AlfalfaGEDB | MS.gene27898

M. sativa cultivar XinJiangDaYe / MS.gene27898

KEGG: K05765
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005856 GO:0007015 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0015629 GO:0016043 GO:0022411 GO:0030042 GO:0032984 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0051261 GO:0071840 GO:0097435 GO:0110165
PFAM: PF00241.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27898.t1	XP_003629907.1	97.7	132	3	0	1	132	8	139	1.90E-67	265

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27898.t1	Q9FVI1	84.0	131	21	0	1	131	8	138	9.4e-60	230.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27898.t1	A0A072TTE6	97.7	132	3	0	1	132	1	132	1.3e-67	265.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene037983	MS.gene27898	PPI
MS.gene037986	MS.gene27898	PPI
MS.gene47853	MS.gene27898	PPI
MS.gene037980	MS.gene27898	PPI
MS.gene063026	MS.gene27898	PPI
MS.gene87667	MS.gene27898	PPI
MS.gene020366	MS.gene27898	PPI
MS.gene037982	MS.gene27898	PPI
MS.gene058991	MS.gene27898	PPI
MS.gene003535	MS.gene27898	PPI
MS.gene02797	MS.gene27898	PPI
MS.gene057694	MS.gene27898	PPI
MS.gene003218	MS.gene27898	PPI
MS.gene018706	MS.gene27898	PPI
MS.gene023659	MS.gene27898	PPI
MS.gene34500	MS.gene27898	PPI
MS.gene24057	MS.gene27898	PPI
MS.gene27898	MS.gene23488	PPI
MS.gene037990	MS.gene27898	PPI
MS.gene97005	MS.gene27898	PPI
MS.gene41437	MS.gene27898	PPI
MS.gene27898	MS.gene34500	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27898.t1	MTR_8g088210	97.727	132	3	0	1	132	8	139	1.30e-95	271
MS.gene27898.t1	MTR_8g088210	97.727	132	3	0	1	132	1	132	3.08e-95	270
MS.gene27898.t1	MTR_2g028670	87.879	132	16	0	1	132	8	139	4.05e-88	252
MS.gene27898.t1	MTR_1g068950	78.030	132	29	0	1	132	8	139	1.60e-76	223
MS.gene27898.t1	MTR_8g098470	70.992	131	36	1	1	131	8	136	2.03e-69	205
MS.gene27898.t1	MTR_5g010430	67.176	131	41	1	1	131	10	138	1.70e-64	192
MS.gene27898.t1	MTR_1g101190	67.939	131	40	1	1	131	469	597	2.33e-60	195
MS.gene27898.t1	MTR_7g096890	58.333	132	55	0	1	132	15	146	8.10e-59	179
MS.gene27898.t1	MTR_4g073590	58.779	131	54	0	1	131	12	142	1.64e-55	170
MS.gene27898.t1	MTR_1g076170	56.061	132	58	0	1	132	16	147	1.88e-46	147
MS.gene27898.t1	MTR_4g073590	56.627	83	36	0	1	83	12	94	3.09e-31	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27898.t1	AT3G46010	79.545	132	27	0	1	132	19	150	5.49e-81	235
MS.gene27898.t1	AT3G46010	79.545	132	27	0	1	132	8	139	6.17e-81	234
MS.gene27898.t1	AT5G59880	81.061	132	25	0	1	132	8	139	5.07e-80	232
MS.gene27898.t1	AT5G59890	79.545	132	27	0	1	132	1	132	3.17e-78	227
MS.gene27898.t1	AT5G59890	79.545	132	27	0	1	132	1	132	3.17e-78	227
MS.gene27898.t1	AT5G59890	79.545	132	27	0	1	132	8	139	3.24e-78	227
MS.gene27898.t1	AT3G46000	76.515	132	29	1	1	132	8	137	2.79e-73	215
MS.gene27898.t1	AT3G46000	76.515	132	29	1	1	132	1	130	3.51e-73	214
MS.gene27898.t1	AT3G46000	76.515	132	29	1	1	132	27	156	4.57e-73	215
MS.gene27898.t1	AT1G01750	68.939	132	41	0	1	132	8	139	6.28e-68	201
MS.gene27898.t1	AT1G01750	68.939	132	41	0	1	132	13	144	7.89e-68	201
MS.gene27898.t1	AT4G00680	67.424	132	43	0	1	132	8	139	1.27e-67	201
MS.gene27898.t1	AT4G00680	67.424	132	43	0	1	132	18	149	2.16e-67	200
MS.gene27898.t1	AT4G25590	68.702	131	39	1	1	131	8	136	7.73e-66	196
MS.gene27898.t1	AT4G25590	68.702	131	39	1	1	131	1	129	1.23e-65	195
MS.gene27898.t1	AT5G52360	67.939	131	40	1	1	131	8	136	6.14e-65	194
MS.gene27898.t1	AT5G59880	69.697	132	25	1	1	132	8	124	8.69e-65	193
MS.gene27898.t1	AT2G31200	53.788	132	61	0	1	132	15	146	2.53e-54	167
MS.gene27898.t1	AT4G34970	54.198	131	60	0	1	131	10	140	2.92e-52	162
MS.gene27898.t1	AT2G16700	53.435	131	61	0	1	131	12	142	6.23e-51	159
MS.gene27898.t1	AT2G16700	53.435	131	61	0	1	131	1	131	7.07e-51	158
MS.gene27898.t1	AT2G16700	53.435	131	61	0	1	131	1	131	7.07e-51	158
MS.gene27898.t1	AT3G45990	58.993	139	44	4	1	132	1	133	1.97e-49	154
MS.gene27898.t1	AT2G16700	51.807	83	40	0	1	83	12	94	1.87e-27	98.6

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGACTGCAAGTTGAGGTTTA+TGG	0.271763	4.1:+12421913	MS.gene27898:CDS
CTCTCTCTTGAATCTATCTT+TGG	0.282496	4.1:-12422276	None:intergenic
CTTCTAACCCTTGATGTGTC+AGG	0.419707	4.1:-12422233	None:intergenic
GTTCATCATTTACAAGATTG+AGG	0.446804	4.1:+12421961	MS.gene27898:CDS
GCACTCATCAGCAGGAAGGC+AGG	0.460234	4.1:-12422050	None:intergenic
GCAACTGATCCTACTGAGAT+TGG	0.468027	4.1:+12422326	MS.gene27898:CDS
ACTGTTGGAGATCATTTAGT+TGG	0.469715	4.1:-12422372	None:intergenic
GAAGGTCTCCTGACACATCA+AGG	0.471655	4.1:+12422225	MS.gene27898:intron
ACCTCAACTTGCAGTCATCA+TGG	0.504088	4.1:-12421907	None:intergenic
AGATTCAAGAGAGAGCTTGA+TGG	0.510719	4.1:+12422284	MS.gene27898:CDS
GCATAGCGGCACTCATCAGC+AGG	0.511636	4.1:-12422058	None:intergenic
CAAGTCATTGTGGAGAAGCT+TGG	0.515016	4.1:+12421995	MS.gene27898:CDS
AAGGCAATGTTCCCAAAAGC+AGG	0.520698	4.1:+12422107	MS.gene27898:CDS
TTATGGAGCTCAAGGCGAAA+AGG	0.536997	4.1:+12421930	MS.gene27898:CDS
GATTATGAGTTTATGACTGA+AGG	0.546901	4.1:+12422088	MS.gene27898:CDS
GTTGAGGTTTATGGAGCTCA+AGG	0.554455	4.1:+12421922	MS.gene27898:CDS
AATTGTTCGTAACCTTCGAC+GGG	0.561631	4.1:-12422022	None:intergenic
AAATTGTTCGTAACCTTCGA+CGG	0.562726	4.1:-12422023	None:intergenic
TGGAGATCATTTAGTTGGCA+CGG	0.583240	4.1:-12422367	None:intergenic
AAGCTTGGCGAGCCCGTCGA+AGG	0.586923	4.1:+12422010	MS.gene27898:CDS
TCCATGATGACTGCAAGTTG+AGG	0.591374	4.1:+12421906	MS.gene27898:CDS
AAGGTCTCCTGACACATCAA+GGG	0.598732	4.1:+12422226	MS.gene27898:intron
AGCGGCACTCATCAGCAGGA+AGG	0.621421	4.1:-12422054	None:intergenic
ACATCAAGACCAATCTCAGT+AGG	0.622765	4.1:-12422335	None:intergenic
GAAACAGAAACAAGTCATTG+TGG	0.633781	4.1:+12421985	MS.gene27898:CDS
TCAAGGCGAAAAGGACACAC+AGG	0.692606	4.1:+12421939	MS.gene27898:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACATGATAGTTTCTTTTGA+AGG	+	chr4.1:12422207-12422226	MS.gene27898:intron	25.0%
!!	TATGAAAAAAATCCTGCTTT+TGG	-	chr4.1:12422122-12422141	None:intergenic	25.0%
!!!	ATGAAAAAAATCCTGCTTTT+GGG	-	chr4.1:12422121-12422140	None:intergenic	25.0%
	CATAATCAAAAACAGCATAG+CGG	-	chr4.1:12422075-12422094	None:intergenic	30.0%
	GATTATGAGTTTATGACTGA+AGG	+	chr4.1:12422088-12422107	MS.gene27898:CDS	30.0%
	GTTCATCATTTACAAGATTG+AGG	+	chr4.1:12421961-12421980	MS.gene27898:CDS	30.0%
	AAATTGTTCGTAACCTTCGA+CGG	-	chr4.1:12422026-12422045	None:intergenic	35.0%
	CTCTCTCTTGAATCTATCTT+TGG	-	chr4.1:12422279-12422298	None:intergenic	35.0%
	GAAACAGAAACAAGTCATTG+TGG	+	chr4.1:12421985-12422004	MS.gene27898:CDS	35.0%
!!!	GCAGGATTTTTTTCATAGCA+TGG	+	chr4.1:12422125-12422144	MS.gene27898:CDS	35.0%
	AATTGTTCGTAACCTTCGAC+GGG	-	chr4.1:12422025-12422044	None:intergenic	40.0%
	ACATCAAGACCAATCTCAGT+AGG	-	chr4.1:12422338-12422357	None:intergenic	40.0%
	AGATTCAAGAGAGAGCTTGA+TGG	+	chr4.1:12422284-12422303	MS.gene27898:CDS	40.0%
!	TGACTGCAAGTTGAGGTTTA+TGG	+	chr4.1:12421913-12421932	MS.gene27898:CDS	40.0%
!!	AGCATGGTGATGTTCTGTTT+TGG	+	chr4.1:12422141-12422160	MS.gene27898:intron	40.0%
	AAGGCAATGTTCCCAAAAGC+AGG	+	chr4.1:12422107-12422126	MS.gene27898:CDS	45.0%
	AAGGTCTCCTGACACATCAA+GGG	+	chr4.1:12422226-12422245	MS.gene27898:intron	45.0%
	ACCGATGCTACAACAGTAAC+TGG	-	chr4.1:12422171-12422190	None:intergenic	45.0%
	ACCTCAACTTGCAGTCATCA+TGG	-	chr4.1:12421910-12421929	None:intergenic	45.0%
	CAAGTCATTGTGGAGAAGCT+TGG	+	chr4.1:12421995-12422014	MS.gene27898:CDS	45.0%
	CTTCTAACCCTTGATGTGTC+AGG	-	chr4.1:12422236-12422255	None:intergenic	45.0%
	GCAACTGATCCTACTGAGAT+TGG	+	chr4.1:12422326-12422345	MS.gene27898:CDS	45.0%
	GCCAGTTACTGTTGTAGCAT+CGG	+	chr4.1:12422167-12422186	MS.gene27898:intron	45.0%
	GTTGAGGTTTATGGAGCTCA+AGG	+	chr4.1:12421922-12421941	MS.gene27898:CDS	45.0%
	TCCATGATGACTGCAAGTTG+AGG	+	chr4.1:12421906-12421925	MS.gene27898:CDS	45.0%
	TTATGGAGCTCAAGGCGAAA+AGG	+	chr4.1:12421930-12421949	MS.gene27898:CDS	45.0%
	GAAGGTCTCCTGACACATCA+AGG	+	chr4.1:12422225-12422244	MS.gene27898:intron	50.0%
	TCAAGGCGAAAAGGACACAC+AGG	+	chr4.1:12421939-12421958	MS.gene27898:CDS	50.0%
	AGCGGCACTCATCAGCAGGA+AGG	-	chr4.1:12422057-12422076	None:intergenic	60.0%
	GCACTCATCAGCAGGAAGGC+AGG	-	chr4.1:12422053-12422072	None:intergenic	60.0%
	GCATAGCGGCACTCATCAGC+AGG	-	chr4.1:12422061-12422080	None:intergenic	60.0%
	AAGCTTGGCGAGCCCGTCGA+AGG	+	chr4.1:12422010-12422029	MS.gene27898:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	12421899	12422379	12421899	ID=MS.gene27898
chr4.1	mRNA	12421899	12422379	12421899	ID=MS.gene27898.t1;Parent=MS.gene27898
chr4.1	exon	12421899	12422146	12421899	ID=MS.gene27898.t1.exon1;Parent=MS.gene27898.t1
chr4.1	CDS	12421899	12422146	12421899	ID=cds.MS.gene27898.t1;Parent=MS.gene27898.t1
chr4.1	exon	12422229	12422379	12422229	ID=MS.gene27898.t1.exon2;Parent=MS.gene27898.t1
chr4.1	CDS	12422229	12422379	12422229	ID=cds.MS.gene27898.t1;Parent=MS.gene27898.t1

Gene Sequence

>MS.gene27898

ATGGCGGTCCATGATGACTGCAAGTTGAGGTTTATGGAGCTCAAGGCGAAAAGGACACACAGGTTCATCATTTACAAGATTGAGGAGAAACAGAAACAAGTCATTGTGGAGAAGCTTGGCGAGCCCGTCGAAGGTTACGAACAATTTGCTGCCTGCCTTCCTGCTGATGAGTGCCGCTATGCTGTTTTTGATTATGAGTTTATGACTGAAGGCAATGTTCCCAAAAGCAGGATTTTTTTCATAGCATGGTGATGTTCTGTTTTGGTTTGCCAGTTACTGTTGTAGCATCGGTATTAAATTTTTATCTAAACATGATAGTTTCTTTTGAAGGTCTCCTGACACATCAAGGGTTAGAAGCAAGATGATTTATGCAAGCTCCAAAGATAGATTCAAGAGAGAGCTTGATGGTATTCAAATTGAGTTGCAAGCAACTGATCCTACTGAGATTGGTCTTGATGTGTTCAAAAGCCGTGCCAACTAA

Protein sequence

>MS.gene27898.t1

MAVHDDCKLRFMELKAKRTHRFIIYKIEEKQKQVIVEKLGEPVEGYEQFAACLPADECRYAVFDYEFMTEGNVPKSRIFFIAWSPDTSRVRSKMIYASSKDRFKRELDGIQIELQATDPTEIGLDVFKSRAN