Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035217.t1 | XP_003608218.1 | 99 | 305 | 3 | 0 | 1 | 305 | 1 | 305 | 2.70E-130 | 474.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035217.t1 | O64791 | 74.2 | 283 | 72 | 1 | 1 | 283 | 1 | 282 | 2.1e-110 | 400.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035217.t1 | G7JRS4 | 99.0 | 305 | 3 | 0 | 1 | 305 | 1 | 305 | 2.0e-130 | 474.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035217 | MS.gene53170 | PPI |
| MS.gene025786 | MS.gene035217 | PPI |
| MS.gene70948 | MS.gene035217 | PPI |
| MS.gene033077 | MS.gene035217 | PPI |
| MS.gene21144 | MS.gene035217 | PPI |
| MS.gene035217 | MS.gene69311 | PPI |
| MS.gene035217 | MS.gene09947 | PPI |
| MS.gene035217 | MS.gene063476 | PPI |
| MS.gene035781 | MS.gene035217 | PPI |
| MS.gene035217 | MS.gene55383 | PPI |
| MS.gene035217 | MS.gene99692 | PPI |
| MS.gene73141 | MS.gene035217 | PPI |
| MS.gene035217 | MS.gene015460 | PPI |
| MS.gene064565 | MS.gene035217 | PPI |
| MS.gene035217 | MS.gene044431 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035217.t1 | MTR_4g090900 | 99.016 | 305 | 3 | 0 | 1 | 305 | 1 | 305 | 0.0 | 622 |
| MS.gene035217.t1 | MTR_2g012270 | 88.816 | 304 | 33 | 1 | 1 | 304 | 1 | 303 | 0.0 | 563 |
| MS.gene035217.t1 | MTR_1g056550 | 58.741 | 286 | 109 | 3 | 1 | 278 | 1 | 285 | 1.06e-120 | 348 |
| MS.gene035217.t1 | MTR_1g056560 | 57.045 | 291 | 114 | 2 | 1 | 280 | 1 | 291 | 1.15e-118 | 345 |
| MS.gene035217.t1 | MTR_7g106420 | 55.000 | 280 | 120 | 1 | 1 | 280 | 1 | 274 | 9.79e-108 | 315 |
| MS.gene035217.t1 | MTR_5g012010 | 50.338 | 296 | 129 | 4 | 1 | 278 | 1 | 296 | 2.51e-99 | 295 |
| MS.gene035217.t1 | MTR_6g028140 | 48.239 | 284 | 139 | 2 | 1 | 280 | 1 | 280 | 9.00e-91 | 272 |
| MS.gene035217.t1 | MTR_2g088700 | 46.643 | 283 | 146 | 1 | 1 | 278 | 1 | 283 | 4.64e-87 | 263 |
| MS.gene035217.t1 | MTR_2g088700 | 43.522 | 301 | 165 | 1 | 1 | 296 | 1 | 301 | 5.18e-86 | 260 |
| MS.gene035217.t1 | MTR_5g020670 | 44.599 | 287 | 142 | 4 | 1 | 279 | 1 | 278 | 4.29e-72 | 224 |
| MS.gene035217.t1 | MTR_8g073900 | 40.476 | 168 | 71 | 3 | 1 | 161 | 1 | 146 | 1.39e-25 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035217.t1 | AT1G61290 | 77.632 | 304 | 63 | 2 | 1 | 304 | 1 | 299 | 1.62e-178 | 495 |
| MS.gene035217.t1 | AT1G61290 | 77.632 | 304 | 63 | 2 | 1 | 304 | 1 | 299 | 1.62e-178 | 495 |
| MS.gene035217.t1 | AT1G11250 | 80.357 | 280 | 49 | 2 | 1 | 280 | 1 | 274 | 3.87e-166 | 463 |
| MS.gene035217.t1 | AT4G03330 | 69.536 | 302 | 90 | 1 | 1 | 300 | 1 | 302 | 4.42e-149 | 421 |
