AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037982


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037982.t1 MTR_1g068950 97.794 136 3 0 34 169 2 137 5.80e-96 274
MS.gene037982.t1 MTR_2g028670 78.676 136 29 0 34 169 2 137 4.24e-80 234
MS.gene037982.t1 MTR_8g088210 77.206 136 31 0 34 169 2 137 1.49e-77 227
MS.gene037982.t1 MTR_5g010430 74.820 139 33 1 31 169 1 137 1.44e-76 225
MS.gene037982.t1 MTR_8g098470 72.059 136 36 1 34 169 2 135 2.43e-73 216
MS.gene037982.t1 MTR_8g088210 76.154 130 31 0 40 169 1 130 1.27e-72 214
MS.gene037982.t1 MTR_1g101190 72.464 138 36 1 32 169 461 596 1.28e-67 216
MS.gene037982.t1 MTR_7g096890 60.432 139 55 0 31 169 6 144 7.43e-65 195
MS.gene037982.t1 MTR_1g076170 61.765 136 52 0 34 169 10 145 5.17e-58 178
MS.gene037982.t1 MTR_4g073590 60.448 134 53 0 36 169 8 141 7.12e-58 177
MS.gene037982.t1 MTR_4g073590 60.920 87 34 0 36 122 8 94 1.94e-35 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037982.t1 AT3G46010 82.353 136 24 0 34 169 13 148 8.37e-83 241
MS.gene037982.t1 AT5G59890 81.618 136 25 0 34 169 2 137 9.48e-83 240
MS.gene037982.t1 AT3G46010 82.353 136 24 0 34 169 2 137 1.75e-82 240
MS.gene037982.t1 AT5G59890 80.769 130 25 0 40 169 1 130 7.33e-78 228
MS.gene037982.t1 AT5G59890 80.769 130 25 0 40 169 1 130 7.33e-78 228
MS.gene037982.t1 AT4G00680 73.239 142 37 1 28 169 7 147 2.09e-77 227
MS.gene037982.t1 AT1G01750 72.535 142 39 0 28 169 1 142 2.48e-77 227
MS.gene037982.t1 AT5G59880 75.000 136 34 0 34 169 2 137 1.14e-76 225
MS.gene037982.t1 AT4G00680 74.265 136 35 0 34 169 2 137 1.71e-76 224
MS.gene037982.t1 AT1G01750 74.265 136 35 0 34 169 2 137 1.58e-75 222
MS.gene037982.t1 AT3G46000 77.206 136 29 1 34 169 2 135 3.67e-75 221
MS.gene037982.t1 AT3G46000 77.206 136 29 1 34 169 21 154 5.65e-75 221
MS.gene037982.t1 AT4G25590 75.735 136 31 1 34 169 2 135 6.27e-74 218
MS.gene037982.t1 AT5G52360 75.735 136 31 1 34 169 2 135 8.40e-72 213
MS.gene037982.t1 AT3G46000 76.154 130 29 1 40 169 1 128 2.32e-70 209
MS.gene037982.t1 AT4G25590 74.615 130 31 1 40 169 1 128 5.22e-69 205
MS.gene037982.t1 AT5G59880 63.971 136 34 1 34 169 2 122 1.36e-61 186
MS.gene037982.t1 AT2G31200 58.273 139 58 0 31 169 6 144 1.49e-60 184
MS.gene037982.t1 AT2G16700 54.478 134 61 0 36 169 8 141 5.97e-54 168
MS.gene037982.t1 AT4G34970 54.074 135 62 0 35 169 5 139 8.12e-54 167
MS.gene037982.t1 AT2G16700 54.615 130 59 0 40 169 1 130 9.44e-52 162
MS.gene037982.t1 AT2G16700 54.615 130 59 0 40 169 1 130 9.44e-52 162
MS.gene037982.t1 AT3G45990 51.471 136 55 3 40 169 1 131 8.37e-42 137
MS.gene037982.t1 AT2G16700 49.020 102 52 0 36 137 8 109 1.66e-32 112

Find 0 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATCAATCATAAAAAA+TGG - 50851:20790-20809 MS.gene037982:intergenic 10.0%
!! GTATATCATTTACAAGATTA+AGG + 50851:20546-20565 MS.gene037982:CDS 20.0%
! AAGCAACAAAATGATATACT+CGG - 50851:20814-20833 MS.gene037982:intergenic 25.0%
! CAACAGTTAACTTATAGATT+TGG + 50851:20759-20778 MS.gene037982:intron 25.0%
!! TGAATGCAAGTTAAAGTTTT+TGG + 50851:20498-20517 MS.gene037982:CDS 25.0%
!! TGATGTTTGTGAAATTTTAC+AGG + 50851:20444-20463 MS.gene037982:CDS 25.0%
!!! ATTTTGTTGCTTTTGTTTGA+AGG + 50851:20822-20841 MS.gene037982:intron 25.0%
AGCAACAAAATGATATACTC+GGG - 50851:20813-20832 MS.gene037981:intergenic 30.0%
CTTGTAAATGATATACCGAT+AGG - 50851:20542-20561 MS.gene037982:intergenic 30.0%
!! TGAAGAAAATTCTGCTTTTC+TGG - 50851:20705-20724 MS.gene037982:intergenic 30.0%
!!! TGATTTTGATTTTGTCACTG+AGG + 50851:20672-20691 MS.gene037982:CDS 30.0%
AAATCATCGTAACCATTTGC+AGG - 50851:20610-20629 MS.gene037981:intergenic 35.0%
ACATCAGATGCATCAACAAA+AGG - 50851:20430-20449 MS.gene037981:intergenic 35.0%
CTCCAAAGATAGGTTCAAAA+GGG + 50851:20889-20908 MS.gene037982:CDS 35.0%
GTTGTTAGTATGCTTGTTTG+TGG + 50851:20378-20397 MS.gene037982:CDS 35.0%
TCCTTACCCTTGAAATATCA+GGG - 50851:20850-20869 MS.gene037982:intergenic 35.0%
TTTATGCTAGCTCCAAAGAT+AGG + 50851:20879-20898 MS.gene037982:CDS 35.0%
! GCAGAATTTTCTTCATAGCT+TGG + 50851:20710-20729 MS.gene037982:CDS 35.0%
!!! GCTTGGTAATGTCAACTTTT+TGG + 50851:20727-20746 MS.gene037982:intron 35.0%
!!! GTTAAAGTTTTTGGAGCTCA+AGG + 50851:20507-20526 MS.gene037982:CDS 35.0%
ACATCAAGATCCATCTCAGT+AGG - 50851:20953-20972 MS.gene037981:intergenic 40.0%
CTCCTTACCCTTGAAATATC+AGG - 50851:20851-20870 MS.gene037982:intergenic 40.0%
GCTCCAAAGATAGGTTCAAA+AGG + 50851:20888-20907 MS.gene037982:CDS 40.0%
! AAGGTGCCCTGATATTTCAA+GGG + 50851:20841-20860 MS.gene037982:intron 40.0%
! CAAGTTGTTGTTGACAAGGT+TGG + 50851:20580-20599 MS.gene037982:CDS 40.0%
! CTCCCTTTTGAACCTATCTT+TGG - 50851:20894-20913 MS.gene037982:intergenic 40.0%
! GAAGCAAGTTGTTGTTGACA+AGG + 50851:20576-20595 MS.gene037982:CDS 40.0%
AACAGCATATCGACACTCGT+CGG - 50851:20650-20669 MS.gene037981:intergenic 45.0%
AGCTACTGATCCTACTGAGA+TGG + 50851:20940-20959 MS.gene037982:CDS 45.0%
AGGTTCAAAAGGGAGCTTGA+TGG + 50851:20899-20918 MS.gene037982:CDS 45.0%
GCCCTGATATTTCAAGGGTA+AGG + 50851:20846-20865 MS.gene037982:CDS 45.0%
GGTTCAAAAGGGAGCTTGAT+GGG + 50851:20900-20919 MS.gene037982:CDS 45.0%
! TGTGGCGTTGAAAGTGTGTA+TGG + 50851:20396-20415 MS.gene037982:CDS 45.0%
!! AAGGTTGGTGATCCTGCAAA+TGG + 50851:20595-20614 MS.gene037982:CDS 45.0%
!! GAAGGTGCCCTGATATTTCA+AGG + 50851:20840-20859 MS.gene037982:intron 45.0%
TCAAGGCGAAGAGGACCTAT+CGG + 50851:20524-20543 MS.gene037982:CDS 50.0%
TTACAGGCGAATGCAGCATC+AGG + 50851:20460-20479 MS.gene037982:CDS 50.0%
!!! TTTTGGAGCTCAAGGCGAAG+AGG + 50851:20515-20534 MS.gene037982:CDS 50.0%
GGCGAATGCAGCATCAGGTA+TGG + 50851:20465-20484 MS.gene037982:CDS 55.0%
GCATATCGACACTCGTCGGC+AGG - 50851:20646-20665 MS.gene037982:intergenic 60.0%


