Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33736.t1 | XP_003608218.1 | 97 | 305 | 9 | 0 | 1 | 305 | 1 | 305 | 2.10E-130 | 475.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33736.t1 | O64791 | 74.1 | 286 | 73 | 1 | 1 | 286 | 1 | 285 | 1.6e-110 | 400.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33736.t1 | G7JRS4 | 97.0 | 305 | 9 | 0 | 1 | 305 | 1 | 305 | 1.5e-130 | 475.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene33736 | MS.gene035781 | PPI |
| MS.gene33736 | MS.gene55383 | PPI |
| MS.gene33736 | MS.gene21144 | PPI |
| MS.gene33736 | MS.gene015460 | PPI |
| MS.gene33736 | MS.gene99692 | PPI |
| MS.gene33736 | MS.gene044431 | PPI |
| MS.gene33736 | MS.gene69311 | PPI |
| MS.gene33736 | MS.gene70948 | PPI |
| MS.gene33736 | MS.gene73141 | PPI |
| MS.gene33736 | MS.gene09947 | PPI |
| MS.gene33736 | MS.gene025786 | PPI |
| MS.gene33736 | MS.gene53170 | PPI |
| MS.gene33736 | MS.gene064565 | PPI |
| MS.gene33736 | MS.gene033077 | PPI |
| MS.gene33736 | MS.gene063476 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33736.t1 | MTR_4g090900 | 99.344 | 305 | 2 | 0 | 1 | 305 | 1 | 305 | 0.0 | 624 |
| MS.gene33736.t1 | MTR_2g012270 | 89.803 | 304 | 30 | 1 | 1 | 304 | 1 | 303 | 0.0 | 569 |
| MS.gene33736.t1 | MTR_1g056550 | 58.741 | 286 | 109 | 3 | 1 | 278 | 1 | 285 | 8.96e-121 | 348 |
| MS.gene33736.t1 | MTR_1g056560 | 57.045 | 291 | 114 | 2 | 1 | 280 | 1 | 291 | 1.06e-118 | 345 |
| MS.gene33736.t1 | MTR_7g106420 | 54.643 | 280 | 121 | 1 | 1 | 280 | 1 | 274 | 3.05e-107 | 314 |
| MS.gene33736.t1 | MTR_5g012010 | 50.000 | 296 | 130 | 4 | 1 | 278 | 1 | 296 | 1.89e-99 | 295 |
| MS.gene33736.t1 | MTR_6g028140 | 47.872 | 282 | 139 | 2 | 1 | 278 | 1 | 278 | 1.07e-88 | 267 |
| MS.gene33736.t1 | MTR_2g088700 | 47.350 | 283 | 144 | 1 | 1 | 278 | 1 | 283 | 1.83e-88 | 266 |
| MS.gene33736.t1 | MTR_2g088700 | 44.667 | 300 | 159 | 2 | 1 | 295 | 1 | 298 | 8.62e-87 | 262 |
| MS.gene33736.t1 | MTR_5g020670 | 44.251 | 287 | 143 | 4 | 1 | 279 | 1 | 278 | 9.35e-72 | 224 |
| MS.gene33736.t1 | MTR_8g073900 | 39.881 | 168 | 72 | 3 | 1 | 161 | 1 | 146 | 2.58e-25 | 99.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33736.t1 | AT1G61290 | 77.632 | 304 | 63 | 2 | 1 | 304 | 1 | 299 | 2.68e-178 | 494 |
| MS.gene33736.t1 | AT1G61290 | 77.632 | 304 | 63 | 2 | 1 | 304 | 1 | 299 | 2.68e-178 | 494 |
| MS.gene33736.t1 | AT1G11250 | 80.357 | 280 | 49 | 2 | 1 | 280 | 1 | 274 | 6.84e-166 | 463 |
| MS.gene33736.t1 | AT4G03330 | 68.874 | 302 | 92 | 1 | 1 | 300 | 1 | 302 | 3.39e-148 | 418 |
| MS.gene33736.t1 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 5 | 257 | 2.43e-124 | 358 |
| MS.gene33736.t1 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 36 | 288 | 1.33e-123 | 357 |
| MS.gene33736.t1 | AT3G52400 | 55.631 | 293 | 113 | 3 | 1 | 280 | 1 | 289 | 2.17e-106 | 313 |
| MS.gene33736.t1 | AT3G03800 | 48.227 | 282 | 139 | 2 | 1 | 278 | 1 | 279 | 8.98e-89 | 267 |
| MS.gene33736.t1 | AT3G03800 | 48.227 | 282 | 139 | 2 | 1 | 278 | 1 | 279 | 8.98e-89 | 267 |
| MS.gene33736.t1 | AT1G08560 | 46.392 | 291 | 138 | 4 | 1 | 278 | 1 | 286 | 5.18e-86 | 261 |
| MS.gene33736.t1 | AT5G08080 | 50.000 | 280 | 136 | 2 | 1 | 278 | 1 | 278 | 6.78e-82 | 250 |
| MS.gene33736.t1 | AT5G08080 | 50.000 | 280 | 136 | 2 | 1 | 278 | 1 | 278 | 6.78e-82 | 250 |
| MS.gene33736.t1 | AT5G08080 | 47.766 | 291 | 137 | 4 | 1 | 278 | 1 | 289 | 7.91e-80 | 245 |
| MS.gene33736.t1 | AT2G18260 | 37.621 | 311 | 174 | 5 | 1 | 298 | 1 | 304 | 1.50e-64 | 206 |
| MS.gene33736.t1 | AT2G18260 | 37.621 | 311 | 174 | 5 | 1 | 298 | 1 | 304 | 1.50e-64 | 206 |
| MS.gene33736.t1 | AT5G08080 | 46.535 | 202 | 104 | 2 | 1 | 200 | 1 | 200 | 8.94e-45 | 152 |
| MS.gene33736.t1 | AT5G08080 | 46.535 | 202 | 104 | 2 | 1 | 200 | 1 | 200 | 8.94e-45 | 152 |
Find 44 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCTGTTGTTAGTGGTTT+AGG | 0.186018 | 4.4:+23263396 | MS.gene33736:CDS |
| AAACGTGTCAAAATTATTAA+AGG | 0.208067 | 4.4:+23263288 | MS.gene33736:CDS |
| CTTCTGTTGTTAGTGGTTTA+GGG | 0.303686 | 4.4:+23263397 | MS.gene33736:CDS |
| TCAAATAATGGATACTATTT+CGG | 0.307200 | 4.4:+23263614 | MS.gene33736:CDS |
| AAGAATGCAACTTGAGTATA+AGG | 0.308882 | 4.4:+23263464 | MS.gene33736:CDS |
| GAGTGCAACAAAAGAATTCT+AGG | 0.314563 | 4.4:+23263820 | MS.gene33736:CDS |
| TCGTGCTCGAAGTTCTTTCA+TGG | 0.315609 | 4.4:-23263238 | None:intergenic |
| ACATGTTATGTCATAATTCT+TGG | 0.328356 | 4.4:+23263849 | MS.gene33736:CDS |
| ACAAGGTAGGGGTCAAATAA+TGG | 0.353617 | 4.4:+23263602 | MS.gene33736:CDS |
| ACTAGAACTTCTGTTGTTAG+TGG | 0.379314 | 4.4:+23263390 | MS.gene33736:CDS |
| AAGAGGGACGGAACAACTTC+AGG | 0.385821 | 4.4:+23263791 | MS.gene33736:CDS |
| AGAAAGACATGATGCTGTTA+AGG | 0.392149 | 4.4:+23263644 | MS.gene33736:CDS |
| TACCTCATTTGTTAAAATGA+AGG | 0.394311 | 4.4:+23263919 | MS.gene33736:CDS |
| AAACAATTGAACAATTGTAT+AGG | 0.433871 | 4.4:+23263166 | MS.gene33736:CDS |
| TACCTTGATGATGTCGAGGC+AGG | 0.443621 | 4.4:+23263078 | MS.gene33736:CDS |
| GCAGCTAATAGAAACATTCC+AGG | 0.458362 | 4.4:+23263339 | MS.gene33736:CDS |
| TAAAGGAAAACTCGAAGCAT+TGG | 0.462694 | 4.4:+23263305 | MS.gene33736:CDS |
| AACATTCCAGGGTGTGGTCC+AGG | 0.464073 | 4.4:+23263351 | MS.gene33736:CDS |
| GAAGAAACTCAAAGATATGA+TGG | 0.479536 | 4.4:+23263419 | MS.gene33736:CDS |
| GTTCTATCTGCTGATGAACC+TGG | 0.498169 | 4.4:-23263369 | None:intergenic |
| GGCAATTCAAGAACAAGGTA+GGG | 0.517496 | 4.4:+23263590 | MS.gene33736:CDS |
| AACAAAAGAATTCTAGGAAG+TGG | 0.524800 | 4.4:+23263826 | MS.gene33736:CDS |
| ACCTTGATGATGTCGAGGCA+GGG | 0.538277 | 4.4:+23263079 | MS.gene33736:CDS |
| TACAATAACGGGAGAGAAAG+CGG | 0.538348 | 4.4:+23263509 | MS.gene33736:CDS |
| GAAAGAACTTCGAGCACGAA+TGG | 0.541312 | 4.4:+23263242 | MS.gene33736:CDS |
| GGGCAATTCAAGAACAAGGT+AGG | 0.542840 | 4.4:+23263589 | MS.gene33736:CDS |
| ATAGAGAATTTGATATCAAG+TGG | 0.548451 | 4.4:+23263543 | MS.gene33736:CDS |
| CGATAAATTCTTCGAAGATG+TGG | 0.548904 | 4.4:+23263122 | MS.gene33736:CDS |
| ATTGTATAGGAAATTGAAAG+AGG | 0.552639 | 4.4:+23263179 | MS.gene33736:CDS |
| GATGAACCTGGACCACACCC+TGG | 0.554068 | 4.4:-23263357 | None:intergenic |
| GCAACTTGAGTATAAGGAAA+CGG | 0.555427 | 4.4:+23263470 | MS.gene33736:CDS |
| AATAGAAACATTCCAGGGTG+TGG | 0.577755 | 4.4:+23263345 | MS.gene33736:CDS |
| GGTAAAAGTGATGTCAAGAG+AGG | 0.586377 | 4.4:-23263900 | None:intergenic |
| AAGTGAAAATTTCATGCAGA+GGG | 0.592035 | 4.4:+23263569 | MS.gene33736:CDS |
| TCCCTGCCTCGACATCATCA+AGG | 0.592333 | 4.4:-23263080 | None:intergenic |
| CAGAGGGCAATTCAAGAACA+AGG | 0.604810 | 4.4:+23263585 | MS.gene33736:CDS |
| AAAGTGAAAATTTCATGCAG+AGG | 0.618688 | 4.4:+23263568 | MS.gene33736:CDS |
| AGCTTACCTTGATGATGTCG+AGG | 0.625446 | 4.4:+23263074 | MS.gene33736:CDS |
| GCAATTCAAGAACAAGGTAG+GGG | 0.629512 | 4.4:+23263591 | MS.gene33736:CDS |
| GCAGCTTTGGTAGAGTCTCA+AGG | 0.630011 | 4.4:+23263714 | MS.gene33736:CDS |
| AAGAACTAGCATGTGCAACA+TGG | 0.643271 | 4.4:-23263761 | None:intergenic |
| ATAAGGAAACGGTGGAACGA+AGG | 0.658457 | 4.4:+23263481 | MS.gene33736:CDS |
| CAGCTAATAGAAACATTCCA+GGG | 0.687559 | 4.4:+23263340 | MS.gene33736:CDS |
| ACTTGAGTATAAGGAAACGG+TGG | 0.721256 | 4.4:+23263473 | MS.gene33736:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAATTGAACAATTGTAT+AGG | + | chr4.4:23263166-23263185 | MS.gene33736:CDS | 20.0% |
| !! | AAACGTGTCAAAATTATTAA+AGG | + | chr4.4:23263288-23263307 | MS.gene33736:CDS | 20.0% |
| !!! | TAATAATTTTGACACGTTTA+AGG | - | chr4.4:23263287-23263306 | None:intergenic | 20.0% |
| !!! | TCAAATAATGGATACTATTT+CGG | + | chr4.4:23263614-23263633 | MS.gene33736:CDS | 20.0% |
| ! | ACATGTTATGTCATAATTCT+TGG | + | chr4.4:23263849-23263868 | MS.gene33736:CDS | 25.0% |
| ! | ATAGAGAATTTGATATCAAG+TGG | + | chr4.4:23263543-23263562 | MS.gene33736:CDS | 25.0% |
| ! | ATTGTATAGGAAATTGAAAG+AGG | + | chr4.4:23263179-23263198 | MS.gene33736:CDS | 25.0% |
| !!! | ACGAAGGTATTTTACAATAA+CGG | + | chr4.4:23263497-23263516 | MS.gene33736:CDS | 25.0% |
| AAAGTGAAAATTTCATGCAG+AGG | + | chr4.4:23263568-23263587 | MS.gene33736:CDS | 30.0% | |
| AAGAATGCAACTTGAGTATA+AGG | + | chr4.4:23263464-23263483 | MS.gene33736:CDS | 30.0% | |
| AAGTGAAAATTTCATGCAGA+GGG | + | chr4.4:23263569-23263588 | MS.gene33736:CDS | 30.0% | |
| GAAGAAACTCAAAGATATGA+TGG | + | chr4.4:23263419-23263438 | MS.gene33736:CDS | 30.0% | |
| ! | AACAAAAGAATTCTAGGAAG+TGG | + | chr4.4:23263826-23263845 | MS.gene33736:CDS | 30.0% |
| !! | CAAAAAGTACACTGATTTGA+AGG | + | chr4.4:23263047-23263066 | MS.gene33736:CDS | 30.0% |
| !! | CAAATCAGTGTACTTTTTGA+AGG | - | chr4.4:23263046-23263065 | None:intergenic | 30.0% |
| !! | CTAGTTCTTTTGTAAGAAGA+GGG | + | chr4.4:23263775-23263794 | MS.gene33736:CDS | 30.0% |
| !!! | CGAAGGTATTTTACAATAAC+GGG | + | chr4.4:23263498-23263517 | MS.gene33736:CDS | 30.0% |
| !!! | GATATGATGGATGATTTTCA+AGG | + | chr4.4:23263432-23263451 | MS.gene33736:CDS | 30.0% |
| ACTAGAACTTCTGTTGTTAG+TGG | + | chr4.4:23263390-23263409 | MS.gene33736:CDS | 35.0% | |
| ACTTCTGTTGTTAGTGGTTT+AGG | + | chr4.4:23263396-23263415 | MS.gene33736:CDS | 35.0% | |
| AGAAAGACATGATGCTGTTA+AGG | + | chr4.4:23263644-23263663 | MS.gene33736:CDS | 35.0% | |
| CAGCTAATAGAAACATTCCA+GGG | + | chr4.4:23263340-23263359 | MS.gene33736:CDS | 35.0% | |
| CGATAAATTCTTCGAAGATG+TGG | + | chr4.4:23263122-23263141 | MS.gene33736:CDS | 35.0% | |
| CTTCTGTTGTTAGTGGTTTA+GGG | + | chr4.4:23263397-23263416 | MS.gene33736:CDS | 35.0% | |
| GAGTGCAACAAAAGAATTCT+AGG | + | chr4.4:23263820-23263839 | MS.gene33736:CDS | 35.0% | |
| GCAACTTGAGTATAAGGAAA+CGG | + | chr4.4:23263470-23263489 | MS.gene33736:CDS | 35.0% | |
| TAAAGGAAAACTCGAAGCAT+TGG | + | chr4.4:23263305-23263324 | MS.gene33736:CDS | 35.0% | |
| ! | TGAGTTGCATCAAGTGTTTT+TGG | + | chr4.4:23263686-23263705 | MS.gene33736:CDS | 35.0% |
| ! | TTCTTTTGTAAGAAGAGGGA+CGG | + | chr4.4:23263779-23263798 | MS.gene33736:CDS | 35.0% |
| !! | GCTAGTTCTTTTGTAAGAAG+AGG | + | chr4.4:23263774-23263793 | MS.gene33736:CDS | 35.0% |
| !!! | GCATCAAGTGTTTTTGGATA+TGG | + | chr4.4:23263692-23263711 | MS.gene33736:CDS | 35.0% |
| AAGAACTAGCATGTGCAACA+TGG | - | chr4.4:23263764-23263783 | None:intergenic | 40.0% | |
| AATAGAAACATTCCAGGGTG+TGG | + | chr4.4:23263345-23263364 | MS.gene33736:CDS | 40.0% | |
| ACAAGGTAGGGGTCAAATAA+TGG | + | chr4.4:23263602-23263621 | MS.gene33736:CDS | 40.0% | |
| ACTTGAGTATAAGGAAACGG+TGG | + | chr4.4:23263473-23263492 | MS.gene33736:CDS | 40.0% | |
| GCAATTCAAGAACAAGGTAG+GGG | + | chr4.4:23263591-23263610 | MS.gene33736:CDS | 40.0% | |
| GCAGCTAATAGAAACATTCC+AGG | + | chr4.4:23263339-23263358 | MS.gene33736:CDS | 40.0% | |
| GGCAATTCAAGAACAAGGTA+GGG | + | chr4.4:23263590-23263609 | MS.gene33736:CDS | 40.0% | |
| GGTAAAAGTGATGTCAAGAG+AGG | - | chr4.4:23263903-23263922 | None:intergenic | 40.0% | |
| ! | TACAATAACGGGAGAGAAAG+CGG | + | chr4.4:23263509-23263528 | MS.gene33736:CDS | 40.0% |
| !! | GTTTTTGGATATGGCAGCTT+TGG | + | chr4.4:23263701-23263720 | MS.gene33736:CDS | 40.0% |
| !!! | CATGGTTTTAGCATTGTGCA+CGG | - | chr4.4:23263223-23263242 | None:intergenic | 40.0% |
| ATAAGGAAACGGTGGAACGA+AGG | + | chr4.4:23263481-23263500 | MS.gene33736:CDS | 45.0% | |
| CAGAGGGCAATTCAAGAACA+AGG | + | chr4.4:23263585-23263604 | MS.gene33736:CDS | 45.0% | |
| GAAAGAACTTCGAGCACGAA+TGG | + | chr4.4:23263242-23263261 | MS.gene33736:CDS | 45.0% | |
| GGGCAATTCAAGAACAAGGT+AGG | + | chr4.4:23263589-23263608 | MS.gene33736:CDS | 45.0% | |
| GTTCTATCTGCTGATGAACC+TGG | - | chr4.4:23263372-23263391 | None:intergenic | 45.0% | |
| TCGTGCTCGAAGTTCTTTCA+TGG | - | chr4.4:23263241-23263260 | None:intergenic | 45.0% | |
| !! | AGCTTACCTTGATGATGTCG+AGG | + | chr4.4:23263074-23263093 | MS.gene33736:CDS | 45.0% |
| AAGAGGGACGGAACAACTTC+AGG | + | chr4.4:23263791-23263810 | MS.gene33736:CDS | 50.0% | |
| ! | GCAGCTTTGGTAGAGTCTCA+AGG | + | chr4.4:23263714-23263733 | MS.gene33736:CDS | 50.0% |
| !! | ACCTTGATGATGTCGAGGCA+GGG | + | chr4.4:23263079-23263098 | MS.gene33736:CDS | 50.0% |
| !! | TACCTTGATGATGTCGAGGC+AGG | + | chr4.4:23263078-23263097 | MS.gene33736:CDS | 50.0% |
| AACATTCCAGGGTGTGGTCC+AGG | + | chr4.4:23263351-23263370 | MS.gene33736:CDS | 55.0% | |
| TCCCTGCCTCGACATCATCA+AGG | - | chr4.4:23263083-23263102 | None:intergenic | 55.0% | |
| GATGAACCTGGACCACACCC+TGG | - | chr4.4:23263360-23263379 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 23263021 | 23263938 | 23263021 | ID=MS.gene33736 |
| chr4.4 | mRNA | 23263021 | 23263938 | 23263021 | ID=MS.gene33736.t1;Parent=MS.gene33736 |
| chr4.4 | exon | 23263021 | 23263938 | 23263021 | ID=MS.gene33736.t1.exon1;Parent=MS.gene33736.t1 |
| chr4.4 | CDS | 23263021 | 23263938 | 23263021 | ID=cds.MS.gene33736.t1;Parent=MS.gene33736.t1 |
| Gene Sequence |
| Protein sequence |
