AlfalfaGEDB | MS.gene037986

M. sativa cultivar XinJiangDaYe / MS.gene037986

KEGG: K05765
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005856 GO:0007015 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0015629 GO:0016043 GO:0022411 GO:0030042 GO:0032984 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0051261 GO:0071840 GO:0097435 GO:0110165
PFAM: PF00241.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037986.t1	XP_013468343.1	97.8	138	3	0	34	171	2	139	2.30E-70	275

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037986.t1	Q9ZSK3	81.9	138	25	0	34	171	2	139	1.2e-62	240.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037986.t1	G7ZZK8	97.8	138	3	0	34	171	2	139	1.7e-70	275.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene063026	MS.gene037986	PPI
MS.gene87667	MS.gene037986	PPI
MS.gene037986	MS.gene037982	PPI
MS.gene24057	MS.gene037986	PPI
MS.gene037986	MS.gene27898	PPI
MS.gene037990	MS.gene037986	PPI
MS.gene057694	MS.gene037986	PPI
MS.gene41437	MS.gene037986	PPI
MS.gene003218	MS.gene037986	PPI
MS.gene018706	MS.gene037986	PPI
MS.gene023659	MS.gene037986	PPI
MS.gene037986	MS.gene058991	PPI
MS.gene037983	MS.gene037986	PPI
MS.gene037986	MS.gene97005	PPI
MS.gene037986	MS.gene020366	PPI
MS.gene037982	MS.gene037986	PPI
MS.gene058991	MS.gene037986	PPI
MS.gene037986	MS.gene23488	PPI
MS.gene037986	MS.gene34500	PPI
MS.gene003535	MS.gene037986	PPI
MS.gene47853	MS.gene037986	PPI
MS.gene02797	MS.gene037986	PPI
MS.gene037980	MS.gene037986	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037986.t1	MTR_1g068950	97.826	138	3	0	34	171	2	139	1.61e-97	278
MS.gene037986.t1	MTR_2g028670	78.261	138	30	0	34	171	2	139	3.20e-81	236
MS.gene037986.t1	MTR_8g088210	76.087	138	33	0	34	171	2	139	7.10e-78	228
MS.gene037986.t1	MTR_5g010430	74.286	140	34	1	31	170	1	138	9.13e-77	225
MS.gene037986.t1	MTR_8g098470	71.533	137	37	1	34	170	2	136	2.54e-73	216
MS.gene037986.t1	MTR_8g088210	75.000	132	33	0	40	171	1	132	5.48e-73	216
MS.gene037986.t1	MTR_1g101190	72.464	138	36	1	32	169	461	596	1.30e-67	216
MS.gene037986.t1	MTR_7g096890	59.574	141	57	0	31	171	6	146	3.98e-65	196
MS.gene037986.t1	MTR_1g076170	61.594	138	53	0	34	171	10	147	4.70e-59	181
MS.gene037986.t1	MTR_4g073590	60.000	135	54	0	36	170	8	142	6.03e-58	178
MS.gene037986.t1	MTR_4g073590	60.920	87	34	0	36	122	8	94	2.19e-35	119

