AlfalfaGEDB | MS.gene24057

M. sativa cultivar XinJiangDaYe / MS.gene24057

KEGG: K05765
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005856 GO:0007015 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0015629 GO:0016043 GO:0022411 GO:0030042 GO:0032984 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0051261 GO:0071840 GO:0097435 GO:0110165
PFAM: PF00241.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24057.t1	XP_013468343.1	98.6	138	2	0	18	155	2	139	1.20E-70	275.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24057.t1	Q9ZSK3	82.6	138	24	0	18	155	2	139	6.2e-63	241.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24057.t1	G7ZZK8	98.6	138	2	0	18	155	2	139	8.9e-71	275.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058991	MS.gene24057	0.927295	1.43E-91	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene24057	MS.gene058991	PPI
MS.gene41437	MS.gene24057	PPI
MS.gene24057	MS.gene063026	PPI
MS.gene24057	MS.gene037986	PPI
MS.gene24057	MS.gene97005	PPI
MS.gene24057	MS.gene020366	PPI
MS.gene037983	MS.gene24057	PPI
MS.gene24057	MS.gene47853	PPI
MS.gene24057	MS.gene34500	PPI
MS.gene47853	MS.gene24057	PPI
MS.gene037980	MS.gene24057	PPI
MS.gene24057	MS.gene23488	PPI
MS.gene24057	MS.gene037982	PPI
MS.gene87667	MS.gene24057	PPI
MS.gene24057	MS.gene27898	PPI
MS.gene003535	MS.gene24057	PPI
MS.gene02797	MS.gene24057	PPI
MS.gene057694	MS.gene24057	PPI
MS.gene003218	MS.gene24057	PPI
MS.gene018706	MS.gene24057	PPI
MS.gene023659	MS.gene24057	PPI
MS.gene24057	MS.gene037990	PPI
MS.gene037990	MS.gene24057	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24057.t1	MTR_1g068950	98.551	138	2	0	18	155	2	139	5.57e-98	278
MS.gene24057.t1	MTR_2g028670	79.710	138	28	0	18	155	2	139	8.23e-82	238
MS.gene24057.t1	MTR_8g088210	76.812	138	32	0	18	155	2	139	4.41e-78	228
MS.gene24057.t1	MTR_5g010430	74.820	139	33	1	15	153	1	137	5.25e-76	223
MS.gene24057.t1	MTR_8g088210	75.758	132	32	0	24	155	1	132	2.41e-73	216
MS.gene24057.t1	MTR_8g098470	72.794	136	35	1	18	153	2	135	4.70e-73	215
MS.gene24057.t1	MTR_1g101190	72.662	139	36	1	16	154	461	597	2.61e-67	214
MS.gene24057.t1	MTR_7g096890	58.865	141	58	0	15	155	6	146	2.19e-64	194
MS.gene24057.t1	MTR_4g073590	60.448	134	53	0	20	153	8	141	3.05e-58	178
MS.gene24057.t1	MTR_1g076170	60.870	138	54	0	18	155	10	147	4.20e-57	175
MS.gene24057.t1	MTR_4g073590	60.920	87	34	0	20	106	8	94	2.04e-36	121

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24057.t1	AT5G59890	82.609	138	24	0	18	155	2	139	5.87e-85	245
MS.gene24057.t1	AT3G46010	82.609	138	24	0	18	155	13	150	3.65e-84	244
MS.gene24057.t1	AT3G46010	82.609	138	24	0	18	155	2	139	7.15e-84	243
MS.gene24057.t1	AT5G59890	81.818	132	24	0	24	155	1	132	3.91e-80	233
MS.gene24057.t1	AT5G59890	81.818	132	24	0	24	155	1	132	3.91e-80	233
MS.gene24057.t1	AT5G59880	75.362	138	34	0	18	155	2	139	3.92e-78	228
MS.gene24057.t1	AT1G01750	72.222	144	40	0	12	155	1	144	8.27e-78	228
MS.gene24057.t1	AT4G00680	72.222	144	39	1	12	155	7	149	1.91e-77	227
MS.gene24057.t1	AT4G00680	73.188	138	37	0	18	155	2	139	1.31e-76	224
MS.gene24057.t1	AT1G01750	73.913	138	36	0	18	155	2	139	5.50e-76	223
MS.gene24057.t1	AT3G46000	76.812	138	30	1	18	155	2	137	5.74e-76	223
MS.gene24057.t1	AT3G46000	76.812	138	30	1	18	155	21	156	1.13e-75	223
MS.gene24057.t1	AT4G25590	75.735	136	31	1	18	153	2	135	1.87e-73	216
MS.gene24057.t1	AT3G46000	75.758	132	30	1	24	155	1	130	3.35e-71	210
MS.gene24057.t1	AT5G52360	75.000	136	32	1	18	153	2	135	6.25e-71	210
MS.gene24057.t1	AT4G25590	74.615	130	31	1	24	153	1	128	1.08e-68	204
MS.gene24057.t1	AT5G59880	64.493	138	34	1	18	155	2	124	6.07e-63	189
MS.gene24057.t1	AT2G31200	57.447	141	60	0	15	155	6	146	7.41e-61	185
MS.gene24057.t1	AT4G34970	54.745	137	62	0	19	155	5	141	5.69e-55	170
MS.gene24057.t1	AT2G16700	55.224	134	60	0	20	153	8	141	1.91e-54	168
MS.gene24057.t1	AT2G16700	55.385	130	58	0	24	153	1	130	3.01e-52	162
MS.gene24057.t1	AT2G16700	55.385	130	58	0	24	153	1	130	3.01e-52	162
MS.gene24057.t1	AT3G45990	50.725	138	57	3	24	155	1	133	6.30e-43	139
MS.gene24057.t1	AT2G16700	50.000	102	51	0	20	121	8	109	7.41e-34	115

Find 25 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTACCCTTGAAATATC+AGG	0.195342	1.4:-55060681	None:intergenic
TTTATGCTAGCTCCAAAGAT+AGG	0.430442	1.4:+55060712	MS.gene24057:CDS
AAATCATCATAACCATTTGC+AGG	0.445223	1.4:-55060440	None:intergenic
GAAGGTGCCCTGATATTTCA+AGG	0.462729	1.4:+55060673	MS.gene24057:intron
TTACAGGCGAATGCAGCATC+AGG	0.463408	1.4:+55060293	MS.gene24057:CDS
GCTCCAAAGATAGGTTCAAA+AGG	0.469123	1.4:+55060721	MS.gene24057:CDS
GCATATCGACACTCGTCGGC+AGG	0.482779	1.4:-55060476	None:intergenic
GGTTCAAAAGGGAGCTTGAT+GGG	0.491075	1.4:+55060733	MS.gene24057:CDS
ACATCAGATGCATCAACAAA+AGG	0.502015	1.4:-55060260	None:intergenic
GCCCTGATATTTCAAGGGTA+AGG	0.520257	1.4:+55060679	MS.gene24057:CDS
GGCGAATGCAGCATCAGGTA+TGG	0.530905	1.4:+55060298	MS.gene24057:CDS
CAAGTTGTTGTTGACAAGGT+TGG	0.532386	1.4:+55060413	MS.gene24057:CDS
CTCCAAAGATAGGTTCAAAA+GGG	0.546134	1.4:+55060722	MS.gene24057:CDS
AGCTACTGATCCTACTGAGA+TGG	0.549628	1.4:+55060773	MS.gene24057:CDS
AGGTTCAAAAGGGAGCTTGA+TGG	0.551429	1.4:+55060732	MS.gene24057:CDS
TCCTTACCCTTGAAATATCA+GGG	0.558692	1.4:-55060680	None:intergenic
AAGGTTGGTGATCCTGCAAA+TGG	0.580318	1.4:+55060428	MS.gene24057:CDS
GAAGCAAGTTGTTGTTGACA+AGG	0.580658	1.4:+55060409	MS.gene24057:CDS
AAGGTGCCCTGATATTTCAA+GGG	0.592018	1.4:+55060674	MS.gene24057:intron
CTTGTAAATGATATACCGAT+AGG	0.592223	1.4:-55060372	None:intergenic
ACATCAGGATCCATCTCAGT+AGG	0.600240	1.4:-55060783	None:intergenic
AACAGCATATCGACACTCGT+CGG	0.602599	1.4:-55060480	None:intergenic
TCAAGGCGAAGAGGACCTAT+CGG	0.606898	1.4:+55060357	MS.gene24057:CDS
GTATATCATTTACAAGATCG+AGG	0.668947	1.4:+55060379	MS.gene24057:CDS
TCACAAAAGATTAATTGACA+CGG	0.694452	1.4:-55060815	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAATCAATCATAAAAAA+TGG	-	chr1.4:55060623-55060642	None:intergenic	10.0%
!	AAGCAACAAAATGATATACT+CGG	-	chr1.4:55060647-55060666	None:intergenic	25.0%
!	CAACAGTTAACTTATAGATT+TGG	+	chr1.4:55060592-55060611	MS.gene24057:intron	25.0%
!!	TGATGTTTGTGAAATTTTAC+AGG	+	chr1.4:55060277-55060296	MS.gene24057:CDS	25.0%
!!!	ATTTTGTTGCTTTTGTTTGA+AGG	+	chr1.4:55060655-55060674	MS.gene24057:intron	25.0%
	AAATCATCATAACCATTTGC+AGG	-	chr1.4:55060443-55060462	None:intergenic	30.0%
	AGCAACAAAATGATATACTC+GGG	-	chr1.4:55060646-55060665	None:intergenic	30.0%
	CTTGTAAATGATATACCGAT+AGG	-	chr1.4:55060375-55060394	None:intergenic	30.0%
	GTATATCATTTACAAGATCG+AGG	+	chr1.4:55060379-55060398	MS.gene24057:CDS	30.0%
!!	TGAAGAAAATTCTGCTTTTC+TGG	-	chr1.4:55060538-55060557	None:intergenic	30.0%
!!	TGAATGCAAGTTGAAGTTTT+TGG	+	chr1.4:55060331-55060350	MS.gene24057:CDS	30.0%
	ACATCAGATGCATCAACAAA+AGG	-	chr1.4:55060263-55060282	None:intergenic	35.0%
	CTCCAAAGATAGGTTCAAAA+GGG	+	chr1.4:55060722-55060741	MS.gene24057:CDS	35.0%
	TCCTTACCCTTGAAATATCA+GGG	-	chr1.4:55060683-55060702	None:intergenic	35.0%
	TTTATGCTAGCTCCAAAGAT+AGG	+	chr1.4:55060712-55060731	MS.gene24057:CDS	35.0%
!	GCAGAATTTTCTTCATAGCT+TGG	+	chr1.4:55060543-55060562	MS.gene24057:CDS	35.0%
!!!	GCTTGGTAATGTCAACTTTT+TGG	+	chr1.4:55060560-55060579	MS.gene24057:intron	35.0%
!!!	TGATTTTGATTGTGTCACTG+AGG	+	chr1.4:55060505-55060524	MS.gene24057:CDS	35.0%
	CTCCTTACCCTTGAAATATC+AGG	-	chr1.4:55060684-55060703	None:intergenic	40.0%
	GCTCCAAAGATAGGTTCAAA+AGG	+	chr1.4:55060721-55060740	MS.gene24057:CDS	40.0%
!	AAGGTGCCCTGATATTTCAA+GGG	+	chr1.4:55060674-55060693	MS.gene24057:intron	40.0%
!	CAAGTTGTTGTTGACAAGGT+TGG	+	chr1.4:55060413-55060432	MS.gene24057:CDS	40.0%
!	CTCCCTTTTGAACCTATCTT+TGG	-	chr1.4:55060727-55060746	None:intergenic	40.0%
!	GAAGCAAGTTGTTGTTGACA+AGG	+	chr1.4:55060409-55060428	MS.gene24057:CDS	40.0%
!!!	GTTGAAGTTTTTGGAGCTCA+AGG	+	chr1.4:55060340-55060359	MS.gene24057:CDS	40.0%
	AACAGCATATCGACACTCGT+CGG	-	chr1.4:55060483-55060502	None:intergenic	45.0%
	ACATCAGGATCCATCTCAGT+AGG	-	chr1.4:55060786-55060805	None:intergenic	45.0%
	AGCTACTGATCCTACTGAGA+TGG	+	chr1.4:55060773-55060792	MS.gene24057:CDS	45.0%
	AGGTTCAAAAGGGAGCTTGA+TGG	+	chr1.4:55060732-55060751	MS.gene24057:CDS	45.0%
	GCCCTGATATTTCAAGGGTA+AGG	+	chr1.4:55060679-55060698	MS.gene24057:CDS	45.0%
	GGTTCAAAAGGGAGCTTGAT+GGG	+	chr1.4:55060733-55060752	MS.gene24057:CDS	45.0%
!	ACACGGCTTTTGAACACATC+AGG	-	chr1.4:55060801-55060820	None:intergenic	45.0%
!!	AAGGTTGGTGATCCTGCAAA+TGG	+	chr1.4:55060428-55060447	MS.gene24057:CDS	45.0%
!!	GAAGGTGCCCTGATATTTCA+AGG	+	chr1.4:55060673-55060692	MS.gene24057:intron	45.0%
	TCAAGGCGAAGAGGACCTAT+CGG	+	chr1.4:55060357-55060376	MS.gene24057:CDS	50.0%
	TTACAGGCGAATGCAGCATC+AGG	+	chr1.4:55060293-55060312	MS.gene24057:CDS	50.0%
!!!	TTTTGGAGCTCAAGGCGAAG+AGG	+	chr1.4:55060348-55060367	MS.gene24057:CDS	50.0%
	GGCGAATGCAGCATCAGGTA+TGG	+	chr1.4:55060298-55060317	MS.gene24057:CDS	55.0%
	GCATATCGACACTCGTCGGC+AGG	-	chr1.4:55060479-55060498	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	55060248	55060827	55060248	ID=MS.gene24057
chr1.4	mRNA	55060248	55060827	55060248	ID=MS.gene24057.t1;Parent=MS.gene24057
chr1.4	exon	55060248	55060564	55060248	ID=MS.gene24057.t1.exon1;Parent=MS.gene24057.t1
chr1.4	CDS	55060248	55060564	55060248	ID=cds.MS.gene24057.t1;Parent=MS.gene24057.t1
chr1.4	exon	55060677	55060827	55060677	ID=MS.gene24057.t1.exon2;Parent=MS.gene24057.t1
chr1.4	CDS	55060677	55060827	55060677	ID=cds.MS.gene24057.t1;Parent=MS.gene24057.t1

Gene Sequence

>MS.gene24057

ATGGAGAAATTTCCTTTTGTTGATGCATCTGATGTTTGTGAAATTTTACAGGCGAATGCAGCATCAGGTATGGCTGTGAATGATGAATGCAAGTTGAAGTTTTTGGAGCTCAAGGCGAAGAGGACCTATCGGTATATCATTTACAAGATCGAGGAGAAGCAGAAGCAAGTTGTTGTTGACAAGGTTGGTGATCCTGCAAATGGTTATGATGATTTCACTGCTAATCTTCCTGCCGACGAGTGTCGATATGCTGTTTATGATTTTGATTGTGTCACTGAGGAGAATTGCCAGAAAAGCAGAATTTTCTTCATAGCTTGGTAATGTCAACTTTTTGGATCTTAAAGCAACAGTTAACTTATAGATTTGGATATTCCATTTTTTATGATTGATTTTAACCCGAGTATATCATTTTGTTGCTTTTGTTTGAAGGTGCCCTGATATTTCAAGGGTAAGGAGCAAGATGATTTATGCTAGCTCCAAAGATAGGTTCAAAAGGGAGCTTGATGGGATTCAAGTTGAGTTGCAAGCTACTGATCCTACTGAGATGGATCCTGATGTGTTCAAAAGCCGTGTCAATTAA

Protein sequence

>MS.gene24057.t1

MEKFPFVDASDVCEILQANAASGMAVNDECKLKFLELKAKRTYRYIIYKIEEKQKQVVVDKVGDPANGYDDFTANLPADECRYAVYDFDCVTEENCQKSRIFFIAWCPDISRVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDPDVFKSRVN