Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015460.t1 | XP_003611259.1 | 98.1 | 320 | 5 | 1 | 1 | 319 | 1 | 320 | 1.60E-157 | 565.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015460.t1 | Q42374 | 66.6 | 320 | 96 | 4 | 1 | 319 | 1 | 310 | 6.5e-99 | 362.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015460.t1 | G7KG89 | 98.1 | 320 | 5 | 1 | 1 | 319 | 1 | 320 | 1.2e-157 | 565.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene052542 | MS.gene015460 | PPI |
| MS.gene65117 | MS.gene015460 | PPI |
| MS.gene015460 | MS.gene01347 | PPI |
| MS.gene66057 | MS.gene015460 | PPI |
| MS.gene33736 | MS.gene015460 | PPI |
| MS.gene01343 | MS.gene015460 | PPI |
| MS.gene047508 | MS.gene015460 | PPI |
| MS.gene78212 | MS.gene015460 | PPI |
| MS.gene066610 | MS.gene015460 | PPI |
| MS.gene002374 | MS.gene015460 | PPI |
| MS.gene99067 | MS.gene015460 | PPI |
| MS.gene99119 | MS.gene015460 | PPI |
| MS.gene50283 | MS.gene015460 | PPI |
| MS.gene32192 | MS.gene015460 | PPI |
| MS.gene77217 | MS.gene015460 | PPI |
| MS.gene01756 | MS.gene015460 | PPI |
| MS.gene035217 | MS.gene015460 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015460.t1 | MTR_5g012010 | 98.438 | 320 | 4 | 1 | 1 | 319 | 1 | 320 | 0.0 | 637 |
| MS.gene015460.t1 | MTR_2g012270 | 47.619 | 315 | 147 | 3 | 1 | 315 | 1 | 297 | 1.89e-105 | 310 |
| MS.gene015460.t1 | MTR_4g090900 | 50.000 | 296 | 129 | 4 | 1 | 295 | 1 | 278 | 3.30e-99 | 295 |
| MS.gene015460.t1 | MTR_1g056550 | 42.617 | 298 | 155 | 3 | 1 | 295 | 1 | 285 | 3.61e-85 | 259 |
| MS.gene015460.t1 | MTR_1g056560 | 48.583 | 247 | 126 | 1 | 49 | 295 | 44 | 289 | 1.12e-83 | 256 |
| MS.gene015460.t1 | MTR_2g088700 | 44.025 | 318 | 162 | 5 | 1 | 316 | 1 | 304 | 2.53e-80 | 246 |
| MS.gene015460.t1 | MTR_5g020670 | 46.823 | 299 | 131 | 7 | 1 | 294 | 1 | 276 | 2.68e-77 | 238 |
| MS.gene015460.t1 | MTR_7g106420 | 45.306 | 245 | 133 | 1 | 53 | 297 | 31 | 274 | 2.96e-76 | 236 |
| MS.gene015460.t1 | MTR_2g088700 | 44.781 | 297 | 148 | 5 | 1 | 295 | 1 | 283 | 5.91e-76 | 235 |
| MS.gene015460.t1 | MTR_6g028140 | 41.077 | 297 | 158 | 3 | 1 | 297 | 1 | 280 | 9.15e-73 | 227 |
| MS.gene015460.t1 | MTR_8g073900 | 36.813 | 182 | 75 | 5 | 1 | 178 | 1 | 146 | 2.05e-22 | 91.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015460.t1 | AT1G08560 | 65.000 | 320 | 101 | 5 | 1 | 319 | 1 | 310 | 1.38e-132 | 379 |
| MS.gene015460.t1 | AT1G61290 | 47.923 | 313 | 141 | 4 | 1 | 311 | 1 | 293 | 2.31e-102 | 302 |
| MS.gene015460.t1 | AT1G61290 | 47.923 | 313 | 141 | 4 | 1 | 311 | 1 | 293 | 2.31e-102 | 302 |
| MS.gene015460.t1 | AT4G03330 | 47.619 | 315 | 150 | 3 | 1 | 315 | 1 | 300 | 1.41e-93 | 280 |
| MS.gene015460.t1 | AT1G11250 | 47.651 | 298 | 127 | 5 | 1 | 295 | 1 | 272 | 2.94e-90 | 271 |
| MS.gene015460.t1 | AT3G11820 | 50.192 | 261 | 129 | 1 | 35 | 295 | 29 | 288 | 4.06e-87 | 265 |
| MS.gene015460.t1 | AT3G11820 | 52.227 | 247 | 117 | 1 | 49 | 295 | 12 | 257 | 7.20e-87 | 263 |
| MS.gene015460.t1 | AT3G52400 | 43.289 | 298 | 159 | 3 | 1 | 297 | 1 | 289 | 2.10e-79 | 245 |
| MS.gene015460.t1 | AT2G18260 | 38.095 | 315 | 184 | 4 | 1 | 315 | 1 | 304 | 8.63e-70 | 219 |
| MS.gene015460.t1 | AT2G18260 | 38.095 | 315 | 184 | 4 | 1 | 315 | 1 | 304 | 8.63e-70 | 219 |
| MS.gene015460.t1 | AT3G03800 | 42.642 | 265 | 151 | 1 | 53 | 317 | 38 | 301 | 8.05e-68 | 214 |
| MS.gene015460.t1 | AT3G03800 | 42.642 | 265 | 151 | 1 | 53 | 317 | 38 | 301 | 8.05e-68 | 214 |
| MS.gene015460.t1 | AT5G08080 | 43.729 | 295 | 149 | 4 | 1 | 295 | 1 | 278 | 1.01e-66 | 211 |
| MS.gene015460.t1 | AT5G08080 | 43.729 | 295 | 149 | 4 | 1 | 295 | 1 | 278 | 1.01e-66 | 211 |
| MS.gene015460.t1 | AT5G08080 | 43.434 | 297 | 158 | 6 | 1 | 295 | 1 | 289 | 1.22e-65 | 209 |
| MS.gene015460.t1 | AT5G08080 | 39.171 | 217 | 115 | 4 | 1 | 217 | 1 | 200 | 5.38e-32 | 119 |
| MS.gene015460.t1 | AT5G08080 | 39.171 | 217 | 115 | 4 | 1 | 217 | 1 | 200 | 5.38e-32 | 119 |
Find 46 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGATTGAGAAGAGTTTAT+TGG | 0.159342 | 5.4:-6214275 | MS.gene015460:CDS |
| GGAGATGATGAAAGTTTCTT+AGG | 0.183110 | 5.4:-6214376 | MS.gene015460:CDS |
| GGAGCTTATGATGGAGTTTC+AGG | 0.211805 | 5.4:-6214763 | MS.gene015460:intron |
| TGAAGAAAGCAAAATGTATT+AGG | 0.253459 | 5.4:-6214911 | MS.gene015460:CDS |
| GCCATTAGTAACAGCAATTC+TGG | 0.268624 | 5.4:+6214803 | None:intergenic |
| AACTGATATGAATCTGTTCT+TGG | 0.361438 | 5.4:-6215084 | MS.gene015460:CDS |
| AAGATCTTCACTGACCCATT+TGG | 0.382232 | 5.4:-6215108 | MS.gene015460:CDS |
| AGAGAAAGTAAGAACAGAAA+TGG | 0.386214 | 5.4:-6215054 | MS.gene015460:CDS |
| ACATGGAAGAGGAAAGGTTC+TGG | 0.392183 | 5.4:-6214338 | MS.gene015460:CDS |
| TAGGACTCAACTTGAAGAAA+TGG | 0.395349 | 5.4:-6214892 | MS.gene015460:CDS |
| ACCAGAATTGCTGTTACTAA+TGG | 0.398063 | 5.4:-6214804 | MS.gene015460:CDS |
| ATTCTGGTTCTATAAATTGC+TGG | 0.415838 | 5.4:+6214819 | None:intergenic |
| GATTCATATCAGTTTCCAAA+TGG | 0.439557 | 5.4:+6215093 | None:intergenic |
| GATGAAGTTGATTTAGAAGC+TGG | 0.444831 | 5.4:-6215161 | MS.gene015460:CDS |
| GCAGGAAGTGGATGTGCATT+GGG | 0.446829 | 5.4:-6214102 | MS.gene015460:CDS |
| CTGCTGAAACTCACTACAAT+GGG | 0.453019 | 5.4:+6214040 | None:intergenic |
| GCTGCTAATAGAAGACTCTC+TGG | 0.458709 | 5.4:-6214858 | MS.gene015460:CDS |
| ATTGAGAAGATAATTTCAAA+TGG | 0.459387 | 5.4:-6214397 | MS.gene015460:CDS |
| GCTTCTCATTATGTTAAAGA+TGG | 0.464968 | 5.4:-6214166 | MS.gene015460:CDS |
| GTATATTACAACAGGGACTT+AGG | 0.467475 | 5.4:-6214501 | MS.gene015460:intron |
| GAGATGATGAAAGTTTCTTA+GGG | 0.469669 | 5.4:-6214375 | MS.gene015460:CDS |
| ATTCATATCAGTTTCCAAAT+GGG | 0.473520 | 5.4:+6215094 | None:intergenic |
| AGCAGGAAGTGGATGTGCAT+TGG | 0.476019 | 5.4:-6214103 | MS.gene015460:CDS |
| AGAAGGTATTATACAGTGAC+AGG | 0.477649 | 5.4:-6214442 | MS.gene015460:CDS |
| ATCAGAAAAGTAGCAGGAAG+TGG | 0.480407 | 5.4:-6214114 | MS.gene015460:CDS |
| AACAGTATCAGAAAAGTAGC+AGG | 0.504319 | 5.4:-6214120 | MS.gene015460:CDS |
| TATGAATCTGTTCTTGGAAG+AGG | 0.513466 | 5.4:-6215078 | MS.gene015460:CDS |
| GAAACTCAAGGAGCTTATGA+TGG | 0.521896 | 5.4:-6214772 | MS.gene015460:CDS |
| GATGGTACAAAGAATCTTCA+CGG | 0.525784 | 5.4:-6214148 | MS.gene015460:CDS |
| GAGAAAGTAAGAACAGAAAT+GGG | 0.532673 | 5.4:-6215053 | MS.gene015460:CDS |
| AGTGAATACAAAGAAACAGT+AGG | 0.536819 | 5.4:-6214466 | MS.gene015460:CDS |
| TGATGAAAGTTTCTTAGGGA+AGG | 0.540453 | 5.4:-6214371 | MS.gene015460:CDS |
| AGACTCTCTGGACTTAAAGA+TGG | 0.542560 | 5.4:-6214846 | MS.gene015460:CDS |
| GCAATACAAGAACATGGAAG+AGG | 0.566682 | 5.4:-6214349 | MS.gene015460:CDS |
| ACTGCTGAAACTCACTACAA+TGG | 0.567938 | 5.4:+6214039 | None:intergenic |
| TGAAGCTTTGAAAGCACTGA+GGG | 0.568866 | 5.4:-6214964 | MS.gene015460:CDS |
| GTGAATACAAAGAAACAGTA+GGG | 0.579417 | 5.4:-6214465 | MS.gene015460:CDS |
| ACAAAGAAACAGTAGGGAGA+AGG | 0.588838 | 5.4:-6214459 | MS.gene015460:CDS |
| TGGCTTGCGTAAGAAACTCA+AGG | 0.596215 | 5.4:-6214784 | MS.gene015460:CDS |
| ACAAGAACATGGAAGAGGAA+AGG | 0.606969 | 5.4:-6214344 | MS.gene015460:CDS |
| GGGAAGGCAATACAAGAACA+TGG | 0.609551 | 5.4:-6214355 | MS.gene015460:CDS |
| AGGAAAGGTTCTGGAAACTG+TGG | 0.665351 | 5.4:-6214329 | MS.gene015460:CDS |
| GAAGGTATTATACAGTGACA+GGG | 0.682716 | 5.4:-6214441 | MS.gene015460:CDS |
| CTGAAGCTTTGAAAGCACTG+AGG | 0.693842 | 5.4:-6214965 | MS.gene015460:CDS |
| GCTGTTATGGTTGAAGCACA+AGG | 0.705464 | 5.4:-6214226 | MS.gene015460:CDS |
| AAGGTATTATACAGTGACAG+GGG | 0.727159 | 5.4:-6214440 | MS.gene015460:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATTTATGTATCAATTTTA+AGG | - | chr5.4:6214583-6214602 | MS.gene015460:intron | 15.0% |
| !! | ATTGAGAAGATAATTTCAAA+TGG | - | chr5.4:6214860-6214879 | MS.gene015460:CDS | 20.0% |
| !!! | TCATCAAATATTTTTGGATA+TGG | - | chr5.4:6215009-6215028 | MS.gene015460:CDS | 20.0% |
| !!! | TTTTTTTAGATCAACATAGT+TGG | + | chr5.4:6214064-6214083 | None:intergenic | 20.0% |
| ! | AAACCTTGTATATTACAACA+GGG | - | chr5.4:6214749-6214768 | MS.gene015460:intron | 25.0% |
| ! | ATATTTGTGTAAGAAACTCA+TGG | - | chr5.4:6214618-6214637 | MS.gene015460:intron | 25.0% |
| ! | ATTCATATCAGTTTCCAAAT+GGG | + | chr5.4:6214166-6214185 | None:intergenic | 25.0% |
| ! | TGAAGAAAGCAAAATGTATT+AGG | - | chr5.4:6214346-6214365 | MS.gene015460:CDS | 25.0% |
| !! | GATTATACTTTTGCTTATTC+TGG | - | chr5.4:6215177-6215196 | MS.gene015460:CDS | 25.0% |
| !! | GGAACTTCATCAAATATTTT+TGG | - | chr5.4:6215003-6215022 | MS.gene015460:CDS | 25.0% |
| !!! | ACAATGATGCTTTTTAGAAA+AGG | + | chr5.4:6214662-6214681 | None:intergenic | 25.0% |
| AACTGATATGAATCTGTTCT+TGG | - | chr5.4:6214173-6214192 | MS.gene015460:CDS | 30.0% | |
| AGAGAAAGTAAGAACAGAAA+TGG | - | chr5.4:6214203-6214222 | MS.gene015460:CDS | 30.0% | |
| AGAGATTGAGAAGAGTTTAT+TGG | - | chr5.4:6214982-6215001 | MS.gene015460:CDS | 30.0% | |
| AGTGAATACAAAGAAACAGT+AGG | - | chr5.4:6214791-6214810 | MS.gene015460:CDS | 30.0% | |
| GAAACCTTGTATATTACAAC+AGG | - | chr5.4:6214748-6214767 | MS.gene015460:intron | 30.0% | |
| GAGAAAGTAAGAACAGAAAT+GGG | - | chr5.4:6214204-6214223 | MS.gene015460:CDS | 30.0% | |
| GAGATGATGAAAGTTTCTTA+GGG | - | chr5.4:6214882-6214901 | MS.gene015460:CDS | 30.0% | |
| GATTCATATCAGTTTCCAAA+TGG | + | chr5.4:6214167-6214186 | None:intergenic | 30.0% | |
| GCTTCTCATTATGTTAAAGA+TGG | - | chr5.4:6215091-6215110 | MS.gene015460:CDS | 30.0% | |
| GTGAATACAAAGAAACAGTA+GGG | - | chr5.4:6214792-6214811 | MS.gene015460:CDS | 30.0% | |
| !! | ATTCTGGTTCTATAAATTGC+TGG | + | chr5.4:6214441-6214460 | None:intergenic | 30.0% |
| !! | ATTTTTGGATATGGCTGTTA+TGG | - | chr5.4:6215018-6215037 | MS.gene015460:CDS | 30.0% |
| !!! | TCTTCTGGTTTTTTTCTTTC+TGG | - | chr5.4:6214537-6214556 | MS.gene015460:intron | 30.0% |
| AACAGTATCAGAAAAGTAGC+AGG | - | chr5.4:6215137-6215156 | MS.gene015460:CDS | 35.0% | |
| AACCAGAAGAGAAATTGACA+AGG | + | chr5.4:6214527-6214546 | None:intergenic | 35.0% | |
| ACCAGAAGAGAAATTGACAA+GGG | + | chr5.4:6214526-6214545 | None:intergenic | 35.0% | |
| AGTCCCTGTTGTAATATACA+AGG | + | chr5.4:6214755-6214774 | None:intergenic | 35.0% | |
| GATGGTACAAAGAATCTTCA+CGG | - | chr5.4:6215109-6215128 | MS.gene015460:CDS | 35.0% | |
| GGAGATGATGAAAGTTTCTT+AGG | - | chr5.4:6214881-6214900 | MS.gene015460:CDS | 35.0% | |
| GTATATTACAACAGGGACTT+AGG | - | chr5.4:6214756-6214775 | MS.gene015460:intron | 35.0% | |
| TAGGACTCAACTTGAAGAAA+TGG | - | chr5.4:6214365-6214384 | MS.gene015460:CDS | 35.0% | |
| TATGAATCTGTTCTTGGAAG+AGG | - | chr5.4:6214179-6214198 | MS.gene015460:CDS | 35.0% | |
| TGAAGCACAAGGTGAAAAAA+TGG | - | chr5.4:6215042-6215061 | MS.gene015460:CDS | 35.0% | |
| TGATGAAAGTTTCTTAGGGA+AGG | - | chr5.4:6214886-6214905 | MS.gene015460:CDS | 35.0% | |
| ! | AAGGTATTATACAGTGACAG+GGG | - | chr5.4:6214817-6214836 | MS.gene015460:CDS | 35.0% |
| ! | ACCAGAATTGCTGTTACTAA+TGG | - | chr5.4:6214453-6214472 | MS.gene015460:CDS | 35.0% |
| ! | GATGAAGTTGATTTAGAAGC+TGG | - | chr5.4:6214096-6214115 | MS.gene015460:CDS | 35.0% |
| !! | AGAAGGTATTATACAGTGAC+AGG | - | chr5.4:6214815-6214834 | MS.gene015460:CDS | 35.0% |
| !! | GAAGGTATTATACAGTGACA+GGG | - | chr5.4:6214816-6214835 | MS.gene015460:CDS | 35.0% |
| AAGATCTTCACTGACCCATT+TGG | - | chr5.4:6214149-6214168 | MS.gene015460:CDS | 40.0% | |
| ACAAAGAAACAGTAGGGAGA+AGG | - | chr5.4:6214798-6214817 | MS.gene015460:CDS | 40.0% | |
| ACAAGAACATGGAAGAGGAA+AGG | - | chr5.4:6214913-6214932 | MS.gene015460:CDS | 40.0% | |
| ACCCTTGTCAATTTCTCTTC+TGG | - | chr5.4:6214522-6214541 | MS.gene015460:intron | 40.0% | |
| ACTGCTGAAACTCACTACAA+TGG | + | chr5.4:6215221-6215240 | None:intergenic | 40.0% | |
| AGACTCTCTGGACTTAAAGA+TGG | - | chr5.4:6214411-6214430 | MS.gene015460:CDS | 40.0% | |
| ATCAGAAAAGTAGCAGGAAG+TGG | - | chr5.4:6215143-6215162 | MS.gene015460:CDS | 40.0% | |
| CTGCTGAAACTCACTACAAT+GGG | + | chr5.4:6215220-6215239 | None:intergenic | 40.0% | |
| GAAACTCAAGGAGCTTATGA+TGG | - | chr5.4:6214485-6214504 | MS.gene015460:CDS | 40.0% | |
| GCAATACAAGAACATGGAAG+AGG | - | chr5.4:6214908-6214927 | MS.gene015460:CDS | 40.0% | |
| GCCATTAGTAACAGCAATTC+TGG | + | chr5.4:6214457-6214476 | None:intergenic | 40.0% | |
| !! | GAAGCTGGTCTTCAAAAACA+AGG | - | chr5.4:6214111-6214130 | MS.gene015460:CDS | 40.0% |
| !! | TGAAGCTTTGAAAGCACTGA+GGG | - | chr5.4:6214293-6214312 | MS.gene015460:CDS | 40.0% |
| ACATGGAAGAGGAAAGGTTC+TGG | - | chr5.4:6214919-6214938 | MS.gene015460:CDS | 45.0% | |
| GCTGCTAATAGAAGACTCTC+TGG | - | chr5.4:6214399-6214418 | MS.gene015460:CDS | 45.0% | |
| GCTGTTATGGTTGAAGCACA+AGG | - | chr5.4:6215031-6215050 | MS.gene015460:CDS | 45.0% | |
| GGGAAGGCAATACAAGAACA+TGG | - | chr5.4:6214902-6214921 | MS.gene015460:CDS | 45.0% | |
| ! | GGAGCTTATGATGGAGTTTC+AGG | - | chr5.4:6214494-6214513 | MS.gene015460:intron | 45.0% |
| ! | TGGCTTGCGTAAGAAACTCA+AGG | - | chr5.4:6214473-6214492 | MS.gene015460:CDS | 45.0% |
| !! | AGGAAAGGTTCTGGAAACTG+TGG | - | chr5.4:6214928-6214947 | MS.gene015460:CDS | 45.0% |
| !! | CTGAAGCTTTGAAAGCACTG+AGG | - | chr5.4:6214292-6214311 | MS.gene015460:CDS | 45.0% |
| !! | GCTGGTCTTCAAAAACAAGG+TGG | - | chr5.4:6214114-6214133 | MS.gene015460:CDS | 45.0% |
| AGCAGGAAGTGGATGTGCAT+TGG | - | chr5.4:6215154-6215173 | MS.gene015460:CDS | 50.0% | |
| GCAGGAAGTGGATGTGCATT+GGG | - | chr5.4:6215155-6215174 | MS.gene015460:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 6214033 | 6215246 | 6214033 | ID=MS.gene015460 |
| chr5.4 | mRNA | 6214033 | 6215246 | 6214033 | ID=MS.gene015460.t1;Parent=MS.gene015460 |
| chr5.4 | exon | 6214764 | 6215246 | 6214764 | ID=MS.gene015460.t1.exon1;Parent=MS.gene015460.t1 |
| chr5.4 | CDS | 6214764 | 6215246 | 6214764 | ID=cds.MS.gene015460.t1;Parent=MS.gene015460.t1 |
| chr5.4 | exon | 6214033 | 6214509 | 6214033 | ID=MS.gene015460.t1.exon2;Parent=MS.gene015460.t1 |
| chr5.4 | CDS | 6214033 | 6214509 | 6214033 | ID=cds.MS.gene015460.t1;Parent=MS.gene015460.t1 |
| Gene Sequence |
| Protein sequence |
