AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028134

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028134.t1 MTR_5g026320 96.943 458 14 0 1 458 1 458 0.0 924
MS.gene028134.t1 MTR_3g450710 36.682 428 244 12 53 456 44 468 5.10e-75 243
MS.gene028134.t1 MTR_5g090830 36.219 439 249 14 15 428 7 439 2.81e-74 241
MS.gene028134.t1 MTR_5g090810 35.927 437 253 14 15 428 7 439 1.84e-73 239
MS.gene028134.t1 MTR_3g450690 37.136 412 235 11 67 457 65 473 3.93e-72 235
MS.gene028134.t1 MTR_5g090880 37.688 398 221 12 56 428 46 441 1.84e-71 234
MS.gene028134.t1 MTR_4g007540 25.178 421 280 13 42 444 25 428 2.27e-26 111
MS.gene028134.t1 MTR_8g096530 26.434 401 257 13 41 424 28 407 6.07e-26 110
MS.gene028134.t1 MTR_1g053315 25.293 427 282 16 34 437 35 447 1.43e-25 109
MS.gene028134.t1 MTR_5g076700 24.336 452 282 16 16 437 8 429 3.33e-25 108
MS.gene028134.t1 MTR_2g064495 24.155 414 278 14 42 437 25 420 3.80e-25 107
MS.gene028134.t1 MTR_7g114470 25.000 436 268 17 33 437 24 431 8.97e-25 106
MS.gene028134.t1 MTR_2g105330 24.599 374 257 12 42 401 25 387 1.61e-24 105
MS.gene028134.t1 MTR_3g098980 25.124 402 260 14 41 424 35 413 8.68e-24 103
MS.gene028134.t1 MTR_7g084940 27.056 377 236 13 41 399 37 392 3.30e-23 102
MS.gene028134.t1 MTR_5g084870 25.293 427 273 13 17 426 2 399 3.58e-23 102
MS.gene028134.t1 MTR_4g022290 25.957 470 276 23 17 447 3 439 5.55e-23 101
MS.gene028134.t1 MTR_3g112180 26.250 400 244 13 33 405 25 400 6.04e-23 101
MS.gene028134.t1 MTR_7g027175 25.815 399 260 14 41 423 37 415 9.63e-23 100
MS.gene028134.t1 MTR_4g127650 26.142 394 252 15 33 397 23 406 1.03e-22 100
MS.gene028134.t1 MTR_8g079970 24.324 444 262 17 17 426 2 405 1.23e-22 100
MS.gene028134.t1 MTR_3g049150 25.301 415 277 12 52 443 36 440 1.24e-22 100
MS.gene028134.t1 MTR_4g097090 25.995 377 234 15 53 397 42 405 1.30e-22 100
MS.gene028134.t1 MTR_8g075610 24.332 374 256 13 42 401 16 376 1.80e-21 96.7
MS.gene028134.t1 MTR_7g085990 27.696 408 225 21 56 417 37 420 6.65e-21 95.5
MS.gene028134.t1 MTR_7g086000 24.645 422 273 15 55 443 41 450 1.92e-20 94.0
MS.gene028134.t1 MTR_8g079980 25.850 441 258 16 17 426 2 404 3.36e-20 93.2
MS.gene028134.t1 MTR_7g114550 25.558 403 253 16 33 414 23 399 4.62e-20 92.8
MS.gene028134.t1 MTR_3g101900 25.658 456 295 18 18 448 7 443 1.91e-19 90.9
MS.gene028134.t1 MTR_8g079950 23.744 438 270 17 17 424 2 405 2.01e-19 90.9
MS.gene028134.t1 MTR_7g114520 24.873 394 253 14 33 404 24 396 2.17e-19 90.9
MS.gene028134.t1 MTR_7g114560 24.129 402 260 16 33 414 23 399 2.68e-19 90.5
MS.gene028134.t1 MTR_7g114490 24.623 398 263 14 33 414 23 399 1.45e-18 88.6
MS.gene028134.t1 MTR_8g467590 26.842 380 217 22 51 400 38 386 2.16e-18 87.8
MS.gene028134.t1 MTR_3g101960 25.942 451 282 17 32 448 13 445 2.91e-18 87.4
MS.gene028134.t1 MTR_4g094842 23.871 465 295 17 18 448 4 443 2.99e-18 87.0
MS.gene028134.t1 MTR_1g040175 26.263 396 238 18 33 402 22 389 2.81e-17 84.3
MS.gene028134.t1 MTR_3g086020 23.923 418 233 19 15 400 2 366 2.90e-17 84.0
MS.gene028134.t1 MTR_7g056087 24.432 352 238 12 33 361 17 363 4.29e-17 83.6
MS.gene028134.t1 MTR_0190s0020 24.729 461 300 19 18 453 4 442 5.52e-17 83.2
MS.gene028134.t1 MTR_3g465990 22.642 318 229 8 56 358 47 362 2.17e-16 81.6
MS.gene028134.t1 MTR_8g467580 25.789 380 229 15 47 400 34 386 3.67e-16 80.9
MS.gene028134.t1 MTR_5g084850 23.131 428 280 13 17 426 2 398 1.09e-15 79.3
MS.gene028134.t1 MTR_4g094835 23.487 413 249 17 71 448 61 441 1.45e-15 79.0
MS.gene028134.t1 MTR_3g465980 23.270 318 227 9 56 358 47 362 1.49e-15 79.0
MS.gene028134.t1 MTR_3g026950 21.462 424 296 13 35 430 17 431 2.67e-15 78.2
MS.gene028134.t1 MTR_1g081660 24.427 393 253 17 41 414 39 406 4.09e-15 77.8
MS.gene028134.t1 MTR_7g084910 25.397 378 227 14 41 399 38 379 5.05e-15 77.4
MS.gene028134.t1 MTR_7g114960 22.430 321 221 11 56 361 45 352 6.34e-15 77.0
MS.gene028134.t1 MTR_3g071320 23.940 401 270 14 21 401 7 392 7.75e-15 76.6
MS.gene028134.t1 MTR_3g465930 24.363 353 236 12 12 340 2 347 8.10e-15 76.6
MS.gene028134.t1 MTR_1g040315 25.000 276 174 9 16 272 7 268 8.76e-15 76.3
MS.gene028134.t1 MTR_3g465910 23.398 359 229 12 12 340 2 344 5.73e-14 74.3
MS.gene028134.t1 MTR_6g015315 26.265 415 255 18 45 424 30 428 3.31e-13 71.6
MS.gene028134.t1 MTR_6g053810 24.138 348 189 13 56 355 48 368 5.32e-13 71.2
MS.gene028134.t1 MTR_8g079710 24.641 418 253 22 18 400 4 394 1.23e-12 70.1
MS.gene028134.t1 MTR_2g036780 27.642 246 155 10 40 272 34 269 1.41e-12 69.7
MS.gene028134.t1 MTR_4g094832 24.595 370 228 16 103 448 83 425 3.20e-12 68.6
MS.gene028134.t1 MTR_3g465880 24.194 310 194 9 74 355 68 364 5.12e-12 68.2
MS.gene028134.t1 MTR_1g094790 25.581 387 209 20 56 400 42 391 6.77e-12 67.8
MS.gene028134.t1 MTR_2g015430 21.053 342 240 12 74 400 58 384 1.09e-11 67.0
MS.gene028134.t1 MTR_7g014250 23.821 424 267 19 41 424 26 433 1.78e-11 66.2
MS.gene028134.t1 MTR_7g014330 23.113 424 270 19 41 424 26 433 5.98e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028134.t1 AT1G31490 59.417 446 171 8 16 458 4 442 0.0 563
MS.gene028134.t1 AT1G78990 39.637 386 219 11 53 430 46 425 7.75e-79 252
MS.gene028134.t1 AT5G16410 37.500 440 253 15 3 430 22 451 1.05e-75 245
MS.gene028134.t1 AT1G32910 37.045 440 253 14 5 430 1 430 2.31e-73 238
MS.gene028134.t1 AT5G41040 27.851 377 238 12 41 401 38 396 9.41e-28 115
MS.gene028134.t1 AT5G41040 27.851 377 238 12 41 401 54 412 1.26e-27 115
MS.gene028134.t1 AT1G27620 25.907 386 245 13 33 400 22 384 1.07e-23 103
MS.gene028134.t1 AT3G48720 25.758 396 220 15 41 401 30 386 2.52e-23 102
MS.gene028134.t1 AT2G19070 26.500 400 226 13 48 397 20 401 3.56e-23 102
