Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28161.t1 | XP_003630123.2 | 98.9 | 1010 | 11 | 0 | 1 | 1010 | 1 | 1010 | 0.00E+00 | 1975.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28161.t1 | Q9XIK4 | 71.8 | 1021 | 277 | 6 | 1 | 1015 | 1 | 1016 | 0.0e+00 | 1437.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28161.t1 | G7LBB9 | 98.9 | 1010 | 11 | 0 | 1 | 1010 | 1 | 1010 | 0.0e+00 | 1975.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056257 | MS.gene28161 | 0.804396 | 2.20E-49 | -1.69E-46 |
| MS.gene058030 | MS.gene28161 | 0.835312 | 1.81E-56 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28161.t1 | MTR_8g092060 | 98.931 | 1029 | 11 | 0 | 1 | 1029 | 1 | 1029 | 0.0 | 2113 |
| MS.gene28161.t1 | MTR_2g028030 | 74.951 | 1030 | 229 | 9 | 1 | 1026 | 1 | 1005 | 0.0 | 1551 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28161.t1 | AT1G10490 | 71.609 | 1032 | 282 | 6 | 1 | 1026 | 1 | 1027 | 0.0 | 1468 |
| MS.gene28161.t1 | AT1G10490 | 71.609 | 1032 | 282 | 6 | 1 | 1026 | 1 | 1027 | 0.0 | 1468 |
| MS.gene28161.t1 | AT3G57940 | 70.257 | 1012 | 286 | 6 | 1 | 1008 | 1 | 1001 | 0.0 | 1413 |
| MS.gene28161.t1 | AT3G57940 | 69.960 | 1012 | 284 | 7 | 1 | 1008 | 1 | 996 | 0.0 | 1398 |
Find 219 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTTCCTTGATGCAATTT+TGG | 0.109454 | 4.4:+9659166 | MS.gene28161:CDS |
| TGGTATAACCTGCTAATATT+AGG | 0.153603 | 4.4:-9662916 | None:intergenic |
| GCTTTCGCAAGTTGAATTAT+TGG | 0.181367 | 4.4:+9661857 | MS.gene28161:CDS |
| TTCCTGTCTTCCACTGTTAA+TGG | 0.209425 | 4.4:+9661945 | MS.gene28161:CDS |
| CATCATTATTCAAGGGTTTC+AGG | 0.210462 | 4.4:-9666711 | None:intergenic |
| CCTTACAACAGTAGCATTCT+TGG | 0.213127 | 4.4:-9661633 | None:intergenic |
| GTCTTTGCTTGGCCCATATC+TGG | 0.233387 | 4.4:+9661920 | MS.gene28161:CDS |
| ATGAACCCACCCTGGTAAAA+TGG | 0.256257 | 4.4:-9666775 | None:intergenic |
| AATGACGACCCAGGGTCTTT+TGG | 0.257695 | 4.4:+9657292 | MS.gene28161:CDS |
| CCGTGGACCTCTTTCGCTTT+TGG | 0.262875 | 4.4:+9666512 | MS.gene28161:CDS |
| AATATTGTCTCTATTTATAA+AGG | 0.271442 | 4.4:+9668482 | MS.gene28161:CDS |
| CGATCCATGTTTGTCATTAT+CGG | 0.274182 | 4.4:+9656781 | MS.gene28161:CDS |
| AAGCATTCTGAAAGAGTTCT+TGG | 0.279878 | 4.4:+9657380 | MS.gene28161:CDS |
| GCAAAGATTTGCTAAACTTC+TGG | 0.287441 | 4.4:+9666884 | MS.gene28161:CDS |
| GGTATAACCTGCTAATATTA+GGG | 0.307074 | 4.4:-9662915 | None:intergenic |
| CTTCGGGATGTGTGTTCTTC+AGG | 0.309009 | 4.4:+9657409 | MS.gene28161:CDS |
| CATGACCACGCATGCCTTAC+AGG | 0.309529 | 4.4:-9657894 | None:intergenic |
| ATCAGAACTCTCATTGAAAA+TGG | 0.315497 | 4.4:+9656742 | MS.gene28161:CDS |
| CAGCCATAAATCAAGTAAAA+AGG | 0.320830 | 4.4:+9669202 | MS.gene28161:CDS |
| GACCTTATGTGAGATGAAAT+TGG | 0.324806 | 4.4:-9657938 | None:intergenic |
| GCATACCGCTGGAGCAATTC+AGG | 0.326482 | 4.4:-9669020 | None:intergenic |
| TTGTCAGCAGTTGTCTTGAT+AGG | 0.327701 | 4.4:-9667270 | None:intergenic |
| CAAATGAGGAAGATCTGTTC+TGG | 0.328217 | 4.4:-9666425 | None:intergenic |
| GGGACTATCAGAAGCTTATC+AGG | 0.328308 | 4.4:+9658945 | MS.gene28161:CDS |
| TTCTGTTTAACAGGGACAAA+TGG | 0.330082 | 4.4:+9668443 | MS.gene28161:intron |
| TCCTGTCTTCCACTGTTAAT+GGG | 0.334912 | 4.4:+9661946 | MS.gene28161:CDS |
| GCTCTTGGTTTATCAATTGC+TGG | 0.336205 | 4.4:+9659248 | MS.gene28161:CDS |
| TTGTTCGCTGCTCGTGGCCG+TGG | 0.342740 | 4.4:+9659215 | MS.gene28161:CDS |
| CACCACTTATTTGTGCTACT+TGG | 0.343869 | 4.4:+9663273 | MS.gene28161:CDS |
| ATAGTTCTTGCTAGTAGATT+AGG | 0.345177 | 4.4:-9657549 | None:intergenic |
| GAGTTCTTGGCAACACCTTC+GGG | 0.345741 | 4.4:+9657393 | MS.gene28161:CDS |
| AAACTTAAAGAGGAACTAAA+TGG | 0.347516 | 4.4:+9658976 | MS.gene28161:CDS |
| GCCATCAATTGCAGATCATT+TGG | 0.348357 | 4.4:-9663237 | None:intergenic |
| GACAATGCCTTGCAAAATAC+TGG | 0.353277 | 4.4:+9669068 | MS.gene28161:CDS |
| CCGTGCTCTTAGCAGACATT+TGG | 0.355236 | 4.4:-9662212 | None:intergenic |
| CATTATCTCGGCGTCTCCTT+TGG | 0.356220 | 4.4:+9666484 | MS.gene28161:CDS |
| TTCCGTATCAGTGGTCCAAC+AGG | 0.357971 | 4.4:-9659006 | None:intergenic |
| TATTCACAGGTTTGCCTTGA+GGG | 0.360429 | 4.4:+9663610 | MS.gene28161:intron |
| GAAGACAGGAAGACCAGATA+TGG | 0.361242 | 4.4:-9661933 | None:intergenic |
| GATGACCATGGCTCAAACTT+TGG | 0.365532 | 4.4:-9663657 | None:intergenic |
| AGAGTTCTTGGCAACACCTT+CGG | 0.367420 | 4.4:+9657392 | MS.gene28161:CDS |
| GGCTTCCACTTTCCGTGGCA+TGG | 0.373750 | 4.4:+9666905 | MS.gene28161:CDS |
| GCGTTAAGCCAGCTTTCAAT+AGG | 0.380422 | 4.4:-9662862 | None:intergenic |
| TCCAAATGATCTGCAATTGA+TGG | 0.389508 | 4.4:+9663236 | MS.gene28161:CDS |
| AATAAAGCTTAACTGGAATC+TGG | 0.390038 | 4.4:-9666567 | None:intergenic |
| AACAAGGATACCTCTTCAAC+AGG | 0.390312 | 4.4:-9657974 | None:intergenic |
| CCGAGATAATGGAGCTTCTC+CGG | 0.393175 | 4.4:-9666472 | None:intergenic |
| TTCAATTTCATCATTATTCA+AGG | 0.394125 | 4.4:-9666719 | None:intergenic |
| TATAGATCACACTCACTTGC+TGG | 0.394267 | 4.4:-9663022 | None:intergenic |
| TCTACATGGTCTTGCATTCA+AGG | 0.399145 | 4.4:+9668527 | MS.gene28161:CDS |
| CAATCTTGCTGACTTTCATC+TGG | 0.401834 | 4.4:+9667359 | MS.gene28161:CDS |
| CAAAGGAGACGCCGAGATAA+TGG | 0.404698 | 4.4:-9666483 | None:intergenic |
| CGTGGGAAATCAGCTGCTCT+TGG | 0.405723 | 4.4:+9659233 | MS.gene28161:CDS |
| TCTGTTTAACAGGGACAAAT+GGG | 0.420355 | 4.4:+9668444 | MS.gene28161:intron |
| GTGATCTATACTATGTCAAT+CGG | 0.421633 | 4.4:+9663035 | MS.gene28161:CDS |
| CTGCACAGGTCTGCAGAATC+AGG | 0.422621 | 4.4:+9668025 | MS.gene28161:CDS |
| TGGATGACACACAAAATATC+AGG | 0.423019 | 4.4:-9663407 | None:intergenic |
| TGAGCATGTGATAATGTAAC+TGG | 0.428130 | 4.4:-9667987 | None:intergenic |
| TACCAATTTCATCTCACATA+AGG | 0.428327 | 4.4:+9657936 | MS.gene28161:CDS |
| GATACCTCTTCAACAGGAAC+TGG | 0.435352 | 4.4:-9657968 | None:intergenic |
| TTGAAAATGGCGTCAAATCA+AGG | 0.436134 | 4.4:+9656755 | MS.gene28161:CDS |
| GCATCACAGACAGACCATTC+AGG | 0.441433 | 4.4:+9661670 | MS.gene28161:CDS |
| GAATCCCACTCTCAGGCTAC+AGG | 0.446778 | 4.4:+9657836 | MS.gene28161:CDS |
| CGGTGACAAATCCCGTGATC+AGG | 0.453225 | 4.4:+9656801 | MS.gene28161:CDS |
| CATCACCATCGTGTACAAAC+TGG | 0.454198 | 4.4:-9657631 | None:intergenic |
| GTTGATGAAACAAATCAGTT+CGG | 0.459835 | 4.4:+9666742 | MS.gene28161:CDS |
| GAAAATGCATCGGCTTTCTC+AGG | 0.464462 | 4.4:-9657317 | None:intergenic |
| AAATTTGCTCCTTTCTACAT+TGG | 0.466347 | 4.4:+9666544 | MS.gene28161:CDS |
| GCTTCTTCCAAAAGCGAAAG+AGG | 0.467655 | 4.4:-9666519 | None:intergenic |
| CAGCGGTATGCCTTTGAAGA+TGG | 0.471481 | 4.4:+9669032 | MS.gene28161:CDS |
| AGGAAAAGCTTGAACTAAGC+AGG | 0.472967 | 4.4:+9657125 | MS.gene28161:CDS |
| TGTATTCTTACAGCGAATGA+TGG | 0.474059 | 4.4:+9663182 | MS.gene28161:intron |
| CTGTAAGGCATGCGTGGTCA+TGG | 0.476362 | 4.4:+9657895 | MS.gene28161:CDS |
| AGCACAAATAAGTGGTGTGC+TGG | 0.479388 | 4.4:-9663267 | None:intergenic |
| CTATTCACAGGTTTGCCTTG+AGG | 0.480472 | 4.4:+9663609 | MS.gene28161:intron |
| ATTGCTGGTGCTATTGCTGC+CGG | 0.480813 | 4.4:+9659263 | MS.gene28161:CDS |
| ATGCAAAGTTATGAAGGAAC+TGG | 0.481979 | 4.4:+9662144 | MS.gene28161:intron |
| CCAAGAATGCTACTGTTGTA+AGG | 0.489834 | 4.4:+9661633 | MS.gene28161:CDS |
| AAATGAGGAAGATCTGTTCT+GGG | 0.490856 | 4.4:-9666424 | None:intergenic |
| CCAACAGTGAAGTCTTTGCT+TGG | 0.491005 | 4.4:+9661909 | MS.gene28161:CDS |
| CGAGATAATGGAGCTTCTCC+GGG | 0.491889 | 4.4:-9666471 | None:intergenic |
| TGCTAGTACATCTGCGTGAA+AGG | 0.492280 | 4.4:+9666446 | MS.gene28161:CDS |
| TTGTTTGAGTTTATATGCAA+AGG | 0.497050 | 4.4:+9659423 | MS.gene28161:CDS |
| ACTATCAATTGCCTCCTGTA+AGG | 0.497278 | 4.4:+9657880 | MS.gene28161:CDS |
| TGTTCGCTGCTCGTGGCCGT+GGG | 0.501663 | 4.4:+9659216 | MS.gene28161:CDS |
| TGTTTGAGTTTATATGCAAA+GGG | 0.501745 | 4.4:+9659424 | MS.gene28161:CDS |
| ACCCATTAACAGTGGAAGAC+AGG | 0.504081 | 4.4:-9661947 | None:intergenic |
| GCTAAGAGCACGGAGGGTGC+AGG | 0.504530 | 4.4:+9662222 | MS.gene28161:CDS |
| TGATTCTTCGATTCATCCAC+CGG | 0.505238 | 4.4:-9663380 | None:intergenic |
| CTGGGGATCCTATTGAAAGC+TGG | 0.505620 | 4.4:+9662854 | MS.gene28161:CDS |
| TTTCATTGTTTATGATAGGC+GGG | 0.509198 | 4.4:+9657336 | MS.gene28161:CDS |
| CGCAGATGTACTAGCAAATG+AGG | 0.511263 | 4.4:-9666439 | None:intergenic |
| GCTTGTGTGTTACTCTGCAC+AGG | 0.520610 | 4.4:+9668011 | MS.gene28161:CDS |
| CTCAGGATCCAAAAGACCCT+GGG | 0.520939 | 4.4:-9657300 | None:intergenic |
| TCCCTGTTGGACCACTGATA+CGG | 0.521720 | 4.4:+9659004 | MS.gene28161:CDS |
| CCGGAGAAGCTCCATTATCT+CGG | 0.521760 | 4.4:+9666472 | MS.gene28161:CDS |
| TTGTCAGTATAAAATCGAAC+CGG | 0.524708 | 4.4:+9669114 | MS.gene28161:CDS |
| GTATTCCATGCCACGGAAAG+TGG | 0.525307 | 4.4:-9666910 | None:intergenic |
| AGCGAAAGAGGTCCACGGTT+AGG | 0.528419 | 4.4:-9666507 | None:intergenic |
| TCGAACTGAATCCCACTCTC+AGG | 0.529910 | 4.4:+9657829 | MS.gene28161:CDS |
| TACATCTTTCAGCCGTGGCA+TGG | 0.532959 | 4.4:-9668562 | None:intergenic |
| TCAGGACATTTCGCACATTG+AGG | 0.533303 | 4.4:+9668043 | MS.gene28161:CDS |
| TGGCAATGAACTTACCTGAA+TGG | 0.533576 | 4.4:-9661684 | None:intergenic |
| AGGGACAAATGGGGTTGGAA+AGG | 0.535630 | 4.4:+9668454 | MS.gene28161:intron |
| GGAACCTCTTAGTGTTTCTG+TGG | 0.536334 | 4.4:+9668805 | MS.gene28161:CDS |
| GGAATCTGGCCAATGTAGAA+AGG | 0.538089 | 4.4:-9666553 | None:intergenic |
| ATGCCATCCCTAATATTAGC+AGG | 0.538179 | 4.4:+9662908 | MS.gene28161:CDS |
| CTAAATGGAGATTTCCCTGT+TGG | 0.538752 | 4.4:+9658991 | MS.gene28161:CDS |
| TCTTGTCCAAAATTGCATCA+AGG | 0.540070 | 4.4:-9659172 | None:intergenic |
| AGTTACAGAAGCTGCAGAGA+AGG | 0.540274 | 4.4:+9665491 | MS.gene28161:CDS |
| ATAGAGACAGTGGAAGGAGG+AGG | 0.542491 | 4.4:+9657570 | MS.gene28161:CDS |
| CACACATCCTAGTGCAATGA+GGG | 0.543166 | 4.4:+9663774 | MS.gene28161:CDS |
| TAAACCAGTTCCTGTTGAAG+AGG | 0.545632 | 4.4:+9657964 | MS.gene28161:CDS |
| GCTTCTGTAACTGTAATTTG+TGG | 0.547860 | 4.4:-9665480 | None:intergenic |
| GTTTGATGTACTTGAATACA+AGG | 0.548311 | 4.4:+9659446 | MS.gene28161:CDS |
| TCAATTTCATCATTATTCAA+GGG | 0.550319 | 4.4:-9666718 | None:intergenic |
| GCAAGGCTCAAATCAAATCA+AGG | 0.550985 | 4.4:+9657080 | MS.gene28161:CDS |
| TGGATGAAGACCTCAACGAT+GGG | 0.551646 | 4.4:+9668825 | MS.gene28161:CDS |
| TGAGAATGATGTTGACGATA+AGG | 0.553110 | 4.4:+9665449 | MS.gene28161:CDS |
| TTGCTGGTGCTATTGCTGCC+GGG | 0.554680 | 4.4:+9659264 | MS.gene28161:CDS |
| CTTCTGGCTTCCACTTTCCG+TGG | 0.555143 | 4.4:+9666900 | MS.gene28161:CDS |
| ACGGCCACGAGCAGCGAACA+AGG | 0.556001 | 4.4:-9659213 | None:intergenic |
| TGATCTATACTATGTCAATC+GGG | 0.556230 | 4.4:+9663036 | MS.gene28161:CDS |
| CAGCTTTCAATAGGATCCCC+AGG | 0.557416 | 4.4:-9662853 | None:intergenic |
| ACTGTCTGCAGTTATTATGA+AGG | 0.559735 | 4.4:+9665408 | MS.gene28161:intron |
| TCTCAGGATCCAAAAGACCC+TGG | 0.561670 | 4.4:-9657301 | None:intergenic |
| TACCAAGTAGCACAAATAAG+TGG | 0.562485 | 4.4:-9663275 | None:intergenic |
| AAAGACTTCACTGTTGGCAG+TGG | 0.564718 | 4.4:-9661903 | None:intergenic |
| TCTTCATTATATGCTTAGCA+AGG | 0.565411 | 4.4:+9657063 | MS.gene28161:CDS |
| CATCTTCAAAGGCATACCGC+TGG | 0.566759 | 4.4:-9669031 | None:intergenic |
| TTCATTGTTTATGATAGGCG+GGG | 0.569131 | 4.4:+9657337 | MS.gene28161:CDS |
| CCAAGCAAAGACTTCACTGT+TGG | 0.571135 | 4.4:-9661909 | None:intergenic |
| GCTAGATTCTCCATCTTCAA+AGG | 0.571573 | 4.4:-9669042 | None:intergenic |
| TACTGTATAGGACGATATGA+AGG | 0.574051 | 4.4:+9668974 | MS.gene28161:intron |
| TTAACAGGGACAAATGGGGT+TGG | 0.574911 | 4.4:+9668449 | MS.gene28161:intron |
| GAGGTCCACGGTTAGGCCAA+AGG | 0.575632 | 4.4:-9666500 | None:intergenic |
| GAATCCATCAGATATGCTCC+TGG | 0.578441 | 4.4:+9662835 | MS.gene28161:CDS |
| AAGTACAGGTGCGAAAGTGC+AGG | 0.580964 | 4.4:-9667951 | None:intergenic |
| TCCAGACAAAATGAGGAAGA+AGG | 0.581483 | 4.4:+9656708 | None:intergenic |
| CATAAACAATGAAAATGCAT+CGG | 0.584102 | 4.4:-9657327 | None:intergenic |
| CATGGTATTTGATCTCCACA+AGG | 0.584673 | 4.4:-9663682 | None:intergenic |
| TATACTGACAAGACCACCTG+TGG | 0.585384 | 4.4:-9669102 | None:intergenic |
| ATTCACAGGTTTGCCTTGAG+GGG | 0.585666 | 4.4:+9663611 | MS.gene28161:intron |
| AATCCATCAGATATGCTCCT+GGG | 0.586271 | 4.4:+9662836 | MS.gene28161:CDS |
| GAAGTGTTCCACGTTGGACC+AGG | 0.586370 | 4.4:+9659026 | MS.gene28161:CDS |
| GAAGTTACCCTCATTGCACT+AGG | 0.587370 | 4.4:-9663781 | None:intergenic |
| GTTGCCTTGTTCGCTGCTCG+TGG | 0.587390 | 4.4:+9659209 | MS.gene28161:CDS |
| TTAAAGTCTGAAAACGAGCA+TGG | 0.587411 | 4.4:+9669140 | MS.gene28161:CDS |
| ACATCTGCGTGAAAGGCGCC+CGG | 0.587670 | 4.4:+9666453 | MS.gene28161:CDS |
| AACGCCCTGTAGCCTGAGAG+TGG | 0.588671 | 4.4:-9657841 | None:intergenic |
| AAGACAGGAAGACCAGATAT+GGG | 0.589306 | 4.4:-9661932 | None:intergenic |
| AGCAAGAACTATAGAGACAG+TGG | 0.589349 | 4.4:+9657560 | MS.gene28161:CDS |
| CGATGGGGCTAAACAAGTTG+AGG | 0.590660 | 4.4:+9668841 | MS.gene28161:CDS |
| ACCTGATAGAATAACGTGAC+TGG | 0.590808 | 4.4:+9666670 | MS.gene28161:intron |
| TGCCTCCTGTAAGGCATGCG+TGG | 0.591653 | 4.4:+9657889 | MS.gene28161:CDS |
| AGCCATGGTCATCAACCTTG+TGG | 0.591954 | 4.4:+9663667 | MS.gene28161:CDS |
| ATAAAACGATACCTGATCAC+GGG | 0.592447 | 4.4:-9656812 | None:intergenic |
| ATCCCCAGGAGCATATCTGA+TGG | 0.593961 | 4.4:-9662839 | None:intergenic |
| TAATTCACTATCCTTGTGGT+AGG | 0.594044 | 4.4:-9663071 | None:intergenic |
| TTGTAACACCATAAAACAGT+TGG | 0.597418 | 4.4:-9657103 | None:intergenic |
| ACTATAGAGACAGTGGAAGG+AGG | 0.598245 | 4.4:+9657567 | MS.gene28161:CDS |
| AACTGCTCACAAAATTTCCA+TGG | 0.599225 | 4.4:-9663700 | None:intergenic |
| AAAATAATTCACTATCCTTG+TGG | 0.600381 | 4.4:-9663075 | None:intergenic |
| ATGTCTGCTAAGAGCACGGA+GGG | 0.601997 | 4.4:+9662216 | MS.gene28161:CDS |
| AAATTCTACAAATATCTACA+TGG | 0.602639 | 4.4:+9668513 | MS.gene28161:CDS |
| TCATTGTTTATGATAGGCGG+GGG | 0.603523 | 4.4:+9657338 | MS.gene28161:CDS |
| TATAAAACGATACCTGATCA+CGG | 0.604633 | 4.4:-9656813 | None:intergenic |
| GACGAGATATCTGCCCCTCA+AGG | 0.606609 | 4.4:-9663624 | None:intergenic |
| TAACCTGCTAATATTAGGGA+TGG | 0.607113 | 4.4:-9662911 | None:intergenic |
| GGAGCTGAAGAAACTTAAAG+AGG | 0.607451 | 4.4:+9658966 | MS.gene28161:CDS |
| TCATCCACAGAAACACTAAG+AGG | 0.607502 | 4.4:-9668809 | None:intergenic |
| GACTGGCGAGCATTCATGCA+TGG | 0.609058 | 4.4:+9666687 | MS.gene28161:CDS |
| TAGAGACAGTGGAAGGAGGA+GGG | 0.610550 | 4.4:+9657571 | MS.gene28161:CDS |
| CCAAATGTCTGCTAAGAGCA+CGG | 0.613472 | 4.4:+9662212 | MS.gene28161:CDS |
| TAACTTACATCTTTCAGCCG+TGG | 0.614000 | 4.4:-9668567 | None:intergenic |
| CCACACATCCTAGTGCAATG+AGG | 0.617308 | 4.4:+9663773 | MS.gene28161:CDS |
| TGGAATACAAGCTTGCTATG+AGG | 0.617987 | 4.4:+9666925 | MS.gene28161:CDS |
| ATTGATGCTGCAGGACTCAG+AGG | 0.619238 | 4.4:+9658924 | MS.gene28161:intron |
| AATCCCACTCTCAGGCTACA+GGG | 0.620518 | 4.4:+9657837 | MS.gene28161:CDS |
| ATGCGAGCTCCTGAAAGTGA+AGG | 0.624310 | 4.4:-9663739 | None:intergenic |
| CAGTTTGTACACGATGGTGA+TGG | 0.626116 | 4.4:+9657632 | MS.gene28161:CDS |
| CAAGCTTGTATTCCATGCCA+CGG | 0.626773 | 4.4:-9666917 | None:intergenic |
| AGACAAAATGAGGAAGAAGG+TGG | 0.627687 | 4.4:+9656711 | None:intergenic |
| CGTCTCCTTTGGCCTAACCG+TGG | 0.629616 | 4.4:+9666495 | MS.gene28161:CDS |
| GTGGATGAAGACCTCAACGA+TGG | 0.630682 | 4.4:+9668824 | MS.gene28161:CDS |
| GCTATCCAAAGTTTGAGCCA+TGG | 0.630986 | 4.4:+9663652 | MS.gene28161:CDS |
| CCTCATTGCACTAGGATGTG+TGG | 0.631104 | 4.4:-9663773 | None:intergenic |
| TATTTGTATGACAGGACCGG+TGG | 0.632393 | 4.4:+9663364 | MS.gene28161:intron |
| GTGGAACACTTCCGTATCAG+TGG | 0.634428 | 4.4:-9659015 | None:intergenic |
| TTGATGCTGCAGGACTCAGA+GGG | 0.636185 | 4.4:+9658925 | MS.gene28161:intron |
| ACGCCCTGTAGCCTGAGAGT+GGG | 0.637356 | 4.4:-9657840 | None:intergenic |
| CTCCACAAGGTTGATGACCA+TGG | 0.637837 | 4.4:-9663669 | None:intergenic |
| CTTGTTTAGCCCCATCGTTG+AGG | 0.638898 | 4.4:-9668835 | None:intergenic |
| CTGTTTAACAGGGACAAATG+GGG | 0.641129 | 4.4:+9668445 | MS.gene28161:intron |
| ATTCAGTTCGAAATCTGTCG+TGG | 0.643656 | 4.4:-9657817 | None:intergenic |
| TTTGCATATAAACTCAAACA+AGG | 0.644235 | 4.4:-9659421 | None:intergenic |
| CTTGTGGAGATCAAATACCA+TGG | 0.649950 | 4.4:+9663683 | MS.gene28161:CDS |
| AGATCGAATCAACCATGCCA+CGG | 0.650410 | 4.4:+9668550 | MS.gene28161:CDS |
| TCACTCCTGAATTGCTCCAG+CGG | 0.651035 | 4.4:+9669015 | MS.gene28161:CDS |
| CAAGAGCAGCTGATTTCCCA+CGG | 0.651139 | 4.4:-9659232 | None:intergenic |
| AGAACTATAGAGACAGTGGA+AGG | 0.654646 | 4.4:+9657564 | MS.gene28161:CDS |
| GTCACCGATAATGACAAACA+TGG | 0.659550 | 4.4:-9656785 | None:intergenic |
| TCTAGTCGCTTCATATCATG+TGG | 0.661842 | 4.4:-9667324 | None:intergenic |
| TCCGTATCAGTGGTCCAACA+GGG | 0.663742 | 4.4:-9659005 | None:intergenic |
| ATCCATCAGATATGCTCCTG+GGG | 0.663879 | 4.4:+9662837 | MS.gene28161:CDS |
| ACATGATATGAAGCGACTAG+AGG | 0.666290 | 4.4:+9667326 | MS.gene28161:CDS |
| AATGCCTTGCAAAATACTGG+TGG | 0.667743 | 4.4:+9669071 | MS.gene28161:CDS |
| CCAAAAGCGAAAGAGGTCCA+CGG | 0.671055 | 4.4:-9666512 | None:intergenic |
| CTGAAGAACACACATCCCGA+AGG | 0.674615 | 4.4:-9657408 | None:intergenic |
| AGATCACACTCACTTGCTGG+AGG | 0.685877 | 4.4:-9663019 | None:intergenic |
| ATACTGACAAGACCACCTGT+GGG | 0.694097 | 4.4:-9669101 | None:intergenic |
| GCTAACCAGTTTGTACACGA+TGG | 0.697246 | 4.4:+9657626 | MS.gene28161:CDS |
| GATACGGAAGTGTTCCACGT+TGG | 0.701379 | 4.4:+9659020 | MS.gene28161:CDS |
| GGATGAAGACCTCAACGATG+GGG | 0.701949 | 4.4:+9668826 | MS.gene28161:CDS |
| TAATCCTACCTGGTCCAACG+TGG | 0.704729 | 4.4:-9659034 | None:intergenic |
| CCAGTCATACCCATTAACAG+TGG | 0.707802 | 4.4:-9661955 | None:intergenic |
| GACCACGCATGCCTTACAGG+AGG | 0.710844 | 4.4:-9657891 | None:intergenic |
| AAGTCTGAAAACGAGCATGG+AGG | 0.737070 | 4.4:+9669143 | MS.gene28161:CDS |
| AATGTCTGCTAAGAGCACGG+AGG | 0.777641 | 4.4:+9662215 | MS.gene28161:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 9656718 | 9669241 | 9656718 | ID=MS.gene28161 |
| chr4.4 | mRNA | 9656718 | 9669241 | 9656718 | ID=MS.gene28161.t1;Parent=MS.gene28161 |
| chr4.4 | exon | 9656718 | 9656822 | 9656718 | ID=MS.gene28161.t1.exon1;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9656718 | 9656822 | 9656718 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9657055 | 9657146 | 9657055 | ID=MS.gene28161.t1.exon2;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9657055 | 9657146 | 9657055 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9657259 | 9657430 | 9657259 | ID=MS.gene28161.t1.exon3;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9657259 | 9657430 | 9657259 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9657531 | 9657653 | 9657531 | ID=MS.gene28161.t1.exon4;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9657531 | 9657653 | 9657531 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9657812 | 9657985 | 9657812 | ID=MS.gene28161.t1.exon5;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9657812 | 9657985 | 9657812 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9658937 | 9659047 | 9658937 | ID=MS.gene28161.t1.exon6;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9658937 | 9659047 | 9658937 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9659152 | 9659285 | 9659152 | ID=MS.gene28161.t1.exon7;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9659152 | 9659285 | 9659152 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9659374 | 9659467 | 9659374 | ID=MS.gene28161.t1.exon8;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9659374 | 9659467 | 9659374 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9661596 | 9661691 | 9661596 | ID=MS.gene28161.t1.exon9;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9661596 | 9661691 | 9661596 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9661831 | 9661967 | 9661831 | ID=MS.gene28161.t1.exon10;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9661831 | 9661967 | 9661831 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9662152 | 9662243 | 9662152 | ID=MS.gene28161.t1.exon11;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9662152 | 9662243 | 9662152 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9662806 | 9662929 | 9662806 | ID=MS.gene28161.t1.exon12;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9662806 | 9662929 | 9662806 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9663015 | 9663102 | 9663015 | ID=MS.gene28161.t1.exon13;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9663015 | 9663102 | 9663015 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9663195 | 9663294 | 9663195 | ID=MS.gene28161.t1.exon14;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9663195 | 9663294 | 9663195 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9663378 | 9663430 | 9663378 | ID=MS.gene28161.t1.exon15;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9663378 | 9663430 | 9663378 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9663619 | 9663795 | 9663619 | ID=MS.gene28161.t1.exon16;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9663619 | 9663795 | 9663619 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9663890 | 9663927 | 9663890 | ID=MS.gene28161.t1.exon17;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9663890 | 9663927 | 9663890 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9665419 | 9665512 | 9665419 | ID=MS.gene28161.t1.exon18;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9665419 | 9665512 | 9665419 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9666397 | 9666576 | 9666397 | ID=MS.gene28161.t1.exon19;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9666397 | 9666576 | 9666397 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9666679 | 9666787 | 9666679 | ID=MS.gene28161.t1.exon20;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9666679 | 9666787 | 9666679 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9666877 | 9666946 | 9666877 | ID=MS.gene28161.t1.exon21;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9666877 | 9666946 | 9666877 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9667233 | 9667380 | 9667233 | ID=MS.gene28161.t1.exon22;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9667233 | 9667380 | 9667233 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9667936 | 9668064 | 9667936 | ID=MS.gene28161.t1.exon23;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9667936 | 9668064 | 9667936 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9668456 | 9668581 | 9668456 | ID=MS.gene28161.t1.exon24;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9668456 | 9668581 | 9668456 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9668797 | 9668862 | 9668797 | ID=MS.gene28161.t1.exon25;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9668797 | 9668862 | 9668797 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| chr4.4 | exon | 9668984 | 9669241 | 9668984 | ID=MS.gene28161.t1.exon26;Parent=MS.gene28161.t1 |
| chr4.4 | CDS | 9668984 | 9669241 | 9668984 | ID=cds.MS.gene28161.t1;Parent=MS.gene28161.t1 |
| Gene Sequence |
| Protein sequence |
