AlfalfaGEDB | MS.gene037980

M. sativa cultivar XinJiangDaYe / MS.gene037980

KEGG: K05765
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005856 GO:0007015 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0015629 GO:0016043 GO:0022411 GO:0030042 GO:0032984 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0051261 GO:0071840 GO:0097435 GO:0110165
PFAM: PF00241.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037980.t1	XP_013468343.1	97.8	138	3	0	34	171	2	139	2.30E-70	275

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037980.t1	Q9ZSK3	81.9	138	25	0	34	171	2	139	1.2e-62	240.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037980.t1	G7ZZK8	97.8	138	3	0	34	171	2	139	1.7e-70	275.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene037983	MS.gene037980	PPI
MS.gene037980	MS.gene057694	PPI
MS.gene037980	MS.gene34500	PPI
MS.gene037980	MS.gene97005	PPI
MS.gene87667	MS.gene037980	PPI
MS.gene037980	MS.gene47853	PPI
MS.gene037980	MS.gene27898	PPI
MS.gene003535	MS.gene037980	PPI
MS.gene003218	MS.gene037980	PPI
MS.gene037980	MS.gene037982	PPI
MS.gene037980	MS.gene02797	PPI
MS.gene037980	MS.gene037990	PPI
MS.gene037980	MS.gene24057	PPI
MS.gene41437	MS.gene037980	PPI
MS.gene037980	MS.gene063026	PPI
MS.gene018706	MS.gene037980	PPI
MS.gene023659	MS.gene037980	PPI
MS.gene037980	MS.gene020366	PPI
MS.gene037980	MS.gene058991	PPI
MS.gene037980	MS.gene23488	PPI
MS.gene037980	MS.gene037986	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037980.t1	MTR_1g068950	97.826	138	3	0	34	171	2	139	1.61e-97	278
MS.gene037980.t1	MTR_2g028670	78.261	138	30	0	34	171	2	139	3.20e-81	236
MS.gene037980.t1	MTR_8g088210	76.087	138	33	0	34	171	2	139	7.10e-78	228
MS.gene037980.t1	MTR_5g010430	74.286	140	34	1	31	170	1	138	9.13e-77	225
MS.gene037980.t1	MTR_8g098470	71.533	137	37	1	34	170	2	136	2.54e-73	216
MS.gene037980.t1	MTR_8g088210	75.000	132	33	0	40	171	1	132	5.48e-73	216
MS.gene037980.t1	MTR_1g101190	72.464	138	36	1	32	169	461	596	1.30e-67	216
MS.gene037980.t1	MTR_7g096890	59.574	141	57	0	31	171	6	146	3.98e-65	196
MS.gene037980.t1	MTR_1g076170	61.594	138	53	0	34	171	10	147	4.70e-59	181
MS.gene037980.t1	MTR_4g073590	60.000	135	54	0	36	170	8	142	6.03e-58	178
MS.gene037980.t1	MTR_4g073590	60.920	87	34	0	36	122	8	94	2.19e-35	119

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037980.t1	AT5G59890	81.884	138	25	0	34	171	2	139	2.26e-84	244
MS.gene037980.t1	AT3G46010	81.884	138	25	0	34	171	13	150	8.03e-84	244
MS.gene037980.t1	AT3G46010	81.884	138	25	0	34	171	2	139	1.66e-83	243
MS.gene037980.t1	AT5G59890	81.061	132	25	0	40	171	1	132	1.48e-79	232
MS.gene037980.t1	AT5G59890	81.061	132	25	0	40	171	1	132	1.48e-79	232
MS.gene037980.t1	AT1G01750	72.917	144	39	0	28	171	1	144	7.88e-79	231
MS.gene037980.t1	AT4G00680	72.917	144	38	1	28	171	7	149	1.58e-78	230
MS.gene037980.t1	AT5G59880	74.638	138	35	0	34	171	2	139	9.57e-78	228
MS.gene037980.t1	AT4G00680	73.913	138	36	0	34	171	2	139	1.33e-77	228
MS.gene037980.t1	AT1G01750	74.638	138	35	0	34	171	2	139	4.02e-77	226
MS.gene037980.t1	AT3G46000	76.812	138	30	1	34	171	2	137	2.25e-76	224
MS.gene037980.t1	AT3G46000	76.812	138	30	1	34	171	21	156	4.76e-76	224
MS.gene037980.t1	AT4G25590	75.182	137	32	1	34	170	2	136	6.56e-74	218
MS.gene037980.t1	AT5G52360	75.182	137	32	1	34	170	2	136	7.37e-72	213
MS.gene037980.t1	AT3G46000	75.758	132	30	1	40	171	1	130	1.66e-71	212
MS.gene037980.t1	AT4G25590	74.046	131	32	1	40	170	1	129	5.57e-69	205
MS.gene037980.t1	AT5G59880	63.768	138	35	1	34	171	2	124	1.26e-62	189
MS.gene037980.t1	AT2G31200	58.156	141	59	0	31	171	6	146	1.43e-61	187
MS.gene037980.t1	AT2G16700	53.676	136	63	0	36	171	8	143	5.52e-54	168
MS.gene037980.t1	AT4G34970	53.285	137	64	0	35	171	5	141	5.78e-54	168
MS.gene037980.t1	AT2G16700	53.788	132	61	0	40	171	1	132	8.44e-52	162
MS.gene037980.t1	AT2G16700	53.788	132	61	0	40	171	1	132	8.44e-52	162
MS.gene037980.t1	AT3G45990	51.449	138	56	3	40	171	1	133	3.09e-43	140
MS.gene037980.t1	AT2G16700	49.020	102	52	0	36	137	8	109	1.58e-32	112

Find 30 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTACCCTTGAAATATC+AGG	0.195342	1.4:-55161594	None:intergenic
GTATATCATTTACAAGATTA+AGG	0.294112	1.4:+55161292	MS.gene037980:CDS
TACACTAATCTAATGGTAAT+GGG	0.308098	1.4:+55161101	None:intergenic
CTACACTAATCTAATGGTAA+TGG	0.419474	1.4:+55161100	None:intergenic
TTTATGCTAGCTCCAAAGAT+AGG	0.430442	1.4:+55161625	MS.gene037980:CDS
AAATCATCGTAACCATTTGC+AGG	0.435601	1.4:-55161353	None:intergenic
GAAGGTGCCCTGATATTTCA+AGG	0.462729	1.4:+55161586	MS.gene037980:intron
TTACAGGCGAATGCAGCATC+AGG	0.463408	1.4:+55161206	MS.gene037980:CDS
GCTCCAAAGATAGGTTCAAA+AGG	0.469123	1.4:+55161634	MS.gene037980:CDS
GCATATCGACACTCGTCGGC+AGG	0.482779	1.4:-55161389	None:intergenic
TGTGGCGTTGAAAGTGTGTA+TGG	0.483413	1.4:+55161142	MS.gene037980:CDS
GGTTCAAAAGGGAGCTTGAT+GGG	0.491075	1.4:+55161646	MS.gene037980:CDS
ACATCAGATGCATCAACAAA+AGG	0.502015	1.4:-55161173	None:intergenic
GGCGAATGCAGCATCAGGTA+TGG	0.502868	1.4:+55161211	MS.gene037980:CDS
GCCCTGATATTTCAAGGGTA+AGG	0.520257	1.4:+55161592	MS.gene037980:CDS
CAAGTTGTTGTTGACAAGGT+TGG	0.532386	1.4:+55161326	MS.gene037980:CDS
CTCCAAAGATAGGTTCAAAA+GGG	0.546134	1.4:+55161635	MS.gene037980:CDS
AGCTACTGATCCTACTGAGA+TGG	0.549628	1.4:+55161686	MS.gene037980:CDS
AGGTTCAAAAGGGAGCTTGA+TGG	0.551429	1.4:+55161645	MS.gene037980:CDS
GTTGTTAGTATGCTTGTTTG+TGG	0.553370	1.4:+55161124	MS.gene037980:CDS
TCCTTACCCTTGAAATATCA+GGG	0.558692	1.4:-55161593	None:intergenic
AAGGTTGGTGATCCTGCAAA+TGG	0.580318	1.4:+55161341	MS.gene037980:CDS
GAAGCAAGTTGTTGTTGACA+AGG	0.580658	1.4:+55161322	MS.gene037980:CDS
AAGGTGCCCTGATATTTCAA+GGG	0.592018	1.4:+55161587	MS.gene037980:intron
CTTGTAAATGATATACCGAT+AGG	0.592223	1.4:-55161285	None:intergenic
ACATCAAGATCCATCTCAGT+AGG	0.597944	1.4:-55161696	None:intergenic
AACAGCATATCGACACTCGT+CGG	0.602599	1.4:-55161393	None:intergenic
TCAAGGCGAAGAGGACCTAT+CGG	0.606898	1.4:+55161270	MS.gene037980:CDS
ACACTAATCTAATGGTAATG+GGG	0.642233	1.4:+55161102	None:intergenic
TCACAAAAGATTAATTGACA+CGG	0.694452	1.4:-55161728	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAATCAATCATAAAAAA+TGG	-	chr1.4:55161536-55161555	None:intergenic	10.0%
!!	GTATATCATTTACAAGATTA+AGG	+	chr1.4:55161292-55161311	MS.gene037980:CDS	20.0%
!	AAGCAACAAAATGATATACT+CGG	-	chr1.4:55161560-55161579	None:intergenic	25.0%
!	CAACAGTTAACTTATAGATT+TGG	+	chr1.4:55161505-55161524	MS.gene037980:intron	25.0%
!!	TGAATGCAAGTTAAAGTTTT+TGG	+	chr1.4:55161244-55161263	MS.gene037980:CDS	25.0%
!!	TGATGTTTGTGAAATTTTAC+AGG	+	chr1.4:55161190-55161209	MS.gene037980:CDS	25.0%
!!!	ATTTTGTTGCTTTTGTTTGA+AGG	+	chr1.4:55161568-55161587	MS.gene037980:intron	25.0%
	AGCAACAAAATGATATACTC+GGG	-	chr1.4:55161559-55161578	None:intergenic	30.0%
	CTTGTAAATGATATACCGAT+AGG	-	chr1.4:55161288-55161307	None:intergenic	30.0%
!!	TGAAGAAAATTCTGCTTTTC+TGG	-	chr1.4:55161451-55161470	None:intergenic	30.0%
!!!	TGATTTTGATTTTGTCACTG+AGG	+	chr1.4:55161418-55161437	MS.gene037980:CDS	30.0%
	AAATCATCGTAACCATTTGC+AGG	-	chr1.4:55161356-55161375	None:intergenic	35.0%
	ACATCAGATGCATCAACAAA+AGG	-	chr1.4:55161176-55161195	None:intergenic	35.0%
	CTCCAAAGATAGGTTCAAAA+GGG	+	chr1.4:55161635-55161654	MS.gene037980:CDS	35.0%
	GTTGTTAGTATGCTTGTTTG+TGG	+	chr1.4:55161124-55161143	MS.gene037980:CDS	35.0%
	TCCTTACCCTTGAAATATCA+GGG	-	chr1.4:55161596-55161615	None:intergenic	35.0%
	TTTATGCTAGCTCCAAAGAT+AGG	+	chr1.4:55161625-55161644	MS.gene037980:CDS	35.0%
!	GCAGAATTTTCTTCATAGCT+TGG	+	chr1.4:55161456-55161475	MS.gene037980:CDS	35.0%
!!!	GCTTGGTAATGTCAACTTTT+TGG	+	chr1.4:55161473-55161492	MS.gene037980:intron	35.0%
!!!	GTTAAAGTTTTTGGAGCTCA+AGG	+	chr1.4:55161253-55161272	MS.gene037980:CDS	35.0%
	ACATCAAGATCCATCTCAGT+AGG	-	chr1.4:55161699-55161718	None:intergenic	40.0%
	CTCCTTACCCTTGAAATATC+AGG	-	chr1.4:55161597-55161616	None:intergenic	40.0%
	GCTCCAAAGATAGGTTCAAA+AGG	+	chr1.4:55161634-55161653	MS.gene037980:CDS	40.0%
!	AAGGTGCCCTGATATTTCAA+GGG	+	chr1.4:55161587-55161606	MS.gene037980:intron	40.0%
!	CAAGTTGTTGTTGACAAGGT+TGG	+	chr1.4:55161326-55161345	MS.gene037980:CDS	40.0%
!	CTCCCTTTTGAACCTATCTT+TGG	-	chr1.4:55161640-55161659	None:intergenic	40.0%
!	GAAGCAAGTTGTTGTTGACA+AGG	+	chr1.4:55161322-55161341	MS.gene037980:CDS	40.0%
	AACAGCATATCGACACTCGT+CGG	-	chr1.4:55161396-55161415	None:intergenic	45.0%
	AGCTACTGATCCTACTGAGA+TGG	+	chr1.4:55161686-55161705	MS.gene037980:CDS	45.0%
	AGGTTCAAAAGGGAGCTTGA+TGG	+	chr1.4:55161645-55161664	MS.gene037980:CDS	45.0%
	GCCCTGATATTTCAAGGGTA+AGG	+	chr1.4:55161592-55161611	MS.gene037980:CDS	45.0%
	GGTTCAAAAGGGAGCTTGAT+GGG	+	chr1.4:55161646-55161665	MS.gene037980:CDS	45.0%
!	TGTGGCGTTGAAAGTGTGTA+TGG	+	chr1.4:55161142-55161161	MS.gene037980:CDS	45.0%
!!	AAGGTTGGTGATCCTGCAAA+TGG	+	chr1.4:55161341-55161360	MS.gene037980:CDS	45.0%
!!	GAAGGTGCCCTGATATTTCA+AGG	+	chr1.4:55161586-55161605	MS.gene037980:intron	45.0%
	TCAAGGCGAAGAGGACCTAT+CGG	+	chr1.4:55161270-55161289	MS.gene037980:CDS	50.0%
	TTACAGGCGAATGCAGCATC+AGG	+	chr1.4:55161206-55161225	MS.gene037980:CDS	50.0%
!!!	TTTTGGAGCTCAAGGCGAAG+AGG	+	chr1.4:55161261-55161280	MS.gene037980:CDS	50.0%
	GGCGAATGCAGCATCAGGTA+TGG	+	chr1.4:55161211-55161230	MS.gene037980:CDS	55.0%
	GCATATCGACACTCGTCGGC+AGG	-	chr1.4:55161392-55161411	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	55161113	55161740	55161113	ID=MS.gene037980
chr1.4	mRNA	55161113	55161740	55161113	ID=MS.gene037980.t1;Parent=MS.gene037980
chr1.4	exon	55161113	55161477	55161113	ID=MS.gene037980.t1.exon1;Parent=MS.gene037980.t1
chr1.4	CDS	55161113	55161477	55161113	ID=cds.MS.gene037980.t1;Parent=MS.gene037980.t1
chr1.4	exon	55161590	55161740	55161590	ID=MS.gene037980.t1.exon2;Parent=MS.gene037980.t1
chr1.4	CDS	55161590	55161740	55161590	ID=cds.MS.gene037980.t1;Parent=MS.gene037980.t1

Gene Sequence

>MS.gene037980

ATGGTAATGGGGTTGTTAGTATGCTTGTTTGTGGCGTTGAAAGTGTGTATGGAGAAATTTCCTTTTGTTGATGCATCTGATGTTTGTGAAATTTTACAGGCGAATGCAGCATCAGGTATGGATGTGAATGATGAATGCAAGTTAAAGTTTTTGGAGCTCAAGGCGAAGAGGACCTATCGGTATATCATTTACAAGATTAAGGTGAAGCAGAAGCAAGTTGTTGTTGACAAGGTTGGTGATCCTGCAAATGGTTACGATGATTTCACTGCTAATCTTCCTGCCGACGAGTGTCGATATGCTGTTTATGATTTTGATTTTGTCACTGAGGAGAATTGCCAGAAAAGCAGAATTTTCTTCATAGCTTGGTAATGTCAACTTTTTGGATCTTAAAGCAACAGTTAACTTATAGATTTGGATATTCCATTTTTTATGATTGATTTTAACCCGAGTATATCATTTTGTTGCTTTTGTTTGAAGGTGCCCTGATATTTCAAGGGTAAGGAGCAAGATGATTTATGCTAGCTCCAAAGATAGGTTCAAAAGGGAGCTTGATGGGATTCAAGTTGAGTTGCAAGCTACTGATCCTACTGAGATGGATCTTGATGTGTTCAAAAGCCGTGTCAATTAA

Protein sequence

>MS.gene037980.t1

MVMGLLVCLFVALKVCMEKFPFVDASDVCEILQANAASGMDVNDECKLKFLELKAKRTYRYIIYKIKVKQKQVVVDKVGDPANGYDDFTANLPADECRYAVYDFDFVTEENCQKSRIFFIAWCPDISRVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFKSRVN