AlfalfaGEDB | MS.gene063026

M. sativa cultivar XinJiangDaYe / MS.gene063026

KEGG: K05765
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005856 GO:0007015 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0015629 GO:0016043 GO:0022411 GO:0030042 GO:0032984 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0051261 GO:0071840 GO:0097435 GO:0110165
PFAM: PF00241.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063026.t1	XP_013456470.1	100	143	0	0	1	143	1	143	8.20E-77	296.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063026.t1	Q9ZNT3	86.0	143	20	0	1	143	1	143	1.5e-66	253.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063026.t1	A0A072UM89	100.0	143	0	0	1	143	1	143	5.9e-77	296.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049129	MS.gene063026	-0.843227	1.60E-58	-1.69E-46
MS.gene049255	MS.gene063026	0.808584	2.88E-50	-1.69E-46
MS.gene049411	MS.gene063026	0.818304	2.10E-52	-1.69E-46
MS.gene049580	MS.gene063026	0.810281	1.25E-50	-1.69E-46
MS.gene050277	MS.gene063026	0.801653	8.14E-49	-1.69E-46
MS.gene050437	MS.gene063026	0.848365	6.42E-60	-1.69E-46
MS.gene050689	MS.gene063026	0.80886	2.52E-50	-1.69E-46
MS.gene050752	MS.gene063026	0.847894	8.66E-60	-1.69E-46
MS.gene050838	MS.gene063026	0.801935	7.12E-49	-1.69E-46
MS.gene050955	MS.gene063026	0.839483	1.54E-57	-1.69E-46
MS.gene051003	MS.gene063026	0.809296	2.03E-50	-1.69E-46
MS.gene051234	MS.gene063026	0.808492	3.02E-50	-1.69E-46
MS.gene051241	MS.gene063026	0.801466	8.89E-49	-1.69E-46
MS.gene051328	MS.gene063026	0.804401	2.20E-49	-1.69E-46
MS.gene051343	MS.gene063026	0.82253	2.26E-53	-1.69E-46
MS.gene05134	MS.gene063026	0.830175	3.41E-55	-1.69E-46
MS.gene051903	MS.gene063026	0.811013	8.66E-51	-1.69E-46
MS.gene052072	MS.gene063026	0.822171	2.73E-53	-1.69E-46
MS.gene052793	MS.gene063026	0.802392	5.73E-49	-1.69E-46
MS.gene052947	MS.gene063026	0.853193	2.81E-61	-1.69E-46
MS.gene053590	MS.gene063026	0.824736	6.87E-54	-1.69E-46
MS.gene05364	MS.gene063026	0.812759	3.61E-51	-1.69E-46
MS.gene054004	MS.gene063026	0.810302	1.23E-50	-1.69E-46
MS.gene054403	MS.gene063026	0.850136	2.06E-60	-1.69E-46
MS.gene05469	MS.gene063026	0.809004	2.34E-50	-1.69E-46
MS.gene055555	MS.gene063026	0.800096	1.69E-48	-1.69E-46
MS.gene057234	MS.gene063026	0.806335	8.65E-50	-1.69E-46
MS.gene057392	MS.gene063026	0.822461	2.34E-53	-1.69E-46
MS.gene057694	MS.gene063026	0.885871	5.59E-72	-1.69E-46
MS.gene057705	MS.gene063026	0.864829	9.17E-65	-1.69E-46
MS.gene058976	MS.gene063026	0.805741	1.15E-49	-1.69E-46
MS.gene059146	MS.gene063026	0.800149	1.65E-48	-1.69E-46
MS.gene060244	MS.gene063026	0.83981	1.27E-57	-1.69E-46
MS.gene060365	MS.gene063026	0.808901	2.47E-50	-1.69E-46
MS.gene060917	MS.gene063026	0.801103	1.05E-48	-1.69E-46
MS.gene061100	MS.gene063026	0.803693	3.09E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene003218	MS.gene063026	PPI
MS.gene063026	MS.gene037986	PPI
MS.gene018706	MS.gene063026	PPI
MS.gene063026	MS.gene34500	PPI
MS.gene24057	MS.gene063026	PPI
MS.gene063026	MS.gene97005	PPI
MS.gene037990	MS.gene063026	PPI
MS.gene41437	MS.gene063026	PPI
MS.gene063026	MS.gene27898	PPI
MS.gene023659	MS.gene063026	PPI
MS.gene063026	MS.gene037982	PPI
MS.gene037983	MS.gene063026	PPI
MS.gene037980	MS.gene063026	PPI
MS.gene87667	MS.gene063026	PPI
MS.gene47853	MS.gene063026	PPI
MS.gene063026	MS.gene020366	PPI
MS.gene02797	MS.gene063026	PPI
MS.gene063026	MS.gene058991	PPI
MS.gene063026	MS.gene23488	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063026.t1	MTR_4g073590	100.000	143	0	0	1	143	1	143	1.06e-105	297
MS.gene063026.t1	MTR_7g096890	68.889	135	42	0	8	142	11	145	6.70e-71	209
MS.gene063026.t1	MTR_4g073590	100.000	94	0	0	1	94	1	94	1.10e-67	200
MS.gene063026.t1	MTR_1g076170	62.329	146	49	1	3	142	1	146	7.95e-62	187
MS.gene063026.t1	MTR_1g068950	61.194	134	52	0	8	141	4	137	2.21e-60	183
MS.gene063026.t1	MTR_5g010430	60.000	135	52	1	8	142	6	138	8.30e-60	181
MS.gene063026.t1	MTR_8g088210	57.778	135	57	0	8	142	4	138	2.44e-57	175
MS.gene063026.t1	MTR_2g028670	55.556	135	60	0	8	142	4	138	1.29e-56	173
MS.gene063026.t1	MTR_8g098470	57.037	135	56	1	8	142	4	136	1.00e-55	171
MS.gene063026.t1	MTR_8g088210	58.015	131	55	0	12	142	1	131	2.47e-55	170
MS.gene063026.t1	MTR_1g101190	59.259	135	53	1	8	142	465	597	7.22e-54	178

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063026.t1	AT2G16700	86.014	143	20	0	1	143	1	143	1.19e-89	257
MS.gene063026.t1	AT4G34970	80.851	141	27	0	3	143	1	141	2.31e-86	248
MS.gene063026.t1	AT2G16700	84.848	132	20	0	12	143	1	132	4.82e-81	235
MS.gene063026.t1	AT2G16700	84.848	132	20	0	12	143	1	132	4.82e-81	235
MS.gene063026.t1	AT2G31200	62.222	135	51	0	8	142	11	145	2.61e-62	188
MS.gene063026.t1	AT4G00680	59.701	134	54	0	8	141	4	137	2.19e-61	185
MS.gene063026.t1	AT4G00680	59.701	134	54	0	8	141	14	147	4.15e-61	185
MS.gene063026.t1	AT1G01750	59.701	134	54	0	8	141	4	137	1.94e-60	183
MS.gene063026.t1	AT1G01750	59.701	134	54	0	8	141	9	142	2.51e-60	183
MS.gene063026.t1	AT5G59890	59.701	134	54	0	8	141	4	137	1.46e-59	181
MS.gene063026.t1	AT3G46010	58.519	135	56	0	8	142	15	149	2.49e-59	181
MS.gene063026.t1	AT3G46010	58.519	135	56	0	8	142	4	138	3.17e-59	180
MS.gene063026.t1	AT4G25590	60.741	135	51	1	8	142	4	136	4.36e-59	179
MS.gene063026.t1	AT5G59880	57.037	135	58	0	8	142	4	138	3.34e-58	177
MS.gene063026.t1	AT3G46000	60.448	134	51	1	8	141	4	135	1.14e-57	176
MS.gene063026.t1	AT5G59890	60.000	130	52	0	12	141	1	130	1.44e-57	176
MS.gene063026.t1	AT5G59890	60.000	130	52	0	12	141	1	130	1.44e-57	176
MS.gene063026.t1	AT3G46000	60.448	134	51	1	8	141	23	154	1.50e-57	176
MS.gene063026.t1	AT4G25590	61.069	131	49	1	12	142	1	129	4.18e-57	174
MS.gene063026.t1	AT2G16700	77.982	109	24	0	1	109	1	109	4.89e-56	171
MS.gene063026.t1	AT5G52360	57.778	135	55	1	8	142	4	136	7.97e-56	171
MS.gene063026.t1	AT3G46000	60.769	130	49	1	12	141	1	128	1.17e-55	171
MS.gene063026.t1	AT5G59880	52.593	135	49	1	8	142	4	123	1.96e-48	152
MS.gene063026.t1	AT3G45990	40.000	135	72	3	12	141	1	131	1.76e-31	109

Find 30 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTAAGAGGGGCATCTATT+TGG	0.279597	4.2:-34975613	None:intergenic
AATGGCGATGGCTTTCAAAA+TGG	0.335809	4.2:+34974562	None:intergenic
GTTGACAAGGTTGGTGGTCC+TGG	0.380021	4.2:+34974930	MS.gene063026:CDS
TCTCTTATTCTTGATGCTGT+TGG	0.381519	4.2:-34975474	None:intergenic
GGATGGGTTAAGAAGAGCTT+TGG	0.389969	4.2:+34975521	MS.gene063026:CDS
ACTCTTTCATGGAGATGAAA+TGG	0.399104	4.2:+34974850	MS.gene063026:CDS
GAATCTGAACAGGCTACTAC+TGG	0.427593	4.2:+34974801	MS.gene063026:intron
TGAGTGCAAGAACTCTTTCA+TGG	0.435498	4.2:+34974839	MS.gene063026:CDS
TCTCAGTTGGATCGGTTGCT+TGG	0.449998	4.2:-34975563	None:intergenic
GCATATCTACAATCATCATT+AGG	0.474554	4.2:-34974984	None:intergenic
AACAGGCTACTACTGGGATG+TGG	0.527418	4.2:+34974808	MS.gene063026:intron
AGCAACCGATCCAACTGAGA+TGG	0.533493	4.2:+34975566	MS.gene063026:CDS
ACATCGAATCCCATCTCAGT+TGG	0.534338	4.2:-34975576	None:intergenic
CATGGAGATGAAATGGAAGA+AGG	0.536633	4.2:+34974857	MS.gene063026:CDS
GCAACCGATCCAACTGAGAT+GGG	0.549755	4.2:+34975567	MS.gene063026:CDS
GCAAGATCTTCTTCATTGCA+TGG	0.552818	4.2:+34975051	MS.gene063026:CDS
TGAAGAAGATCTTGCTCTTG+CGG	0.553047	4.2:-34975043	None:intergenic
GATACTATACGCAACATCAA+AGG	0.562150	4.2:+34975500	MS.gene063026:CDS
GGGTTAAGAAGAGCTTTGGA+TGG	0.582337	4.2:+34975525	MS.gene063026:CDS
TGAAATGGAAGAAGGTGCAT+AGG	0.587717	4.2:+34974865	MS.gene063026:CDS
GTGACCGTTGACAAGGTTGG+TGG	0.590709	4.2:+34974924	MS.gene063026:CDS
GAATCCCATCTCAGTTGGAT+CGG	0.598606	4.2:-34975571	None:intergenic
CTATACGCAACATCAAAGGA+TGG	0.610779	4.2:+34975504	MS.gene063026:CDS
AAGGCTTGTGACCGTTGACA+AGG	0.611864	4.2:+34974917	MS.gene063026:CDS
ACAGGCTACTACTGGGATGT+GGG	0.613046	4.2:+34974809	MS.gene063026:intron
TCATCAGATAACAATGGCGA+TGG	0.623350	4.2:+34974550	None:intergenic
TATACGCAACATCAAAGGAT+GGG	0.626471	4.2:+34975505	MS.gene063026:CDS
AATCTGAACAGGCTACTACT+GGG	0.632062	4.2:+34974802	MS.gene063026:intron
AGGACCACCAACCTTGTCAA+CGG	0.633960	4.2:-34974928	None:intergenic
CTTGTGACCGTTGACAAGGT+TGG	0.653631	4.2:+34974921	MS.gene063026:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAATCAATTTAGTAT+AGG	-	chr4.2:34975257-34975276	None:intergenic	10.0%
!!!	TTTTTTTTTTTACTTTTACT+TGG	+	chr4.2:34975269-34975288	MS.gene063026:intron	10.0%
!!	TTTGTTTATACACATATTTA+TGG	+	chr4.2:34974694-34974713	MS.gene063026:intron	15.0%
!!	TTTAATCCAACAATTGAAAT+TGG	+	chr4.2:34975145-34975164	MS.gene063026:intron	20.0%
!!!	TAATTTTGGGTAAAATTTTC+AGG	+	chr4.2:34975448-34975467	MS.gene063026:intron	20.0%
!	ATCACATCATATGGTATTAT+TGG	-	chr4.2:34975352-34975371	None:intergenic	25.0%
!	CAAATAATATGAATCTGAAC+AGG	+	chr4.2:34974791-34974810	MS.gene063026:intron	25.0%
!	TAAATTGTACTACTAGTACT+TGG	-	chr4.2:34975301-34975320	None:intergenic	25.0%
!	TATGAGTGAGATGATTTAAA+AGG	-	chr4.2:34974598-34974617	None:intergenic	25.0%
!	TCAATTGTTGGATTAAACAA+AGG	-	chr4.2:34975142-34975161	None:intergenic	25.0%
!	TCACATCATATGGTATTATT+GGG	-	chr4.2:34975351-34975370	None:intergenic	25.0%
	CATTGAGCAAACATTGTTAT+TGG	-	chr4.2:34975118-34975137	None:intergenic	30.0%
	GCATATCTACAATCATCATT+AGG	-	chr4.2:34974987-34975006	None:intergenic	30.0%
	TAGAGTCCAATTTCAATTGT+TGG	-	chr4.2:34975154-34975173	None:intergenic	30.0%
	TATATGAGTCTAATACAAGC+TGG	+	chr4.2:34975325-34975344	MS.gene063026:intron	30.0%
	TCAAGATCGATGAAAAAACA+AGG	+	chr4.2:34974898-34974917	MS.gene063026:CDS	30.0%
	TGTACATTCATCACATCATA+TGG	-	chr4.2:34975361-34975380	None:intergenic	30.0%
!	TGAATGTGAGGGATAATTTT+GGG	+	chr4.2:34975435-34975454	MS.gene063026:intron	30.0%
	ACACACAATTAGATCGTCTA+CGG	-	chr4.2:34975190-34975209	None:intergenic	35.0%
	ACGATCTAATTGTGTGTGTT+TGG	+	chr4.2:34975193-34975212	MS.gene063026:intron	35.0%
	ACTCTTTCATGGAGATGAAA+TGG	+	chr4.2:34974850-34974869	MS.gene063026:CDS	35.0%
	GATACTATACGCAACATCAA+AGG	+	chr4.2:34975500-34975519	MS.gene063026:CDS	35.0%
	GATGATGAATGTGAATGTGA+GGG	+	chr4.2:34975424-34975443	MS.gene063026:intron	35.0%
	TATACGCAACATCAAAGGAT+GGG	+	chr4.2:34975505-34975524	MS.gene063026:CDS	35.0%
	TCTCTTATTCTTGATGCTGT+TGG	-	chr4.2:34975477-34975496	None:intergenic	35.0%
!	AGATCATCATAGTTTTCTCC+AGG	-	chr4.2:34974951-34974970	None:intergenic	35.0%
!	GTGAATGTGAGGGATAATTT+TGG	+	chr4.2:34975434-34975453	MS.gene063026:intron	35.0%
!	TGATGATGAATGTGAATGTG+AGG	+	chr4.2:34975423-34975442	MS.gene063026:intron	35.0%
	AATCTGAACAGGCTACTACT+GGG	+	chr4.2:34974802-34974821	MS.gene063026:intron	40.0%
	AATTGGACTCTACAAAGCTG+TGG	+	chr4.2:34975162-34975181	MS.gene063026:intron	40.0%
	CATGGAGATGAAATGGAAGA+AGG	+	chr4.2:34974857-34974876	MS.gene063026:CDS	40.0%
	CTATACGCAACATCAAAGGA+TGG	+	chr4.2:34975504-34975523	MS.gene063026:CDS	40.0%
	GCAAGATCTTCTTCATTGCA+TGG	+	chr4.2:34975051-34975070	MS.gene063026:CDS	40.0%
	TGAAGAAGATCTTGCTCTTG+CGG	-	chr4.2:34975046-34975065	None:intergenic	40.0%
	TGAGTGCAAGAACTCTTTCA+TGG	+	chr4.2:34974839-34974858	MS.gene063026:CDS	40.0%
!	TGAAATGGAAGAAGGTGCAT+AGG	+	chr4.2:34974865-34974884	MS.gene063026:CDS	40.0%
	ACATCGAATCCCATCTCAGT+TGG	-	chr4.2:34975579-34975598	None:intergenic	45.0%
	GAATCCCATCTCAGTTGGAT+CGG	-	chr4.2:34975574-34975593	None:intergenic	45.0%
	GAATCTGAACAGGCTACTAC+TGG	+	chr4.2:34974801-34974820	MS.gene063026:intron	45.0%
!!	GGATGGGTTAAGAAGAGCTT+TGG	+	chr4.2:34975521-34975540	MS.gene063026:CDS	45.0%
!!	GGGTTAAGAAGAGCTTTGGA+TGG	+	chr4.2:34975525-34975544	MS.gene063026:CDS	45.0%
	AACAGGCTACTACTGGGATG+TGG	+	chr4.2:34974808-34974827	MS.gene063026:intron	50.0%
	AAGGCTTGTGACCGTTGACA+AGG	+	chr4.2:34974917-34974936	MS.gene063026:CDS	50.0%
	ACAGGCTACTACTGGGATGT+GGG	+	chr4.2:34974809-34974828	MS.gene063026:intron	50.0%
	AGCAACCGATCCAACTGAGA+TGG	+	chr4.2:34975566-34975585	MS.gene063026:CDS	50.0%
	AGGACCACCAACCTTGTCAA+CGG	-	chr4.2:34974931-34974950	None:intergenic	50.0%
	CTTGTGACCGTTGACAAGGT+TGG	+	chr4.2:34974921-34974940	MS.gene063026:CDS	50.0%
	GCAACCGATCCAACTGAGAT+GGG	+	chr4.2:34975567-34975586	MS.gene063026:CDS	50.0%
	TCTCAGTTGGATCGGTTGCT+TGG	-	chr4.2:34975566-34975585	None:intergenic	50.0%
	GTGACCGTTGACAAGGTTGG+TGG	+	chr4.2:34974924-34974943	MS.gene063026:CDS	55.0%
!!	GTTGACAAGGTTGGTGGTCC+TGG	+	chr4.2:34974930-34974949	MS.gene063026:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	34974563	34975620	34974563	ID=MS.gene063026
chr4.2	mRNA	34974563	34975620	34974563	ID=MS.gene063026.t1;Parent=MS.gene063026
chr4.2	exon	34974563	34974583	34974563	ID=MS.gene063026.t1.exon1;Parent=MS.gene063026.t1
chr4.2	CDS	34974563	34974583	34974563	ID=cds.MS.gene063026.t1;Parent=MS.gene063026.t1
chr4.2	exon	34974813	34975072	34974813	ID=MS.gene063026.t1.exon2;Parent=MS.gene063026.t1
chr4.2	CDS	34974813	34975072	34974813	ID=cds.MS.gene063026.t1;Parent=MS.gene063026.t1
chr4.2	exon	34975470	34975620	34975470	ID=MS.gene063026.t1.exon3;Parent=MS.gene063026.t1
chr4.2	CDS	34975470	34975620	34975470	ID=cds.MS.gene063026.t1;Parent=MS.gene063026.t1

Gene Sequence

>MS.gene063026

ATGGCGATGGCTTTCAAAATGGTACTTCTTTACCTTTTAAATCATCTCACTCATATTAGAGTGTGTGTGATTTTACTTTGTTCTATATATTATTATATTTACATCATATGTTTAATATTGATGAATTTTGTTTTGTTTATACACATATTTATGGTAGATAAAATTGTGAATTAACAAATAATATGTTTGTTTATACACATATTTATGGTAGATAAAATTGTGAATTAACAAATAATATGAATCTGAACAGGCTACTACTGGGATGTGGGTAACAGATGAGTGCAAGAACTCTTTCATGGAGATGAAATGGAAGAAGGTGCATAGGTACATAGTTTTCAAGATCGATGAAAAAACAAGGCTTGTGACCGTTGACAAGGTTGGTGGTCCTGGAGAAAACTATGATGATCTTGCTGCTTCTTTGCCTAATGATGATTGTAGATATGCTGTCTTTGATTTTGATTTTGTCACTGTTGACAACTGCCGCAAGAGCAAGATCTTCTTCATTGCATGGTATAGTTCTCATTTCTCATCAATCTCAATTGTTAGATTAATCCAATAACAATGTTTGCTCAATGTCCTTTGTTTAATCCAACAATTGAAATTGGACTCTACAAAGCTGTGGCGCCGTAGACGATCTAATTGTGTGTGTTTGGATTGACGATGAGTTTGTCAGAATTTCTGTCAATCTAGACCTATACTAAATTGATTTTTTTTTTTACTTTTACTTGGTAAGTACCAAGTACTAGTAGTACAATTTAATGATATATGAGTCTAATACAAGCTGGCCCAATAATACCATATGATGTGATGAATGTACAATTTTAATTTCAATTATTATAAGATGATGATGATGATGATGATGATGATGAATGTGAATGTGAGGGATAATTTTGGGTAAAATTTTCAGGTCACCAACAGCATCAAGAATAAGAGAAAAGATACTATACGCAACATCAAAGGATGGGTTAAGAAGAGCTTTGGATGGAATCTCATATGAACTCCAAGCAACCGATCCAACTGAGATGGGATTCGATGTAATTCAAGACAGAGCCAAATAG

Protein sequence

>MS.gene063026.t1

MAMAFKMATTGMWVTDECKNSFMEMKWKKVHRYIVFKIDEKTRLVTVDKVGGPGENYDDLAASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIREKILYATSKDGLRRALDGISYELQATDPTEMGFDVIQDRAK