AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87667


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87667.t1 MTR_1g068950 97.826 138 3 0 34 171 2 139 1.61e-97 278
MS.gene87667.t1 MTR_2g028670 78.261 138 30 0 34 171 2 139 3.20e-81 236
MS.gene87667.t1 MTR_8g088210 76.087 138 33 0 34 171 2 139 7.10e-78 228
MS.gene87667.t1 MTR_5g010430 74.286 140 34 1 31 170 1 138 9.13e-77 225
MS.gene87667.t1 MTR_8g098470 71.533 137 37 1 34 170 2 136 2.54e-73 216
MS.gene87667.t1 MTR_8g088210 75.000 132 33 0 40 171 1 132 5.48e-73 216
MS.gene87667.t1 MTR_1g101190 72.464 138 36 1 32 169 461 596 1.30e-67 216
MS.gene87667.t1 MTR_7g096890 59.574 141 57 0 31 171 6 146 3.98e-65 196
MS.gene87667.t1 MTR_1g076170 61.594 138 53 0 34 171 10 147 4.70e-59 181
MS.gene87667.t1 MTR_4g073590 60.000 135 54 0 36 170 8 142 6.03e-58 178
MS.gene87667.t1 MTR_4g073590 60.920 87 34 0 36 122 8 94 2.19e-35 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87667.t1 AT5G59890 81.884 138 25 0 34 171 2 139 2.26e-84 244
MS.gene87667.t1 AT3G46010 81.884 138 25 0 34 171 13 150 8.03e-84 244
MS.gene87667.t1 AT3G46010 81.884 138 25 0 34 171 2 139 1.66e-83 243
MS.gene87667.t1 AT5G59890 81.061 132 25 0 40 171 1 132 1.48e-79 232
MS.gene87667.t1 AT5G59890 81.061 132 25 0 40 171 1 132 1.48e-79 232
MS.gene87667.t1 AT1G01750 72.917 144 39 0 28 171 1 144 7.88e-79 231
MS.gene87667.t1 AT4G00680 72.917 144 38 1 28 171 7 149 1.58e-78 230
MS.gene87667.t1 AT5G59880 74.638 138 35 0 34 171 2 139 9.57e-78 228
MS.gene87667.t1 AT4G00680 73.913 138 36 0 34 171 2 139 1.33e-77 228
MS.gene87667.t1 AT1G01750 74.638 138 35 0 34 171 2 139 4.02e-77 226
MS.gene87667.t1 AT3G46000 76.812 138 30 1 34 171 2 137 2.25e-76 224
MS.gene87667.t1 AT3G46000 76.812 138 30 1 34 171 21 156 4.76e-76 224
MS.gene87667.t1 AT4G25590 75.182 137 32 1 34 170 2 136 6.56e-74 218
MS.gene87667.t1 AT5G52360 75.182 137 32 1 34 170 2 136 7.37e-72 213
MS.gene87667.t1 AT3G46000 75.758 132 30 1 40 171 1 130 1.66e-71 212
MS.gene87667.t1 AT4G25590 74.046 131 32 1 40 170 1 129 5.57e-69 205
MS.gene87667.t1 AT5G59880 63.768 138 35 1 34 171 2 124 1.26e-62 189
MS.gene87667.t1 AT2G31200 58.156 141 59 0 31 171 6 146 1.43e-61 187
MS.gene87667.t1 AT2G16700 53.676 136 63 0 36 171 8 143 5.52e-54 168
MS.gene87667.t1 AT4G34970 53.285 137 64 0 35 171 5 141 5.78e-54 168
MS.gene87667.t1 AT2G16700 53.788 132 61 0 40 171 1 132 8.44e-52 162
MS.gene87667.t1 AT2G16700 53.788 132 61 0 40 171 1 132 8.44e-52 162
MS.gene87667.t1 AT3G45990 51.449 138 56 3 40 171 1 133 3.09e-43 140
MS.gene87667.t1 AT2G16700 49.020 102 52 0 36 137 8 109 1.58e-32 112