MS.gene028134.t1 AT3G26040 25.785 446 257 15 17 428 2 407 6.10e-23 101
MS.gene028134.t1 AT1G03390 26.531 392 241 19 35 400 37 407 9.23e-23 100
MS.gene028134.t1 AT5G48930 25.729 377 250 12 42 401 25 388 2.19e-22 99.8
MS.gene028134.t1 AT3G30280 25.882 425 261 20 14 411 2 399 2.09e-21 96.7
MS.gene028134.t1 AT5G57840 25.970 335 225 11 41 360 24 350 2.17e-21 96.7
MS.gene028134.t1 AT1G24430 25.120 418 265 15 27 428 18 403 2.94e-21 96.3
MS.gene028134.t1 AT5G63560 24.627 402 267 13 14 401 3 382 4.94e-21 95.5
MS.gene028134.t1 AT5G47950 23.653 427 283 16 14 428 2 397 9.13e-21 94.7
MS.gene028134.t1 AT5G42830 23.113 424 276 15 58 453 48 449 2.92e-20 93.2
MS.gene028134.t1 AT5G38130 24.729 461 298 16 17 449 23 462 3.40e-20 93.2
MS.gene028134.t1 AT3G03480 25.581 430 267 20 33 436 32 434 2.10e-19 90.9
MS.gene028134.t1 AT3G50270 23.871 465 287 20 18 445 6 440 4.67e-19 89.7
MS.gene028134.t1 AT2G40230 24.873 394 251 15 35 404 20 392 1.11e-18 88.6
MS.gene028134.t1 AT1G24420 29.091 330 192 15 18 327 5 312 1.22e-17 85.5
MS.gene028134.t1 AT1G24420 29.091 330 192 15 18 327 5 312 1.22e-17 85.5
MS.gene028134.t1 AT4G15390 24.390 410 267 16 12 400 2 389 1.27e-17 85.5
MS.gene028134.t1 AT4G15390 24.390 410 267 16 12 400 2 389 1.27e-17 85.5
MS.gene028134.t1 AT1G65450 23.545 378 253 15 41 399 40 400 2.77e-17 84.3
MS.gene028134.t1 AT5G67150 24.883 426 245 21 17 400 4 396 3.14e-17 84.3
MS.gene028134.t1 AT3G50280 25.359 418 251 21 17 400 3 393 5.33e-17 83.6
MS.gene028134.t1 AT4G31910 25.974 308 191 12 25 297 11 316 1.31e-15 79.3
MS.gene028134.t1 AT5G67160 24.289 457 288 17 17 448 4 427 2.18e-15 78.6
MS.gene028134.t1 AT5G07860 20.494 405 278 11 68 451 66 447 1.60e-14 75.9
MS.gene028134.t1 AT1G28680 22.674 344 231 10 74 400 62 387 8.58e-14 73.6
MS.gene028134.t1 AT5G07850 23.028 469 309 17 17 454 7 454 5.63e-13 71.2
MS.gene028134.t1 AT1G28680 22.442 303 209 8 74 360 62 354 4.06e-12 68.2

Find 79 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAACTTGCTTCTGTTAA+TGG 0.124269 5.3:+16152435 MS.gene028134:CDS
TCTCAAATACCAACCTTATT+TGG 0.132764 5.3:+16152302 MS.gene028134:CDS
GAGAGATTAATCACTGAAAA+TGG 0.231244 5.3:-16151790 None:intergenic
TCAAATCTTGATCTACTTTC+AGG 0.243930 5.3:+16151811 MS.gene028134:CDS
CATCGGTAATGTATTGTCTT+TGG 0.264157 5.3:+16152611 MS.gene028134:CDS
TCCTGTTGCTGAAGTTGATT+TGG 0.266026 5.3:+16152848 MS.gene028134:CDS
GGATTTGGAAGTCCTTTGTT+AGG 0.289603 5.3:+16152870 MS.gene028134:CDS
AGCTTCAATCTTTGTTCTCT+TGG 0.297597 5.3:-16152460 None:intergenic
TTCAAAGCATCTACTAATTC+AGG 0.309519 5.3:-16152993 None:intergenic
CCTGTCTTAGTGGTTTCTTC+AGG 0.314491 5.3:+16152816 MS.gene028134:CDS
AGATTTCCCTTTACAAATTC+AGG 0.320821 5.3:+16152113 MS.gene028134:CDS
TAGAAATACAAGTATGTAAC+TGG 0.322652 5.3:-16151841 None:intergenic
TTAGGTGATGCTACTTCATT+TGG 0.333118 5.3:+16152189 MS.gene028134:CDS