| MS.gene035217.t1 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 5 | 257 | 3.34e-124 | 358 |
| MS.gene035217.t1 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 36 | 288 | 1.37e-123 | 357 |
| MS.gene035217.t1 | AT3G52400 | 55.973 | 293 | 112 | 3 | 1 | 280 | 1 | 289 | 6.99e-107 | 315 |
| MS.gene035217.t1 | AT3G03800 | 47.872 | 282 | 140 | 2 | 1 | 278 | 1 | 279 | 3.14e-88 | 266 |
| MS.gene035217.t1 | AT3G03800 | 47.872 | 282 | 140 | 2 | 1 | 278 | 1 | 279 | 3.14e-88 | 266 |
| MS.gene035217.t1 | AT1G08560 | 46.735 | 291 | 137 | 4 | 1 | 278 | 1 | 286 | 4.59e-86 | 261 |
| MS.gene035217.t1 | AT5G08080 | 49.643 | 280 | 137 | 2 | 1 | 278 | 1 | 278 | 1.92e-81 | 249 |
| MS.gene035217.t1 | AT5G08080 | 49.643 | 280 | 137 | 2 | 1 | 278 | 1 | 278 | 1.92e-81 | 249 |
| MS.gene035217.t1 | AT5G08080 | 47.423 | 291 | 138 | 4 | 1 | 278 | 1 | 289 | 2.58e-79 | 244 |
| MS.gene035217.t1 | AT2G18260 | 36.977 | 311 | 176 | 5 | 1 | 298 | 1 | 304 | 1.26e-63 | 203 |
| MS.gene035217.t1 | AT2G18260 | 36.977 | 311 | 176 | 5 | 1 | 298 | 1 | 304 | 1.26e-63 | 203 |
| MS.gene035217.t1 | AT5G08080 | 46.040 | 202 | 105 | 2 | 1 | 200 | 1 | 200 | 3.10e-44 | 150 |
| MS.gene035217.t1 | AT5G08080 | 46.040 | 202 | 105 | 2 | 1 | 200 | 1 | 200 | 3.10e-44 | 150 |
Find 43 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCTGTTGTTAGTGGTTT+AGG | 0.186018 | 4.2:+21513195 | MS.gene035217:CDS |
| AAACGTGTCAAAATTATTAA+AGG | 0.208067 | 4.2:+21513087 | MS.gene035217:CDS |
| GAGTCCAACAAAAGAATTCT+AGG | 0.285437 | 4.2:+21513619 | MS.gene035217:CDS |
| CTTCTGTTGTTAGTGGTTTA+GGG | 0.303686 | 4.2:+21513196 | MS.gene035217:CDS |
| TCAAATAATGGACACTATTT+CGG | 0.305761 | 4.2:+21513413 | MS.gene035217:CDS |
| TCGTGCTCGAAGTTCTTTCA+TGG | 0.315609 | 4.2:-21513037 | None:intergenic |
| ACATGTTATGCCATAATTCT+TGG | 0.318684 | 4.2:+21513648 | MS.gene035217:CDS |
| TAGAACAATACCAAGAATTA+TGG | 0.331526 | 4.2:-21513658 | None:intergenic |
| ACAAGGTAGGGGTCAAATAA+TGG | 0.355079 | 4.2:+21513401 | MS.gene035217:CDS |
| AACAAAAGAATTCTAGGAAA+TGG | 0.367316 | 4.2:+21513625 | MS.gene035217:CDS |
| ACTAGAACTTCTGTTGTTAG+TGG | 0.379314 | 4.2:+21513189 | MS.gene035217:CDS |
| AGAAAGACATGATGCTGTTA+AGG | 0.392149 | 4.2:+21513443 | MS.gene035217:CDS |
| TACCTCATTTGTTAAAATGA+AGG | 0.394311 | 4.2:+21513718 | MS.gene035217:CDS |
| AAGAGGGACAGAACAACTTC+AGG | 0.421237 | 4.2:+21513590 | MS.gene035217:CDS |
| AAACAATTGAACAATTGTAT+AGG | 0.433871 | 4.2:+21512965 | MS.gene035217:CDS |
| TACCTTGATGATGTCGAGGC+AGG | 0.443621 | 4.2:+21512877 | MS.gene035217:CDS |
| GCAGCTAATAGAAACATTCC+AGG | 0.458362 | 4.2:+21513138 | MS.gene035217:CDS |
| TAAAGGAAAACTCGAAGCAT+TGG | 0.462694 | 4.2:+21513104 | MS.gene035217:CDS |
| AACATTCCAGGGTGTGGTCC+AGG | 0.464073 | 4.2:+21513150 | MS.gene035217:CDS |
| GAAGAAACTCAAAGATATGA+TGG | 0.479536 | 4.2:+21513218 | MS.gene035217:CDS |
| GTTCTATCTGCTGATGAACC+TGG | 0.498169 | 4.2:-21513168 | None:intergenic |
| AAGTGAAAATTTCATGCAAA+GGG | 0.501636 | 4.2:+21513368 | MS.gene035217:CDS |
| GGCAATTCAAGAACAAGGTA+GGG | 0.504105 | 4.2:+21513389 | MS.gene035217:CDS |
| GGGCAATTCAAGAACAAGGT+AGG | 0.529449 | 4.2:+21513388 | MS.gene035217:CDS |
| ATAGAAAATTTGATATCAAG+TGG | 0.532572 | 4.2:+21513342 | MS.gene035217:CDS |
| ACCTTGATGATGTCGAGGCA+GGG | 0.538277 | 4.2:+21512878 | MS.gene035217:CDS |
| GAAAGAACTTCGAGCACGAA+TGG | 0.541312 | 4.2:+21513041 | MS.gene035217:CDS |
| GCATCTTGAGTATAAGGAAA+CGG | 0.542829 | 4.2:+21513269 | MS.gene035217:CDS |
| CGATAAATTCTTCGAAGATG+TGG | 0.548904 | 4.2:+21512921 | MS.gene035217:CDS |
| ATTGTATAGGAAATTGAAAG+AGG | 0.552639 | 4.2:+21512978 | MS.gene035217:CDS |
| GATGAACCTGGACCACACCC+TGG | 0.554068 | 4.2:-21513156 | None:intergenic |
| AAAGTGAAAATTTCATGCAA+AGG | 0.560085 | 4.2:+21513367 | MS.gene035217:CDS |
| AATAGAAACATTCCAGGGTG+TGG | 0.577755 | 4.2:+21513144 | MS.gene035217:CDS |
| TACGATAACTGGAGAGAAAG+CGG | 0.583135 | 4.2:+21513308 | MS.gene035217:CDS |
| GGTAAAAGTGATGTCAAGAG+AGG | 0.586377 | 4.2:-21513699 | None:intergenic |
| TCCCTGCCTCGACATCATCA+AGG | 0.592333 | 4.2:-21512879 | None:intergenic |
| CAAAGGGCAATTCAAGAACA+AGG | 0.597990 | 4.2:+21513384 | MS.gene035217:CDS |
| AGTATAAGGAAACGGTGGAA+CGG | 0.624743 | 4.2:+21513277 | MS.gene035217:CDS |
| AGCTTACCTTGATGATGTCG+AGG | 0.625446 | 4.2:+21512873 | MS.gene035217:CDS |
| GCAATTCAAGAACAAGGTAG+GGG | 0.626558 | 4.2:+21513390 | MS.gene035217:CDS |
| GCAGCTTTGGTAGAGTCTCA+AGG | 0.638480 | 4.2:+21513513 | MS.gene035217:CDS |
| CAGCTAATAGAAACATTCCA+GGG | 0.687559 | 4.2:+21513139 | MS.gene035217:CDS |
| TCTTGAGTATAAGGAAACGG+TGG | 0.715657 | 4.2:+21513272 | MS.gene035217:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAATTGAACAATTGTAT+AGG | + | chr4.2:21512965-21512984 | MS.gene035217:CDS | 20.0% |
| !! | AAACGTGTCAAAATTATTAA+AGG | + | chr4.2:21513087-21513106 | MS.gene035217:CDS | 20.0% |
| !! | ATAGAAAATTTGATATCAAG+TGG | + | chr4.2:21513342-21513361 | MS.gene035217:CDS | 20.0% |
| !!! | TAATAATTTTGACACGTTTA+AGG | - | chr4.2:21513086-21513105 | None:intergenic | 20.0% |
| ! | AAAAATGCATCTTGAGTATA+AGG | + | chr4.2:21513263-21513282 | MS.gene035217:CDS | 25.0% |
| ! | AAAGTGAAAATTTCATGCAA+AGG | + | chr4.2:21513367-21513386 | MS.gene035217:CDS | 25.0% |
| ! | AAGTGAAAATTTCATGCAAA+GGG | + | chr4.2:21513368-21513387 | MS.gene035217:CDS | 25.0% |
| ! | ATTGTATAGGAAATTGAAAG+AGG | + | chr4.2:21512978-21512997 | MS.gene035217:CDS | 25.0% |
| ! | TAGAACAATACCAAGAATTA+TGG | - | chr4.2:21513661-21513680 | None:intergenic | 25.0% |
| !! | AACAAAAGAATTCTAGGAAA+TGG | + | chr4.2:21513625-21513644 | MS.gene035217:CDS | 25.0% |
| !! | ATTTCCTAGAATTCTTTTGT+TGG | - | chr4.2:21513626-21513645 | None:intergenic | 25.0% |
| !!! | TCAAATAATGGACACTATTT+CGG | + | chr4.2:21513413-21513432 | MS.gene035217:CDS | 25.0% |
| ACATGTTATGCCATAATTCT+TGG | + | chr4.2:21513648-21513667 | MS.gene035217:CDS | 30.0% | |
| GAAGAAACTCAAAGATATGA+TGG | + | chr4.2:21513218-21513237 | MS.gene035217:CDS | 30.0% | |
| !! | CAAAAAGTACACTGATTTGA+AGG | + | chr4.2:21512846-21512865 | MS.gene035217:CDS | 30.0% |
| !! | CAAATCAGTGTACTTTTTGA+AGG | - | chr4.2:21512845-21512864 | None:intergenic | 30.0% |
| !! | CTAGTTCTTTTGTAAGAAGA+GGG | + | chr4.2:21513574-21513593 | MS.gene035217:CDS | 30.0% |
| !!! | GATATGATGGATGATTTTCA+AGG | + | chr4.2:21513231-21513250 | MS.gene035217:CDS | 30.0% |
| ACTAGAACTTCTGTTGTTAG+TGG | + | chr4.2:21513189-21513208 | MS.gene035217:CDS | 35.0% | |
| ACTTCTGTTGTTAGTGGTTT+AGG | + | chr4.2:21513195-21513214 | MS.gene035217:CDS | 35.0% | |
| AGAAAGACATGATGCTGTTA+AGG | + | chr4.2:21513443-21513462 | MS.gene035217:CDS | 35.0% | |
| CAGCTAATAGAAACATTCCA+GGG | + | chr4.2:21513139-21513158 | MS.gene035217:CDS | 35.0% | |
| CGATAAATTCTTCGAAGATG+TGG | + | chr4.2:21512921-21512940 | MS.gene035217:CDS | 35.0% | |
| CTTCTGTTGTTAGTGGTTTA+GGG | + | chr4.2:21513196-21513215 | MS.gene035217:CDS | 35.0% | |
| GAGTCCAACAAAAGAATTCT+AGG | + | chr4.2:21513619-21513638 | MS.gene035217:CDS | 35.0% | |
| GCATCTTGAGTATAAGGAAA+CGG | + | chr4.2:21513269-21513288 | MS.gene035217:CDS | 35.0% | |
| TAAAGGAAAACTCGAAGCAT+TGG | + | chr4.2:21513104-21513123 | MS.gene035217:CDS | 35.0% | |
| ! | CGGAGATATTTTACGATAAC+TGG | + | chr4.2:21513297-21513316 | MS.gene035217:CDS | 35.0% |
| ! | TGAGTTGCATCAAGTGTTTT+TGG | + | chr4.2:21513485-21513504 | MS.gene035217:CDS | 35.0% |
| !! | GCTAGTTCTTTTGTAAGAAG+AGG | + | chr4.2:21513573-21513592 | MS.gene035217:CDS | 35.0% |
| !!! | GCATCAAGTGTTTTTGGATA+TGG | + | chr4.2:21513491-21513510 | MS.gene035217:CDS | 35.0% |
| AATAGAAACATTCCAGGGTG+TGG | + | chr4.2:21513144-21513163 | MS.gene035217:CDS | 40.0% | |
| ACAAGGTAGGGGTCAAATAA+TGG | + | chr4.2:21513401-21513420 | MS.gene035217:CDS | 40.0% | |
| AGTATAAGGAAACGGTGGAA+CGG | + | chr4.2:21513277-21513296 | MS.gene035217:CDS | 40.0% | |
| CAAAGGGCAATTCAAGAACA+AGG | + | chr4.2:21513384-21513403 | MS.gene035217:CDS | 40.0% | |
| GCAATTCAAGAACAAGGTAG+GGG | + | chr4.2:21513390-21513409 | MS.gene035217:CDS | 40.0% | |
| GCAGCTAATAGAAACATTCC+AGG | + | chr4.2:21513138-21513157 | MS.gene035217:CDS | 40.0% | |
| GGCAATTCAAGAACAAGGTA+GGG | + | chr4.2:21513389-21513408 | MS.gene035217:CDS | 40.0% | |
| GGTAAAAGTGATGTCAAGAG+AGG | - | chr4.2:21513702-21513721 | None:intergenic | 40.0% | |
| TACGATAACTGGAGAGAAAG+CGG | + | chr4.2:21513308-21513327 | MS.gene035217:CDS | 40.0% | |
| TCTTGAGTATAAGGAAACGG+TGG | + | chr4.2:21513272-21513291 | MS.gene035217:CDS | 40.0% | |
| !! | GTTTTTGGATATGGCAGCTT+TGG | + | chr4.2:21513500-21513519 | MS.gene035217:CDS | 40.0% |
| !!! | CATGGTTTTAGCATTGTGCA+CGG | - | chr4.2:21513022-21513041 | None:intergenic | 40.0% |
| AAGAGGGACAGAACAACTTC+AGG | + | chr4.2:21513590-21513609 | MS.gene035217:CDS | 45.0% | |
| GAAAGAACTTCGAGCACGAA+TGG | + | chr4.2:21513041-21513060 | MS.gene035217:CDS | 45.0% | |
| GGGCAATTCAAGAACAAGGT+AGG | + | chr4.2:21513388-21513407 | MS.gene035217:CDS | 45.0% | |
| GTTCTATCTGCTGATGAACC+TGG | - | chr4.2:21513171-21513190 | None:intergenic | 45.0% | |
| TCGTGCTCGAAGTTCTTTCA+TGG | - | chr4.2:21513040-21513059 | None:intergenic | 45.0% | |
| !! | AGCTTACCTTGATGATGTCG+AGG | + | chr4.2:21512873-21512892 | MS.gene035217:CDS | 45.0% |
| ! | GCAGCTTTGGTAGAGTCTCA+AGG | + | chr4.2:21513513-21513532 | MS.gene035217:CDS | 50.0% |
| !! | ACCTTGATGATGTCGAGGCA+GGG | + | chr4.2:21512878-21512897 | MS.gene035217:CDS | 50.0% |
| !! | TACCTTGATGATGTCGAGGC+AGG | + | chr4.2:21512877-21512896 | MS.gene035217:CDS | 50.0% |
| AACATTCCAGGGTGTGGTCC+AGG | + | chr4.2:21513150-21513169 | MS.gene035217:CDS | 55.0% | |
| TCCCTGCCTCGACATCATCA+AGG | - | chr4.2:21512882-21512901 | None:intergenic | 55.0% | |
| GATGAACCTGGACCACACCC+TGG | - | chr4.2:21513159-21513178 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 21512820 | 21513737 | 21512820 | ID=MS.gene035217 |
| chr4.2 | mRNA | 21512820 | 21513737 | 21512820 | ID=MS.gene035217.t1;Parent=MS.gene035217 |
| chr4.2 | exon | 21512820 | 21513737 | 21512820 | ID=MS.gene035217.t1.exon1;Parent=MS.gene035217.t1 |
| chr4.2 | CDS | 21512820 | 21513737 | 21512820 | ID=cds.MS.gene035217.t1;Parent=MS.gene035217.t1 |
| Gene Sequence |
| Protein sequence |