Find 0 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATCAATCATAAAAAA+TGG - 50850:10058-10077 None:intergenic 10.0%
!! GTATATCATTTACAAGATTA+AGG + 50850:9814-9833 MS.gene87667:CDS 20.0%
! AAGCAACAAAATGATATACT+CGG - 50850:10082-10101 None:intergenic 25.0%
! CAACAGTTAACTTATAGATT+TGG + 50850:10027-10046 MS.gene87667:intron 25.0%
!! TGAATGCAAGTTAAAGTTTT+TGG + 50850:9766-9785 MS.gene87667:CDS 25.0%
!! TGATGTTTGTGAAATTTTAC+AGG + 50850:9712-9731 MS.gene87667:CDS 25.0%
!!! ATTTTGTTGCTTTTGTTTGA+AGG + 50850:10090-10109 MS.gene87667:intron 25.0%
AGCAACAAAATGATATACTC+GGG - 50850:10081-10100 None:intergenic 30.0%
CTTGTAAATGATATACCGAT+AGG - 50850:9810-9829 None:intergenic 30.0%
!! TGAAGAAAATTCTGCTTTTC+TGG - 50850:9973-9992 None:intergenic 30.0%
!!! TGATTTTGATTTTGTCACTG+AGG + 50850:9940-9959 MS.gene87667:CDS 30.0%
AAATCATCGTAACCATTTGC+AGG - 50850:9878-9897 None:intergenic 35.0%
ACATCAGATGCATCAACAAA+AGG - 50850:9698-9717 None:intergenic 35.0%
CTCCAAAGATAGGTTCAAAA+GGG + 50850:10157-10176 MS.gene87667:CDS 35.0%
GTTGTTAGTATGCTTGTTTG+TGG + 50850:9646-9665 MS.gene87667:CDS 35.0%
TCCTTACCCTTGAAATATCA+GGG - 50850:10118-10137 None:intergenic 35.0%
TTTATGCTAGCTCCAAAGAT+AGG + 50850:10147-10166 MS.gene87667:CDS 35.0%
! GCAGAATTTTCTTCATAGCT+TGG + 50850:9978-9997 MS.gene87667:CDS 35.0%
!!! GCTTGGTAATGTCAACTTTT+TGG + 50850:9995-10014 MS.gene87667:intron 35.0%
!!! GTTAAAGTTTTTGGAGCTCA+AGG + 50850:9775-9794 MS.gene87667:CDS 35.0%
ACATCAAGATCCATCTCAGT+AGG - 50850:10221-10240 None:intergenic 40.0%
CTCCTTACCCTTGAAATATC+AGG - 50850:10119-10138 None:intergenic 40.0%
GCTCCAAAGATAGGTTCAAA+AGG + 50850:10156-10175 MS.gene87667:CDS 40.0%
! AAGGTGCCCTGATATTTCAA+GGG + 50850:10109-10128 MS.gene87667:intron 40.0%
! CAAGTTGTTGTTGACAAGGT+TGG + 50850:9848-9867 MS.gene87667:CDS 40.0%
! CTCCCTTTTGAACCTATCTT+TGG - 50850:10162-10181 None:intergenic 40.0%
! GAAGCAAGTTGTTGTTGACA+AGG + 50850:9844-9863 MS.gene87667:CDS 40.0%
AACAGCATATCGACACTCGT+CGG - 50850:9918-9937 None:intergenic 45.0%
AGCTACTGATCCTACTGAGA+TGG + 50850:10208-10227 MS.gene87667:CDS 45.0%
AGGTTCAAAAGGGAGCTTGA+TGG + 50850:10167-10186 MS.gene87667:CDS 45.0%
GCCCTGATATTTCAAGGGTA+AGG + 50850:10114-10133 MS.gene87667:CDS 45.0%
GGTTCAAAAGGGAGCTTGAT+GGG + 50850:10168-10187 MS.gene87667:CDS 45.0%
! TGTGGCGTTGAAAGTGTGTA+TGG + 50850:9664-9683 MS.gene87667:CDS 45.0%
!! AAGGTTGGTGATCCTGCAAA+TGG + 50850:9863-9882 MS.gene87667:CDS 45.0%
!! GAAGGTGCCCTGATATTTCA+AGG + 50850:10108-10127 MS.gene87667:intron 45.0%
TCAAGGCGAAGAGGACCTAT+CGG + 50850:9792-9811 MS.gene87667:CDS 50.0%
TTACAGGCGAATGCAGCATC+AGG + 50850:9728-9747 MS.gene87667:CDS 50.0%
!!! TTTTGGAGCTCAAGGCGAAG+AGG + 50850:9783-9802 MS.gene87667:CDS 50.0%
GGCGAATGCAGCATCAGGTA+TGG + 50850:9733-9752 MS.gene87667:CDS 55.0%
GCATATCGACACTCGTCGGC+AGG - 50850:9914-9933 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
50850 gene 9635 10262 9635 ID=MS.gene87667
50850 mRNA 9635 10262 9635 ID=MS.gene87667.t1;Parent=MS.gene87667
50850 exon 9635 9999 9635 ID=MS.gene87667.t1.exon1;Parent=MS.gene87667.t1
50850 CDS 9635 9999 9635 ID=cds.MS.gene87667.t1;Parent=MS.gene87667.t1
50850 exon 10112 10262 10112 ID=MS.gene87667.t1.exon2;Parent=MS.gene87667.t1
50850 CDS 10112 10262 10112 ID=cds.MS.gene87667.t1;Parent=MS.gene87667.t1
Gene Sequence

>MS.gene87667

ATGGTAATGGGGTTGTTAGTATGCTTGTTTGTGGCGTTGAAAGTGTGTATGGAGAAATTTCCTTTTGTTGATGCATCTGATGTTTGTGAAATTTTACAGGCGAATGCAGCATCAGGTATGGATGTGAATGATGAATGCAAGTTAAAGTTTTTGGAGCTCAAGGCGAAGAGGACCTATCGGTATATCATTTACAAGATTAAGGTGAAGCAGAAGCAAGTTGTTGTTGACAAGGTTGGTGATCCTGCAAATGGTTACGATGATTTCACTGCTAATCTTCCTGCCGACGAGTGTCGATATGCTGTTTATGATTTTGATTTTGTCACTGAGGAGAATTGCCAGAAAAGCAGAATTTTCTTCATAGCTTGGTAATGTCAACTTTTTGGATCTTAAAGCAACAGTTAACTTATAGATTTGGATATTCCATTTTTTATGATTGATTTTAACCCGAGTATATCATTTTGTTGCTTTTGTTTGAAGGTGCCCTGATATTTCAAGGGTAAGGAGCAAGATGATTTATGCTAGCTCCAAAGATAGGTTCAAAAGGGAGCTTGATGGGATTCAAGTTGAGTTGCAAGCTACTGATCCTACTGAGATGGATCTTGATGTGTTCAAAAGCCGTGTCAATTAA

Protein sequence

>MS.gene87667.t1

MVMGLLVCLFVALKVCMEKFPFVDASDVCEILQANAASGMDVNDECKLKFLELKAKRTYRYIIYKIKVKQKQVVVDKVGDPANGYDDFTANLPADECRYAVYDFDFVTEENCQKSRIFFIAWCPDISRVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFKSRVN