AlfalfaGEDB | MS.gene01347

M. sativa cultivar XinJiangDaYe / MS.gene01347

KEGG: K08486
GO: GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030674 GO:0033036 GO:0042886 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0060090 GO:0071702 GO:0071705 GO:0110165
PFAM: PF00804.28 PF05739.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01347.t1	XP_024632050.1	99.7	304	1	0	1	304	1	304	2.10E-151	545

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01347.t1	O64791	80.9	282	54	0	1	282	1	282	1.2e-123	444.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01347.t1	G7ILD5	99.7	304	1	0	1	304	1	304	1.5e-151	545.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene73141	MS.gene01347	PPI
MS.gene015460	MS.gene01347	PPI
MS.gene064565	MS.gene01347	PPI
MS.gene025786	MS.gene01347	PPI
MS.gene70948	MS.gene01347	PPI
MS.gene033077	MS.gene01347	PPI
MS.gene21144	MS.gene01347	PPI
MS.gene01347	MS.gene09947	PPI
MS.gene063476	MS.gene01347	PPI
MS.gene53170	MS.gene01347	PPI
MS.gene044431	MS.gene01347	PPI
MS.gene69311	MS.gene01347	PPI
MS.gene035781	MS.gene01347	PPI
MS.gene01347	MS.gene55383	PPI
MS.gene01347	MS.gene99692	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01347.t1	MTR_2g012270	99.671	304	1	0	1	304	1	304	0.0	622
MS.gene01347.t1	MTR_4g090900	90.000	280	27	1	1	279	1	280	0.0	525
MS.gene01347.t1	MTR_1g056550	58.042	286	110	2	1	277	1	285	6.13e-122	352
MS.gene01347.t1	MTR_1g056560	56.701	291	114	1	1	279	1	291	4.61e-119	345
MS.gene01347.t1	MTR_7g106420	57.490	247	105	0	33	279	28	274	3.20e-105	309
MS.gene01347.t1	MTR_5g012010	49.324	296	131	4	1	277	1	296	7.76e-99	293
MS.gene01347.t1	MTR_2g088700	44.444	306	161	3	1	300	1	303	2.52e-87	264
MS.gene01347.t1	MTR_6g028140	46.953	279	145	2	1	277	1	278	2.24e-86	261
MS.gene01347.t1	MTR_2g088700	45.936	283	147	1	1	277	1	283	6.28e-86	260
MS.gene01347.t1	MTR_5g020670	43.206	287	145	4	1	278	1	278	3.75e-70	219
MS.gene01347.t1	MTR_8g073900	39.286	168	72	3	1	160	1	146	2.35e-23	94.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01347.t1	AT1G61290	77.961	304	63	1	1	304	1	300	6.06e-180	498
MS.gene01347.t1	AT1G61290	77.961	304	63	1	1	304	1	300	6.06e-180	498
MS.gene01347.t1	AT1G11250	79.211	279	53	1	1	279	1	274	4.53e-166	463
MS.gene01347.t1	AT4G03330	68.543	302	92	1	1	299	1	302	1.16e-146	414
MS.gene01347.t1	AT3G11820	65.992	247	84	0	31	277	11	257	9.78e-120	347
MS.gene01347.t1	AT3G11820	63.566	258	94	0	20	277	31	288	2.19e-119	347
MS.gene01347.t1	AT3G52400	54.671	289	121	2	1	279	1	289	3.56e-105	310
MS.gene01347.t1	AT3G03800	47.872	282	139	2	1	277	1	279	2.49e-88	266
MS.gene01347.t1	AT3G03800	47.872	282	139	2	1	277	1	279	2.49e-88	266
MS.gene01347.t1	AT1G08560	46.048	291	138	4	1	277	1	286	3.17e-85	258
MS.gene01347.t1	AT5G08080	49.643	280	136	2	1	277	1	278	1.14e-80	246
MS.gene01347.t1	AT5G08080	49.643	280	136	2	1	277	1	278	1.14e-80	246
MS.gene01347.t1	AT5G08080	46.735	291	139	4	1	277	1	289	1.75e-77	239
MS.gene01347.t1	AT2G18260	36.656	311	176	5	1	297	1	304	2.31e-64	205
MS.gene01347.t1	AT2G18260	36.656	311	176	5	1	297	1	304	2.31e-64	205
MS.gene01347.t1	AT5G08080	47.030	202	102	2	1	199	1	200	7.09e-45	152
MS.gene01347.t1	AT5G08080	47.030	202	102	2	1	199	1	200	7.09e-45	152

Find 39 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGCTTCTACATCATCAATT+TGG	0.171423	2.2:+70036343	None:intergenic
CAAAAGAGTCAAAATCATTA+AGG	0.241294	2.2:-70036136	MS.gene01347:CDS
ACAAGGTAGGGGTCAGATTA+TGG	0.287097	2.2:-70035821	MS.gene01347:CDS
TGCTTCTACATCATCAATTT+GGG	0.291794	2.2:+70036344	None:intergenic
CCCTGGAATGTTTCTATTTG+CGG	0.343881	2.2:+70036083	None:intergenic
CAACCTTGATAAATTCTTTG+AGG	0.373925	2.2:-70036307	MS.gene01347:CDS
ACTTCAGTGGTTAGTGGATT+GGG	0.377345	2.2:-70036027	MS.gene01347:CDS
AACTTCAGTGGTTAGTGGAT+TGG	0.416019	2.2:-70036028	MS.gene01347:CDS
ATCAAAAGGATTCAAGGAAA+TGG	0.420191	2.2:-70035597	MS.gene01347:CDS
GATGGATAAAGATGTTGAAC+AGG	0.436094	2.2:-70036163	MS.gene01347:CDS
AAAAGAGTCAAAATCATTAA+GGG	0.451614	2.2:-70036135	MS.gene01347:CDS
AACATTCCAGGGTGTGGTCC+AGG	0.464073	2.2:-70036072	MS.gene01347:CDS
GTTCTATCTGCTGATGAACC+TGG	0.467339	2.2:+70036054	None:intergenic
GCCGCAAATAGAAACATTCC+AGG	0.468166	2.2:-70036084	MS.gene01347:CDS
GAGAGCATCAAAAGGATTCA+AGG	0.474516	2.2:-70035603	MS.gene01347:CDS
ACAAGAACTTCAGTGGTTAG+TGG	0.481738	2.2:-70036033	MS.gene01347:CDS
ATTGAGAATTTGATATCAAG+TGG	0.510431	2.2:-70035880	MS.gene01347:CDS
CATCCTCAAAGAATTTATCA+AGG	0.518396	2.2:+70036304	None:intergenic
CAAATTGATGATGTAGAAGC+AGG	0.522039	2.2:-70036342	MS.gene01347:CDS
AGCAATTCAAGAACAAGGTA+GGG	0.529179	2.2:-70035833	MS.gene01347:CDS
GAGCAATTCAAGAACAAGGT+AGG	0.536020	2.2:-70035834	MS.gene01347:CDS
CAATGAAAGATCTTAGATCA+AGG	0.542118	2.2:-70036185	MS.gene01347:CDS
GAAAGATCTTAGATCAAGGA+TGG	0.545537	2.2:-70036181	MS.gene01347:CDS
ACAAAGAAACAATTGAAAGA+AGG	0.546398	2.2:-70035942	MS.gene01347:CDS
GATGAACCTGGACCACACCC+TGG	0.554068	2.2:+70036066	None:intergenic
TGAGGCAAGAGAGCATCAAA+AGG	0.557741	2.2:-70035611	MS.gene01347:CDS
TTGACATCAACAATGGAAAG+AGG	0.573552	2.2:+70035530	None:intergenic
AATAGAAACATTCCAGGGTG+TGG	0.577755	2.2:-70036078	MS.gene01347:CDS
CAAAGAGCAATTCAAGAACA+AGG	0.592503	2.2:-70035838	MS.gene01347:CDS
GAAAGAGGAGCACGATGACG+AGG	0.604139	2.2:+70035545	None:intergenic
GCTCTATAGAAAATTACAAG+AGG	0.608759	2.2:-70036244	MS.gene01347:CDS
GGTAGAATTGACATCAACAA+TGG	0.610196	2.2:+70035523	None:intergenic
GCAATTCAAGAACAAGGTAG+GGG	0.620511	2.2:-70035832	MS.gene01347:CDS
AGAATGTGAAAGAAGACATG+AGG	0.630310	2.2:-70036278	MS.gene01347:CDS
AGATAGAACAAGAACTTCAG+TGG	0.645922	2.2:-70036040	MS.gene01347:CDS
AGGTACTGAACAACTTCATG+AGG	0.661031	2.2:-70035629	MS.gene01347:CDS
GCAGCACTTGTTGAGTCACA+AGG	0.663060	2.2:-70035709	MS.gene01347:CDS
CCGCAAATAGAAACATTCCA+GGG	0.686589	2.2:-70036083	MS.gene01347:CDS
TAATTCTACAGAAACAAGTG+AGG	0.702509	2.2:+70035502	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAACTCAAAGATATGA+TGG	-	chr2.2:70035902-70035921	MS.gene01347:CDS	20.0%
!!	AAAAGAGTCAAAATCATTAA+GGG	-	chr2.2:70035771-70035790	MS.gene01347:CDS	20.0%
!	ACAAAGAAACAATTGAAAGA+AGG	-	chr2.2:70035964-70035983	MS.gene01347:CDS	25.0%
!	ATTGAGAATTTGATATCAAG+TGG	-	chr2.2:70036026-70036045	MS.gene01347:CDS	25.0%
!	CAAAAGAGTCAAAATCATTA+AGG	-	chr2.2:70035770-70035789	MS.gene01347:CDS	25.0%
!!!	AGAAGGTATTTTACAATAAC+TGG	-	chr2.2:70035981-70036000	MS.gene01347:CDS	25.0%
!!!	ATATGATGGATGATTTTCAA+GGG	-	chr2.2:70035916-70035935	MS.gene01347:CDS	25.0%
	ATCAAAAGGATTCAAGGAAA+TGG	-	chr2.2:70036309-70036328	MS.gene01347:CDS	30.0%
	CAAAAAATACAGTGACTTGA+AGG	-	chr2.2:70035533-70035552	MS.gene01347:CDS	30.0%
