AlfalfaGEDB | MS.gene01343

M. sativa cultivar XinJiangDaYe / MS.gene01343

KEGG: K08486
GO: GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030674 GO:0033036 GO:0042886 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0060090 GO:0071702 GO:0071705 GO:0110165
PFAM: PF00804.28 PF05739.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01343.t1	XP_024632050.1	99.7	304	1	0	1	304	1	304	2.10E-151	545

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01343.t1	O64791	80.9	282	54	0	1	282	1	282	1.2e-123	444.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01343.t1	G7ILD5	99.7	304	1	0	1	304	1	304	1.5e-151	545.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene033077	MS.gene01343	PPI
MS.gene01343	MS.gene063476	PPI
MS.gene21144	MS.gene01343	PPI
MS.gene01343	MS.gene55383	PPI
MS.gene01343	MS.gene015460	PPI
MS.gene01343	MS.gene99692	PPI
MS.gene01343	MS.gene044431	PPI
MS.gene035781	MS.gene01343	PPI
MS.gene01343	MS.gene69311	PPI
MS.gene01343	MS.gene70948	PPI
MS.gene73141	MS.gene01343	PPI
MS.gene01343	MS.gene09947	PPI
MS.gene01343	MS.gene025786	PPI
MS.gene01343	MS.gene53170	PPI
MS.gene01343	MS.gene064565	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01343.t1	MTR_2g012270	99.671	304	1	0	1	304	1	304	0.0	622
MS.gene01343.t1	MTR_4g090900	90.000	280	27	1	1	279	1	280	0.0	525
MS.gene01343.t1	MTR_1g056550	58.042	286	110	2	1	277	1	285	6.13e-122	352
MS.gene01343.t1	MTR_1g056560	56.701	291	114	1	1	279	1	291	4.61e-119	345
MS.gene01343.t1	MTR_7g106420	57.490	247	105	0	33	279	28	274	3.20e-105	309
MS.gene01343.t1	MTR_5g012010	49.324	296	131	4	1	277	1	296	7.76e-99	293
MS.gene01343.t1	MTR_2g088700	44.444	306	161	3	1	300	1	303	2.52e-87	264
MS.gene01343.t1	MTR_6g028140	46.953	279	145	2	1	277	1	278	2.24e-86	261
MS.gene01343.t1	MTR_2g088700	45.936	283	147	1	1	277	1	283	6.28e-86	260
MS.gene01343.t1	MTR_5g020670	43.206	287	145	4	1	278	1	278	3.75e-70	219
MS.gene01343.t1	MTR_8g073900	39.286	168	72	3	1	160	1	146	2.35e-23	94.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01343.t1	AT1G61290	77.961	304	63	1	1	304	1	300	6.06e-180	498
MS.gene01343.t1	AT1G61290	77.961	304	63	1	1	304	1	300	6.06e-180	498
MS.gene01343.t1	AT1G11250	79.211	279	53	1	1	279	1	274	4.53e-166	463
MS.gene01343.t1	AT4G03330	68.543	302	92	1	1	299	1	302	1.16e-146	414
MS.gene01343.t1	AT3G11820	65.992	247	84	0	31	277	11	257	9.78e-120	347
MS.gene01343.t1	AT3G11820	63.566	258	94	0	20	277	31	288	2.19e-119	347
MS.gene01343.t1	AT3G52400	54.671	289	121	2	1	279	1	289	3.56e-105	310
MS.gene01343.t1	AT3G03800	47.872	282	139	2	1	277	1	279	2.49e-88	266
MS.gene01343.t1	AT3G03800	47.872	282	139	2	1	277	1	279	2.49e-88	266
MS.gene01343.t1	AT1G08560	46.048	291	138	4	1	277	1	286	3.17e-85	258
MS.gene01343.t1	AT5G08080	49.643	280	136	2	1	277	1	278	1.14e-80	246
MS.gene01343.t1	AT5G08080	49.643	280	136	2	1	277	1	278	1.14e-80	246
MS.gene01343.t1	AT5G08080	46.735	291	139	4	1	277	1	289	1.75e-77	239
MS.gene01343.t1	AT2G18260	36.656	311	176	5	1	297	1	304	2.31e-64	205
MS.gene01343.t1	AT2G18260	36.656	311	176	5	1	297	1	304	2.31e-64	205
MS.gene01343.t1	AT5G08080	47.030	202	102	2	1	199	1	200	7.09e-45	152
MS.gene01343.t1	AT5G08080	47.030	202	102	2	1	199	1	200	7.09e-45	152

Find 39 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGCTTCTACATCATCAATT+TGG	0.171423	2.2:+70064437	None:intergenic
CAAAAGAGTCAAAATCATTA+AGG	0.241294	2.2:-70064230	MS.gene01343:CDS
ACAAGGTAGGGGTCAGATTA+TGG	0.287097	2.2:-70063915	MS.gene01343:CDS
TGCTTCTACATCATCAATTT+GGG	0.291794	2.2:+70064438	None:intergenic
CCCTGGAATGTTTCTATTTG+CGG	0.343881	2.2:+70064177	None:intergenic
CAACCTTGATAAATTCTTTG+AGG	0.373925	2.2:-70064401	MS.gene01343:CDS
ACTTCAGTGGTTAGTGGATT+GGG	0.377345	2.2:-70064121	MS.gene01343:CDS
AACTTCAGTGGTTAGTGGAT+TGG	0.416019	2.2:-70064122	MS.gene01343:CDS
ATCAAAAGGATTCAAGGAAA+TGG	0.420191	2.2:-70063691	MS.gene01343:CDS
GATGGATAAAGATGTTGAAC+AGG	0.436094	2.2:-70064257	MS.gene01343:CDS
AAAAGAGTCAAAATCATTAA+GGG	0.451614	2.2:-70064229	MS.gene01343:CDS
AACATTCCAGGGTGTGGTCC+AGG	0.464073	2.2:-70064166	MS.gene01343:CDS
GTTCTATCTGCTGATGAACC+TGG	0.467339	2.2:+70064148	None:intergenic
GCCGCAAATAGAAACATTCC+AGG	0.468166	2.2:-70064178	MS.gene01343:CDS
GAGAGCATCAAAAGGATTCA+AGG	0.474516	2.2:-70063697	MS.gene01343:CDS
ACAAGAACTTCAGTGGTTAG+TGG	0.481738	2.2:-70064127	MS.gene01343:CDS
ATTGAGAATTTGATATCAAG+TGG	0.510431	2.2:-70063974	MS.gene01343:CDS
CATCCTCAAAGAATTTATCA+AGG	0.518396	2.2:+70064398	None:intergenic
CAAATTGATGATGTAGAAGC+AGG	0.522039	2.2:-70064436	MS.gene01343:CDS
AGCAATTCAAGAACAAGGTA+GGG	0.529179	2.2:-70063927	MS.gene01343:CDS
GAGCAATTCAAGAACAAGGT+AGG	0.536020	2.2:-70063928	MS.gene01343:CDS
CAATGAAAGATCTTAGATCA+AGG	0.542118	2.2:-70064279	MS.gene01343:CDS
GAAAGATCTTAGATCAAGGA+TGG	0.545537	2.2:-70064275	MS.gene01343:CDS
ACAAAGAAACAATTGAAAGA+AGG	0.546398	2.2:-70064036	MS.gene01343:CDS
GATGAACCTGGACCACACCC+TGG	0.554068	2.2:+70064160	None:intergenic
TGAGGCAAGAGAGCATCAAA+AGG	0.557741	2.2:-70063705	MS.gene01343:CDS
TTGACATCAACAATGGAAAG+AGG	0.573552	2.2:+70063624	None:intergenic
AATAGAAACATTCCAGGGTG+TGG	0.577755	2.2:-70064172	MS.gene01343:CDS
CAAAGAGCAATTCAAGAACA+AGG	0.592503	2.2:-70063932	MS.gene01343:CDS
GAAAGAGGAGCACGATGACG+AGG	0.604139	2.2:+70063639	None:intergenic
GCTCTATAGAAAATTACAAG+AGG	0.608759	2.2:-70064338	MS.gene01343:CDS
GGTAGAATTGACATCAACAA+TGG	0.610196	2.2:+70063617	None:intergenic
GCAATTCAAGAACAAGGTAG+GGG	0.620511	2.2:-70063926	MS.gene01343:CDS
AGAATGTGAAAGAAGACATG+AGG	0.630310	2.2:-70064372	MS.gene01343:CDS
AGATAGAACAAGAACTTCAG+TGG	0.645922	2.2:-70064134	MS.gene01343:CDS
AGGTACTGAACAACTTCATG+AGG	0.661031	2.2:-70063723	MS.gene01343:CDS
GCAGCACTTGTTGAGTCACA+AGG	0.663060	2.2:-70063803	MS.gene01343:CDS
CCGCAAATAGAAACATTCCA+GGG	0.686589	2.2:-70064177	MS.gene01343:CDS
TAATTCTACAGAAACAAGTG+AGG	0.702509	2.2:+70063596	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAACTCAAAGATATGA+TGG	-	chr2.2:70063996-70064015	MS.gene01343:CDS	20.0%
!!	AAAAGAGTCAAAATCATTAA+GGG	-	chr2.2:70063865-70063884	MS.gene01343:CDS	20.0%
!	ACAAAGAAACAATTGAAAGA+AGG	-	chr2.2:70064058-70064077	MS.gene01343:CDS	25.0%
!	ATTGAGAATTTGATATCAAG+TGG	-	chr2.2:70064120-70064139	MS.gene01343:CDS	25.0%
!	CAAAAGAGTCAAAATCATTA+AGG	-	chr2.2:70063864-70063883	MS.gene01343:CDS	25.0%
!!!	AGAAGGTATTTTACAATAAC+TGG	-	chr2.2:70064075-70064094	MS.gene01343:CDS	25.0%
!!!	ATATGATGGATGATTTTCAA+GGG	-	chr2.2:70064010-70064029	MS.gene01343:CDS	25.0%
	ATCAAAAGGATTCAAGGAAA+TGG	-	chr2.2:70064403-70064422	MS.gene01343:CDS	30.0%
	CAAAAAATACAGTGACTTGA+AGG	-	chr2.2:70063627-70063646	MS.gene01343:CDS	30.0%
