Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052542.t1 | XP_003608218.1 | 99 | 305 | 3 | 0 | 1 | 305 | 1 | 305 | 2.70E-130 | 474.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052542.t1 | O64791 | 74.2 | 283 | 72 | 1 | 1 | 283 | 1 | 282 | 2.1e-110 | 400.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052542.t1 | G7JRS4 | 99.0 | 305 | 3 | 0 | 1 | 305 | 1 | 305 | 2.0e-130 | 474.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035781 | MS.gene052542 | PPI |
| MS.gene052542 | MS.gene015460 | PPI |
| MS.gene73141 | MS.gene052542 | PPI |
| MS.gene052542 | MS.gene53170 | PPI |
| MS.gene052542 | MS.gene69311 | PPI |
| MS.gene064565 | MS.gene052542 | PPI |
| MS.gene025786 | MS.gene052542 | PPI |
| MS.gene052542 | MS.gene09947 | PPI |
| MS.gene052542 | MS.gene063476 | PPI |
| MS.gene033077 | MS.gene052542 | PPI |
| MS.gene70948 | MS.gene052542 | PPI |
| MS.gene21144 | MS.gene052542 | PPI |
| MS.gene052542 | MS.gene55383 | PPI |
| MS.gene052542 | MS.gene99692 | PPI |
| MS.gene052542 | MS.gene044431 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052542.t1 | MTR_4g090900 | 99.016 | 305 | 3 | 0 | 1 | 305 | 1 | 305 | 0.0 | 622 |
| MS.gene052542.t1 | MTR_2g012270 | 88.816 | 304 | 33 | 1 | 1 | 304 | 1 | 303 | 0.0 | 563 |
| MS.gene052542.t1 | MTR_1g056550 | 58.741 | 286 | 109 | 3 | 1 | 278 | 1 | 285 | 1.06e-120 | 348 |
| MS.gene052542.t1 | MTR_1g056560 | 57.045 | 291 | 114 | 2 | 1 | 280 | 1 | 291 | 1.15e-118 | 345 |
| MS.gene052542.t1 | MTR_7g106420 | 55.000 | 280 | 120 | 1 | 1 | 280 | 1 | 274 | 9.79e-108 | 315 |
| MS.gene052542.t1 | MTR_5g012010 | 50.338 | 296 | 129 | 4 | 1 | 278 | 1 | 296 | 2.51e-99 | 295 |
| MS.gene052542.t1 | MTR_6g028140 | 48.239 | 284 | 139 | 2 | 1 | 280 | 1 | 280 | 9.00e-91 | 272 |
| MS.gene052542.t1 | MTR_2g088700 | 46.643 | 283 | 146 | 1 | 1 | 278 | 1 | 283 | 4.64e-87 | 263 |
| MS.gene052542.t1 | MTR_2g088700 | 43.522 | 301 | 165 | 1 | 1 | 296 | 1 | 301 | 5.18e-86 | 260 |
| MS.gene052542.t1 | MTR_5g020670 | 44.599 | 287 | 142 | 4 | 1 | 279 | 1 | 278 | 4.29e-72 | 224 |
| MS.gene052542.t1 | MTR_8g073900 | 40.476 | 168 | 71 | 3 | 1 | 161 | 1 | 146 | 1.39e-25 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052542.t1 | AT1G61290 | 77.632 | 304 | 63 | 2 | 1 | 304 | 1 | 299 | 1.62e-178 | 495 |
| MS.gene052542.t1 | AT1G61290 | 77.632 | 304 | 63 | 2 | 1 | 304 | 1 | 299 | 1.62e-178 | 495 |
| MS.gene052542.t1 | AT1G11250 | 80.357 | 280 | 49 | 2 | 1 | 280 | 1 | 274 | 3.87e-166 | 463 |
| MS.gene052542.t1 | AT4G03330 | 69.536 | 302 | 90 | 1 | 1 | 300 | 1 | 302 | 4.42e-149 | 421 |
| MS.gene052542.t1 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 5 | 257 | 3.34e-124 | 358 |
| MS.gene052542.t1 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 36 | 288 | 1.37e-123 | 357 |
| MS.gene052542.t1 | AT3G52400 | 55.973 | 293 | 112 | 3 | 1 | 280 | 1 | 289 | 6.99e-107 | 315 |
| MS.gene052542.t1 | AT3G03800 | 47.872 | 282 | 140 | 2 | 1 | 278 | 1 | 279 | 3.14e-88 | 266 |
| MS.gene052542.t1 | AT3G03800 | 47.872 | 282 | 140 | 2 | 1 | 278 | 1 | 279 | 3.14e-88 | 266 |
| MS.gene052542.t1 | AT1G08560 | 46.735 | 291 | 137 | 4 | 1 | 278 | 1 | 286 | 4.59e-86 | 261 |
| MS.gene052542.t1 | AT5G08080 | 49.643 | 280 | 137 | 2 | 1 | 278 | 1 | 278 | 1.92e-81 | 249 |
| MS.gene052542.t1 | AT5G08080 | 49.643 | 280 | 137 | 2 | 1 | 278 | 1 | 278 | 1.92e-81 | 249 |
| MS.gene052542.t1 | AT5G08080 | 47.423 | 291 | 138 | 4 | 1 | 278 | 1 | 289 | 2.58e-79 | 244 |
| MS.gene052542.t1 | AT2G18260 | 36.977 | 311 | 176 | 5 | 1 | 298 | 1 | 304 | 1.26e-63 | 203 |
| MS.gene052542.t1 | AT2G18260 | 36.977 | 311 | 176 | 5 | 1 | 298 | 1 | 304 | 1.26e-63 | 203 |
| MS.gene052542.t1 | AT5G08080 | 46.040 | 202 | 105 | 2 | 1 | 200 | 1 | 200 | 3.10e-44 | 150 |
| MS.gene052542.t1 | AT5G08080 | 46.040 | 202 | 105 | 2 | 1 | 200 | 1 | 200 | 3.10e-44 | 150 |
Find 43 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCAGTTGTTAGTGGTTT+AGG | 0.190941 | 4.3:+23162600 | MS.gene052542:CDS |
| AAACGTGTCAAAATTATTAA+AGG | 0.208067 | 4.3:+23162492 | MS.gene052542:CDS |
| TTTCATGCAAAGGGCAATTC+AGG | 0.249484 | 4.3:+23162782 | MS.gene052542:CDS |
| CTTCAGTTGTTAGTGGTTTA+GGG | 0.301650 | 4.3:+23162601 | MS.gene052542:CDS |
| TCAAATAATGGACACTATTT+CGG | 0.305761 | 4.3:+23162818 | MS.gene052542:CDS |
| GAGTGCAACAAAAGAATTCT+AGG | 0.314563 | 4.3:+23163024 | MS.gene052542:CDS |
| TCGTGCTCGAAGTTCTTTCA+TGG | 0.315609 | 4.3:-23162442 | None:intergenic |
| ACATGTTATGCCATAATTCT+TGG | 0.328356 | 4.3:+23163053 | MS.gene052542:CDS |
| TAGAACAATACCAAGAATTA+TGG | 0.331526 | 4.3:-23163063 | None:intergenic |
| ACAAGGTAGGGGTCAAATAA+TGG | 0.363432 | 4.3:+23162806 | MS.gene052542:CDS |
| AAGAGGGACGGAACAACTTC+AGG | 0.385821 | 4.3:+23162995 | MS.gene052542:CDS |
| AGAAAGACATGATGCTGTTA+AGG | 0.392149 | 4.3:+23162848 | MS.gene052542:CDS |
| ACTAGAACTTCAGTTGTTAG+TGG | 0.396328 | 4.3:+23162594 | MS.gene052542:CDS |
| AAACAATTGAACAATTGTAT+AGG | 0.433871 | 4.3:+23162370 | MS.gene052542:CDS |
| TACCTTGATGATGTCGAGGC+AGG | 0.443621 | 4.3:+23162282 | MS.gene052542:CDS |
| GCAGCTAATAGAAACATTCC+AGG | 0.458362 | 4.3:+23162543 | MS.gene052542:CDS |
| TAAAGGAAAACTCGAAGCAT+TGG | 0.462694 | 4.3:+23162509 | MS.gene052542:CDS |
| AACATTCCAGGGTGTGGTCC+AGG | 0.464073 | 4.3:+23162555 | MS.gene052542:CDS |
| GAAGAAACTCAAAGATATGA+TGG | 0.479536 | 4.3:+23162623 | MS.gene052542:CDS |
| GGCAATTCAGGAACAAGGTA+GGG | 0.479587 | 4.3:+23162794 | MS.gene052542:CDS |
| GTTCTATCTGCTGATGAACC+TGG | 0.498169 | 4.3:-23162573 | None:intergenic |
| AAGTGAAAATTTCATGCAAA+GGG | 0.501636 | 4.3:+23162773 | MS.gene052542:CDS |
| AACAAAAGAATTCTAGGAAG+TGG | 0.524800 | 4.3:+23163030 | MS.gene052542:CDS |
| ATAGAAAATTTGATATCAAG+TGG | 0.532572 | 4.3:+23162747 | MS.gene052542:CDS |
| ACCTTGATGATGTCGAGGCA+GGG | 0.538277 | 4.3:+23162283 | MS.gene052542:CDS |
| TACAATAACTGGAGAGAAAG+CGG | 0.540895 | 4.3:+23162713 | MS.gene052542:CDS |
| GAAAGAACTTCGAGCACGAA+TGG | 0.541312 | 4.3:+23162446 | MS.gene052542:CDS |
| GCATCTTGAGTATAAGGAAA+CGG | 0.542829 | 4.3:+23162674 | MS.gene052542:CDS |
| CGATAAATTCTTCGAAGATG+TGG | 0.548904 | 4.3:+23162326 | MS.gene052542:CDS |
| ATTGTATAGGAAATTGAAAG+AGG | 0.552639 | 4.3:+23162383 | MS.gene052542:CDS |
| GATGAACCTGGACCACACCC+TGG | 0.554068 | 4.3:-23162561 | None:intergenic |
| GGGCAATTCAGGAACAAGGT+AGG | 0.558247 | 4.3:+23162793 | MS.gene052542:CDS |
| AAAGTGAAAATTTCATGCAA+AGG | 0.560085 | 4.3:+23162772 | MS.gene052542:CDS |
| AATAGAAACATTCCAGGGTG+TGG | 0.577755 | 4.3:+23162549 | MS.gene052542:CDS |
| GCAATTCAGGAACAAGGTAG+GGG | 0.584430 | 4.3:+23162795 | MS.gene052542:CDS |
| GGTAAAAGTGATGTCAAGAG+AGG | 0.586377 | 4.3:-23163104 | None:intergenic |
| TCCCTGCCTCGACATCATCA+AGG | 0.592333 | 4.3:-23162284 | None:intergenic |
| CAAAGGGCAATTCAGGAACA+AGG | 0.596826 | 4.3:+23162789 | MS.gene052542:CDS |
| AGCTTACCTTGATGATGTCG+AGG | 0.625446 | 4.3:+23162278 | MS.gene052542:CDS |
| GCAGCTTTGGTAGAGTCTCA+AGG | 0.630011 | 4.3:+23162918 | MS.gene052542:CDS |
| AAGAACTAGCATGTGCAACA+TGG | 0.643271 | 4.3:-23162965 | None:intergenic |
| CAGCTAATAGAAACATTCCA+GGG | 0.687559 | 4.3:+23162544 | MS.gene052542:CDS |
| TCTTGAGTATAAGGAAACGG+TGG | 0.715657 | 4.3:+23162677 | MS.gene052542:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAATTGAACAATTGTAT+AGG | + | chr4.3:23162370-23162389 | MS.gene052542:CDS | 20.0% |
| !! | AAACGTGTCAAAATTATTAA+AGG | + | chr4.3:23162492-23162511 | MS.gene052542:CDS | 20.0% |
| !! | ATAGAAAATTTGATATCAAG+TGG | + | chr4.3:23162747-23162766 | MS.gene052542:CDS | 20.0% |
| !!! | TAATAATTTTGACACGTTTA+AGG | - | chr4.3:23162491-23162510 | None:intergenic | 20.0% |
| ! | AAAAATGCATCTTGAGTATA+AGG | + | chr4.3:23162668-23162687 | MS.gene052542:CDS | 25.0% |
| ! | AAAGTGAAAATTTCATGCAA+AGG | + | chr4.3:23162772-23162791 | MS.gene052542:CDS | 25.0% |
| ! | AAGTGAAAATTTCATGCAAA+GGG | + | chr4.3:23162773-23162792 | MS.gene052542:CDS | 25.0% |
| ! | ATTGTATAGGAAATTGAAAG+AGG | + | chr4.3:23162383-23162402 | MS.gene052542:CDS | 25.0% |
| ! | TAGAACAATACCAAGAATTA+TGG | - | chr4.3:23163066-23163085 | None:intergenic | 25.0% |
| !! | CGAAGATATTTTACAATAAC+TGG | + | chr4.3:23162702-23162721 | MS.gene052542:CDS | 25.0% |
| !!! | TCAAATAATGGACACTATTT+CGG | + | chr4.3:23162818-23162837 | MS.gene052542:CDS | 25.0% |
| ACATGTTATGCCATAATTCT+TGG | + | chr4.3:23163053-23163072 | MS.gene052542:CDS | 30.0% | |
| GAAGAAACTCAAAGATATGA+TGG | + | chr4.3:23162623-23162642 | MS.gene052542:CDS | 30.0% | |
| ! | AACAAAAGAATTCTAGGAAG+TGG | + | chr4.3:23163030-23163049 | MS.gene052542:CDS | 30.0% |
| !! | CAAAAAGTACACTGATTTGA+AGG | + | chr4.3:23162251-23162270 | MS.gene052542:CDS | 30.0% |
| !! | CAAATCAGTGTACTTTTTGA+AGG | - | chr4.3:23162250-23162269 | None:intergenic | 30.0% |
| !! | CTAGTTCTTTTGTAAGAAGA+GGG | + | chr4.3:23162979-23162998 | MS.gene052542:CDS | 30.0% |
| !!! | GATATGATGGATGATTTTCA+AGG | + | chr4.3:23162636-23162655 | MS.gene052542:CDS | 30.0% |
| ACTAGAACTTCAGTTGTTAG+TGG | + | chr4.3:23162594-23162613 | MS.gene052542:CDS | 35.0% | |
| ACTTCAGTTGTTAGTGGTTT+AGG | + | chr4.3:23162600-23162619 | MS.gene052542:CDS | 35.0% | |
| AGAAAGACATGATGCTGTTA+AGG | + | chr4.3:23162848-23162867 | MS.gene052542:CDS | 35.0% | |
| CAGCTAATAGAAACATTCCA+GGG | + | chr4.3:23162544-23162563 | MS.gene052542:CDS | 35.0% | |
| CGATAAATTCTTCGAAGATG+TGG | + | chr4.3:23162326-23162345 | MS.gene052542:CDS | 35.0% | |
| CTTCAGTTGTTAGTGGTTTA+GGG | + | chr4.3:23162601-23162620 | MS.gene052542:CDS | 35.0% | |
| GAGTGCAACAAAAGAATTCT+AGG | + | chr4.3:23163024-23163043 | MS.gene052542:CDS | 35.0% | |
| GCATCTTGAGTATAAGGAAA+CGG | + | chr4.3:23162674-23162693 | MS.gene052542:CDS | 35.0% | |
| TAAAGGAAAACTCGAAGCAT+TGG | + | chr4.3:23162509-23162528 | MS.gene052542:CDS | 35.0% | |
| TACAATAACTGGAGAGAAAG+CGG | + | chr4.3:23162713-23162732 | MS.gene052542:CDS | 35.0% | |
| ! | TGAGTTGCATCAAGTGTTTT+TGG | + | chr4.3:23162890-23162909 | MS.gene052542:CDS | 35.0% |
| ! | TTCTTTTGTAAGAAGAGGGA+CGG | + | chr4.3:23162983-23163002 | MS.gene052542:CDS | 35.0% |
| !! | GCTAGTTCTTTTGTAAGAAG+AGG | + | chr4.3:23162978-23162997 | MS.gene052542:CDS | 35.0% |
| !!! | GCATCAAGTGTTTTTGGATA+TGG | + | chr4.3:23162896-23162915 | MS.gene052542:CDS | 35.0% |
| AAGAACTAGCATGTGCAACA+TGG | - | chr4.3:23162968-23162987 | None:intergenic | 40.0% | |
| AATAGAAACATTCCAGGGTG+TGG | + | chr4.3:23162549-23162568 | MS.gene052542:CDS | 40.0% | |
| ACAAGGTAGGGGTCAAATAA+TGG | + | chr4.3:23162806-23162825 | MS.gene052542:CDS | 40.0% | |
| GCAGCTAATAGAAACATTCC+AGG | + | chr4.3:23162543-23162562 | MS.gene052542:CDS | 40.0% | |
| GGTAAAAGTGATGTCAAGAG+AGG | - | chr4.3:23163107-23163126 | None:intergenic | 40.0% | |
| TCTTGAGTATAAGGAAACGG+TGG | + | chr4.3:23162677-23162696 | MS.gene052542:CDS | 40.0% | |
| TTTCATGCAAAGGGCAATTC+AGG | + | chr4.3:23162782-23162801 | MS.gene052542:CDS | 40.0% | |
| !! | GTTTTTGGATATGGCAGCTT+TGG | + | chr4.3:23162905-23162924 | MS.gene052542:CDS | 40.0% |
| !!! | CATGGTTTTAGCATTGTGCA+CGG | - | chr4.3:23162427-23162446 | None:intergenic | 40.0% |
| CAAAGGGCAATTCAGGAACA+AGG | + | chr4.3:23162789-23162808 | MS.gene052542:CDS | 45.0% | |
| GAAAGAACTTCGAGCACGAA+TGG | + | chr4.3:23162446-23162465 | MS.gene052542:CDS | 45.0% | |
| GCAATTCAGGAACAAGGTAG+GGG | + | chr4.3:23162795-23162814 | MS.gene052542:CDS | 45.0% | |
| GGCAATTCAGGAACAAGGTA+GGG | + | chr4.3:23162794-23162813 | MS.gene052542:CDS | 45.0% | |
| GTTCTATCTGCTGATGAACC+TGG | - | chr4.3:23162576-23162595 | None:intergenic | 45.0% | |
| TCGTGCTCGAAGTTCTTTCA+TGG | - | chr4.3:23162445-23162464 | None:intergenic | 45.0% | |
| !! | AGCTTACCTTGATGATGTCG+AGG | + | chr4.3:23162278-23162297 | MS.gene052542:CDS | 45.0% |
| AAGAGGGACGGAACAACTTC+AGG | + | chr4.3:23162995-23163014 | MS.gene052542:CDS | 50.0% | |
| GGGCAATTCAGGAACAAGGT+AGG | + | chr4.3:23162793-23162812 | MS.gene052542:CDS | 50.0% | |
| ! | GCAGCTTTGGTAGAGTCTCA+AGG | + | chr4.3:23162918-23162937 | MS.gene052542:CDS | 50.0% |
| !! | ACCTTGATGATGTCGAGGCA+GGG | + | chr4.3:23162283-23162302 | MS.gene052542:CDS | 50.0% |
| !! | TACCTTGATGATGTCGAGGC+AGG | + | chr4.3:23162282-23162301 | MS.gene052542:CDS | 50.0% |
| AACATTCCAGGGTGTGGTCC+AGG | + | chr4.3:23162555-23162574 | MS.gene052542:CDS | 55.0% | |
| TCCCTGCCTCGACATCATCA+AGG | - | chr4.3:23162287-23162306 | None:intergenic | 55.0% | |
| GATGAACCTGGACCACACCC+TGG | - | chr4.3:23162564-23162583 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 23162225 | 23163142 | 23162225 | ID=MS.gene052542 |
| chr4.3 | mRNA | 23162225 | 23163142 | 23162225 | ID=MS.gene052542.t1;Parent=MS.gene052542 |
| chr4.3 | exon | 23162225 | 23163142 | 23162225 | ID=MS.gene052542.t1.exon1;Parent=MS.gene052542.t1 |
| chr4.3 | CDS | 23162225 | 23163142 | 23162225 | ID=cds.MS.gene052542.t1;Parent=MS.gene052542.t1 |
| Gene Sequence |
| Protein sequence |
