Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002374.t1 | XP_024632050.1 | 99.7 | 304 | 1 | 0 | 1 | 304 | 1 | 304 | 2.10E-151 | 545 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002374.t1 | O64791 | 80.9 | 282 | 54 | 0 | 1 | 282 | 1 | 282 | 1.2e-123 | 444.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002374.t1 | G7ILD5 | 99.7 | 304 | 1 | 0 | 1 | 304 | 1 | 304 | 1.5e-151 | 545.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene064565 | MS.gene002374 | PPI |
| MS.gene033077 | MS.gene002374 | PPI |
| MS.gene21144 | MS.gene002374 | PPI |
| MS.gene002374 | MS.gene063476 | PPI |
| MS.gene002374 | MS.gene55383 | PPI |
| MS.gene002374 | MS.gene015460 | PPI |
| MS.gene035781 | MS.gene002374 | PPI |
| MS.gene002374 | MS.gene99692 | PPI |
| MS.gene002374 | MS.gene044431 | PPI |
| MS.gene002374 | MS.gene69311 | PPI |
| MS.gene002374 | MS.gene70948 | PPI |
| MS.gene73141 | MS.gene002374 | PPI |
| MS.gene002374 | MS.gene09947 | PPI |
| MS.gene002374 | MS.gene025786 | PPI |
| MS.gene002374 | MS.gene53170 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002374.t1 | MTR_2g012270 | 99.671 | 304 | 1 | 0 | 1 | 304 | 1 | 304 | 0.0 | 622 |
| MS.gene002374.t1 | MTR_4g090900 | 90.000 | 280 | 27 | 1 | 1 | 279 | 1 | 280 | 0.0 | 525 |
| MS.gene002374.t1 | MTR_1g056550 | 58.042 | 286 | 110 | 2 | 1 | 277 | 1 | 285 | 6.13e-122 | 352 |
| MS.gene002374.t1 | MTR_1g056560 | 56.701 | 291 | 114 | 1 | 1 | 279 | 1 | 291 | 4.61e-119 | 345 |
| MS.gene002374.t1 | MTR_7g106420 | 57.490 | 247 | 105 | 0 | 33 | 279 | 28 | 274 | 3.20e-105 | 309 |
| MS.gene002374.t1 | MTR_5g012010 | 49.324 | 296 | 131 | 4 | 1 | 277 | 1 | 296 | 7.76e-99 | 293 |
| MS.gene002374.t1 | MTR_2g088700 | 44.444 | 306 | 161 | 3 | 1 | 300 | 1 | 303 | 2.52e-87 | 264 |
| MS.gene002374.t1 | MTR_6g028140 | 46.953 | 279 | 145 | 2 | 1 | 277 | 1 | 278 | 2.24e-86 | 261 |
| MS.gene002374.t1 | MTR_2g088700 | 45.936 | 283 | 147 | 1 | 1 | 277 | 1 | 283 | 6.28e-86 | 260 |
| MS.gene002374.t1 | MTR_5g020670 | 43.206 | 287 | 145 | 4 | 1 | 278 | 1 | 278 | 3.75e-70 | 219 |
| MS.gene002374.t1 | MTR_8g073900 | 39.286 | 168 | 72 | 3 | 1 | 160 | 1 | 146 | 2.35e-23 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002374.t1 | AT1G61290 | 77.961 | 304 | 63 | 1 | 1 | 304 | 1 | 300 | 6.06e-180 | 498 |
| MS.gene002374.t1 | AT1G61290 | 77.961 | 304 | 63 | 1 | 1 | 304 | 1 | 300 | 6.06e-180 | 498 |
| MS.gene002374.t1 | AT1G11250 | 79.211 | 279 | 53 | 1 | 1 | 279 | 1 | 274 | 4.53e-166 | 463 |
| MS.gene002374.t1 | AT4G03330 | 68.543 | 302 | 92 | 1 | 1 | 299 | 1 | 302 | 1.16e-146 | 414 |
| MS.gene002374.t1 | AT3G11820 | 65.992 | 247 | 84 | 0 | 31 | 277 | 11 | 257 | 9.78e-120 | 347 |
| MS.gene002374.t1 | AT3G11820 | 63.566 | 258 | 94 | 0 | 20 | 277 | 31 | 288 | 2.19e-119 | 347 |
| MS.gene002374.t1 | AT3G52400 | 54.671 | 289 | 121 | 2 | 1 | 279 | 1 | 289 | 3.56e-105 | 310 |
| MS.gene002374.t1 | AT3G03800 | 47.872 | 282 | 139 | 2 | 1 | 277 | 1 | 279 | 2.49e-88 | 266 |
| MS.gene002374.t1 | AT3G03800 | 47.872 | 282 | 139 | 2 | 1 | 277 | 1 | 279 | 2.49e-88 | 266 |
| MS.gene002374.t1 | AT1G08560 | 46.048 | 291 | 138 | 4 | 1 | 277 | 1 | 286 | 3.17e-85 | 258 |
| MS.gene002374.t1 | AT5G08080 | 49.643 | 280 | 136 | 2 | 1 | 277 | 1 | 278 | 1.14e-80 | 246 |
| MS.gene002374.t1 | AT5G08080 | 49.643 | 280 | 136 | 2 | 1 | 277 | 1 | 278 | 1.14e-80 | 246 |
| MS.gene002374.t1 | AT5G08080 | 46.735 | 291 | 139 | 4 | 1 | 277 | 1 | 289 | 1.75e-77 | 239 |
| MS.gene002374.t1 | AT2G18260 | 36.656 | 311 | 176 | 5 | 1 | 297 | 1 | 304 | 2.31e-64 | 205 |
| MS.gene002374.t1 | AT2G18260 | 36.656 | 311 | 176 | 5 | 1 | 297 | 1 | 304 | 2.31e-64 | 205 |
| MS.gene002374.t1 | AT5G08080 | 47.030 | 202 | 102 | 2 | 1 | 199 | 1 | 200 | 7.09e-45 | 152 |
| MS.gene002374.t1 | AT5G08080 | 47.030 | 202 | 102 | 2 | 1 | 199 | 1 | 200 | 7.09e-45 | 152 |
Find 39 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCTTCTACATCATCAATT+TGG | 0.171423 | 2.4:+71926141 | None:intergenic |
| CAAAAGAGTCAAAATCATTA+AGG | 0.241294 | 2.4:-71925934 | MS.gene002374:CDS |
| ACAAGGTAGGGGTCAGATTA+TGG | 0.287097 | 2.4:-71925619 | MS.gene002374:CDS |
| TGCTTCTACATCATCAATTT+GGG | 0.291794 | 2.4:+71926142 | None:intergenic |
| CAACCTTGATAAATTCTTTG+AGG | 0.373925 | 2.4:-71926105 | MS.gene002374:CDS |
| ACTTCAGTGGTTAGTGGATT+GGG | 0.377345 | 2.4:-71925825 | MS.gene002374:CDS |
| AACTTCAGTGGTTAGTGGAT+TGG | 0.416019 | 2.4:-71925826 | MS.gene002374:CDS |
| ATCAAAAGGATTCAAGGAAA+TGG | 0.420191 | 2.4:-71925395 | MS.gene002374:CDS |
| CCCTGGAATGTTTCTATTCG+CGG | 0.422099 | 2.4:+71925881 | None:intergenic |
| GATGGATAAAGATGTTGAAC+AGG | 0.436094 | 2.4:-71925961 | MS.gene002374:CDS |
| AAAAGAGTCAAAATCATTAA+GGG | 0.451614 | 2.4:-71925933 | MS.gene002374:CDS |
| GCCGCGAATAGAAACATTCC+AGG | 0.456499 | 2.4:-71925882 | MS.gene002374:CDS |
| AACATTCCAGGGTGTGGTCC+AGG | 0.464073 | 2.4:-71925870 | MS.gene002374:CDS |
| GTTCTATCTGCTGATGAACC+TGG | 0.467339 | 2.4:+71925852 | None:intergenic |
| GAGAGCATCAAAAGGATTCA+AGG | 0.474516 | 2.4:-71925401 | MS.gene002374:CDS |
| ACAAGAACTTCAGTGGTTAG+TGG | 0.481738 | 2.4:-71925831 | MS.gene002374:CDS |
| ATTGAGAATTTGATATCAAG+TGG | 0.510431 | 2.4:-71925678 | MS.gene002374:CDS |
| CATCCTCAAAGAATTTATCA+AGG | 0.518396 | 2.4:+71926102 | None:intergenic |
| CAAATTGATGATGTAGAAGC+AGG | 0.522039 | 2.4:-71926140 | MS.gene002374:CDS |
| AGCAATTCAAGAACAAGGTA+GGG | 0.529179 | 2.4:-71925631 | MS.gene002374:CDS |
| GAGCAATTCAAGAACAAGGT+AGG | 0.536020 | 2.4:-71925632 | MS.gene002374:CDS |
| CAATGAAAGATCTTAGATCA+AGG | 0.542118 | 2.4:-71925983 | MS.gene002374:CDS |
| GAAAGATCTTAGATCAAGGA+TGG | 0.545537 | 2.4:-71925979 | MS.gene002374:CDS |
| ACAAAGAAACAATTGAAAGA+AGG | 0.546398 | 2.4:-71925740 | MS.gene002374:CDS |
| GATGAACCTGGACCACACCC+TGG | 0.554068 | 2.4:+71925864 | None:intergenic |
| TGAGGCAAGAGAGCATCAAA+AGG | 0.557741 | 2.4:-71925409 | MS.gene002374:CDS |
| TTGACATCAACAATGGAAAG+AGG | 0.573552 | 2.4:+71925328 | None:intergenic |
| AATAGAAACATTCCAGGGTG+TGG | 0.577755 | 2.4:-71925876 | MS.gene002374:CDS |
| CAAAGAGCAATTCAAGAACA+AGG | 0.592503 | 2.4:-71925636 | MS.gene002374:CDS |
| GAAAGAGGAGCACGATGACG+AGG | 0.604139 | 2.4:+71925343 | None:intergenic |
| GCTCTATAGAAAATTACAAG+AGG | 0.608759 | 2.4:-71926042 | MS.gene002374:CDS |
| GGTAGAATTGACATCAACAA+TGG | 0.610196 | 2.4:+71925321 | None:intergenic |
| GCAATTCAAGAACAAGGTAG+GGG | 0.620511 | 2.4:-71925630 | MS.gene002374:CDS |
| AGAATGTGAAAGAAGACATG+AGG | 0.630310 | 2.4:-71926076 | MS.gene002374:CDS |
| AGATAGAACAAGAACTTCAG+TGG | 0.645922 | 2.4:-71925838 | MS.gene002374:CDS |
| AGGTACTGAACAACTTCATG+AGG | 0.661031 | 2.4:-71925427 | MS.gene002374:CDS |
| GCAGCACTTGTTGAGTCACA+AGG | 0.663060 | 2.4:-71925507 | MS.gene002374:CDS |
| CCGCGAATAGAAACATTCCA+GGG | 0.695706 | 2.4:-71925881 | MS.gene002374:CDS |
| TAATTCTACAGAAACAAGTG+AGG | 0.702509 | 2.4:+71925300 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAACTCAAAGATATGA+TGG | - | chr2.4:71925700-71925719 | MS.gene002374:CDS | 20.0% |
| !! | AAAAGAGTCAAAATCATTAA+GGG | - | chr2.4:71925569-71925588 | MS.gene002374:CDS | 20.0% |
| ! | ACAAAGAAACAATTGAAAGA+AGG | - | chr2.4:71925762-71925781 | MS.gene002374:CDS | 25.0% |
| ! | ATTGAGAATTTGATATCAAG+TGG | - | chr2.4:71925824-71925843 | MS.gene002374:CDS | 25.0% |
| ! | CAAAAGAGTCAAAATCATTA+AGG | - | chr2.4:71925568-71925587 | MS.gene002374:CDS | 25.0% |
| !!! | AGAAGGTATTTTACAATAAC+TGG | - | chr2.4:71925779-71925798 | MS.gene002374:CDS | 25.0% |
| !!! | ATATGATGGATGATTTTCAA+GGG | - | chr2.4:71925714-71925733 | MS.gene002374:CDS | 25.0% |
| !!! | TAATGATTTTGACTCTTTTG+AGG | + | chr2.4:71925568-71925587 | None:intergenic | 25.0% |
| ATCAAAAGGATTCAAGGAAA+TGG | - | chr2.4:71926107-71926126 | MS.gene002374:CDS | 30.0% | |
| CAAAAAATACAGTGACTTGA+AGG | - | chr2.4:71925331-71925350 | MS.gene002374:CDS | 30.0% | |
| CAACCTTGATAAATTCTTTG+AGG | - | chr2.4:71925397-71925416 | MS.gene002374:CDS | 30.0% | |
| CAATGAAAGATCTTAGATCA+AGG | - | chr2.4:71925519-71925538 | MS.gene002374:CDS | 30.0% | |
| CATCCTCAAAGAATTTATCA+AGG | + | chr2.4:71925403-71925422 | None:intergenic | 30.0% | |
| GCTCTATAGAAAATTACAAG+AGG | - | chr2.4:71925460-71925479 | MS.gene002374:CDS | 30.0% | |
| TGCTTCTACATCATCAATTT+GGG | + | chr2.4:71925363-71925382 | None:intergenic | 30.0% | |
| ! | TGAGTTGCATCAAGTATTTT+TGG | - | chr2.4:71925967-71925986 | MS.gene002374:CDS | 30.0% |
| !!! | CAAGTCACTGTATTTTTTGA+AGG | + | chr2.4:71925330-71925349 | None:intergenic | 30.0% |
| !!! | CTTTCATTGTTTTAGCATTG+TGG | + | chr2.4:71925508-71925527 | None:intergenic | 30.0% |
| !!! | GATATGATGGATGATTTTCA+AGG | - | chr2.4:71925713-71925732 | MS.gene002374:CDS | 30.0% |
| AGAATGTGAAAGAAGACATG+AGG | - | chr2.4:71925426-71925445 | MS.gene002374:CDS | 35.0% | |
| AGATAGAACAAGAACTTCAG+TGG | - | chr2.4:71925664-71925683 | MS.gene002374:CDS | 35.0% | |
| AGCAATTCAAGAACAAGGTA+GGG | - | chr2.4:71925871-71925890 | MS.gene002374:CDS | 35.0% | |
| CAAAGAGCAATTCAAGAACA+AGG | - | chr2.4:71925866-71925885 | MS.gene002374:CDS | 35.0% | |
| CTGCTTCTACATCATCAATT+TGG | + | chr2.4:71925364-71925383 | None:intergenic | 35.0% | |
| GAAAGATCTTAGATCAAGGA+TGG | - | chr2.4:71925523-71925542 | MS.gene002374:CDS | 35.0% | |
| GATGGATAAAGATGTTGAAC+AGG | - | chr2.4:71925541-71925560 | MS.gene002374:CDS | 35.0% | |
| GGTAGAATTGACATCAACAA+TGG | + | chr2.4:71926184-71926203 | None:intergenic | 35.0% | |
| TTGACATCAACAATGGAAAG+AGG | + | chr2.4:71926177-71926196 | None:intergenic | 35.0% | |
| !! | CAAATTGATGATGTAGAAGC+AGG | - | chr2.4:71925362-71925381 | MS.gene002374:CDS | 35.0% |
| !! | GCTAGTTCTTTTGTTAGAAG+AGG | - | chr2.4:71926055-71926074 | MS.gene002374:CDS | 35.0% |
| !!! | GCATCAAGTATTTTTGGACA+TGG | - | chr2.4:71925973-71925992 | MS.gene002374:CDS | 35.0% |
| AATAGAAACATTCCAGGGTG+TGG | - | chr2.4:71925626-71925645 | MS.gene002374:CDS | 40.0% | |
| ACAAGAACTTCAGTGGTTAG+TGG | - | chr2.4:71925671-71925690 | MS.gene002374:CDS | 40.0% | |
| GAGCAATTCAAGAACAAGGT+AGG | - | chr2.4:71925870-71925889 | MS.gene002374:CDS | 40.0% | |
| GCAATTCAAGAACAAGGTAG+GGG | - | chr2.4:71925872-71925891 | MS.gene002374:CDS | 40.0% | |
| ! | AACTTCAGTGGTTAGTGGAT+TGG | - | chr2.4:71925676-71925695 | MS.gene002374:CDS | 40.0% |
| !! | ACTTCAGTGGTTAGTGGATT+GGG | - | chr2.4:71925677-71925696 | MS.gene002374:CDS | 40.0% |
| !! | AGGTACTGAACAACTTCATG+AGG | - | chr2.4:71926075-71926094 | MS.gene002374:CDS | 40.0% |
| !! | GAGAGCATCAAAAGGATTCA+AGG | - | chr2.4:71926101-71926120 | MS.gene002374:CDS | 40.0% |
| ACAAGGTAGGGGTCAGATTA+TGG | - | chr2.4:71925883-71925902 | MS.gene002374:CDS | 45.0% | |
| CCGCGAATAGAAACATTCCA+GGG | - | chr2.4:71925621-71925640 | MS.gene002374:CDS | 45.0% | |
| GTTCTATCTGCTGATGAACC+TGG | + | chr2.4:71925653-71925672 | None:intergenic | 45.0% | |
| ! | CCCTGGAATGTTTCTATTCG+CGG | + | chr2.4:71925624-71925643 | None:intergenic | 45.0% |
| !! | TGAGGCAAGAGAGCATCAAA+AGG | - | chr2.4:71926093-71926112 | MS.gene002374:CDS | 45.0% |
| GCCGCGAATAGAAACATTCC+AGG | - | chr2.4:71925620-71925639 | MS.gene002374:CDS | 50.0% | |
| ! | GCAGCACTTGTTGAGTCACA+AGG | - | chr2.4:71925995-71926014 | MS.gene002374:CDS | 50.0% |
| AACATTCCAGGGTGTGGTCC+AGG | - | chr2.4:71925632-71925651 | MS.gene002374:CDS | 55.0% | |
| ! | GAAAGAGGAGCACGATGACG+AGG | + | chr2.4:71926162-71926181 | None:intergenic | 55.0% |
| GATGAACCTGGACCACACCC+TGG | + | chr2.4:71925641-71925660 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 71925305 | 71926219 | 71925305 | ID=MS.gene002374 |
| chr2.4 | mRNA | 71925305 | 71926219 | 71925305 | ID=MS.gene002374.t1;Parent=MS.gene002374 |
| chr2.4 | exon | 71925305 | 71926219 | 71925305 | ID=MS.gene002374.t1.exon1;Parent=MS.gene002374.t1 |
| chr2.4 | CDS | 71925305 | 71926219 | 71925305 | ID=cds.MS.gene002374.t1;Parent=MS.gene002374.t1 |
| Gene Sequence |
| Protein sequence |
