AlfalfaGEDB | MS.gene058991

M. sativa cultivar XinJiangDaYe / MS.gene058991

KEGG: K05765
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005856 GO:0007015 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0015629 GO:0016043 GO:0022411 GO:0030042 GO:0032984 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0051261 GO:0071840 GO:0097435 GO:0110165
PFAM: PF00241.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058991.t1	XP_013468343.1	98.6	138	2	0	11	148	2	139	1.50E-70	275.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058991.t1	Q9ZSK3	82.6	138	24	0	11	148	2	139	7.8e-63	241.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058991.t1	G7ZZK8	98.6	138	2	0	11	148	2	139	1.1e-70	275.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058991	MS.gene24057	0.927295	1.43E-91	-1.69E-46
MS.gene058991	MS.gene87667	0.901868	1.73E-78	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene24057	MS.gene058991	PPI
MS.gene058991	MS.gene23488	PPI
MS.gene058991	MS.gene34500	PPI
MS.gene037990	MS.gene058991	PPI
MS.gene057694	MS.gene058991	PPI
MS.gene41437	MS.gene058991	PPI
MS.gene058991	MS.gene97005	PPI
MS.gene018706	MS.gene058991	PPI
MS.gene023659	MS.gene058991	PPI
MS.gene058991	MS.gene037982	PPI
MS.gene058991	MS.gene27898	PPI
MS.gene037983	MS.gene058991	PPI
MS.gene037986	MS.gene058991	PPI
MS.gene87667	MS.gene058991	PPI
MS.gene037982	MS.gene058991	PPI
MS.gene003535	MS.gene058991	PPI
MS.gene47853	MS.gene058991	PPI
MS.gene02797	MS.gene058991	PPI
MS.gene058991	MS.gene037986	PPI
MS.gene037980	MS.gene058991	PPI
MS.gene003218	MS.gene058991	PPI
MS.gene063026	MS.gene058991	PPI
MS.gene058991	MS.gene020366	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058991.t1	MTR_1g068950	98.551	138	2	0	11	148	2	139	1.70e-98	279
MS.gene058991.t1	MTR_2g028670	79.710	138	28	0	11	148	2	139	3.26e-82	238
MS.gene058991.t1	MTR_8g088210	76.812	138	32	0	11	148	2	139	2.01e-78	229
MS.gene058991.t1	MTR_5g010430	75.735	136	31	1	11	146	4	137	3.37e-75	220
MS.gene058991.t1	MTR_8g088210	75.758	132	32	0	17	148	1	132	1.07e-73	216
MS.gene058991.t1	MTR_8g098470	72.794	136	35	1	11	146	2	135	1.84e-73	216
MS.gene058991.t1	MTR_1g101190	73.723	137	34	1	11	147	463	597	1.76e-67	214
MS.gene058991.t1	MTR_7g096890	59.155	142	58	0	7	148	5	146	8.95e-66	197
MS.gene058991.t1	MTR_1g076170	58.904	146	60	0	3	148	2	147	9.29e-60	182
MS.gene058991.t1	MTR_4g073590	60.448	134	53	0	13	146	8	141	2.11e-58	178
MS.gene058991.t1	MTR_4g073590	60.920	87	34	0	13	99	8	94	1.05e-36	122

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058991.t1	AT5G59890	82.609	138	24	0	11	148	2	139	1.53e-85	246
MS.gene058991.t1	AT3G46010	79.310	145	30	0	4	148	6	150	3.10e-85	246
MS.gene058991.t1	AT3G46010	82.609	138	24	0	11	148	2	139	1.97e-84	244
MS.gene058991.t1	AT5G59890	81.818	132	24	0	17	148	1	132	9.65e-81	234
MS.gene058991.t1	AT5G59890	81.818	132	24	0	17	148	1	132	9.65e-81	234
MS.gene058991.t1	AT5G59880	75.362	138	34	0	11	148	2	139	1.89e-78	229
MS.gene058991.t1	AT4G00680	73.188	138	37	0	11	148	2	139	4.65e-77	225
MS.gene058991.t1	AT4G00680	72.340	141	39	0	8	148	9	149	8.52e-77	225
MS.gene058991.t1	AT1G01750	73.913	138	36	0	11	148	2	139	1.95e-76	224
MS.gene058991.t1	AT3G46000	76.812	138	30	1	11	148	2	137	2.35e-76	223
MS.gene058991.t1	AT1G01750	73.913	138	36	0	11	148	7	144	3.28e-76	223
MS.gene058991.t1	AT3G46000	76.812	138	30	1	11	148	21	156	3.96e-76	223
MS.gene058991.t1	AT4G25590	75.735	136	31	1	11	146	2	135	6.60e-74	217
MS.gene058991.t1	AT3G46000	75.758	132	30	1	17	148	1	130	1.17e-71	211
MS.gene058991.t1	AT5G52360	75.000	136	32	1	11	146	2	135	3.53e-71	210
MS.gene058991.t1	AT4G25590	74.615	130	31	1	17	146	1	128	3.89e-69	205
MS.gene058991.t1	AT5G59880	64.493	138	34	1	11	148	2	124	3.40e-63	189
MS.gene058991.t1	AT2G31200	57.746	142	60	0	7	148	5	146	3.46e-62	188
MS.gene058991.t1	AT4G34970	54.745	137	62	0	12	148	5	141	4.65e-55	169
MS.gene058991.t1	AT2G16700	55.224	134	60	0	13	146	8	141	1.39e-54	168
MS.gene058991.t1	AT2G16700	55.385	130	58	0	17	146	1	130	2.14e-52	162
MS.gene058991.t1	AT2G16700	55.385	130	58	0	17	146	1	130	2.14e-52	162
MS.gene058991.t1	AT3G45990	50.725	138	57	3	17	148	1	133	3.75e-43	139
MS.gene058991.t1	AT2G16700	50.000	102	51	0	13	114	8	109	4.72e-34	115

Find 25 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTACCCTTGAAATATC+AGG	0.195342	1.2:-51625897	None:intergenic
TTTATGCTAGCTCCAAAGAT+AGG	0.430442	1.2:+51625928	MS.gene058991:CDS
AAATCATCATAACCATTTGC+AGG	0.445223	1.2:-51625656	None:intergenic
GAAGGTGCCCTGATATTTCA+AGG	0.462729	1.2:+51625889	MS.gene058991:intron
TTACAGGCGAATGCAGCATC+AGG	0.463408	1.2:+51625509	MS.gene058991:intron
GCTCCAAAGATAGGTTCAAA+AGG	0.469123	1.2:+51625937	MS.gene058991:CDS
GAAATGGATTCTCTGCAGTT+AGG	0.472144	1.2:+51624991	None:intergenic
GCATATCGACACTCGTCGGC+AGG	0.482779	1.2:-51625692	None:intergenic
GGTTCAAAAGGGAGCTTGAT+GGG	0.491075	1.2:+51625949	MS.gene058991:CDS
GCCCTGATATTTCAAGGGTA+AGG	0.520257	1.2:+51625895	MS.gene058991:CDS
GGCGAATGCAGCATCAGGTA+TGG	0.530905	1.2:+51625514	MS.gene058991:intron
CAAGTTGTTGTTGACAAGGT+TGG	0.532386	1.2:+51625629	MS.gene058991:CDS
CTCCAAAGATAGGTTCAAAA+GGG	0.546134	1.2:+51625938	MS.gene058991:CDS
AGCTACTGATCCTACTGAGA+TGG	0.549628	1.2:+51625989	MS.gene058991:CDS
AGGTTCAAAAGGGAGCTTGA+TGG	0.551429	1.2:+51625948	MS.gene058991:CDS
TCCTTACCCTTGAAATATCA+GGG	0.558692	1.2:-51625896	None:intergenic
AAGGTTGGTGATCCTGCAAA+TGG	0.580318	1.2:+51625644	MS.gene058991:CDS
GAAGCAAGTTGTTGTTGACA+AGG	0.580658	1.2:+51625625	MS.gene058991:CDS
AAGGTGCCCTGATATTTCAA+GGG	0.592018	1.2:+51625890	MS.gene058991:intron
CTTGTAAATGATATACCGAT+AGG	0.592223	1.2:-51625588	None:intergenic
ACATCAGGATCCATCTCAGT+AGG	0.600240	1.2:-51625999	None:intergenic
AACAGCATATCGACACTCGT+CGG	0.602599	1.2:-51625696	None:intergenic
TCAAGGCGAAGAGGACCTAT+CGG	0.606898	1.2:+51625573	MS.gene058991:CDS
GTATATCATTTACAAGATCG+AGG	0.668947	1.2:+51625595	MS.gene058991:CDS
TCACAAAAGATTAATTGACA+CGG	0.694452	1.2:-51626031	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAATCAATCATAAAAAA+TGG	-	chr1.2:51625839-51625858	None:intergenic	10.0%
!!	AAATTCAAGTCTTAAATATA+TGG	-	chr1.2:51625364-51625383	None:intergenic	15.0%
!!!	TATTTACTAAGCTTTAAATT+TGG	+	chr1.2:51625240-51625259	MS.gene058991:intron	15.0%
!	AAGCAACAAAATGATATACT+CGG	-	chr1.2:51625863-51625882	None:intergenic	25.0%
!	CAACAGTTAACTTATAGATT+TGG	+	chr1.2:51625808-51625827	MS.gene058991:intron	25.0%
!	CATAAATCGCTGATAAAAAT+TGG	-	chr1.2:51625205-51625224	None:intergenic	25.0%
!	TACACTAATCTAATGGTAAT+GGG	+	chr1.2:51625404-51625423	MS.gene058991:intron	25.0%
!	TTAAAGCTACACTAATCTAA+TGG	+	chr1.2:51625397-51625416	MS.gene058991:intron	25.0%
!!	TGATGTTTGTGAAATTTTAC+AGG	+	chr1.2:51625493-51625512	MS.gene058991:intron	25.0%
!!!	ATTTTGTTGCTTTTGTTTGA+AGG	+	chr1.2:51625871-51625890	MS.gene058991:intron	25.0%
	AAATCATCATAACCATTTGC+AGG	-	chr1.2:51625659-51625678	None:intergenic	30.0%
	AGCAACAAAATGATATACTC+GGG	-	chr1.2:51625862-51625881	None:intergenic	30.0%
	CTACACTAATCTAATGGTAA+TGG	+	chr1.2:51625403-51625422	MS.gene058991:intron	30.0%
	CTTGTAAATGATATACCGAT+AGG	-	chr1.2:51625591-51625610	None:intergenic	30.0%
	GTATATCATTTACAAGATCG+AGG	+	chr1.2:51625595-51625614	MS.gene058991:CDS	30.0%
	TTACATTGTGAATCTTCCAA+TGG	+	chr1.2:51625077-51625096	MS.gene058991:intron	30.0%
!	ACACTAATCTAATGGTAATG+GGG	+	chr1.2:51625405-51625424	MS.gene058991:intron	30.0%
!!	TGAAGAAAATTCTGCTTTTC+TGG	-	chr1.2:51625754-51625773	None:intergenic	30.0%
!!	TGAATGCAAGTTGAAGTTTT+TGG	+	chr1.2:51625547-51625566	MS.gene058991:CDS	30.0%
	ACATCAGATGCATCAACAAA+AGG	-	chr1.2:51625479-51625498	None:intergenic	35.0%
	CAGCGATGATGAAAATTAGA+CGG	+	chr1.2:51625034-51625053	MS.gene058991:intron	35.0%
	CTCCAAAGATAGGTTCAAAA+GGG	+	chr1.2:51625938-51625957	MS.gene058991:CDS	35.0%
	GGCTGTTTGATTGAAATCAT+CGG	-	chr1.2:51625267-51625286	None:intergenic	35.0%
	GTTGTTAGTATGCTTGTTTG+TGG	+	chr1.2:51625427-51625446	MS.gene058991:intron	35.0%
	TCCTTACCCTTGAAATATCA+GGG	-	chr1.2:51625899-51625918	None:intergenic	35.0%
	TTTATGCTAGCTCCAAAGAT+AGG	+	chr1.2:51625928-51625947	MS.gene058991:CDS	35.0%
!	GCAGAATTTTCTTCATTGCT+TGG	+	chr1.2:51625759-51625778	MS.gene058991:CDS	35.0%
!!!	GCTTGGTAATGTCAACTTTT+TGG	+	chr1.2:51625776-51625795	MS.gene058991:intron	35.0%
!!!	TGATTTTGATTGTGTCACTG+AGG	+	chr1.2:51625721-51625740	MS.gene058991:CDS	35.0%
	AACAGTCAAATCCAAGCCAT+TGG	-	chr1.2:51625096-51625115	None:intergenic	40.0%
	ATCCGGGTTTGATTGCAAAA+TGG	+	chr1.2:51625136-51625155	MS.gene058991:intron	40.0%
	CTCCTTACCCTTGAAATATC+AGG	-	chr1.2:51625900-51625919	None:intergenic	40.0%
	GAGCCAACTACACTTATCAA+CGG	-	chr1.2:51625342-51625361	None:intergenic	40.0%
	GCTCCAAAGATAGGTTCAAA+AGG	+	chr1.2:51625937-51625956	MS.gene058991:CDS	40.0%
	TCGGTCAAATAGCAAGTACA+AGG	+	chr1.2:51625165-51625184	MS.gene058991:intron	40.0%
	TGACTGTTGTGATTTCTGAC+CGG	+	chr1.2:51625108-51625127	MS.gene058991:intron	40.0%
	TTGTGAATCTTCCAATGGCT+TGG	+	chr1.2:51625082-51625101	MS.gene058991:intron	40.0%
!	AAGGTGCCCTGATATTTCAA+GGG	+	chr1.2:51625890-51625909	MS.gene058991:intron	40.0%
!	ACATAGGATTTTCTGCCGTT+AGG	+	chr1.2:51625306-51625325	MS.gene058991:intron	40.0%
!	ACTACACTTATCAACGGACT+TGG	-	chr1.2:51625336-51625355	None:intergenic	40.0%
!	AGTCCGTTGATAAGTGTAGT+TGG	+	chr1.2:51625336-51625355	MS.gene058991:intron	40.0%
!	CAAGTTGTTGTTGACAAGGT+TGG	+	chr1.2:51625629-51625648	MS.gene058991:CDS	40.0%
!	CATAGGATTTTCTGCCGTTA+GGG	+	chr1.2:51625307-51625326	MS.gene058991:intron	40.0%
!	CTCCCTTTTGAACCTATCTT+TGG	-	chr1.2:51625943-51625962	None:intergenic	40.0%
!	GAAGCAAGTTGTTGTTGACA+AGG	+	chr1.2:51625625-51625644	MS.gene058991:CDS	40.0%
!!	AGCCATTTTGCAATCAAACC+CGG	-	chr1.2:51625141-51625160	None:intergenic	40.0%
!!	TGTACTTGCTATTTGACCGA+GGG	-	chr1.2:51625165-51625184	None:intergenic	40.0%
!!	TTGTACTTGCTATTTGACCG+AGG	-	chr1.2:51625166-51625185	None:intergenic	40.0%
!!!	GTTGAAGTTTTTGGAGCTCA+AGG	+	chr1.2:51625556-51625575	MS.gene058991:CDS	40.0%
	AACAGCATATCGACACTCGT+CGG	-	chr1.2:51625699-51625718	None:intergenic	45.0%
	AATCAAACCCGGATTACCTC+CGG	-	chr1.2:51625130-51625149	None:intergenic	45.0%
	ACATCAGGATCCATCTCAGT+AGG	-	chr1.2:51626002-51626021	None:intergenic	45.0%
	AGCTACTGATCCTACTGAGA+TGG	+	chr1.2:51625989-51626008	MS.gene058991:CDS	45.0%
	AGGTTCAAAAGGGAGCTTGA+TGG	+	chr1.2:51625948-51625967	MS.gene058991:CDS	45.0%
	ATTTCTGACCGGAGGTAATC+CGG	+	chr1.2:51625119-51625138	MS.gene058991:intron	45.0%
	GATTGCAAAATGGCTTCCCT+CGG	+	chr1.2:51625146-51625165	MS.gene058991:intron	45.0%
	GCCCTGATATTTCAAGGGTA+AGG	+	chr1.2:51625895-51625914	MS.gene058991:CDS	45.0%
	GGTTCAAAAGGGAGCTTGAT+GGG	+	chr1.2:51625949-51625968	MS.gene058991:CDS	45.0%
!	AACGGACTTGGATTCCCTAA+CGG	-	chr1.2:51625324-51625343	None:intergenic	45.0%
!	ACACGGCTTTTGAACACATC+AGG	-	chr1.2:51626017-51626036	None:intergenic	45.0%
!	TGTGGCGTTGAAAGTGTGTA+TGG	+	chr1.2:51625445-51625464	MS.gene058991:intron	45.0%
!!	AAGGTTGGTGATCCTGCAAA+TGG	+	chr1.2:51625644-51625663	MS.gene058991:CDS	45.0%
!!	GAAGGTGCCCTGATATTTCA+AGG	+	chr1.2:51625889-51625908	MS.gene058991:intron	45.0%
	CTGTTGTGATTTCTGACCGG+AGG	+	chr1.2:51625111-51625130	MS.gene058991:intron	50.0%
	GTGCAGTCAGTGCACACTAA+AGG	-	chr1.2:51625288-51625307	None:intergenic	50.0%
	TCAAGGCGAAGAGGACCTAT+CGG	+	chr1.2:51625573-51625592	MS.gene058991:CDS	50.0%
	TTACAGGCGAATGCAGCATC+AGG	+	chr1.2:51625509-51625528	MS.gene058991:intron	50.0%
	TTTCTGACCGGAGGTAATCC+GGG	+	chr1.2:51625120-51625139	MS.gene058991:intron	50.0%
!!	AGTGTGCACTGACTGCACAT+AGG	+	chr1.2:51625290-51625309	MS.gene058991:intron	50.0%
!!!	TTTTGGAGCTCAAGGCGAAG+AGG	+	chr1.2:51625564-51625583	MS.gene058991:CDS	50.0%
	GGCGAATGCAGCATCAGGTA+TGG	+	chr1.2:51625514-51625533	MS.gene058991:intron	55.0%
	GCATATCGACACTCGTCGGC+AGG	-	chr1.2:51625695-51625714	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	51624994	51626043	51624994	ID=MS.gene058991
chr1.2	mRNA	51624994	51626043	51624994	ID=MS.gene058991.t1;Parent=MS.gene058991
chr1.2	exon	51624994	51625023	51624994	ID=MS.gene058991.t1.exon1;Parent=MS.gene058991.t1
chr1.2	CDS	51624994	51625023	51624994	ID=cds.MS.gene058991.t1;Parent=MS.gene058991.t1
chr1.2	exon	51625515	51625780	51625515	ID=MS.gene058991.t1.exon2;Parent=MS.gene058991.t1
chr1.2	CDS	51625515	51625780	51625515	ID=cds.MS.gene058991.t1;Parent=MS.gene058991.t1
chr1.2	exon	51625893	51626043	51625893	ID=MS.gene058991.t1.exon3;Parent=MS.gene058991.t1
chr1.2	CDS	51625893	51626043	51625893	ID=cds.MS.gene058991.t1;Parent=MS.gene058991.t1

Gene Sequence

>MS.gene058991

ATGGATTCTCTGCAGTTAGGTGTCACTGCAGTTACTATCTCAGCGATGATGAAAATTAGACGGTTAAGATTCATTTGTCAAACTTACATTGTGAATCTTCCAATGGCTTGGATTTGACTGTTGTGATTTCTGACCGGAGGTAATCCGGGTTTGATTGCAAAATGGCTTCCCTCGGTCAAATAGCAAGTACAAGGATCAACTCAAGCATCCAATTTTTATCAGCGATTTATGAATTATACTTTTATTTATTTACTAAGCTTTAAATTTGGACCGATGATTTCAATCAAACAGCCTTTAGTGTGCACTGACTGCACATAGGATTTTCTGCCGTTAGGGAATCCAAGTCCGTTGATAAGTGTAGTTGGCTCCATATATTTAAGACTTGAATTTTTGTTGTTGATCGTTAAAGCTACACTAATCTAATGGTAATGGGGTTGTTAGTATGCTTGTTTGTGGCGTTGAAAGTGTGTATGGAGAAATTTCCTTTTGTTGATGCATCTGATGTTTGTGAAATTTTACAGGCGAATGCAGCATCAGGTATGGCTGTGAATGATGAATGCAAGTTGAAGTTTTTGGAGCTCAAGGCGAAGAGGACCTATCGGTATATCATTTACAAGATCGAGGAGAAGCAGAAGCAAGTTGTTGTTGACAAGGTTGGTGATCCTGCAAATGGTTATGATGATTTCACTGCTAATCTTCCTGCCGACGAGTGTCGATATGCTGTTTATGATTTTGATTGTGTCACTGAGGAGAATTGCCAGAAAAGCAGAATTTTCTTCATTGCTTGGTAATGTCAACTTTTTGGATCTTAAAGCAACAGTTAACTTATAGATTTGGATATTCCATTTTTTATGATTGATTTTAACCCGAGTATATCATTTTGTTGCTTTTGTTTGAAGGTGCCCTGATATTTCAAGGGTAAGGAGCAAGATGATTTATGCTAGCTCCAAAGATAGGTTCAAAAGGGAGCTTGATGGGATTCAAGTTGAGTTGCAAGCTACTGATCCTACTGAGATGGATCCTGATGTGTTCAAAAGCCGTGTCAATTAA

Protein sequence

>MS.gene058991.t1

MDSLQLGVTAANAASGMAVNDECKLKFLELKAKRTYRYIIYKIEEKQKQVVVDKVGDPANGYDDFTANLPADECRYAVYDFDCVTEENCQKSRIFFIAWCPDISRVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDPDVFKSRVN