Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018706.t1 | XP_013449894.1 | 98.6 | 146 | 2 | 0 | 1 | 146 | 1 | 146 | 2.10E-75 | 291.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018706.t1 | Q8SAG3 | 87.0 | 146 | 16 | 1 | 1 | 146 | 1 | 143 | 2.7e-68 | 259.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018706.t1 | I3SXB9 | 98.6 | 146 | 2 | 0 | 1 | 146 | 1 | 146 | 1.5e-75 | 291.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene018706 | MS.gene063026 | PPI |
| MS.gene018706 | MS.gene023659 | PPI |
| MS.gene018706 | MS.gene058991 | PPI |
| MS.gene018706 | MS.gene003535 | PPI |
| MS.gene018706 | MS.gene037986 | PPI |
| MS.gene018706 | MS.gene057694 | PPI |
| MS.gene018706 | MS.gene34500 | PPI |
| MS.gene018706 | MS.gene003218 | PPI |
| MS.gene018706 | MS.gene47853 | PPI |
| MS.gene018706 | MS.gene87667 | PPI |
| MS.gene018706 | MS.gene27898 | PPI |
| MS.gene018706 | MS.gene037980 | PPI |
| MS.gene018706 | MS.gene037982 | PPI |
| MS.gene018706 | MS.gene02797 | PPI |
| MS.gene018706 | MS.gene037990 | PPI |
| MS.gene018706 | MS.gene24057 | PPI |
| MS.gene018706 | MS.gene41437 | PPI |
| MS.gene018706 | MS.gene037983 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018706.t1 | MTR_7g096890 | 98.630 | 146 | 2 | 0 | 1 | 146 | 1 | 146 | 9.05e-108 | 303 |
| MS.gene018706.t1 | MTR_1g076170 | 80.272 | 147 | 28 | 1 | 1 | 146 | 1 | 147 | 2.42e-79 | 231 |
| MS.gene018706.t1 | MTR_4g073590 | 68.148 | 135 | 43 | 0 | 11 | 145 | 8 | 142 | 2.26e-70 | 208 |
| MS.gene018706.t1 | MTR_1g068950 | 61.314 | 137 | 53 | 0 | 10 | 146 | 3 | 139 | 1.06e-66 | 199 |
| MS.gene018706.t1 | MTR_5g010430 | 58.571 | 140 | 56 | 1 | 6 | 145 | 1 | 138 | 1.92e-64 | 193 |
| MS.gene018706.t1 | MTR_2g028670 | 58.394 | 137 | 57 | 0 | 10 | 146 | 3 | 139 | 1.96e-63 | 191 |
| MS.gene018706.t1 | MTR_8g098470 | 59.559 | 136 | 53 | 1 | 10 | 145 | 3 | 136 | 1.13e-61 | 186 |
| MS.gene018706.t1 | MTR_8g088210 | 58.088 | 136 | 57 | 0 | 10 | 145 | 3 | 138 | 1.83e-61 | 186 |
| MS.gene018706.t1 | MTR_8g088210 | 57.252 | 131 | 56 | 0 | 15 | 145 | 1 | 131 | 6.87e-58 | 176 |
| MS.gene018706.t1 | MTR_1g101190 | 59.559 | 136 | 53 | 1 | 10 | 145 | 464 | 597 | 1.61e-56 | 186 |
| MS.gene018706.t1 | MTR_4g073590 | 68.889 | 90 | 28 | 0 | 11 | 100 | 8 | 97 | 1.54e-44 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018706.t1 | AT2G31200 | 77.397 | 146 | 33 | 0 | 1 | 146 | 1 | 146 | 1.90e-86 | 249 |
| MS.gene018706.t1 | AT3G46010 | 60.000 | 145 | 57 | 1 | 3 | 146 | 6 | 150 | 3.14e-68 | 203 |
| MS.gene018706.t1 | AT3G46010 | 60.584 | 137 | 54 | 0 | 10 | 146 | 3 | 139 | 1.96e-67 | 201 |
| MS.gene018706.t1 | AT5G59890 | 60.584 | 137 | 54 | 0 | 10 | 146 | 3 | 139 | 2.07e-67 | 201 |
| MS.gene018706.t1 | AT4G34970 | 62.857 | 140 | 52 | 0 | 6 | 145 | 1 | 140 | 2.57e-66 | 198 |
| MS.gene018706.t1 | AT2G16700 | 62.222 | 135 | 51 | 0 | 11 | 145 | 8 | 142 | 4.58e-64 | 192 |
| MS.gene018706.t1 | AT4G25590 | 61.765 | 136 | 50 | 1 | 10 | 145 | 3 | 136 | 5.18e-64 | 192 |
| MS.gene018706.t1 | AT5G59890 | 59.848 | 132 | 53 | 0 | 15 | 146 | 1 | 132 | 8.14e-64 | 191 |
| MS.gene018706.t1 | AT5G59890 | 59.848 | 132 | 53 | 0 | 15 | 146 | 1 | 132 | 8.14e-64 | 191 |
| MS.gene018706.t1 | AT4G00680 | 56.115 | 139 | 61 | 0 | 8 | 146 | 11 | 149 | 1.24e-63 | 191 |
| MS.gene018706.t1 | AT4G00680 | 56.934 | 137 | 59 | 0 | 10 | 146 | 3 | 139 | 1.46e-63 | 191 |
| MS.gene018706.t1 | AT1G01750 | 56.835 | 139 | 60 | 0 | 8 | 146 | 6 | 144 | 1.59e-63 | 191 |
| MS.gene018706.t1 | AT1G01750 | 57.664 | 137 | 58 | 0 | 10 | 146 | 3 | 139 | 1.84e-63 | 191 |
| MS.gene018706.t1 | AT3G46000 | 61.314 | 137 | 51 | 1 | 10 | 146 | 3 | 137 | 2.04e-63 | 191 |
| MS.gene018706.t1 | AT3G46000 | 61.314 | 137 | 51 | 1 | 10 | 146 | 22 | 156 | 2.30e-63 | 191 |
| MS.gene018706.t1 | AT5G59880 | 56.934 | 137 | 59 | 0 | 10 | 146 | 3 | 139 | 6.43e-63 | 189 |
| MS.gene018706.t1 | AT2G16700 | 62.595 | 131 | 49 | 0 | 15 | 145 | 1 | 131 | 7.35e-62 | 186 |
| MS.gene018706.t1 | AT2G16700 | 62.595 | 131 | 49 | 0 | 15 | 145 | 1 | 131 | 7.35e-62 | 186 |
| MS.gene018706.t1 | AT5G52360 | 60.294 | 136 | 52 | 1 | 10 | 145 | 3 | 136 | 2.73e-61 | 185 |
| MS.gene018706.t1 | AT4G25590 | 61.069 | 131 | 49 | 1 | 15 | 145 | 1 | 129 | 1.53e-60 | 183 |
| MS.gene018706.t1 | AT3G46000 | 60.606 | 132 | 50 | 1 | 15 | 146 | 1 | 130 | 6.22e-60 | 181 |
| MS.gene018706.t1 | AT5G59880 | 51.095 | 137 | 52 | 1 | 10 | 146 | 3 | 124 | 9.64e-51 | 158 |
| MS.gene018706.t1 | AT2G16700 | 55.882 | 102 | 45 | 0 | 11 | 112 | 8 | 109 | 4.90e-38 | 125 |
| MS.gene018706.t1 | AT3G45990 | 44.853 | 136 | 64 | 3 | 15 | 144 | 1 | 131 | 7.26e-37 | 123 |
Find 35 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATCGTTATGTCATCTTTA+AGG | 0.121401 | 7.1:+10922588 | MS.gene018706:CDS |
| TTTCTGTGGGGTCAGTAGCC+TGG | 0.377595 | 7.1:-10931006 | None:intergenic |
| GGCTACTGACCCCACAGAAA+TGG | 0.409200 | 7.1:+10931009 | MS.gene018706:CDS |
| TTAAGGTTGATGAAAAGAAA+AGG | 0.433478 | 7.1:+10922605 | MS.gene018706:CDS |
| GCACGGATTCGAGACGTTGA+AGG | 0.442019 | 7.1:-10930917 | None:intergenic |
| TAAGGTTGATGAAAAGAAAA+GGG | 0.442046 | 7.1:+10922606 | MS.gene018706:CDS |
| CAGTAGCCTGGATTTCATAA+TGG | 0.449862 | 7.1:-10930994 | None:intergenic |
| TTACAGCCAAATGCTTCATC+TGG | 0.474975 | 7.1:+10922508 | MS.gene018706:intron |
| GCAAAATATTCTTCATTGCA+TGG | 0.476923 | 7.1:+10922758 | MS.gene018706:CDS |
| AGTTGTGGCATAGAGCATCT+TGG | 0.477388 | 7.1:-10930939 | None:intergenic |
