AlfalfaGEDB | MS.gene47721

M. sativa cultivar XinJiangDaYe / MS.gene47721

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47721.t1	XP_003617368.1	93.4	473	28	2	1	472	1	471	2.60E-265	924.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47721.t1	Q9FI78	25.1	459	301	16	8	455	1	427	8.6e-31	136.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47721.t1	G7K3A2	93.4	473	28	2	1	472	1	471	1.9e-265	924.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049549	MS.gene47721	0.844836	5.91E-59	-1.69E-46
MS.gene051127	MS.gene47721	0.816707	4.81E-52	-1.69E-46
MS.gene052211	MS.gene47721	0.858282	9.17E-63	-1.69E-46
MS.gene052212	MS.gene47721	0.825802	3.84E-54	-1.69E-46
MS.gene05240	MS.gene47721	0.826659	2.40E-54	-1.69E-46
MS.gene053406	MS.gene47721	0.820699	5.97E-53	-1.69E-46
MS.gene053887	MS.gene47721	0.803323	3.68E-49	-1.69E-46
MS.gene054146	MS.gene47721	0.848827	4.78E-60	-1.69E-46
MS.gene05483	MS.gene47721	0.808585	2.88E-50	-1.69E-46
MS.gene055240	MS.gene47721	0.814154	1.78E-51	-1.69E-46
MS.gene056218	MS.gene47721	0.821667	3.57E-53	-1.69E-46
MS.gene056224	MS.gene47721	0.80549	1.30E-49	-1.69E-46
MS.gene056715	MS.gene47721	0.804286	2.32E-49	-1.69E-46
MS.gene056748	MS.gene47721	0.812122	4.97E-51	-1.69E-46
MS.gene056920	MS.gene47721	0.805057	1.60E-49	-1.69E-46
MS.gene057261	MS.gene47721	0.802627	5.13E-49	-1.69E-46
MS.gene057550	MS.gene47721	0.804311	2.30E-49	-1.69E-46
MS.gene058603	MS.gene47721	0.815122	1.09E-51	-1.69E-46
MS.gene059265	MS.gene47721	0.828699	7.77E-55	-1.69E-46
MS.gene059955	MS.gene47721	0.810305	1.23E-50	-1.69E-46
MS.gene060087	MS.gene47721	0.815391	9.47E-52	-1.69E-46
MS.gene061092	MS.gene47721	0.806066	9.85E-50	-1.69E-46
MS.gene061094	MS.gene47721	0.811614	6.41E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene017058	MS.gene47721	PPI
MS.gene47721	MS.gene012959	PPI
MS.gene028134	MS.gene47721	PPI
MS.gene47721	MS.gene47719	PPI
MS.gene47721	MS.gene82145	PPI
MS.gene22199	MS.gene47721	PPI
MS.gene95242	MS.gene47721	PPI
MS.gene32936	MS.gene47721	PPI
MS.gene72785	MS.gene47721	PPI
MS.gene47721	MS.gene000507	PPI
MS.gene017054	MS.gene47721	PPI
MS.gene48027	MS.gene47721	PPI
MS.gene47721	MS.gene38702	PPI
MS.gene83461	MS.gene47721	PPI
MS.gene32938	MS.gene47721	PPI
MS.gene48021	MS.gene47721	PPI
MS.gene38362	MS.gene47721	PPI
MS.gene47721	MS.gene38703	PPI
MS.gene017053	MS.gene47721	PPI
MS.gene47716	MS.gene47721	PPI
MS.gene47721	MS.gene012961	PPI
MS.gene47721	MS.gene038274	PPI
MS.gene38363	MS.gene47721	PPI
MS.gene38706	MS.gene47721	PPI
MS.gene32934	MS.gene47721	PPI
MS.gene48023	MS.gene47721	PPI
MS.gene47721	MS.gene38359	PPI
MS.gene012960	MS.gene47721	PPI
MS.gene38704	MS.gene47721	PPI
MS.gene47721	MS.gene039597	PPI
MS.gene069321	MS.gene47721	PPI
MS.gene47721	MS.gene32933	PPI
MS.gene38361	MS.gene47721	PPI
MS.gene38701	MS.gene47721	PPI
MS.gene046246	MS.gene47721	PPI
MS.gene38700	MS.gene47721	PPI
MS.gene47721	MS.gene69496	PPI
MS.gene38365	MS.gene47721	PPI
MS.gene012965	MS.gene47721	PPI
MS.gene012957	MS.gene47721	PPI
MS.gene47721	MS.gene38699	PPI
MS.gene019178	MS.gene47721	PPI
MS.gene47721	MS.gene038271	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47721.t1	MTR_5g090830	93.446	473	28	2	1	472	1	471	0.0	920
MS.gene47721.t1	MTR_5g090810	92.178	473	34	2	1	472	1	471	0.0	909
MS.gene47721.t1	MTR_5g090880	80.127	473	93	1	1	472	1	473	0.0	781
MS.gene47721.t1	MTR_3g450710	68.085	470	129	7	10	470	11	468	0.0	629
MS.gene47721.t1	MTR_3g450690	64.894	470	148	5	10	470	11	472	0.0	612
MS.gene47721.t1	MTR_5g026320	36.867	415	234	12	29	440	39	428	1.51e-72	236
MS.gene47721.t1	MTR_8g096530	27.706	462	285	18	7	458	4	426	5.73e-34	133
MS.gene47721.t1	MTR_8g075610	25.747	435	276	15	32	455	16	414	6.96e-33	129
MS.gene47721.t1	MTR_2g064495	25.486	463	289	18	10	455	3	426	1.22e-31	126
MS.gene47721.t1	MTR_2g105330	25.486	463	289	18	10	455	3	426	4.54e-31	124
MS.gene47721.t1	MTR_4g007540	23.913	414	278	12	10	414	3	388	4.23e-29	119
MS.gene47721.t1	MTR_3g098980	26.854	391	242	14	31	413	35	389	3.89e-28	116
MS.gene47721.t1	MTR_4g097090	25.376	465	298	15	23	465	19	456	4.78e-28	116
MS.gene47721.t1	MTR_3g049150	25.740	439	273	18	31	446	23	431	9.89e-28	115
MS.gene47721.t1	MTR_4g127650	23.861	461	309	12	23	465	20	456	3.50e-27	114
MS.gene47721.t1	MTR_7g086000	29.032	372	216	15	66	412	60	408	7.50e-27	113
MS.gene47721.t1	MTR_5g076700	25.253	396	249	13	66	447	65	427	2.68e-26	111
MS.gene47721.t1	MTR_1g040215	26.617	402	232	14	63	436	61	427	3.18e-26	111
MS.gene47721.t1	MTR_7g114470	25.592	422	264	16	8	415	9	394	6.52e-26	110
MS.gene47721.t1	MTR_7g027175	25.653	421	267	15	4	414	9	393	1.62e-25	108
MS.gene47721.t1	MTR_7g114520	25.926	459	281	18	8	446	9	428	1.92e-25	108
MS.gene47721.t1	MTR_7g114560	23.929	397	258	11	63	447	62	426	7.06e-25	107
MS.gene47721.t1	MTR_1g081660	26.273	373	229	15	63	424	70	407	3.08e-24	105
MS.gene47721.t1	MTR_7g114550	24.301	465	295	16	1	447	1	426	4.84e-24	104
MS.gene47721.t1	MTR_1g040175	25.310	403	236	15	63	436	61	427	4.94e-24	104
MS.gene47721.t1	MTR_7g114490	24.029	412	261	13	63	458	62	437	5.94e-23	102
MS.gene47721.t1	MTR_1g053315	24.816	407	252	15	29	415	40	412	1.68e-22	100
MS.gene47721.t1	MTR_3g026950	24.312	436	287	13	31	446	23	435	1.12e-21	97.8
MS.gene47721.t1	MTR_7g085930	27.848	395	233	19	66	429	59	432	1.48e-21	97.4
MS.gene47721.t1	MTR_7g085990	25.916	382	227	17	66	414	55	413	3.34e-21	96.3
MS.gene47721.t1	MTR_2g015430	24.129	402	258	17	24	413	18	384	5.71e-19	89.4
MS.gene47721.t1	MTR_4g055170	24.176	455	297	18	17	451	19	445	3.82e-18	87.0
MS.gene47721.t1	MTR_3g101900	22.651	415	257	11	27	413	19	397	6.20e-18	86.3
MS.gene47721.t1	MTR_1g040195	24.523	367	216	14	96	436	71	402	1.49e-17	85.1
MS.gene47721.t1	MTR_3g086020	23.908	435	254	20	6	426	1	372	1.80e-17	84.7
MS.gene47721.t1	MTR_7g084940	25.694	432	249	18	6	414	12	394	2.57e-17	84.3
MS.gene47721.t1	MTR_5g084870	22.619	420	269	16	8	414	1	377	2.43e-16	81.3
MS.gene47721.t1	MTR_3g101960	23.292	322	198	12	49	348	48	342	3.66e-16	80.9
MS.gene47721.t1	MTR_3g101930	23.450	371	232	11	61	411	65	403	6.37e-16	80.1
MS.gene47721.t1	MTR_7g084910	25.435	460	256	19	7	434	14	418	1.01e-15	79.3
MS.gene47721.t1	MTR_3g070170	23.454	388	235	14	60	413	60	419	1.76e-15	79.0
MS.gene47721.t1	MTR_8g079950	22.558	430	263	14	8	414	1	383	3.39e-15	78.2
MS.gene47721.t1	MTR_3g465990	25.240	313	210	9	66	370	68	364	3.89e-15	77.8
MS.gene47721.t1	MTR_3g112180	22.143	420	250	15	18	411	20	388	5.67e-15	77.4
MS.gene47721.t1	MTR_7g114510	24.727	275	175	9	142	415	9	252	2.48e-14	74.3
MS.gene47721.t1	MTR_5g084850	22.803	421	266	14	8	414	1	376	2.38e-13	72.0
MS.gene47721.t1	MTR_3g088420	24.000	400	236	17	36	413	32	385	4.36e-13	71.2
MS.gene47721.t1	MTR_3g465980	24.299	321	203	9	66	370	68	364	6.05e-13	70.9
MS.gene47721.t1	MTR_4g094828	23.573	403	243	16	36	413	37	399	1.62e-12	69.7
MS.gene47721.t1	MTR_8g041770	23.971	413	249	15	23	413	22	391	2.96e-12	68.9
MS.gene47721.t1	MTR_7g110610	29.167	144	91	3	66	202	55	194	3.12e-12	68.6
MS.gene47721.t1	MTR_4g022670	23.864	440	272	16	49	466	43	441	6.00e-11	64.7
MS.gene47721.t1	MTR_3g102020	21.967	305	196	12	61	348	64	343	7.06e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47721.t1	AT1G32910	58.351	473	162	12	8	470	11	458	1.43e-179	511
MS.gene47721.t1	AT1G78990	56.838	468	172	10	8	470	11	453	9.18e-179	508
MS.gene47721.t1	AT5G16410	51.368	475	190	14	8	470	34	479	3.94e-159	459
MS.gene47721.t1	AT1G31490	36.878	442	237	12	8	440	4	412	6.07e-78	250
MS.gene47721.t1	AT5G48930	25.163	461	298	17	8	455	1	427	1.04e-35	137
MS.gene47721.t1	AT5G41040	25.316	395	248	14	31	414	38	396	1.71e-27	114
MS.gene47721.t1	AT5G41040	25.316	395	248	14	31	414	54	412	2.55e-27	114
MS.gene47721.t1	AT2G19070	24.034	466	310	16	18	465	12	451	7.61e-27	112
MS.gene47721.t1	AT3G03480	25.862	464	269	19	6	436	15	436	6.12e-26	110
MS.gene47721.t1	AT5G57840	26.282	468	280	19	8	451	1	427	6.40e-26	110
MS.gene47721.t1	AT3G48720	27.089	395	239	13	31	414	30	386	7.95e-25	106
MS.gene47721.t1	AT5G17540	25.000	468	264	19	4	439	3	415	1.67e-24	106
MS.gene47721.t1	AT3G50270	27.394	376	218	16	60	413	56	398	7.45e-21	95.1
MS.gene47721.t1	AT1G27620	22.195	401	265	13	66	455	58	422	1.56e-20	94.4
MS.gene47721.t1	AT5G63560	25.672	409	243	15	19	413	20	381	3.29e-20	93.2
MS.gene47721.t1	AT3G50280	25.000	428	261	18	9	413	3	393	5.34e-20	92.8
MS.gene47721.t1	AT1G24430	25.472	424	248	18	8	413	3	376	9.21e-20	92.0
MS.gene47721.t1	AT1G65450	25.926	405	248	16	31	418	40	409	9.58e-19	89.0
MS.gene47721.t1	AT3G30280	25.513	439	265	14	8	431	4	395	1.12e-17	85.5
MS.gene47721.t1	AT1G28680	25.298	419	264	19	12	413	1	387	2.92e-17	84.3
MS.gene47721.t1	AT5G07860	21.583	417	249	15	27	413	30	398	3.10e-17	84.3
MS.gene47721.t1	AT3G26040	22.654	437	250	15	8	413	1	380	5.18e-17	83.6
MS.gene47721.t1	AT5G38130	24.297	391	238	16	60	429	73	426	4.04e-16	80.9
MS.gene47721.t1	AT5G01210	25.393	382	200	15	79	413	75	418	6.32e-15	77.4
MS.gene47721.t1	AT5G07080	23.913	368	238	10	60	413	64	403	7.38e-15	77.0
MS.gene47721.t1	AT5G47950	23.810	420	254	15	8	414	4	370	8.29e-15	76.6
MS.gene47721.t1	AT3G50300	23.784	370	231	15	63	413	56	393	1.21e-14	76.3
MS.gene47721.t1	AT4G15390	23.404	423	270	15	8	414	6	390	2.84e-14	75.1
MS.gene47721.t1	AT4G15390	23.404	423	270	15	8	414	6	390	2.84e-14	75.1
MS.gene47721.t1	AT5G42830	23.352	364	234	13	66	413	64	398	8.48e-14	73.6
MS.gene47721.t1	AT2G25150	22.192	365	242	11	63	413	64	400	1.29e-13	73.2
MS.gene47721.t1	AT1G24420	23.129	441	260	17	6	419	1	389	1.37e-13	73.2
MS.gene47721.t1	AT1G24420	23.129	441	260	17	6	419	1	389	1.37e-13	73.2
MS.gene47721.t1	AT5G67160	24.424	434	254	20	59	472	49	428	4.25e-13	71.6
MS.gene47721.t1	AT3G50290	24.286	280	177	11	136	410	20	269	3.59e-11	64.3

Find 127 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGATTATGAATTCATATTT+TGG	0.139298	5.3:-73043882	MS.gene47721:CDS
TTCTGCTTTCTTGTGGAAAA+TGG	0.174817	5.3:-73044006	MS.gene47721:CDS
CCAGAGTCGAAGGTTGATTT+TGG	0.189640	5.3:-73043618	MS.gene47721:CDS
TAGTATATGCGACTTATTAT+TGG	0.243557	5.3:+73044116	None:intergenic
AGCACACCATGTTTCTTCTT+TGG	0.268793	5.3:+73044736	None:intergenic
GTTTCTTATTATGCCTTTGC+TGG	0.282844	5.3:-73044901	MS.gene47721:CDS
TTAATATCAGAGTCAAATTC+AGG	0.299615	5.3:+73044146	None:intergenic
ACATGGTGTGCTTGCTGTTC+AGG	0.312822	5.3:-73044725	MS.gene47721:intron
GGATGGAAGGTGGGCGTCGT+GGG	0.321410	5.3:+73044225	None:intergenic
AGTATATGCGACTTATTATT+GGG	0.330800	5.3:+73044117	None:intergenic
AAGAACACACCGACATCAAC+AGG	0.337593	5.3:+73045006	None:intergenic
ATCATTGGGCTGAGCCATTT+CGG	0.339987	5.3:+73044301	None:intergenic
TAAGGGACACTTGCACTTCA+TGG	0.343370	5.3:-73043478	MS.gene47721:CDS
GGTGACAGAGGAGCATTTCT+TGG	0.346724	5.3:-73043757	MS.gene47721:CDS
ATTTCTTGGGTTTAATAGAT+TGG	0.349608	5.3:-73043743	MS.gene47721:CDS
GAAGAGCATCGTCCAGTTCC+TGG	0.354253	5.3:-73043717	MS.gene47721:CDS
TGGATGGAAGGTGGGCGTCG+TGG	0.357861	5.3:+73044224	None:intergenic
TGCCAATGCCAAGCCCTAAA+AGG	0.358606	5.3:-73043533	MS.gene47721:CDS
TTTCCTTGGGGAGGTAGTGC+TGG	0.358995	5.3:-73043564	MS.gene47721:CDS
AAGGAACATGTTTGCAGAAT+AGG	0.360809	5.3:+73044334	None:intergenic
TTCCCTTCTACAGTTTCATC+TGG	0.371632	5.3:+73044766	None:intergenic
CGAAAACAAGGTTGCGTTGT+TGG	0.373331	5.3:+73044263	None:intergenic
ATTTCTTTAGACCAGTCTCT+TGG	0.380544	5.3:-73043440	MS.gene47721:CDS
TTTCTTGGGTTTAATAGATT+GGG	0.393187	5.3:-73043742	MS.gene47721:CDS
CCAAAATCAACCTTCGACTC+TGG	0.403209	5.3:+73043618	None:intergenic
TCAGAGTCAAATTCAGGTTC+AGG	0.411350	5.3:+73044152	None:intergenic
CAAACATGTTCCTTGTATCA+TGG	0.413069	5.3:-73044325	MS.gene47721:CDS
CTAATCGCTAAAATGTGTAT+TGG	0.419429	5.3:-73045135	None:intergenic
TTTCTTTAGACCAGTCTCTT+GGG	0.423523	5.3:-73043439	MS.gene47721:CDS
ATCCTTGCCAATCCAGGAAC+TGG	0.426912	5.3:+73043705	None:intergenic
CCTTGTATCATGGGCCGAAA+TGG	0.428090	5.3:-73044315	MS.gene47721:CDS
CCAACATTTGTGGTATCTTC+AGG	0.429306	5.3:-73043651	MS.gene47721:CDS
ATTAAATCAAGGTTTGATAG+TGG	0.431310	5.3:+73045036	None:intergenic
GAAAACAAGGTTGCGTTGTT+GGG	0.433945	5.3:+73044264	None:intergenic
AATCCAGATGAAACTGTAGA+AGG	0.442984	5.3:-73044769	MS.gene47721:CDS
GTGACAGAGGAGCATTTCTT+GGG	0.453426	5.3:-73043756	MS.gene47721:CDS
ATGAAGTGCAAGTGTCCCTT+AGG	0.455932	5.3:+73043480	None:intergenic
TTTCCACAAGAAAGCAGAAA+AGG	0.464789	5.3:+73044010	None:intergenic
CGCTACCAATGCAAGAGCAT+TGG	0.468968	5.3:-73045062	MS.gene47721:CDS
GCTCTACATGCACCTTCCTA+AGG	0.469205	5.3:-73043496	MS.gene47721:CDS
GTCTAAAGAAATGAGGAGCC+TGG	0.472034	5.3:+73043451	None:intergenic
TGGGTGCCAGTAGTAATTAT+AGG	0.473421	5.3:+73044970	None:intergenic
TATGACCAACGAGGAAATAG+TGG	0.494458	5.3:-73045091	MS.gene47721:CDS
AGCTGCCACTATTTCCTCGT+TGG	0.494647	5.3:+73045086	None:intergenic
CAATAAATCCTTGCCAATCC+AGG	0.495236	5.3:+73043699	None:intergenic
GATGAAATGTGGTGGATTAG+TGG	0.498028	5.3:-73044390	MS.gene47721:CDS
GGCACCCACCATAGCATTGT+GGG	0.501789	5.3:-73044955	MS.gene47721:CDS
AGGATTTATTGTGGTAGCAC+TGG	0.506494	5.3:-73043687	MS.gene47721:CDS
GGTTGATAATCCGCTATGTT+GGG	0.513429	5.3:-73043814	MS.gene47721:CDS
TCAAATTCAGGTTCAGGTGG+TGG	0.515057	5.3:+73044158	None:intergenic
CTTGCACTTCATGGAGGCCC+AGG	0.516007	5.3:-73043469	MS.gene47721:CDS
CCATTTCGGCCCATGATACA+AGG	0.516374	5.3:+73044315	None:intergenic
ACTACCACCATTGTTTGAGT+TGG	0.517260	5.3:+73044055	None:intergenic
GTGAAGGATGGATGGAAGGT+GGG	0.517707	5.3:+73044216	None:intergenic
GCATCGTCCAGTTCCTGGAT+TGG	0.519656	5.3:-73043712	MS.gene47721:CDS
TCAAACATCCCACAATGCTA+TGG	0.522391	5.3:+73044947	None:intergenic
GTCATAAAGTGAAGGATGGA+TGG	0.526884	5.3:+73044208	None:intergenic
TTTGTTAATCTACCACCATA+GGG	0.527591	5.3:+73043843	None:intergenic
TCACTAGCCAACTCAAACAA+TGG	0.529140	5.3:-73044062	MS.gene47721:CDS
GGTAGCACTGGTGGAGAAGA+AGG	0.530503	5.3:-73043675	MS.gene47721:CDS
TTTCTTGTGGAAAATGGTTG+CGG	0.534078	5.3:-73044000	MS.gene47721:CDS
ATCCAGATGAAACTGTAGAA+GGG	0.535674	5.3:-73044768	MS.gene47721:CDS
AGTGAAGGATGGATGGAAGG+TGG	0.539276	5.3:+73044215	None:intergenic
ATTTATTGTGGTAGCACTGG+TGG	0.539802	5.3:-73043684	MS.gene47721:CDS
AAACATGTTCCTTGTATCAT+GGG	0.539896	5.3:-73044324	MS.gene47721:CDS
TGGTTGATAATCCGCTATGT+TGG	0.541576	5.3:-73043815	MS.gene47721:CDS
AGAAGAAGGACCAACATTTG+TGG	0.543509	5.3:-73043661	MS.gene47721:CDS
TGAAGTCGTTGCTAATTCCA+TGG	0.543846	5.3:-73044878	MS.gene47721:CDS
ACAAACACTAATATTAATTG+AGG	0.550824	5.3:+73043409	None:intergenic
AATTCAGGTTCAGGTGGTGG+AGG	0.553611	5.3:+73044161	None:intergenic
AATAAGCCTATAATTACTAC+TGG	0.553926	5.3:-73044976	MS.gene47721:CDS
TTTGGAGGCGGCGGTGACAG+AGG	0.555868	5.3:-73043769	MS.gene47721:CDS
GCCCTAAAAGGAATGGTGAC+TGG	0.557544	5.3:-73043521	MS.gene47721:CDS
AGGAGGTAATCCCAAGAGAC+TGG	0.562245	5.3:+73043429	None:intergenic
TGGTAGATTAACAAAAGAGC+TGG	0.565630	5.3:-73043835	MS.gene47721:CDS
AACATCCCACAATGCTATGG+TGG	0.566613	5.3:+73044950	None:intergenic
CTTGGGTTTAATAGATTGGG+TGG	0.568431	5.3:-73043739	MS.gene47721:CDS
CGATTGTTGCAAAGTAACTC+AGG	0.570367	5.3:+73044850	None:intergenic
TCTAAAGAAATGAGGAGCCT+GGG	0.578551	5.3:+73043452	None:intergenic
CCTGAAGATACCACAAATGT+TGG	0.579526	5.3:+73043651	None:intergenic
GATGGAAGGTGGGCGTCGTG+GGG	0.580404	5.3:+73044226	None:intergenic
AGAAGTGACTGTCGAAAACA+AGG	0.582200	5.3:+73044251	None:intergenic
GGAAGCCAATGCTCTTGCAT+TGG	0.585364	5.3:+73045057	None:intergenic
ACAAAGGTTTCCAGAGTCGA+AGG	0.585762	5.3:-73043628	MS.gene47721:CDS
GGTGGAGGTAGATCAGAGAC+GGG	0.587879	5.3:+73044176	None:intergenic
AGCAACGACTTCACCAGCAA+AGG	0.588840	5.3:+73044888	None:intergenic
TAAAGTGAAGGATGGATGGA+AGG	0.590822	5.3:+73044212	None:intergenic
TTGTGGTATCTTCAGGACAA+AGG	0.593564	5.3:-73043644	MS.gene47721:CDS
ACATGTCATAAAGTGAAGGA+TGG	0.594714	5.3:+73044204	None:intergenic
ATGCCAAGCCCTAAAAGGAA+TGG	0.595010	5.3:-73043528	MS.gene47721:CDS
TGGTGGAGGTAGATCAGAGA+CGG	0.600538	5.3:+73044175	None:intergenic
CTCTACATGCACCTTCCTAA+GGG	0.601014	5.3:-73043495	MS.gene47721:CDS
AGTGCAAGTGTCCCTTAGGA+AGG	0.602305	5.3:+73043484	None:intergenic
AACACTAATATTAATTGAGG+AGG	0.603374	5.3:+73043412	None:intergenic
TTGTGGTTGACGGAAGAAAG+AGG	0.605169	5.3:-73043932	MS.gene47721:CDS
GAGTTACTTTGCAACAATCG+TGG	0.605999	5.3:-73044847	MS.gene47721:CDS
GTCCAGTTCCTGGATTGGCA+AGG	0.607030	5.3:-73043707	MS.gene47721:CDS
TTGTTCTTGTGCAGACAACA+TGG	0.607336	5.3:-73044412	MS.gene47721:intron
AATGTGCTTTCGATTCCCTA+TGG	0.610089	5.3:-73043858	MS.gene47721:CDS
GAGACTGGTCTAAAGAAATG+AGG	0.612013	5.3:+73043444	None:intergenic
AGAACACACCGACATCAACA+GGG	0.612828	5.3:+73045007	None:intergenic
AAGTAACTCAGGTTCACCCA+TGG	0.618644	5.3:+73044861	None:intergenic
GTGCTTTCGATTCCCTATGG+TGG	0.619185	5.3:-73043855	MS.gene47721:CDS
AAAATGGGGCTTGTGGTTGA+CGG	0.619932	5.3:-73043942	MS.gene47721:CDS
CTTGTTCCAAAGAAGAAACA+TGG	0.620389	5.3:-73044742	MS.gene47721:CDS
TGGCACCCACCATAGCATTG+TGG	0.626128	5.3:-73044956	MS.gene47721:CDS
ACATCCCACAATGCTATGGT+GGG	0.631579	5.3:+73044951	None:intergenic
ACATACATGTCATAAAGTGA+AGG	0.639724	5.3:+73044200	None:intergenic
ACTTTGCAACAATCGTGGAG+TGG	0.644603	5.3:-73044842	MS.gene47721:CDS
GAAGTCGTTGCTAATTCCAT+GGG	0.644829	5.3:-73044877	MS.gene47721:CDS
GAACACACCGACATCAACAG+GGG	0.646980	5.3:+73045008	None:intergenic
GGATTGGCAAGGATTTATTG+TGG	0.648587	5.3:-73043696	MS.gene47721:CDS
GAGTCAAATTCAGGTTCAGG+TGG	0.648966	5.3:+73044155	None:intergenic
AAATGATAAGATCCAAACAC+AGG	0.657847	5.3:+73043585	None:intergenic
TGATAATCCGCTATGTTGGG+TGG	0.663374	5.3:-73043811	MS.gene47721:CDS
ACAACATGGATGAAATGTGG+TGG	0.668180	5.3:-73044398	MS.gene47721:CDS
AATAGGCATCCGCTATACGA+TGG	0.674136	5.3:+73044351	None:intergenic
GAAATGTGGTGGATTAGTGG+TGG	0.677628	5.3:-73044387	MS.gene47721:CDS
AACACACCGACATCAACAGG+GGG	0.687180	5.3:+73045009	None:intergenic
CAGACAACATGGATGAAATG+TGG	0.692012	5.3:-73044401	MS.gene47721:CDS
CTAGCCAACTCAAACAATGG+TGG	0.694488	5.3:-73044059	MS.gene47721:CDS
TAGCCAGCACTACCTCCCCA+AGG	0.698597	5.3:+73043561	None:intergenic
GGGACACTTGCACTTCATGG+AGG	0.714604	5.3:-73043475	MS.gene47721:CDS
CGATCTGCCACCCAACATAG+CGG	0.715687	5.3:+73043804	None:intergenic
GGAAGAAAGAGGCTAAGCAA+CGG	0.723389	5.3:-73043921	MS.gene47721:CDS
CAACGTGAATATGACCAACG+AGG	0.763069	5.3:-73045100	MS.gene47721:CDS
GTGGAGGTAGATCAGAGACG+GGG	0.788848	5.3:+73044177	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTGAAAAATTCTTTAGCTA+AGG	-	chr5.3:73043616-73043635	MS.gene47721:CDS	20.0%
!!!	AATTTTAATTTTGAACCACT+TGG	-	chr5.3:73044042-73044061	MS.gene47721:CDS	20.0%
!!!	ATCAAAAACTTGTTATCATT+GGG	+	chr5.3:73044261-73044280	None:intergenic	20.0%
!!!	CGATTATGAATTCATATTTT+GGG	-	chr5.3:73044664-73044683	MS.gene47721:intron	20.0%
!	AAAACATTGTTGCAAAAATG+GGG	-	chr5.3:73044589-73044608	MS.gene47721:intron	25.0%
!	AATAAGCCTATAATTACTAC+TGG	-	chr5.3:73043569-73043588	MS.gene47721:CDS	25.0%
!	AGTAAAATGATAAGACCAAT+GGG	+	chr5.3:73043983-73044002	None:intergenic	25.0%
!	AGTATATGCGACTTATTATT+GGG	+	chr5.3:73044431-73044450	None:intergenic	25.0%
!	ATTAAATCAAGGTTTGATAG+TGG	+	chr5.3:73043512-73043531	None:intergenic	25.0%
!	ATTTCTTGGGTTTAATAGAT+TGG	-	chr5.3:73044802-73044821	MS.gene47721:CDS	25.0%
!	GAAAACATTGTTGCAAAAAT+GGG	-	chr5.3:73044588-73044607	MS.gene47721:intron	25.0%
!	TAGTATATGCGACTTATTAT+TGG	+	chr5.3:73044432-73044451	None:intergenic	25.0%
!	TATAATCTATACCATATGCA+AGG	+	chr5.3:73044096-73044115	None:intergenic	25.0%
!	TGAAAACATTGTTGCAAAAA+TGG	-	chr5.3:73044587-73044606	MS.gene47721:intron	25.0%
!	TTAATATCAGAGTCAAATTC+AGG	+	chr5.3:73044402-73044421	None:intergenic	25.0%
!	TTTCTTGGGTTTAATAGATT+GGG	-	chr5.3:73044803-73044822	MS.gene47721:CDS	25.0%
!!	GCGATTATGAATTCATATTT+TGG	-	chr5.3:73044663-73044682	MS.gene47721:intron	25.0%
!!!	GATCAAAAACTTGTTATCAT+TGG	+	chr5.3:73044262-73044281	None:intergenic	25.0%
	AAACATGTTCCTTGTATCAT+GGG	-	chr5.3:73044221-73044240	MS.gene47721:CDS	30.0%
	AAATGATAAGATCCAAACAC+AGG	+	chr5.3:73044963-73044982	None:intergenic	30.0%
	ACATACATGTCATAAAGTGA+AGG	+	chr5.3:73044348-73044367	None:intergenic	30.0%
	ATTAGTACTTGTGTTTAGAC+AGG	-	chr5.3:73043948-73043967	MS.gene47721:CDS	30.0%
	CAGTAAAATGATAAGACCAA+TGG	+	chr5.3:73043984-73044003	None:intergenic	30.0%
	TTTGTTAATCTACCACCATA+GGG	+	chr5.3:73044705-73044724	None:intergenic	30.0%
!	ACCAAAACATTTTGTTCTCA+AGG	-	chr5.3:73043864-73043883	MS.gene47721:CDS	30.0%
!	CCAAAACATTTTGTTCTCAA+GGG	-	chr5.3:73043865-73043884	MS.gene47721:CDS	30.0%
!	TGGATCTTATCATTTTCCTT+GGG	-	chr5.3:73044968-73044987	MS.gene47721:CDS	30.0%
!	TTAATTTTGAACCACTTGGT+TGG	-	chr5.3:73044046-73044065	MS.gene47721:CDS	30.0%
!	TTGGATCTTATCATTTTCCT+TGG	-	chr5.3:73044967-73044986	MS.gene47721:CDS	30.0%
!	TTTTGTTAATCTACCACCAT+AGG	+	chr5.3:73044706-73044725	None:intergenic	30.0%
!!	GTTTTCATTATCGATTGAAG+CGG	+	chr5.3:73044574-73044593	None:intergenic	30.0%
	AAGGAACATGTTTGCAGAAT+AGG	+	chr5.3:73044214-73044233	None:intergenic	35.0%
	AATCCAGATGAAACTGTAGA+AGG	-	chr5.3:73043776-73043795	MS.gene47721:CDS	35.0%
	AATCTATACCATATGCAAGG+TGG	+	chr5.3:73044093-73044112	None:intergenic	35.0%
	AATGATAAGACCAATGGGTA+CGG	+	chr5.3:73043978-73043997	None:intergenic	35.0%
	ACATGTCATAAAGTGAAGGA+TGG	+	chr5.3:73044344-73044363	None:intergenic	35.0%
	ATCCAGATGAAACTGTAGAA+GGG	-	chr5.3:73043777-73043796	MS.gene47721:CDS	35.0%
	ATTTCTTTAGACCAGTCTCT+TGG	-	chr5.3:73045105-73045124	MS.gene47721:CDS	35.0%
	CAAACATGTTCCTTGTATCA+TGG	-	chr5.3:73044220-73044239	MS.gene47721:CDS	35.0%
	CTTGTTCCAAAGAAGAAACA+TGG	-	chr5.3:73043803-73043822	MS.gene47721:CDS	35.0%
	GTTTCTTATTATGCCTTTGC+TGG	-	chr5.3:73043644-73043663	MS.gene47721:CDS	35.0%
	TCTCAAGGGAATATTCGAAT+AGG	-	chr5.3:73043879-73043898	MS.gene47721:CDS	35.0%
	TGGTAGATTAACAAAAGAGC+TGG	-	chr5.3:73044710-73044729	MS.gene47721:intron	35.0%
	TTCTGCTTTCTTGTGGAAAA+TGG	-	chr5.3:73044539-73044558	MS.gene47721:intron	35.0%
	TTTCCACAAGAAAGCAGAAA+AGG	+	chr5.3:73044538-73044557	None:intergenic	35.0%
	TTTCTTGTGGAAAATGGTTG+CGG	-	chr5.3:73044545-73044564	MS.gene47721:intron	35.0%
	TTTCTTTAGACCAGTCTCTT+GGG	-	chr5.3:73045106-73045125	MS.gene47721:CDS	35.0%
!	AGATCGAGTTCATGAGTTTT+TGG	-	chr5.3:73044758-73044777	MS.gene47721:CDS	35.0%
!	CCCTTGAGAACAAAATGTTT+TGG	+	chr5.3:73043868-73043887	None:intergenic	35.0%
!	GGATCTTATCATTTTCCTTG+GGG	-	chr5.3:73044969-73044988	MS.gene47721:CDS	35.0%
!!!	TGGTTTAGTGGTGTTGTTTT+GGG	-	chr5.3:73044066-73044085	MS.gene47721:CDS	35.0%
!!!	TTGGTTTAGTGGTGTTGTTT+TGG	-	chr5.3:73044065-73044084	MS.gene47721:CDS	35.0%
	AATGTGCTTTCGATTCCCTA+TGG	-	chr5.3:73044687-73044706	MS.gene47721:intron	40.0%
	ACAACATGGATGAAATGTGG+TGG	-	chr5.3:73044147-73044166	MS.gene47721:CDS	40.0%
	ACTACCACCATTGTTTGAGT+TGG	+	chr5.3:73044493-73044512	None:intergenic	40.0%
	AGAAGAAGGACCAACATTTG+TGG	-	chr5.3:73044884-73044903	MS.gene47721:CDS	40.0%
	AGAAGTGACTGTCGAAAACA+AGG	+	chr5.3:73044297-73044316	None:intergenic	40.0%
	AGCACACCATGTTTCTTCTT+TGG	+	chr5.3:73043812-73043831	None:intergenic	40.0%
	AGGATTTATTGTGGTAGCAC+TGG	-	chr5.3:73044858-73044877	MS.gene47721:CDS	40.0%
	CAATAAATCCTTGCCAATCC+AGG	+	chr5.3:73044849-73044868	None:intergenic	40.0%
	CAGACAACATGGATGAAATG+TGG	-	chr5.3:73044144-73044163	MS.gene47721:CDS	40.0%
	CAGGGGGAAGAATTAAATCA+AGG	+	chr5.3:73043523-73043542	None:intergenic	40.0%
	CCAACATTTGTGGTATCTTC+AGG	-	chr5.3:73044894-73044913	MS.gene47721:CDS	40.0%
	CCTGAAGATACCACAAATGT+TGG	+	chr5.3:73044897-73044916	None:intergenic	40.0%
	CGATTGTTGCAAAGTAACTC+AGG	+	chr5.3:73043698-73043717	None:intergenic	40.0%
	CTTGGGTTTAATAGATTGGG+TGG	-	chr5.3:73044806-73044825	MS.gene47721:CDS	40.0%
	GAAAACAAGGTTGCGTTGTT+GGG	+	chr5.3:73044284-73044303	None:intergenic	40.0%
	GAAGTCGTTGCTAATTCCAT+GGG	-	chr5.3:73043668-73043687	MS.gene47721:CDS	40.0%
	GTCATAAAGTGAAGGATGGA+TGG	+	chr5.3:73044340-73044359	None:intergenic	40.0%
	TAAAGTGAAGGATGGATGGA+AGG	+	chr5.3:73044336-73044355	None:intergenic	40.0%
	TATGACCAACGAGGAAATAG+TGG	-	chr5.3:73043454-73043473	MS.gene47721:CDS	40.0%
	TCAAACATCCCACAATGCTA+TGG	+	chr5.3:73043601-73043620	None:intergenic	40.0%
	TCACTAGCCAACTCAAACAA+TGG	-	chr5.3:73044483-73044502	MS.gene47721:intron	40.0%
	TCTAAAGAAATGAGGAGCCT+GGG	+	chr5.3:73045096-73045115	None:intergenic	40.0%
	TGAAGTCGTTGCTAATTCCA+TGG	-	chr5.3:73043667-73043686	MS.gene47721:CDS	40.0%
	TGGGTGCCAGTAGTAATTAT+AGG	+	chr5.3:73043578-73043597	None:intergenic	40.0%
	TTCCCTTCTACAGTTTCATC+TGG	+	chr5.3:73043782-73043801	None:intergenic	40.0%
	TTGTGGTATCTTCAGGACAA+AGG	-	chr5.3:73044901-73044920	MS.gene47721:CDS	40.0%
	TTGTTCTTGTGCAGACAACA+TGG	-	chr5.3:73044133-73044152	MS.gene47721:CDS	40.0%
!	AACCAGTCACCATTCCTTTT+AGG	+	chr5.3:73045029-73045048	None:intergenic	40.0%
!	ACCAGTCACCATTCCTTTTA+GGG	+	chr5.3:73045028-73045047	None:intergenic	40.0%
!	AGTCCTTTTCTGCTTTCTTG+TGG	-	chr5.3:73044532-73044551	MS.gene47721:intron	40.0%
!	ATTTATTGTGGTAGCACTGG+TGG	-	chr5.3:73044861-73044880	MS.gene47721:CDS	40.0%
!	CACTTTTGACCATCGTATAG+CGG	-	chr5.3:73044185-73044204	MS.gene47721:CDS	40.0%
!	GAGTTACTTTGCAACAATCG+TGG	-	chr5.3:73043698-73043717	MS.gene47721:CDS	40.0%
!	GGATTGGCAAGGATTTATTG+TGG	-	chr5.3:73044849-73044868	MS.gene47721:CDS	40.0%
!	TCAGAGTCAAATTCAGGTTC+AGG	+	chr5.3:73044396-73044415	None:intergenic	40.0%
!	TCTTATCATTTTCCTTGGGG+AGG	-	chr5.3:73044972-73044991	MS.gene47721:CDS	40.0%
!!	AGTCGAAGGTTGATTTTGGA+TGG	-	chr5.3:73044931-73044950	MS.gene47721:CDS	40.0%
!!	AGTTCATGAGTTTTTGGAGG+CGG	-	chr5.3:73044764-73044783	MS.gene47721:CDS	40.0%
!!	GAGACTGGTCTAAAGAAATG+AGG	+	chr5.3:73045104-73045123	None:intergenic	40.0%
!!	GATGAAATGTGGTGGATTAG+TGG	-	chr5.3:73044155-73044174	MS.gene47721:CDS	40.0%
!!	GGTTGATAATCCGCTATGTT+GGG	-	chr5.3:73044731-73044750	MS.gene47721:CDS	40.0%
!!	GTCGAAGGTTGATTTTGGAT+GGG	-	chr5.3:73044932-73044951	MS.gene47721:CDS	40.0%
!!	TCGAAGGTTGATTTTGGATG+GGG	-	chr5.3:73044933-73044952	MS.gene47721:CDS	40.0%
!!	TCGAGTTCATGAGTTTTTGG+AGG	-	chr5.3:73044761-73044780	MS.gene47721:CDS	40.0%
!!	TGGTTGATAATCCGCTATGT+TGG	-	chr5.3:73044730-73044749	MS.gene47721:CDS	40.0%
	AAAATGGGGCTTGTGGTTGA+CGG	-	chr5.3:73044603-73044622	MS.gene47721:intron	45.0%
	AACATCCCACAATGCTATGG+TGG	+	chr5.3:73043598-73043617	None:intergenic	45.0%
	AAGAACACACCGACATCAAC+AGG	+	chr5.3:73043542-73043561	None:intergenic	45.0%
	AAGTAACTCAGGTTCACCCA+TGG	+	chr5.3:73043687-73043706	None:intergenic	45.0%
	AATAGGCATCCGCTATACGA+TGG	+	chr5.3:73044197-73044216	None:intergenic	45.0%
	ACAAAGGTTTCCAGAGTCGA+AGG	-	chr5.3:73044917-73044936	MS.gene47721:CDS	45.0%
	ACACCACTAAACCAACCAAG+TGG	+	chr5.3:73044060-73044079	None:intergenic	45.0%
	ACATCCCACAATGCTATGGT+GGG	+	chr5.3:73043597-73043616	None:intergenic	45.0%
	ACTTTGCAACAATCGTGGAG+TGG	-	chr5.3:73043703-73043722	MS.gene47721:CDS	45.0%
	AGAACACACCGACATCAACA+GGG	+	chr5.3:73043541-73043560	None:intergenic	45.0%
	ATCATTGGGCTGAGCCATTT+CGG	+	chr5.3:73044247-73044266	None:intergenic	45.0%
	ATGAAGTGCAAGTGTCCCTT+AGG	+	chr5.3:73045068-73045087	None:intergenic	45.0%
	ATGCCAAGCCCTAAAAGGAA+TGG	-	chr5.3:73045017-73045036	MS.gene47721:CDS	45.0%
	ATTCTTCCCCCTGTTGATGT+CGG	-	chr5.3:73043530-73043549	MS.gene47721:CDS	45.0%
	CAACGTGAATATGACCAACG+AGG	-	chr5.3:73043445-73043464	MS.gene47721:CDS	45.0%
	CCAAAATCAACCTTCGACTC+TGG	+	chr5.3:73044930-73044949	None:intergenic	45.0%
	CGAAAACAAGGTTGCGTTGT+TGG	+	chr5.3:73044285-73044304	None:intergenic	45.0%
	CTAGCCAACTCAAACAATGG+TGG	-	chr5.3:73044486-73044505	MS.gene47721:intron	45.0%
	CTCTACATGCACCTTCCTAA+GGG	-	chr5.3:73045050-73045069	MS.gene47721:CDS	45.0%
	GGAAGAAAGAGGCTAAGCAA+CGG	-	chr5.3:73044624-73044643	MS.gene47721:intron	45.0%
	GTCTAAAGAAATGAGGAGCC+TGG	+	chr5.3:73045097-73045116	None:intergenic	45.0%
	TAAGGGACACTTGCACTTCA+TGG	-	chr5.3:73045067-73045086	MS.gene47721:CDS	45.0%
	TGATAATCCGCTATGTTGGG+TGG	-	chr5.3:73044734-73044753	MS.gene47721:CDS	45.0%
	TGTTGCAAAAATGGGGCTTG+TGG	-	chr5.3:73044596-73044615	MS.gene47721:intron	45.0%
	TTGTGGTTGACGGAAGAAAG+AGG	-	chr5.3:73044613-73044632	MS.gene47721:intron	45.0%
!	GAGTCAAATTCAGGTTCAGG+TGG	+	chr5.3:73044393-73044412	None:intergenic	45.0%
!	TCAAATTCAGGTTCAGGTGG+TGG	+	chr5.3:73044390-73044409	None:intergenic	45.0%
!!	CCAGAGTCGAAGGTTGATTT+TGG	-	chr5.3:73044927-73044946	MS.gene47721:CDS	45.0%
!!	CGAAGGTTGATTTTGGATGG+GGG	-	chr5.3:73044934-73044953	MS.gene47721:CDS	45.0%
!!	GAAATGTGGTGGATTAGTGG+TGG	-	chr5.3:73044158-73044177	MS.gene47721:CDS	45.0%
!!	GAACCACTTGGTTGGTTTAG+TGG	-	chr5.3:73044054-73044073	MS.gene47721:CDS	45.0%
!!	GTGACAGAGGAGCATTTCTT+GGG	-	chr5.3:73044789-73044808	MS.gene47721:CDS	45.0%
!!!	TCACCATTCCTTTTAGGGCT+TGG	+	chr5.3:73045023-73045042	None:intergenic	45.0%
!!!	TTCCTTTTAGGGCTTGGCAT+TGG	+	chr5.3:73045017-73045036	None:intergenic	45.0%
!!!	TTTTGGGACCACCTTGCATA+TGG	-	chr5.3:73044082-73044101	MS.gene47721:CDS	45.0%
	AACACACCGACATCAACAGG+GGG	+	chr5.3:73043539-73043558	None:intergenic	50.0%
	AGCAACGACTTCACCAGCAA+AGG	+	chr5.3:73043660-73043679	None:intergenic	50.0%
	AGCTGCCACTATTTCCTCGT+TGG	+	chr5.3:73043462-73043481	None:intergenic	50.0%
	AGGAGGTAATCCCAAGAGAC+TGG	+	chr5.3:73045119-73045138	None:intergenic	50.0%
	AGTGAAGGATGGATGGAAGG+TGG	+	chr5.3:73044333-73044352	None:intergenic	50.0%
	AGTGCAAGTGTCCCTTAGGA+AGG	+	chr5.3:73045064-73045083	None:intergenic	50.0%
	ATCCTTGCCAATCCAGGAAC+TGG	+	chr5.3:73044843-73044862	None:intergenic	50.0%
	CCATTTCGGCCCATGATACA+AGG	+	chr5.3:73044233-73044252	None:intergenic	50.0%
	CCTTGTATCATGGGCCGAAA+TGG	-	chr5.3:73044230-73044249	MS.gene47721:CDS	50.0%
	GAACACACCGACATCAACAG+GGG	+	chr5.3:73043540-73043559	None:intergenic	50.0%
	GCCCTAAAAGGAATGGTGAC+TGG	-	chr5.3:73045024-73045043	MS.gene47721:CDS	50.0%
	GCTCTACATGCACCTTCCTA+AGG	-	chr5.3:73045049-73045068	MS.gene47721:CDS	50.0%
	GGAAGCCAATGCTCTTGCAT+TGG	+	chr5.3:73043491-73043510	None:intergenic	50.0%
	GTGAAGGATGGATGGAAGGT+GGG	+	chr5.3:73044332-73044351	None:intergenic	50.0%
	GTGCTTTCGATTCCCTATGG+TGG	-	chr5.3:73044690-73044709	MS.gene47721:intron	50.0%
	TGCCAATGCCAAGCCCTAAA+AGG	-	chr5.3:73045012-73045031	MS.gene47721:CDS	50.0%
	TGGTGGAGGTAGATCAGAGA+CGG	+	chr5.3:73044373-73044392	None:intergenic	50.0%
!	AATTCAGGTTCAGGTGGTGG+AGG	+	chr5.3:73044387-73044406	None:intergenic	50.0%
!	CGCTACCAATGCAAGAGCAT+TGG	-	chr5.3:73043483-73043502	MS.gene47721:CDS	50.0%
!!	ACATGGTGTGCTTGCTGTTC+AGG	-	chr5.3:73043820-73043839	MS.gene47721:CDS	50.0%
!!	GGTGACAGAGGAGCATTTCT+TGG	-	chr5.3:73044788-73044807	MS.gene47721:CDS	50.0%
!!	TCATGAGTTTTTGGAGGCGG+CGG	-	chr5.3:73044767-73044786	MS.gene47721:CDS	50.0%
	CGATCTGCCACCCAACATAG+CGG	+	chr5.3:73044744-73044763	None:intergenic	55.0%
	GCATCGTCCAGTTCCTGGAT+TGG	-	chr5.3:73044833-73044852	MS.gene47721:CDS	55.0%
	GGCACCCACCATAGCATTGT+GGG	-	chr5.3:73043590-73043609	MS.gene47721:CDS	55.0%
	GGGACACTTGCACTTCATGG+AGG	-	chr5.3:73045070-73045089	MS.gene47721:CDS	55.0%
	GGTGGAGGTAGATCAGAGAC+GGG	+	chr5.3:73044372-73044391	None:intergenic	55.0%
	GTGGAGGTAGATCAGAGACG+GGG	+	chr5.3:73044371-73044390	None:intergenic	55.0%
	TGGCACCCACCATAGCATTG+TGG	-	chr5.3:73043589-73043608	MS.gene47721:CDS	55.0%
	TTTCCTTGGGGAGGTAGTGC+TGG	-	chr5.3:73044981-73045000	MS.gene47721:CDS	55.0%
!!	GAAGAGCATCGTCCAGTTCC+TGG	-	chr5.3:73044828-73044847	MS.gene47721:CDS	55.0%
!!	GGTAGCACTGGTGGAGAAGA+AGG	-	chr5.3:73044870-73044889	MS.gene47721:CDS	55.0%
!!	GTCCAGTTCCTGGATTGGCA+AGG	-	chr5.3:73044838-73044857	MS.gene47721:CDS	55.0%
!!!	GGACTCGAGTTTTGAGCGCT+TGG	+	chr5.3:73044517-73044536	None:intergenic	55.0%
	CTTGCACTTCATGGAGGCCC+AGG	-	chr5.3:73045076-73045095	MS.gene47721:CDS	60.0%
	GACAGGCACTCCGTACCCAT+TGG	-	chr5.3:73043965-73043984	MS.gene47721:CDS	60.0%
	GGATGGGGGAAGCCTGTGTT+TGG	-	chr5.3:73044948-73044967	MS.gene47721:CDS	60.0%
	TAGCCAGCACTACCTCCCCA+AGG	+	chr5.3:73044987-73045006	None:intergenic	60.0%
	TTTGGAGGCGGCGGTGACAG+AGG	-	chr5.3:73044776-73044795	MS.gene47721:CDS	65.0%
!	GATGGAAGGTGGGCGTCGTG+GGG	+	chr5.3:73044322-73044341	None:intergenic	65.0%
!	GGATGGAAGGTGGGCGTCGT+GGG	+	chr5.3:73044323-73044342	None:intergenic	65.0%
!	TGGATGGAAGGTGGGCGTCG+TGG	+	chr5.3:73044324-73044343	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	73043422	73045145	73043422	ID=MS.gene47721
chr5.3	mRNA	73043422	73045145	73043422	ID=MS.gene47721.t1;Parent=MS.gene47721
chr5.3	exon	73044726	73045145	73044726	ID=MS.gene47721.t1.exon1;Parent=MS.gene47721.t1
chr5.3	CDS	73044726	73045145	73044726	ID=cds.MS.gene47721.t1;Parent=MS.gene47721.t1
chr5.3	exon	73043422	73044420	73043422	ID=MS.gene47721.t1.exon2;Parent=MS.gene47721.t1
chr5.3	CDS	73043422	73044420	73043422	ID=cds.MS.gene47721.t1;Parent=MS.gene47721.t1

Gene Sequence

>MS.gene47721

ATGTGTATTGGAAAAGTAGAACTCAACGTGAATATGACCAACGAGGAAATAGTGGCAGCTGCGCTACCAATGCAAGAGCATTGGCTTCCACTATCAAACCTTGATTTAATTCTTCCCCCTGTTGATGTCGGTGTGTTCTTTTGCTACAATAAGCCTATAATTACTACTGGCACCCACCATAGCATTGTGGGATGTTTGAAAAATTCTTTAGCTAAGGCTCTTGTTTCTTATTATGCCTTTGCTGGTGAAGTCGTTGCTAATTCCATGGGTGAACCTGAGTTACTTTGCAACAATCGTGGAGTGGATTTTGTTGAAGCTTTTGCTGATGTTGAGTTACAATCTCTAAATTTGTATAATCCAGATGAAACTGTAGAAGGGAAGCTTGTTCCAAAGAAGAAACATGGTGTGCTTGCTGTTCAGGTGAAAAAAGATTTATTTTATTACCAAAACATTTTGTTCTCAAGGGAATATTCGAATAGGCAAAGAATTATTATAAGCATGTACTATTATAATAATCATTATAATTATTAGTACTTGTGTTTAGACAGGCACTCCGTACCCATTGGTCTTATCATTTTACTGCACTGATGCGAATAAATTACGAACAATAATTTTTATTAAATTTTAATTTTGAACCACTTGGTTGGTTTAGTGGTGTTGTTTTGGGACCACCTTGCATATGGTATAGATTATAAAGTGTTTTAATCGTCATTGTTCTTGTGCAGACAACATGGATGAAATGTGGTGGATTAGTGGTGGCTTGCACTTTTGACCATCGTATAGCGGATGCCTATTCTGCAAACATGTTCCTTGTATCATGGGCCGAAATGGCTCAGCCCAATGATAACAAGTTTTTGATCCCAACAACGCAACCTTGTTTTCGACAGTCACTTCTAACCCCACGACGCCCACCTTCCATCCATCCTTCACTTTATGACATGTATGTCCCCGTCTCTGATCTACCTCCACCACCTGAACCTGAATTTGACTCTGATATTAAAACTAACCCAATAATAAGTCGCATATACTATGTAACAAGCAAAGAACTCAACAACATGCAATCACTAGCCAACTCAAACAATGGTGGTAGTTCCAAGCGCTCAAAACTCGAGTCCTTTTCTGCTTTCTTGTGGAAAATGGTTGCGGAAGCCGCTTCAATCGATAATGAAAACATTGTTGCAAAAATGGGGCTTGTGGTTGACGGAAGAAAGAGGCTAAGCAACGGTGACAAAAACAAAGAAGCGATTATGAATTCATATTTTGGGAATGTGCTTTCGATTCCCTATGGTGGTAGATTAACAAAAGAGCTGGTTGATAATCCGCTATGTTGGGTGGCAGATCGAGTTCATGAGTTTTTGGAGGCGGCGGTGACAGAGGAGCATTTCTTGGGTTTAATAGATTGGGTGGAAGAGCATCGTCCAGTTCCTGGATTGGCAAGGATTTATTGTGGTAGCACTGGTGGAGAAGAAGGACCAACATTTGTGGTATCTTCAGGACAAAGGTTTCCAGAGTCGAAGGTTGATTTTGGATGGGGGAAGCCTGTGTTTGGATCTTATCATTTTCCTTGGGGAGGTAGTGCTGGCTATGTTATGCCAATGCCAAGCCCTAAAAGGAATGGTGACTGGTTGCTCTACATGCACCTTCCTAAGGGACACTTGCACTTCATGGAGGCCCAGGCTCCTCATTTCTTTAGACCAGTCTCTTGGGATTACCTCCTCAATTAA

Protein sequence

>MS.gene47721.t1

MCIGKVELNVNMTNEEIVAAALPMQEHWLPLSNLDLILPPVDVGVFFCYNKPIITTGTHHSIVGCLKNSLAKALVSYYAFAGEVVANSMGEPELLCNNRGVDFVEAFADVELQSLNLYNPDETVEGKLVPKKKHGVLAVQTTWMKCGGLVVACTFDHRIADAYSANMFLVSWAEMAQPNDNKFLIPTTQPCFRQSLLTPRRPPSIHPSLYDMYVPVSDLPPPPEPEFDSDIKTNPIISRIYYVTSKELNNMQSLANSNNGGSSKRSKLESFSAFLWKMVAEAASIDNENIVAKMGLVVDGRKRLSNGDKNKEAIMNSYFGNVLSIPYGGRLTKELVDNPLCWVADRVHEFLEAAVTEEHFLGLIDWVEEHRPVPGLARIYCGSTGGEEGPTFVVSSGQRFPESKVDFGWGKPVFGSYHFPWGGSAGYVMPMPSPKRNGDWLLYMHLPKGHLHFMEAQAPHFFRPVSWDYLLN