AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004357

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004357.t1 MTR_2g018260 97.778 225 5 0 1 225 1 225 1.53e-167 461
MS.gene004357.t1 MTR_1g045130 56.742 178 72 4 50 225 31 205 6.12e-67 205
MS.gene004357.t1 MTR_6g029230 37.107 159 98 1 40 198 40 196 4.51e-36 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004357.t1 AT4G18040 63.115 244 62 3 1 225 1 235 3.32e-110 316
MS.gene004357.t1 AT1G29550 56.846 241 87 3 1 225 1 240 4.96e-96 280
MS.gene004357.t1 AT1G29590 59.091 220 87 2 6 225 24 240 1.88e-95 279
MS.gene004357.t1 AT5G35620 49.738 191 88 6 30 214 5 193 3.79e-59 185
MS.gene004357.t1 AT5G35620 49.738 191 88 6 30 214 5 193 3.79e-59 185
MS.gene004357.t1 AT5G35620 50.303 165 74 6 30 188 5 167 3.17e-49 159
MS.gene004357.t1 AT5G18110 37.584 149 93 0 50 198 43 191 9.21e-32 115

Find 52 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAATTCTTGGACTTTC+TGG 0.032991 2.4:+67757694 MS.gene004357:CDS
CAAATAGGGTCTTCCCATTT+TGG 0.162954 2.4:-67758793 None:intergenic
ATTTGATCGTCGATGGATTT+TGG 0.183393 2.4:-67757558 None:intergenic
CTCACCCTCTTGAGAATTCT+TGG 0.186379 2.4:+67757685 MS.gene004357:CDS
AGCATAAGATTGAACCAAAA+TGG 0.214116 2.4:+67758779 MS.gene004357:CDS
TTTCTACTGTTGAAGAGTTT+TGG 0.268111 2.4:+67757784 MS.gene004357:CDS
TTTGTTCTAATGGTGGAAAA+TGG 0.298394 2.4:+67758812 MS.gene004357:CDS
TTTCCACCATTAGAACAAAT+AGG 0.308921 2.4:-67758808 None:intergenic
GTGACCAGCGGTGAGGGGTT+TGG 0.356180 2.4:-67757658 None:intergenic
CTTTAGTTGTTGGCAATGAT+TGG 0.357757 2.4:+67758982 MS.gene004357:intron
TTCCACCATTAGAACAAATA+GGG 0.371291 2.4:-67758807 None:intergenic
GAAGACCCTATTTGTTCTAA+TGG 0.377287 2.4:+67758802 MS.gene004357:CDS
ACTACCCCAAGCTTGTTGTT+TGG 0.381546 2.4:-67757739 None:intergenic
TAAATCCAAACAACAAGCTT+GGG 0.395339 2.4:+67757734 MS.gene004357:CDS
TGATACTAGCTGGTTGTATA+CGG 0.414513 2.4:+67758861 MS.gene004357:CDS
AGCGGTGAGGGGTTTGGAAA+TGG 0.424738 2.4:-67757652 None:intergenic
CATCCTAGCAAGTTGGTTGT+TGG 0.433951 2.4:+67758736 MS.gene004357:CDS
GTTGTTTGGATTTAGCTTGA+GGG 0.436316 2.4:-67757725 None:intergenic
AATTCTCAAGAGGGTGAGAT+TGG 0.439429 2.4:-67757680 None:intergenic
TGCTGCTAATGAAGCTGCTC+AGG 0.454593 2.4:+67759092 MS.gene004357:CDS
CTTGATTATAATGAAACCAT+TGG 0.456939 2.4:+67760155 MS.gene004357:CDS
GCATAAGATTGAACCAAAAT+GGG 0.459310 2.4:+67758780 MS.gene004357:CDS
GTTTGTGATTTGATCGTCGA+TGG 0.477672 2.4:-67757565 None:intergenic
CAATCTTATGCTTGAAACAA+TGG 0.479043 2.4:-67758768 None:intergenic
GATCATGGAGATGAAATTTG+TGG 0.479695 2.4:+67759015 MS.gene004357:CDS
