AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47853


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47853.t1 MTR_8g088210 97.842 139 3 0 1 139 1 139 3.14e-101 286
MS.gene47853.t1 MTR_8g088210 97.727 132 3 0 8 139 1 132 1.30e-95 271
MS.gene47853.t1 MTR_2g028670 88.489 139 16 0 1 139 1 139 7.75e-94 267
MS.gene47853.t1 MTR_1g068950 79.137 139 29 0 1 139 1 139 3.22e-82 238
MS.gene47853.t1 MTR_8g098470 72.464 138 36 1 1 138 1 136 5.24e-75 219
MS.gene47853.t1 MTR_5g010430 68.613 137 41 1 2 138 4 138 2.74e-69 205
MS.gene47853.t1 MTR_1g101190 69.343 137 40 1 2 138 463 597 7.02e-65 207
MS.gene47853.t1 MTR_7g096890 59.124 137 56 0 3 139 10 146 2.01e-62 188
MS.gene47853.t1 MTR_4g073590 58.519 135 56 0 4 138 8 142 1.75e-57 175
MS.gene47853.t1 MTR_1g076170 56.522 138 60 0 2 139 10 147 4.76e-50 157
MS.gene47853.t1 MTR_4g073590 56.322 87 38 0 4 90 8 94 2.90e-33 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47853.t1 AT3G46010 80.576 139 27 0 1 139 1 139 1.00e-86 249
MS.gene47853.t1 AT3G46010 80.435 138 27 0 2 139 13 150 6.11e-86 248
MS.gene47853.t1 AT5G59880 82.014 139 25 0 1 139 1 139 1.38e-85 246
MS.gene47853.t1 AT5G59890 80.576 139 27 0 1 139 1 139 4.32e-84 243
MS.gene47853.t1 AT3G46000 77.698 139 29 1 1 139 1 137 5.72e-79 229
MS.gene47853.t1 AT5G59890 79.545 132 27 0 8 139 1 132 1.60e-78 228
MS.gene47853.t1 AT5G59890 79.545 132 27 0 8 139 1 132 1.60e-78 228
MS.gene47853.t1 AT3G46000 76.978 139 30 1 1 139 20 156 5.05e-78 228
MS.gene47853.t1 AT3G46000 76.515 132 29 1 8 139 1 130 2.14e-73 215
MS.gene47853.t1 AT1G01750 69.784 139 42 0 1 139 1 139 3.54e-73 215
MS.gene47853.t1 AT4G00680 68.345 139 44 0 1 139 1 139 7.46e-73 214
MS.gene47853.t1 AT1G01750 69.565 138 42 0 2 139 7 144 2.84e-72 213
MS.gene47853.t1 AT4G00680 68.116 138 44 0 2 139 12 149 7.46e-72 212
MS.gene47853.t1 AT4G25590 70.290 138 39 1 1 138 1 136 2.55e-71 210
MS.gene47853.t1 AT5G52360 69.565 138 40 1 1 138 1 136 1.75e-70 208
MS.gene47853.t1 AT5G59880 71.223 139 25 1 1 139 1 124 2.26e-70 207
MS.gene47853.t1 AT4G25590 68.702 131 39 1 8 138 1 129 7.71e-66 196
MS.gene47853.t1 AT2G31200 54.745 137 62 0 3 139 10 146 2.35e-57 175
MS.gene47853.t1 AT4G34970 53.237 139 65 0 1 139 3 141 6.55e-55 169
MS.gene47853.t1 AT2G16700 53.333 135 63 0 4 138 8 142 6.20e-53 164
MS.gene47853.t1 AT2G16700 53.435 131 61 0 8 138 1 131 6.39e-51 158
MS.gene47853.t1 AT2G16700 53.435 131 61 0 8 138 1 131 6.39e-51 158
MS.gene47853.t1 AT3G45990 58.993 139 44 4 8 139 1 133 1.16e-49 155
MS.gene47853.t1 AT2G16700 51.724 87 42 0 4 90 8 94 1.74e-29 103

Find 31 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACTGCAAGTTGAGGTTTA+TGG 0.271763 4.4:+12813181 MS.gene47853:CDS
CTCTCTCTTGAATCTATCTT+TGG 0.282496 4.4:-12813544 None:intergenic
CTTCTAACCCTTGATGTGTC+AGG 0.419707 4.4:-12813501 None:intergenic
GTTCATCATTTACAAGATTG+AGG 0.446804 4.4:+12813229 MS.gene47853:CDS
GCAACTGATCCTACCGAGAT+TGG 0.447691 4.4:+12813594 MS.gene47853:CDS
GCACTCATCAGCAGGAAGGC+AGG 0.460234 4.4:-12813318 None:intergenic
ACTGTTGGAGATCATTTAGT+TGG 0.469715 4.4:-12813640 None:intergenic
GAAGGTCTCCTGACACATCA+AGG 0.471655 4.4:+12813493 MS.gene47853:intron
TTACAGGCAAATGCGGCATC+CGG 0.477481 4.4:+12813143 MS.gene47853:intron
GTCATCATGGACCGCCATAC+CGG 0.500234 4.4:-12813162 None:intergenic
ACCTCAACTTGCAGTCATCA+TGG 0.504088 4.4:-12813175 None:intergenic
AGATTCAAGAGAGAGCTTGA+TGG 0.510719 4.4:+12813552 MS.gene47853:CDS
GCATAGCGGCACTCATCAGC+AGG 0.511636 4.4:-12813326 None:intergenic
GGCAAATGCGGCATCCGGTA+TGG 0.514841 4.4:+12813148 MS.gene47853:intron
CAAGTCATTGTGGAGAAGCT+TGG 0.515016 4.4:+12813263 MS.gene47853:CDS
GAACACATCAAGACCAATCT+CGG 0.518975 4.4:-12813607 None:intergenic
AAGGCAATGTTCCCAAAAGC+AGG 0.520698 4.4:+12813375 MS.gene47853:CDS
TTATGGAGCTCAAGGCGAAA+AGG 0.536997 4.4:+12813198 MS.gene47853:CDS
GATTATGAGTTTATGACTGA+AGG 0.546901 4.4:+12813356 MS.gene47853:CDS
GTTGAGGTTTATGGAGCTCA+AGG 0.554455 4.4:+12813190 MS.gene47853:CDS
AATTGTTCGTAACCTTCGAC+GGG 0.561631 4.4:-12813290 None:intergenic
AAATTGTTCGTAACCTTCGA+CGG 0.562726 4.4:-12813291 None:intergenic
TGGAGATCATTTAGTTGGCA+CGG 0.583240 4.4:-12813635 None:intergenic
AAGCTTGGCGAGCCCGTCGA+AGG 0.586923 4.4:+12813278 MS.gene47853:CDS
TCCATGATGACTGCAAGTTG+AGG 0.591374 4.4:+12813174 MS.gene47853:CDS
AAGGTCTCCTGACACATCAA+GGG 0.598732 4.4:+12813494 MS.gene47853:intron
AGCGGCACTCATCAGCAGGA+AGG 0.621421 4.4:-12813322 None:intergenic
GAAACAGAAACAAGTCATTG+TGG 0.633781 4.4:+12813253 MS.gene47853:CDS
ACATCAAGACCAATCTCGGT+AGG 0.647816 4.4:-12813603 None:intergenic
AAATGCGGCATCCGGTATGG+CGG 0.649579 4.4:+12813151 MS.gene47853:CDS
TCAAGGCGAAAAGGACACAC+AGG 0.692606 4.4:+12813207 MS.gene47853:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCTTAAAAACCTAATTA+GGG + chr4.4:12812661-12812680 MS.gene47853:intron 15.0%
!! TAAATCTTAAAAACCTAATT+AGG + chr4.4:12812660-12812679 MS.gene47853:intron 15.0%
!! TACAATCATTTATTTATTTG+AGG + chr4.4:12813028-12813047 MS.gene47853:intron 15.0%
!!! ACTTATTTTTTTAGCTATTT+TGG + chr4.4:12812750-12812769 MS.gene47853:intron 15.0%
!!! CTTATTTTTTTAGCTATTTT+GGG + chr4.4:12812751-12812770 MS.gene47853:intron 15.0%
!!! TTATTTTTTTAGCTATTTTG+GGG + chr4.4:12812752-12812771 MS.gene47853:intron 15.0%
!!! TTGAATTAAATTTTATGTTG+TGG - chr4.4:12812515-12812534 None:intergenic 15.0%
!! AAATGATAAAGTAAACAGAA+CGG - chr4.4:12812225-12812244 None:intergenic 20.0%
!! ACAGTAATCAAATAATCATT+AGG - chr4.4:12812372-12812391 None:intergenic 20.0%
!! TAATAATTTAACTAAGCACT+TGG + chr4.4:12812695-12812714 MS.gene47853:intron 20.0%
!! TATATATGTTCTATTTGGAT+TGG - chr4.4:12812616-12812635 None:intergenic 20.0%
!! TTGCATATATATGTTCTATT+TGG - chr4.4:12812621-12812640 None:intergenic 20.0%
!! TTTATGTTGTGGTATAAAAA+TGG - chr4.4:12812504-12812523 None:intergenic 20.0%
!!! AATAAGTTGATCAAAACTTA+TGG + chr4.4:12812778-12812797 MS.gene47853:intron 20.0%
!!! ATTAGGTTTGCTTAAAAAAT+GGG - chr4.4:12812355-12812374 None:intergenic 20.0%
! AAAAATAAGGGAAACTTATG+TGG + chr4.4:12812910-12812929 MS.gene47853:intron 25.0%
! AATTAACCACTCAAAATCAT+AGG + chr4.4:12812402-12812421 MS.gene47853:intron 25.0%
! ACAAGCAGACATAATTTAAA+CGG - chr4.4:12813056-12813075 None:intergenic 25.0%
! AGAGAAAACACAATAAAACT+TGG - chr4.4:12812991-12813010 None:intergenic 25.0%
! GAAGTATGCTTCAAAAATAA+GGG + chr4.4:12812898-12812917 MS.gene47853:intron 25.0%
! TGAAGTATGCTTCAAAAATA+AGG + chr4.4:12812897-12812916 MS.gene47853:intron 25.0%
!! AACATGATAGTTTCTTTTGA+AGG + chr4.4:12813475-12813494 MS.gene47853:intron 25.0%
!! CATTAGGTTTGCTTAAAAAA+TGG - chr4.4:12812356-12812375 None:intergenic 25.0%
!! TATGAAAAAAATCCTGCTTT+TGG - chr4.4:12813390-12813409 None:intergenic 25.0%
!!! ATGAAAAAAATCCTGCTTTT+GGG - chr4.4:12813389-12813408 None:intergenic 25.0%
AAAACTTATGGATCTGTCAT+CGG + chr4.4:12812790-12812809 MS.gene47853:intron 30.0%
CAGTTTCAATCGAACTATTT+GGG + chr4.4:12813097-12813116 MS.gene47853:intron 30.0%
CATAATATTGACCTGAATGA+TGG - chr4.4:12812439-12812458 None:intergenic 30.0%
CATAATCAAAAACAGCATAG+CGG - chr4.4:12813343-12813362 None:intergenic 30.0%
GATTATGAGTTTATGACTGA+AGG + chr4.4:12813356-12813375 MS.gene47853:CDS 30.0%
GTTCATCATTTACAAGATTG+AGG + chr4.4:12813229-12813248 MS.gene47853:CDS 30.0%
TCAGCATTTAAGAAAGATGA+AGG - chr4.4:12812158-12812177 None:intergenic 30.0%
TTGAAGCATACTTCAATCTA+GGG - chr4.4:12812892-12812911 None:intergenic 30.0%
! TGTAATTTTACAGGCAAATG+CGG + chr4.4:12813136-12813155 MS.gene47853:intron 30.0%
! TTATGCGTCTGTAATTTTAC+AGG + chr4.4:12813127-12813146 MS.gene47853:intron 30.0%
! TTTGAAGCATACTTCAATCT+AGG - chr4.4:12812893-12812912 None:intergenic 30.0%
! TTTTCGTAATTCCATCATTC+AGG + chr4.4:12812425-12812444 MS.gene47853:intron 30.0%
!! AAAACCTCACTATTTTGAAG+GGG + chr4.4:12812946-12812965 MS.gene47853:intron 30.0%
!! GAAAACCTCACTATTTTGAA+GGG + chr4.4:12812945-12812964 MS.gene47853:intron 30.0%
!! TGAAAACCTCACTATTTTGA+AGG + chr4.4:12812944-12812963 MS.gene47853:intron 30.0%
AAATTGTTCGTAACCTTCGA+CGG - chr4.4:12813294-12813313 None:intergenic 35.0%
AATCCAAACAAGCCCTAATT+AGG - chr4.4:12812676-12812695 None:intergenic 35.0%
CCAAATAGTTCGATTGAAAC+TGG - chr4.4:12813099-12813118 None:intergenic 35.0%
CCAGTTTCAATCGAACTATT+TGG + chr4.4:12813096-12813115 MS.gene47853:intron 35.0%
CTCTCTCTTGAATCTATCTT+TGG - chr4.4:12813547-12813566 None:intergenic 35.0%
GAAACAGAAACAAGTCATTG+TGG + chr4.4:12813253-12813272 MS.gene47853:CDS 35.0%
GCTTATTTGAACTAATCCAG+CGG - chr4.4:12812594-12812613 None:intergenic 35.0%
TGAAGCATACTTCAATCTAG+GGG - chr4.4:12812891-12812910 None:intergenic 35.0%
! AAACCTAATTAGGGCTTGTT+TGG + chr4.4:12812670-12812689 MS.gene47853:intron 35.0%
! CGAAAACCTATGATTTTGAG+TGG - chr4.4:12812411-12812430 None:intergenic 35.0%
!!! GCAGGATTTTTTTCATAGCA+TGG + chr4.4:12813393-12813412 MS.gene47853:CDS 35.0%
AATTGTTCGTAACCTTCGAC+GGG - chr4.4:12813293-12813312 None:intergenic 40.0%
AGATTCAAGAGAGAGCTTGA+TGG + chr4.4:12813552-12813571 MS.gene47853:CDS 40.0%
GAACACATCAAGACCAATCT+CGG - chr4.4:12813610-12813629 None:intergenic 40.0%
GAGATCAAAATCAGATCTGC+AGG - chr4.4:12812249-12812268 None:intergenic 40.0%
TTCTCCCCTTCAAAATAGTG+AGG - chr4.4:12812953-12812972 None:intergenic 40.0%
! TGACTGCAAGTTGAGGTTTA+TGG + chr4.4:12813181-12813200 MS.gene47853:CDS 40.0%
!! AGCATGGTGATGTTCTGTTT+TGG + chr4.4:12813409-12813428 MS.gene47853:intron 40.0%
AAGGCAATGTTCCCAAAAGC+AGG + chr4.4:12813375-12813394 MS.gene47853:CDS 45.0%
AAGGTCTCCTGACACATCAA+GGG + chr4.4:12813494-12813513 MS.gene47853:intron 45.0%
ACATCAAGACCAATCTCGGT+AGG - chr4.4:12813606-12813625 None:intergenic 45.0%
ACCGATGCTACAACAGTAAC+TGG - chr4.4:12813439-12813458 None:intergenic 45.0%
ACCTCAACTTGCAGTCATCA+TGG - chr4.4:12813178-12813197 None:intergenic 45.0%
CAAGTCATTGTGGAGAAGCT+TGG + chr4.4:12813263-12813282 MS.gene47853:CDS 45.0%
CTTCTAACCCTTGATGTGTC+AGG - chr4.4:12813504-12813523 None:intergenic 45.0%
GCCAGTTACTGTTGTAGCAT+CGG + chr4.4:12813435-12813454 MS.gene47853:intron 45.0%
GTTGAGGTTTATGGAGCTCA+AGG + chr4.4:12813190-12813209 MS.gene47853:CDS 45.0%
TCAAATAGTTCCTTTGCCGC+TGG + chr4.4:12812575-12812594 MS.gene47853:intron 45.0%
TCCATGATGACTGCAAGTTG+AGG + chr4.4:12813174-12813193 MS.gene47853:CDS 45.0%
TTATGGAGCTCAAGGCGAAA+AGG + chr4.4:12813198-12813217 MS.gene47853:CDS 45.0%
TTGAACTAATCCAGCGGCAA+AGG - chr4.4:12812588-12812607 None:intergenic 45.0%
GAAGGTCTCCTGACACATCA+AGG + chr4.4:12813493-12813512 MS.gene47853:intron 50.0%
GCAACTGATCCTACCGAGAT+TGG + chr4.4:12813594-12813613 MS.gene47853:CDS 50.0%
TCAAGGCGAAAAGGACACAC+AGG + chr4.4:12813207-12813226 MS.gene47853:CDS 50.0%
TTACAGGCAAATGCGGCATC+CGG + chr4.4:12813143-12813162 MS.gene47853:intron 50.0%
AAATGCGGCATCCGGTATGG+CGG + chr4.4:12813151-12813170 MS.gene47853:CDS 55.0%
GTCATCATGGACCGCCATAC+CGG - chr4.4:12813165-12813184 None:intergenic 55.0%
AGCGGCACTCATCAGCAGGA+AGG - chr4.4:12813325-12813344 None:intergenic 60.0%
GCACTCATCAGCAGGAAGGC+AGG - chr4.4:12813321-12813340 None:intergenic 60.0%
GCATAGCGGCACTCATCAGC+AGG - chr4.4:12813329-12813348 None:intergenic 60.0%
GGCAAATGCGGCATCCGGTA+TGG + chr4.4:12813148-12813167 MS.gene47853:intron 60.0%
AAGCTTGGCGAGCCCGTCGA+AGG + chr4.4:12813278-12813297 MS.gene47853:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 12812139 12813647 12812139 ID=MS.gene47853
chr4.4 mRNA 12812139 12813647 12812139 ID=MS.gene47853.t1;Parent=MS.gene47853
chr4.4 exon 12812139 12812141 12812139 ID=MS.gene47853.t1.exon1;Parent=MS.gene47853.t1
chr4.4 CDS 12812139 12812141 12812139 ID=cds.MS.gene47853.t1;Parent=MS.gene47853.t1
chr4.4 exon 12813149 12813414 12813149 ID=MS.gene47853.t1.exon2;Parent=MS.gene47853.t1
chr4.4 CDS 12813149 12813414 12813149 ID=cds.MS.gene47853.t1;Parent=MS.gene47853.t1
chr4.4 exon 12813497 12813647 12813497 ID=MS.gene47853.t1.exon3;Parent=MS.gene47853.t1
chr4.4 CDS 12813497 12813647 12813497 ID=cds.MS.gene47853.t1;Parent=MS.gene47853.t1
Gene Sequence

>MS.gene47853

ATGGTCTGTTTCTTTTCCTTCATCTTTCTTAAATGCTGATTCAAATTACACTAACAATTTTCATTTTTTATTTTTTTTTAGTTCCGTTCTGTTTACTTTATCATTTACCTGCAGATCTGATTTTGATCTCAATTTCACTTTACTTTGTAGCATCACATAATTATCTTTGATCTTCTCTCTACTCAAATTGATTAGTTATGCGTTGAAGATGAACCCATTTTTTAAGCAAACCTAATGATTATTTGATTACTGTTATTAACTTTAATTAACCACTCAAAATCATAGGTTTTCGTAATTCCATCATTCAGGTCAATATTATGCGTGATTTTCAGCTTGTAGCATAAGCGATTCTTATGTATAAACCATTTTTATACCACAACATAAAATTTAATTCAATTATTTTTCTATAAACTATAAGCTATTTTCATAAGCTCTCTCAAATAGTTCCTTTGCCGCTGGATTAGTTCAAATAAGCCAATCCAAATAGAACATATATATGCAAATAAGATCTTTTCATTTTTTAAATCTTAAAAACCTAATTAGGGCTTGTTTGGATTAATAATTTAACTAAGCACTTGGTCGATAAAATATTCTATTGAGTTTAGTTAAACACTTATTTTTTTAGCTATTTTGGGGAGAAATAAGTTGATCAAAACTTATGGATCTGTCATCGGTTATTTTTATGATATCTAACGCTTTTATAAATCTTGTGTAATGATGTAATTATGCTGAAACAAGCTCTTTTTAATCCCCTAGATTGAAGTATGCTTCAAAAATAAGGGAAACTTATGTGGTAACGTTTGTATGAAAACCTCACTATTTTGAAGGGGAGAAGATATCAAATTAACTCCAAGTTTTATTGTGTTTTCTCTGTATTTTTGTTTTTGTTTACAATCATTTATTTATTTGAGGAACCGTTTAAATTATGTCTGCTTGTGTAGTCTTACTAGAAATTATCCAGTTTCAATCGAACTATTTGGGATGTCGATTATGCGTCTGTAATTTTACAGGCAAATGCGGCATCCGGTATGGCGGTCCATGATGACTGCAAGTTGAGGTTTATGGAGCTCAAGGCGAAAAGGACACACAGGTTCATCATTTACAAGATTGAGGAGAAACAGAAACAAGTCATTGTGGAGAAGCTTGGCGAGCCCGTCGAAGGTTACGAACAATTTGCTGCCTGCCTTCCTGCTGATGAGTGCCGCTATGCTGTTTTTGATTATGAGTTTATGACTGAAGGCAATGTTCCCAAAAGCAGGATTTTTTTCATAGCATGGTGATGTTCTGTTTTGGTTTGCCAGTTACTGTTGTAGCATCGGTATTAAATTTTTATCTAAACATGATAGTTTCTTTTGAAGGTCTCCTGACACATCAAGGGTTAGAAGCAAGATGATTTATGCAAGCTCCAAAGATAGATTCAAGAGAGAGCTTGATGGTATTCAAATTGAGTTGCAAGCAACTGATCCTACCGAGATTGGTCTTGATGTGTTCAAAAGCCGTGCCAACTAA

Protein sequence

>MS.gene47853.t1

MANAASGMAVHDDCKLRFMELKAKRTHRFIIYKIEEKQKQVIVEKLGEPVEGYEQFAACLPADECRYAVFDYEFMTEGNVPKSRIFFIAWSPDTSRVRSKMIYASSKDRFKRELDGIQIELQATDPTEIGLDVFKSRAN