AlfalfaGEDB | MS.gene035217

M. sativa cultivar XinJiangDaYe / MS.gene035217

KEGG: K08486
GO: GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030674 GO:0033036 GO:0042886 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0060090 GO:0071702 GO:0071705 GO:0110165
PFAM: PF00804.28 PF05739.22

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035217.t1	XP_003608218.1	99	305	3	0	1	305	1	305	2.70E-130	474.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035217.t1	O64791	74.2	283	72	1	1	283	1	282	2.1e-110	400.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035217.t1	G7JRS4	99.0	305	3	0	1	305	1	305	2.0e-130	474.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene025786	MS.gene035217	PPI
MS.gene033077	MS.gene035217	PPI
MS.gene035217	MS.gene53170	PPI
MS.gene035217	MS.gene69311	PPI
MS.gene035217	MS.gene09947	PPI
MS.gene035217	MS.gene063476	PPI
MS.gene035217	MS.gene55383	PPI
MS.gene035217	MS.gene99692	PPI
MS.gene035217	MS.gene015460	PPI
MS.gene035217	MS.gene044431	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035217.t1	MTR_4g090900	99.016	305	3	0	1	305	1	305	0.0	622
MS.gene035217.t1	MTR_2g012270	88.816	304	33	1	1	304	1	303	0.0	563
MS.gene035217.t1	MTR_1g056550	58.741	286	109	3	1	278	1	285	1.06e-120	348
MS.gene035217.t1	MTR_1g056560	57.045	291	114	2	1	280	1	291	1.15e-118	345
MS.gene035217.t1	MTR_7g106420	55.000	280	120	1	1	280	1	274	9.79e-108	315
MS.gene035217.t1	MTR_5g012010	50.338	296	129	4	1	278	1	296	2.51e-99	295
MS.gene035217.t1	MTR_6g028140	48.239	284	139	2	1	280	1	280	9.00e-91	272
MS.gene035217.t1	MTR_2g088700	46.643	283	146	1	1	278	1	283	4.64e-87	263
MS.gene035217.t1	MTR_2g088700	43.522	301	165	1	1	296	1	301	5.18e-86	260
MS.gene035217.t1	MTR_5g020670	44.599	287	142	4	1	279	1	278	4.29e-72	224

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035217.t1	AT1G61290	77.632	304	63	2	1	304	1	299	1.62e-178	495
MS.gene035217.t1	AT1G61290	77.632	304	63	2	1	304	1	299	1.62e-178	495
MS.gene035217.t1	AT1G11250	80.357	280	49	2	1	280	1	274	3.87e-166	463
MS.gene035217.t1	AT4G03330	69.536	302	90	1	1	300	1	302	4.42e-149	421
MS.gene035217.t1	AT3G11820	67.194	253	83	0	26	278	5	257	3.34e-124	358
MS.gene035217.t1	AT3G11820	67.194	253	83	0	26	278	36	288	1.37e-123	357
MS.gene035217.t1	AT3G52400	55.973	293	112	3	1	280	1	289	6.99e-107	315
MS.gene035217.t1	AT3G03800	47.872	282	140	2	1	278	1	279	3.14e-88	266
MS.gene035217.t1	AT3G03800	47.872	282	140	2	1	278	1	279	3.14e-88	266
MS.gene035217.t1	AT1G08560	46.735	291	137	4	1	278	1	286	4.59e-86	261

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Find 43 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAATTGAACAATTGTAT+AGG	0.433871	4.2:+21512965	MS.gene035217:CDS
AAACGTGTCAAAATTATTAA+AGG	0.208067	4.2:+21513087	MS.gene035217:CDS
AAAGTGAAAATTTCATGCAA+AGG	0.560085	4.2:+21513367	MS.gene035217:CDS
AACAAAAGAATTCTAGGAAA+TGG	0.367316	4.2:+21513625	MS.gene035217:CDS
AACATTCCAGGGTGTGGTCC+AGG	0.464073	4.2:+21513150	MS.gene035217:CDS
AAGAGGGACAGAACAACTTC+AGG	0.421237	4.2:+21513590	MS.gene035217:CDS
AAGTGAAAATTTCATGCAAA+GGG	0.501636	4.2:+21513368	MS.gene035217:CDS
AATAGAAACATTCCAGGGTG+TGG	0.577755	4.2:+21513144	MS.gene035217:CDS
ACAAGGTAGGGGTCAAATAA+TGG	0.355079	4.2:+21513401	MS.gene035217:CDS
ACATGTTATGCCATAATTCT+TGG	0.318684	4.2:+21513648	MS.gene035217:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATGTTATGCCATAATTCT+TGG	+	chr4.2:21513648-21513667	MS.gene035217:CDS	30.0%
GAAGAAACTCAAAGATATGA+TGG	+	chr4.2:21513218-21513237	MS.gene035217:CDS	30.0%
ACTAGAACTTCTGTTGTTAG+TGG	+	chr4.2:21513189-21513208	MS.gene035217:CDS	35.0%
ACTTCTGTTGTTAGTGGTTT+AGG	+	chr4.2:21513195-21513214	MS.gene035217:CDS	35.0%
AGAAAGACATGATGCTGTTA+AGG	+	chr4.2:21513443-21513462	MS.gene035217:CDS	35.0%
CAGCTAATAGAAACATTCCA+GGG	+	chr4.2:21513139-21513158	MS.gene035217:CDS	35.0%
CGATAAATTCTTCGAAGATG+TGG	+	chr4.2:21512921-21512940	MS.gene035217:CDS	35.0%
CTTCTGTTGTTAGTGGTTTA+GGG	+	chr4.2:21513196-21513215	MS.gene035217:CDS	35.0%
GAGTCCAACAAAAGAATTCT+AGG	+	chr4.2:21513619-21513638	MS.gene035217:CDS	35.0%
GCATCTTGAGTATAAGGAAA+CGG	+	chr4.2:21513269-21513288	MS.gene035217:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	21512820	21513737	21512820	ID=MS.gene035217
chr4.2	mRNA	21512820	21513737	21512820	ID=MS.gene035217.t1;Parent=MS.gene035217
chr4.2	exon	21512820	21513737	21512820	ID=MS.gene035217.t1.exon1;Parent=MS.gene035217.t1
chr4.2	CDS	21512820	21513737	21512820	ID=cds.MS.gene035217.t1;Parent=MS.gene035217.t1

Gene Sequence

>MS.gene035217

ATGAACGACTTGTTCTCAAATTCCTTCAAAAAGTACACTGATTTGAAGGAACAAGCTTACCTTGATGATGTCGAGGCAGGGAATAGAGAAACTGTCAATCTCGATAAATTCTTCGAAGATGTGGAGAATATAAAAGAAGACATGAAAACAATTGAACAATTGTATAGGAAATTGAAAGAGGCTAATGAAGAAAGTAAAACCGTGCACAATGCTAAAACCATGAAAGAACTTCGAGCACGAATGGACAAAGATGTTGAACAAGTCCTTAAACGTGTCAAAATTATTAAAGGAAAACTCGAAGCATTGGATCGTTCAAATGCAGCTAATAGAAACATTCCAGGGTGTGGTCCAGGTTCATCAGCAGATAGAACTAGAACTTCTGTTGTTAGTGGTTTAGGGAAGAAACTCAAAGATATGATGGATGATTTTCAAGGTTTGAGAGCAAAAATGCATCTTGAGTATAAGGAAACGGTGGAACGGAGATATTTTACGATAACTGGAGAGAAAGCGGATGAAGAAACTATAGAAAATTTGATATCAAGTGGAGAAAGTGAAAATTTCATGCAAAGGGCAATTCAAGAACAAGGTAGGGGTCAAATAATGGACACTATTTCGGAAATTCAAGAAAGACATGATGCTGTTAAGGAAATAGAGAAGAATTTGATTGAGTTGCATCAAGTGTTTTTGGATATGGCAGCTTTGGTAGAGTCTCAAGGTCAACAACTTAATAATATTGAGAGTCATGTTGCACATGCTAGTTCTTTTGTAAGAAGAGGGACAGAACAACTTCAGGAAGCTAGAGTCCAACAAAAGAATTCTAGGAAATGGACATGTTATGCCATAATTCTTGGTATTGTTCTAGTTGTTGTGCTTTTGTTTCCTCTCTTGACATCACTTTTACCTCATTTGTTAAAATGA

Protein sequence

>MS.gene035217.t1

MNDLFSNSFKKYTDLKEQAYLDDVEAGNRETVNLDKFFEDVENIKEDMKTIEQLYRKLKEANEESKTVHNAKTMKELRARMDKDVEQVLKRVKIIKGKLEALDRSNAANRNIPGCGPGSSADRTRTSVVSGLGKKLKDMMDDFQGLRAKMHLEYKETVERRYFTITGEKADEETIENLISSGESENFMQRAIQEQGRGQIMDTISEIQERHDAVKEIEKNLIELHQVFLDMAALVESQGQQLNNIESHVAHASSFVRRGTEQLQEARVQQKNSRKWTCYAIILGIVLVVVLLFPLLTSLLPHLLK