Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01756.t1 | XP_024632050.1 | 99.7 | 304 | 1 | 0 | 1 | 304 | 1 | 304 | 2.10E-151 | 545 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01756.t1 | O64791 | 80.9 | 282 | 54 | 0 | 1 | 282 | 1 | 282 | 1.2e-123 | 444.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01756.t1 | G7ILD5 | 99.7 | 304 | 1 | 0 | 1 | 304 | 1 | 304 | 1.5e-151 | 545.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene01756 | MS.gene69311 | PPI |
| MS.gene70948 | MS.gene01756 | PPI |
| MS.gene033077 | MS.gene01756 | PPI |
| MS.gene21144 | MS.gene01756 | PPI |
| MS.gene01756 | MS.gene09947 | PPI |
| MS.gene01756 | MS.gene025786 | PPI |
| MS.gene01756 | MS.gene53170 | PPI |
| MS.gene01756 | MS.gene063476 | PPI |
| MS.gene01756 | MS.gene55383 | PPI |
| MS.gene035781 | MS.gene01756 | PPI |
| MS.gene01756 | MS.gene015460 | PPI |
| MS.gene73141 | MS.gene01756 | PPI |
| MS.gene01756 | MS.gene99692 | PPI |
| MS.gene01756 | MS.gene044431 | PPI |
| MS.gene064565 | MS.gene01756 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01756.t1 | MTR_2g012270 | 99.671 | 304 | 1 | 0 | 1 | 304 | 1 | 304 | 0.0 | 622 |
| MS.gene01756.t1 | MTR_4g090900 | 90.000 | 280 | 27 | 1 | 1 | 279 | 1 | 280 | 0.0 | 525 |
| MS.gene01756.t1 | MTR_1g056550 | 58.042 | 286 | 110 | 2 | 1 | 277 | 1 | 285 | 6.13e-122 | 352 |
| MS.gene01756.t1 | MTR_1g056560 | 56.701 | 291 | 114 | 1 | 1 | 279 | 1 | 291 | 4.61e-119 | 345 |
| MS.gene01756.t1 | MTR_7g106420 | 57.490 | 247 | 105 | 0 | 33 | 279 | 28 | 274 | 3.20e-105 | 309 |
| MS.gene01756.t1 | MTR_5g012010 | 49.324 | 296 | 131 | 4 | 1 | 277 | 1 | 296 | 7.76e-99 | 293 |
| MS.gene01756.t1 | MTR_2g088700 | 44.444 | 306 | 161 | 3 | 1 | 300 | 1 | 303 | 2.52e-87 | 264 |
| MS.gene01756.t1 | MTR_6g028140 | 46.953 | 279 | 145 | 2 | 1 | 277 | 1 | 278 | 2.24e-86 | 261 |
| MS.gene01756.t1 | MTR_2g088700 | 45.936 | 283 | 147 | 1 | 1 | 277 | 1 | 283 | 6.28e-86 | 260 |
| MS.gene01756.t1 | MTR_5g020670 | 43.206 | 287 | 145 | 4 | 1 | 278 | 1 | 278 | 3.75e-70 | 219 |
| MS.gene01756.t1 | MTR_8g073900 | 39.286 | 168 | 72 | 3 | 1 | 160 | 1 | 146 | 2.35e-23 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01756.t1 | AT1G61290 | 77.961 | 304 | 63 | 1 | 1 | 304 | 1 | 300 | 6.06e-180 | 498 |
| MS.gene01756.t1 | AT1G61290 | 77.961 | 304 | 63 | 1 | 1 | 304 | 1 | 300 | 6.06e-180 | 498 |
| MS.gene01756.t1 | AT1G11250 | 79.211 | 279 | 53 | 1 | 1 | 279 | 1 | 274 | 4.53e-166 | 463 |
| MS.gene01756.t1 | AT4G03330 | 68.543 | 302 | 92 | 1 | 1 | 299 | 1 | 302 | 1.16e-146 | 414 |
| MS.gene01756.t1 | AT3G11820 | 65.992 | 247 | 84 | 0 | 31 | 277 | 11 | 257 | 9.78e-120 | 347 |
| MS.gene01756.t1 | AT3G11820 | 63.566 | 258 | 94 | 0 | 20 | 277 | 31 | 288 | 2.19e-119 | 347 |
| MS.gene01756.t1 | AT3G52400 | 54.671 | 289 | 121 | 2 | 1 | 279 | 1 | 289 | 3.56e-105 | 310 |
| MS.gene01756.t1 | AT3G03800 | 47.872 | 282 | 139 | 2 | 1 | 277 | 1 | 279 | 2.49e-88 | 266 |
| MS.gene01756.t1 | AT3G03800 | 47.872 | 282 | 139 | 2 | 1 | 277 | 1 | 279 | 2.49e-88 | 266 |
| MS.gene01756.t1 | AT1G08560 | 46.048 | 291 | 138 | 4 | 1 | 277 | 1 | 286 | 3.17e-85 | 258 |
| MS.gene01756.t1 | AT5G08080 | 49.643 | 280 | 136 | 2 | 1 | 277 | 1 | 278 | 1.14e-80 | 246 |
| MS.gene01756.t1 | AT5G08080 | 49.643 | 280 | 136 | 2 | 1 | 277 | 1 | 278 | 1.14e-80 | 246 |
| MS.gene01756.t1 | AT5G08080 | 46.735 | 291 | 139 | 4 | 1 | 277 | 1 | 289 | 1.75e-77 | 239 |
| MS.gene01756.t1 | AT2G18260 | 36.656 | 311 | 176 | 5 | 1 | 297 | 1 | 304 | 2.31e-64 | 205 |
| MS.gene01756.t1 | AT2G18260 | 36.656 | 311 | 176 | 5 | 1 | 297 | 1 | 304 | 2.31e-64 | 205 |
| MS.gene01756.t1 | AT5G08080 | 47.030 | 202 | 102 | 2 | 1 | 199 | 1 | 200 | 7.09e-45 | 152 |
| MS.gene01756.t1 | AT5G08080 | 47.030 | 202 | 102 | 2 | 1 | 199 | 1 | 200 | 7.09e-45 | 152 |
Find 41 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCTTCTACATCATCAATT+TGG | 0.171423 | 2.3:+71831945 | None:intergenic |
| CAAAAGAGTCAAAATCATTA+AGG | 0.241294 | 2.3:-71831738 | MS.gene01756:CDS |
| TGCTTCTACATCATCAATTT+GGG | 0.291794 | 2.3:+71831946 | None:intergenic |
| ACAGGGTAGGGGTCAGATTA+TGG | 0.293358 | 2.3:-71831423 | MS.gene01756:CDS |
| CAACCTTGATAAATTCTTTG+AGG | 0.373925 | 2.3:-71831909 | MS.gene01756:CDS |
| ACTTCAGTGGTTAGTGGATT+GGG | 0.377345 | 2.3:-71831629 | MS.gene01756:CDS |
| GCAAAGAGCAATTCAAGAAC+AGG | 0.380268 | 2.3:-71831441 | MS.gene01756:CDS |
| AGAAAGACATGATGCTGTTA+AGG | 0.392149 | 2.3:-71831381 | MS.gene01756:CDS |
| AACTTCAGTGGTTAGTGGAT+TGG | 0.416019 | 2.3:-71831630 | MS.gene01756:CDS |
| ATCAAAAGGATTCAAGGAAA+TGG | 0.420191 | 2.3:-71831199 | MS.gene01756:CDS |
| CCCTGGAATGTTTCTATTCG+CGG | 0.422099 | 2.3:+71831685 | None:intergenic |
| GATGGATAAAGATGTTGAAC+AGG | 0.436094 | 2.3:-71831765 | MS.gene01756:CDS |
| AAAAGAGTCAAAATCATTAA+GGG | 0.451614 | 2.3:-71831737 | MS.gene01756:CDS |
| GCCGCGAATAGAAACATTCC+AGG | 0.456499 | 2.3:-71831686 | MS.gene01756:CDS |
| AACATTCCAGGGTGTGGTCC+AGG | 0.464073 | 2.3:-71831674 | MS.gene01756:CDS |
| GTTCTATCTGCTGATGAACC+TGG | 0.467339 | 2.3:+71831656 | None:intergenic |
| GAGAGCATCAAAAGGATTCA+AGG | 0.474516 | 2.3:-71831205 | MS.gene01756:CDS |
| ACAAGAACTTCAGTGGTTAG+TGG | 0.481738 | 2.3:-71831635 | MS.gene01756:CDS |
| ATTGAGAATTTGATATCAAG+TGG | 0.510431 | 2.3:-71831482 | MS.gene01756:CDS |
| AGCAATTCAAGAACAGGGTA+GGG | 0.517828 | 2.3:-71831435 | MS.gene01756:CDS |
| CATCCTCAAAGAATTTATCA+AGG | 0.518396 | 2.3:+71831906 | None:intergenic |
| CAAATTGATGATGTAGAAGC+AGG | 0.522039 | 2.3:-71831944 | MS.gene01756:CDS |
| CAATGAAAGATCTTAGATCA+AGG | 0.542118 | 2.3:-71831787 | MS.gene01756:CDS |
| GAAAGATCTTAGATCAAGGA+TGG | 0.545537 | 2.3:-71831783 | MS.gene01756:CDS |
| ACAAAGAAACAATTGAAAGA+AGG | 0.546398 | 2.3:-71831544 | MS.gene01756:CDS |
| GAGCAATTCAAGAACAGGGT+AGG | 0.552739 | 2.3:-71831436 | MS.gene01756:CDS |
| GATGAACCTGGACCACACCC+TGG | 0.554068 | 2.3:+71831668 | None:intergenic |
| TGAGGCAAGAGAGCATCAAA+AGG | 0.557741 | 2.3:-71831213 | MS.gene01756:CDS |
| CAAAGAGCAATTCAAGAACA+GGG | 0.572498 | 2.3:-71831440 | MS.gene01756:CDS |
| TTGACATCAACAATGGAAAG+AGG | 0.573552 | 2.3:+71831132 | None:intergenic |
| AATAGAAACATTCCAGGGTG+TGG | 0.577755 | 2.3:-71831680 | MS.gene01756:CDS |
| GAAAGAGGAGCACGATGACG+AGG | 0.604139 | 2.3:+71831147 | None:intergenic |
| GGTAGAATTGACATCAACAA+TGG | 0.610196 | 2.3:+71831125 | None:intergenic |
| GCAATTCAAGAACAGGGTAG+GGG | 0.610861 | 2.3:-71831434 | MS.gene01756:CDS |
| GCTCTACAGAAAATTACAAG+AGG | 0.619853 | 2.3:-71831846 | MS.gene01756:CDS |
| AGAATGTGAAAGAAGACATG+AGG | 0.630310 | 2.3:-71831880 | MS.gene01756:CDS |
| AGATAGAACAAGAACTTCAG+TGG | 0.645922 | 2.3:-71831642 | MS.gene01756:CDS |
| AGGTACTGAACAACTTCATG+AGG | 0.661031 | 2.3:-71831231 | MS.gene01756:CDS |
| GCAGCACTTGTTGAGTCACA+AGG | 0.663060 | 2.3:-71831311 | MS.gene01756:CDS |
| CCGCGAATAGAAACATTCCA+GGG | 0.695706 | 2.3:-71831685 | MS.gene01756:CDS |
| TAATTCTACAGAAACAAGTG+AGG | 0.702509 | 2.3:+71831104 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAACTCAAAGATATGA+TGG | - | chr2.3:71831504-71831523 | MS.gene01756:CDS | 20.0% |
| !! | AAAAGAGTCAAAATCATTAA+GGG | - | chr2.3:71831373-71831392 | MS.gene01756:CDS | 20.0% |
| ! | ACAAAGAAACAATTGAAAGA+AGG | - | chr2.3:71831566-71831585 | MS.gene01756:CDS | 25.0% |
| ! | ATTGAGAATTTGATATCAAG+TGG | - | chr2.3:71831628-71831647 | MS.gene01756:CDS | 25.0% |
| ! | CAAAAGAGTCAAAATCATTA+AGG | - | chr2.3:71831372-71831391 | MS.gene01756:CDS | 25.0% |
| !! | TGAATTGCATCAAGTATTTT+TGG | - | chr2.3:71831771-71831790 | MS.gene01756:CDS | 25.0% |
| !!! | AGAAGGTATTTTACAATAAC+TGG | - | chr2.3:71831583-71831602 | MS.gene01756:CDS | 25.0% |
| !!! | ATATGATGGATGATTTTCAA+GGG | - | chr2.3:71831518-71831537 | MS.gene01756:CDS | 25.0% |
| !!! | TAATGATTTTGACTCTTTTG+AGG | + | chr2.3:71831372-71831391 | None:intergenic | 25.0% |
| ATCAAAAGGATTCAAGGAAA+TGG | - | chr2.3:71831911-71831930 | MS.gene01756:CDS | 30.0% | |
| CAAAAAATACAGTGACTTGA+AGG | - | chr2.3:71831135-71831154 | MS.gene01756:CDS | 30.0% | |
| CAACCTTGATAAATTCTTTG+AGG | - | chr2.3:71831201-71831220 | MS.gene01756:CDS | 30.0% | |
| CAATGAAAGATCTTAGATCA+AGG | - | chr2.3:71831323-71831342 | MS.gene01756:CDS | 30.0% | |
| CATCCTCAAAGAATTTATCA+AGG | + | chr2.3:71831207-71831226 | None:intergenic | 30.0% | |
| TGCTTCTACATCATCAATTT+GGG | + | chr2.3:71831167-71831186 | None:intergenic | 30.0% | |
| !!! | CAAGTCACTGTATTTTTTGA+AGG | + | chr2.3:71831134-71831153 | None:intergenic | 30.0% |
| !!! | CTTTCATTGTTTTAGCATTG+TGG | + | chr2.3:71831312-71831331 | None:intergenic | 30.0% |
| !!! | GATATGATGGATGATTTTCA+AGG | - | chr2.3:71831517-71831536 | MS.gene01756:CDS | 30.0% |
| AGAAAGACATGATGCTGTTA+AGG | - | chr2.3:71831729-71831748 | MS.gene01756:CDS | 35.0% | |
| AGAATGTGAAAGAAGACATG+AGG | - | chr2.3:71831230-71831249 | MS.gene01756:CDS | 35.0% | |
| AGATAGAACAAGAACTTCAG+TGG | - | chr2.3:71831468-71831487 | MS.gene01756:CDS | 35.0% | |
| CAAAGAGCAATTCAAGAACA+GGG | - | chr2.3:71831670-71831689 | MS.gene01756:CDS | 35.0% | |
| CTGCTTCTACATCATCAATT+TGG | + | chr2.3:71831168-71831187 | None:intergenic | 35.0% | |
| GAAAGATCTTAGATCAAGGA+TGG | - | chr2.3:71831327-71831346 | MS.gene01756:CDS | 35.0% | |
| GATGGATAAAGATGTTGAAC+AGG | - | chr2.3:71831345-71831364 | MS.gene01756:CDS | 35.0% | |
| GCTCTACAGAAAATTACAAG+AGG | - | chr2.3:71831264-71831283 | MS.gene01756:CDS | 35.0% | |
| GGTAGAATTGACATCAACAA+TGG | + | chr2.3:71831988-71832007 | None:intergenic | 35.0% | |
| TTGACATCAACAATGGAAAG+AGG | + | chr2.3:71831981-71832000 | None:intergenic | 35.0% | |
| !! | CAAATTGATGATGTAGAAGC+AGG | - | chr2.3:71831166-71831185 | MS.gene01756:CDS | 35.0% |
| !! | GCTAGTTCTTTTGTTAGAAG+AGG | - | chr2.3:71831859-71831878 | MS.gene01756:CDS | 35.0% |
| !!! | GCATCAAGTATTTTTGGACA+TGG | - | chr2.3:71831777-71831796 | MS.gene01756:CDS | 35.0% |
| AATAGAAACATTCCAGGGTG+TGG | - | chr2.3:71831430-71831449 | MS.gene01756:CDS | 40.0% | |
| ACAAGAACTTCAGTGGTTAG+TGG | - | chr2.3:71831475-71831494 | MS.gene01756:CDS | 40.0% | |
| AGCAATTCAAGAACAGGGTA+GGG | - | chr2.3:71831675-71831694 | MS.gene01756:CDS | 40.0% | |
| GCAAAGAGCAATTCAAGAAC+AGG | - | chr2.3:71831669-71831688 | MS.gene01756:CDS | 40.0% | |
| ! | AACTTCAGTGGTTAGTGGAT+TGG | - | chr2.3:71831480-71831499 | MS.gene01756:CDS | 40.0% |
| !! | ACTTCAGTGGTTAGTGGATT+GGG | - | chr2.3:71831481-71831500 | MS.gene01756:CDS | 40.0% |
| !! | AGGTACTGAACAACTTCATG+AGG | - | chr2.3:71831879-71831898 | MS.gene01756:CDS | 40.0% |
| !! | GAGAGCATCAAAAGGATTCA+AGG | - | chr2.3:71831905-71831924 | MS.gene01756:CDS | 40.0% |
| CCGCGAATAGAAACATTCCA+GGG | - | chr2.3:71831425-71831444 | MS.gene01756:CDS | 45.0% | |
| GAGCAATTCAAGAACAGGGT+AGG | - | chr2.3:71831674-71831693 | MS.gene01756:CDS | 45.0% | |
| GCAATTCAAGAACAGGGTAG+GGG | - | chr2.3:71831676-71831695 | MS.gene01756:CDS | 45.0% | |
| GTTCTATCTGCTGATGAACC+TGG | + | chr2.3:71831457-71831476 | None:intergenic | 45.0% | |
| ! | CCCTGGAATGTTTCTATTCG+CGG | + | chr2.3:71831428-71831447 | None:intergenic | 45.0% |
| !! | TGAGGCAAGAGAGCATCAAA+AGG | - | chr2.3:71831897-71831916 | MS.gene01756:CDS | 45.0% |
| ACAGGGTAGGGGTCAGATTA+TGG | - | chr2.3:71831687-71831706 | MS.gene01756:CDS | 50.0% | |
| GCCGCGAATAGAAACATTCC+AGG | - | chr2.3:71831424-71831443 | MS.gene01756:CDS | 50.0% | |
| ! | GCAGCACTTGTTGAGTCACA+AGG | - | chr2.3:71831799-71831818 | MS.gene01756:CDS | 50.0% |
| AACATTCCAGGGTGTGGTCC+AGG | - | chr2.3:71831436-71831455 | MS.gene01756:CDS | 55.0% | |
| ! | GAAAGAGGAGCACGATGACG+AGG | + | chr2.3:71831966-71831985 | None:intergenic | 55.0% |
| GATGAACCTGGACCACACCC+TGG | + | chr2.3:71831445-71831464 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 71831109 | 71832023 | 71831109 | ID=MS.gene01756 |
| chr2.3 | mRNA | 71831109 | 71832023 | 71831109 | ID=MS.gene01756.t1;Parent=MS.gene01756 |
| chr2.3 | exon | 71831109 | 71832023 | 71831109 | ID=MS.gene01756.t1.exon1;Parent=MS.gene01756.t1 |
| chr2.3 | CDS | 71831109 | 71832023 | 71831109 | ID=cds.MS.gene01756.t1;Parent=MS.gene01756.t1 |
| Gene Sequence |
| Protein sequence |
