AlfalfaGEDB | MS.gene037990

M. sativa cultivar XinJiangDaYe / MS.gene037990

KEGG: K05765
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005856 GO:0007015 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0015629 GO:0016043 GO:0022411 GO:0030042 GO:0032984 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0051261 GO:0071840 GO:0097435 GO:0110165
PFAM: PF00241.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037990.t1	XP_013468343.1	97.8	138	3	0	34	171	2	139	2.30E-70	275

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037990.t1	Q9ZSK3	81.9	138	25	0	34	171	2	139	1.2e-62	240.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037990.t1	G7ZZK8	97.8	138	3	0	34	171	2	139	1.7e-70	275.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene037990	MS.gene058991	PPI
MS.gene037990	MS.gene063026	PPI
MS.gene037990	MS.gene037986	PPI
MS.gene037983	MS.gene037990	PPI
MS.gene037990	MS.gene020366	PPI
MS.gene47853	MS.gene037990	PPI
MS.gene037980	MS.gene037990	PPI
MS.gene037990	MS.gene23488	PPI
MS.gene037990	MS.gene34500	PPI
MS.gene87667	MS.gene037990	PPI
MS.gene037990	MS.gene97005	PPI
MS.gene003535	MS.gene037990	PPI
MS.gene02797	MS.gene037990	PPI
MS.gene037990	MS.gene037982	PPI
MS.gene057694	MS.gene037990	PPI
MS.gene003218	MS.gene037990	PPI
MS.gene037990	MS.gene47853	PPI
MS.gene037990	MS.gene27898	PPI
MS.gene018706	MS.gene037990	PPI
MS.gene023659	MS.gene037990	PPI
MS.gene24057	MS.gene037990	PPI
MS.gene41437	MS.gene037990	PPI
MS.gene037990	MS.gene24057	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037990.t1	MTR_1g068950	97.826	138	3	0	34	171	2	139	1.61e-97	278
MS.gene037990.t1	MTR_2g028670	78.261	138	30	0	34	171	2	139	3.20e-81	236
MS.gene037990.t1	MTR_8g088210	76.087	138	33	0	34	171	2	139	7.10e-78	228
MS.gene037990.t1	MTR_5g010430	74.286	140	34	1	31	170	1	138	9.13e-77	225
MS.gene037990.t1	MTR_8g098470	71.533	137	37	1	34	170	2	136	2.54e-73	216
MS.gene037990.t1	MTR_8g088210	75.000	132	33	0	40	171	1	132	5.48e-73	216
MS.gene037990.t1	MTR_1g101190	72.464	138	36	1	32	169	461	596	1.30e-67	216
MS.gene037990.t1	MTR_7g096890	59.574	141	57	0	31	171	6	146	3.98e-65	196
MS.gene037990.t1	MTR_1g076170	61.594	138	53	0	34	171	10	147	4.70e-59	181
MS.gene037990.t1	MTR_4g073590	60.000	135	54	0	36	170	8	142	6.03e-58	178
MS.gene037990.t1	MTR_4g073590	60.920	87	34	0	36	122	8	94	2.19e-35	119

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037990.t1	AT5G59890	81.884	138	25	0	34	171	2	139	2.26e-84	244
MS.gene037990.t1	AT3G46010	81.884	138	25	0	34	171	13	150	8.03e-84	244
MS.gene037990.t1	AT3G46010	81.884	138	25	0	34	171	2	139	1.66e-83	243
MS.gene037990.t1	AT5G59890	81.061	132	25	0	40	171	1	132	1.48e-79	232
MS.gene037990.t1	AT5G59890	81.061	132	25	0	40	171	1	132	1.48e-79	232
MS.gene037990.t1	AT1G01750	72.917	144	39	0	28	171	1	144	7.88e-79	231
MS.gene037990.t1	AT4G00680	72.917	144	38	1	28	171	7	149	1.58e-78	230
MS.gene037990.t1	AT5G59880	74.638	138	35	0	34	171	2	139	9.57e-78	228
MS.gene037990.t1	AT4G00680	73.913	138	36	0	34	171	2	139	1.33e-77	228
MS.gene037990.t1	AT1G01750	74.638	138	35	0	34	171	2	139	4.02e-77	226
MS.gene037990.t1	AT3G46000	76.812	138	30	1	34	171	2	137	2.25e-76	224
MS.gene037990.t1	AT3G46000	76.812	138	30	1	34	171	21	156	4.76e-76	224
MS.gene037990.t1	AT4G25590	75.182	137	32	1	34	170	2	136	6.56e-74	218
MS.gene037990.t1	AT5G52360	75.182	137	32	1	34	170	2	136	7.37e-72	213
MS.gene037990.t1	AT3G46000	75.758	132	30	1	40	171	1	130	1.66e-71	212
MS.gene037990.t1	AT4G25590	74.046	131	32	1	40	170	1	129	5.57e-69	205
MS.gene037990.t1	AT5G59880	63.768	138	35	1	34	171	2	124	1.26e-62	189
MS.gene037990.t1	AT2G31200	58.156	141	59	0	31	171	6	146	1.43e-61	187
MS.gene037990.t1	AT2G16700	53.676	136	63	0	36	171	8	143	5.52e-54	168
MS.gene037990.t1	AT4G34970	53.285	137	64	0	35	171	5	141	5.78e-54	168
MS.gene037990.t1	AT2G16700	53.788	132	61	0	40	171	1	132	8.44e-52	162
MS.gene037990.t1	AT2G16700	53.788	132	61	0	40	171	1	132	8.44e-52	162
MS.gene037990.t1	AT3G45990	51.449	138	56	3	40	171	1	133	3.09e-43	140
MS.gene037990.t1	AT2G16700	49.020	102	52	0	36	137	8	109	1.58e-32	112

Find 30 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTACCCTTGAAATATC+AGG	0.195342	1.1:-50374785	None:intergenic
GTATATCATTTACAAGATTA+AGG	0.294112	1.1:+50374483	MS.gene037990:CDS
TACACTAATCTAATGGTAAT+GGG	0.308098	1.1:+50374292	None:intergenic
CTACACTAATCTAATGGTAA+TGG	0.419474	1.1:+50374291	None:intergenic
TTTATGCTAGCTCCAAAGAT+AGG	0.430442	1.1:+50374816	MS.gene037990:CDS
AAATCATCGTAACCATTTGC+AGG	0.435601	1.1:-50374544	None:intergenic
GAAGGTGCCCTGATATTTCA+AGG	0.462729	1.1:+50374777	MS.gene037990:intron
TTACAGGCGAATGCAGCATC+AGG	0.463408	1.1:+50374397	MS.gene037990:CDS
GCTCCAAAGATAGGTTCAAA+AGG	0.469123	1.1:+50374825	MS.gene037990:CDS
GCATATCGACACTCGTCGGC+AGG	0.482779	1.1:-50374580	None:intergenic
TGTGGCGTTGAAAGTGTGTA+TGG	0.483413	1.1:+50374333	MS.gene037990:CDS
GGTTCAAAAGGGAGCTTGAT+GGG	0.491075	1.1:+50374837	MS.gene037990:CDS
ACATCAGATGCATCAACAAA+AGG	0.502015	1.1:-50374364	None:intergenic
GGCGAATGCAGCATCAGGTA+TGG	0.502868	1.1:+50374402	MS.gene037990:CDS
GCCCTGATATTTCAAGGGTA+AGG	0.520257	1.1:+50374783	MS.gene037990:CDS
CAAGTTGTTGTTGACAAGGT+TGG	0.532386	1.1:+50374517	MS.gene037990:CDS
CTCCAAAGATAGGTTCAAAA+GGG	0.546134	1.1:+50374826	MS.gene037990:CDS
AGCTACTGATCCTACTGAGA+TGG	0.549628	1.1:+50374877	MS.gene037990:CDS
AGGTTCAAAAGGGAGCTTGA+TGG	0.551429	1.1:+50374836	MS.gene037990:CDS
GTTGTTAGTATGCTTGTTTG+TGG	0.553370	1.1:+50374315	MS.gene037990:CDS
TCCTTACCCTTGAAATATCA+GGG	0.558692	1.1:-50374784	None:intergenic
AAGGTTGGTGATCCTGCAAA+TGG	0.580318	1.1:+50374532	MS.gene037990:CDS
GAAGCAAGTTGTTGTTGACA+AGG	0.580658	1.1:+50374513	MS.gene037990:CDS
AAGGTGCCCTGATATTTCAA+GGG	0.592018	1.1:+50374778	MS.gene037990:intron
CTTGTAAATGATATACCGAT+AGG	0.592223	1.1:-50374476	None:intergenic
ACATCAAGATCCATCTCAGT+AGG	0.597944	1.1:-50374887	None:intergenic
AACAGCATATCGACACTCGT+CGG	0.602599	1.1:-50374584	None:intergenic
TCAAGGCGAAGAGGACCTAT+CGG	0.606898	1.1:+50374461	MS.gene037990:CDS
ACACTAATCTAATGGTAATG+GGG	0.642233	1.1:+50374293	None:intergenic
TCACAAAAGATTAATTGACA+CGG	0.694452	1.1:-50374919	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAATCAATCATAAAAAA+TGG	-	chr1.1:50374727-50374746	None:intergenic	10.0%
!!	GTATATCATTTACAAGATTA+AGG	+	chr1.1:50374483-50374502	MS.gene037990:CDS	20.0%
!	AAGCAACAAAATGATATACT+CGG	-	chr1.1:50374751-50374770	None:intergenic	25.0%
!	CAACAGTTAACTTATAGATT+TGG	+	chr1.1:50374696-50374715	MS.gene037990:intron	25.0%
!!	TGAATGCAAGTTAAAGTTTT+TGG	+	chr1.1:50374435-50374454	MS.gene037990:CDS	25.0%
!!	TGATGTTTGTGAAATTTTAC+AGG	+	chr1.1:50374381-50374400	MS.gene037990:CDS	25.0%
!!!	ATTTTGTTGCTTTTGTTTGA+AGG	+	chr1.1:50374759-50374778	MS.gene037990:intron	25.0%
	AGCAACAAAATGATATACTC+GGG	-	chr1.1:50374750-50374769	None:intergenic	30.0%
	CTTGTAAATGATATACCGAT+AGG	-	chr1.1:50374479-50374498	None:intergenic	30.0%
!!	TGAAGAAAATTCTGCTTTTC+TGG	-	chr1.1:50374642-50374661	None:intergenic	30.0%
!!!	TGATTTTGATTTTGTCACTG+AGG	+	chr1.1:50374609-50374628	MS.gene037990:CDS	30.0%
	AAATCATCGTAACCATTTGC+AGG	-	chr1.1:50374547-50374566	None:intergenic	35.0%
	ACATCAGATGCATCAACAAA+AGG	-	chr1.1:50374367-50374386	None:intergenic	35.0%
	CTCCAAAGATAGGTTCAAAA+GGG	+	chr1.1:50374826-50374845	MS.gene037990:CDS	35.0%
	GTTGTTAGTATGCTTGTTTG+TGG	+	chr1.1:50374315-50374334	MS.gene037990:CDS	35.0%
	TCCTTACCCTTGAAATATCA+GGG	-	chr1.1:50374787-50374806	None:intergenic	35.0%
	TTTATGCTAGCTCCAAAGAT+AGG	+	chr1.1:50374816-50374835	MS.gene037990:CDS	35.0%
!	GCAGAATTTTCTTCATAGCT+TGG	+	chr1.1:50374647-50374666	MS.gene037990:CDS	35.0%
!!!	GCTTGGTAATGTCAACTTTT+TGG	+	chr1.1:50374664-50374683	MS.gene037990:intron	35.0%
!!!	GTTAAAGTTTTTGGAGCTCA+AGG	+	chr1.1:50374444-50374463	MS.gene037990:CDS	35.0%
	ACATCAAGATCCATCTCAGT+AGG	-	chr1.1:50374890-50374909	None:intergenic	40.0%
	CTCCTTACCCTTGAAATATC+AGG	-	chr1.1:50374788-50374807	None:intergenic	40.0%
	GCTCCAAAGATAGGTTCAAA+AGG	+	chr1.1:50374825-50374844	MS.gene037990:CDS	40.0%
!	AAGGTGCCCTGATATTTCAA+GGG	+	chr1.1:50374778-50374797	MS.gene037990:intron	40.0%
!	CAAGTTGTTGTTGACAAGGT+TGG	+	chr1.1:50374517-50374536	MS.gene037990:CDS	40.0%
!	CTCCCTTTTGAACCTATCTT+TGG	-	chr1.1:50374831-50374850	None:intergenic	40.0%
!	GAAGCAAGTTGTTGTTGACA+AGG	+	chr1.1:50374513-50374532	MS.gene037990:CDS	40.0%
	AACAGCATATCGACACTCGT+CGG	-	chr1.1:50374587-50374606	None:intergenic	45.0%
	AGCTACTGATCCTACTGAGA+TGG	+	chr1.1:50374877-50374896	MS.gene037990:CDS	45.0%
	AGGTTCAAAAGGGAGCTTGA+TGG	+	chr1.1:50374836-50374855	MS.gene037990:CDS	45.0%
	GCCCTGATATTTCAAGGGTA+AGG	+	chr1.1:50374783-50374802	MS.gene037990:CDS	45.0%
	GGTTCAAAAGGGAGCTTGAT+GGG	+	chr1.1:50374837-50374856	MS.gene037990:CDS	45.0%
!	TGTGGCGTTGAAAGTGTGTA+TGG	+	chr1.1:50374333-50374352	MS.gene037990:CDS	45.0%
!!	AAGGTTGGTGATCCTGCAAA+TGG	+	chr1.1:50374532-50374551	MS.gene037990:CDS	45.0%
!!	GAAGGTGCCCTGATATTTCA+AGG	+	chr1.1:50374777-50374796	MS.gene037990:intron	45.0%
	TCAAGGCGAAGAGGACCTAT+CGG	+	chr1.1:50374461-50374480	MS.gene037990:CDS	50.0%
	TTACAGGCGAATGCAGCATC+AGG	+	chr1.1:50374397-50374416	MS.gene037990:CDS	50.0%
!!!	TTTTGGAGCTCAAGGCGAAG+AGG	+	chr1.1:50374452-50374471	MS.gene037990:CDS	50.0%
	GGCGAATGCAGCATCAGGTA+TGG	+	chr1.1:50374402-50374421	MS.gene037990:CDS	55.0%
	GCATATCGACACTCGTCGGC+AGG	-	chr1.1:50374583-50374602	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	50374304	50374931	50374304	ID=MS.gene037990
chr1.1	mRNA	50374304	50374931	50374304	ID=MS.gene037990.t1;Parent=MS.gene037990
chr1.1	exon	50374304	50374668	50374304	ID=MS.gene037990.t1.exon1;Parent=MS.gene037990.t1
chr1.1	CDS	50374304	50374668	50374304	ID=cds.MS.gene037990.t1;Parent=MS.gene037990.t1
chr1.1	exon	50374781	50374931	50374781	ID=MS.gene037990.t1.exon2;Parent=MS.gene037990.t1
chr1.1	CDS	50374781	50374931	50374781	ID=cds.MS.gene037990.t1;Parent=MS.gene037990.t1

Gene Sequence

>MS.gene037990

ATGGTAATGGGGTTGTTAGTATGCTTGTTTGTGGCGTTGAAAGTGTGTATGGAGAAATTTCCTTTTGTTGATGCATCTGATGTTTGTGAAATTTTACAGGCGAATGCAGCATCAGGTATGGATGTGAATGATGAATGCAAGTTAAAGTTTTTGGAGCTCAAGGCGAAGAGGACCTATCGGTATATCATTTACAAGATTAAGGTGAAGCAGAAGCAAGTTGTTGTTGACAAGGTTGGTGATCCTGCAAATGGTTACGATGATTTCACTGCTAATCTTCCTGCCGACGAGTGTCGATATGCTGTTTATGATTTTGATTTTGTCACTGAGGAGAATTGCCAGAAAAGCAGAATTTTCTTCATAGCTTGGTAATGTCAACTTTTTGGATCTTAAAGCAACAGTTAACTTATAGATTTGGATATTCCATTTTTTATGATTGATTTTAACCCGAGTATATCATTTTGTTGCTTTTGTTTGAAGGTGCCCTGATATTTCAAGGGTAAGGAGCAAGATGATTTATGCTAGCTCCAAAGATAGGTTCAAAAGGGAGCTTGATGGGATTCAAGTTGAGTTGCAAGCTACTGATCCTACTGAGATGGATCTTGATGTGTTCAAAAGCCGTGTCAATTAA

Protein sequence

>MS.gene037990.t1

MVMGLLVCLFVALKVCMEKFPFVDASDVCEILQANAASGMDVNDECKLKFLELKAKRTYRYIIYKIKVKQKQVVVDKVGDPANGYDDFTANLPADECRYAVYDFDFVTEENCQKSRIFFIAWCPDISRVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFKSRVN