AlfalfaGEDB | MS.gene003218

M. sativa cultivar XinJiangDaYe / MS.gene003218

KEGG: K05765
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005856 GO:0007015 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0015629 GO:0016043 GO:0022411 GO:0030042 GO:0032984 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0051261 GO:0071840 GO:0097435 GO:0110165
PFAM: PF00241.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003218.t1	XP_003594440.1	98.6	139	2	0	1	139	1	139	4.50E-72	280.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003218.t1	Q9FVI1	85.5	138	20	0	1	138	1	138	6.0e-65	248.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003218.t1	G7IFU0	98.6	139	2	0	1	139	1	139	3.2e-72	280.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene003218	MS.gene063026	PPI
MS.gene003218	MS.gene023659	PPI
MS.gene003218	MS.gene057694	PPI
MS.gene003218	MS.gene020366	PPI
MS.gene003218	MS.gene23488	PPI
MS.gene003218	MS.gene037986	PPI
MS.gene003218	MS.gene34500	PPI
MS.gene003218	MS.gene97005	PPI
MS.gene003218	MS.gene037980	PPI
MS.gene018706	MS.gene003218	PPI
MS.gene023659	MS.gene003218	PPI
MS.gene003218	MS.gene47853	PPI
MS.gene003218	MS.gene27898	PPI
MS.gene037983	MS.gene003218	PPI
MS.gene003218	MS.gene037982	PPI
MS.gene003218	MS.gene02797	PPI
MS.gene003218	MS.gene037990	PPI
MS.gene003218	MS.gene24057	PPI
MS.gene003218	MS.gene41437	PPI
MS.gene003218	MS.gene058991	PPI
MS.gene87667	MS.gene003218	PPI
MS.gene003218	MS.gene003535	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003218.t1	MTR_2g028670	98.561	139	2	0	1	139	1	139	2.99e-102	288
MS.gene003218.t1	MTR_8g088210	87.770	139	17	0	1	139	1	139	3.19e-93	266
MS.gene003218.t1	MTR_8g088210	87.121	132	17	0	8	139	1	132	1.66e-87	251
MS.gene003218.t1	MTR_1g068950	79.856	139	28	0	1	139	1	139	1.64e-84	243
MS.gene003218.t1	MTR_8g098470	71.739	138	37	1	1	138	1	136	5.18e-76	222
MS.gene003218.t1	MTR_5g010430	69.343	137	40	1	2	138	4	138	9.89e-71	209
MS.gene003218.t1	MTR_1g101190	70.073	137	39	1	2	138	463	597	5.92e-66	210
MS.gene003218.t1	MTR_7g096890	59.124	137	56	0	3	139	10	146	6.18e-64	192
MS.gene003218.t1	MTR_4g073590	55.556	135	60	0	4	138	8	142	1.65e-56	173
MS.gene003218.t1	MTR_1g076170	55.797	138	61	0	2	139	10	147	1.38e-51	160
MS.gene003218.t1	MTR_4g073590	54.023	87	40	0	4	90	8	94	4.34e-33	112

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003218.t1	AT5G59880	80.576	139	27	0	1	139	1	139	1.33e-86	249
MS.gene003218.t1	AT3G46010	80.576	139	27	0	1	139	1	139	2.31e-86	248
MS.gene003218.t1	AT3G46010	80.435	138	27	0	2	139	13	150	1.15e-85	247
MS.gene003218.t1	AT5G59890	80.576	139	27	0	1	139	1	139	3.15e-84	243
MS.gene003218.t1	AT3G46000	79.137	139	27	1	1	139	1	137	6.51e-81	234
MS.gene003218.t1	AT3G46000	78.417	139	28	1	1	139	20	156	5.22e-80	233
MS.gene003218.t1	AT5G59890	79.545	132	27	0	8	139	1	132	1.23e-78	228
MS.gene003218.t1	AT5G59890	79.545	132	27	0	8	139	1	132	1.23e-78	228
MS.gene003218.t1	AT3G46000	78.030	132	27	1	8	139	1	130	2.28e-75	220
MS.gene003218.t1	AT4G00680	69.065	139	43	0	1	139	1	139	1.73e-74	218
MS.gene003218.t1	AT1G01750	69.784	139	42	0	1	139	1	139	2.87e-74	218
MS.gene003218.t1	AT4G00680	68.841	138	43	0	2	139	12	149	2.26e-73	216
MS.gene003218.t1	AT1G01750	69.565	138	42	0	2	139	7	144	2.72e-73	216
MS.gene003218.t1	AT4G25590	68.841	138	41	1	1	138	1	136	1.33e-72	213
MS.gene003218.t1	AT5G52360	69.565	138	40	1	1	138	1	136	2.28e-72	213
MS.gene003218.t1	AT5G59880	71.223	139	25	1	1	139	1	124	2.61e-72	212
MS.gene003218.t1	AT4G25590	67.176	131	41	1	8	138	1	129	5.26e-67	199
MS.gene003218.t1	AT2G31200	54.015	137	63	0	3	139	10	146	3.15e-57	175
MS.gene003218.t1	AT4G34970	53.623	138	64	0	1	138	3	140	5.77e-56	171
MS.gene003218.t1	AT2G16700	51.111	135	66	0	4	138	8	142	9.30e-53	163
MS.gene003218.t1	AT2G16700	51.145	131	64	0	8	138	1	131	7.86e-51	158
MS.gene003218.t1	AT2G16700	51.145	131	64	0	8	138	1	131	7.86e-51	158
MS.gene003218.t1	AT3G45990	56.522	138	49	3	8	139	1	133	3.22e-48	152
MS.gene003218.t1	AT2G16700	50.575	87	43	0	4	90	8	94	3.40e-30	105

Find 27 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGACTGCAAGTTAAAGTTTA+TGG	0.155260	2.1:-62305760	MS.gene003218:CDS
CTCCCTCTTGAACCTTTCTT+TGG	0.182021	2.1:+62305393	None:intergenic
CTTCTCACTCTTGCTGTATC+AGG	0.259069	2.1:+62305436	None:intergenic
GGCAACTGATCCTACTGAAA+TGG	0.363842	2.1:-62305344	MS.gene003218:CDS
ATTCAGAATCATTATTTAGT+TGG	0.389131	2.1:+62305297	None:intergenic
TGGAATTCAAATAGAGTTGC+AGG	0.390254	2.1:-62305365	MS.gene003218:CDS
TTATGGAGCTCAAGGCAAAA+AGG	0.429370	2.1:-62305743	MS.gene003218:CDS
GCAACTGATCCTACTGAAAT+GGG	0.470343	2.1:-62305343	MS.gene003218:CDS
CTACAGGCAAATGCAGCATC+TGG	0.471777	2.1:-62305798	MS.gene003218:intron
ACTTTAACTTGCAGTCATCA+TGG	0.482180	2.1:+62305766	None:intergenic
GTTAAAGTTTATGGAGCTCA+AGG	0.502426	2.1:-62305751	MS.gene003218:CDS
CGATCTTATACACTATGAAC+CGG	0.509573	2.1:+62305715	None:intergenic
TTTATGCAAGCACCAAAGAA+AGG	0.510363	2.1:-62305405	MS.gene003218:CDS
GCATAACGGCACTCATCAGC+AGG	0.530155	2.1:+62305615	None:intergenic
GGCAAATGCAGCATCTGGTA+TGG	0.530905	2.1:-62305793	MS.gene003218:CDS
CAAGTCATTGTGGAAAAGCT+TGG	0.539988	2.1:-62305678	MS.gene003218:CDS
AGAAAGGTTCAAGAGGGAGC+TGG	0.559858	2.1:-62305389	MS.gene003218:CDS
GAAACAGAAGCAAGTCATTG+TGG	0.565595	2.1:-62305688	MS.gene003218:CDS
GAATCATTATTTAGTTGGCA+CGG	0.570158	2.1:+62305302	None:intergenic
GCACCAAAGAAAGGTTCAAG+AGG	0.572284	2.1:-62305396	MS.gene003218:CDS
AGGTTCAAGAGGGAGCTGGA+TGG	0.608161	2.1:-62305385	MS.gene003218:CDS
AAGCTTGGTGAGCCAGCTGA+AGG	0.610538	2.1:-62305663	MS.gene003218:CDS
CAAAATCATAGACAGCATAA+CGG	0.612434	2.1:+62305601	None:intergenic
ACATCAAGACCCATTTCAGT+AGG	0.621902	2.1:+62305334	None:intergenic
CACCAAAGAAAGGTTCAAGA+GGG	0.636143	2.1:-62305395	MS.gene003218:CDS
AACGGCACTCATCAGCAGGA+AGG	0.636804	2.1:+62305619	None:intergenic
GTTCATAGTGTATAAGATCG+AGG	0.648241	2.1:-62305712	MS.gene003218:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAGCAGAATTTTTTTCAT+TGG	-	chr2.1:62305768-62305787	MS.gene003218:CDS	20.0%
!!!	AAAGCAGAATTTTTTTCATT+GGG	-	chr2.1:62305769-62305788	MS.gene003218:CDS	20.0%
!!!	AATGAAAAAAATTCTGCTTT+TGG	+	chr2.1:62305769-62305788	None:intergenic	20.0%
!!!	ATGAAAAAAATTCTGCTTTT+GGG	+	chr2.1:62305768-62305787	None:intergenic	20.0%
!!!	TGAAAAAAATTCTGCTTTTG+GGG	+	chr2.1:62305767-62305786	None:intergenic	25.0%
	CAAAATCATAGACAGCATAA+CGG	+	chr2.1:62305722-62305741	None:intergenic	30.0%
	TGACTGCAAGTTAAAGTTTA+TGG	-	chr2.1:62305560-62305579	MS.gene003218:CDS	30.0%
!!	AATGTTTGCTTTTCGATACA+TGG	-	chr2.1:62305796-62305815	MS.gene003218:intron	30.0%
	AGATAGGAAGTAAACAACCA+AGG	+	chr2.1:62305832-62305851	None:intergenic	35.0%
	CCAACAGAATTAATGCAGAT+AGG	+	chr2.1:62305848-62305867	None:intergenic	35.0%
	CCTATCTGCATTAATTCTGT+TGG	-	chr2.1:62305845-62305864	MS.gene003218:intron	35.0%
	CGATCTTATACACTATGAAC+CGG	+	chr2.1:62305608-62305627	None:intergenic	35.0%
	GTTAAAGTTTATGGAGCTCA+AGG	-	chr2.1:62305569-62305588	MS.gene003218:CDS	35.0%
	GTTCATAGTGTATAAGATCG+AGG	-	chr2.1:62305608-62305627	MS.gene003218:CDS	35.0%
	GTTTCTAACTGCAATTCTAC+AGG	-	chr2.1:62305506-62305525	MS.gene003218:intron	35.0%
	TGGAATTCAAATAGAGTTGC+AGG	-	chr2.1:62305955-62305974	MS.gene003218:intron	35.0%
	TTTATGCAAGCACCAAAGAA+AGG	-	chr2.1:62305915-62305934	MS.gene003218:intron	35.0%
!	AAATTTTCATAGCCTTCAGC+TGG	+	chr2.1:62305672-62305691	None:intergenic	35.0%
!	TACATGGCTATAGTTTTCCT+TGG	-	chr2.1:62305812-62305831	MS.gene003218:intron	35.0%
!!	ACTTTAACTTGCAGTCATCA+TGG	+	chr2.1:62305557-62305576	None:intergenic	35.0%
!!!	GCAGAATTTTTTTCATTGGG+TGG	-	chr2.1:62305772-62305791	MS.gene003218:CDS	35.0%
	ACATCAAGACCCATTTCAGT+AGG	+	chr2.1:62305989-62306008	None:intergenic	40.0%
	CAAGTCATTGTGGAAAAGCT+TGG	-	chr2.1:62305642-62305661	MS.gene003218:CDS	40.0%
	CACCAAAGAAAGGTTCAAGA+GGG	-	chr2.1:62305925-62305944	MS.gene003218:intron	40.0%
	GAAACAGAAGCAAGTCATTG+TGG	-	chr2.1:62305632-62305651	MS.gene003218:CDS	40.0%
	GCAACTGATCCTACTGAAAT+GGG	-	chr2.1:62305977-62305996	MS.gene003218:intron	40.0%
	TTATGGAGCTCAAGGCAAAA+AGG	-	chr2.1:62305577-62305596	MS.gene003218:CDS	40.0%
!!	ATTCTGTTGGCTTTGTGTGT+AGG	-	chr2.1:62305858-62305877	MS.gene003218:intron	40.0%
	CTCCCTCTTGAACCTTTCTT+TGG	+	chr2.1:62305930-62305949	None:intergenic	45.0%
	GCACCAAAGAAAGGTTCAAG+AGG	-	chr2.1:62305924-62305943	MS.gene003218:intron	45.0%
	GGCAACTGATCCTACTGAAA+TGG	-	chr2.1:62305976-62305995	MS.gene003218:intron	45.0%
	TCAAGGCAAAAAGGACACAC+CGG	-	chr2.1:62305586-62305605	MS.gene003218:CDS	45.0%
	TCATGTGATTGCTGATCTGC+TGG	-	chr2.1:62305454-62305473	MS.gene003218:intron	45.0%
!	CTTCTCACTCTTGCTGTATC+AGG	+	chr2.1:62305887-62305906	None:intergenic	45.0%
	AGAAAGGTTCAAGAGGGAGC+TGG	-	chr2.1:62305931-62305950	MS.gene003218:intron	50.0%
	CTACAGGCAAATGCAGCATC+TGG	-	chr2.1:62305522-62305541	MS.gene003218:intron	50.0%
	GGCAAATGCAGCATCTGGTA+TGG	-	chr2.1:62305527-62305546	MS.gene003218:intron	50.0%
	AACGGCACTCATCAGCAGGA+AGG	+	chr2.1:62305704-62305723	None:intergenic	55.0%
	AGGTTCAAGAGGGAGCTGGA+TGG	-	chr2.1:62305935-62305954	MS.gene003218:intron	55.0%
	GCATAACGGCACTCATCAGC+AGG	+	chr2.1:62305708-62305727	None:intergenic	55.0%
!	AAGCTTGGTGAGCCAGCTGA+AGG	-	chr2.1:62305657-62305676	MS.gene003218:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	62305312	62306030	62305312	ID=MS.gene003218
chr2.1	mRNA	62305312	62306030	62305312	ID=MS.gene003218.t1;Parent=MS.gene003218
chr2.1	exon	62306028	62306030	62306028	ID=MS.gene003218.t1.exon1;Parent=MS.gene003218.t1
chr2.1	CDS	62306028	62306030	62306028	ID=cds.MS.gene003218.t1;Parent=MS.gene003218.t1
chr2.1	exon	62305549	62305814	62305549	ID=MS.gene003218.t1.exon2;Parent=MS.gene003218.t1
chr2.1	CDS	62305549	62305814	62305549	ID=cds.MS.gene003218.t1;Parent=MS.gene003218.t1
chr2.1	exon	62305312	62305462	62305312	ID=MS.gene003218.t1.exon3;Parent=MS.gene003218.t1
chr2.1	CDS	62305312	62305462	62305312	ID=cds.MS.gene003218.t1;Parent=MS.gene003218.t1

Gene Sequence

>MS.gene003218

ATGGTTAGCAACTAAAAACTTCTCATCTGATTCAGACTTTTAGTCTTTCTTTCAGATCATGTCATGAACTGTCTTTTTCTTTGTTTTTCTCTTTTGTTGAGTAACAAGTTAAATGACGAAGTTTACTTATTTGTGTACAATCTCATGTGATTGCTGATCTGCTGGTGCATGACATTGATGATTGATTATGATGTGTTTCTAACTGCAATTCTACAGGCAAATGCAGCATCTGGTATGGCAGTCCATGATGACTGCAAGTTAAAGTTTATGGAGCTCAAGGCAAAAAGGACACACCGGTTCATAGTGTATAAGATCGAGGAGAAACAGAAGCAAGTCATTGTGGAAAAGCTTGGTGAGCCAGCTGAAGGCTATGAAAATTTCACTGCATGCCTTCCTGCTGATGAGTGCCGTTATGCTGTCTATGATTTTGAGTATCTGACAGAAGAGAATGTCCCCAAAAGCAGAATTTTTTTCATTGGGTGGTAATGTTTGCTTTTCGATACATGGCTATAGTTTTCCTTGGTTGTTTACTTCCTATCTGCATTAATTCTGTTGGCTTTGTGTGTAGGTCGCCTGATACAGCAAGAGTGAGAAGCAAGATGATTTATGCAAGCACCAAAGAAAGGTTCAAGAGGGAGCTGGATGGAATTCAAATAGAGTTGCAGGCAACTGATCCTACTGAAATGGGTCTTGATGTGTTCAAAAGCCGTGCCAACTAA

Protein sequence

>MS.gene003218.t1

MANAASGMAVHDDCKLKFMELKAKRTHRFIVYKIEEKQKQVIVEKLGEPAEGYENFTACLPADECRYAVYDFEYLTEENVPKSRIFFIGWSPDTARVRSKMIYASTKERFKRELDGIQIELQATDPTEMGLDVFKSRAN