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037986.t1	AT5G59890	81.884	138	25	0	34	171	2	139	2.26e-84	244
MS.gene037986.t1	AT3G46010	81.884	138	25	0	34	171	13	150	8.03e-84	244
MS.gene037986.t1	AT3G46010	81.884	138	25	0	34	171	2	139	1.66e-83	243
MS.gene037986.t1	AT5G59890	81.061	132	25	0	40	171	1	132	1.48e-79	232
MS.gene037986.t1	AT5G59890	81.061	132	25	0	40	171	1	132	1.48e-79	232
MS.gene037986.t1	AT1G01750	72.917	144	39	0	28	171	1	144	7.88e-79	231
MS.gene037986.t1	AT4G00680	72.917	144	38	1	28	171	7	149	1.58e-78	230
MS.gene037986.t1	AT5G59880	74.638	138	35	0	34	171	2	139	9.57e-78	228
MS.gene037986.t1	AT4G00680	73.913	138	36	0	34	171	2	139	1.33e-77	228
MS.gene037986.t1	AT1G01750	74.638	138	35	0	34	171	2	139	4.02e-77	226
MS.gene037986.t1	AT3G46000	76.812	138	30	1	34	171	2	137	2.25e-76	224
MS.gene037986.t1	AT3G46000	76.812	138	30	1	34	171	21	156	4.76e-76	224
MS.gene037986.t1	AT4G25590	75.182	137	32	1	34	170	2	136	6.56e-74	218
MS.gene037986.t1	AT5G52360	75.182	137	32	1	34	170	2	136	7.37e-72	213
MS.gene037986.t1	AT3G46000	75.758	132	30	1	40	171	1	130	1.66e-71	212
MS.gene037986.t1	AT4G25590	74.046	131	32	1	40	170	1	129	5.57e-69	205
MS.gene037986.t1	AT5G59880	63.768	138	35	1	34	171	2	124	1.26e-62	189
MS.gene037986.t1	AT2G31200	58.156	141	59	0	31	171	6	146	1.43e-61	187
MS.gene037986.t1	AT2G16700	53.676	136	63	0	36	171	8	143	5.52e-54	168
MS.gene037986.t1	AT4G34970	53.285	137	64	0	35	171	5	141	5.78e-54	168
MS.gene037986.t1	AT2G16700	53.788	132	61	0	40	171	1	132	8.44e-52	162
MS.gene037986.t1	AT2G16700	53.788	132	61	0	40	171	1	132	8.44e-52	162
MS.gene037986.t1	AT3G45990	51.449	138	56	3	40	171	1	133	3.09e-43	140
MS.gene037986.t1	AT2G16700	49.020	102	52	0	36	137	8	109	1.58e-32	112