Chromosome Type Strat End Strand Name
50851 gene 20367 20985 20367 ID=MS.gene037982
50851 mRNA 20367 20985 20367 ID=MS.gene037982.t1;Parent=MS.gene037982
50851 exon 20367 20731 20367 ID=MS.gene037982.t1.exon1;Parent=MS.gene037982.t1
50851 CDS 20367 20731 20367 ID=cds.MS.gene037982.t1;Parent=MS.gene037982.t1
50851 exon 20844 20985 20844 ID=MS.gene037982.t1.exon2;Parent=MS.gene037982.t1
50851 CDS 20844 20985 20844 ID=cds.MS.gene037982.t1;Parent=MS.gene037982.t1
Gene Sequence

>MS.gene037982

ATGGTAATGGGGTTGTTAGTATGCTTGTTTGTGGCGTTGAAAGTGTGTATGGAGAAATTTCCTTTTGTTGATGCATCTGATGTTTGTGAAATTTTACAGGCGAATGCAGCATCAGGTATGGATGTGAATGATGAATGCAAGTTAAAGTTTTTGGAGCTCAAGGCGAAGAGGACCTATCGGTATATCATTTACAAGATTAAGGTGAAGCAGAAGCAAGTTGTTGTTGACAAGGTTGGTGATCCTGCAAATGGTTACGATGATTTCACTGCTAATCTTCCTGCCGACGAGTGTCGATATGCTGTTTATGATTTTGATTTTGTCACTGAGGAGAATTGCCAGAAAAGCAGAATTTTCTTCATAGCTTGGTAATGTCAACTTTTTGGATCTTAAAGCAACAGTTAACTTATAGATTTGGATATTCCATTTTTTATGATTGATTTTAACCCGAGTATATCATTTTGTTGCTTTTGTTTGAAGGTGCCCTGATATTTCAAGGGTAAGGAGCAAGATGATTTATGCTAGCTCCAAAGATAGGTTCAAAAGGGAGCTTGATGGGATTCAAGTTGAGTTGCAAGCTACTGATCCTACTGAGATGGATCTTGATGTGTTCAAAAGCCGT

Protein sequence

>MS.gene037982.t1

MVMGLLVCLFVALKVCMEKFPFVDASDVCEILQANAASGMDVNDECKLKFLELKAKRTYRYIIYKIKVKQKQVVVDKVGDPANGYDDFTANLPADECRYAVYDFDFVTEENCQKSRIFFIAWCPDISRVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFKSR