Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32192.t1 | GAU45093.1 | 81.6 | 305 | 56 | 0 | 1 | 305 | 1 | 305 | 5.20E-129 | 470.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32192.t1 | O64791 | 71.4 | 304 | 82 | 2 | 1 | 304 | 1 | 299 | 8.4e-112 | 404.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32192.t1 | A0A2Z6NSJ6 | 81.6 | 305 | 56 | 0 | 1 | 305 | 1 | 305 | 3.7e-129 | 470.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene32192 | MS.gene033077 | PPI |
| MS.gene32192 | MS.gene70948 | PPI |
| MS.gene32192 | MS.gene063476 | PPI |
| MS.gene32192 | MS.gene035781 | PPI |
| MS.gene32192 | MS.gene55383 | PPI |
| MS.gene32192 | MS.gene21144 | PPI |
| MS.gene32192 | MS.gene99692 | PPI |
| MS.gene32192 | MS.gene044431 | PPI |
| MS.gene32192 | MS.gene015460 | PPI |
| MS.gene32192 | MS.gene73141 | PPI |
| MS.gene32192 | MS.gene09947 | PPI |
| MS.gene32192 | MS.gene025786 | PPI |
| MS.gene32192 | MS.gene53170 | PPI |
| MS.gene32192 | MS.gene064565 | PPI |
| MS.gene32192 | MS.gene69311 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32192.t1 | MTR_4g090900 | 98.361 | 305 | 5 | 0 | 1 | 305 | 1 | 305 | 0.0 | 619 |
| MS.gene32192.t1 | MTR_2g012270 | 88.487 | 304 | 34 | 1 | 1 | 304 | 1 | 303 | 0.0 | 561 |
| MS.gene32192.t1 | MTR_1g056550 | 58.741 | 286 | 109 | 3 | 1 | 278 | 1 | 285 | 1.30e-120 | 348 |
| MS.gene32192.t1 | MTR_1g056560 | 57.045 | 291 | 114 | 2 | 1 | 280 | 1 | 291 | 1.37e-118 | 344 |
| MS.gene32192.t1 | MTR_7g106420 | 55.000 | 280 | 120 | 1 | 1 | 280 | 1 | 274 | 8.50e-108 | 315 |
| MS.gene32192.t1 | MTR_5g012010 | 50.338 | 296 | 129 | 4 | 1 | 278 | 1 | 296 | 2.25e-99 | 295 |
| MS.gene32192.t1 | MTR_6g028140 | 48.239 | 284 | 139 | 2 | 1 | 280 | 1 | 280 | 7.90e-91 | 273 |
| MS.gene32192.t1 | MTR_2g088700 | 46.643 | 283 | 146 | 1 | 1 | 278 | 1 | 283 | 4.25e-87 | 263 |
| MS.gene32192.t1 | MTR_2g088700 | 42.763 | 304 | 169 | 1 | 1 | 299 | 1 | 304 | 8.45e-86 | 260 |
| MS.gene32192.t1 | MTR_5g020670 | 44.599 | 287 | 142 | 4 | 1 | 279 | 1 | 278 | 5.38e-72 | 224 |
| MS.gene32192.t1 | MTR_8g073900 | 40.476 | 168 | 71 | 3 | 1 | 161 | 1 | 146 | 1.53e-25 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32192.t1 | AT1G61290 | 77.303 | 304 | 64 | 2 | 1 | 304 | 1 | 299 | 5.89e-178 | 493 |
| MS.gene32192.t1 | AT1G61290 | 77.303 | 304 | 64 | 2 | 1 | 304 | 1 | 299 | 5.89e-178 | 493 |
| MS.gene32192.t1 | AT1G11250 | 80.357 | 280 | 49 | 2 | 1 | 280 | 1 | 274 | 7.63e-166 | 462 |
| MS.gene32192.t1 | AT4G03330 | 69.205 | 302 | 91 | 1 | 1 | 300 | 1 | 302 | 7.16e-149 | 420 |
| MS.gene32192.t1 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 5 | 257 | 4.39e-124 | 357 |
| MS.gene32192.t1 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 36 | 288 | 1.17e-123 | 358 |
| MS.gene32192.t1 | AT3G52400 | 55.973 | 293 | 112 | 3 | 1 | 280 | 1 | 289 | 5.38e-107 | 315 |
| MS.gene32192.t1 | AT3G03800 | 47.872 | 282 | 140 | 2 | 1 | 278 | 1 | 279 | 3.86e-88 | 266 |
| MS.gene32192.t1 | AT3G03800 | 47.872 | 282 | 140 | 2 | 1 | 278 | 1 | 279 | 3.86e-88 | 266 |
| MS.gene32192.t1 | AT1G08560 | 46.735 | 291 | 137 | 4 | 1 | 278 | 1 | 286 | 4.21e-86 | 261 |
| MS.gene32192.t1 | AT5G08080 | 49.643 | 280 | 137 | 2 | 1 | 278 | 1 | 278 | 1.86e-81 | 249 |
| MS.gene32192.t1 | AT5G08080 | 49.643 | 280 | 137 | 2 | 1 | 278 | 1 | 278 | 1.86e-81 | 249 |
| MS.gene32192.t1 | AT5G08080 | 47.423 | 291 | 138 | 4 | 1 | 278 | 1 | 289 | 2.84e-79 | 243 |
| MS.gene32192.t1 | AT2G18260 | 36.656 | 311 | 177 | 5 | 1 | 298 | 1 | 304 | 2.70e-63 | 202 |
| MS.gene32192.t1 | AT2G18260 | 36.656 | 311 | 177 | 5 | 1 | 298 | 1 | 304 | 2.70e-63 | 202 |
| MS.gene32192.t1 | AT5G08080 | 46.040 | 202 | 105 | 2 | 1 | 200 | 1 | 200 | 2.91e-44 | 150 |
| MS.gene32192.t1 | AT5G08080 | 46.040 | 202 | 105 | 2 | 1 | 200 | 1 | 200 | 2.91e-44 | 150 |
Find 39 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCAGTTGTTAGTGGTTT+AGG | 0.190941 | 4.4:+22881942 | MS.gene32192:CDS |
| AAACGTGTCAAAATTATTAA+AGG | 0.208067 | 4.4:+22881834 | MS.gene32192:CDS |
| CTTCAGTTGTTAGTGGTTTA+GGG | 0.301650 | 4.4:+22881943 | MS.gene32192:CDS |
| TCAAATAATGGACACTATTT+CGG | 0.305761 | 4.4:+22882160 | MS.gene32192:CDS |
| GAGTGCAACAAAAGAATTCT+AGG | 0.314563 | 4.4:+22882366 | MS.gene32192:CDS |
| TCGTGCTCGAAGTTCTTTCA+TGG | 0.315609 | 4.4:-22881784 | None:intergenic |
| ACAAGGTAGGGGTCAAATAA+TGG | 0.355079 | 4.4:+22882148 | MS.gene32192:CDS |
| AAGAGGGACGGAACAACTTC+AGG | 0.385821 | 4.4:+22882337 | MS.gene32192:CDS |
| AGAAAGACATGATGCTGTTA+AGG | 0.392149 | 4.4:+22882190 | MS.gene32192:CDS |
| ACTAGAACTTCAGTTGTTAG+TGG | 0.396328 | 4.4:+22881936 | MS.gene32192:CDS |
| AAACAATTGAACAATTGTAT+AGG | 0.433871 | 4.4:+22881712 | MS.gene32192:CDS |
| TACCTTGATGATGTCGAGGC+AGG | 0.443621 | 4.4:+22881624 | MS.gene32192:CDS |
| GCAGCTAATAGAAACATTCC+AGG | 0.458362 | 4.4:+22881885 | MS.gene32192:CDS |
| TAAAGGAAAACTCGAAGCAT+TGG | 0.462694 | 4.4:+22881851 | MS.gene32192:CDS |
| AACATTCCAGGGTGTGGTCC+AGG | 0.464073 | 4.4:+22881897 | MS.gene32192:CDS |
| GAAGAAACTCAAAGATATGA+TGG | 0.479536 | 4.4:+22881965 | MS.gene32192:CDS |
| GTTCTATCTGCTGATGAACC+TGG | 0.498169 | 4.4:-22881915 | None:intergenic |
| AAGTGAAAATTTCATGCAAA+GGG | 0.501636 | 4.4:+22882115 | MS.gene32192:CDS |
| GGCAATTCAAGAACAAGGTA+GGG | 0.504105 | 4.4:+22882136 | MS.gene32192:CDS |
| AACAAAAGAATTCTAGGAAG+TGG | 0.524800 | 4.4:+22882372 | MS.gene32192:CDS |
| GGGCAATTCAAGAACAAGGT+AGG | 0.529449 | 4.4:+22882135 | MS.gene32192:CDS |
| ATAGAAAATTTGATATCAAG+TGG | 0.532572 | 4.4:+22882089 | MS.gene32192:CDS |
| ACCTTGATGATGTCGAGGCA+GGG | 0.538277 | 4.4:+22881625 | MS.gene32192:CDS |
| TACAATAACTGGAGAGAAAG+CGG | 0.540895 | 4.4:+22882055 | MS.gene32192:CDS |
| GAAAGAACTTCGAGCACGAA+TGG | 0.541312 | 4.4:+22881788 | MS.gene32192:CDS |
| GCATCTTGAGTATAAGGAAA+CGG | 0.542829 | 4.4:+22882016 | MS.gene32192:CDS |
| CGATAAATTCTTCGAAGATG+TGG | 0.548904 | 4.4:+22881668 | MS.gene32192:CDS |
| ATTGTATAGGAAATTGAAAG+AGG | 0.552639 | 4.4:+22881725 | MS.gene32192:CDS |
| GATGAACCTGGACCACACCC+TGG | 0.554068 | 4.4:-22881903 | None:intergenic |
| AAAGTGAAAATTTCATGCAA+AGG | 0.560085 | 4.4:+22882114 | MS.gene32192:CDS |
| AATAGAAACATTCCAGGGTG+TGG | 0.577755 | 4.4:+22881891 | MS.gene32192:CDS |
| TCCCTGCCTCGACATCATCA+AGG | 0.592333 | 4.4:-22881626 | None:intergenic |
| CAAAGGGCAATTCAAGAACA+AGG | 0.597990 | 4.4:+22882131 | MS.gene32192:CDS |
| AGCTTACCTTGATGATGTCG+AGG | 0.625446 | 4.4:+22881620 | MS.gene32192:CDS |
| GCAATTCAAGAACAAGGTAG+GGG | 0.626558 | 4.4:+22882137 | MS.gene32192:CDS |
| GCAGCTTTGGTAGAGTCTCA+AGG | 0.630011 | 4.4:+22882260 | MS.gene32192:CDS |
| AAGAACTAGCATGTGCAACA+TGG | 0.643271 | 4.4:-22882307 | None:intergenic |
| CAGCTAATAGAAACATTCCA+GGG | 0.687559 | 4.4:+22881886 | MS.gene32192:CDS |
| TCTTGAGTATAAGGAAACGG+TGG | 0.715657 | 4.4:+22882019 | MS.gene32192:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAATTGAACAATTGTAT+AGG | + | chr4.4:22881712-22881731 | MS.gene32192:CDS | 20.0% |
| !! | AAACGTGTCAAAATTATTAA+AGG | + | chr4.4:22881834-22881853 | MS.gene32192:CDS | 20.0% |
| !! | ATAGAAAATTTGATATCAAG+TGG | + | chr4.4:22882089-22882108 | MS.gene32192:CDS | 20.0% |
| !! | TAGAACAATACCAAAAATTA+TGG | - | chr4.4:22882408-22882427 | None:intergenic | 20.0% |
| !!! | TAATAATTTTGACACGTTTA+AGG | - | chr4.4:22881833-22881852 | None:intergenic | 20.0% |
| ! | AAAAATGCATCTTGAGTATA+AGG | + | chr4.4:22882010-22882029 | MS.gene32192:CDS | 25.0% |
| ! | AAAGTGAAAATTTCATGCAA+AGG | + | chr4.4:22882114-22882133 | MS.gene32192:CDS | 25.0% |
| ! | AAGTGAAAATTTCATGCAAA+GGG | + | chr4.4:22882115-22882134 | MS.gene32192:CDS | 25.0% |
| ! | ATTGTATAGGAAATTGAAAG+AGG | + | chr4.4:22881725-22881744 | MS.gene32192:CDS | 25.0% |
| !! | ACATGTTATGCCATAATTTT+TGG | + | chr4.4:22882395-22882414 | MS.gene32192:CDS | 25.0% |
| !! | CGAAGATATTTTACAATAAC+TGG | + | chr4.4:22882044-22882063 | MS.gene32192:CDS | 25.0% |
| !!! | TCAAATAATGGACACTATTT+CGG | + | chr4.4:22882160-22882179 | MS.gene32192:CDS | 25.0% |
| GAAGAAACTCAAAGATATGA+TGG | + | chr4.4:22881965-22881984 | MS.gene32192:CDS | 30.0% | |
| ! | AACAAAAGAATTCTAGGAAG+TGG | + | chr4.4:22882372-22882391 | MS.gene32192:CDS | 30.0% |
| !! | CAAAAAGTACACTGATTTGA+AGG | + | chr4.4:22881593-22881612 | MS.gene32192:CDS | 30.0% |
| !! | CAAATCAGTGTACTTTTTGA+AGG | - | chr4.4:22881592-22881611 | None:intergenic | 30.0% |
| !! | CTAGTTCTTTTGTAAGAAGA+GGG | + | chr4.4:22882321-22882340 | MS.gene32192:CDS | 30.0% |
| !!! | GATATGATGGATGATTTTCA+AGG | + | chr4.4:22881978-22881997 | MS.gene32192:CDS | 30.0% |
| ACTAGAACTTCAGTTGTTAG+TGG | + | chr4.4:22881936-22881955 | MS.gene32192:CDS | 35.0% | |
| ACTTCAGTTGTTAGTGGTTT+AGG | + | chr4.4:22881942-22881961 | MS.gene32192:CDS | 35.0% | |
| AGAAAGACATGATGCTGTTA+AGG | + | chr4.4:22882190-22882209 | MS.gene32192:CDS | 35.0% | |
| CAGCTAATAGAAACATTCCA+GGG | + | chr4.4:22881886-22881905 | MS.gene32192:CDS | 35.0% | |
| CGATAAATTCTTCGAAGATG+TGG | + | chr4.4:22881668-22881687 | MS.gene32192:CDS | 35.0% | |
| CTTCAGTTGTTAGTGGTTTA+GGG | + | chr4.4:22881943-22881962 | MS.gene32192:CDS | 35.0% | |
| GAGTGCAACAAAAGAATTCT+AGG | + | chr4.4:22882366-22882385 | MS.gene32192:CDS | 35.0% | |
| GCATCTTGAGTATAAGGAAA+CGG | + | chr4.4:22882016-22882035 | MS.gene32192:CDS | 35.0% | |
| GGTAAAAATGATGTCAAGAG+AGG | - | chr4.4:22882449-22882468 | None:intergenic | 35.0% | |
| TAAAGGAAAACTCGAAGCAT+TGG | + | chr4.4:22881851-22881870 | MS.gene32192:CDS | 35.0% | |
| TACAATAACTGGAGAGAAAG+CGG | + | chr4.4:22882055-22882074 | MS.gene32192:CDS | 35.0% | |
| ! | TGAGTTGCATCAAGTGTTTT+TGG | + | chr4.4:22882232-22882251 | MS.gene32192:CDS | 35.0% |
| ! | TTCTTTTGTAAGAAGAGGGA+CGG | + | chr4.4:22882325-22882344 | MS.gene32192:CDS | 35.0% |
| !! | GCTAGTTCTTTTGTAAGAAG+AGG | + | chr4.4:22882320-22882339 | MS.gene32192:CDS | 35.0% |
| !!! | GCATCAAGTGTTTTTGGATA+TGG | + | chr4.4:22882238-22882257 | MS.gene32192:CDS | 35.0% |
| AAGAACTAGCATGTGCAACA+TGG | - | chr4.4:22882310-22882329 | None:intergenic | 40.0% | |
| AATAGAAACATTCCAGGGTG+TGG | + | chr4.4:22881891-22881910 | MS.gene32192:CDS | 40.0% | |
| ACAAGGTAGGGGTCAAATAA+TGG | + | chr4.4:22882148-22882167 | MS.gene32192:CDS | 40.0% | |
| CAAAGGGCAATTCAAGAACA+AGG | + | chr4.4:22882131-22882150 | MS.gene32192:CDS | 40.0% | |
| GCAATTCAAGAACAAGGTAG+GGG | + | chr4.4:22882137-22882156 | MS.gene32192:CDS | 40.0% | |
| GCAGCTAATAGAAACATTCC+AGG | + | chr4.4:22881885-22881904 | MS.gene32192:CDS | 40.0% | |
| GGCAATTCAAGAACAAGGTA+GGG | + | chr4.4:22882136-22882155 | MS.gene32192:CDS | 40.0% | |
| TCTTGAGTATAAGGAAACGG+TGG | + | chr4.4:22882019-22882038 | MS.gene32192:CDS | 40.0% | |
| !! | GTTTTTGGATATGGCAGCTT+TGG | + | chr4.4:22882247-22882266 | MS.gene32192:CDS | 40.0% |
| !!! | CATGGTTTTAGCATTGTGCA+CGG | - | chr4.4:22881769-22881788 | None:intergenic | 40.0% |
| GAAAGAACTTCGAGCACGAA+TGG | + | chr4.4:22881788-22881807 | MS.gene32192:CDS | 45.0% | |
| GGGCAATTCAAGAACAAGGT+AGG | + | chr4.4:22882135-22882154 | MS.gene32192:CDS | 45.0% | |
| GTTCTATCTGCTGATGAACC+TGG | - | chr4.4:22881918-22881937 | None:intergenic | 45.0% | |
| TCGTGCTCGAAGTTCTTTCA+TGG | - | chr4.4:22881787-22881806 | None:intergenic | 45.0% | |
| !! | AGCTTACCTTGATGATGTCG+AGG | + | chr4.4:22881620-22881639 | MS.gene32192:CDS | 45.0% |
| AAGAGGGACGGAACAACTTC+AGG | + | chr4.4:22882337-22882356 | MS.gene32192:CDS | 50.0% | |
| ! | GCAGCTTTGGTAGAGTCTCA+AGG | + | chr4.4:22882260-22882279 | MS.gene32192:CDS | 50.0% |
| !! | ACCTTGATGATGTCGAGGCA+GGG | + | chr4.4:22881625-22881644 | MS.gene32192:CDS | 50.0% |
| !! | TACCTTGATGATGTCGAGGC+AGG | + | chr4.4:22881624-22881643 | MS.gene32192:CDS | 50.0% |
| AACATTCCAGGGTGTGGTCC+AGG | + | chr4.4:22881897-22881916 | MS.gene32192:CDS | 55.0% | |
| TCCCTGCCTCGACATCATCA+AGG | - | chr4.4:22881629-22881648 | None:intergenic | 55.0% | |
| GATGAACCTGGACCACACCC+TGG | - | chr4.4:22881906-22881925 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 22881567 | 22882484 | 22881567 | ID=MS.gene32192 |
| chr4.4 | mRNA | 22881567 | 22882484 | 22881567 | ID=MS.gene32192.t1;Parent=MS.gene32192 |
| chr4.4 | exon | 22881567 | 22882484 | 22881567 | ID=MS.gene32192.t1.exon1;Parent=MS.gene32192.t1 |
| chr4.4 | CDS | 22881567 | 22882484 | 22881567 | ID=cds.MS.gene32192.t1;Parent=MS.gene32192.t1 |
| Gene Sequence |
| Protein sequence |