Find 30 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTACCCTTGAAATATC+AGG	0.195342	1.4:-81890714	None:intergenic
GTATATCATTTACAAGATTA+AGG	0.294112	1.4:+81890412	MS.gene037986:CDS
TACACTAATCTAATGGTAAT+GGG	0.308098	1.4:+81890221	None:intergenic
CTACACTAATCTAATGGTAA+TGG	0.419474	1.4:+81890220	None:intergenic
TTTATGCTAGCTCCAAAGAT+AGG	0.430442	1.4:+81890745	MS.gene037986:CDS
AAATCATCGTAACCATTTGC+AGG	0.435601	1.4:-81890473	None:intergenic
GAAGGTGCCCTGATATTTCA+AGG	0.462729	1.4:+81890706	MS.gene037986:intron
TTACAGGCGAATGCAGCATC+AGG	0.463408	1.4:+81890326	MS.gene037986:CDS
GCTCCAAAGATAGGTTCAAA+AGG	0.469123	1.4:+81890754	MS.gene037986:CDS
GCATATCGACACTCGTCGGC+AGG	0.482779	1.4:-81890509	None:intergenic
TGTGGCGTTGAAAGTGTGTA+TGG	0.483413	1.4:+81890262	MS.gene037986:CDS
GGTTCAAAAGGGAGCTTGAT+GGG	0.491075	1.4:+81890766	MS.gene037986:CDS
ACATCAGATGCATCAACAAA+AGG	0.502015	1.4:-81890293	None:intergenic
GGCGAATGCAGCATCAGGTA+TGG	0.502868	1.4:+81890331	MS.gene037986:CDS
GCCCTGATATTTCAAGGGTA+AGG	0.520257	1.4:+81890712	MS.gene037986:CDS
CAAGTTGTTGTTGACAAGGT+TGG	0.532386	1.4:+81890446	MS.gene037986:CDS
CTCCAAAGATAGGTTCAAAA+GGG	0.546134	1.4:+81890755	MS.gene037986:CDS
AGCTACTGATCCTACTGAGA+TGG	0.549628	1.4:+81890806	MS.gene037986:CDS
AGGTTCAAAAGGGAGCTTGA+TGG	0.551429	1.4:+81890765	MS.gene037986:CDS
GTTGTTAGTATGCTTGTTTG+TGG	0.553370	1.4:+81890244	MS.gene037986:CDS
TCCTTACCCTTGAAATATCA+GGG	0.558692	1.4:-81890713	None:intergenic
AAGGTTGGTGATCCTGCAAA+TGG	0.580318	1.4:+81890461	MS.gene037986:CDS
GAAGCAAGTTGTTGTTGACA+AGG	0.580658	1.4:+81890442	MS.gene037986:CDS
AAGGTGCCCTGATATTTCAA+GGG	0.592018	1.4:+81890707	MS.gene037986:intron
CTTGTAAATGATATACCGAT+AGG	0.592223	1.4:-81890405	None:intergenic
ACATCAAGATCCATCTCAGT+AGG	0.597944	1.4:-81890816	None:intergenic
AACAGCATATCGACACTCGT+CGG	0.602599	1.4:-81890513	None:intergenic
TCAAGGCGAAGAGGACCTAT+CGG	0.606898	1.4:+81890390	MS.gene037986:CDS
ACACTAATCTAATGGTAATG+GGG	0.642233	1.4:+81890222	None:intergenic
TCACAAAAGATTAATTGACA+CGG	0.694452	1.4:-81890848	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAATCAATCATAAAAAA+TGG	-	chr1.4:81890656-81890675	None:intergenic	10.0%
!!	GTATATCATTTACAAGATTA+AGG	+	chr1.4:81890412-81890431	MS.gene037986:CDS	20.0%
!	AAGCAACAAAATGATATACT+CGG	-	chr1.4:81890680-81890699	None:intergenic	25.0%
!	CAACAGTTAACTTATAGATT+TGG	+	chr1.4:81890625-81890644	MS.gene037986:intron	25.0%
!!	TGAATGCAAGTTAAAGTTTT+TGG	+	chr1.4:81890364-81890383	MS.gene037986:CDS	25.0%
!!	TGATGTTTGTGAAATTTTAC+AGG	+	chr1.4:81890310-81890329	MS.gene037986:CDS	25.0%
!!!	ATTTTGTTGCTTTTGTTTGA+AGG	+	chr1.4:81890688-81890707	MS.gene037986:intron	25.0%
	AGCAACAAAATGATATACTC+GGG	-	chr1.4:81890679-81890698	None:intergenic	30.0%
	CTTGTAAATGATATACCGAT+AGG	-	chr1.4:81890408-81890427	None:intergenic	30.0%
!!	TGAAGAAAATTCTGCTTTTC+TGG	-	chr1.4:81890571-81890590	None:intergenic	30.0%
!!!	TGATTTTGATTTTGTCACTG+AGG	+	chr1.4:81890538-81890557	MS.gene037986:CDS	30.0%
	AAATCATCGTAACCATTTGC+AGG	-	chr1.4:81890476-81890495	None:intergenic	35.0%
	ACATCAGATGCATCAACAAA+AGG	-	chr1.4:81890296-81890315	None:intergenic	35.0%
	CTCCAAAGATAGGTTCAAAA+GGG	+	chr1.4:81890755-81890774	MS.gene037986:CDS	35.0%
	GTTGTTAGTATGCTTGTTTG+TGG	+	chr1.4:81890244-81890263	MS.gene037986:CDS	35.0%
	TCCTTACCCTTGAAATATCA+GGG	-	chr1.4:81890716-81890735	None:intergenic	35.0%
	TTTATGCTAGCTCCAAAGAT+AGG	+	chr1.4:81890745-81890764	MS.gene037986:CDS	35.0%
!	GCAGAATTTTCTTCATAGCT+TGG	+	chr1.4:81890576-81890595	MS.gene037986:CDS	35.0%
!!!	GCTTGGTAATGTCAACTTTT+TGG	+	chr1.4:81890593-81890612	MS.gene037986:intron	35.0%
!!!	GTTAAAGTTTTTGGAGCTCA+AGG	+	chr1.4:81890373-81890392	MS.gene037986:CDS	35.0%
	ACATCAAGATCCATCTCAGT+AGG	-	chr1.4:81890819-81890838	None:intergenic	40.0%
	CTCCTTACCCTTGAAATATC+AGG	-	chr1.4:81890717-81890736	None:intergenic	40.0%
	GCTCCAAAGATAGGTTCAAA+AGG	+	chr1.4:81890754-81890773	MS.gene037986:CDS	40.0%
!	AAGGTGCCCTGATATTTCAA+GGG	+	chr1.4:81890707-81890726	MS.gene037986:intron	40.0%
!	CAAGTTGTTGTTGACAAGGT+TGG	+	chr1.4:81890446-81890465	MS.gene037986:CDS	40.0%
!	CTCCCTTTTGAACCTATCTT+TGG	-	chr1.4:81890760-81890779	None:intergenic	40.0%
!	GAAGCAAGTTGTTGTTGACA+AGG	+	chr1.4:81890442-81890461	MS.gene037986:CDS	40.0%
	AACAGCATATCGACACTCGT+CGG	-	chr1.4:81890516-81890535	None:intergenic	45.0%
	AGCTACTGATCCTACTGAGA+TGG	+	chr1.4:81890806-81890825	MS.gene037986:CDS	45.0%
	AGGTTCAAAAGGGAGCTTGA+TGG	+	chr1.4:81890765-81890784	MS.gene037986:CDS	45.0%
	GCCCTGATATTTCAAGGGTA+AGG	+	chr1.4:81890712-81890731	MS.gene037986:CDS	45.0%
	GGTTCAAAAGGGAGCTTGAT+GGG	+	chr1.4:81890766-81890785	MS.gene037986:CDS	45.0%
!	TGTGGCGTTGAAAGTGTGTA+TGG	+	chr1.4:81890262-81890281	MS.gene037986:CDS	45.0%
!!	AAGGTTGGTGATCCTGCAAA+TGG	+	chr1.4:81890461-81890480	MS.gene037986:CDS	45.0%
!!	GAAGGTGCCCTGATATTTCA+AGG	+	chr1.4:81890706-81890725	MS.gene037986:intron	45.0%
	TCAAGGCGAAGAGGACCTAT+CGG	+	chr1.4:81890390-81890409	MS.gene037986:CDS	50.0%
	TTACAGGCGAATGCAGCATC+AGG	+	chr1.4:81890326-81890345	MS.gene037986:CDS	50.0%
!!!	TTTTGGAGCTCAAGGCGAAG+AGG	+	chr1.4:81890381-81890400	MS.gene037986:CDS	50.0%
	GGCGAATGCAGCATCAGGTA+TGG	+	chr1.4:81890331-81890350	MS.gene037986:CDS	55.0%
	GCATATCGACACTCGTCGGC+AGG	-	chr1.4:81890512-81890531	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	81890233	81890860	81890233	ID=MS.gene037986
chr1.4	mRNA	81890233	81890860	81890233	ID=MS.gene037986.t1;Parent=MS.gene037986
chr1.4	exon	81890233	81890597	81890233	ID=MS.gene037986.t1.exon1;Parent=MS.gene037986.t1
chr1.4	CDS	81890233	81890597	81890233	ID=cds.MS.gene037986.t1;Parent=MS.gene037986.t1
chr1.4	exon	81890710	81890860	81890710	ID=MS.gene037986.t1.exon2;Parent=MS.gene037986.t1
chr1.4	CDS	81890710	81890860	81890710	ID=cds.MS.gene037986.t1;Parent=MS.gene037986.t1

