTCTCAAATCTTTTGATGTGGGACTTAAAGAACAACGTTGTGCACCTACGCTACTAGCTTTGATGGGGTTATTATCCTTTTTTGCATGATGTGGCTCATTCTATAATATAGTGTTGGGCTTATTTCTTTTTAGCATTAATATTGCGAACCAACGCGTACCGAAGCTCTATATCCCCATACGTACCGAATTCTTCATGTGTAGTGTACTGCGAACCCGTACCCGTACCGCGTACCAACGCGAATTGCGAATTATGTGACTATGTTCATAACATATTCTTGAGACCTTATAGAAATAAGTTAAAAAAAACTTACAAACATGTTATAAGCAATTTCCATAATCTCTTCCAAATAGTCTCACTAGTGTTTATGCTAGGAGATAATTTCAAATAAACAACCTTTAGAATATAGTAATCGGTTTTGAATTATATTTTGTTTATTTCCATGGTTTGCAGGGCAGGCAAATCTGCTGAAGTCGTCAATTTTAGTTGTGGGAGCCGGAGGATTGGGTGCTCCTGCTTTGTTATATTTTGCAGCTTCTGGTGTTGGTAAAATATGATACTTTTCTAATGATTGTTGCCTTGTTCTATAATCTTGTTAAAGCTTACATTTTAAAAAATTGCACAGGTAAATTGGGTATTGTTGATCATGACAAGGTTGAGCTCAGTAATATGCATAGACAGGTATAGTTTTATTTGCTAAACTTGTGGTATGGCTAGTGCAACTTGTACGATGTAGCTTGTTTATTGTTCATGTGTGTTGTGTGGCCCTTTTTGCACTTCTGCGAGTACAAGTTGTAGTTAGCATGTATCTTTTTGTTTACTTTTTATTTCCTGTTTGTAACTTTGATCTTATTCACTTCAACTCTTTCATGCCATGTTTCAGATTATACACACCGAAGCATATATTGGTCAGCCAAAAGTGAAATCCGCTGCTGCTGCTTGTCGCGCGTATGTTGCTCTCTTTTATCTCATCTACAATTCATGTCGGTTTTGGATCAAATGTTCGTAACAAATTTGTATCATTATTGTTTGATCTAAGTTTTTGTGAATATTTAGGGTCAACTCATCTGTTGAAGTCGTGGAACACGAAGAAGCTCTGCGAACTTCCAATGCTTTGGAAATTTTCAGCAAGTATGTTTCCTATCTTCAATATGATCGGTCTTTCTTTTTTCTTAAGGCCTGTTTTCTTTGATCTTTACTGTTTTTGTTTCCCTTTTGAATATTACCGCTGAATCCGTGTGATTTCTTTTCTTATCATGTGAATTTGCTAGATACGATATAATAGTGGATGCAACAGATAATGCTCCCACCCGGTATCTGATCAGTGACTGCTGTGTGGTACTAGGAAAGGTAAATCTTACTATTTCAATATAAGAACAAATTGCGACTGTCTGAATTGATGTCAAACAAGGCTTTTCCTTTGTTACTATATTATTTTAGGGTCATGAATGAATACACTCTGTAAGTCATTTGATTCGTTGATGCACATGATATCTCGATTAATTTAGAAAAAGGGAAGTTATGTGTGTGTTGAAACATAAAATATTGAAACTTTCACATATATAATGACACAGATGAAGGTTGATTTCAACAAATAATATTTAGATTTCTGAGCTAGGTTGGACGCCTTCTATTGATTGTGGGCGAAATGCCTTTATTAATTTCAATAGTATTCAAAATGTGTGCTATATGAGTTATTGCCCCTAAAATCCTTGTGCAATTTTCCCACTAAGCGGCAAATCAAGATTTTGAATTCATTGTAAATTTGCAATTTTTTAGCTCTCTCATACACCAGAATTAAATATGAATAAAAAGATCCAAAAAATGAAATTTTGGACCAAATACATTTGACTTTTTGAATATTTTTTTGTTATTTTCACTCCATTAACGTTTCCCCAACCATATTTTTAGCTTATTTTGTCTCATATGTAGACAGCATGATTTATAGGAAAAGTTAAGTTACTTCTATTGCAACTTCTTTAATTAATTGCTTTCGTTGTACACATGCAGCCCCTCGTATCAGGTGCCGCTGTGGGATTGGAAGGACAGGTATATGTCTAATTATTTTGTCGCATCCCTTTATGTCTTTGATTATATCACATTATTTTGAGGTACACTCATGGAAAACATATGATGCAGCTCACTATCTACAATCACAACGGTGGTCCTTGCTATCGATGCCTCTTTCCTACTCCACCACCTAGATCAGCATGCCAAAGTTGCGCCGACAATGGAGTACTAGGAGTAGGTATGTTGTTTGGTTCTTGTACATCTCATTCGGACCATTTTTCCCCTGCTGTTGCGATTTGTTCTGAATTTCTGATATTTAGTCTGTTCATTTTAGTTCCTGGTATAATTGGCTGTCTCCAAGCTCTCGAGGCAATTAAGATTGCTGCTGCAGTTGGTGAACCACTCTCCGGAAGGATGCTTCTCTTTGATGCATTGTCTGCACGAATCCGGGTTGTATGTTAATAACTTCACAACCTTGCTTATGATCATTATTTTACGCATTGGCCAACGAGATGGAATTAAGATGTAGAATTCTAATGATAGAAATGGATGCTTATTTGTGCAGGATGCATTATGAGAAAAAAATTAAATTAAGATTGTCTATATGAAGTTCTTTCTTCTTATATAATATATTAGCTAAGGATTATATAATCTTATTGCACAGGTGAAAATCAGAGGAAGGTCCATGCATTGTGAAGCTTGTGGAGAAAATGCAGCATTTAATAAGCAGTACTTCCGAGAATTTGATTATGAGAAGTTCACTCAGACTCCCTTGCATGTGGTATGATTTCTTCTAACTTAAAAACAATTATACACTTTACTAATTGCCGGACACAAATATTATAAGTTAGTTTTTGTATTTAGGAAAGTTAAAATCTGCCATCCAACACAGGGTGCCAATCCAACTCATCAGTGATTAAAGTTTTATTTTGGTTGTTATACTTGTTTTCTATTGCCCAAGTGTATTATTATCTAACCTTTGCTAATGGAATTTAATCCCTGTACTGATCCCAAAACTTACTAATTTTGAAAAGTAGTAATAATATTCGAGAAAAGGAGCCATGCACTATCGCATAAATTAGTTTTACACTGTGGTCTAATGAAAAAATTCAATATTTTGTAGTTATTTTTAAAATACACTTACAAAATGGGCTTATGTGACAATATGATGAGTTTTTTCATTGGATGTCATTGTTAACCTAGTTTTTGCTATACTGCATCTTCATTAAACTAATAATATTTTTCATATCACTAATGCATTCCAATGCAAATGCAGCCTCCGTTGAAGTTAAATCTACTTCCTAGTGAATCAAGGATCAGCAGCATGGAGTACAAAGAAATAACTCTAAACAAGGAACCACATGTGCTTGTTGATGTTAGACCAGCTCACCATTTCAAGATTGTGTCACTTCCAAATTCCTTGAACATTCCATTCTCAGCTTTAGAGTCTAGGTTGCCGGAAATATCATCAATTTTGAAGAAAGATGAAGAAGAGGAAAAAGGCGCGGTTTCTGAATCAAGTGCACAATTATATGTAGTATGCAGAAGAGGAAATGATTCTCAAAGAGCTGTTCAATACCTTCACAAAATGGGTTTTACTTCTGCTAAGGATATTGTTGGGGGTTTGGAGTCTTGGGCTCTAAATGTTGATCCTAACTTCCCCACTTATTAG

Protein sequence

>MS.gene73500.t1

MSSSSSASEILQELESLKNEKTKIEQKISSLESQLKDINLPKHASNGSSSPYPTNGLEPHMIHRYSRHLVLPSFGVQGQANLLKSSILVVGAGGLGAPALLYFAASGVGKLGIVDHDKVELSNMHRQIIHTEAYIGQPKVKSAAAACRAVNSSVEVVEHEEALRTSNALEIFSKYDIIVDATDNAPTRYLISDCCVVLGKPLVSGAAVGLEGQLTIYNHNGGPCYRCLFPTPPPRSACQSCADNGVLGVVPGIIGCLQALEAIKIAAAVGEPLSGRMLLFDALSARIRVVKIRGRSMHCEACGENAAFNKQYFREFDYEKFTQTPLHVPPLKLNLLPSESRISSMEYKEITLNKEPHVLVDVRPAHHFKIVSLPNSLNIPFSALESRLPEISSILKKDEEEEKGAVSESSAQLYVVCRRGNDSQRAVQYLHKMGFTSAKDIVGGLESWALNVDPNFPTY