AlfalfaGEDB | MS.gene48023

M. sativa cultivar XinJiangDaYe / MS.gene48023

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48023.t1	XP_003617368.1	93.7	473	22	3	1	467	1	471	1.10E-263	918.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48023.t1	Q9FI78	25.2	457	294	16	10	450	3	427	3.6e-29	131.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48023.t1	G7K3A2	93.7	473	22	3	1	467	1	471	7.9e-264	918.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04926	MS.gene48023	0.809898	1.51E-50	-1.69E-46
MS.gene049549	MS.gene48023	0.835455	1.66E-56	-1.69E-46
MS.gene050329	MS.gene48023	0.81619	6.28E-52	-1.69E-46
MS.gene050479	MS.gene48023	0.801382	9.25E-49	-1.69E-46
MS.gene050547	MS.gene48023	0.802323	5.93E-49	-1.69E-46
MS.gene050647	MS.gene48023	0.805373	1.38E-49	-1.69E-46
MS.gene051440	MS.gene48023	0.800799	1.22E-48	-1.69E-46
MS.gene052211	MS.gene48023	0.857642	1.42E-62	-1.69E-46
MS.gene052212	MS.gene48023	0.823637	1.24E-53	-1.69E-46
MS.gene05240	MS.gene48023	0.846642	1.91E-59	-1.69E-46
MS.gene052606	MS.gene48023	0.809573	1.77E-50	-1.69E-46
MS.gene053031	MS.gene48023	0.809253	2.07E-50	-1.69E-46
MS.gene053406	MS.gene48023	0.858159	9.98E-63	-1.69E-46
MS.gene053887	MS.gene48023	0.801963	7.03E-49	-1.69E-46
MS.gene054146	MS.gene48023	0.863985	1.68E-64	-1.69E-46
MS.gene054147	MS.gene48023	0.83141	1.70E-55	-1.69E-46
MS.gene054277	MS.gene48023	0.850472	1.66E-60	-1.69E-46
MS.gene054742	MS.gene48023	0.855066	8.10E-62	-1.69E-46
MS.gene055124	MS.gene48023	0.804694	1.91E-49	-1.69E-46
MS.gene055240	MS.gene48023	0.836726	7.90E-57	-1.69E-46
MS.gene055340	MS.gene48023	0.810756	9.84E-51	-1.69E-46
MS.gene056076	MS.gene48023	0.802597	5.20E-49	-1.69E-46
MS.gene056218	MS.gene48023	0.802554	5.31E-49	-1.69E-46
MS.gene056590	MS.gene48023	0.804091	2.55E-49	-1.69E-46
MS.gene056591	MS.gene48023	0.801084	1.06E-48	-1.69E-46
MS.gene056680	MS.gene48023	0.801572	8.45E-49	-1.69E-46
MS.gene056748	MS.gene48023	0.807585	4.70E-50	-1.69E-46
MS.gene056920	MS.gene48023	0.846233	2.47E-59	-1.69E-46
MS.gene056922	MS.gene48023	0.811365	7.26E-51	-1.69E-46
MS.gene057547	MS.gene48023	0.801499	8.75E-49	-1.69E-46
MS.gene057550	MS.gene48023	0.801301	9.61E-49	-1.69E-46
MS.gene058177	MS.gene48023	0.800367	1.49E-48	-1.69E-46
MS.gene058381	MS.gene48023	0.821349	4.23E-53	-1.69E-46
MS.gene058603	MS.gene48023	0.827971	1.16E-54	-1.69E-46
MS.gene059060	MS.gene48023	0.803196	3.91E-49	-1.69E-46
MS.gene059955	MS.gene48023	0.80108	1.07E-48	-1.69E-46
MS.gene060087	MS.gene48023	0.85268	3.94E-61	-1.69E-46
MS.gene060358	MS.gene48023	0.80074	1.25E-48	-1.69E-46
MS.gene060593	MS.gene48023	0.802456	5.56E-49	-1.69E-46
MS.gene061038	MS.gene48023	0.809054	2.29E-50	-1.69E-46
MS.gene061092	MS.gene48023	0.826004	3.44E-54	-1.69E-46
MS.gene061094	MS.gene48023	0.8246	7.39E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene48023	MS.gene32938	PPI
MS.gene017058	MS.gene48023	PPI
MS.gene48023	MS.gene012965	PPI
MS.gene48023	MS.gene017058	PPI
MS.gene48023	MS.gene32936	PPI
MS.gene48023	MS.gene38703	PPI
MS.gene48023	MS.gene039597	PPI
MS.gene48023	MS.gene38700	PPI
MS.gene48023	MS.gene38365	PPI
MS.gene48023	MS.gene47716	PPI
MS.gene48023	MS.gene32933	PPI
MS.gene72785	MS.gene48023	PPI
MS.gene48023	MS.gene38362	PPI
MS.gene48023	MS.gene038274	PPI
MS.gene48023	MS.gene38361	PPI
MS.gene48021	MS.gene48023	PPI
MS.gene48023	MS.gene38706	PPI
MS.gene48023	MS.gene48021	PPI
MS.gene48023	MS.gene38363	PPI
MS.gene48023	MS.gene38701	PPI
MS.gene48023	MS.gene69496	PPI
MS.gene48023	MS.gene38359	PPI
MS.gene48023	MS.gene017054	PPI
MS.gene48023	MS.gene019178	PPI
MS.gene48023	MS.gene38699	PPI
MS.gene48023	MS.gene83461	PPI
MS.gene48023	MS.gene028134	PPI
MS.gene48023	MS.gene47721	PPI
MS.gene48023	MS.gene012959	PPI
MS.gene48023	MS.gene48027	PPI
MS.gene48023	MS.gene012960	PPI
MS.gene48023	MS.gene012957	PPI
MS.gene48023	MS.gene47719	PPI
MS.gene48023	MS.gene82145	PPI
MS.gene48023	MS.gene069321	PPI
MS.gene48023	MS.gene000507	PPI
MS.gene48023	MS.gene95242	PPI
MS.gene48023	MS.gene038271	PPI
MS.gene48023	MS.gene38702	PPI
MS.gene48023	MS.gene32934	PPI
MS.gene48023	MS.gene046246	PPI
MS.gene48023	MS.gene38704	PPI
MS.gene48023	MS.gene22199	PPI
MS.gene48023	MS.gene017053	PPI
MS.gene48023	MS.gene012961	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48023.t1	MTR_5g090830	95.137	473	15	3	1	467	1	471	0.0	928
MS.gene48023.t1	MTR_5g090810	92.600	473	27	3	1	467	1	471	0.0	911
MS.gene48023.t1	MTR_5g090880	80.127	473	88	2	1	467	1	473	0.0	774
MS.gene48023.t1	MTR_3g450710	65.481	478	142	8	1	465	1	468	0.0	615
MS.gene48023.t1	MTR_3g450690	63.125	480	154	7	1	465	1	472	0.0	602
MS.gene48023.t1	MTR_5g026320	34.475	438	254	13	7	435	15	428	1.59e-69	228
MS.gene48023.t1	MTR_8g096530	28.733	442	253	20	31	453	28	426	8.00e-32	127
MS.gene48023.t1	MTR_8g075610	25.858	437	268	17	32	450	16	414	5.07e-31	124
MS.gene48023.t1	MTR_2g064495	25.597	461	286	19	10	450	3	426	1.94e-30	122
MS.gene48023.t1	MTR_2g105330	25.597	461	286	19	10	450	3	426	9.87e-30	120
MS.gene48023.t1	MTR_4g007540	23.841	453	297	14	10	446	3	423	1.61e-28	117
MS.gene48023.t1	MTR_4g097090	25.647	464	293	15	23	460	19	456	3.22e-28	117
MS.gene48023.t1	MTR_7g114470	26.651	424	252	15	8	410	9	394	4.19e-28	116
MS.gene48023.t1	MTR_3g098980	27.041	392	235	14	31	408	35	389	6.54e-28	115
MS.gene48023.t1	MTR_3g049150	26.424	439	265	18	31	441	23	431	1.03e-27	115
MS.gene48023.t1	MTR_7g114560	24.672	458	283	13	8	442	8	426	1.32e-27	115
MS.gene48023.t1	MTR_7g114550	24.672	458	283	13	8	442	8	426	5.86e-27	113
MS.gene48023.t1	MTR_1g040215	26.434	401	229	14	63	431	61	427	5.92e-27	113
MS.gene48023.t1	MTR_4g127650	23.871	465	299	13	23	460	20	456	1.01e-26	112
MS.gene48023.t1	MTR_5g076700	25.055	455	286	16	8	442	8	427	3.16e-26	111
MS.gene48023.t1	MTR_7g027175	26.463	393	239	14	31	409	37	393	7.13e-26	109
MS.gene48023.t1	MTR_1g040175	25.558	403	230	13	63	431	61	427	7.81e-25	107
MS.gene48023.t1	MTR_7g086000	28.000	375	211	16	66	407	60	408	1.32e-24	106
MS.gene48023.t1	MTR_1g081660	26.667	390	230	16	47	419	57	407	3.45e-24	105
MS.gene48023.t1	MTR_7g114490	23.881	469	295	14	8	453	8	437	3.94e-24	105
MS.gene48023.t1	MTR_7g114520	25.764	458	278	18	8	441	9	428	1.31e-23	103
MS.gene48023.t1	MTR_3g026950	23.649	444	293	11	31	450	23	444	2.97e-21	96.3
MS.gene48023.t1	MTR_1g053315	24.079	407	250	14	29	410	40	412	3.73e-21	96.3
MS.gene48023.t1	MTR_7g085990	25.134	374	235	14	66	409	55	413	3.60e-20	93.2
MS.gene48023.t1	MTR_7g085930	27.342	395	230	19	66	424	59	432	8.92e-20	92.0
MS.gene48023.t1	MTR_1g040195	24.796	367	210	12	96	431	71	402	2.47e-18	87.4
MS.gene48023.t1	MTR_3g101900	22.683	410	258	10	27	408	19	397	9.99e-18	85.9
MS.gene48023.t1	MTR_1g040320	23.821	403	201	14	63	431	61	391	1.57e-17	85.1
MS.gene48023.t1	MTR_2g015430	23.940	401	255	16	24	408	18	384	1.74e-17	85.1
MS.gene48023.t1	MTR_7g056087	24.401	459	293	18	10	447	4	429	6.46e-17	83.2
MS.gene48023.t1	MTR_4g055170	24.176	455	292	18	17	446	19	445	8.28e-17	82.8
MS.gene48023.t1	MTR_7g084940	24.823	423	257	14	7	409	13	394	1.04e-16	82.4
MS.gene48023.t1	MTR_3g086020	24.362	431	250	19	7	421	2	372	1.65e-16	81.6
MS.gene48023.t1	MTR_7g084910	24.783	460	254	19	7	429	14	418	2.07e-16	81.6
MS.gene48023.t1	MTR_7g114510	25.091	275	174	9	137	410	9	252	9.36e-16	78.6
MS.gene48023.t1	MTR_3g465990	23.894	339	212	11	49	365	50	364	1.49e-14	75.9
MS.gene48023.t1	MTR_3g101960	23.270	318	198	12	49	343	48	342	3.07e-14	75.1
MS.gene48023.t1	MTR_3g088420	24.365	394	237	15	36	408	32	385	7.19e-14	73.9
MS.gene48023.t1	MTR_8g079980	22.819	447	277	17	10	434	3	403	1.06e-13	73.2
MS.gene48023.t1	MTR_4g094842	24.074	378	221	13	61	408	57	398	1.46e-13	72.8
MS.gene48023.t1	MTR_3g101930	22.251	382	246	11	49	406	49	403	1.55e-13	72.8
MS.gene48023.t1	MTR_8g041770	24.265	408	249	14	23	408	22	391	2.78e-13	72.0
MS.gene48023.t1	MTR_3g465980	23.894	339	212	11	49	365	50	364	1.89e-12	69.3
MS.gene48023.t1	MTR_3g026310	25.505	396	245	16	36	408	32	400	3.44e-12	68.6
MS.gene48023.t1	MTR_6g045553	23.371	445	263	15	8	407	12	423	3.55e-12	68.6
MS.gene48023.t1	MTR_0042s0190	23.938	259	161	9	154	410	1	225	5.03e-12	67.0
MS.gene48023.t1	MTR_4g022670	23.822	382	238	13	49	408	43	393	1.62e-11	66.6
MS.gene48023.t1	MTR_4g022290	22.906	406	231	15	36	408	38	394	1.88e-11	66.2
MS.gene48023.t1	MTR_4g022720	24.000	375	229	12	59	408	44	387	2.89e-11	65.9
MS.gene48023.t1	MTR_4g022825	24.648	426	263	18	6	408	2	392	6.00e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48023.t1	AT1G32910	59.110	472	155	13	8	465	11	458	1.76e-177	505
MS.gene48023.t1	AT1G78990	57.759	464	169	9	8	465	11	453	1.97e-177	505
MS.gene48023.t1	AT5G16410	51.579	475	184	14	8	465	34	479	5.40e-158	456
MS.gene48023.t1	AT1G31490	37.020	443	230	14	8	435	4	412	6.66e-77	247
MS.gene48023.t1	AT5G48930	25.764	458	290	17	10	450	3	427	1.08e-34	134
MS.gene48023.t1	AT5G41040	26.263	396	238	14	31	409	38	396	2.54e-29	119
MS.gene48023.t1	AT5G41040	26.263	396	238	14	31	409	54	412	3.82e-29	119
MS.gene48023.t1	AT2G19070	24.516	465	304	15	18	460	12	451	9.92e-28	115
MS.gene48023.t1	AT5G57840	26.552	467	271	18	10	446	3	427	2.85e-27	114
MS.gene48023.t1	AT5G17540	25.918	463	261	17	4	434	3	415	1.10e-26	112
MS.gene48023.t1	AT3G03480	25.647	464	261	19	8	431	17	436	1.53e-25	109
MS.gene48023.t1	AT3G48720	25.768	423	260	12	3	409	2	386	2.40e-25	108
MS.gene48023.t1	AT5G63560	26.366	421	246	13	8	408	5	381	1.05e-22	100
MS.gene48023.t1	AT1G03390	24.714	437	270	18	2	410	4	409	3.47e-20	93.2
MS.gene48023.t1	AT1G27620	22.693	401	258	14	66	450	58	422	1.96e-19	90.9
MS.gene48023.t1	AT3G50270	26.330	376	217	14	60	408	56	398	9.52e-19	89.0
MS.gene48023.t1	AT1G28680	26.836	354	214	16	69	408	65	387	2.68e-18	87.4
MS.gene48023.t1	AT2G39980	26.087	414	225	17	45	408	47	429	1.46e-17	85.5
MS.gene48023.t1	AT2G40230	22.705	414	258	13	59	450	52	425	3.83e-17	84.0
MS.gene48023.t1	AT3G50280	24.129	373	225	15	61	408	54	393	3.85e-17	84.0
MS.gene48023.t1	AT1G24430	23.294	425	247	17	10	408	5	376	5.66e-17	83.2
MS.gene48023.t1	AT3G30280	25.000	436	262	13	10	426	6	395	1.11e-16	82.4
MS.gene48023.t1	AT1G28680	26.688	311	193	13	69	365	65	354	3.42e-16	80.5
MS.gene48023.t1	AT5G47950	23.478	460	277	16	10	450	6	409	2.82e-15	78.2
MS.gene48023.t1	AT5G07860	21.655	411	251	12	27	408	30	398	4.75e-15	77.4
MS.gene48023.t1	AT5G01210	24.474	380	201	13	79	408	75	418	4.34e-14	74.7
MS.gene48023.t1	AT5G07080	23.514	370	232	11	60	408	64	403	5.01e-13	71.2
MS.gene48023.t1	AT2G25150	23.352	364	234	11	63	408	64	400	6.47e-13	70.9
MS.gene48023.t1	AT4G15390	24.171	422	259	17	10	409	8	390	7.26e-13	70.9
MS.gene48023.t1	AT4G15390	24.171	422	259	17	10	409	8	390	7.26e-13	70.9
MS.gene48023.t1	AT4G15400	24.601	439	264	16	7	424	2	394	8.14e-13	70.5
MS.gene48023.t1	AT3G50300	24.661	369	224	16	63	408	56	393	3.69e-12	68.6
MS.gene48023.t1	AT5G07850	23.562	365	225	13	66	408	70	402	4.12e-12	68.6
MS.gene48023.t1	AT1G24420	23.864	440	251	18	7	414	2	389	4.64e-12	68.2
MS.gene48023.t1	AT1G24420	23.864	440	251	18	7	414	2	389	4.64e-12	68.2
MS.gene48023.t1	AT5G67160	23.788	433	253	19	59	467	49	428	1.16e-11	67.0
MS.gene48023.t1	AT5G47980	22.300	426	262	13	7	407	2	383	4.37e-11	65.1

Find 128 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGATTATGAATTCATATTT+TGG	0.139298	5.2:-76864556	MS.gene48023:CDS
TTCTGCTTTCTTGTGGAAAA+TGG	0.174817	5.2:-76864680	MS.gene48023:CDS
CCAGAGTCGAAGGTTGATTT+TGG	0.189640	5.2:-76864292	MS.gene48023:CDS
TAGTATATGCGACTTATTAT+TGG	0.243557	5.2:+76864790	None:intergenic
AGTACACCATGTTTCTTCTT+TGG	0.264818	5.2:+76865384	None:intergenic
TTAATATCAGAGTCAGATTC+AGG	0.280399	5.2:+76864820	None:intergenic
GTTTCTTATTATGCCTTTGC+TGG	0.282844	5.2:-76865549	MS.gene48023:CDS
GGATGGAAGGTGGGCGTCGT+GGG	0.311586	5.2:+76864899	None:intergenic
AAGAAACATGTTTGCAGAAT+AGG	0.336073	5.2:+76865008	None:intergenic
TAAGGGACACTTGCACTTCA+TGG	0.343370	5.2:-76864152	MS.gene48023:CDS
CAAACATGTTTCTTGTATCA+TGG	0.346322	5.2:-76864999	MS.gene48023:CDS
GGTGACAGAGGAGCATTTCT+TGG	0.346724	5.2:-76864431	MS.gene48023:CDS
ATTTCTTGGGTTTAATAGAT+TGG	0.349608	5.2:-76864417	MS.gene48023:CDS
GAAGAGCATCGTCCAGTTCC+TGG	0.354253	5.2:-76864391	MS.gene48023:CDS
AGCAGCCACTATTTCTTTGT+TGG	0.356679	5.2:+76865734	None:intergenic
TGGATGGAAGGTGGGCGTCG+TGG	0.357861	5.2:+76864898	None:intergenic
TGCCAATGCCAAGCCCTAAA+AGG	0.358606	5.2:-76864207	MS.gene48023:CDS
AAGAACACACCAACATCAAC+TGG	0.358958	5.2:+76865654	None:intergenic
TTTCCTTGGGGAGGTAGTGC+TGG	0.358995	5.2:-76864238	MS.gene48023:CDS
AGTATATGCGACTTATTATT+GGG	0.359817	5.2:+76864791	None:intergenic
AAGAGATTGGTCTAAAGAAA+TGG	0.367262	5.2:+76864116	None:intergenic
TCAAACACCCCACAATGTTA+TGG	0.372360	5.2:+76865595	None:intergenic
TCAGAGTCAGATTCAGGTTC+AGG	0.387820	5.2:+76864826	None:intergenic
TTTCTTGGGTTTAATAGATT+GGG	0.393187	5.2:-76864416	MS.gene48023:CDS
GATCAAAGACTTGTTATCAT+TGG	0.395387	5.2:+76864960	None:intergenic
ATTTCTTTAGACCAATCTCT+TGG	0.398784	5.2:-76864114	MS.gene48023:CDS
CCAAAATCAACCTTCGACTC+TGG	0.403209	5.2:+76864292	None:intergenic
ATCAAAGACTTGTTATCATT+GGG	0.403427	5.2:+76864961	None:intergenic
TTCCCTTCAACAGATTCATC+TGG	0.408828	5.2:+76865414	None:intergenic
CGAAAACAAGGTTGAGTTGT+TGG	0.411658	5.2:+76864937	None:intergenic
AGAGATTGGTCTAAAGAAAT+GGG	0.420021	5.2:+76864117	None:intergenic
ATTAGGTCAAGGTTTGATAG+TGG	0.421854	5.2:+76865684	None:intergenic
CTAGTCTCCAAGATGTGTAT+TGG	0.425893	5.2:-76865783	None:intergenic
ACTACCATCATTGTTTAAGT+TGG	0.426749	5.2:+76864729	None:intergenic
ATCCTTGCCAATCCAGGAAC+TGG	0.426912	5.2:+76864379	None:intergenic
CCAACATTTGTGGTATCTTC+AGG	0.429306	5.2:-76864325	MS.gene48023:CDS
ATCATTGGGCCGAGCTATCT+CGG	0.444473	5.2:+76864975	None:intergenic
GCTTTACATGCACCTTCCTA+AGG	0.445432	5.2:-76864170	MS.gene48023:CDS
CATCATTGTTTAAGTTGGCT+AGG	0.450301	5.2:+76864734	None:intergenic
GTGACAGAGGAGCATTTCTT+GGG	0.453426	5.2:-76864430	MS.gene48023:CDS
ATGAAGTGCAAGTGTCCCTT+AGG	0.455932	5.2:+76864154	None:intergenic
GAAAACAAGGTTGAGTTGTT+GGG	0.463177	5.2:+76864938	None:intergenic
TTTCCACAAGAAAGCAGAAA+AGG	0.464789	5.2:+76864684	None:intergenic
GGTTGCTGAAGCCGCTTCAA+TGG	0.466106	5.2:-76864659	MS.gene48023:CDS
ATCATTGTTTAAGTTGGCTA+GGG	0.469219	5.2:+76864735	None:intergenic
CTCTGCCAATGCAAGAGCAC+TGG	0.470684	5.2:-76865710	MS.gene48023:CDS
TCCAAGATGTGTATTGGAAA+AGG	0.474951	5.2:-76865777	None:intergenic
TTTCTTTAGACCAATCTCTT+GGG	0.478661	5.2:-76864113	MS.gene48023:CDS
ATGAGGTAATCCCAAGAGAT+TGG	0.494084	5.2:+76864103	None:intergenic
CAATAAATCCTTGCCAATCC+AGG	0.495236	5.2:+76864373	None:intergenic
TATTACCAACAAAGAAATAG+TGG	0.495558	5.2:-76865739	MS.gene48023:CDS
TCAGATTCAGGTTCAGGTGG+TGG	0.495798	5.2:+76864832	None:intergenic
GGCACCCACCATAACATTGT+GGG	0.501925	5.2:-76865603	MS.gene48023:CDS
AAACATGTTTCTTGTATCAT+GGG	0.504377	5.2:-76864998	MS.gene48023:CDS
AGGATTTATTGTGGTAGCAC+TGG	0.506494	5.2:-76864361	MS.gene48023:CDS
GTCATAAATTGAAGGATGGA+TGG	0.511206	5.2:+76864882	None:intergenic
GGTTGATAATCCGCTATGTT+GGG	0.513429	5.2:-76864488	MS.gene48023:CDS
AATAAGCCTGTGATTACTAC+TGG	0.513795	5.2:-76865624	MS.gene48023:CDS
TTCTGCTAATCTACCACCAT+AGG	0.514784	5.2:+76864516	None:intergenic
AATCCAGATGAATCTGTTGA+AGG	0.515928	5.2:-76865417	MS.gene48023:CDS
GCATCGTCCAGTTCCTGGAT+TGG	0.519656	5.2:-76864386	MS.gene48023:CDS
TTGAAGGATGGATGGAAGGT+GGG	0.520996	5.2:+76864890	None:intergenic
ATCCAGATGAATCTGTTGAA+GGG	0.525156	5.2:-76865416	MS.gene48023:CDS
CTAGCCAACTTAAACAATGA+TGG	0.525446	5.2:-76864733	MS.gene48023:CDS
GGTAGCACTGGTGGAGAAGA+AGG	0.530503	5.2:-76864349	MS.gene48023:CDS
ATTGAAGGATGGATGGAAGG+TGG	0.539276	5.2:+76864889	None:intergenic
TATCATGGGCCGAGATAGCT+CGG	0.539461	5.2:-76864984	MS.gene48023:CDS
ATTTATTGTGGTAGCACTGG+TGG	0.539802	5.2:-76864358	MS.gene48023:CDS
TGGTTGATAATCCGCTATGT+TGG	0.541576	5.2:-76864489	MS.gene48023:CDS
AGAAGAAGGACCAACATTTG+TGG	0.543509	5.2:-76864335	MS.gene48023:CDS
TTGTTATTGTGCAGGCAACT+TGG	0.544767	5.2:-76865086	MS.gene48023:intron
GATTCAGGTTCAGGTGGTGG+AGG	0.546175	5.2:+76864835	None:intergenic
TATGCGACTTATTATTGGGT+CGG	0.548443	5.2:+76864795	None:intergenic
GATGGAAGGTGGGCGTCGTG+GGG	0.548673	5.2:+76864900	None:intergenic
AACACAAACTTTAATTGATG+AGG	0.548741	5.2:+76864086	None:intergenic
TTTGGAGGCGGCGGTGACAG+AGG	0.555868	5.2:-76864443	MS.gene48023:CDS
AGTCATCACTTCACCAGCAA+AGG	0.556336	5.2:+76865536	None:intergenic
AGAAGTGACCGTCGAAAACA+AGG	0.557353	5.2:+76864925	None:intergenic
GCCCTAAAAGGAATGGTGAC+TGG	0.557544	5.2:-76864195	MS.gene48023:CDS
GGAAGCCAGTGCTCTTGCAT+TGG	0.564426	5.2:+76865705	None:intergenic
CTTGGGTTTAATAGATTGGG+TGG	0.568431	5.2:-76864413	MS.gene48023:CDS
TGGTAGATTAGCAGAAGAGC+TGG	0.570353	5.2:-76864509	MS.gene48023:CDS
CGATTGTTGCAAAGTAACTC+AGG	0.570367	5.2:+76865498	None:intergenic
TAAATTGAAGGATGGATGGA+AGG	0.573429	5.2:+76864886	None:intergenic
AGAACACACCAACATCAACT+GGG	0.575989	5.2:+76865655	None:intergenic
CCTGAAGATACCACAAATGT+TGG	0.579526	5.2:+76864325	None:intergenic
CTTTACATGCACCTTCCTAA+GGG	0.579880	5.2:-76864169	MS.gene48023:CDS
AGAAACAAGAGCCTCACCTA+AGG	0.581520	5.2:+76865566	None:intergenic
ACAAAGGTTTCCAGAGTCGA+AGG	0.585762	5.2:-76864302	MS.gene48023:CDS
ACATACATGTCATAAATTGA+AGG	0.587593	5.2:+76864874	None:intergenic
GGTGGAGGTAGATCAGAGAC+GGG	0.587879	5.2:+76864850	None:intergenic
AACACCCCACAATGTTATGG+TGG	0.588442	5.2:+76865598	None:intergenic
TGGCACCCACCATAACATTG+TGG	0.591108	5.2:-76865604	MS.gene48023:CDS
TTGTGGTATCTTCAGGACAA+AGG	0.593564	5.2:-76864318	MS.gene48023:CDS
ACATGTCATAAATTGAAGGA+TGG	0.593580	5.2:+76864878	None:intergenic
ATGCCAAGCCCTAAAAGGAA+TGG	0.595010	5.2:-76864202	MS.gene48023:CDS
TCTGCTAATCTACCACCATA+GGG	0.595034	5.2:+76864517	None:intergenic
TGGTGGAGGTAGATCAGAGA+CGG	0.600538	5.2:+76864849	None:intergenic
AGTGCAAGTGTCCCTTAGGA+AGG	0.602305	5.2:+76864158	None:intergenic
TTGTGGTTGACGGAAGAAAG+AGG	0.605169	5.2:-76864606	MS.gene48023:CDS
GAGTTACTTTGCAACAATCG+TGG	0.605999	5.2:-76865495	MS.gene48023:CDS
GTCCAGTTCCTGGATTGGCA+AGG	0.607030	5.2:-76864381	MS.gene48023:CDS
AATGTGCTTTCGATTCCCTA+TGG	0.610089	5.2:-76864532	MS.gene48023:CDS
AATAGGCATCCGCTATGCGA+TGG	0.614428	5.2:+76865025	None:intergenic
AAGTAACTCAGGTTCACCCA+TGG	0.618644	5.2:+76865509	None:intergenic
GTGCTTTCGATTCCCTATGG+TGG	0.619185	5.2:-76864529	MS.gene48023:CDS
TGAAGTGATGACTAACTCCA+TGG	0.619717	5.2:-76865526	MS.gene48023:CDS
AAAATGGGGCTTGTGGTTGA+CGG	0.619932	5.2:-76864616	MS.gene48023:CDS
CTTGTTCCAAAGAAGAAACA+TGG	0.620389	5.2:-76865390	MS.gene48023:CDS
TGGGTGCCAGTAGTAATCAC+AGG	0.626482	5.2:+76865618	None:intergenic
ACACCCCACAATGTTATGGT+GGG	0.636435	5.2:+76865599	None:intergenic
GAAATGTGGTGGCTTAGTAG+TGG	0.648560	5.2:-76865061	MS.gene48023:CDS
GGATTGGCAAGGATTTATTG+TGG	0.648587	5.2:-76864370	MS.gene48023:CDS
GAGTCAGATTCAGGTTCAGG+TGG	0.651262	5.2:+76864829	None:intergenic
GCACCCACCATAACATTGTG+GGG	0.654145	5.2:-76865602	MS.gene48023:CDS
AAATGATAAGATCCAAACAC+AGG	0.657847	5.2:+76864259	None:intergenic
TGATAATCCGCTATGTTGGG+TGG	0.663374	5.2:-76864485	MS.gene48023:CDS
GAACACACCAACATCAACTG+GGG	0.669825	5.2:+76865656	None:intergenic
GAGATTGGTCTAAAGAAATG+GGG	0.678557	5.2:+76864118	None:intergenic
AACACACCAACATCAACTGG+GGG	0.688247	5.2:+76865657	None:intergenic
CAGGCAACTTGGATGAAATG+TGG	0.695087	5.2:-76865075	MS.gene48023:CDS
TAGCCAGCACTACCTCCCCA+AGG	0.698597	5.2:+76864235	None:intergenic
GAAGTGATGACTAACTCCAT+GGG	0.699827	5.2:-76865525	MS.gene48023:CDS
GCAACTTGGATGAAATGTGG+TGG	0.707819	5.2:-76865072	MS.gene48023:CDS
GGGACACTTGCACTTCATGG+AGG	0.714604	5.2:-76864149	MS.gene48023:CDS
CGATCTGCCACCCAACATAG+CGG	0.715687	5.2:+76864478	None:intergenic
GGAAGAAAGAGGCTAAGCAA+CGG	0.723389	5.2:-76864595	MS.gene48023:CDS
GTGGAGGTAGATCAGAGACG+GGG	0.788848	5.2:+76864851	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAACATAGAAATTTTTTTTT+GGG	-	chr5.2:76864579-76864598	MS.gene48023:CDS	10.0%
!!!	TAAACATAGAAATTTTTTTT+TGG	-	chr5.2:76864578-76864597	MS.gene48023:CDS	10.0%
!!	GTAAAATAGTATTAATTTCA+GGG	-	chr5.2:76864516-76864535	MS.gene48023:CDS	15.0%
!!!	AGATTATGAATTCATATTTT+GGG	-	chr5.2:76865312-76865331	MS.gene48023:intron	15.0%
!!!	ATAATACTAATTCTTTTGTA+AGG	+	chr5.2:76864743-76864762	None:intergenic	15.0%
!!!	TAATACTAATTCTTTTGTAA+GGG	+	chr5.2:76864742-76864761	None:intergenic	15.0%
!!	GGTAAAATAGTATTAATTTC+AGG	-	chr5.2:76864515-76864534	MS.gene48023:CDS	20.0%
!!!	GAGATTATGAATTCATATTT+TGG	-	chr5.2:76865311-76865330	MS.gene48023:intron	20.0%
!	AAAACATTGTTGCAAAAATG+GGG	-	chr5.2:76865237-76865256	MS.gene48023:intron	25.0%
!	AAACATGTTTCTTGTATCAT+GGG	-	chr5.2:76864869-76864888	MS.gene48023:CDS	25.0%
!	ACATACATGTCATAAATTGA+AGG	+	chr5.2:76864996-76865015	None:intergenic	25.0%
!	AGTATATGCGACTTATTATT+GGG	+	chr5.2:76865079-76865098	None:intergenic	25.0%
!	ATTTCTTGGGTTTAATAGAT+TGG	-	chr5.2:76865450-76865469	MS.gene48023:CDS	25.0%
!	GAAAACATTGTTGCAAAAAT+GGG	-	chr5.2:76865236-76865255	MS.gene48023:intron	25.0%
!	TAGTATATGCGACTTATTAT+TGG	+	chr5.2:76865080-76865099	None:intergenic	25.0%
!	TATTACCAACAAAGAAATAG+TGG	-	chr5.2:76864128-76864147	MS.gene48023:CDS	25.0%
!	TGAAAACATTGTTGCAAAAA+TGG	-	chr5.2:76865235-76865254	MS.gene48023:intron	25.0%
!	TTTCTTGGGTTTAATAGATT+GGG	-	chr5.2:76865451-76865470	MS.gene48023:CDS	25.0%
!!	AATTATCATTGTTATTGTGC+AGG	-	chr5.2:76864773-76864792	MS.gene48023:CDS	25.0%
!!!	ATCAAAGACTTGTTATCATT+GGG	+	chr5.2:76864909-76864928	None:intergenic	25.0%
	AAATGATAAGATCCAAACAC+AGG	+	chr5.2:76865611-76865630	None:intergenic	30.0%
	AAGAAACATGTTTGCAGAAT+AGG	+	chr5.2:76864862-76864881	None:intergenic	30.0%
	ACATGTCATAAATTGAAGGA+TGG	+	chr5.2:76864992-76865011	None:intergenic	30.0%
	ACTACCATCATTGTTTAAGT+TGG	+	chr5.2:76865141-76865160	None:intergenic	30.0%
	AGGCAAAGAATTATCATCAT+CGG	-	chr5.2:76864684-76864703	MS.gene48023:CDS	30.0%
	ATTTCTTTAGACCAATCTCT+TGG	-	chr5.2:76865753-76865772	MS.gene48023:CDS	30.0%
	CAAACATGTTTCTTGTATCA+TGG	-	chr5.2:76864868-76864887	MS.gene48023:CDS	30.0%
	TTAATATCAGAGTCAGATTC+AGG	+	chr5.2:76865050-76865069	None:intergenic	30.0%
	TTTCTTTAGACCAATCTCTT+GGG	-	chr5.2:76865754-76865773	MS.gene48023:CDS	30.0%
	TTTGAAAAATTCCTTAGGTG+AGG	-	chr5.2:76864290-76864309	MS.gene48023:CDS	30.0%
!	GTTTTCATTATCCATTGAAG+CGG	+	chr5.2:76865222-76865241	None:intergenic	30.0%
!	TAGATATCGACCAAACATTT+TGG	-	chr5.2:76864641-76864660	MS.gene48023:CDS	30.0%
!	TGGATCTTATCATTTTCCTT+GGG	-	chr5.2:76865616-76865635	MS.gene48023:CDS	30.0%
!	TTGGATCTTATCATTTTCCT+TGG	-	chr5.2:76865615-76865634	MS.gene48023:CDS	30.0%
!!	AAGAGATTGGTCTAAAGAAA+TGG	+	chr5.2:76865754-76865773	None:intergenic	30.0%
!!	AGAGATTGGTCTAAAGAAAT+GGG	+	chr5.2:76865753-76865772	None:intergenic	30.0%
!!	ATCATTGTTTAAGTTGGCTA+GGG	+	chr5.2:76865135-76865154	None:intergenic	30.0%
!!	GATCAAAGACTTGTTATCAT+TGG	+	chr5.2:76864910-76864929	None:intergenic	30.0%
	AATAAGCCTGTGATTACTAC+TGG	-	chr5.2:76864243-76864262	MS.gene48023:CDS	35.0%
	AATCCAGATGAATCTGTTGA+AGG	-	chr5.2:76864450-76864469	MS.gene48023:CDS	35.0%
	AGTACACCATGTTTCTTCTT+TGG	+	chr5.2:76864486-76864505	None:intergenic	35.0%
	ATCCAGATGAATCTGTTGAA+GGG	-	chr5.2:76864451-76864470	MS.gene48023:CDS	35.0%
	ATTAGGTCAAGGTTTGATAG+TGG	+	chr5.2:76864186-76864205	None:intergenic	35.0%
	CTAGCCAACTTAAACAATGA+TGG	-	chr5.2:76865134-76865153	MS.gene48023:intron	35.0%
	CTTGTTCCAAAGAAGAAACA+TGG	-	chr5.2:76864477-76864496	MS.gene48023:CDS	35.0%
	GGGTGTTTGAAAAATTCCTT+AGG	-	chr5.2:76864285-76864304	MS.gene48023:CDS	35.0%
	GTCATAAATTGAAGGATGGA+TGG	+	chr5.2:76864988-76865007	None:intergenic	35.0%
	GTTTCTTATTATGCCTTTGC+TGG	-	chr5.2:76864318-76864337	MS.gene48023:CDS	35.0%
	TAAATTGAAGGATGGATGGA+AGG	+	chr5.2:76864984-76865003	None:intergenic	35.0%
	TATGCGACTTATTATTGGGT+CGG	+	chr5.2:76865075-76865094	None:intergenic	35.0%
	TCTCGAGGGAAAATTAGAAT+AGG	-	chr5.2:76864664-76864683	MS.gene48023:CDS	35.0%
	TTCTGCTTTCTTGTGGAAAA+TGG	-	chr5.2:76865187-76865206	MS.gene48023:intron	35.0%
	TTTCCACAAGAAAGCAGAAA+AGG	+	chr5.2:76865186-76865205	None:intergenic	35.0%
!	AGATCGAGTTCATGAGTTTT+TGG	-	chr5.2:76865406-76865425	MS.gene48023:CDS	35.0%
!	AGTTTTTAAACTTGTGCCCT+TGG	+	chr5.2:76864559-76864578	None:intergenic	35.0%
!	GAAAACAAGGTTGAGTTGTT+GGG	+	chr5.2:76864932-76864951	None:intergenic	35.0%
!	GGATCTTATCATTTTCCTTG+GGG	-	chr5.2:76865617-76865636	MS.gene48023:CDS	35.0%
!	GTTTTTAAACTTGTGCCCTT+GGG	+	chr5.2:76864558-76864577	None:intergenic	35.0%
!	TTTTTAAACTTGTGCCCTTG+GGG	+	chr5.2:76864557-76864576	None:intergenic	35.0%
!!	CATCATTGTTTAAGTTGGCT+AGG	+	chr5.2:76865136-76865155	None:intergenic	35.0%
!!	GAGATTGGTCTAAAGAAATG+GGG	+	chr5.2:76865752-76865771	None:intergenic	35.0%
	AAGAACACACCAACATCAAC+TGG	+	chr5.2:76864216-76864235	None:intergenic	40.0%
	AATGTGCTTTCGATTCCCTA+TGG	-	chr5.2:76865335-76865354	MS.gene48023:intron	40.0%
	AGAACACACCAACATCAACT+GGG	+	chr5.2:76864215-76864234	None:intergenic	40.0%
	AGAAGAAGGACCAACATTTG+TGG	-	chr5.2:76865532-76865551	MS.gene48023:CDS	40.0%
	AGGATTTATTGTGGTAGCAC+TGG	-	chr5.2:76865506-76865525	MS.gene48023:CDS	40.0%
	ATGAGGTAATCCCAAGAGAT+TGG	+	chr5.2:76865767-76865786	None:intergenic	40.0%
	CAATAAATCCTTGCCAATCC+AGG	+	chr5.2:76865497-76865516	None:intergenic	40.0%
	CCAACATTTGTGGTATCTTC+AGG	-	chr5.2:76865542-76865561	MS.gene48023:CDS	40.0%
	CCTGAAGATACCACAAATGT+TGG	+	chr5.2:76865545-76865564	None:intergenic	40.0%
	CGATTGTTGCAAAGTAACTC+AGG	+	chr5.2:76864372-76864391	None:intergenic	40.0%
	CTTGGGTTTAATAGATTGGG+TGG	-	chr5.2:76865454-76865473	MS.gene48023:CDS	40.0%
	CTTTACATGCACCTTCCTAA+GGG	-	chr5.2:76865698-76865717	MS.gene48023:CDS	40.0%
	GAAGTGATGACTAACTCCAT+GGG	-	chr5.2:76864342-76864361	MS.gene48023:CDS	40.0%
	TCAAACACCCCACAATGTTA+TGG	+	chr5.2:76864275-76864294	None:intergenic	40.0%
	TCTGCTAATCTACCACCATA+GGG	+	chr5.2:76865353-76865372	None:intergenic	40.0%
	TGAAGTGATGACTAACTCCA+TGG	-	chr5.2:76864341-76864360	MS.gene48023:CDS	40.0%
	TTCCCTTCAACAGATTCATC+TGG	+	chr5.2:76864456-76864475	None:intergenic	40.0%
	TTCTGCTAATCTACCACCAT+AGG	+	chr5.2:76865354-76865373	None:intergenic	40.0%
	TTGTGGTATCTTCAGGACAA+AGG	-	chr5.2:76865549-76865568	MS.gene48023:CDS	40.0%
!	AACCAGTCACCATTCCTTTT+AGG	+	chr5.2:76865677-76865696	None:intergenic	40.0%
!	ACCAGTCACCATTCCTTTTA+GGG	+	chr5.2:76865676-76865695	None:intergenic	40.0%
!	AGCAGCCACTATTTCTTTGT+TGG	+	chr5.2:76864136-76864155	None:intergenic	40.0%
!	AGTCCTTTTCTGCTTTCTTG+TGG	-	chr5.2:76865180-76865199	MS.gene48023:intron	40.0%
!	ATTTATTGTGGTAGCACTGG+TGG	-	chr5.2:76865509-76865528	MS.gene48023:CDS	40.0%
!	CGAAAACAAGGTTGAGTTGT+TGG	+	chr5.2:76864933-76864952	None:intergenic	40.0%
!	GAGTTACTTTGCAACAATCG+TGG	-	chr5.2:76864372-76864391	MS.gene48023:CDS	40.0%
!	GGATTGGCAAGGATTTATTG+TGG	-	chr5.2:76865497-76865516	MS.gene48023:CDS	40.0%
!	TCTTATCATTTTCCTTGGGG+AGG	-	chr5.2:76865620-76865639	MS.gene48023:CDS	40.0%
!	TTGTTATTGTGCAGGCAACT+TGG	-	chr5.2:76864781-76864800	MS.gene48023:CDS	40.0%
!!	AAATGTTAACTTGTGCCCCA+AGG	-	chr5.2:76864539-76864558	MS.gene48023:CDS	40.0%
!!	AATGTTAACTTGTGCCCCAA+GGG	-	chr5.2:76864540-76864559	MS.gene48023:CDS	40.0%
!!	ACCAAACATTTTGGTCTCGA+GGG	-	chr5.2:76864650-76864669	MS.gene48023:CDS	40.0%
!!	AGTCGAAGGTTGATTTTGGA+TGG	-	chr5.2:76865579-76865598	MS.gene48023:CDS	40.0%
!!	AGTTCATGAGTTTTTGGAGG+CGG	-	chr5.2:76865412-76865431	MS.gene48023:CDS	40.0%
!!	GGTTGATAATCCGCTATGTT+GGG	-	chr5.2:76865379-76865398	MS.gene48023:intron	40.0%
!!	GTCGAAGGTTGATTTTGGAT+GGG	-	chr5.2:76865580-76865599	MS.gene48023:CDS	40.0%
!!	TCGAAGGTTGATTTTGGATG+GGG	-	chr5.2:76865581-76865600	MS.gene48023:CDS	40.0%
!!	TCGAGTTCATGAGTTTTTGG+AGG	-	chr5.2:76865409-76865428	MS.gene48023:CDS	40.0%
!!	TGGTTGATAATCCGCTATGT+TGG	-	chr5.2:76865378-76865397	MS.gene48023:intron	40.0%
!!!	CAACTCAACCTTGTTTTCGA+CGG	-	chr5.2:76864934-76864953	MS.gene48023:CDS	40.0%
	AAAATGGGGCTTGTGGTTGA+CGG	-	chr5.2:76865251-76865270	MS.gene48023:intron	45.0%
	AACACACCAACATCAACTGG+GGG	+	chr5.2:76864213-76864232	None:intergenic	45.0%
	AACACCCCACAATGTTATGG+TGG	+	chr5.2:76864272-76864291	None:intergenic	45.0%
	AAGTAACTCAGGTTCACCCA+TGG	+	chr5.2:76864361-76864380	None:intergenic	45.0%
	ACAAAGGTTTCCAGAGTCGA+AGG	-	chr5.2:76865565-76865584	MS.gene48023:CDS	45.0%
	ACACCCCACAATGTTATGGT+GGG	+	chr5.2:76864271-76864290	None:intergenic	45.0%
	AGAAACAAGAGCCTCACCTA+AGG	+	chr5.2:76864304-76864323	None:intergenic	45.0%
	AGAAGTGACCGTCGAAAACA+AGG	+	chr5.2:76864945-76864964	None:intergenic	45.0%
	AGTCATCACTTCACCAGCAA+AGG	+	chr5.2:76864334-76864353	None:intergenic	45.0%
	ATGAAGTGCAAGTGTCCCTT+AGG	+	chr5.2:76865716-76865735	None:intergenic	45.0%
	ATGCCAAGCCCTAAAAGGAA+TGG	-	chr5.2:76865665-76865684	MS.gene48023:CDS	45.0%
	ATTGAAGGATGGATGGAAGG+TGG	+	chr5.2:76864981-76865000	None:intergenic	45.0%
	CAGGCAACTTGGATGAAATG+TGG	-	chr5.2:76864792-76864811	MS.gene48023:CDS	45.0%
	CATCAACTGGGGGAAGAATT+AGG	+	chr5.2:76864203-76864222	None:intergenic	45.0%
	CCAAAATCAACCTTCGACTC+TGG	+	chr5.2:76865578-76865597	None:intergenic	45.0%
	GAAATGTGGTGGCTTAGTAG+TGG	-	chr5.2:76864806-76864825	MS.gene48023:CDS	45.0%
	GAACACACCAACATCAACTG+GGG	+	chr5.2:76864214-76864233	None:intergenic	45.0%
	GCAACTTGGATGAAATGTGG+TGG	-	chr5.2:76864795-76864814	MS.gene48023:CDS	45.0%
	GCTTTACATGCACCTTCCTA+AGG	-	chr5.2:76865697-76865716	MS.gene48023:CDS	45.0%
	GGAAGAAAGAGGCTAAGCAA+CGG	-	chr5.2:76865272-76865291	MS.gene48023:intron	45.0%
	TAAGGGACACTTGCACTTCA+TGG	-	chr5.2:76865715-76865734	MS.gene48023:CDS	45.0%
	TCCCTCGAGACCAAAATGTT+TGG	+	chr5.2:76864654-76864673	None:intergenic	45.0%
	TGATAATCCGCTATGTTGGG+TGG	-	chr5.2:76865382-76865401	MS.gene48023:intron	45.0%
	TGGTAGATTAGCAGAAGAGC+TGG	-	chr5.2:76865358-76865377	MS.gene48023:intron	45.0%
	TGTTGCAAAAATGGGGCTTG+TGG	-	chr5.2:76865244-76865263	MS.gene48023:intron	45.0%
	TTGAAGGATGGATGGAAGGT+GGG	+	chr5.2:76864980-76864999	None:intergenic	45.0%
	TTGTGGTTGACGGAAGAAAG+AGG	-	chr5.2:76865261-76865280	MS.gene48023:intron	45.0%
!	ATTCTTCCCCCAGTTGATGT+TGG	-	chr5.2:76864204-76864223	MS.gene48023:CDS	45.0%
!	CACTTTTGACCATCGCATAG+CGG	-	chr5.2:76864833-76864852	MS.gene48023:CDS	45.0%
!	TCAGAGTCAGATTCAGGTTC+AGG	+	chr5.2:76865044-76865063	None:intergenic	45.0%
!!	ACATGGTGTACTTGCTGTTC+AGG	-	chr5.2:76864494-76864513	MS.gene48023:CDS	45.0%
!!	CCAGAGTCGAAGGTTGATTT+TGG	-	chr5.2:76865575-76865594	MS.gene48023:CDS	45.0%
!!	CGAAGGTTGATTTTGGATGG+GGG	-	chr5.2:76865582-76865601	MS.gene48023:CDS	45.0%
!!	GACCAAACATTTTGGTCTCG+AGG	-	chr5.2:76864649-76864668	MS.gene48023:CDS	45.0%
!!	GTGACAGAGGAGCATTTCTT+GGG	-	chr5.2:76865437-76865456	MS.gene48023:CDS	45.0%
!!!	TCACCATTCCTTTTAGGGCT+TGG	+	chr5.2:76865671-76865690	None:intergenic	45.0%
!!!	TTCCTTTTAGGGCTTGGCAT+TGG	+	chr5.2:76865665-76865684	None:intergenic	45.0%
	AATAGGCATCCGCTATGCGA+TGG	+	chr5.2:76864845-76864864	None:intergenic	50.0%
	AGTGCAAGTGTCCCTTAGGA+AGG	+	chr5.2:76865712-76865731	None:intergenic	50.0%
	ATCATTGGGCCGAGCTATCT+CGG	+	chr5.2:76864895-76864914	None:intergenic	50.0%
	ATCCTTGCCAATCCAGGAAC+TGG	+	chr5.2:76865491-76865510	None:intergenic	50.0%
	CTGGGGGAAGAATTAGGTCA+AGG	+	chr5.2:76864197-76864216	None:intergenic	50.0%
	GCACCCACCATAACATTGTG+GGG	-	chr5.2:76864265-76864284	MS.gene48023:CDS	50.0%
	GCCCTAAAAGGAATGGTGAC+TGG	-	chr5.2:76865672-76865691	MS.gene48023:CDS	50.0%
	GGCACCCACCATAACATTGT+GGG	-	chr5.2:76864264-76864283	MS.gene48023:CDS	50.0%
	GTGCTTTCGATTCCCTATGG+TGG	-	chr5.2:76865338-76865357	MS.gene48023:intron	50.0%
	TATCATGGGCCGAGATAGCT+CGG	-	chr5.2:76864883-76864902	MS.gene48023:CDS	50.0%
	TGCCAATGCCAAGCCCTAAA+AGG	-	chr5.2:76865660-76865679	MS.gene48023:CDS	50.0%
	TGGCACCCACCATAACATTG+TGG	-	chr5.2:76864263-76864282	MS.gene48023:CDS	50.0%
	TGGGTGCCAGTAGTAATCAC+AGG	+	chr5.2:76864252-76864271	None:intergenic	50.0%
	TGGTGGAGGTAGATCAGAGA+CGG	+	chr5.2:76865021-76865040	None:intergenic	50.0%
!	GAGTCAGATTCAGGTTCAGG+TGG	+	chr5.2:76865041-76865060	None:intergenic	50.0%
!	TCAGATTCAGGTTCAGGTGG+TGG	+	chr5.2:76865038-76865057	None:intergenic	50.0%
!!	GGTGACAGAGGAGCATTTCT+TGG	-	chr5.2:76865436-76865455	MS.gene48023:CDS	50.0%
!!	TCATGAGTTTTTGGAGGCGG+CGG	-	chr5.2:76865415-76865434	MS.gene48023:CDS	50.0%
	CGATCTGCCACCCAACATAG+CGG	+	chr5.2:76865392-76865411	None:intergenic	55.0%
	GCATCGTCCAGTTCCTGGAT+TGG	-	chr5.2:76865481-76865500	MS.gene48023:CDS	55.0%
	GGAAGCCAGTGCTCTTGCAT+TGG	+	chr5.2:76864165-76864184	None:intergenic	55.0%
	GGGACACTTGCACTTCATGG+AGG	-	chr5.2:76865718-76865737	MS.gene48023:CDS	55.0%
	GGTGGAGGTAGATCAGAGAC+GGG	+	chr5.2:76865020-76865039	None:intergenic	55.0%
	GGTTGCTGAAGCCGCTTCAA+TGG	-	chr5.2:76865208-76865227	MS.gene48023:intron	55.0%
	GTGGAGGTAGATCAGAGACG+GGG	+	chr5.2:76865019-76865038	None:intergenic	55.0%
	TTTCCTTGGGGAGGTAGTGC+TGG	-	chr5.2:76865629-76865648	MS.gene48023:CDS	55.0%
!	CTCTGCCAATGCAAGAGCAC+TGG	-	chr5.2:76864157-76864176	MS.gene48023:CDS	55.0%
!	GATTCAGGTTCAGGTGGTGG+AGG	+	chr5.2:76865035-76865054	None:intergenic	55.0%
!!	GAAGAGCATCGTCCAGTTCC+TGG	-	chr5.2:76865476-76865495	MS.gene48023:CDS	55.0%
!!	GGTAGCACTGGTGGAGAAGA+AGG	-	chr5.2:76865518-76865537	MS.gene48023:CDS	55.0%
!!	GTCCAGTTCCTGGATTGGCA+AGG	-	chr5.2:76865486-76865505	MS.gene48023:CDS	55.0%
!!!	GGACTCGAGTTTTGAGCGCT+TGG	+	chr5.2:76865165-76865184	None:intergenic	55.0%
	GGATGGGGGAAGCCTGTGTT+TGG	-	chr5.2:76865596-76865615	MS.gene48023:CDS	60.0%
	TAGCCAGCACTACCTCCCCA+AGG	+	chr5.2:76865635-76865654	None:intergenic	60.0%
	TTTGGAGGCGGCGGTGACAG+AGG	-	chr5.2:76865424-76865443	MS.gene48023:CDS	65.0%
!	GATGGAAGGTGGGCGTCGTG+GGG	+	chr5.2:76864970-76864989	None:intergenic	65.0%
!	GGATGGAAGGTGGGCGTCGT+GGG	+	chr5.2:76864971-76864990	None:intergenic	65.0%
!	TGGATGGAAGGTGGGCGTCG+TGG	+	chr5.2:76864972-76864991	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	76864096	76865793	76864096	ID=MS.gene48023
chr5.2	mRNA	76864096	76865793	76864096	ID=MS.gene48023.t1;Parent=MS.gene48023
chr5.2	exon	76865389	76865793	76865389	ID=MS.gene48023.t1.exon1;Parent=MS.gene48023.t1
chr5.2	CDS	76865389	76865793	76865389	ID=cds.MS.gene48023.t1;Parent=MS.gene48023.t1
chr5.2	exon	76864096	76865094	76864096	ID=MS.gene48023.t1.exon2;Parent=MS.gene48023.t1
chr5.2	CDS	76864096	76865094	76864096	ID=cds.MS.gene48023.t1;Parent=MS.gene48023.t1

Gene Sequence

>MS.gene48023

ATGTGTATTGGAAAAGGAGAATTCACAGTGAATATTACCAACAAAGAAATAGTGGCTGCTGCTCTGCCAATGCAAGAGCACTGGCTTCCACTATCAAACCTTGACCTAATTCTTCCCCCAGTTGATGTTGGTGTGTTCTTTTGCTACAATAAGCCTGTGATTACTACTGGCACCCACCATAACATTGTGGGGTGTTTGAAAAATTCCTTAGGTGAGGCTCTTGTTTCTTATTATGCCTTTGCTGGTGAAGTGATGACTAACTCCATGGGTGAACCTGAGTTACTTTGCAACAATCGTGGAGTTGATTTTGTTGAAGCTTTTGCTGATGTTGAGTTACAATCTCTCAATTTGTATAATCCAGATGAATCTGTTGAAGGGAAGCTTGTTCCAAAGAAGAAACATGGTGTACTTGCTGTTCAGGTAAAATAGTATTAATTTCAGGGAAATGTTAACTTGTGCCCCAAGGGCACAAGTTTAAAAACTAAACATAGAAATTTTTTTTTGGGAACTGTACATTCATTGTATTAAAAGTTTAAAATTTTTAATAGATATCGACCAAACATTTTGGTCTCGAGGGAAAATTAGAATAGGCAAAGAATTATCATCATCGGCATGTAGTTTTTTTAATAATCATTAGTATTATCCCTTACAAAAGAATTAGTATTATAAAGTGTTTTAATTATCATTGTTATTGTGCAGGCAACTTGGATGAAATGTGGTGGCTTAGTAGTGGCTTGCACTTTTGACCATCGCATAGCGGATGCCTATTCTGCAAACATGTTTCTTGTATCATGGGCCGAGATAGCTCGGCCCAATGATAACAAGTCTTTGATCCCAACAACTCAACCTTGTTTTCGACGGTCACTTCTGACCCCACGACGCCCACCTTCCATCCATCCTTCAATTTATGACATGTATGTCCCCGTCTCTGATCTACCTCCACCACCTGAACCTGAATCTGACTCTGATATTAAAACCGACCCAATAATAAGTCGCATATACTATGTAACAAGCAAAGAACTCAACAACATGCAGTCCCTAGCCAACTTAAACAATGATGGTAGTTCCAAGCGCTCAAAACTCGAGTCCTTTTCTGCTTTCTTGTGGAAAATGGTTGCTGAAGCCGCTTCAATGGATAATGAAAACATTGTTGCAAAAATGGGGCTTGTGGTTGACGGAAGAAAGAGGCTAAGCAACGGTGACAAAAACAAAGAAGAGATTATGAATTCATATTTTGGGAATGTGCTTTCGATTCCCTATGGTGGTAGATTAGCAGAAGAGCTGGTTGATAATCCGCTATGTTGGGTGGCAGATCGAGTTCATGAGTTTTTGGAGGCGGCGGTGACAGAGGAGCATTTCTTGGGTTTAATAGATTGGGTGGAAGAGCATCGTCCAGTTCCTGGATTGGCAAGGATTTATTGTGGTAGCACTGGTGGAGAAGAAGGACCAACATTTGTGGTATCTTCAGGACAAAGGTTTCCAGAGTCGAAGGTTGATTTTGGATGGGGGAAGCCTGTGTTTGGATCTTATCATTTTCCTTGGGGAGGTAGTGCTGGCTATGTTATGCCAATGCCAAGCCCTAAAAGGAATGGTGACTGGTTGCTTTACATGCACCTTCCTAAGGGACACTTGCACTTCATGGAGGCTCAAGCTCCCCATTTCTTTAGACCAATCTCTTGGGATTACCTCATCAATTAA

Protein sequence

>MS.gene48023.t1

MCIGKGEFTVNITNKEIVAAALPMQEHWLPLSNLDLILPPVDVGVFFCYNKPVITTGTHHNIVGCLKNSLGEALVSYYAFAGEVMTNSMGEPELLCNNRGVDFVEAFADVELQSLNLYNPDESVEGKLVPKKKHGATWMKCGGLVVACTFDHRIADAYSANMFLVSWAEIARPNDNKSLIPTTQPCFRRSLLTPRRPPSIHPSIYDMYVPVSDLPPPPEPESDSDIKTDPIISRIYYVTSKELNNMQSLANLNNDGSSKRSKLESFSAFLWKMVAEAASMDNENIVAKMGLVVDGRKRLSNGDKNKEEIMNSYFGNVLSIPYGGRLAEELVDNPLCWVADRVHEFLEAAVTEEHFLGLIDWVEEHRPVPGLARIYCGSTGGEEGPTFVVSSGQRFPESKVDFGWGKPVFGSYHFPWGGSAGYVMPMPSPKRNGDWLLYMHLPKGHLHFMEAQAPHFFRPISWDYLIN