Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010167.t1 | XP_003615184.1 | 96.4 | 389 | 11 | 1 | 1 | 389 | 12 | 397 | 1.40E-176 | 629 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010167.t1 | Q9QYW0 | 29.5 | 312 | 179 | 9 | 88 | 360 | 183 | 492 | 9.9e-25 | 115.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010167.t1 | G7KAI8 | 96.4 | 389 | 11 | 1 | 1 | 389 | 12 | 397 | 1.0e-176 | 629.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene010167 | MS.gene003175 | PPI |
| MS.gene010167 | MS.gene003846 | PPI |
| MS.gene030707 | MS.gene010167 | PPI |
| MS.gene010167 | MS.gene28161 | PPI |
| MS.gene010167 | MS.gene065496 | PPI |
| MS.gene010167 | MS.gene65479 | PPI |
| MS.gene010167 | MS.gene88378 | PPI |
| MS.gene010167 | MS.gene28303 | PPI |
| MS.gene031356 | MS.gene010167 | PPI |
| MS.gene02854 | MS.gene010167 | PPI |
| MS.gene029825 | MS.gene010167 | PPI |
| MS.gene017174 | MS.gene010167 | PPI |
| MS.gene010167 | MS.gene063568 | PPI |
| MS.gene75013 | MS.gene010167 | PPI |
| MS.gene010167 | MS.gene038409 | PPI |
| MS.gene010167 | MS.gene45448 | PPI |
| MS.gene010167 | MS.gene58159 | PPI |
| MS.gene062301 | MS.gene010167 | PPI |
| MS.gene010167 | MS.gene056617 | PPI |
| MS.gene01984 | MS.gene010167 | PPI |
| MS.gene010167 | MS.gene048301 | PPI |
| MS.gene28304 | MS.gene010167 | PPI |
| MS.gene010167 | MS.gene053381 | PPI |
| MS.gene063209 | MS.gene010167 | PPI |
| MS.gene010167 | MS.gene70303 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010167.t1 | MTR_5g064850 | 96.401 | 389 | 11 | 1 | 1 | 389 | 12 | 397 | 0.0 | 768 |
| MS.gene010167.t1 | MTR_5g064850 | 96.144 | 389 | 10 | 2 | 1 | 389 | 12 | 395 | 0.0 | 759 |
| MS.gene010167.t1 | MTR_7g007890 | 94.970 | 338 | 17 | 0 | 52 | 389 | 60 | 397 | 0.0 | 669 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010167.t1 | AT5G61330 | 59.838 | 371 | 135 | 3 | 18 | 388 | 80 | 436 | 7.26e-144 | 416 |
| MS.gene010167.t1 | AT5G61330 | 59.838 | 371 | 135 | 3 | 18 | 388 | 80 | 436 | 7.26e-144 | 416 |
| MS.gene010167.t1 | AT5G61330 | 59.838 | 371 | 135 | 3 | 18 | 388 | 91 | 447 | 9.92e-144 | 416 |
Find 74 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCACAGCTTGTCCTTTC+AGG | 0.086562 | 5.2:-54798471 | None:intergenic |
| TGAAAAGATAGTAAACTTTA+TGG | 0.146420 | 5.2:+54804169 | MS.gene010167:CDS |
| CTTGCAGTATGGCAACTTTC+AGG | 0.156523 | 5.2:+54801445 | MS.gene010167:intron |
| AGATCAGCTATTAAAATATT+TGG | 0.170387 | 5.2:+54801706 | MS.gene010167:CDS |
| ACTGGTGCTGCTGCAATTAA+AGG | 0.238779 | 5.2:+54801510 | MS.gene010167:CDS |
| TTTAGAAATTTCTAATGATT+TGG | 0.246736 | 5.2:+54800897 | MS.gene010167:CDS |
| GGAGACTTTGGATTCTATAT+TGG | 0.273642 | 5.2:+54798972 | MS.gene010167:CDS |
| CATCTGCTTGATCATTCTAC+TGG | 0.336534 | 5.2:-54801674 | None:intergenic |
| ACAACTTGAGAAAGAGTATA+TGG | 0.343510 | 5.2:+54796765 | MS.gene010167:CDS |
| CATGAAGAAATCACCATTAC+TGG | 0.347783 | 5.2:-54799538 | None:intergenic |
| AACTTCTTCCAAGGAGACTT+TGG | 0.353164 | 5.2:+54798960 | MS.gene010167:CDS |
| TCATATAAGCAGCAACTTGA+TGG | 0.354843 | 5.2:-54801645 | None:intergenic |
| GGACGGTGATCCTGAACTTC+TGG | 0.355336 | 5.2:+54802563 | MS.gene010167:CDS |
| ATGCTATTCGATGATCTGTT+TGG | 0.371540 | 5.2:+54804227 | MS.gene010167:CDS |
| AGAAGATGATGGAGAGGTTG+AGG | 0.372751 | 5.2:+54796678 | MS.gene010167:CDS |
| TCAGAATCGTCCAGAAGTTC+AGG | 0.374459 | 5.2:-54802573 | None:intergenic |
| TAGTGAGGTGGAGGAGGAAG+AGG | 0.395864 | 5.2:+54796654 | MS.gene010167:CDS |
| AGAAGAAGATGAGGGACAAA+AGG | 0.412567 | 5.2:+54796714 | MS.gene010167:CDS |
| GCCATGGGAGGAAGATTTGC+CGG | 0.414884 | 5.2:-54804200 | None:intergenic |
| TGATGATAGTGAGGTGGAGG+AGG | 0.415199 | 5.2:+54796648 | MS.gene010167:CDS |
| AAGAATTGTTACCTTGTTGA+AGG | 0.419515 | 5.2:-54796544 | None:intergenic |
| GAAGAACTTGAAGCATCATA+AGG | 0.425208 | 5.2:+54798438 | MS.gene010167:CDS |
| GAATCAGATGAGACAGTTAG+AGG | 0.431696 | 5.2:+54798919 | MS.gene010167:CDS |
| TTGCAGTATGGCAACTTTCA+GGG | 0.436730 | 5.2:+54801446 | MS.gene010167:intron |
| AGCCATTCTCAAGTTCAAAT+AGG | 0.443809 | 5.2:+54798774 | MS.gene010167:CDS |
| AAACAGAAACCACACCTTCT+GGG | 0.444736 | 5.2:-54798494 | None:intergenic |
| ATGACAATCTTGATCAAGAA+TGG | 0.451953 | 5.2:+54800920 | MS.gene010167:CDS |
| GAAGATGATGGAGAGGTTGA+GGG | 0.453482 | 5.2:+54796679 | MS.gene010167:CDS |
| TGGATGGAAAGATGATGAAA+TGG | 0.453888 | 5.2:+54796741 | MS.gene010167:CDS |
| ACAATTGATCCTTCATCATC+AGG | 0.454754 | 5.2:+54802630 | MS.gene010167:CDS |
| AGCCTATTTGAACTTGAGAA+TGG | 0.465447 | 5.2:-54798776 | None:intergenic |
| GGAGGAAGATTTGCCGGCCG+AGG | 0.466535 | 5.2:-54804194 | None:intergenic |
| TATAGAATCCAAAGTCTCCT+TGG | 0.480413 | 5.2:-54798968 | None:intergenic |
| TAATGATAGCTCTAATGAAC+CGG | 0.480771 | 5.2:+54801833 | MS.gene010167:CDS |
| ATCGAATAGCATTGGTGCCA+TGG | 0.493599 | 5.2:-54804216 | None:intergenic |
| AAACTTTATGGCTCCTCGGC+CGG | 0.497362 | 5.2:+54804181 | MS.gene010167:CDS |
| GATGAGGGACAAAAGGAAAG+TGG | 0.500545 | 5.2:+54796721 | MS.gene010167:CDS |
| AGGATGACACAAGTGACAAC+TGG | 0.501309 | 5.2:+54801492 | MS.gene010167:CDS |
| AAACTCACAGCTCTTGCTCC+TGG | 0.502870 | 5.2:-54796798 | None:intergenic |
| AATTGTTTACCTGATGATGA+AGG | 0.510261 | 5.2:-54802639 | None:intergenic |
| GCCGGCAAATCTTCCTCCCA+TGG | 0.513552 | 5.2:+54804199 | MS.gene010167:CDS |
| TAATGATGATAGTGAGGTGG+AGG | 0.516445 | 5.2:+54796645 | MS.gene010167:CDS |
| GAGGAAGAGGAAGAAGATGA+TGG | 0.516558 | 5.2:+54796667 | MS.gene010167:CDS |
| AGAGGAAGAAGATGATGGAG+AGG | 0.518108 | 5.2:+54796672 | MS.gene010167:CDS |
| ACCATTACTGGCTTGAGCGA+TGG | 0.525229 | 5.2:-54799526 | None:intergenic |
| GGGACAAGTCAATCAACAAA+TGG | 0.528981 | 5.2:+54801466 | MS.gene010167:CDS |
| TGACAATCTTGATCAAGAAT+GGG | 0.532967 | 5.2:+54800921 | MS.gene010167:CDS |
| CGATGAGGAAGAAGAAGATG+AGG | 0.533720 | 5.2:+54796705 | MS.gene010167:CDS |
| AGGGACAAAAGGAAAGTGGA+TGG | 0.542308 | 5.2:+54796725 | MS.gene010167:CDS |
| TTACTGGCTTGAGCGATGGA+TGG | 0.545707 | 5.2:-54799522 | None:intergenic |
| AGATTTCTCAGATGCACAGC+CGG | 0.551895 | 5.2:+54800947 | MS.gene010167:CDS |
| ATCTGCTTGATCATTCTACT+GGG | 0.552705 | 5.2:-54801673 | None:intergenic |
| AACAGATCATCGAATAGCAT+TGG | 0.555701 | 5.2:-54804224 | None:intergenic |
| TCGAATAGCATTGGTGCCAT+GGG | 0.559495 | 5.2:-54804215 | None:intergenic |
| CACGGCCAACCGTGATTACG+AGG | 0.561299 | 5.2:-54804279 | None:intergenic |
| TTCTATATTGGAACTGCAAG+AGG | 0.562013 | 5.2:+54798984 | MS.gene010167:CDS |
| AGATTTGATAACTTCTTCCA+AGG | 0.564786 | 5.2:+54798951 | MS.gene010167:CDS |
| GGTTAATGATGATAGTGAGG+TGG | 0.576958 | 5.2:+54796642 | MS.gene010167:intron |
| TAGTAAACTTTATGGCTCCT+CGG | 0.577305 | 5.2:+54804177 | MS.gene010167:CDS |
| AGAGGTTGAGGGAAGCGATG+AGG | 0.589125 | 5.2:+54796690 | MS.gene010167:CDS |
| TCCATCGCTCAAGCCAGTAA+TGG | 0.594131 | 5.2:+54799525 | MS.gene010167:CDS |
| GTATATGGATCTTCATCACC+AGG | 0.598970 | 5.2:+54796780 | MS.gene010167:CDS |
| ACAAGCTGTGAAAGCCCAGA+AGG | 0.601029 | 5.2:+54798480 | MS.gene010167:CDS |
| AAGGATGAAGATCTCCTGAA+AGG | 0.606031 | 5.2:+54798457 | MS.gene010167:CDS |
| AAGTTCAAATAGGCTACCCC+AGG | 0.608312 | 5.2:+54798784 | MS.gene010167:CDS |
| GTAGGTTAATGATGATAGTG+AGG | 0.609411 | 5.2:+54796639 | MS.gene010167:intron |
| AATAGCATTGGTGCCATGGG+AGG | 0.612836 | 5.2:-54804212 | None:intergenic |
| ATTAGAGCTATCATTAACCT+CGG | 0.615774 | 5.2:-54801826 | None:intergenic |
| CTTATGTCCCCAGGTTGCCG+AGG | 0.632820 | 5.2:+54801809 | MS.gene010167:intron |
| AGTCAATCAACAAATGGCAG+AGG | 0.633983 | 5.2:+54801472 | MS.gene010167:CDS |
| TGATGATATGTCCTTCAACA+AGG | 0.635737 | 5.2:+54796533 | MS.gene010167:CDS |
| GATGAGGAAGAAGAAGATGA+GGG | 0.660997 | 5.2:+54796706 | MS.gene010167:CDS |
| TTGTGCTCAGGAAACACATG+TGG | 0.674699 | 5.2:+54802542 | MS.gene010167:intron |
| GCTCAGGAAACACATGTGGA+CGG | 0.754092 | 5.2:+54802546 | MS.gene010167:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 54796498 | 54804286 | 54796498 | ID=MS.gene010167 |
| chr5.2 | mRNA | 54796498 | 54804286 | 54796498 | ID=MS.gene010167.t1;Parent=MS.gene010167 |
| chr5.2 | exon | 54796498 | 54796554 | 54796498 | ID=MS.gene010167.t1.exon1;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54796498 | 54796554 | 54796498 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54796643 | 54796812 | 54796643 | ID=MS.gene010167.t1.exon2;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54796643 | 54796812 | 54796643 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54798429 | 54798501 | 54798429 | ID=MS.gene010167.t1.exon3;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54798429 | 54798501 | 54798429 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54798731 | 54798805 | 54798731 | ID=MS.gene010167.t1.exon4;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54798731 | 54798805 | 54798731 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54798895 | 54799005 | 54798895 | ID=MS.gene010167.t1.exon5;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54798895 | 54799005 | 54798895 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54799504 | 54799546 | 54799504 | ID=MS.gene010167.t1.exon6;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54799504 | 54799546 | 54799504 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54800875 | 54800968 | 54800875 | ID=MS.gene010167.t1.exon7;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54800875 | 54800968 | 54800875 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54801452 | 54801554 | 54801452 | ID=MS.gene010167.t1.exon8;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54801452 | 54801554 | 54801452 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54801637 | 54801732 | 54801637 | ID=MS.gene010167.t1.exon9;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54801637 | 54801732 | 54801637 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54801822 | 54801854 | 54801822 | ID=MS.gene010167.t1.exon10;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54801822 | 54801854 | 54801822 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54802552 | 54802651 | 54802552 | ID=MS.gene010167.t1.exon11;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54802552 | 54802651 | 54802552 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54803975 | 54804059 | 54803975 | ID=MS.gene010167.t1.exon12;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54803975 | 54804059 | 54803975 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| chr5.2 | exon | 54804157 | 54804286 | 54804157 | ID=MS.gene010167.t1.exon13;Parent=MS.gene010167.t1 |
| chr5.2 | CDS | 54804157 | 54804286 | 54804157 | ID=cds.MS.gene010167.t1;Parent=MS.gene010167.t1 |
| Gene Sequence |
| Protein sequence |