ATTGGAGAGCAATTTGATCA+TGG 0.480040 2.4:+67759000 MS.gene004357:CDS
TGTTGTTTGGATTTAGCTTG+AGG 0.483575 2.4:-67757726 None:intergenic
AGATTGGTGACCAGCGGTGA+GGG 0.491646 2.4:-67757664 None:intergenic
CTAAATCCAAACAACAAGCT+TGG 0.494420 2.4:+67757733 MS.gene004357:CDS
GTGGAGCAGTTGTGAATGTC+AGG 0.501928 2.4:+67759034 MS.gene004357:CDS
CATTCACCATCCTAGCAAGT+TGG 0.529033 2.4:+67758729 MS.gene004357:CDS
CAGTTGTGAATGTCAGGAGT+AGG 0.531108 2.4:+67759040 MS.gene004357:CDS
GGGGAAAATCTGATACTAGC+TGG 0.537034 2.4:+67758851 MS.gene004357:CDS
CAACAACCAACTTGCTAGGA+TGG 0.538429 2.4:-67758735 None:intergenic
ATTTCCAAACCCCTCACCGC+TGG 0.539864 2.4:+67757654 MS.gene004357:CDS
ATCAACAACGATCTCGAAGA+AGG 0.542418 2.4:+67757603 MS.gene004357:CDS
GTTATATTTCAGGTGAGCAT+CGG 0.549012 2.4:+67760113 MS.gene004357:intron
GACCCTATTTGTTCTAATGG+TGG 0.552323 2.4:+67758805 MS.gene004357:CDS
GAGATTGGTGACCAGCGGTG+AGG 0.566288 2.4:-67757665 None:intergenic
TACATGGAATATAAAGCCAA+TGG 0.575752 2.4:-67760171 None:intergenic
GCTCCAACAACCAACTTGCT+AGG 0.578469 2.4:-67758739 None:intergenic
AGTTGTGAATGTCAGGAGTA+GGG 0.586788 2.4:+67759041 MS.gene004357:CDS
GAGGGGTTTGGAAATGGCGG+AGG 0.595654 2.4:-67757646 None:intergenic
GAGCATCGGAAAACAGTGGA+AGG 0.603606 2.4:+67760127 MS.gene004357:CDS
GGTGAGGGGTTTGGAAATGG+CGG 0.611632 2.4:-67757649 None:intergenic
GATTGGTGACCAGCGGTGAG+GGG 0.625908 2.4:-67757663 None:intergenic
AAAGTCCAAGAATTCTCAAG+AGG 0.638231 2.4:-67757690 None:intergenic
AAGTCCAAGAATTCTCAAGA+GGG 0.640086 2.4:-67757689 None:intergenic
AAATCCAAACAACAAGCTTG+GGG 0.655419 2.4:+67757735 MS.gene004357:CDS
AGGGTGAGATTGGTGACCAG+CGG 0.670042 2.4:-67757670 None:intergenic
AGGTGAGCATCGGAAAACAG+TGG 0.685728 2.4:+67760123 MS.gene004357:intron
TGTGAATGTCAGGAGTAGGG+CGG 0.715116 2.4:+67759044 MS.gene004357:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATGTTGAAGAATTTTTT+AGG + chr2.4:67758010-67758029 MS.gene004357:intron 15.0%
!!! AAATTAAGTTTTAAAATTGC+TGG - chr2.4:67759818-67759837 None:intergenic 15.0%
!!! AATGTTGAAGAATTTTTTTA+AGG + chr2.4:67758396-67758415 MS.gene004357:intron 15.0%
!!! AATTAAGTTTTAAAATTGCT+GGG - chr2.4:67759817-67759836 None:intergenic 15.0%
!!! TGATTCATTTTTAGTTAATT+GGG + chr2.4:67757973-67757992 MS.gene004357:intron 15.0%
!! AAGTTCAAGAGTAAATTATA+TGG - chr2.4:67759973-67759992 None:intergenic 20.0%
!! AATTGTAAACAATAAGGTAT+TGG - chr2.4:67759346-67759365 None:intergenic 20.0%
!! AGTTTGAATTGTAAACAATA+AGG - chr2.4:67759352-67759371 None:intergenic 20.0%
!! ATAAACTTCTGTTATATTTC+AGG + chr2.4:67760103-67760122 MS.gene004357:intron 20.0%
!! ATTTCTATAAGCTATATTTG+AGG + chr2.4:67758210-67758229 MS.gene004357:intron 20.0%
!! CTACAAAAAAATAGCTTATA+TGG - chr2.4:67759874-67759893 None:intergenic 20.0%
!! GAATTAGATGGTTATTATAA+TGG + chr2.4:67759234-67759253 MS.gene004357:intron 20.0%
!! GATTGAGAATTTAAATTTCA+GGG + chr2.4:67759748-67759767 MS.gene004357:intron 20.0%
!! TGATTGAGAATTTAAATTTC+AGG + chr2.4:67759747-67759766 MS.gene004357:intron 20.0%
!!! AAGATTCAGTTTTTATGAAT+TGG + chr2.4:67758312-67758331 MS.gene004357:intron 20.0%
!!! AAGATTCATTTTTTGTTTCT+TGG + chr2.4:67757884-67757903 MS.gene004357:intron 20.0%
!!! AATTCAATCTTTTCAACTTT+AGG + chr2.4:67758598-67758617 MS.gene004357:intron 20.0%
!!! AATTCTATCTTTTTCACTTT+TGG + chr2.4:67757814-67757833 MS.gene004357:intron 20.0%
!!! AATTGGGTATTTTTTACATT+AGG + chr2.4:67757945-67757964 MS.gene004357:intron 20.0%
!!! AATTTAGTCTGAAATTATCA+TGG + chr2.4:67759713-67759732 MS.gene004357:intron 20.0%
!!! AGATTCAGTTTTTATGAATT+GGG + chr2.4:67758313-67758332 MS.gene004357:intron 20.0%
!!! AGATTCATTTTTTGTTTCTT+GGG + chr2.4:67757885-67757904 MS.gene004357:intron 20.0%
!!! ATTGGGTATTTTTTACATTA+GGG + chr2.4:67757946-67757965 MS.gene004357:intron 20.0%
!!! ATTGTAAACAATAAGGTATT+GGG - chr2.4:67759345-67759364 None:intergenic 20.0%
!!! ATTGTTCATTTATTGATTCT+TGG + chr2.4:67758641-67758660 MS.gene004357:intron 20.0%
!!! ATTGTTCATTTATTGTTTCT+TGG + chr2.4:67757856-67757875 MS.gene004357:intron 20.0%
!!! GTGATTCATTTTTAGTTAAT+TGG + chr2.4:67757972-67757991 MS.gene004357:intron 20.0%
!!! TGATGAATTAAAGTACTAAT+TGG + chr2.4:67758932-67758951 MS.gene004357:intron 20.0%
! AACCATCTAATTCTTTCTTT+GGG - chr2.4:67759227-67759246 None:intergenic 25.0%
! AAGATACATATGGTAATTTC+TGG - chr2.4:67760028-67760047 None:intergenic 25.0%
! CAAGCATAAAAAGATACATA+TGG - chr2.4:67760038-67760057 None:intergenic 25.0%
! CTTGATTATAATGAAACCAT+TGG + chr2.4:67760155-67760174 MS.gene004357:CDS 25.0%
! TAACCATCTAATTCTTTCTT+TGG - chr2.4:67759228-67759247 None:intergenic 25.0%
! TATGCAATCTTATACAAAAG+CGG - chr2.4:67758132-67758151 None:intergenic 25.0%
! TTTATAGACTTTGGTCAATT+TGG + chr2.4:67759614-67759633 MS.gene004357:intron 25.0%
!! AATTGGGTATTTTTCACATT+AGG + chr2.4:67758329-67758348 MS.gene004357:intron 25.0%
!! ATCGTTCATTTATTGTTTCT+TGG + chr2.4:67758518-67758537 MS.gene004357:intron 25.0%
!! ATTGGGTATTTTTCACATTA+GGG + chr2.4:67758330-67758349 MS.gene004357:intron 25.0%
!! TTTTCAACTTTAGGTCTTTT+GGG + chr2.4:67758607-67758626 MS.gene004357:intron 25.0%
!!! AAGATACAGTTTTAGTGAAT+TGG + chr2.4:67757928-67757947 MS.gene004357:intron 25.0%
!!! AGATACAGTTTTAGTGAATT+GGG + chr2.4:67757929-67757948 MS.gene004357:intron 25.0%
!!! CTAGTGAATTTGTTTTATGA+GGG + chr2.4:67758888-67758907 MS.gene004357:intron 25.0%