	CAACCTTGATAAATTCTTTG+AGG	-	chr2.2:70035599-70035618	MS.gene01347:CDS	30.0%
	CAATGAAAGATCTTAGATCA+AGG	-	chr2.2:70035721-70035740	MS.gene01347:CDS	30.0%
	CATCCTCAAAGAATTTATCA+AGG	+	chr2.2:70035605-70035624	None:intergenic	30.0%
	GCTCTATAGAAAATTACAAG+AGG	-	chr2.2:70035662-70035681	MS.gene01347:CDS	30.0%
	TGCTTCTACATCATCAATTT+GGG	+	chr2.2:70035565-70035584	None:intergenic	30.0%
!	TGAGTTGCATCAAGTATTTT+TGG	-	chr2.2:70036169-70036188	MS.gene01347:CDS	30.0%
!!!	CAAGTCACTGTATTTTTTGA+AGG	+	chr2.2:70035532-70035551	None:intergenic	30.0%
!!!	CTTTCATTGTTTTAGCATTG+TGG	+	chr2.2:70035710-70035729	None:intergenic	30.0%
!!!	GATATGATGGATGATTTTCA+AGG	-	chr2.2:70035915-70035934	MS.gene01347:CDS	30.0%
	AGAATGTGAAAGAAGACATG+AGG	-	chr2.2:70035628-70035647	MS.gene01347:CDS	35.0%
	AGATAGAACAAGAACTTCAG+TGG	-	chr2.2:70035866-70035885	MS.gene01347:CDS	35.0%
	AGCAATTCAAGAACAAGGTA+GGG	-	chr2.2:70036073-70036092	MS.gene01347:CDS	35.0%
	CAAAGAGCAATTCAAGAACA+AGG	-	chr2.2:70036068-70036087	MS.gene01347:CDS	35.0%
	CTGCTTCTACATCATCAATT+TGG	+	chr2.2:70035566-70035585	None:intergenic	35.0%
	GAAAGATCTTAGATCAAGGA+TGG	-	chr2.2:70035725-70035744	MS.gene01347:CDS	35.0%
	GATGGATAAAGATGTTGAAC+AGG	-	chr2.2:70035743-70035762	MS.gene01347:CDS	35.0%
	GGTAGAATTGACATCAACAA+TGG	+	chr2.2:70036386-70036405	None:intergenic	35.0%
	TTGACATCAACAATGGAAAG+AGG	+	chr2.2:70036379-70036398	None:intergenic	35.0%
!!	CAAATTGATGATGTAGAAGC+AGG	-	chr2.2:70035564-70035583	MS.gene01347:CDS	35.0%
!!	GCTAGTTCTTTTGTTAGAAG+AGG	-	chr2.2:70036257-70036276	MS.gene01347:CDS	35.0%
!!!	GCATCAAGTATTTTTGGACA+TGG	-	chr2.2:70036175-70036194	MS.gene01347:CDS	35.0%
	AATAGAAACATTCCAGGGTG+TGG	-	chr2.2:70035828-70035847	MS.gene01347:CDS	40.0%
	ACAAGAACTTCAGTGGTTAG+TGG	-	chr2.2:70035873-70035892	MS.gene01347:CDS	40.0%
	CCGCAAATAGAAACATTCCA+GGG	-	chr2.2:70035823-70035842	MS.gene01347:CDS	40.0%
	GAGCAATTCAAGAACAAGGT+AGG	-	chr2.2:70036072-70036091	MS.gene01347:CDS	40.0%
	GCAATTCAAGAACAAGGTAG+GGG	-	chr2.2:70036074-70036093	MS.gene01347:CDS	40.0%
!	AACTTCAGTGGTTAGTGGAT+TGG	-	chr2.2:70035878-70035897	MS.gene01347:CDS	40.0%
!	CCCTGGAATGTTTCTATTTG+CGG	+	chr2.2:70035826-70035845	None:intergenic	40.0%
!!	ACTTCAGTGGTTAGTGGATT+GGG	-	chr2.2:70035879-70035898	MS.gene01347:CDS	40.0%
!!	AGGTACTGAACAACTTCATG+AGG	-	chr2.2:70036277-70036296	MS.gene01347:CDS	40.0%
!!	GAGAGCATCAAAAGGATTCA+AGG	-	chr2.2:70036303-70036322	MS.gene01347:CDS	40.0%
	ACAAGGTAGGGGTCAGATTA+TGG	-	chr2.2:70036085-70036104	MS.gene01347:CDS	45.0%
	GCCGCAAATAGAAACATTCC+AGG	-	chr2.2:70035822-70035841	MS.gene01347:CDS	45.0%
	GTTCTATCTGCTGATGAACC+TGG	+	chr2.2:70035855-70035874	None:intergenic	45.0%
!!	TGAGGCAAGAGAGCATCAAA+AGG	-	chr2.2:70036295-70036314	MS.gene01347:CDS	45.0%
!	GCAGCACTTGTTGAGTCACA+AGG	-	chr2.2:70036197-70036216	MS.gene01347:CDS	50.0%
	AACATTCCAGGGTGTGGTCC+AGG	-	chr2.2:70035834-70035853	MS.gene01347:CDS	55.0%
!	GAAAGAGGAGCACGATGACG+AGG	+	chr2.2:70036364-70036383	None:intergenic	55.0%
	GATGAACCTGGACCACACCC+TGG	+	chr2.2:70035843-70035862	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	70035507	70036421	70035507	ID=MS.gene01347
chr2.2	mRNA	70035507	70036421	70035507	ID=MS.gene01347.t1;Parent=MS.gene01347
chr2.2	exon	70035507	70036421	70035507	ID=MS.gene01347.t1.exon1;Parent=MS.gene01347.t1
chr2.2	CDS	70035507	70036421	70035507	ID=cds.MS.gene01347.t1;Parent=MS.gene01347.t1

Gene Sequence

>MS.gene01347

ATGAATGACTTATTCTCAAGCTCCTTCAAAAAATACAGTGACTTGAAGGAGCAAGCCCAAATTGATGATGTAGAAGCAGGAAAAGAGAGTATCAACCTTGATAAATTCTTTGAGGATGTTGAGAATGTGAAAGAAGACATGAGGCTTGTTGAGAAGCTCTATAGAAAATTACAAGAGGCAAATGAAGAAAGCAAAATTGTCCACAATGCTAAAACAATGAAAGATCTTAGATCAAGGATGGATAAAGATGTTGAACAGGTTCTCAAAAGAGTCAAAATCATTAAGGGTAAGCTTGAAGCATTAGAACGTTCGAATGCCGCAAATAGAAACATTCCAGGGTGTGGTCCAGGTTCATCAGCAGATAGAACAAGAACTTCAGTGGTTAGTGGATTGGGAAAAAAACTCAAAGATATGATGGATGATTTTCAAGGGTTAAGAGCAAGAATGCAACAAGAGTACAAAGAAACAATTGAAAGAAGGTATTTTACAATAACTGGTGAGAAAGCTGATGAAGATACTATTGAGAATTTGATATCAAGTGGAGAAAGTGAAACTTTTATGCAAAGAGCAATTCAAGAACAAGGTAGGGGTCAGATTATGGACACAATATCTGAGATTCAAGAAAGACATGATGCTGTTAAAGAAATAGAGAAAAACTTGATTGAGTTGCATCAAGTATTTTTGGACATGGCAGCACTTGTTGAGTCACAAGGACAACAATTGAATAACATAGAGAGTCATGTTGCACATGCTAGTTCTTTTGTTAGAAGAGGTACTGAACAACTTCATGAGGCAAGAGAGCATCAAAAGGATTCAAGGAAATGGACTTGTTATGTCATACTTCTTGCTATCGTCCTCGTCATCGTGCTCCTCTTTCCATTGTTGATGTCAATTCTACCTCACTTGTTTCTGTAG

Protein sequence

>MS.gene01347.t1

MNDLFSSSFKKYSDLKEQAQIDDVEAGKESINLDKFFEDVENVKEDMRLVEKLYRKLQEANEESKIVHNAKTMKDLRSRMDKDVEQVLKRVKIIKGKLEALERSNAANRNIPGCGPGSSADRTRTSVVSGLGKKLKDMMDDFQGLRARMQQEYKETIERRYFTITGEKADEDTIENLISSGESETFMQRAIQEQGRGQIMDTISEIQERHDAVKEIEKNLIELHQVFLDMAALVESQGQQLNNIESHVAHASSFVRRGTEQLHEAREHQKDSRKWTCYVILLAIVLVIVLLFPLLMSILPHLFL