Gene Sequence

>MS.gene037986

ATGGTAATGGGGTTGTTAGTATGCTTGTTTGTGGCGTTGAAAGTGTGTATGGAGAAATTTCCTTTTGTTGATGCATCTGATGTTTGTGAAATTTTACAGGCGAATGCAGCATCAGGTATGGATGTGAATGATGAATGCAAGTTAAAGTTTTTGGAGCTCAAGGCGAAGAGGACCTATCGGTATATCATTTACAAGATTAAGGTGAAGCAGAAGCAAGTTGTTGTTGACAAGGTTGGTGATCCTGCAAATGGTTACGATGATTTCACTGCTAATCTTCCTGCCGACGAGTGTCGATATGCTGTTTATGATTTTGATTTTGTCACTGAGGAGAATTGCCAGAAAAGCAGAATTTTCTTCATAGCTTGGTAATGTCAACTTTTTGGATCTTAAAGCAACAGTTAACTTATAGATTTGGATATTCCATTTTTTATGATTGATTTTAACCCGAGTATATCATTTTGTTGCTTTTGTTTGAAGGTGCCCTGATATTTCAAGGGTAAGGAGCAAGATGATTTATGCTAGCTCCAAAGATAGGTTCAAAAGGGAGCTTGATGGGATTCAAGTTGAGTTGCAAGCTACTGATCCTACTGAGATGGATCTTGATGTGTTCAAAAGCCGTGTCAATTAA

Protein sequence

>MS.gene037986.t1

MVMGLLVCLFVALKVCMEKFPFVDASDVCEILQANAASGMDVNDECKLKFLELKAKRTYRYIIYKIKVKQKQVVVDKVGDPANGYDDFTANLPADECRYAVYDFDFVTEENCQKSRIFFIAWCPDISRVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFKSRVN