AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48027

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48027.t1 MTR_5g090880 93.658 473 30 0 1 473 1 473 0.0 902
MS.gene48027.t1 MTR_5g090830 80.169 474 90 3 1 473 1 471 0.0 793
MS.gene48027.t1 MTR_5g090810 78.692 474 97 3 1 473 1 471 0.0 780
MS.gene48027.t1 MTR_3g450710 62.630 479 160 7 1 471 1 468 0.0 580
MS.gene48027.t1 MTR_3g450690 61.042 480 170 6 1 471 1 472 0.0 580
MS.gene48027.t1 MTR_5g026320 35.394 469 272 13 7 471 15 456 3.78e-74 241
MS.gene48027.t1 MTR_8g075610 25.442 452 287 15 17 456 1 414 4.30e-36 138
MS.gene48027.t1 MTR_4g007540 26.362 459 284 16 10 452 3 423 8.42e-36 138
MS.gene48027.t1 MTR_2g064495 25.974 462 289 16 10 456 3 426 1.27e-35 137
MS.gene48027.t1 MTR_2g105330 25.974 462 289 16 10 456 3 426 2.29e-35 136
MS.gene48027.t1 MTR_8g096530 26.196 439 274 16 31 459 28 426 2.27e-30 122
MS.gene48027.t1 MTR_3g098980 26.904 394 239 14 31 414 35 389 5.51e-28 115
MS.gene48027.t1 MTR_1g040215 26.368 402 233 13 64 437 61 427 2.39e-27 114
MS.gene48027.t1 MTR_3g049150 24.879 414 273 14 60 456 48 440 3.40e-27 114
MS.gene48027.t1 MTR_7g027175 26.241 423 263 17 4 415 9 393 2.14e-26 111
MS.gene48027.t1 MTR_7g114560 25.063 399 255 12 64 450 62 428 7.76e-26 110
MS.gene48027.t1 MTR_7g085990 26.562 384 232 15 61 414 49 412 2.35e-25 108
MS.gene48027.t1 MTR_1g040175 25.123 406 245 13 64 443 61 433 3.45e-25 108
MS.gene48027.t1 MTR_7g114550 25.564 399 247 14 67 450 65 428 6.07e-25 107
MS.gene48027.t1 MTR_4g127650 23.632 457 303 13 28 466 28 456 1.38e-24 106
MS.gene48027.t1 MTR_7g086000 26.133 375 223 14 67 413 60 408 6.00e-24 104
MS.gene48027.t1 MTR_4g097090 25.000 456 299 16 28 466 27 456 2.09e-23 102
MS.gene48027.t1 MTR_7g114490 24.634 410 261 14 64 459 62 437 4.67e-23 102
MS.gene48027.t1 MTR_5g076700 25.926 405 239 17 67 450 65 429 5.27e-23 101
MS.gene48027.t1 MTR_7g114470 25.505 396 242 15 64 435 63 429 5.71e-23 101
MS.gene48027.t1 MTR_1g081660 24.566 403 255 17 64 452 70 437 8.85e-23 100
MS.gene48027.t1 MTR_3g026950 24.289 457 304 12 10 447 2 435 1.27e-21 97.4
MS.gene48027.t1 MTR_7g114520 26.648 364 224 14 64 416 63 394 2.09e-21 97.1
MS.gene48027.t1 MTR_7g085930 25.197 381 241 13 61 414 53 416 4.22e-21 96.3
MS.gene48027.t1 MTR_7g084940 26.761 426 249 18 6 413 12 392 3.97e-19 90.1
MS.gene48027.t1 MTR_1g040320 23.284 408 214 14 64 443 61 397 2.13e-18 87.8
MS.gene48027.t1 MTR_1g053315 23.284 408 254 16 29 414 40 410 4.52e-18 87.0
MS.gene48027.t1 MTR_1g040195 24.796 367 215 14 97 437 71 402 5.82e-18 86.3
MS.gene48027.t1 MTR_3g465990 25.304 411 254 15 49 440 50 426 1.55e-17 85.1
MS.gene48027.t1 MTR_7g084910 25.380 461 256 20 7 435 14 418 3.02e-17 84.3
MS.gene48027.t1 MTR_3g465980 25.519 337 211 10 49 369 50 362 1.57e-16 82.0
MS.gene48027.t1 MTR_3g086020 23.944 426 254 20 6 417 1 370 2.85e-16 81.3
MS.gene48027.t1 MTR_1g040315 26.457 223 141 6 64 278 61 268 7.63e-16 79.7
MS.gene48027.t1 MTR_4g055170 23.716 409 251 13 67 449 69 442 9.28e-16 79.7
MS.gene48027.t1 MTR_8g079980 23.836 365 219 11 67 414 59 381 1.40e-15 79.0
MS.gene48027.t1 MTR_3g101900 23.885 381 232 13 67 425 66 410 5.35e-15 77.4
MS.gene48027.t1 MTR_2g015430 23.210 405 267 16 21 415 15 385 1.57e-14 75.9
MS.gene48027.t1 MTR_8g467580 23.853 436 249 20 10 414 3 386 1.93e-14 75.5
MS.gene48027.t1 MTR_7g056087 23.176 466 296 18 10 453 4 429 4.52e-14 74.3
MS.gene48027.t1 MTR_3g101930 24.403 377 221 14 62 412 65 403 5.84e-14 74.3
MS.gene48027.t1 MTR_4g022720 25.815 368 218 14 67 414 55 387 1.45e-13 72.8
MS.gene48027.t1 MTR_3g112180 23.544 395 229 14 67 429 61 414 2.08e-13 72.4
MS.gene48027.t1 MTR_3g101960 22.911 371 228 11 63 412 64 397 4.63e-13 71.2
MS.gene48027.t1 MTR_8g079950 22.306 399 253 15 31 414 26 382 8.86e-13 70.5
MS.gene48027.t1 MTR_4g022290 23.873 377 216 15 67 414 60 394 1.49e-12 69.7
MS.gene48027.t1 MTR_6g053810 24.695 328 183 13 67 365 71 363 5.17e-12 68.2
MS.gene48027.t1 MTR_4g094828 22.717 449 272 17 36 455 37 439 7.17e-12 67.8
MS.gene48027.t1 MTR_3g465930 23.547 327 202 9 67 372 69 368 7.47e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48027.t1 AT1G78990 54.292 466 188 9 8 471 11 453 2.32e-162 467
MS.gene48027.t1 AT1G32910 54.797 469 186 9 8 471 11 458 6.97e-161 463
MS.gene48027.t1 AT5G16410 50.633 474 196 12 8 471 34 479 4.38e-151 439
MS.gene48027.t1 AT1G31490 35.639 477 254 15 8 471 4 440 7.17e-76 245
MS.gene48027.t1 AT5G48930 26.464 461 289 16 10 456 3 427 1.54e-34 134
MS.gene48027.t1 AT5G41040 26.076 395 246 13 31 415 38 396 1.50e-27 114
MS.gene48027.t1 AT5G41040 26.076 395 246 13 31 415 54 412 1.97e-27 114
MS.gene48027.t1 AT5G57840 25.270 463 288 15 10 452 3 427 7.95e-27 112
MS.gene48027.t1 AT3G26040 25.463 432 241 19 10 414 3 380 4.52e-25 107
MS.gene48027.t1 AT5G17540 25.325 462 271 19 4 440 3 415 2.39e-24 105
MS.gene48027.t1 AT5G63560 25.893 448 252 18 8 425 5 402 1.22e-23 103
MS.gene48027.t1 AT3G03480 25.000 464 274 17 6 437 15 436 3.92e-23 102
MS.gene48027.t1 AT3G48720 24.784 464 299 16 3 456 2 425 1.06e-22 100
MS.gene48027.t1 AT1G65450 26.111 360 222 13 67 413 72 400 2.20e-21 97.1
MS.gene48027.t1 AT1G28680 27.475 404 242 20 26 415 22 388 4.35e-21 95.9
MS.gene48027.t1 AT1G24430 26.492 419 247 18 10 414 5 376 1.04e-20 94.7
MS.gene48027.t1 AT2G40230 25.432 405 243 14 62 448 54 417 1.18e-20 94.7
MS.gene48027.t1 AT2G19070 22.689 476 321 15 10 466 4 451 1.80e-20 94.4
MS.gene48027.t1 AT5G67160 25.814 430 245 19 64 473 53 428 1.10e-19 91.7
MS.gene48027.t1 AT1G27620 22.738 409 253 13 67 456 58 422 2.86e-19 90.5
MS.gene48027.t1 AT3G30280 25.185 405 243 16 27 414 25 386 1.25e-17 85.5
MS.gene48027.t1 AT5G47950 23.060 451 276 15 19 456 17 409 1.03e-15 79.3
MS.gene48027.t1 AT4G15390 26.761 284 154 11 27 292 27 274 4.67e-15 77.4
MS.gene48027.t1 AT4G15390 26.761 284 154 11 27 292 27 274 4.67e-15 77.4
MS.gene48027.t1 AT1G24420 25.176 425 256 15 6 414 1 379 2.60e-14 75.1
MS.gene48027.t1 AT1G24420 25.176 425 256 15 6 414 1 379 2.60e-14 75.1
MS.gene48027.t1 AT5G67150 24.599 374 226 17 63 414 57 396 4.00e-14 74.7
MS.gene48027.t1 AT5G23970 22.748 444 278 16 10 439 3 395 6.27e-14 73.9
MS.gene48027.t1 AT2G25150 24.029 412 270 13 16 414 19 400 2.37e-13 72.4
MS.gene48027.t1 AT5G47980 22.246 472 288 20 6 450 1 420 5.41e-13 71.2
MS.gene48027.t1 AT3G50280 24.028 283 177 10 137 414 14 263 1.57e-12 68.9
MS.gene48027.t1 AT5G42830 22.933 375 236 14 61 415 58 399 5.54e-12 68.2
MS.gene48027.t1 AT3G50300 23.592 373 228 16 64 414 56 393 6.57e-12 67.8
MS.gene48027.t1 AT1G65450 25.191 262 160 10 157 413 6 236 3.17e-11 64.7

Find 128 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAGTCAAAGGTAGATTT+TGG 0.197134 5.2:-76892285 MS.gene48027:CDS
GGATGGAAGGTGGGTGTCTT+GGG 0.229374 5.2:+76892892 None:intergenic
TGCAACAACATTTCCACTAT+TGG 0.271768 5.2:+76892632 None:intergenic
CCGACATTTGTGGTGTCTTC+TGG 0.272565 5.2:-76892318 MS.gene48027:CDS
GAGTTTATGAGTTCGTATTT+TGG 0.286533 5.2:-76892549 MS.gene48027:CDS
ACACCCCACAATAGTGTTAT+TGG 0.294608 5.2:+76893473 None:intergenic
GTTTCTTATTACGTCTTTGC+TGG 0.306691 5.2:-76893423 MS.gene48027:CDS
TCTGATTCGGGCTTGGGTTC+AGG 0.310598 5.2:+76892819 None:intergenic
TTAATATCTGATTCGGGCTT+GGG 0.319670 5.2:+76892813 None:intergenic
TTTAATATCTGATTCGGGCT+TGG 0.321191 5.2:+76892812 None:intergenic
GAAGAGCATCGACCGGTTCC+AGG 0.324397 5.2:-76892384 MS.gene48027:CDS
TGGATGGAAGGTGGGTGTCT+TGG 0.324811 5.2:+76892891 None:intergenic
TAAGGAATACTTGCACTTCA+TGG 0.338830 5.2:-76892145 MS.gene48027:CDS
ATTTCTTGGGACTAATTGAT+TGG 0.344863 5.2:-76892410 MS.gene48027:CDS
AGGATTTATTGCGGAGTTCC+TGG 0.347311 5.2:-76892354 MS.gene48027:CDS
CAAACATGTTTCTTGTATCA+TGG 0.350145 5.2:-76892992 MS.gene48027:CDS
TTCTGCTTTCTTGTGGAAGA+TGG 0.357240 5.2:-76892673 MS.gene48027:CDS
TGCCAATGCCTAGTCCTAAA+AGG 0.369732 5.2:-76892200 MS.gene48027:CDS
ATGAGATTGGTCTAAAGAAA+TGG 0.371643 5.2:+76892109 None:intergenic
AAGAACACACCAATACTAAC+AGG 0.372400 5.2:+76893531 None:intergenic
AAGAAACATGTTTGTAGAAT+AGG 0.374201 5.2:+76893001 None:intergenic
GGACACGAAAGAACATTTCT+TGG 0.384547 5.2:-76892424 MS.gene48027:CDS
TTCCCTTCAATGGTTTCCTC+TGG 0.385000 5.2:+76893288 None:intergenic
TGGAACAAGCTTCCCTTCAA+TGG 0.386738 5.2:+76893278 None:intergenic
AGCTGCAACTACCTCCTCTT+TGG 0.387741 5.2:+76893611 None:intergenic
ACATGGTGTGCTTGCTGTTC+AGG 0.388373 5.2:-76893247 MS.gene48027:intron
ATTCCTTGTGGTGGTGATGC+TGG 0.393172 5.2:-76892231 MS.gene48027:CDS
TTACGTCTTTGCTGGTGAAA+TGG 0.396605 5.2:-76893415 MS.gene48027:CDS
TCGGGCTTGGGTTCAGGTGG+AGG 0.406362 5.2:+76892825 None:intergenic
GTGGGTGTCTTGGGGTCAAA+AGG 0.407163 5.2:+76892901 None:intergenic
CAATAAATCCTTGCCAGCCC+TGG 0.423385 5.2:+76892366 None:intergenic
CGCTCTGTAACCCAACATAA+TGG 0.424141 5.2:+76892471 None:intergenic
TGAGATTGGTCTAAAGAAAT+GGG 0.424402 5.2:+76892110 None:intergenic
ATGAATCCTATGATTACTAT+TGG 0.425230 5.2:-76893501 MS.gene48027:CDS
CTAATCACTAAAATGAGTAT+TGG 0.430164 5.2:-76893660 None:intergenic
TGTTGAAAAGCCATTATGTT+GGG 0.431386 5.2:-76892481 MS.gene48027:CDS
CCAAAATCTACCTTTGACTC+TGG 0.435822 5.2:+76892285 None:intergenic
GCATCGACCGGTTCCAGGGC+TGG 0.439356 5.2:-76892379 MS.gene48027:CDS
ATCCTTGCCAGCCCTGGAAC+CGG 0.444779 5.2:+76892372 None:intergenic
GCTTCCACTATTATTTGTGT+TGG 0.455348 5.2:+76892722 None:intergenic
CCTATGTTCTTGCATTGCCT+TGG 0.455581 5.2:+76893587 None:intergenic
GACACGAAAGAACATTTCTT+GGG 0.462909 5.2:-76892423 MS.gene48027:CDS
ACCACCAATAACACTATTGT+GGG 0.470153 5.2:-76893477 MS.gene48027:CDS
TTTCTTGGGACTAATTGATT+GGG 0.478579 5.2:-76892409 MS.gene48027:CDS
TCTATTAATTTACAACTGTA+GGG 0.479476 5.2:+76892510 None:intergenic
ATGAAGTGCAAGTATTCCTT+AGG 0.482803 5.2:+76892147 None:intergenic
GGCTTGGGTTCAGGTGGAGG+AGG 0.482969 5.2:+76892828 None:intergenic
ATTGCTCGAGCGGGCTATCT+CGG 0.484103 5.2:+76892968 None:intergenic
ATTTCTTTAGACCAATCTCA+TGG 0.487418 5.2:-76892107 MS.gene48027:CDS
GTCCTAAAAGGAATGGTGAT+TGG 0.487820 5.2:-76892188 MS.gene48027:CDS
AGAAGGTAATCCCATGAGAT+TGG 0.494084 5.2:+76892096 None:intergenic
TTCTATTAATTTACAACTGT+AGG 0.494706 5.2:+76892509 None:intergenic
GACCGGTTCCAGGGCTGGCA+AGG 0.497114 5.2:-76892374 MS.gene48027:CDS
CGGTTGTTGCAAAGTAGCTC+AGG 0.499190 5.2:+76893372 None:intergenic
AAACATGTTTCTTGTATCAT+GGG 0.502267 5.2:-76892991 MS.gene48027:CDS
TCAGCCGCTTCAACCAATAG+TGG 0.504854 5.2:-76892645 MS.gene48027:CDS
AGTACATGCGACTTATTAGC+TGG 0.506554 5.2:+76892784 None:intergenic
GGATCTTATCATATTCCTTG+TGG 0.513474 5.2:-76892243 MS.gene48027:CDS
GATGGAAGGTGGGTGTCTTG+GGG 0.526099 5.2:+76892893 None:intergenic
CTAGCCAACACAAATAATAG+TGG 0.526578 5.2:-76892726 MS.gene48027:CDS
CACCACCAATAACACTATTG+TGG 0.528613 5.2:-76893478 MS.gene48027:CDS
GTGAAGGATGGATGGAAGGT+GGG 0.534125 5.2:+76892883 None:intergenic
GTCATAGAGTGAAGGATGGA+TGG 0.535942 5.2:+76892875 None:intergenic
AAGCCAGAGGAAACCATTGA+AGG 0.538348 5.2:-76893291 MS.gene48027:CDS
GATTCGGGCTTGGGTTCAGG+TGG 0.543751 5.2:+76892822 None:intergenic
GATCTACATGAATCTTCCTA+AGG 0.548247 5.2:-76892163 MS.gene48027:CDS
AGAAACAAGAGCCTCAGCTA+AGG 0.548714 5.2:+76893440 None:intergenic
TTGTTGAAAAGCCATTATGT+TGG 0.550086 5.2:-76892482 MS.gene48027:CDS
GTCGGTCCATCTGCTCCACC+AGG 0.550954 5.2:+76892336 None:intergenic
TGGGTGTCTTGGGGTCAAAA+GGG 0.554761 5.2:+76892902 None:intergenic
GTATAATTGAGCAGGCAACT+TGG 0.556181 5.2:-76893079 MS.gene48027:intron
GGAGGTAGTTGCAGCTACCA+AGG 0.558424 5.2:-76893604 MS.gene48027:CDS
AGTGAAGGATGGATGGAAGG+TGG 0.560310 5.2:+76892882 None:intergenic
CCAAGGCAATGCAAGAACAT+AGG 0.562331 5.2:-76893587 MS.gene48027:CDS
GTGGTGCCAATAGTAATCAT+AGG 0.562421 5.2:+76893495 None:intergenic
TGGAGGAGGTAGATCCGAGA+TGG 0.563260 5.2:+76892842 None:intergenic
AATATGATAAGATCCAACTA+CGG 0.564107 5.2:+76892251 None:intergenic
TAGAGTGAAGGATGGATGGA+AGG 0.564472 5.2:+76892879 None:intergenic
CAGGCAACTTGGATGAAATG+TGG 0.564539 5.2:-76893068 MS.gene48027:CDS
AGGCAACTTGGATGAAATGT+GGG 0.566639 5.2:-76893067 MS.gene48027:CDS
ATTTCCACTATTGGTTGAAG+CGG 0.566759 5.2:+76892641 None:intergenic
CTTGGGACTAATTGATTGGG+TGG 0.567048 5.2:-76892406 MS.gene48027:CDS
AGACTTGTTATTGCTCGAGC+GGG 0.569461 5.2:+76892959 None:intergenic
ACAAAGATTTCCAGAGTCAA+AGG 0.570544 5.2:-76892295 MS.gene48027:CDS
GGAGTTCCTGGTGGAGCAGA+TGG 0.571944 5.2:-76892342 MS.gene48027:CDS
TGAAATGGTCACTAACTCCA+TGG 0.576847 5.2:-76893400 MS.gene48027:CDS
AGCCAGAGGAAACCATTGAA+GGG 0.581302 5.2:-76893290 MS.gene48027:CDS
AAAAGGGACCTCTGAAAACA+AGG 0.581419 5.2:+76892918 None:intergenic
CCCCACAATAGTGTTATTGG+TGG 0.583667 5.2:+76893476 None:intergenic
TCTTATCATATTCCTTGTGG+TGG 0.583934 5.2:-76892240 MS.gene48027:CDS
AAGAGCATCGACCGGTTCCA+GGG 0.585377 5.2:-76892383 MS.gene48027:CDS
TTTCTTTAGACCAATCTCAT+GGG 0.587261 5.2:-76892106 MS.gene48027:CDS
ATGCCTAGTCCTAAAAGGAA+TGG 0.590231 5.2:-76892195 MS.gene48027:CDS
ATATGATAAGATCCAACTAC+GGG 0.599022 5.2:+76892252 None:intergenic
TGGCATGCAACTTTGACCAT+CGG 0.600034 5.2:-76893034 MS.gene48027:CDS
AAGACTTGTTATTGCTCGAG+CGG 0.603884 5.2:+76892958 None:intergenic
AAGTAGCTCAGGTTCACCCA+TGG 0.604674 5.2:+76893383 None:intergenic
ACTTTGCAACAACCGTGGTG+TGG 0.609718 5.2:-76893364 MS.gene48027:CDS
TTGGGTGGAAGAGCATCGAC+CGG 0.614426 5.2:-76892391 MS.gene48027:CDS
AGCAGATGGACCGACATTTG+TGG 0.619293 5.2:-76892328 MS.gene48027:CDS
AATAGGCGTCCGCTATCCGA+TGG 0.620621 5.2:+76893018 None:intergenic
TGACATGTACATCTCCATCT+CGG 0.620929 5.2:-76892856 MS.gene48027:CDS
TGTGTTGGCTAGTAACTGCA+TGG 0.621948 5.2:+76892737 None:intergenic
ATGTACATGTCATAGAGTGA+AGG 0.625954 5.2:+76892867 None:intergenic
CAGTGTGAATATTACCAAAG+AGG 0.626621 5.2:-76893625 MS.gene48027:CDS
CAACTTTGACCATCGGATAG+CGG 0.635631 5.2:-76893027 MS.gene48027:CDS
CCAGAAGACACCACAAATGT+CGG 0.636633 5.2:+76892318 None:intergenic
CTTGTTCCAAAGAAAACACA+TGG 0.639769 5.2:-76893264 MS.gene48027:CDS
ATTTATTGCGGAGTTCCTGG+TGG 0.643183 5.2:-76892351 MS.gene48027:CDS
GAACACACCAATACTAACAG+GGG 0.649933 5.2:+76893533 None:intergenic
GGCAACTTGGATGAAATGTG+GGG 0.657336 5.2:-76893066 MS.gene48027:CDS
AAAATGGGGATTGTGGTCGA+CGG 0.658600 5.2:-76892609 MS.gene48027:CDS
GGAAGAATGAGATTAAGCAA+TGG 0.658733 5.2:-76892588 MS.gene48027:CDS
TGTGAATATTACCAAAGAGG+AGG 0.660805 5.2:-76893622 MS.gene48027:CDS
GCAACTTGGATGAAATGTGG+GGG 0.664532 5.2:-76893065 MS.gene48027:CDS
AGAACACACCAATACTAACA+GGG 0.664943 5.2:+76893532 None:intergenic
TCTCAATTTGCATAAGCCAG+AGG 0.668470 5.2:-76893304 MS.gene48027:CDS
GGGCTGGCAAGGATTTATTG+CGG 0.669488 5.2:-76892363 MS.gene48027:CDS
GAAATGGTCACTAACTCCAT+GGG 0.670922 5.2:-76893399 MS.gene48027:CDS
ACATGTCATAGAGTGAAGGA+TGG 0.670976 5.2:+76892871 None:intergenic
TAGCCAGCATCACCACCACA+AGG 0.673907 5.2:+76892228 None:intergenic
GAGATTGGTCTAAAGAAATG+GGG 0.682938 5.2:+76892111 None:intergenic
GGAATACTTGCACTTCATGG+AGG 0.690260 5.2:-76892142 MS.gene48027:CDS
CCACCAATAACACTATTGTG+GGG 0.693360 5.2:-76893476 MS.gene48027:CDS
AACACACCAATACTAACAGG+GGG 0.696942 5.2:+76893534 None:intergenic
GAGCTACTTTGCAACAACCG+TGG 0.705966 5.2:-76893369 MS.gene48027:CDS
CAACTTGGATGAAATGTGGG+GGG 0.712514 5.2:-76893064 MS.gene48027:CDS
CTTCAACAAAATCCACACCA+CGG 0.748079 5.2:+76893352 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATTATTATCATTATGC+AGG - chr5.2:76892581-76892600 MS.gene48027:CDS 20.0%
!! TCTATTAATTTACAACTGTA+GGG + chr5.2:76893230-76893249 None:intergenic 20.0%
!! TTCTATTAATTTACAACTGT+AGG + chr5.2:76893231-76893250 None:intergenic 20.0%
!!! GTTTGTTTTAATATCTGATT+CGG + chr5.2:76892934-76892953 None:intergenic 20.0%
!!! TTTGTTTTAATATCTGATTC+GGG + chr5.2:76892933-76892952 None:intergenic 20.0%
! AAACATGTTTCTTGTATCAT+GGG - chr5.2:76892746-76892765 MS.gene48027:CDS 25.0%
! AAGAAACATGTTTGTAGAAT+AGG + chr5.2:76892739-76892758 None:intergenic 25.0%
! AATATGATAAGATCCAACTA+CGG + chr5.2:76893489-76893508 None:intergenic 25.0%
! AATTGTATGTATAATTGAGC+AGG - chr5.2:76892650-76892669 MS.gene48027:CDS 25.0%
! ATGAATCCTATGATTACTAT+TGG - chr5.2:76892236-76892255 MS.gene48027:CDS 25.0%
!! AGTTTATGAGTTCGTATTTT+GGG - chr5.2:76893189-76893208 MS.gene48027:intron 25.0%
!!! ACAAAAACATTTTGATCTCA+AGG - chr5.2:76892541-76892560 MS.gene48027:CDS 25.0%
!!! CAAAAACATTTTGATCTCAA+GGG - chr5.2:76892542-76892561 MS.gene48027:CDS 25.0%
ATATGATAAGATCCAACTAC+GGG + chr5.2:76893488-76893507 None:intergenic 30.0%
ATTTCTTGGGACTAATTGAT+TGG - chr5.2:76893327-76893346 MS.gene48027:CDS 30.0%
ATTTCTTTAGACCAATCTCA+TGG - chr5.2:76893630-76893649 MS.gene48027:CDS 30.0%
CAAACATGTTTCTTGTATCA+TGG - chr5.2:76892745-76892764 MS.gene48027:CDS 30.0%
GAAATGTTGTTGCAAAAATG+GGG - chr5.2:76893114-76893133 MS.gene48027:intron 30.0%
GGAAATGTTGTTGCAAAAAT+GGG - chr5.2:76893113-76893132 MS.gene48027:intron 30.0%
TGGAAATGTTGTTGCAAAAA+TGG - chr5.2:76893112-76893131 MS.gene48027:intron 30.0%
TGTTGAAAAGCCATTATGTT+GGG - chr5.2:76893256-76893275 MS.gene48027:CDS 30.0%
TTGTTGAAAAGCCATTATGT+TGG - chr5.2:76893255-76893274 MS.gene48027:CDS 30.0%
TTTCTTGGGACTAATTGATT+GGG - chr5.2:76893328-76893347 MS.gene48027:CDS 30.0%
TTTCTTTAGACCAATCTCAT+GGG - chr5.2:76893631-76893650 MS.gene48027:CDS 30.0%
! AATCATTTTCTGCTTTCTTG+TGG - chr5.2:76893057-76893076 MS.gene48027:CDS 30.0%
! GAGTTTATGAGTTCGTATTT+TGG - chr5.2:76893188-76893207 MS.gene48027:intron 30.0%
!! ATGAGATTGGTCTAAAGAAA+TGG + chr5.2:76893631-76893650 None:intergenic 30.0%
!! TGAGATTGGTCTAAAGAAAT+GGG + chr5.2:76893630-76893649 None:intergenic 30.0%
AAGAACACACCAATACTAAC+AGG + chr5.2:76892209-76892228 None:intergenic 35.0%
ACAAAGATTTCCAGAGTCAA+AGG - chr5.2:76893442-76893461 MS.gene48027:CDS 35.0%
ACCACCAATAACACTATTGT+GGG - chr5.2:76892260-76892279 MS.gene48027:CDS 35.0%
AGAACACACCAATACTAACA+GGG + chr5.2:76892208-76892227 None:intergenic 35.0%
ATGAAGTGCAAGTATTCCTT+AGG + chr5.2:76893593-76893612 None:intergenic 35.0%
ATGTACATGTCATAGAGTGA+AGG + chr5.2:76892873-76892892 None:intergenic 35.0%
CAGTGTGAATATTACCAAAG+AGG - chr5.2:76892112-76892131 MS.gene48027:CDS 35.0%
CTAGCCAACACAAATAATAG+TGG - chr5.2:76893011-76893030 MS.gene48027:CDS 35.0%
CTTGTTCCAAAGAAAACACA+TGG - chr5.2:76892473-76892492 MS.gene48027:CDS 35.0%
GACACGAAAGAACATTTCTT+GGG - chr5.2:76893314-76893333 MS.gene48027:CDS 35.0%
GATCTACATGAATCTTCCTA+AGG - chr5.2:76893574-76893593 MS.gene48027:CDS 35.0%
GCTTCCACTATTATTTGTGT+TGG + chr5.2:76893018-76893037 None:intergenic 35.0%
GGAAGAATGAGATTAAGCAA+TGG - chr5.2:76893149-76893168 MS.gene48027:intron 35.0%
GGATCTTATCATATTCCTTG+TGG - chr5.2:76893494-76893513 MS.gene48027:CDS 35.0%
GTTTCTTATTACGTCTTTGC+TGG - chr5.2:76892314-76892333 MS.gene48027:CDS 35.0%
TAAGGAATACTTGCACTTCA+TGG - chr5.2:76893592-76893611 MS.gene48027:CDS 35.0%
TCTTATCATATTCCTTGTGG+TGG - chr5.2:76893497-76893516 MS.gene48027:CDS 35.0%
TGCAACAACATTTCCACTAT+TGG + chr5.2:76893108-76893127 None:intergenic 35.0%
TGTGAATATTACCAAAGAGG+AGG - chr5.2:76892115-76892134 MS.gene48027:CDS 35.0%
TTTGAAAAACTCCTTAGCTG+AGG - chr5.2:76892286-76892305 MS.gene48027:CDS 35.0%
! AACCAATCACCATTCCTTTT+AGG + chr5.2:76893554-76893573 None:intergenic 35.0%
! AGTCAAAGGTAGATTTTGGA+TGG - chr5.2:76893456-76893475 MS.gene48027:CDS 35.0%
! ATTTCCACTATTGGTTGAAG+CGG + chr5.2:76893099-76893118 None:intergenic 35.0%
! GTCAAAGGTAGATTTTGGAT+GGG - chr5.2:76893457-76893476 MS.gene48027:CDS 35.0%
! TCAAAGGTAGATTTTGGATG+GGG - chr5.2:76893458-76893477 MS.gene48027:CDS 35.0%
!! GAGATTGGTCTAAAGAAATG+GGG + chr5.2:76893629-76893648 None:intergenic 35.0%
!! TTAATATCTGATTCGGGCTT+GGG + chr5.2:76892927-76892946 None:intergenic 35.0%
!! TTTAATATCTGATTCGGGCT+TGG + chr5.2:76892928-76892947 None:intergenic 35.0%
!!! AGAGCGAGTTCATGATTTTT+TGG - chr5.2:76893283-76893302 MS.gene48027:CDS 35.0%
AACACACCAATACTAACAGG+GGG + chr5.2:76892206-76892225 None:intergenic 40.0%
ACACCCCACAATAGTGTTAT+TGG + chr5.2:76892267-76892286 None:intergenic 40.0%
ACATGTCATAGAGTGAAGGA+TGG + chr5.2:76892869-76892888 None:intergenic 40.0%
AGAAGGTAATCCCATGAGAT+TGG + chr5.2:76893644-76893663 None:intergenic 40.0%
AGGCAACTTGGATGAAATGT+GGG - chr5.2:76892670-76892689 MS.gene48027:CDS 40.0%
AGTACATGCGACTTATTAGC+TGG + chr5.2:76892956-76892975 None:intergenic 40.0%
ATGCCTAGTCCTAAAAGGAA+TGG - chr5.2:76893542-76893561 MS.gene48027:CDS 40.0%
CACCACCAATAACACTATTG+TGG - chr5.2:76892259-76892278 MS.gene48027:CDS 40.0%
CCAAAATCTACCTTTGACTC+TGG + chr5.2:76893455-76893474 None:intergenic 40.0%
CCACCAATAACACTATTGTG+GGG - chr5.2:76892261-76892280 MS.gene48027:CDS 40.0%
CTTCAACAAAATCCACACCA+CGG + chr5.2:76892388-76892407 None:intergenic 40.0%
GAAATGGTCACTAACTCCAT+GGG - chr5.2:76892338-76892357 MS.gene48027:CDS 40.0%
GAACACACCAATACTAACAG+GGG + chr5.2:76892207-76892226 None:intergenic 40.0%
GGACACGAAAGAACATTTCT+TGG - chr5.2:76893313-76893332 MS.gene48027:CDS 40.0%
GTATAATTGAGCAGGCAACT+TGG - chr5.2:76892658-76892677 MS.gene48027:CDS 40.0%
GTCCTAAAAGGAATGGTGAT+TGG - chr5.2:76893549-76893568 MS.gene48027:CDS 40.0%
TCTCAATTTGCATAAGCCAG+AGG - chr5.2:76892433-76892452 MS.gene48027:CDS 40.0%
TGAAATGGTCACTAACTCCA+TGG - chr5.2:76892337-76892356 MS.gene48027:CDS 40.0%
TGACATGTACATCTCCATCT+CGG - chr5.2:76892881-76892900 MS.gene48027:CDS 40.0%
TGTTGCAAAAATGGGGATTG+TGG - chr5.2:76893121-76893140 MS.gene48027:intron 40.0%
TTACGTCTTTGCTGGTGAAA+TGG - chr5.2:76892322-76892341 MS.gene48027:CDS 40.0%
TTCTGCTTTCTTGTGGAAGA+TGG - chr5.2:76893064-76893083 MS.gene48027:CDS 40.0%
! AAAAGGGACCTCTGAAAACA+AGG + chr5.2:76892822-76892841 None:intergenic 40.0%
! AGCACACCATGTGTTTTCTT+TGG + chr5.2:76892482-76892501 None:intergenic 40.0%
! CAAAGGTAGATTTTGGATGG+GGG - chr5.2:76893459-76893478 MS.gene48027:CDS 40.0%
! CCAGAGTCAAAGGTAGATTT+TGG - chr5.2:76893452-76893471 MS.gene48027:CDS 40.0%
! GTGGTGCCAATAGTAATCAT+AGG + chr5.2:76892245-76892264 None:intergenic 40.0%
!! AAGACTTGTTATTGCTCGAG+CGG + chr5.2:76892782-76892801 None:intergenic 40.0%
!!! CAACACAACCTTGTTTTCAG+AGG - chr5.2:76892811-76892830 MS.gene48027:CDS 40.0%
!!! GCGAGTTCATGATTTTTTGG+AGG - chr5.2:76893286-76893305 MS.gene48027:CDS 40.0%
!!! TCACCATTCCTTTTAGGACT+AGG + chr5.2:76893548-76893567 None:intergenic 40.0%
!!! TCATGATTTTTTGGAGGCTG+CGG - chr5.2:76893292-76893311 MS.gene48027:CDS 40.0%
!!! TTCCTTTTAGGACTAGGCAT+TGG + chr5.2:76893542-76893561 None:intergenic 40.0%
AAAATGGGGATTGTGGTCGA+CGG - chr5.2:76893128-76893147 MS.gene48027:intron 45.0%
AAGCCAGAGGAAACCATTGA+AGG - chr5.2:76892446-76892465 MS.gene48027:CDS 45.0%
AGAAACAAGAGCCTCAGCTA+AGG + chr5.2:76892300-76892319 None:intergenic 45.0%
AGCCAGAGGAAACCATTGAA+GGG - chr5.2:76892447-76892466 MS.gene48027:CDS 45.0%
AGGATTTATTGCGGAGTTCC+TGG - chr5.2:76893383-76893402 MS.gene48027:CDS 45.0%
ATTTATTGCGGAGTTCCTGG+TGG - chr5.2:76893386-76893405 MS.gene48027:CDS 45.0%
CAACTTGGATGAAATGTGGG+GGG - chr5.2:76892673-76892692 MS.gene48027:CDS 45.0%
CAACTTTGACCATCGGATAG+CGG - chr5.2:76892710-76892729 MS.gene48027:CDS 45.0%
CAGGCAACTTGGATGAAATG+TGG - chr5.2:76892669-76892688 MS.gene48027:CDS 45.0%
CCAAGGCAATGCAAGAACAT+AGG - chr5.2:76892150-76892169 MS.gene48027:CDS 45.0%
CCAGAAGACACCACAAATGT+CGG + chr5.2:76893422-76893441 None:intergenic 45.0%
CCTATGTTCTTGCATTGCCT+TGG + chr5.2:76892153-76892172 None:intergenic 45.0%
CGCTCTGTAACCCAACATAA+TGG + chr5.2:76893269-76893288 None:intergenic 45.0%
CTACTTCCCCCTGTTAGTAT+TGG - chr5.2:76892197-76892216 MS.gene48027:CDS 45.0%
CTTGGGACTAATTGATTGGG+TGG - chr5.2:76893331-76893350 MS.gene48027:CDS 45.0%
GCAACTTGGATGAAATGTGG+GGG - chr5.2:76892672-76892691 MS.gene48027:CDS 45.0%
GGAATACTTGCACTTCATGG+AGG - chr5.2:76893595-76893614 MS.gene48027:CDS 45.0%
GGCAACTTGGATGAAATGTG+GGG - chr5.2:76892671-76892690 MS.gene48027:CDS 45.0%
GTCATAGAGTGAAGGATGGA+TGG + chr5.2:76892865-76892884 None:intergenic 45.0%
TAGAGTGAAGGATGGATGGA+AGG + chr5.2:76892861-76892880 None:intergenic 45.0%
TGCCAATGCCTAGTCCTAAA+AGG - chr5.2:76893537-76893556 MS.gene48027:CDS 45.0%
TGGAACAAGCTTCCCTTCAA+TGG + chr5.2:76892462-76892481 None:intergenic 45.0%
TGGCATGCAACTTTGACCAT+CGG - chr5.2:76892703-76892722 MS.gene48027:CDS 45.0%
TTCCCTTCAATGGTTTCCTC+TGG + chr5.2:76892452-76892471 None:intergenic 45.0%
! CCCCACAATAGTGTTATTGG+TGG + chr5.2:76892264-76892283 None:intergenic 45.0%
!! AGACTTGTTATTGCTCGAGC+GGG + chr5.2:76892781-76892800 None:intergenic 45.0%
!! TGTGTTGGCTAGTAACTGCA+TGG + chr5.2:76893003-76893022 None:intergenic 45.0%
AAGTAGCTCAGGTTCACCCA+TGG + chr5.2:76892357-76892376 None:intergenic 50.0%
ACTTTGCAACAACCGTGGTG+TGG - chr5.2:76892373-76892392 MS.gene48027:CDS 50.0%
AGCAGATGGACCGACATTTG+TGG - chr5.2:76893409-76893428 MS.gene48027:CDS 50.0%
AGCTGCAACTACCTCCTCTT+TGG + chr5.2:76892129-76892148 None:intergenic 50.0%
AGTGAAGGATGGATGGAAGG+TGG + chr5.2:76892858-76892877 None:intergenic 50.0%
CAATAAATCCTTGCCAGCCC+TGG + chr5.2:76893374-76893393 None:intergenic 50.0%
CCGACATTTGTGGTGTCTTC+TGG - chr5.2:76893419-76893438 MS.gene48027:CDS 50.0%
CGGTTGTTGCAAAGTAGCTC+AGG + chr5.2:76892368-76892387 None:intergenic 50.0%
GTGAAGGATGGATGGAAGGT+GGG + chr5.2:76892857-76892876 None:intergenic 50.0%
TCAGCCGCTTCAACCAATAG+TGG - chr5.2:76893092-76893111 MS.gene48027:intron 50.0%
TGGGTGTCTTGGGGTCAAAA+GGG + chr5.2:76892838-76892857 None:intergenic 50.0%
! ATTCCTTGTGGTGGTGATGC+TGG - chr5.2:76893506-76893525 MS.gene48027:CDS 50.0%
! GAAATGTGGGGGGTTAGTAG+TGG - chr5.2:76892683-76892702 MS.gene48027:CDS 50.0%
! GAGCTACTTTGCAACAACCG+TGG - chr5.2:76892368-76892387 MS.gene48027:CDS 50.0%
! GGGCTGGCAAGGATTTATTG+CGG - chr5.2:76893374-76893393 MS.gene48027:CDS 50.0%
!! ACATGGTGTGCTTGCTGTTC+AGG - chr5.2:76892490-76892509 MS.gene48027:CDS 50.0%
AATAGGCGTCCGCTATCCGA+TGG + chr5.2:76892722-76892741 None:intergenic 55.0%
GATGGAAGGTGGGTGTCTTG+GGG + chr5.2:76892847-76892866 None:intergenic 55.0%
GGAGGTAGTTGCAGCTACCA+AGG - chr5.2:76892133-76892152 MS.gene48027:CDS 55.0%
GGATGGAAGGTGGGTGTCTT+GGG + chr5.2:76892848-76892867 None:intergenic 55.0%
GTGGGTGTCTTGGGGTCAAA+AGG + chr5.2:76892839-76892858 None:intergenic 55.0%
TAGCCAGCATCACCACCACA+AGG + chr5.2:76893512-76893531 None:intergenic 55.0%
TGGAGGAGGTAGATCCGAGA+TGG + chr5.2:76892898-76892917 None:intergenic 55.0%
TGGATGGAAGGTGGGTGTCT+TGG + chr5.2:76892849-76892868 None:intergenic 55.0%
!! AAGAGCATCGACCGGTTCCA+GGG - chr5.2:76893354-76893373 MS.gene48027:CDS 55.0%
!! ATTGCTCGAGCGGGCTATCT+CGG + chr5.2:76892772-76892791 None:intergenic 55.0%
!! TCTGATTCGGGCTTGGGTTC+AGG + chr5.2:76892921-76892940 None:intergenic 55.0%
!! TTGGGTGGAAGAGCATCGAC+CGG - chr5.2:76893346-76893365 MS.gene48027:CDS 55.0%
ATCCTTGCCAGCCCTGGAAC+CGG + chr5.2:76893368-76893387 None:intergenic 60.0%
GATTCGGGCTTGGGTTCAGG+TGG + chr5.2:76892918-76892937 None:intergenic 60.0%
GGAGTTCCTGGTGGAGCAGA+TGG - chr5.2:76893395-76893414 MS.gene48027:CDS 60.0%
GGATGGGGGAAACCCGTAGT+TGG - chr5.2:76893473-76893492 MS.gene48027:CDS 60.0%
!! GAAGAGCATCGACCGGTTCC+AGG - chr5.2:76893353-76893372 MS.gene48027:CDS 60.0%
GGCTTGGGTTCAGGTGGAGG+AGG + chr5.2:76892912-76892931 None:intergenic 65.0%
GTCGGTCCATCTGCTCCACC+AGG + chr5.2:76893404-76893423 None:intergenic 65.0%
TCGGGCTTGGGTTCAGGTGG+AGG + chr5.2:76892915-76892934 None:intergenic 65.0%
! GACCGGTTCCAGGGCTGGCA+AGG - chr5.2:76893363-76893382 MS.gene48027:CDS 70.0%
! GCATCGACCGGTTCCAGGGC+TGG - chr5.2:76893358-76893377 MS.gene48027:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 76892089 76893670 76892089 ID=MS.gene48027
chr5.2 mRNA 76892089 76893670 76892089 ID=MS.gene48027.t1;Parent=MS.gene48027
chr5.2 exon 76893248 76893670 76893248 ID=MS.gene48027.t1.exon1;Parent=MS.gene48027.t1
chr5.2 CDS 76893248 76893670 76893248 ID=cds.MS.gene48027.t1;Parent=MS.gene48027.t1
chr5.2 exon 76892089 76893087 76892089 ID=MS.gene48027.t1.exon2;Parent=MS.gene48027.t1
chr5.2 CDS 76892089 76893087 76892089 ID=cds.MS.gene48027.t1;Parent=MS.gene48027.t1
Gene Sequence

>MS.gene48027

ATGAGTATTGGAAAAGTAGAATTCAGTGTGAATATTACCAAAGAGGAGGTAGTTGCAGCTACCAAGGCAATGCAAGAACATAGGCTAGCACTATCAAATCTTGATTTACTACTTCCCCCTGTTAGTATTGGTGTGTTCTTTTGCTACATGAATCCTATGATTACTATTGGCACCACCAATAACACTATTGTGGGGTGTTTGAAAAACTCCTTAGCTGAGGCTCTTGTTTCTTATTACGTCTTTGCTGGTGAAATGGTCACTAACTCCATGGGTGAACCTGAGCTACTTTGCAACAACCGTGGTGTGGATTTTGTTGAAGCTGTTGTTGATGTTGAGTTACAATCTCTCAATTTGCATAAGCCAGAGGAAACCATTGAAGGGAAGCTTGTTCCAAAGAAAACACATGGTGTGCTTGCTGTTCAGGTGAAAGTTTTTAATATTTGTTCATATTTACAAAAACATTTTGATCTCAAGGGAGTATTAGAATAGTGAAAGAATTATTATCATTATGCAGGTTTTTAGTTAATGATTAGTAGTAAGAAGCGTAGTAAGAAGTGTTTCAATTGTATGTATAATTGAGCAGGCAACTTGGATGAAATGTGGGGGGTTAGTAGTGGCATGCAACTTTGACCATCGGATAGCGGACGCCTATTCTACAAACATGTTTCTTGTATCATGGGCCGAGATAGCCCGCTCGAGCAATAACAAGTCTTTGATCTCAGCAACACAACCTTGTTTTCAGAGGTCCCTTTTGACCCCAAGACACCCACCTTCCATCCATCCTTCACTCTATGACATGTACATCTCCATCTCGGATCTACCTCCTCCACCTGAACCCAAGCCCGAATCAGATATTAAAACAAACCAGCTAATAAGTCGCATGTACTATGTAACAAGCGAAGAACTCAACACCATGCAGTTACTAGCCAACACAAATAATAGTGGAAGCATTAAGCGCTCAAAACTTGAATCATTTTCTGCTTTCTTGTGGAAGATGGTTGCATCAGCCGCTTCAACCAATAGTGGAAATGTTGTTGCAAAAATGGGGATTGTGGTCGACGGAAGAATGAGATTAAGCAATGGTGACAAAAACAAAGAAGAGTTTATGAGTTCGTATTTTGGGAATGTGCTTTCAACTCCCTACAGTTGTAAATTAATAGAAGAACTTGTTGAAAAGCCATTATGTTGGGTTACAGAGCGAGTTCATGATTTTTTGGAGGCTGCGGACACGAAAGAACATTTCTTGGGACTAATTGATTGGGTGGAAGAGCATCGACCGGTTCCAGGGCTGGCAAGGATTTATTGCGGAGTTCCTGGTGGAGCAGATGGACCGACATTTGTGGTGTCTTCTGGACAAAGATTTCCAGAGTCAAAGGTAGATTTTGGATGGGGGAAACCCGTAGTTGGATCTTATCATATTCCTTGTGGTGGTGATGCTGGCTATGTTATGCCAATGCCTAGTCCTAAAAGGAATGGTGATTGGTTGATCTACATGAATCTTCCTAAGGAATACTTGCACTTCATGGAGGCTCAAGCTCCCCATTTCTTTAGACCAATCTCATGGGATTACCTTCTCAATTGA
Protein sequence

>MS.gene48027.t1

MSIGKVEFSVNITKEEVVAATKAMQEHRLALSNLDLLLPPVSIGVFFCYMNPMITIGTTNNTIVGCLKNSLAEALVSYYVFAGEMVTNSMGEPELLCNNRGVDFVEAVVDVELQSLNLHKPEETIEGKLVPKKTHGVLAVQATWMKCGGLVVACNFDHRIADAYSTNMFLVSWAEIARSSNNKSLISATQPCFQRSLLTPRHPPSIHPSLYDMYISISDLPPPPEPKPESDIKTNQLISRMYYVTSEELNTMQLLANTNNSGSIKRSKLESFSAFLWKMVASAASTNSGNVVAKMGIVVDGRMRLSNGDKNKEEFMSSYFGNVLSTPYSCKLIEELVEKPLCWVTERVHDFLEAADTKEHFLGLIDWVEEHRPVPGLARIYCGVPGGADGPTFVVSSGQRFPESKVDFGWGKPVVGSYHIPCGGDAGYVMPMPSPKRNGDWLIYMNLPKEYLHFMEAQAPHFFRPISWDYLLN