TGAATTTGTAAAGGGAAATC+TGG 0.334806 5.3:-16152111 None:intergenic
AATCATTACTATCCCTTTGC+TGG 0.336191 5.3:+16151934 MS.gene028134:CDS
TTGATTGGATTGAGTTTCAT+AGG 0.350449 5.3:+16152748 MS.gene028134:CDS
TTAATCACTGAAAATGGCTT+TGG 0.352525 5.3:-16151784 None:intergenic
GATTTGAAGTTACCTAGTTT+TGG 0.354372 5.3:-16151871 None:intergenic
ATGCTTGCAAAGGCGGTGTT+AGG 0.354998 5.3:+16152780 MS.gene028134:CDS
CCTGTTGCTGAAGTTGATTT+GGG 0.355334 5.3:+16152849 MS.gene028134:CDS
ATTACTTGGATTTGATTGAT+TGG 0.362571 5.3:+16152733 MS.gene028134:CDS
CTGTTACCTGAATTTGTAAA+GGG 0.364202 5.3:-16152119 None:intergenic
GCTGAAGTTGATTTGGGATT+TGG 0.368062 5.3:+16152855 MS.gene028134:CDS
AATGTATTGTCTTTGGCTTT+CGG 0.391960 5.3:+16152618 MS.gene028134:CDS
AAGCTTTCTCTGCATATGTA+TGG 0.403699 5.3:+16152478 MS.gene028134:CDS
GTGGTAAGGGTGATGGTTCA+TGG 0.408074 5.3:+16152949 MS.gene028134:CDS
CCCAAATCAACTTCAGCAAC+AGG 0.408762 5.3:-16152849 None:intergenic
TTGGTAAATTCATTTCATCT+TGG 0.434237 5.3:+16152208 MS.gene028134:CDS
TGAATTAGTAGATGCTTTGA+AGG 0.434332 5.3:+16152995 MS.gene028134:CDS
TCTGTTACCTGAATTTGTAA+AGG 0.444738 5.3:-16152120 None:intergenic
TTGTTGTCACAAATAATTTG+AGG 0.448389 5.3:-16151988 None:intergenic
TAGTAATGAGGAACATTACT+TGG 0.453243 5.3:+16152719 MS.gene028134:CDS
AAGATGGGTTGGTTAGTAGA+TGG 0.454588 5.3:+16152546 MS.gene028134:CDS
GAATCAAAGGCAAAGTGGTA+AGG 0.464027 5.3:+16152935 MS.gene028134:CDS
TGGTTCGGTATGCATGATAA+CGG 0.476676 5.3:-16152252 None:intergenic
AACACCAACCCTTTCAATTG+AGG 0.478455 5.3:-16152904 None:intergenic
GATTTGGAAGTCCTTTGTTA+GGG 0.486362 5.3:+16152871 MS.gene028134:CDS
ATTAAGTATTTGTGCTAGAG+AGG 0.490459 5.3:-16151914 None:intergenic
CTTTGGCTTTCGGTGAGGCA+AGG 0.495189 5.3:+16152628 MS.gene028134:CDS
TGGATTGAGTTTCATAGGCC+AGG 0.499721 5.3:+16152753 MS.gene028134:CDS
TGAACAATGTGGCCAGCAAA+GGG 0.502111 5.3:-16151946 None:intergenic
AGATGATGGACCTGTCTTAG+TGG 0.507067 5.3:+16152806 MS.gene028134:CDS
TTGAACAATGTGGCCAGCAA+AGG 0.507323 5.3:-16151947 None:intergenic
ACAAATAATTTGAGGTTCGT+CGG 0.512193 5.3:-16151980 None:intergenic
ACAAGTATGTAACTGGAAAA+CGG 0.512372 5.3:-16151834 None:intergenic
CTGAAGATGAGAAGCAGTCA+TGG 0.513807 5.3:-16153036 None:intergenic
AATCAAAGGCAAAGTGGTAA+GGG 0.520980 5.3:+16152936 MS.gene028134:CDS
AGGTTTGATGCTTGCAAAGG+CGG 0.529986 5.3:+16152773 MS.gene028134:CDS
AGTTCATGATGCAATTTCAA+AGG 0.532962 5.3:+16152695 MS.gene028134:CDS
AATTTCAAAGGTTAGTAATG+AGG 0.533878 5.3:+16152707 MS.gene028134:CDS
CAGTGTATACCTCAATTGAA+AGG 0.543563 5.3:+16152895 MS.gene028134:CDS
GATGGAAGAGAAAGAATGAG+TGG 0.544115 5.3:+16152564 MS.gene028134:CDS
GTAACAGAATTTACTTGTGG+TGG 0.546469 5.3:+16152135 MS.gene028134:CDS
AAATTTGATCCAAATAAGGT+TGG 0.546882 5.3:-16152311 None:intergenic
GGCAAGGATTCAAGAATTGA+AGG 0.552829 5.3:+16152644 MS.gene028134:CDS
GCCTTTGCAAGCATCAAACC+TGG 0.556923 5.3:-16152771 None:intergenic
GTGTTGGTTATATGAATCAA+AGG 0.560599 5.3:+16152922 MS.gene028134:CDS
AGTGTATACCTCAATTGAAA+GGG 0.563977 5.3:+16152896 MS.gene028134:CDS
TCAATTGAAAGGGTTGGTGT+TGG 0.564088 5.3:+16152906 MS.gene028134:CDS
CCTGAAGAAACCACTAAGAC+AGG 0.567163 5.3:-16152816 None:intergenic
GGACTGTTTCTGCTATCTTG+TGG 0.568637 5.3:+16152970 MS.gene028134:CDS
CAAATTATTTGTGACAACAA+TGG 0.572056 5.3:+16151991 MS.gene028134:CDS
TAGTGGTTTCTTCAGGACAA+AGG 0.574621 5.3:+16152823 MS.gene028134:CDS
TGAGAGGCGAACGCGCGCAA+AGG 0.590576 5.3:-16152284 None:intergenic
AGGATTCAAGAATTGAAGGA+AGG 0.590702 5.3:+16152648 MS.gene028134:CDS
CTGCAGCATGTTAATACTCG+AGG 0.591931 5.3:-16152415 None:intergenic
GCCAGGTTTGATGCTTGCAA+AGG 0.594613 5.3:+16152770 MS.gene028134:CDS
TATACCTCAATTGAAAGGGT+TGG 0.598377 5.3:+16152900 MS.gene028134:CDS
TGAAGATGAGAAGCAGTCAT+GGG 0.600000 5.3:-16153035 None:intergenic
AGGCAAAGTGGTAAGGGTGA+TGG 0.604002 5.3:+16152942 MS.gene028134:CDS
AAATAAGGTTGGTATTTGAG+AGG 0.609809 5.3:-16152300 None:intergenic
GAAGGTTCATTATCAGACAT+AGG 0.615379 5.3:+16152666 MS.gene028134:CDS
GCGGTGTTAGGACAAGATGA+TGG 0.622280 5.3:+16152792 MS.gene028134:CDS
ATTGTCTTTGGCTTTCGGTG+AGG 0.622701 5.3:+16152623 MS.gene028134:CDS
CAGGTAACAGAATTTACTTG+TGG 0.623975 5.3:+16152132 MS.gene028134:CDS
AATTCAATGTCGAATTACAT+CGG 0.628778 5.3:+16152594 MS.gene028134:CDS
TATATGAATCAAAGGCAAAG+TGG 0.632585 5.3:+16152930 MS.gene028134:CDS
GTATACACTGTCCCTAACAA+AGG 0.661728 5.3:-16152882 None:intergenic
ATGGAAGAAAATGATAAACA+CGG 0.726903 5.3:+16152497 MS.gene028134:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATTTGATCCAAATA+AGG - chr5.3:16152318-16152337 None:intergenic 15.0%
!! TAAAAATCTAAATTACTTAG+TGG - chr5.3:16152048-16152067 None:intergenic 15.0%
!!! TTCAAATTTTTCATATTCTT+GGG - chr5.3:16151719-16151738 None:intergenic 15.0%
!!! CTTCAAATTTTTCATATTCT+TGG - chr5.3:16151720-16151739 None:intergenic 20.0%
!!! TTTTATGATTTTGAACAATG+TGG - chr5.3:16151960-16151979 None:intergenic 20.0%
! AAATTTGATCCAAATAAGGT+TGG - chr5.3:16152314-16152333 None:intergenic 25.0%
! AATTCAATGTCGAATTACAT+CGG + chr5.3:16152594-16152613 MS.gene028134:CDS 25.0%
! AATTTCAAAGGTTAGTAATG+AGG + chr5.3:16152707-16152726 MS.gene028134:CDS 25.0%
! ACTTCATAAAAAATGCAAGA+TGG + chr5.3:16152530-16152549 MS.gene028134:CDS 25.0%
! ATGGAAGAAAATGATAAACA+CGG + chr5.3:16152497-16152516 MS.gene028134:CDS 25.0%
! ATTACTTGGATTTGATTGAT+TGG + chr5.3:16152733-16152752 MS.gene028134:CDS 25.0%
! CAAATTATTTGTGACAACAA+TGG + chr5.3:16151991-16152010 MS.gene028134:CDS 25.0%
! CTTCATAAAAAATGCAAGAT+GGG + chr5.3:16152531-16152550 MS.gene028134:CDS 25.0%
! TAGAAATACAAGTATGTAAC+TGG - chr5.3:16151844-16151863 None:intergenic 25.0%
! TTCTACAAAAAACCAAAACT+AGG + chr5.3:16151859-16151878 MS.gene028134:CDS 25.0%
! TTGGTAAATTCATTTCATCT+TGG + chr5.3:16152208-16152227 MS.gene028134:CDS 25.0%
! TTGTTGTCACAAATAATTTG+AGG - chr5.3:16151991-16152010 None:intergenic 25.0%
!! AATTTTTATCAAATGCACCA+TGG + chr5.3:16152329-16152348 MS.gene028134:CDS 25.0%
!!! TTTACTTTTGATCATGCATT+AGG + chr5.3:16152171-16152190 MS.gene028134:CDS 25.0%
ACAAGTATGTAACTGGAAAA+CGG - chr5.3:16151837-16151856 None:intergenic 30.0%
AGATTTCCCTTTACAAATTC+AGG + chr5.3:16152113-16152132 MS.gene028134:CDS 30.0%
AGTGTATACCTCAATTGAAA+GGG + chr5.3:16152896-16152915 MS.gene028134:CDS 30.0%
AGTTCATGATGCAATTTCAA+AGG + chr5.3:16152695-16152714 MS.gene028134:CDS 30.0%
ATAAAAAATGCAAGATGGGT+TGG + chr5.3:16152535-16152554 MS.gene028134:CDS 30.0%
ATTAAGTATTTGTGCTAGAG+AGG - chr5.3:16151917-16151936 None:intergenic 30.0%
CTGTTACCTGAATTTGTAAA+GGG - chr5.3:16152122-16152141 None:intergenic 30.0%
GAGAGATTAATCACTGAAAA+TGG - chr5.3:16151793-16151812 None:intergenic 30.0%
TAGTAATGAGGAACATTACT+TGG + chr5.3:16152719-16152738 MS.gene028134:CDS 30.0%
TATATGAATCAAAGGCAAAG+TGG + chr5.3:16152930-16152949 MS.gene028134:CDS 30.0%
TCAAATCTTGATCTACTTTC+AGG + chr5.3:16151811-16151830 MS.gene028134:CDS 30.0%
TCTCAAATACCAACCTTATT+TGG + chr5.3:16152302-16152321 MS.gene028134:CDS 30.0%
TCTGTTACCTGAATTTGTAA+AGG - chr5.3:16152123-16152142 None:intergenic 30.0%
TGAATTAGTAGATGCTTTGA+AGG + chr5.3:16152995-16153014 MS.gene028134:CDS 30.0%
TGAATTTGTAAAGGGAAATC+TGG - chr5.3:16152114-16152133 None:intergenic 30.0%
TTAATCACTGAAAATGGCTT+TGG - chr5.3:16151787-16151806 None:intergenic 30.0%
TTGATTGGATTGAGTTTCAT+AGG + chr5.3:16152748-16152767 MS.gene028134:CDS 30.0%
! ACAAATAATTTGAGGTTCGT+CGG - chr5.3:16151983-16152002 None:intergenic 30.0%
! CAAAGGTTTCTTTTATGGTT+CGG - chr5.3:16152270-16152289 None:intergenic 30.0%
! CATATTTTGTATCTCTTCCA+TGG - chr5.3:16152349-16152368 None:intergenic 30.0%
! TTCAAAGCATCTACTAATTC+AGG - chr5.3:16152996-16153015 None:intergenic 30.0%
!! AAATAAGGTTGGTATTTGAG+AGG - chr5.3:16152303-16152322 None:intergenic 30.0%
!! AATGTATTGTCTTTGGCTTT+CGG + chr5.3:16152618-16152637 MS.gene028134:CDS 30.0%
!! GATTTGAAGTTACCTAGTTT+TGG - chr5.3:16151874-16151893 None:intergenic 30.0%
!! GTGTTGGTTATATGAATCAA+AGG + chr5.3:16152922-16152941 MS.gene028134:CDS 30.0%
AAGCTTTCTCTGCATATGTA+TGG + chr5.3:16152478-16152497 MS.gene028134:CDS 35.0%
AATCAAAGGCAAAGTGGTAA+GGG + chr5.3:16152936-16152955 MS.gene028134:CDS 35.0%
AATCATTACTATCCCTTTGC+TGG + chr5.3:16151934-16151953 MS.gene028134:CDS 35.0%
AGCTTCAATCTTTGTTCTCT+TGG - chr5.3:16152463-16152482 None:intergenic 35.0%
AGGATTCAAGAATTGAAGGA+AGG + chr5.3:16152648-16152667 MS.gene028134:CDS 35.0%
CAGGTAACAGAATTTACTTG+TGG + chr5.3:16152132-16152151 MS.gene028134:CDS 35.0%
CAGTGTATACCTCAATTGAA+AGG + chr5.3:16152895-16152914 MS.gene028134:CDS 35.0%
CATCGGTAATGTATTGTCTT+TGG + chr5.3:16152611-16152630 MS.gene028134:CDS 35.0%
GAAGGTTCATTATCAGACAT+AGG + chr5.3:16152666-16152685 MS.gene028134:CDS 35.0%
GATTTGGAAGTCCTTTGTTA+GGG + chr5.3:16152871-16152890 MS.gene028134:CDS 35.0%
GTAACAGAATTTACTTGTGG+TGG + chr5.3:16152135-16152154 MS.gene028134:CDS 35.0%
TATACCTCAATTGAAAGGGT+TGG + chr5.3:16152900-16152919 MS.gene028134:CDS 35.0%
! CAGAAACTTGCTTCTGTTAA+TGG + chr5.3:16152435-16152454 MS.gene028134:CDS 35.0%
! TTAGGTGATGCTACTTCATT+TGG + chr5.3:16152189-16152208 MS.gene028134:CDS 35.0%
AACACCAACCCTTTCAATTG+AGG - chr5.3:16152907-16152926 None:intergenic 40.0%
GAATCAAAGGCAAAGTGGTA+AGG + chr5.3:16152935-16152954 MS.gene028134:CDS 40.0%
GATGGAAGAGAAAGAATGAG+TGG + chr5.3:16152564-16152583 MS.gene028134:CDS 40.0%
GGATTTGGAAGTCCTTTGTT+AGG + chr5.3:16152870-16152889 MS.gene028134:CDS 40.0%
GGCAAGGATTCAAGAATTGA+AGG + chr5.3:16152644-16152663 MS.gene028134:CDS 40.0%
GTATACACTGTCCCTAACAA+AGG - chr5.3:16152885-16152904 None:intergenic 40.0%
TAGTGGTTTCTTCAGGACAA+AGG + chr5.3:16152823-16152842 MS.gene028134:CDS 40.0%
TGAAGATGAGAAGCAGTCAT+GGG - chr5.3:16153038-16153057 None:intergenic 40.0%
! CCTGTTGCTGAAGTTGATTT+GGG + chr5.3:16152849-16152868 MS.gene028134:CDS 40.0%
! TCCTGTTGCTGAAGTTGATT+TGG + chr5.3:16152848-16152867 MS.gene028134:CDS 40.0%
!! AAGATGGGTTGGTTAGTAGA+TGG + chr5.3:16152546-16152565 MS.gene028134:CDS 40.0%
!! GCTGAAGTTGATTTGGGATT+TGG + chr5.3:16152855-16152874 MS.gene028134:CDS 40.0%
!! TCAATTGAAAGGGTTGGTGT+TGG + chr5.3:16152906-16152925 MS.gene028134:CDS 40.0%
!! TGGTTCGGTATGCATGATAA+CGG - chr5.3:16152255-16152274 None:intergenic 40.0%
AGGTTTGATGCTTGCAAAGG+CGG + chr5.3:16152773-16152792 MS.gene028134:CDS 45.0%
CCCAAATCAACTTCAGCAAC+AGG - chr5.3:16152852-16152871 None:intergenic 45.0%
CCTGAAGAAACCACTAAGAC+AGG - chr5.3:16152819-16152838 None:intergenic 45.0%
CCTGTCTTAGTGGTTTCTTC+AGG + chr5.3:16152816-16152835 MS.gene028134:CDS 45.0%
CTGAAGATGAGAAGCAGTCA+TGG - chr5.3:16153039-16153058 None:intergenic 45.0%
CTGCAGCATGTTAATACTCG+AGG - chr5.3:16152418-16152437 None:intergenic 45.0%
TGAACAATGTGGCCAGCAAA+GGG - chr5.3:16151949-16151968 None:intergenic 45.0%
TGGATTGAGTTTCATAGGCC+AGG + chr5.3:16152753-16152772 MS.gene028134:CDS 45.0%
TTGAACAATGTGGCCAGCAA+AGG - chr5.3:16151950-16151969 None:intergenic 45.0%
! GCAGTCATGGGCTGAAAAAT+TGG - chr5.3:16153026-16153045 None:intergenic 45.0%
! GCGCGCAAAGGTTTCTTTTA+TGG - chr5.3:16152275-16152294 None:intergenic 45.0%
!! AGATGATGGACCTGTCTTAG+TGG + chr5.3:16152806-16152825 MS.gene028134:CDS 45.0%
!! ATTGTCTTTGGCTTTCGGTG+AGG + chr5.3:16152623-16152642 MS.gene028134:CDS 45.0%
!! GGACTGTTTCTGCTATCTTG+TGG + chr5.3:16152970-16152989 MS.gene028134:CDS 45.0%
AGGCAAAGTGGTAAGGGTGA+TGG + chr5.3:16152942-16152961 MS.gene028134:CDS 50.0%
ATGCTTGCAAAGGCGGTGTT+AGG + chr5.3:16152780-16152799 MS.gene028134:CDS 50.0%
GCCAGGTTTGATGCTTGCAA+AGG + chr5.3:16152770-16152789 MS.gene028134:CDS 50.0%
GCCTTTGCAAGCATCAAACC+TGG - chr5.3:16152774-16152793 None:intergenic 50.0%
! GCGGTGTTAGGACAAGATGA+TGG + chr5.3:16152792-16152811 MS.gene028134:CDS 50.0%
! GTGGTAAGGGTGATGGTTCA+TGG + chr5.3:16152949-16152968 MS.gene028134:CDS 50.0%
!! CTTTGGCTTTCGGTGAGGCA+AGG + chr5.3:16152628-16152647 MS.gene028134:CDS 55.0%
TGAGAGGCGAACGCGCGCAA+AGG - chr5.3:16152287-16152306 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 16151688 16153064 16151688 ID=MS.gene028134
chr5.3 mRNA 16151688 16153064 16151688 ID=MS.gene028134.t1;Parent=MS.gene028134
chr5.3 exon 16151688 16153064 16151688 ID=MS.gene028134.t1.exon1;Parent=MS.gene028134.t1
chr5.3 CDS 16151688 16153064 16151688 ID=cds.MS.gene028134.t1;Parent=MS.gene028134.t1
Gene Sequence

>MS.gene028134

ATGTCACATATTCTCAATGATATTGCATCCCAAGAATATGAAAAATTTGAAGTAAAGTTCATTAAGAAAAGTGTTGTAAAAGCTCTTAATCATTTTCCAAAGCCATTTTCAGTGATTAATCTCTCAAATCTTGATCTACTTTCAGGCCGTTTTCCAGTTACATACTTGTATTTCTACAAAAAACCAAAACTAGGTAACTTCAAATCTTTTGTTAACAATCTTAAAACCTCTCTAGCACAAATACTTAATCATTACTATCCCTTTGCTGGCCACATTGTTCAAAATCATAAAACCGACGAACCTCAAATTATTTGTGACAACAATGGTTCATTAGTCATTGAAGCTCAAGTAAATATTCCACTAAGTAATTTAGATTTTTATAATCTCAATGAAACTCTTGAAGAAAAGATAGTCTTAGTTGAACCAGATTTCCCTTTACAAATTCAGGTAACAGAATTTACTTGTGGTGGAATTTCAATAGCATTTACTTTTGATCATGCATTAGGTGATGCTACTTCATTTGGTAAATTCATTTCATCTTGGTGTGAAATAGCACAAAATAAACCGTTATCATGCATACCGAACCATAAAAGAAACCTTTGCGCGCGTTCGCCTCTCAAATACCAACCTTATTTGGATCAAATTTTTATCAAATGCACCATGGAAGAGATACAAAATATGAAAATAAATAAAAATATTTCGCTTAAACGTCTTTATCATATCGATGCCTCGAGTATTAACATGCTGCAGAAACTTGCTTCTGTTAATGGTACCAAGAGAACAAAGATTGAAGCTTTCTCTGCATATGTATGGAAGAAAATGATAAACACGGTTGATCAAAAACTTCATAAAAAATGCAAGATGGGTTGGTTAGTAGATGGAAGAGAAAGAATGAGTGGAAGCAAAAATTCAATGTCGAATTACATCGGTAATGTATTGTCTTTGGCTTTCGGTGAGGCAAGGATTCAAGAATTGAAGGAAGGTTCATTATCAGACATAGGTGAAATAGTTCATGATGCAATTTCAAAGGTTAGTAATGAGGAACATTACTTGGATTTGATTGATTGGATTGAGTTTCATAGGCCAGGTTTGATGCTTGCAAAGGCGGTGTTAGGACAAGATGATGGACCTGTCTTAGTGGTTTCTTCAGGACAAAGGTTTCCTGTTGCTGAAGTTGATTTGGGATTTGGAAGTCCTTTGTTAGGGACAGTGTATACCTCAATTGAAAGGGTTGGTGTTGGTTATATGAATCAAAGGCAAAGTGGTAAGGGTGATGGTTCATGGACTGTTTCTGCTATCTTGTGGCCTGAATTAGTAGATGCTTTGAAGGATGATCCAATTTTTCAGCCCATGACTGCTTCTCATCTTCAGCTTTAG
Protein sequence

>MS.gene028134.t1

MSHILNDIASQEYEKFEVKFIKKSVVKALNHFPKPFSVINLSNLDLLSGRFPVTYLYFYKKPKLGNFKSFVNNLKTSLAQILNHYYPFAGHIVQNHKTDEPQIICDNNGSLVIEAQVNIPLSNLDFYNLNETLEEKIVLVEPDFPLQIQVTEFTCGGISIAFTFDHALGDATSFGKFISSWCEIAQNKPLSCIPNHKRNLCARSPLKYQPYLDQIFIKCTMEEIQNMKINKNISLKRLYHIDASSINMLQKLASVNGTKRTKIEAFSAYVWKKMINTVDQKLHKKCKMGWLVDGRERMSGSKNSMSNYIGNVLSLAFGEARIQELKEGSLSDIGEIVHDAISKVSNEEHYLDLIDWIEFHRPGLMLAKAVLGQDDGPVLVVSSGQRFPVAEVDLGFGSPLLGTVYTSIERVGVGYMNQRQSGKGDGSWTVSAILWPELVDALKDDPIFQPMTASHLQL