!!! CTTCTTACTTTTGATTCAAT+AGG + chr2.4:67759124-67759143 MS.gene004357:intron 25.0%
!!! CTTGGTTATTTTTGTATCTT+GGG + chr2.4:67758536-67758555 MS.gene004357:intron 25.0%
!!! TCTTGGGTGTTTTTAATTTT+GGG + chr2.4:67757901-67757920 MS.gene004357:intron 25.0%
!!! TCTTGGTTATTTTTGTATCT+TGG + chr2.4:67758535-67758554 MS.gene004357:intron 25.0%
!!! TTCTTGGGTGTTTTTAATTT+TGG + chr2.4:67757900-67757919 MS.gene004357:intron 25.0%
AACTTCAGAGACTATATTAG+TGG - chr2.4:67759596-67759615 None:intergenic 30.0%
AATAAGCTCTTTATCCAAAC+AGG - chr2.4:67758064-67758083 None:intergenic 30.0%
ACCATCTAATTCTTTCTTTG+GGG - chr2.4:67759226-67759245 None:intergenic 30.0%
AGCATAAGATTGAACCAAAA+TGG + chr2.4:67758779-67758798 MS.gene004357:CDS 30.0%
CAAAAAGATCTAACTGATTG+AGG - chr2.4:67759686-67759705 None:intergenic 30.0%
CAATCTTATGCTTGAAACAA+TGG - chr2.4:67758771-67758790 None:intergenic 30.0%
CAGTTCGTGAACAATTTAAA+TGG + chr2.4:67759271-67759290 MS.gene004357:intron 30.0%
GAATCTTCTGATTGAACATT+TGG + chr2.4:67760067-67760086 MS.gene004357:intron 30.0%
GAGGACTTATGAAAATAAGT+TGG + chr2.4:67758229-67758248 MS.gene004357:intron 30.0%
GCATAAGATTGAACCAAAAT+GGG + chr2.4:67758780-67758799 MS.gene004357:CDS 30.0%
GTAAAATGTTGCAGAAATTG+AGG + chr2.4:67758571-67758590 MS.gene004357:intron 30.0%
GTTCTTCATCAATACCTTAT+TGG + chr2.4:67759560-67759579 MS.gene004357:intron 30.0%
TAAATCCAAACAACAAGCTT+GGG + chr2.4:67757734-67757753 MS.gene004357:CDS 30.0%
TACATGGAATATAAAGCCAA+TGG - chr2.4:67760174-67760193 None:intergenic 30.0%
TCTGATTGAACATTTGGTAA+TGG + chr2.4:67760073-67760092 MS.gene004357:intron 30.0%
TTCCACCATTAGAACAAATA+GGG - chr2.4:67758810-67758829 None:intergenic 30.0%
TTGATTCAATAGGAACTAGA+TGG + chr2.4:67759134-67759153 MS.gene004357:intron 30.0%
TTTCCACCATTAGAACAAAT+AGG - chr2.4:67758811-67758830 None:intergenic 30.0%
! AACAGTAGAAAAAGTGTAGA+CGG - chr2.4:67757775-67757794 None:intergenic 30.0%
! AACTCTTGTAATTTGTCAGA+TGG + chr2.4:67759907-67759926 MS.gene004357:intron 30.0%
! AGCTTCAATCTTTTCACTTT+AGG + chr2.4:67758036-67758055 MS.gene004357:intron 30.0%
! CTCAATCAGTTAGATCTTTT+TGG + chr2.4:67759684-67759703 MS.gene004357:intron 30.0%
! CTGATTGAACATTTGGTAAT+GGG + chr2.4:67760074-67760093 MS.gene004357:intron 30.0%
! CTTTTCAACTTTAGGTCTTT+TGG + chr2.4:67758606-67758625 MS.gene004357:intron 30.0%
! GCTTCAATCTTTTCACTTTA+GGG + chr2.4:67758037-67758056 MS.gene004357:intron 30.0%
! TAGTCTGAAATTATCATGGA+GGG + chr2.4:67759717-67759736 MS.gene004357:intron 30.0%
! TGTTGATGTCTTCGATTTTA+GGG - chr2.4:67757590-67757609 None:intergenic 30.0%
! TTAGTCTGAAATTATCATGG+AGG + chr2.4:67759716-67759735 MS.gene004357:intron 30.0%
! TTCTTGGTAACTTTAGTTGT+TGG + chr2.4:67758972-67758991 MS.gene004357:intron 30.0%
! TTGAGGATTTTCAATATCAG+AGG - chr2.4:67759669-67759688 None:intergenic 30.0%
! TTGTTGATGTCTTCGATTTT+AGG - chr2.4:67757591-67757610 None:intergenic 30.0%
! TTTCTACTGTTGAAGAGTTT+TGG + chr2.4:67757784-67757803 MS.gene004357:CDS 30.0%
! TTTGTTCTAATGGTGGAAAA+TGG + chr2.4:67758812-67758831 MS.gene004357:CDS 30.0%
!! AATCTTTTGAGAGACTGTTT+AGG - chr2.4:67758298-67758317 None:intergenic 30.0%
!! TCTCTGAAGTTTATAGACTT+TGG + chr2.4:67759605-67759624 MS.gene004357:intron 30.0%
!! TGATTGAACATTTGGTAATG+GGG + chr2.4:67760075-67760094 MS.gene004357:intron 30.0%
!! TTGAATTGTGCTATTTCTGT+AGG + chr2.4:67760256-67760275 MS.gene004357:intron 30.0%
!! TTGTGTTGCTGAAATTTTCA+TGG + chr2.4:67758685-67758704 MS.gene004357:intron 30.0%
!! TTTTTCACTTTTGGTCTTCT+GGG + chr2.4:67757823-67757842 MS.gene004357:intron 30.0%
!!! GCTAGTGAATTTGTTTTATG+AGG + chr2.4:67758887-67758906 MS.gene004357:intron 30.0%
!!! TTTTAAAATTGCTGGGAGTT+AGG - chr2.4:67759810-67759829 None:intergenic 30.0%
AAAGTCCAAGAATTCTCAAG+AGG - chr2.4:67757693-67757712 None:intergenic 35.0%
AAATCCAAACAACAAGCTTG+GGG + chr2.4:67757735-67757754 MS.gene004357:CDS 35.0%
AAGTCCAAGAATTCTCAAGA+GGG - chr2.4:67757692-67757711 None:intergenic 35.0%
ACCCCAAAGAAAGAATTAGA+TGG + chr2.4:67759222-67759241 MS.gene004357:intron 35.0%
ATGAGCAGTTGTTATGTGAA+TGG + chr2.4:67759423-67759442 MS.gene004357:intron 35.0%
ATTGGAGAGCAATTTGATCA+TGG + chr2.4:67759000-67759019 MS.gene004357:CDS 35.0%
CTAAATCCAAACAACAAGCT+TGG + chr2.4:67757733-67757752 MS.gene004357:CDS 35.0%
CTTTAGTTGTTGGCAATGAT+TGG + chr2.4:67758982-67759001 MS.gene004357:intron 35.0%
GAAGACCCTATTTGTTCTAA+TGG + chr2.4:67758802-67758821 MS.gene004357:CDS 35.0%
GATCATGGAGATGAAATTTG+TGG + chr2.4:67759015-67759034 MS.gene004357:CDS 35.0%
GATTTAACATGAAGTGACAC+AGG - chr2.4:67760221-67760240 None:intergenic 35.0%
GGGCGGAAAAAATTTCTATT+TGG + chr2.4:67759061-67759080 MS.gene004357:CDS 35.0%
GTCATGAAAGAAAGTTAGCT+TGG - chr2.4:67759997-67760016 None:intergenic 35.0%
TGATACTAGCTGGTTGTATA+CGG + chr2.4:67758861-67758880 MS.gene004357:CDS 35.0%
TTGAGAATTCTTGGACTTTC+TGG + chr2.4:67757694-67757713 MS.gene004357:CDS 35.0%
! ACAGTAGAAAAAGTGTAGAC+GGG - chr2.4:67757774-67757793 None:intergenic 35.0%
! GCTTCGATCTTTTCACTTTA+AGG + chr2.4:67758423-67758442 MS.gene004357:intron 35.0%
! GTATCTTTTTATGCTTGTGC+TGG + chr2.4:67760041-67760060 MS.gene004357:intron 35.0%
! GTTATATTTCAGGTGAGCAT+CGG + chr2.4:67760113-67760132 MS.gene004357:intron 35.0%