	CAACCTTGATAAATTCTTTG+AGG	-	chr2.2:70063693-70063712	MS.gene01343:CDS	30.0%
	CAATGAAAGATCTTAGATCA+AGG	-	chr2.2:70063815-70063834	MS.gene01343:CDS	30.0%
	CATCCTCAAAGAATTTATCA+AGG	+	chr2.2:70063699-70063718	None:intergenic	30.0%
	GCTCTATAGAAAATTACAAG+AGG	-	chr2.2:70063756-70063775	MS.gene01343:CDS	30.0%
	TGCTTCTACATCATCAATTT+GGG	+	chr2.2:70063659-70063678	None:intergenic	30.0%
!	TGAGTTGCATCAAGTATTTT+TGG	-	chr2.2:70064263-70064282	MS.gene01343:CDS	30.0%
!!!	CAAGTCACTGTATTTTTTGA+AGG	+	chr2.2:70063626-70063645	None:intergenic	30.0%
!!!	CTTTCATTGTTTTAGCATTG+TGG	+	chr2.2:70063804-70063823	None:intergenic	30.0%
!!!	GATATGATGGATGATTTTCA+AGG	-	chr2.2:70064009-70064028	MS.gene01343:CDS	30.0%
	AGAATGTGAAAGAAGACATG+AGG	-	chr2.2:70063722-70063741	MS.gene01343:CDS	35.0%
	AGATAGAACAAGAACTTCAG+TGG	-	chr2.2:70063960-70063979	MS.gene01343:CDS	35.0%
	AGCAATTCAAGAACAAGGTA+GGG	-	chr2.2:70064167-70064186	MS.gene01343:CDS	35.0%
	CAAAGAGCAATTCAAGAACA+AGG	-	chr2.2:70064162-70064181	MS.gene01343:CDS	35.0%
	CTGCTTCTACATCATCAATT+TGG	+	chr2.2:70063660-70063679	None:intergenic	35.0%
	GAAAGATCTTAGATCAAGGA+TGG	-	chr2.2:70063819-70063838	MS.gene01343:CDS	35.0%
	GATGGATAAAGATGTTGAAC+AGG	-	chr2.2:70063837-70063856	MS.gene01343:CDS	35.0%
	GGTAGAATTGACATCAACAA+TGG	+	chr2.2:70064480-70064499	None:intergenic	35.0%
	TTGACATCAACAATGGAAAG+AGG	+	chr2.2:70064473-70064492	None:intergenic	35.0%
!!	CAAATTGATGATGTAGAAGC+AGG	-	chr2.2:70063658-70063677	MS.gene01343:CDS	35.0%
!!	GCTAGTTCTTTTGTTAGAAG+AGG	-	chr2.2:70064351-70064370	MS.gene01343:CDS	35.0%
!!!	GCATCAAGTATTTTTGGACA+TGG	-	chr2.2:70064269-70064288	MS.gene01343:CDS	35.0%
	AATAGAAACATTCCAGGGTG+TGG	-	chr2.2:70063922-70063941	MS.gene01343:CDS	40.0%
	ACAAGAACTTCAGTGGTTAG+TGG	-	chr2.2:70063967-70063986	MS.gene01343:CDS	40.0%
	CCGCAAATAGAAACATTCCA+GGG	-	chr2.2:70063917-70063936	MS.gene01343:CDS	40.0%
	GAGCAATTCAAGAACAAGGT+AGG	-	chr2.2:70064166-70064185	MS.gene01343:CDS	40.0%
	GCAATTCAAGAACAAGGTAG+GGG	-	chr2.2:70064168-70064187	MS.gene01343:CDS	40.0%
!	AACTTCAGTGGTTAGTGGAT+TGG	-	chr2.2:70063972-70063991	MS.gene01343:CDS	40.0%
!	CCCTGGAATGTTTCTATTTG+CGG	+	chr2.2:70063920-70063939	None:intergenic	40.0%
!!	ACTTCAGTGGTTAGTGGATT+GGG	-	chr2.2:70063973-70063992	MS.gene01343:CDS	40.0%
!!	AGGTACTGAACAACTTCATG+AGG	-	chr2.2:70064371-70064390	MS.gene01343:CDS	40.0%
!!	GAGAGCATCAAAAGGATTCA+AGG	-	chr2.2:70064397-70064416	MS.gene01343:CDS	40.0%
	ACAAGGTAGGGGTCAGATTA+TGG	-	chr2.2:70064179-70064198	MS.gene01343:CDS	45.0%
	GCCGCAAATAGAAACATTCC+AGG	-	chr2.2:70063916-70063935	MS.gene01343:CDS	45.0%
	GTTCTATCTGCTGATGAACC+TGG	+	chr2.2:70063949-70063968	None:intergenic	45.0%
!!	TGAGGCAAGAGAGCATCAAA+AGG	-	chr2.2:70064389-70064408	MS.gene01343:CDS	45.0%
!	GCAGCACTTGTTGAGTCACA+AGG	-	chr2.2:70064291-70064310	MS.gene01343:CDS	50.0%
	AACATTCCAGGGTGTGGTCC+AGG	-	chr2.2:70063928-70063947	MS.gene01343:CDS	55.0%
!	GAAAGAGGAGCACGATGACG+AGG	+	chr2.2:70064458-70064477	None:intergenic	55.0%
	GATGAACCTGGACCACACCC+TGG	+	chr2.2:70063937-70063956	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	70063601	70064515	70063601	ID=MS.gene01343
chr2.2	mRNA	70063601	70064515	70063601	ID=MS.gene01343.t1;Parent=MS.gene01343
chr2.2	exon	70063601	70064515	70063601	ID=MS.gene01343.t1.exon1;Parent=MS.gene01343.t1
chr2.2	CDS	70063601	70064515	70063601	ID=cds.MS.gene01343.t1;Parent=MS.gene01343.t1

Gene Sequence

>MS.gene01343

ATGAATGACTTATTCTCAAGCTCCTTCAAAAAATACAGTGACTTGAAGGAGCAAGCCCAAATTGATGATGTAGAAGCAGGAAAAGAGAGTATCAACCTTGATAAATTCTTTGAGGATGTTGAGAATGTGAAAGAAGACATGAGGCTTGTTGAGAAGCTCTATAGAAAATTACAAGAGGCAAATGAAGAAAGCAAAATTGTCCACAATGCTAAAACAATGAAAGATCTTAGATCAAGGATGGATAAAGATGTTGAACAGGTTCTCAAAAGAGTCAAAATCATTAAGGGTAAGCTTGAAGCATTAGAACGTTCGAATGCCGCAAATAGAAACATTCCAGGGTGTGGTCCAGGTTCATCAGCAGATAGAACAAGAACTTCAGTGGTTAGTGGATTGGGAAAAAAACTCAAAGATATGATGGATGATTTTCAAGGGTTAAGAGCAAGAATGCAACAAGAGTACAAAGAAACAATTGAAAGAAGGTATTTTACAATAACTGGTGAGAAAGCTGATGAAGATACTATTGAGAATTTGATATCAAGTGGAGAAAGTGAAACTTTTATGCAAAGAGCAATTCAAGAACAAGGTAGGGGTCAGATTATGGACACAATATCTGAGATTCAAGAAAGACATGATGCTGTTAAAGAAATAGAGAAAAACTTGATTGAGTTGCATCAAGTATTTTTGGACATGGCAGCACTTGTTGAGTCACAAGGACAACAATTGAATAACATAGAGAGTCATGTTGCACATGCTAGTTCTTTTGTTAGAAGAGGTACTGAACAACTTCATGAGGCAAGAGAGCATCAAAAGGATTCAAGGAAATGGACTTGTTATGTCATACTTCTTGCTATCGTCCTCGTCATCGTGCTCCTCTTTCCATTGTTGATGTCAATTCTACCTCACTTGTTTCTGTAG

Protein sequence

>MS.gene01343.t1

MNDLFSSSFKKYSDLKEQAQIDDVEAGKESINLDKFFEDVENVKEDMRLVEKLYRKLQEANEESKIVHNAKTMKDLRSRMDKDVEQVLKRVKIIKGKLEALERSNAANRNIPGCGPGSSADRTRTSVVSGLGKKLKDMMDDFQGLRARMQQEYKETIERRYFTITGEKADEDTIENLISSGESETFMQRAIQEQGRGQIMDTISEIQERHDAVKEIEKNLIELHQVFLDMAALVESQGQQLNNIESHVAHASSFVRRGTEQLHEAREHQKDSRKWTCYVILLAIVLVIVLLFPLLMSILPHLFL