Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025786.t1 | XP_003611259.1 | 97.7 | 301 | 7 | 0 | 1 | 301 | 20 | 320 | 4.10E-147 | 530.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025786.t1 | Q42374 | 65.7 | 297 | 93 | 3 | 6 | 301 | 22 | 310 | 6.8e-90 | 332.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025786.t1 | G7KG89 | 97.7 | 301 | 7 | 0 | 1 | 301 | 20 | 320 | 3.0e-147 | 530.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene025786 | MS.gene035217 | PPI |
| MS.gene77217 | MS.gene025786 | PPI |
| MS.gene01756 | MS.gene025786 | PPI |
| MS.gene65117 | MS.gene025786 | PPI |
| MS.gene025786 | MS.gene01347 | PPI |
| MS.gene66057 | MS.gene025786 | PPI |
| MS.gene025786 | MS.gene052542 | PPI |
| MS.gene047508 | MS.gene025786 | PPI |
| MS.gene025786 | MS.gene78212 | PPI |
| MS.gene066610 | MS.gene025786 | PPI |
| MS.gene33736 | MS.gene025786 | PPI |
| MS.gene99119 | MS.gene025786 | PPI |
| MS.gene01343 | MS.gene025786 | PPI |
| MS.gene32192 | MS.gene025786 | PPI |
| MS.gene025786 | MS.gene50283 | PPI |
| MS.gene002374 | MS.gene025786 | PPI |
| MS.gene99067 | MS.gene025786 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025786.t1 | MTR_5g012010 | 98.007 | 301 | 6 | 0 | 1 | 301 | 20 | 320 | 0.0 | 598 |
| MS.gene025786.t1 | MTR_2g012270 | 48.951 | 286 | 140 | 2 | 12 | 297 | 18 | 297 | 6.69e-100 | 295 |
| MS.gene025786.t1 | MTR_4g090900 | 53.036 | 247 | 115 | 1 | 31 | 277 | 33 | 278 | 2.26e-93 | 279 |
| MS.gene025786.t1 | MTR_1g056550 | 45.769 | 260 | 140 | 1 | 18 | 277 | 27 | 285 | 3.75e-84 | 255 |
| MS.gene025786.t1 | MTR_1g056560 | 48.583 | 247 | 126 | 1 | 31 | 277 | 44 | 289 | 1.32e-83 | 255 |
| MS.gene025786.t1 | MTR_2g088700 | 44.710 | 293 | 151 | 2 | 6 | 298 | 23 | 304 | 7.25e-78 | 239 |
| MS.gene025786.t1 | MTR_7g106420 | 45.714 | 245 | 132 | 1 | 35 | 279 | 31 | 274 | 4.10e-77 | 237 |
| MS.gene025786.t1 | MTR_6g028140 | 43.396 | 265 | 149 | 1 | 15 | 279 | 17 | 280 | 7.54e-74 | 229 |
| MS.gene025786.t1 | MTR_2g088700 | 45.588 | 272 | 137 | 2 | 6 | 277 | 23 | 283 | 1.69e-73 | 228 |
| MS.gene025786.t1 | MTR_5g020670 | 45.091 | 275 | 137 | 5 | 5 | 276 | 13 | 276 | 3.64e-68 | 214 |
| MS.gene025786.t1 | MTR_8g073900 | 33.544 | 158 | 79 | 3 | 5 | 160 | 13 | 146 | 3.15e-15 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025786.t1 | AT1G08560 | 63.973 | 297 | 98 | 4 | 6 | 301 | 22 | 310 | 1.72e-120 | 348 |
| MS.gene025786.t1 | AT1G61290 | 51.515 | 264 | 125 | 2 | 32 | 293 | 31 | 293 | 3.98e-97 | 288 |
| MS.gene025786.t1 | AT1G61290 | 51.515 | 264 | 125 | 2 | 32 | 293 | 31 | 293 | 3.98e-97 | 288 |
| MS.gene025786.t1 | AT1G11250 | 50.000 | 266 | 127 | 2 | 12 | 277 | 13 | 272 | 5.08e-91 | 273 |
| MS.gene025786.t1 | AT4G03330 | 51.685 | 267 | 128 | 1 | 31 | 297 | 35 | 300 | 9.73e-91 | 272 |
| MS.gene025786.t1 | AT3G11820 | 52.632 | 247 | 116 | 1 | 31 | 277 | 12 | 257 | 1.11e-87 | 265 |
| MS.gene025786.t1 | AT3G11820 | 50.190 | 263 | 130 | 1 | 15 | 277 | 27 | 288 | 1.39e-87 | 266 |
| MS.gene025786.t1 | AT3G52400 | 45.318 | 267 | 141 | 2 | 17 | 279 | 24 | 289 | 5.55e-78 | 241 |
| MS.gene025786.t1 | AT3G03800 | 41.216 | 296 | 161 | 4 | 16 | 299 | 7 | 301 | 3.68e-69 | 217 |
| MS.gene025786.t1 | AT3G03800 | 41.216 | 296 | 161 | 4 | 16 | 299 | 7 | 301 | 3.68e-69 | 217 |
| MS.gene025786.t1 | AT5G08080 | 50.685 | 219 | 107 | 1 | 59 | 277 | 61 | 278 | 1.35e-65 | 208 |
| MS.gene025786.t1 | AT5G08080 | 50.685 | 219 | 107 | 1 | 59 | 277 | 61 | 278 | 1.35e-65 | 208 |
| MS.gene025786.t1 | AT5G08080 | 50.685 | 219 | 107 | 1 | 59 | 277 | 72 | 289 | 1.45e-65 | 208 |
| MS.gene025786.t1 | AT2G18260 | 38.662 | 269 | 157 | 3 | 29 | 297 | 44 | 304 | 1.77e-62 | 200 |
| MS.gene025786.t1 | AT2G18260 | 38.662 | 269 | 157 | 3 | 29 | 297 | 44 | 304 | 1.77e-62 | 200 |
| MS.gene025786.t1 | AT5G08080 | 47.518 | 141 | 73 | 1 | 59 | 199 | 61 | 200 | 1.48e-30 | 115 |
| MS.gene025786.t1 | AT5G08080 | 47.518 | 141 | 73 | 1 | 59 | 199 | 61 | 200 | 1.48e-30 | 115 |
Find 46 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGATTGAGAAGAGTTTAT+TGG | 0.159342 | 5.3:-5317923 | MS.gene025786:CDS |
| GGAGATGATGAAAGTTTCTT+AGG | 0.183110 | 5.3:-5318024 | MS.gene025786:CDS |
| GGAGCTTATGATGGAGTTTC+AGG | 0.211805 | 5.3:-5318406 | MS.gene025786:intron |
| TGAAGAAAGCAAAGTGTATT+AGG | 0.275539 | 5.3:-5318554 | MS.gene025786:CDS |
| AACTGATATGAATTTGTTCT+TGG | 0.340603 | 5.3:-5318727 | MS.gene025786:CDS |
| ACTAGAATTGCTGTTACTAA+TGG | 0.388743 | 5.3:-5318447 | MS.gene025786:CDS |
| ACATGGAAGAGGAAAGGTTC+TGG | 0.392183 | 5.3:-5317986 | MS.gene025786:CDS |
| AAGATCTTCACTGACCCATT+TGG | 0.394651 | 5.3:-5318751 | MS.gene025786:CDS |
| AATTCATATCAGTTTCCAAA+TGG | 0.400693 | 5.3:+5318736 | None:intergenic |
| TGAGAAAGTAAGAACAGAAA+TGG | 0.403034 | 5.3:-5318697 | MS.gene025786:CDS |
| ATTCTAGTTCTATAAATTGC+TGG | 0.404484 | 5.3:+5318462 | None:intergenic |
| TAGGACTCAACTCGAAGAAA+TGG | 0.407461 | 5.3:-5318535 | MS.gene025786:CDS |
| TGAAGCACAAGGTGAGAAAA+TGG | 0.413624 | 5.3:-5317863 | MS.gene025786:CDS |
| GATGAAGTTGATTTAGAAGC+TGG | 0.444831 | 5.3:-5318807 | MS.gene025786:CDS |
| GCAGGAAGTGGATGTGCATT+GGG | 0.446829 | 5.3:-5317750 | MS.gene025786:CDS |
| AGAAGATATTATACAGTGAC+AGG | 0.449572 | 5.3:-5318090 | MS.gene025786:CDS |
| TGAAGCTTTGAAAGCATTGA+GGG | 0.449699 | 5.3:-5318607 | MS.gene025786:CDS |
| CTGCTGAAACTCACTACAAT+GGG | 0.453019 | 5.3:+5317688 | None:intergenic |
| GCTGCTAATAGAAGACTCTC+TGG | 0.458709 | 5.3:-5318501 | MS.gene025786:CDS |
| ATTCATATCAGTTTCCAAAT+GGG | 0.460811 | 5.3:+5318737 | None:intergenic |
| GCTTCTCATTATGTTAAAGA+TGG | 0.464968 | 5.3:-5317814 | MS.gene025786:CDS |
| GAGATGATGAAAGTTTCTTA+GGG | 0.469669 | 5.3:-5318023 | MS.gene025786:CDS |
| TATGAATTTGTTCTTGGAAG+AGG | 0.470619 | 5.3:-5318721 | MS.gene025786:CDS |
| AGCAGGAAGTGGATGTGCAT+TGG | 0.476019 | 5.3:-5317751 | MS.gene025786:CDS |
| ATTGAGAAGATAATATCAAA+TGG | 0.477269 | 5.3:-5318045 | MS.gene025786:CDS |
| ATCAGAAAAGTAGCAGGAAG+TGG | 0.480407 | 5.3:-5317762 | MS.gene025786:CDS |
| AACAGTATCAGAAAAGTAGC+AGG | 0.504319 | 5.3:-5317768 | MS.gene025786:CDS |
| CTGAAGCTTTGAAAGCATTG+AGG | 0.521475 | 5.3:-5318608 | MS.gene025786:CDS |
| GAAACTCAAGGAGCTTATGA+TGG | 0.521896 | 5.3:-5318415 | MS.gene025786:CDS |
| GATGGTACAAAGAATCTTCA+CGG | 0.525784 | 5.3:-5317796 | MS.gene025786:CDS |
| GTATGTTACAACAGGGGCTG+AGG | 0.531696 | 5.3:-5318149 | MS.gene025786:intron |
| GAGAAAGTAAGAACAGAAAT+GGG | 0.536281 | 5.3:-5318696 | MS.gene025786:CDS |
| AGTGAATACAAAGAAACAGT+AGG | 0.536819 | 5.3:-5318114 | MS.gene025786:CDS |
| TGATGAAAGTTTCTTAGGGA+AGG | 0.540453 | 5.3:-5318019 | MS.gene025786:CDS |
| AGACTCTCTGGACTTAAAGA+TGG | 0.542560 | 5.3:-5318489 | MS.gene025786:CDS |
| GCAATACAAGAACATGGAAG+AGG | 0.566682 | 5.3:-5317997 | MS.gene025786:CDS |
| ACTGCTGAAACTCACTACAA+TGG | 0.567938 | 5.3:+5317687 | None:intergenic |
| GTGAATACAAAGAAACAGTA+GGG | 0.579417 | 5.3:-5318113 | MS.gene025786:CDS |
| TGGCTTGCGTAAGAAACTCA+AGG | 0.596215 | 5.3:-5318427 | MS.gene025786:CDS |
| AGGGGAACATGCTGATGAAG+AGG | 0.599762 | 5.3:-5318070 | MS.gene025786:CDS |
| ACAAGAACATGGAAGAGGAA+AGG | 0.606969 | 5.3:-5317992 | MS.gene025786:CDS |
| GGGAAGGCAATACAAGAACA+TGG | 0.609551 | 5.3:-5318003 | MS.gene025786:CDS |
| AGGAAAGGTTCTGGAAACTG+TGG | 0.660613 | 5.3:-5317977 | MS.gene025786:CDS |
| GAAGATATTATACAGTGACA+GGG | 0.694420 | 5.3:-5318089 | MS.gene025786:CDS |
| GCTGTTATGGTTGAAGCACA+AGG | 0.705464 | 5.3:-5317874 | MS.gene025786:CDS |
| AAGATATTATACAGTGACAG+GGG | 0.720898 | 5.3:-5318088 | MS.gene025786:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTAGTCTTATTTAATA+AGG | + | chr5.3:5318242-5318261 | None:intergenic | 15.0% |
| !!! | GAATTTATGTATCAATTTTA+AGG | - | chr5.3:5318180-5318199 | MS.gene025786:intron | 15.0% |
| !! | ATTGAGAAGATAATATCAAA+TGG | - | chr5.3:5318449-5318468 | MS.gene025786:CDS | 20.0% |
| !!! | TCATCAAATATTTTTGGATA+TGG | - | chr5.3:5318598-5318617 | MS.gene025786:CDS | 20.0% |
| ! | AATTCATATCAGTTTCCAAA+TGG | + | chr5.3:5317761-5317780 | None:intergenic | 25.0% |
| ! | ATTCATATCAGTTTCCAAAT+GGG | + | chr5.3:5317760-5317779 | None:intergenic | 25.0% |
| ! | TCAGATAATGTCATATTCTA+AGG | - | chr5.3:5318260-5318279 | MS.gene025786:intron | 25.0% |
| !! | AACTGATATGAATTTGTTCT+TGG | - | chr5.3:5317767-5317786 | MS.gene025786:CDS | 25.0% |
| !! | GATTATACTTTTGCTTATTC+TGG | - | chr5.3:5318766-5318785 | MS.gene025786:CDS | 25.0% |
| !! | GGAACTTCATCAAATATTTT+TGG | - | chr5.3:5318592-5318611 | MS.gene025786:CDS | 25.0% |
| !!! | ATTCTAGTTCTATAAATTGC+TGG | + | chr5.3:5318035-5318054 | None:intergenic | 25.0% |
| AAACCTTGTATGTTACAACA+GGG | - | chr5.3:5318338-5318357 | MS.gene025786:intron | 30.0% | |
| AAGATATTATACAGTGACAG+GGG | - | chr5.3:5318406-5318425 | MS.gene025786:intron | 30.0% | |
| AGAAGATATTATACAGTGAC+AGG | - | chr5.3:5318404-5318423 | MS.gene025786:intron | 30.0% | |
| AGAGATTGAGAAGAGTTTAT+TGG | - | chr5.3:5318571-5318590 | MS.gene025786:CDS | 30.0% | |
| AGTGAATACAAAGAAACAGT+AGG | - | chr5.3:5318380-5318399 | MS.gene025786:intron | 30.0% | |
| GAAGATATTATACAGTGACA+GGG | - | chr5.3:5318405-5318424 | MS.gene025786:intron | 30.0% | |
| GAGAAAGTAAGAACAGAAAT+GGG | - | chr5.3:5317798-5317817 | MS.gene025786:CDS | 30.0% | |
| GAGATGATGAAAGTTTCTTA+GGG | - | chr5.3:5318471-5318490 | MS.gene025786:CDS | 30.0% | |
| GCTTCTCATTATGTTAAAGA+TGG | - | chr5.3:5318680-5318699 | MS.gene025786:CDS | 30.0% | |
| GTGAATACAAAGAAACAGTA+GGG | - | chr5.3:5318381-5318400 | MS.gene025786:intron | 30.0% | |
| TGAAGAAAGCAAAGTGTATT+AGG | - | chr5.3:5317940-5317959 | MS.gene025786:CDS | 30.0% | |
| TGAGAAAGTAAGAACAGAAA+TGG | - | chr5.3:5317797-5317816 | MS.gene025786:CDS | 30.0% | |
| ! | ACTAGAATTGCTGTTACTAA+TGG | - | chr5.3:5318047-5318066 | MS.gene025786:CDS | 30.0% |
| ! | TATGAATTTGTTCTTGGAAG+AGG | - | chr5.3:5317773-5317792 | MS.gene025786:CDS | 30.0% |
| !! | ATTTTTGGATATGGCTGTTA+TGG | - | chr5.3:5318607-5318626 | MS.gene025786:CDS | 30.0% |
| !!! | TCTTCTGGTTTTTTTCATTC+TGG | - | chr5.3:5318131-5318150 | MS.gene025786:CDS | 30.0% |
| AACAGTATCAGAAAAGTAGC+AGG | - | chr5.3:5318726-5318745 | MS.gene025786:CDS | 35.0% | |
| AACCAGAAGAGAAATTGACA+AGG | + | chr5.3:5318121-5318140 | None:intergenic | 35.0% | |
| AACCTTGTATGTTACAACAG+GGG | - | chr5.3:5318339-5318358 | MS.gene025786:intron | 35.0% | |
| ACCAGAAGAGAAATTGACAA+GGG | + | chr5.3:5318120-5318139 | None:intergenic | 35.0% | |
| GAAACCTTGTATGTTACAAC+AGG | - | chr5.3:5318337-5318356 | MS.gene025786:intron | 35.0% | |
| GATGGTACAAAGAATCTTCA+CGG | - | chr5.3:5318698-5318717 | MS.gene025786:CDS | 35.0% | |
| GGAGATGATGAAAGTTTCTT+AGG | - | chr5.3:5318470-5318489 | MS.gene025786:CDS | 35.0% | |
| TGATGAAAGTTTCTTAGGGA+AGG | - | chr5.3:5318475-5318494 | MS.gene025786:CDS | 35.0% | |
| ! | GATGAAGTTGATTTAGAAGC+TGG | - | chr5.3:5317687-5317706 | MS.gene025786:CDS | 35.0% |
| !! | TGAAGCTTTGAAAGCATTGA+GGG | - | chr5.3:5317887-5317906 | MS.gene025786:CDS | 35.0% |
| AAGATCTTCACTGACCCATT+TGG | - | chr5.3:5317743-5317762 | MS.gene025786:CDS | 40.0% | |
| ACAAGAACATGGAAGAGGAA+AGG | - | chr5.3:5318502-5318521 | MS.gene025786:CDS | 40.0% | |
| ACCCTTGTCAATTTCTCTTC+TGG | - | chr5.3:5318116-5318135 | MS.gene025786:CDS | 40.0% | |
| ACTGCTGAAACTCACTACAA+TGG | + | chr5.3:5318810-5318829 | None:intergenic | 40.0% | |
| AGACTCTCTGGACTTAAAGA+TGG | - | chr5.3:5318005-5318024 | MS.gene025786:CDS | 40.0% | |
| ATCAGAAAAGTAGCAGGAAG+TGG | - | chr5.3:5318732-5318751 | MS.gene025786:CDS | 40.0% | |
| CTGCTGAAACTCACTACAAT+GGG | + | chr5.3:5318809-5318828 | None:intergenic | 40.0% | |
| GAAACTCAAGGAGCTTATGA+TGG | - | chr5.3:5318079-5318098 | MS.gene025786:CDS | 40.0% | |
| GCAATACAAGAACATGGAAG+AGG | - | chr5.3:5318497-5318516 | MS.gene025786:CDS | 40.0% | |
| TAGGACTCAACTCGAAGAAA+TGG | - | chr5.3:5317959-5317978 | MS.gene025786:CDS | 40.0% | |
| TGAAGCACAAGGTGAGAAAA+TGG | - | chr5.3:5318631-5318650 | MS.gene025786:CDS | 40.0% | |
| !! | CTGAAGCTTTGAAAGCATTG+AGG | - | chr5.3:5317886-5317905 | MS.gene025786:CDS | 40.0% |
| !! | GAAGCTGGTCTTCAAAAACA+AGG | - | chr5.3:5317702-5317721 | MS.gene025786:CDS | 40.0% |
| ACATGGAAGAGGAAAGGTTC+TGG | - | chr5.3:5318508-5318527 | MS.gene025786:CDS | 45.0% | |
| AGCCCCTGTTGTAACATACA+AGG | + | chr5.3:5318344-5318363 | None:intergenic | 45.0% | |
| GCTGCTAATAGAAGACTCTC+TGG | - | chr5.3:5317993-5318012 | MS.gene025786:CDS | 45.0% | |
| GCTGTTATGGTTGAAGCACA+AGG | - | chr5.3:5318620-5318639 | MS.gene025786:CDS | 45.0% | |
| GGGAAGGCAATACAAGAACA+TGG | - | chr5.3:5318491-5318510 | MS.gene025786:CDS | 45.0% | |
| ! | GGAGCTTATGATGGAGTTTC+AGG | - | chr5.3:5318088-5318107 | MS.gene025786:CDS | 45.0% |
| ! | TGGCTTGCGTAAGAAACTCA+AGG | - | chr5.3:5318067-5318086 | MS.gene025786:CDS | 45.0% |
| !! | AGGAAAGGTTCTGGAAACTG+TGG | - | chr5.3:5318517-5318536 | MS.gene025786:CDS | 45.0% |
| !! | GCTGGTCTTCAAAAACAAGG+TGG | - | chr5.3:5317705-5317724 | MS.gene025786:CDS | 45.0% |
| AGCAGGAAGTGGATGTGCAT+TGG | - | chr5.3:5318743-5318762 | MS.gene025786:CDS | 50.0% | |
| AGGGGAACATGCTGATGAAG+AGG | - | chr5.3:5318424-5318443 | MS.gene025786:CDS | 50.0% | |
| GCAGGAAGTGGATGTGCATT+GGG | - | chr5.3:5318744-5318763 | MS.gene025786:CDS | 50.0% | |
| GTATGTTACAACAGGGGCTG+AGG | - | chr5.3:5318345-5318364 | MS.gene025786:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 5317681 | 5318835 | 5317681 | ID=MS.gene025786 |
| chr5.3 | mRNA | 5317681 | 5318835 | 5317681 | ID=MS.gene025786.t1;Parent=MS.gene025786 |
| chr5.3 | exon | 5318407 | 5318835 | 5318407 | ID=MS.gene025786.t1.exon1;Parent=MS.gene025786.t1 |
| chr5.3 | CDS | 5318407 | 5318835 | 5318407 | ID=cds.MS.gene025786.t1;Parent=MS.gene025786.t1 |
| chr5.3 | exon | 5317681 | 5318157 | 5317681 | ID=MS.gene025786.t1.exon2;Parent=MS.gene025786.t1 |
| chr5.3 | CDS | 5317681 | 5318157 | 5317681 | ID=cds.MS.gene025786.t1;Parent=MS.gene025786.t1 |
| Gene Sequence |
| Protein sequence |