! TCTTGTAATTTGTCAGATGG+AGG + chr2.4:67759910-67759929 MS.gene004357:intron 35.0%
! TTTCACTTTAAGGACCTGTT+TGG + chr2.4:67758433-67758452 MS.gene004357:intron 35.0%
!! ATGGACTGTGCAATTTTCTA+GGG + chr2.4:67758831-67758850 MS.gene004357:CDS 35.0%
!! ATTTGATCGTCGATGGATTT+TGG - chr2.4:67757561-67757580 None:intergenic 35.0%
!! CATGTGTGTGTTTTTGTTCT+TGG + chr2.4:67758956-67758975 MS.gene004357:intron 35.0%
!! CTTTTTCACTTTTGGTCTTC+TGG + chr2.4:67757822-67757841 MS.gene004357:intron 35.0%
!! GTTGTTTGGATTTAGCTTGA+GGG - chr2.4:67757728-67757747 None:intergenic 35.0%
!! TGTTGTTTGGATTTAGCTTG+AGG - chr2.4:67757729-67757748 None:intergenic 35.0%
!! TTTGATCGTCGATGGATTTT+GGG - chr2.4:67757560-67757579 None:intergenic 35.0%
!!! AATGGACTGTGCAATTTTCT+AGG + chr2.4:67758830-67758849 MS.gene004357:CDS 35.0%
AATTCTCAAGAGGGTGAGAT+TGG - chr2.4:67757683-67757702 None:intergenic 40.0%
AGTTGTGAATGTCAGGAGTA+GGG + chr2.4:67759041-67759060 MS.gene004357:CDS 40.0%
ATCAACAACGATCTCGAAGA+AGG + chr2.4:67757603-67757622 MS.gene004357:CDS 40.0%
GACCCTATTTGTTCTAATGG+TGG + chr2.4:67758805-67758824 MS.gene004357:CDS 40.0%
GTGGTTCACTCTAACCAATA+AGG - chr2.4:67759577-67759596 None:intergenic 40.0%
GTTTGTGATTTGATCGTCGA+TGG - chr2.4:67757568-67757587 None:intergenic 40.0%
TGGGAGAAAACAACAAACAC+AGG - chr2.4:67759326-67759345 None:intergenic 40.0%
! CAAATAGGGTCTTCCCATTT+TGG - chr2.4:67758796-67758815 None:intergenic 40.0%
! TGGACTGTGCAATTTTCTAG+GGG + chr2.4:67758832-67758851 MS.gene004357:CDS 40.0%
! TTTCACTTTAGGGACCTGTT+TGG + chr2.4:67758047-67758066 MS.gene004357:intron 40.0%
!! CTACTGTTGAAGAGTTTTGG+AGG + chr2.4:67757787-67757806 MS.gene004357:CDS 40.0%
!! TTGATCGTCGATGGATTTTG+GGG - chr2.4:67757559-67757578 None:intergenic 40.0%
ACTAGCTGGTTGTATACGGT+AGG + chr2.4:67758865-67758884 MS.gene004357:intron 45.0%
CAGTTGTGAATGTCAGGAGT+AGG + chr2.4:67759040-67759059 MS.gene004357:CDS 45.0%
CATTCACCATCCTAGCAAGT+TGG + chr2.4:67758729-67758748 MS.gene004357:CDS 45.0%
CTCACCCTCTTGAGAATTCT+TGG + chr2.4:67757685-67757704 MS.gene004357:CDS 45.0%
GGGGAAAATCTGATACTAGC+TGG + chr2.4:67758851-67758870 MS.gene004357:CDS 45.0%
TGACACAGGCATAAAAACGC+AGG - chr2.4:67760207-67760226 None:intergenic 45.0%
! CGCAGGCAGTTTTACTTACA+TGG - chr2.4:67760190-67760209 None:intergenic 45.0%
!! ACTACCCCAAGCTTGTTGTT+TGG - chr2.4:67757742-67757761 None:intergenic 45.0%
!! CAACAACCAACTTGCTAGGA+TGG - chr2.4:67758738-67758757 None:intergenic 45.0%
!! CATCCTAGCAAGTTGGTTGT+TGG + chr2.4:67758736-67758755 MS.gene004357:CDS 45.0%
GTGGAGCAGTTGTGAATGTC+AGG + chr2.4:67759034-67759053 MS.gene004357:CDS 50.0%
TGCTGCTAATGAAGCTGCTC+AGG + chr2.4:67759092-67759111 MS.gene004357:CDS 50.0%
TGTGAATGTCAGGAGTAGGG+CGG + chr2.4:67759044-67759063 MS.gene004357:CDS 50.0%
! GCTCCAACAACCAACTTGCT+AGG - chr2.4:67758742-67758761 None:intergenic 50.0%
!! AGGTGAGCATCGGAAAACAG+TGG + chr2.4:67760123-67760142 MS.gene004357:intron 50.0%
!! GAGCATCGGAAAACAGTGGA+AGG + chr2.4:67760127-67760146 MS.gene004357:CDS 50.0%
AGCGGTGAGGGGTTTGGAAA+TGG - chr2.4:67757655-67757674 None:intergenic 55.0%
ATTTCCAAACCCCTCACCGC+TGG + chr2.4:67757654-67757673 MS.gene004357:CDS 55.0%
GGTGAGGGGTTTGGAAATGG+CGG - chr2.4:67757652-67757671 None:intergenic 55.0%
! AGATTGGTGACCAGCGGTGA+GGG - chr2.4:67757667-67757686 None:intergenic 55.0%
! AGGGTGAGATTGGTGACCAG+CGG - chr2.4:67757673-67757692 None:intergenic 55.0%
GAGGGGTTTGGAAATGGCGG+AGG - chr2.4:67757649-67757668 None:intergenic 60.0%
! GAGATTGGTGACCAGCGGTG+AGG - chr2.4:67757668-67757687 None:intergenic 60.0%
! GATTGGTGACCAGCGGTGAG+GGG - chr2.4:67757666-67757685 None:intergenic 60.0%
GTGACCAGCGGTGAGGGGTT+TGG - chr2.4:67757661-67757680 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 67757540 67760328 67757540 ID=MS.gene004357
chr2.4 mRNA 67757540 67760328 67757540 ID=MS.gene004357.t1;Parent=MS.gene004357
chr2.4 exon 67757540 67757808 67757540 ID=MS.gene004357.t1.exon1;Parent=MS.gene004357.t1
chr2.4 CDS 67757540 67757808 67757540 ID=cds.MS.gene004357.t1;Parent=MS.gene004357.t1
chr2.4 exon 67758717 67758882 67758717 ID=MS.gene004357.t1.exon2;Parent=MS.gene004357.t1
chr2.4 CDS 67758717 67758882 67758717 ID=cds.MS.gene004357.t1;Parent=MS.gene004357.t1
chr2.4 exon 67758988 67759113 67758988 ID=MS.gene004357.t1.exon3;Parent=MS.gene004357.t1
chr2.4 CDS 67758988 67759113 67758988 ID=cds.MS.gene004357.t1;Parent=MS.gene004357.t1
chr2.4 exon 67760125 67760190 67760125 ID=MS.gene004357.t1.exon4;Parent=MS.gene004357.t1
chr2.4 CDS 67760125 67760190 67760125 ID=cds.MS.gene004357.t1;Parent=MS.gene004357.t1
chr2.4 exon 67760278 67760328 67760278 ID=MS.gene004357.t1.exon5;Parent=MS.gene004357.t1
chr2.4 CDS 67760278 67760328 67760278 ID=cds.MS.gene004357.t1;Parent=MS.gene004357.t1
Gene Sequence

>MS.gene004357

ATGGTTGTAGAAGAAACCCCAAAATCCATCGACGATCAAATCACAAACCCTAAAATCGAAGACATCAACAACGATCTCGAAGAAGGAGAGATCGACGACGATGATTCCTCCGCCATTTCCAAACCCCTCACCGCTGGTCACCAATCTCACCCTCTTGAGAATTCTTGGACTTTCTGGTTCGATAACCCTCAAGCTAAATCCAAACAACAAGCTTGGGGTAGTTCGATTCGACCCGTCTACACTTTTTCTACTGTTGAAGAGTTTTGGAGGTAAAAATTCTATCTTTTTCACTTTTGGTCTTCTGGGTCTTGAAATAATTGTTCATTTATTGTTTCTTGGATGAAAAGATTCATTTTTTGTTTCTTGGGTGTTTTTAATTTTGGGTCAAAAGATACAGTTTTAGTGAATTGGGTATTTTTTACATTAGGGTTTGTGATTCATTTTTAGTTAATTGGGTATTTCTTGTTTGTAAAATGTTGAAGAATTTTTTAGGTAAAGCTTCAATCTTTTCACTTTAGGGACCTGTTTGGATAAAGAGCTTATTTGCAGCTTATATCACAAGCGTTTATCAATATGCTTATCATGATATCCGCTTTTGTATAAGATTGCATAAGCTGTTTTATAGCAAAAAAATAATAACGCTGAATTGTTTTTTTATATGCTATAAGTTATTTCTATAAGCTATATTTGAGGACTTATGAAAATAAGTTGGAAAAAGCTTATGAAATTTGTCATAAGCTGTTTTCATAAATTCTCCTAAACAGTCTCTCAAAAGATTCAGTTTTTATGAATTGGGTATTTTTCACATTAGGGTTTGTGATTCATTTTTAGTTAATTGGGTATTTCTTGTTTGTACAATGTTGAAGAATTTTTTTAAGGTAACGCTTCGATCTTTTCACTTTAAGGACCTGTTTGGATAAAGAGCTTATTTGCAGCTTATATCATGATAAGCGCTTATGTATAAGCTTGCATAAAAATATCGTTCATTTATTGTTTCTTGGTTATTTTTGTATCTTGGGTATTTCTTGTTTGTAAAATGTTGCAGAAATTGAGGTAAAAATTCAATCTTTTCAACTTTAGGTCTTTTGGGTCATCAAAAATATTGTTCATTTATTGATTCTTGGATATTTTTGTTTCTTAGTTATTTGTGTTGCTGAAATTTTCATGGTTTTTTCAGCATTTACAATAACATTCACCATCCTAGCAAGTTGGTTGTTGGAGCTGATTTCCATTGTTTCAAGCATAAGATTGAACCAAAATGGGAAGACCCTATTTGTTCTAATGGTGGAAAATGGACTGTGCAATTTTCTAGGGGAAAATCTGATACTAGCTGGTTGTATACGGTAGGCTAGTGAATTTGTTTTATGAGGGTATTATTTTGATTATTCTTTGTGATGAATTAAAGTACTAATTGGTCATGTGTGTGTTTTTGTTCTTGGTAACTTTAGTTGTTGGCAATGATTGGAGAGCAATTTGATCATGGAGATGAAATTTGTGGAGCAGTTGTGAATGTCAGGAGTAGGGCGGAAAAAATTTCTATTTGGACTAAGAATGCTGCTAATGAAGCTGCTCAGGTAATTTCTTCTTCTTACTTTTGATTCAATAGGAACTAGATGGTTTTGATGTTGAAGAGTAATATTATGATTTTCAAACTAAAGATGTGCATTTGTTTTGTGACTGAGACCCCAAAGAAAGAATTAGATGGTTATTATAATGGCATGTATTCAATCACAGTTCGTGAACAATTTAAATGGACATTTTTTACATTTTTGTTGCTTCAGTGCCTGTGTTTGTTGTTTTCTCCCAATACCTTATTGTTTACAATTCAAACTATATGACTACTATGATGTTACTGAGTATTATTATGATTTTTCAAACTAATGATGAGCAGTTGTTATGTGAATGGCTCACTCTTATCATTGCTGTTAAATATGAATGTAAAAAGCATGTATTCACAGTTCATAAGCTATTTAAATAGACAGTTTTCACGTTTTTTGTTTATTCTATGTCTGTGTTTCTTGTTCTTCATCAATACCTTATTGGTTAGAGTGAACCACTAATATAGTCTCTGAAGTTTATAGACTTTGGTCAATTTGGCATCTGTAATTTACAAAATTTCTTAATGACCTCTGATATTGAAAATCCTCAATCAGTTAGATCTTTTTGGTCGATCAATTTAGTCTGAAATTATCATGGAGGGACTAATGTGATTGAGAATTTAAATTTCAGGGATCACTTGTGAATTTTATTGATTTTAAGACAAATTGCCTAACTCCCAGCAATTTTAAAACTTAATTTATGATTCACTATTATACATATGCTGCGACAATTCCATATAAGCTATTTTTTTGTAGAAGAGAATTTAAAAACTCTTGTAATTTGTCAGATGGAGGATGAATGTGTGATTATTATACATATGCTGCGACAATTCCATATAATTTACTCTTGAACTTGCCAAGCTAACTTTCTTTCATGACTTTTTAGACCAGAAATTACCATATGTATCTTTTTATGCTTGTGCTGGTGTGAATCTTCTGATTGAACATTTGGTAATGGGGAACATATAAACTTCTGTTATATTTCAGGTGAGCATCGGAAAACAGTGGAAGGAGTTTCTTGATTATAATGAAACCATTGGCTTTATATTCCATGTAAGTAAAACTGCCTGCGTTTTTATGCCTGTGTCACTTCATGTTAAATCACACTAACTTCTGTTTTGAATTGTGCTATTTCTGTAGGATGATGCAAAGAAGCTTGACAGAGCTGCTAAAAACAAATATGTTGTATGA
Protein sequence

>MS.gene004357.t1

MVVEETPKSIDDQITNPKIEDINNDLEEGEIDDDDSSAISKPLTAGHQSHPLENSWTFWFDNPQAKSKQQAWGSSIRPVYTFSTVEEFWSIYNNIHHPSKLVVGADFHCFKHKIEPKWEDPICSNGGKWTVQFSRGKSDTSWLYTLLAMIGEQFDHGDEICGAVVNVRSRAEKISIWTKNAANEAAQVSIGKQWKEFLDYNETIGFIFHDDAKKLDRAAKNKYVV