| AGGTTTAGACGTGAGCTAGA+TGG | 0.488623 | 7.1:+10930968 | MS.gene018706:CDS |
| TGGTGTCCATTATGAAATCC+AGG | 0.494209 | 7.1:+10930988 | MS.gene018706:CDS |
| TCTAAACCTTTCTTTAGTTG+TGG | 0.494754 | 7.1:-10930954 | None:intergenic |
| GCCAAATGCTTCATCTGGCA+TGG | 0.501914 | 7.1:+10922513 | MS.gene018706:intron |
| GCATATCTGCAATCATTCTC+AGG | 0.507662 | 7.1:-10922691 | None:intergenic |
| CCCATGCCAGATGAAGCATT+TGG | 0.521485 | 7.1:-10922514 | None:intergenic |
| GAGGTTGTGGTTGAGAAGAC+TGG | 0.525287 | 7.1:+10922628 | MS.gene018706:CDS |
| TGTCTTTCAGAGGTCTCAGC+CGG | 0.525354 | 7.1:+10922354 | MS.gene018706:CDS |
| ACTTCAAGATCCATTTCTGT+GGG | 0.536095 | 7.1:-10931019 | None:intergenic |
| AAATCGTCATAACTCTCAGC+AGG | 0.549468 | 7.1:-10922655 | None:intergenic |
| CACTTCAAGATCCATTTCTG+TGG | 0.558716 | 7.1:-10931020 | None:intergenic |
| CATTGACATATCAGTGTGCT+CGG | 0.558788 | 7.1:-10931051 | None:intergenic |
| TCTATGCCACAACTAAAGAA+AGG | 0.562569 | 7.1:+10930948 | MS.gene018706:CDS |
| CCAAATGCTTCATCTGGCAT+GGG | 0.575461 | 7.1:+10922514 | MS.gene018706:CDS |
| GTCTTTCAGAGGTCTCAGCC+GGG | 0.578693 | 7.1:+10922355 | MS.gene018706:CDS |
| AAATGGATCTTGAAGTGCTG+AGG | 0.581724 | 7.1:+10931026 | MS.gene018706:CDS |
| AATGGATCTTGAAGTGCTGA+GGG | 0.603005 | 7.1:+10931027 | MS.gene018706:CDS |
| TGAAAAGAAAAGGGAGGTTG+TGG | 0.608416 | 7.1:+10922615 | MS.gene018706:CDS |
| TGGCATAGAGCATCTTGGCA+CGG | 0.613003 | 7.1:-10930934 | None:intergenic |
| GCTGAGACCTCTGAAAGACA+TGG | 0.632319 | 7.1:-10922351 | None:intergenic |
| GGTTGATGAAAAGAAAAGGG+AGG | 0.632508 | 7.1:+10922609 | MS.gene018706:CDS |
| ATCATTCTCAGGCAAAGACG+CGG | 0.642351 | 7.1:-10922680 | None:intergenic |
| CCTTAAAGATGACATAACGA+TGG | 0.653418 | 7.1:-10922588 | None:intergenic |
| GTTGTGGTTGAGAAGACTGG+TGG | 0.675505 | 7.1:+10922631 | MS.gene018706:CDS |
| CTTCAAGATCCATTTCTGTG+GGG | 0.730380 | 7.1:-10931018 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 10922353 | 10931063 | 10922353 | ID=MS.gene018706 |
| chr7.1 | mRNA | 10922353 | 10931063 | 10922353 | ID=MS.gene018706.t1;Parent=MS.gene018706 |
| chr7.1 | exon | 10922353 | 10922376 | 10922353 | ID=MS.gene018706.t1.exon1;Parent=MS.gene018706.t1 |
| chr7.1 | CDS | 10922353 | 10922376 | 10922353 | ID=cds.MS.gene018706.t1;Parent=MS.gene018706.t1 |
| chr7.1 | exon | 10922514 | 10922779 | 10922514 | ID=MS.gene018706.t1.exon2;Parent=MS.gene018706.t1 |
| chr7.1 | CDS | 10922514 | 10922779 | 10922514 | ID=cds.MS.gene018706.t1;Parent=MS.gene018706.t1 |
| chr7.1 | exon | 10930913 | 10931063 | 10930913 | ID=MS.gene018706.t1.exon3;Parent=MS.gene018706.t1 |
| chr7.1 | CDS | 10930913 | 10931063 | 10930913 | ID=cds.MS.gene018706.t1;Parent=MS.gene018706.t1 |
| Gene Sequence |
| Protein sequence |
