AlfalfaGEDB | MS.gene47719

M. sativa cultivar XinJiangDaYe / MS.gene47719

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47719.t1	XP_003617368.1	94.3	473	22	3	1	470	1	471	4.50E-265	923.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47719.t1	Q9FI78	25.4	457	296	16	10	453	3	427	1.2e-29	132.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47719.t1	G7K3A2	94.3	473	22	3	1	470	1	471	3.2e-265	923.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04926	MS.gene47719	0.810828	9.49E-51	-1.69E-46
MS.gene049549	MS.gene47719	0.842168	3.05E-58	-1.69E-46
MS.gene049813	MS.gene47719	0.806335	8.65E-50	-1.69E-46
MS.gene050329	MS.gene47719	0.80569	1.18E-49	-1.69E-46
MS.gene050479	MS.gene47719	0.814052	1.87E-51	-1.69E-46
MS.gene050547	MS.gene47719	0.811161	8.04E-51	-1.69E-46
MS.gene050647	MS.gene47719	0.819701	1.01E-52	-1.69E-46
MS.gene051127	MS.gene47719	0.801704	7.94E-49	-1.69E-46
MS.gene051440	MS.gene47719	0.806558	7.76E-50	-1.69E-46
MS.gene052211	MS.gene47719	0.85667	2.75E-62	-1.69E-46
MS.gene052212	MS.gene47719	0.815253	1.02E-51	-1.69E-46
MS.gene05240	MS.gene47719	0.852696	3.90E-61	-1.69E-46
MS.gene052606	MS.gene47719	0.809141	2.19E-50	-1.69E-46
MS.gene053031	MS.gene47719	0.803934	2.75E-49	-1.69E-46
MS.gene053403	MS.gene47719	0.800828	1.20E-48	-1.69E-46
MS.gene053406	MS.gene47719	0.858456	8.14E-63	-1.69E-46
MS.gene053887	MS.gene47719	0.810504	1.12E-50	-1.69E-46
MS.gene054146	MS.gene47719	0.855701	5.29E-62	-1.69E-46
MS.gene054147	MS.gene47719	0.827788	1.29E-54	-1.69E-46
MS.gene054277	MS.gene47719	0.846544	2.03E-59	-1.69E-46
MS.gene054742	MS.gene47719	0.862108	6.40E-64	-1.69E-46
MS.gene055124	MS.gene47719	0.808511	2.99E-50	-1.69E-46
MS.gene055139	MS.gene47719	0.802621	5.14E-49	-1.69E-46
MS.gene055240	MS.gene47719	0.841053	6.00E-58	-1.69E-46
MS.gene055340	MS.gene47719	0.80268	5.00E-49	-1.69E-46
MS.gene05589	MS.gene47719	-0.806338	8.64E-50	-1.69E-46
MS.gene056076	MS.gene47719	0.811128	8.17E-51	-1.69E-46
MS.gene056218	MS.gene47719	0.813404	2.60E-51	-1.69E-46
MS.gene056590	MS.gene47719	0.809603	1.74E-50	-1.69E-46
MS.gene056591	MS.gene47719	0.80726	5.51E-50	-1.69E-46
MS.gene056680	MS.gene47719	0.818958	1.49E-52	-1.69E-46
MS.gene056748	MS.gene47719	0.807556	4.77E-50	-1.69E-46
MS.gene056920	MS.gene47719	0.846615	1.94E-59	-1.69E-46
MS.gene056922	MS.gene47719	0.80743	5.07E-50	-1.69E-46
MS.gene057261	MS.gene47719	0.806144	9.49E-50	-1.69E-46
MS.gene057550	MS.gene47719	0.81021	1.29E-50	-1.69E-46
MS.gene057770	MS.gene47719	0.800821	1.20E-48	-1.69E-46
MS.gene058177	MS.gene47719	0.809156	2.18E-50	-1.69E-46
MS.gene05837	MS.gene47719	-0.802797	4.73E-49	-1.69E-46
MS.gene058381	MS.gene47719	0.821399	4.12E-53	-1.69E-46
MS.gene058603	MS.gene47719	0.824501	7.80E-54	-1.69E-46
MS.gene059060	MS.gene47719	0.800425	1.45E-48	-1.69E-46
MS.gene059955	MS.gene47719	0.805777	1.13E-49	-1.69E-46
MS.gene060087	MS.gene47719	0.853899	1.76E-61	-1.69E-46
MS.gene060358	MS.gene47719	0.809514	1.82E-50	-1.69E-46
MS.gene060593	MS.gene47719	0.804481	2.12E-49	-1.69E-46
MS.gene061038	MS.gene47719	0.814266	1.68E-51	-1.69E-46
MS.gene061092	MS.gene47719	0.837897	3.96E-57	-1.69E-46
MS.gene061094	MS.gene47719	0.837329	5.54E-57	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene012960	MS.gene47719	PPI
MS.gene012965	MS.gene47719	PPI
MS.gene012957	MS.gene47719	PPI
MS.gene038274	MS.gene47719	PPI
MS.gene47721	MS.gene47719	PPI
MS.gene38701	MS.gene47719	PPI
MS.gene38359	MS.gene47719	PPI
MS.gene32933	MS.gene47719	PPI
MS.gene038271	MS.gene47719	PPI
MS.gene38365	MS.gene47719	PPI
MS.gene028134	MS.gene47719	PPI
MS.gene95242	MS.gene47719	PPI
MS.gene32936	MS.gene47719	PPI
MS.gene019178	MS.gene47719	PPI
MS.gene017054	MS.gene47719	PPI
MS.gene017058	MS.gene47719	PPI
MS.gene32938	MS.gene47719	PPI
MS.gene38362	MS.gene47719	PPI
MS.gene38699	MS.gene47719	PPI
MS.gene000507	MS.gene47719	PPI
MS.gene69496	MS.gene47719	PPI
MS.gene47716	MS.gene47719	PPI
MS.gene38703	MS.gene47719	PPI
MS.gene22199	MS.gene47719	PPI
MS.gene38702	MS.gene47719	PPI
MS.gene38363	MS.gene47719	PPI
MS.gene039597	MS.gene47719	PPI
MS.gene72785	MS.gene47719	PPI
MS.gene48027	MS.gene47719	PPI
MS.gene83461	MS.gene47719	PPI
MS.gene47719	MS.gene82145	PPI
MS.gene48021	MS.gene47719	PPI
MS.gene48023	MS.gene47719	PPI
MS.gene017053	MS.gene47719	PPI
MS.gene38704	MS.gene47719	PPI
MS.gene069321	MS.gene47719	PPI
MS.gene38361	MS.gene47719	PPI
MS.gene38706	MS.gene47719	PPI
MS.gene32934	MS.gene47719	PPI
MS.gene012961	MS.gene47719	PPI
MS.gene046246	MS.gene47719	PPI
MS.gene012959	MS.gene47719	PPI
MS.gene38700	MS.gene47719	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47719.t1	MTR_5g090830	95.772	473	15	3	1	470	1	471	0.0	936
MS.gene47719.t1	MTR_5g090810	93.446	473	26	3	1	470	1	471	0.0	920
MS.gene47719.t1	MTR_5g090880	80.550	473	89	2	1	470	1	473	0.0	779
MS.gene47719.t1	MTR_3g450710	66.667	480	136	9	1	468	1	468	0.0	625
MS.gene47719.t1	MTR_3g450690	63.750	480	154	7	1	468	1	472	0.0	608
MS.gene47719.t1	MTR_5g026320	34.703	438	256	13	7	438	15	428	3.63e-69	228
MS.gene47719.t1	MTR_8g075610	25.747	435	274	15	32	453	16	414	1.14e-31	126
MS.gene47719.t1	MTR_8g096530	28.507	442	257	20	31	456	28	426	3.13e-31	125
MS.gene47719.t1	MTR_2g064495	25.813	461	288	19	10	453	3	426	3.86e-31	124
MS.gene47719.t1	MTR_2g105330	25.813	461	288	19	10	453	3	426	1.94e-30	122
MS.gene47719.t1	MTR_4g007540	24.819	415	271	13	10	412	3	388	1.81e-29	120
MS.gene47719.t1	MTR_4g097090	25.647	464	296	15	23	463	19	456	2.16e-29	120
MS.gene47719.t1	MTR_3g049150	26.637	443	262	18	31	444	23	431	1.53e-28	117
MS.gene47719.t1	MTR_4g127650	23.871	465	302	13	23	463	20	456	4.00e-28	116
MS.gene47719.t1	MTR_7g114470	26.303	422	259	15	8	413	9	394	2.99e-27	114
MS.gene47719.t1	MTR_3g098980	27.041	392	238	15	31	411	35	389	4.77e-27	113
MS.gene47719.t1	MTR_1g040215	26.434	401	232	14	63	434	61	427	2.15e-26	111
MS.gene47719.t1	MTR_7g027175	26.718	393	241	14	31	412	37	393	1.16e-25	109
MS.gene47719.t1	MTR_7g114560	24.295	461	284	14	8	445	8	426	2.75e-25	108
MS.gene47719.t1	MTR_5g076700	24.835	455	290	16	8	445	8	427	2.96e-25	108
MS.gene47719.t1	MTR_7g086000	28.150	373	216	15	66	410	60	408	1.20e-24	106
MS.gene47719.t1	MTR_7g114550	24.672	458	286	15	8	445	8	426	1.39e-24	106
MS.gene47719.t1	MTR_1g040175	25.310	403	234	15	63	434	61	427	4.80e-24	104
MS.gene47719.t1	MTR_1g081660	26.478	389	235	16	47	422	57	407	1.65e-23	103
MS.gene47719.t1	MTR_7g114490	23.454	469	300	14	8	456	8	437	2.15e-23	103
MS.gene47719.t1	MTR_7g114520	25.328	458	283	18	8	444	9	428	1.60e-22	100
MS.gene47719.t1	MTR_3g026950	24.099	444	294	11	31	453	23	444	3.93e-22	99.0
MS.gene47719.t1	MTR_1g053315	24.324	407	252	15	29	413	40	412	2.98e-21	96.7
MS.gene47719.t1	MTR_7g085930	27.342	395	233	19	66	427	59	432	9.60e-21	95.1
MS.gene47719.t1	MTR_7g085990	24.866	374	239	14	66	412	55	413	6.66e-20	92.4
MS.gene47719.t1	MTR_4g055170	24.396	455	294	18	17	449	19	445	4.05e-18	87.0
MS.gene47719.t1	MTR_1g040195	25.068	367	212	14	96	434	71	402	1.06e-17	85.5
MS.gene47719.t1	MTR_3g086020	23.953	430	256	18	7	424	2	372	7.45e-17	82.8
MS.gene47719.t1	MTR_7g084940	25.236	424	257	16	7	412	13	394	7.52e-17	83.2
MS.gene47719.t1	MTR_2g015430	23.940	401	258	17	24	411	18	384	1.17e-16	82.4
MS.gene47719.t1	MTR_3g101900	22.518	413	258	11	27	411	19	397	1.45e-16	82.0
MS.gene47719.t1	MTR_8g079980	23.318	446	279	16	10	437	3	403	2.37e-15	78.2
MS.gene47719.t1	MTR_6g053810	27.476	313	191	13	66	362	71	363	5.83e-15	77.4
MS.gene47719.t1	MTR_3g465990	24.189	339	214	11	49	368	50	364	9.84e-15	76.6
MS.gene47719.t1	MTR_7g114510	24.549	277	177	9	138	413	7	252	2.93e-14	73.9
MS.gene47719.t1	MTR_3g101960	22.812	320	200	12	49	346	48	342	4.62e-14	74.3
MS.gene47719.t1	MTR_4g094842	24.084	382	219	14	61	411	57	398	2.23e-13	72.4
MS.gene47719.t1	MTR_7g110610	31.034	145	87	3	66	201	55	195	3.42e-13	71.2
MS.gene47719.t1	MTR_3g088420	24.372	398	235	16	36	411	32	385	4.81e-13	71.2
MS.gene47719.t1	MTR_3g465980	24.189	339	214	11	49	368	50	364	1.48e-12	69.7
MS.gene47719.t1	MTR_3g101930	22.423	388	241	14	49	409	49	403	2.24e-12	69.3
MS.gene47719.t1	MTR_6g045553	23.385	449	261	17	8	410	12	423	3.35e-12	68.9
MS.gene47719.t1	MTR_3g100150	24.809	262	164	10	31	279	34	275	2.03e-11	66.2
MS.gene47719.t1	MTR_8g041770	23.487	413	249	15	23	411	22	391	3.17e-11	65.5
MS.gene47719.t1	MTR_4g094828	21.891	402	249	15	36	411	37	399	3.88e-11	65.5
MS.gene47719.t1	MTR_4g022825	24.651	430	261	19	6	411	2	392	4.46e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47719.t1	AT1G78990	58.405	464	169	9	8	468	11	453	0.0	514
MS.gene47719.t1	AT1G32910	59.746	472	155	13	8	468	11	458	2.35e-180	513
MS.gene47719.t1	AT5G16410	52.000	475	185	14	8	468	34	479	4.24e-160	462
MS.gene47719.t1	AT1G31490	37.246	443	232	14	8	438	4	412	2.32e-75	243
MS.gene47719.t1	AT5G48930	25.983	458	292	17	10	453	3	427	2.04e-35	137
MS.gene47719.t1	AT5G41040	26.238	404	231	16	31	412	38	396	1.01e-27	115
MS.gene47719.t1	AT5G41040	26.238	404	231	16	31	412	54	412	1.38e-27	115
MS.gene47719.t1	AT2G19070	24.678	466	305	16	18	463	12	451	2.17e-27	114
MS.gene47719.t1	AT5G17540	25.751	466	261	18	4	437	3	415	2.55e-26	111
MS.gene47719.t1	AT5G57840	26.338	467	275	19	10	449	3	427	2.90e-26	111
MS.gene47719.t1	AT3G03480	25.758	462	266	19	8	434	17	436	3.02e-25	108
MS.gene47719.t1	AT3G48720	26.005	423	262	13	3	412	2	386	3.55e-25	107
MS.gene47719.t1	AT3G50270	26.649	379	215	15	60	411	56	398	1.81e-19	91.3
MS.gene47719.t1	AT1G27620	22.195	401	263	13	66	453	58	422	2.07e-19	90.9
MS.gene47719.t1	AT2G40230	23.601	411	261	14	59	453	52	425	8.80e-19	89.0
MS.gene47719.t1	AT1G03390	24.146	439	273	18	2	413	4	409	1.05e-18	89.0
MS.gene47719.t1	AT1G24430	23.765	425	248	17	10	411	5	376	4.78e-18	86.7
MS.gene47719.t1	AT1G28680	26.554	354	218	16	69	411	65	387	1.58e-17	85.1
MS.gene47719.t1	AT3G30280	25.229	436	264	13	10	429	6	395	1.61e-17	85.1
MS.gene47719.t1	AT2G39980	24.096	415	235	12	45	411	47	429	2.90e-17	84.7
MS.gene47719.t1	AT1G28680	26.367	311	197	13	69	368	65	354	1.82e-15	78.6
MS.gene47719.t1	AT5G47950	24.344	419	247	15	10	412	6	370	4.07e-15	77.8
MS.gene47719.t1	AT5G01210	24.479	384	203	14	77	411	73	418	1.28e-14	76.3
MS.gene47719.t1	AT4G15390	24.882	422	259	17	10	412	8	390	4.72e-14	74.3
MS.gene47719.t1	AT4G15390	24.882	422	259	17	10	412	8	390	4.72e-14	74.3
MS.gene47719.t1	AT2G25150	23.706	367	232	12	63	411	64	400	2.60e-13	72.4
MS.gene47719.t1	AT5G67160	24.018	433	255	19	59	470	49	428	5.27e-13	71.2
MS.gene47719.t1	AT1G24420	23.864	440	254	17	7	417	2	389	1.88e-12	69.3
MS.gene47719.t1	AT1G24420	23.864	440	254	17	7	417	2	389	1.88e-12	69.3
MS.gene47719.t1	AT5G47980	21.934	424	269	10	7	410	2	383	6.92e-12	67.8
MS.gene47719.t1	AT3G50300	24.390	369	228	15	63	411	56	393	7.81e-12	67.4
MS.gene47719.t1	AT4G15400	24.208	442	265	16	7	427	2	394	2.03e-11	66.2

Find 127 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGATTATGAATTCATATTT+TGG	0.139298	5.3:-73064923	MS.gene47719:CDS
TTCTGCTTTCTTGTGGAAAA+TGG	0.174817	5.3:-73065047	MS.gene47719:CDS
CCAGAGTCGAAGGTTGATTT+TGG	0.189640	5.3:-73064659	MS.gene47719:CDS
TAGTATATGCGACTTATTAT+TGG	0.243557	5.3:+73065157	None:intergenic
AGCACACCATGTTTCTTCTT+TGG	0.268793	5.3:+73065751	None:intergenic
TTAATATCAGAGTCAGATTC+AGG	0.280399	5.3:+73065187	None:intergenic
GTTTCTTATTATGCCTTTGC+TGG	0.282844	5.3:-73065916	MS.gene47719:CDS
AAGAAACATGTTTGCAGAAT+AGG	0.336073	5.3:+73065375	None:intergenic
GGATGGAAGCTGGGCGTCGT+GGG	0.339618	5.3:+73065266	None:intergenic
TAAGGGACACTTGCACTTCA+TGG	0.343370	5.3:-73064519	MS.gene47719:CDS
CGAAAACAAGGTTGCGTTGT+TGG	0.344546	5.3:+73065304	None:intergenic
CAAACATGTTTCTTGTATCA+TGG	0.346322	5.3:-73065366	MS.gene47719:CDS
GGTGACAGAGGAGCATTTCT+TGG	0.346724	5.3:-73064798	MS.gene47719:CDS
ATTTCTTGGGTTTAATAGAT+TGG	0.349608	5.3:-73064784	MS.gene47719:CDS
GAAGAGCATCGTCCAGTTCC+TGG	0.354253	5.3:-73064758	MS.gene47719:CDS
AGCAGCCACTATTTCTTTGT+TGG	0.356679	5.3:+73066101	None:intergenic
TGCCAATGCCAAGCCCTAAA+AGG	0.358606	5.3:-73064574	MS.gene47719:CDS
AAGAACACACCAACATCAAC+TGG	0.358958	5.3:+73066021	None:intergenic
TTTCCTTGGGGAGGTAGTGC+TGG	0.358995	5.3:-73064605	MS.gene47719:CDS
AGTATATGCGACTTATTATT+GGG	0.359817	5.3:+73065158	None:intergenic
AAGAGATTGGTCTAAAGAAA+TGG	0.367262	5.3:+73064483	None:intergenic
TCAAACACCCCACAATGTTA+TGG	0.372360	5.3:+73065962	None:intergenic
TCAGAGTCAGATTCAGGTTC+AGG	0.387820	5.3:+73065193	None:intergenic
TTTCTTGGGTTTAATAGATT+GGG	0.393187	5.3:-73064783	MS.gene47719:CDS
GAAAACAAGGTTGCGTTGTT+GGG	0.393188	5.3:+73065305	None:intergenic
GATCAAAGACTTGTTATCAT+TGG	0.395387	5.3:+73065327	None:intergenic
ATTTCTTTAGACCAATCTCT+TGG	0.398784	5.3:-73064481	MS.gene47719:CDS
CCAAAATCAACCTTCGACTC+TGG	0.403209	5.3:+73064659	None:intergenic
ATCAAAGACTTGTTATCATT+GGG	0.403427	5.3:+73065328	None:intergenic
TTCCCTTCAACAGATTCATC+TGG	0.408828	5.3:+73065781	None:intergenic
AGAGATTGGTCTAAAGAAAT+GGG	0.420021	5.3:+73064484	None:intergenic
ATTAGGTCAAGGTTTGATAG+TGG	0.421854	5.3:+73066051	None:intergenic
CTAGTCTCCAAGATGTGTAT+TGG	0.425893	5.3:-73066150	None:intergenic
ATCCTTGCCAATCCAGGAAC+TGG	0.426912	5.3:+73064746	None:intergenic
CCAACATTTGTGGTATCTTC+AGG	0.429306	5.3:-73064692	MS.gene47719:CDS
ATCATTGGGCCGAGCTATCT+CGG	0.444473	5.3:+73065342	None:intergenic
GCTTTACATGCACCTTCCTA+AGG	0.445432	5.3:-73064537	MS.gene47719:CDS
GTGACAGAGGAGCATTTCTT+GGG	0.453426	5.3:-73064797	MS.gene47719:CDS
ATGAAGTGCAAGTGTCCCTT+AGG	0.455932	5.3:+73064521	None:intergenic
CATCATTGTTTGAGTTGGCT+AGG	0.456830	5.3:+73065101	None:intergenic
TTTCCACAAGAAAGCAGAAA+AGG	0.464789	5.3:+73065051	None:intergenic
GGTTGCTGAAGCCGCTTCAA+TGG	0.466106	5.3:-73065026	MS.gene47719:CDS
CTCTGCCAATGCAAGAGCAC+TGG	0.470684	5.3:-73066077	MS.gene47719:CDS
ATCATTGTTTGAGTTGGCTA+GGG	0.473433	5.3:+73065102	None:intergenic
TCCAAGATGTGTATTGGAAA+AGG	0.474951	5.3:-73066144	None:intergenic
TGGATGGAAGCTGGGCGTCG+TGG	0.477575	5.3:+73065265	None:intergenic
TTTCTTTAGACCAATCTCTT+GGG	0.478661	5.3:-73064480	MS.gene47719:CDS
ACTACCATCATTGTTTGAGT+TGG	0.491361	5.3:+73065096	None:intergenic
ATTGAAGGATGGATGGAAGC+TGG	0.491396	5.3:+73065256	None:intergenic
ATGAGGTAATCCCAAGAGAT+TGG	0.494084	5.3:+73064470	None:intergenic
CAATAAATCCTTGCCAATCC+AGG	0.495236	5.3:+73064740	None:intergenic
TATTACCAACAAAGAAATAG+TGG	0.495558	5.3:-73066106	MS.gene47719:CDS
TCAGATTCAGGTTCAGGTGG+TGG	0.495798	5.3:+73065199	None:intergenic
GGCACCCACCATAACATTGT+GGG	0.501925	5.3:-73065970	MS.gene47719:CDS
AAACATGTTTCTTGTATCAT+GGG	0.504377	5.3:-73065365	MS.gene47719:CDS
AGGATTTATTGTGGTAGCAC+TGG	0.506494	5.3:-73064728	MS.gene47719:CDS
GTCATAAATTGAAGGATGGA+TGG	0.511206	5.3:+73065249	None:intergenic
GGTTGATAATCCGCTATGTT+GGG	0.513429	5.3:-73064855	MS.gene47719:CDS
AATAAGCCTGTGATTACTAC+TGG	0.513795	5.3:-73065991	MS.gene47719:CDS
TTCTGCTAATCTACCACCAT+AGG	0.514784	5.3:+73064883	None:intergenic
AATCCAGATGAATCTGTTGA+AGG	0.515928	5.3:-73065784	MS.gene47719:CDS
GCATCGTCCAGTTCCTGGAT+TGG	0.519656	5.3:-73064753	MS.gene47719:CDS
ATCCAGATGAATCTGTTGAA+GGG	0.525156	5.3:-73065783	MS.gene47719:CDS
GGTAGCACTGGTGGAGAAGA+AGG	0.530503	5.3:-73064716	MS.gene47719:CDS
TATCATGGGCCGAGATAGCT+CGG	0.539461	5.3:-73065351	MS.gene47719:CDS
ATTTATTGTGGTAGCACTGG+TGG	0.539802	5.3:-73064725	MS.gene47719:CDS
TGGTTGATAATCCGCTATGT+TGG	0.541576	5.3:-73064856	MS.gene47719:CDS
AGAAGAAGGACCAACATTTG+TGG	0.543509	5.3:-73064702	MS.gene47719:CDS
TTGTTATTGTGCAGGCAACT+TGG	0.544767	5.3:-73065453	MS.gene47719:intron
GATTCAGGTTCAGGTGGTGG+AGG	0.546175	5.3:+73065202	None:intergenic
TATGCGACTTATTATTGGGT+CGG	0.548443	5.3:+73065162	None:intergenic
GATGGAAGCTGGGCGTCGTG+GGG	0.548673	5.3:+73065267	None:intergenic
AACACAAACTTTAATTGATG+AGG	0.548741	5.3:+73064453	None:intergenic
TTGAAGGATGGATGGAAGCT+GGG	0.551538	5.3:+73065257	None:intergenic
TTTGGAGGCGGCGGTGACAG+AGG	0.555868	5.3:-73064810	MS.gene47719:CDS
AGTCATCACTTCACCAGCAA+AGG	0.556336	5.3:+73065903	None:intergenic
AGAAGTGACCGTCGAAAACA+AGG	0.557353	5.3:+73065292	None:intergenic
GCCCTAAAAGGAATGGTGAC+TGG	0.557544	5.3:-73064562	MS.gene47719:CDS
GGAAGCCAGTGCTCTTGCAT+TGG	0.564426	5.3:+73066072	None:intergenic
CTTGGGTTTAATAGATTGGG+TGG	0.568431	5.3:-73064780	MS.gene47719:CDS
TGGTAGATTAGCAGAAGAGC+TGG	0.570353	5.3:-73064876	MS.gene47719:CDS
CGATTGTTGCAAAGTAACTC+AGG	0.570367	5.3:+73065865	None:intergenic
CTAGCCAACTCAAACAATGA+TGG	0.574494	5.3:-73065100	MS.gene47719:CDS
AGAACACACCAACATCAACT+GGG	0.575989	5.3:+73066022	None:intergenic
CCTGAAGATACCACAAATGT+TGG	0.579526	5.3:+73064692	None:intergenic
CTTTACATGCACCTTCCTAA+GGG	0.579880	5.3:-73064536	MS.gene47719:CDS
AGAAACAAGAGCCTCACCTA+AGG	0.581520	5.3:+73065933	None:intergenic
ACAAAGGTTTCCAGAGTCGA+AGG	0.585762	5.3:-73064669	MS.gene47719:CDS
ACATACATGTCATAAATTGA+AGG	0.587593	5.3:+73065241	None:intergenic
GGTGGAGGTAGATCAGAGAC+GGG	0.587879	5.3:+73065217	None:intergenic
AACACCCCACAATGTTATGG+TGG	0.588442	5.3:+73065965	None:intergenic
TGGCACCCACCATAACATTG+TGG	0.591108	5.3:-73065971	MS.gene47719:CDS
TTGTGGTATCTTCAGGACAA+AGG	0.593564	5.3:-73064685	MS.gene47719:CDS
ACATGTCATAAATTGAAGGA+TGG	0.593580	5.3:+73065245	None:intergenic
ATGCCAAGCCCTAAAAGGAA+TGG	0.595010	5.3:-73064569	MS.gene47719:CDS
TCTGCTAATCTACCACCATA+GGG	0.595034	5.3:+73064884	None:intergenic
TGGTGGAGGTAGATCAGAGA+CGG	0.600538	5.3:+73065216	None:intergenic
AGTGCAAGTGTCCCTTAGGA+AGG	0.602305	5.3:+73064525	None:intergenic
TTGTGGTTGACGGAAGAAAG+AGG	0.605169	5.3:-73064973	MS.gene47719:CDS
GAGTTACTTTGCAACAATCG+TGG	0.605999	5.3:-73065862	MS.gene47719:CDS
GTCCAGTTCCTGGATTGGCA+AGG	0.607030	5.3:-73064748	MS.gene47719:CDS
AATGTGCTTTCGATTCCCTA+TGG	0.610089	5.3:-73064899	MS.gene47719:CDS
AATAGGCATCCGCTATGCGA+TGG	0.614428	5.3:+73065392	None:intergenic
AAGTAACTCAGGTTCACCCA+TGG	0.618644	5.3:+73065876	None:intergenic
GTGCTTTCGATTCCCTATGG+TGG	0.619185	5.3:-73064896	MS.gene47719:CDS
TGAAGTGATGACTAACTCCA+TGG	0.619717	5.3:-73065893	MS.gene47719:CDS
AAAATGGGGCTTGTGGTTGA+CGG	0.619932	5.3:-73064983	MS.gene47719:CDS
CTTGTTCCAAAGAAGAAACA+TGG	0.620389	5.3:-73065757	MS.gene47719:CDS
TGGGTGCCAGTAGTAATCAC+AGG	0.626482	5.3:+73065985	None:intergenic
ACACCCCACAATGTTATGGT+GGG	0.636435	5.3:+73065966	None:intergenic
GAAATGTGGTGGCTTAGTAG+TGG	0.648560	5.3:-73065428	MS.gene47719:CDS
GGATTGGCAAGGATTTATTG+TGG	0.648587	5.3:-73064737	MS.gene47719:CDS
GAGTCAGATTCAGGTTCAGG+TGG	0.651262	5.3:+73065196	None:intergenic
GCACCCACCATAACATTGTG+GGG	0.654145	5.3:-73065969	MS.gene47719:CDS
AAATGATAAGATCCAAACAC+AGG	0.657847	5.3:+73064626	None:intergenic
TGATAATCCGCTATGTTGGG+TGG	0.663374	5.3:-73064852	MS.gene47719:CDS
GAACACACCAACATCAACTG+GGG	0.669825	5.3:+73066023	None:intergenic
GAGATTGGTCTAAAGAAATG+GGG	0.678557	5.3:+73064485	None:intergenic
AACACACCAACATCAACTGG+GGG	0.688247	5.3:+73066024	None:intergenic
CAGGCAACTTGGATGAAATG+TGG	0.695087	5.3:-73065442	MS.gene47719:CDS
TAGCCAGCACTACCTCCCCA+AGG	0.698597	5.3:+73064602	None:intergenic
GAAGTGATGACTAACTCCAT+GGG	0.699827	5.3:-73065892	MS.gene47719:CDS
GCAACTTGGATGAAATGTGG+TGG	0.707819	5.3:-73065439	MS.gene47719:CDS
GGGACACTTGCACTTCATGG+AGG	0.714604	5.3:-73064516	MS.gene47719:CDS
CGATCTGCCACCCAACATAG+CGG	0.715687	5.3:+73064845	None:intergenic
GGAAGAAAGAGGCTAAGCAA+CGG	0.723389	5.3:-73064962	MS.gene47719:CDS
GTGGAGGTAGATCAGAGACG+GGG	0.788848	5.3:+73065218	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTAAAATAGTATTAATTTCA+GGG	-	chr5.3:73064883-73064902	MS.gene47719:CDS	15.0%
!!!	AAACATAGAAACTTTTTTTT+GGG	-	chr5.3:73064946-73064965	MS.gene47719:CDS	15.0%
!!!	AGATTATGAATTCATATTTT+GGG	-	chr5.3:73065679-73065698	MS.gene47719:intron	15.0%
!!!	ATAATACTAATTCTTTTGTA+AGG	+	chr5.3:73065110-73065129	None:intergenic	15.0%
!!!	TAAACATAGAAACTTTTTTT+TGG	-	chr5.3:73064945-73064964	MS.gene47719:CDS	15.0%
!!!	TAATACTAATTCTTTTGTAA+GGG	+	chr5.3:73065109-73065128	None:intergenic	15.0%
!!	GGTAAAATAGTATTAATTTC+AGG	-	chr5.3:73064882-73064901	MS.gene47719:CDS	20.0%
!!!	GAGATTATGAATTCATATTT+TGG	-	chr5.3:73065678-73065697	MS.gene47719:intron	20.0%
!	AAAACATTGTTGCAAAAATG+GGG	-	chr5.3:73065604-73065623	MS.gene47719:intron	25.0%
!	AAACATGTTTCTTGTATCAT+GGG	-	chr5.3:73065236-73065255	MS.gene47719:CDS	25.0%
!	ACATACATGTCATAAATTGA+AGG	+	chr5.3:73065363-73065382	None:intergenic	25.0%
!	AGTATATGCGACTTATTATT+GGG	+	chr5.3:73065446-73065465	None:intergenic	25.0%
!	ATTTCTTGGGTTTAATAGAT+TGG	-	chr5.3:73065817-73065836	MS.gene47719:CDS	25.0%
!	GAAAACATTGTTGCAAAAAT+GGG	-	chr5.3:73065603-73065622	MS.gene47719:intron	25.0%
!	TAGTATATGCGACTTATTAT+TGG	+	chr5.3:73065447-73065466	None:intergenic	25.0%
!	TATTACCAACAAAGAAATAG+TGG	-	chr5.3:73064495-73064514	MS.gene47719:CDS	25.0%
!	TGAAAACATTGTTGCAAAAA+TGG	-	chr5.3:73065602-73065621	MS.gene47719:intron	25.0%
!	TTTCTTGGGTTTAATAGATT+GGG	-	chr5.3:73065818-73065837	MS.gene47719:CDS	25.0%
!!	AATTATCATTGTTATTGTGC+AGG	-	chr5.3:73065140-73065159	MS.gene47719:CDS	25.0%
!!!	ATCAAAGACTTGTTATCATT+GGG	+	chr5.3:73065276-73065295	None:intergenic	25.0%
	AAATGATAAGATCCAAACAC+AGG	+	chr5.3:73065978-73065997	None:intergenic	30.0%
	AAGAAACATGTTTGCAGAAT+AGG	+	chr5.3:73065229-73065248	None:intergenic	30.0%
	ACATGTCATAAATTGAAGGA+TGG	+	chr5.3:73065359-73065378	None:intergenic	30.0%
	AGGCAAAGAATTATCATCAT+CGG	-	chr5.3:73065051-73065070	MS.gene47719:CDS	30.0%
	ATTTCTTTAGACCAATCTCT+TGG	-	chr5.3:73066120-73066139	MS.gene47719:CDS	30.0%
	CAAACATGTTTCTTGTATCA+TGG	-	chr5.3:73065235-73065254	MS.gene47719:CDS	30.0%
	TTAATATCAGAGTCAGATTC+AGG	+	chr5.3:73065417-73065436	None:intergenic	30.0%
	TTTCTTTAGACCAATCTCTT+GGG	-	chr5.3:73066121-73066140	MS.gene47719:CDS	30.0%
	TTTGAAAAATTCCTTAGGTG+AGG	-	chr5.3:73064657-73064676	MS.gene47719:CDS	30.0%
!	GTTTTCATTATCCATTGAAG+CGG	+	chr5.3:73065589-73065608	None:intergenic	30.0%
!	TAGATATCGACCAAACATTT+TGG	-	chr5.3:73065008-73065027	MS.gene47719:CDS	30.0%
!	TGGATCTTATCATTTTCCTT+GGG	-	chr5.3:73065983-73066002	MS.gene47719:CDS	30.0%
!	TTGGATCTTATCATTTTCCT+TGG	-	chr5.3:73065982-73066001	MS.gene47719:CDS	30.0%
!!	AAGAGATTGGTCTAAAGAAA+TGG	+	chr5.3:73066121-73066140	None:intergenic	30.0%
!!	AGAGATTGGTCTAAAGAAAT+GGG	+	chr5.3:73066120-73066139	None:intergenic	30.0%
!!	GATCAAAGACTTGTTATCAT+TGG	+	chr5.3:73065277-73065296	None:intergenic	30.0%
	AATAAGCCTGTGATTACTAC+TGG	-	chr5.3:73064610-73064629	MS.gene47719:CDS	35.0%
	AATCCAGATGAATCTGTTGA+AGG	-	chr5.3:73064817-73064836	MS.gene47719:CDS	35.0%
	ACTACCATCATTGTTTGAGT+TGG	+	chr5.3:73065508-73065527	None:intergenic	35.0%
	ATCCAGATGAATCTGTTGAA+GGG	-	chr5.3:73064818-73064837	MS.gene47719:CDS	35.0%
	ATTAGGTCAAGGTTTGATAG+TGG	+	chr5.3:73064553-73064572	None:intergenic	35.0%
	CTTGTTCCAAAGAAGAAACA+TGG	-	chr5.3:73064844-73064863	MS.gene47719:CDS	35.0%
	GGGTGTTTGAAAAATTCCTT+AGG	-	chr5.3:73064652-73064671	MS.gene47719:CDS	35.0%
	GTCATAAATTGAAGGATGGA+TGG	+	chr5.3:73065355-73065374	None:intergenic	35.0%
	GTTTCTTATTATGCCTTTGC+TGG	-	chr5.3:73064685-73064704	MS.gene47719:CDS	35.0%
	TATGCGACTTATTATTGGGT+CGG	+	chr5.3:73065442-73065461	None:intergenic	35.0%
	TCTCGAGGGAAAATTAGAAT+AGG	-	chr5.3:73065031-73065050	MS.gene47719:CDS	35.0%
	TTCTGCTTTCTTGTGGAAAA+TGG	-	chr5.3:73065554-73065573	MS.gene47719:intron	35.0%
	TTTCCACAAGAAAGCAGAAA+AGG	+	chr5.3:73065553-73065572	None:intergenic	35.0%
!	AGATCGAGTTCATGAGTTTT+TGG	-	chr5.3:73065773-73065792	MS.gene47719:CDS	35.0%
!	AGTTTTTAAACTTGTGCCCT+TGG	+	chr5.3:73064926-73064945	None:intergenic	35.0%
!	GGATCTTATCATTTTCCTTG+GGG	-	chr5.3:73065984-73066003	MS.gene47719:CDS	35.0%
!	GTTTTTAAACTTGTGCCCTT+GGG	+	chr5.3:73064925-73064944	None:intergenic	35.0%
!	TTTTTAAACTTGTGCCCTTG+GGG	+	chr5.3:73064924-73064943	None:intergenic	35.0%
!!	ATCATTGTTTGAGTTGGCTA+GGG	+	chr5.3:73065502-73065521	None:intergenic	35.0%
!!	GAGATTGGTCTAAAGAAATG+GGG	+	chr5.3:73066119-73066138	None:intergenic	35.0%
	AAGAACACACCAACATCAAC+TGG	+	chr5.3:73064583-73064602	None:intergenic	40.0%
	AATGTGCTTTCGATTCCCTA+TGG	-	chr5.3:73065702-73065721	MS.gene47719:intron	40.0%
	AGAACACACCAACATCAACT+GGG	+	chr5.3:73064582-73064601	None:intergenic	40.0%
	AGAAGAAGGACCAACATTTG+TGG	-	chr5.3:73065899-73065918	MS.gene47719:CDS	40.0%
	AGCACACCATGTTTCTTCTT+TGG	+	chr5.3:73064853-73064872	None:intergenic	40.0%
	AGGATTTATTGTGGTAGCAC+TGG	-	chr5.3:73065873-73065892	MS.gene47719:CDS	40.0%
	ATGAGGTAATCCCAAGAGAT+TGG	+	chr5.3:73066134-73066153	None:intergenic	40.0%
	CAATAAATCCTTGCCAATCC+AGG	+	chr5.3:73065864-73065883	None:intergenic	40.0%
	CCAACATTTGTGGTATCTTC+AGG	-	chr5.3:73065909-73065928	MS.gene47719:CDS	40.0%
	CCTGAAGATACCACAAATGT+TGG	+	chr5.3:73065912-73065931	None:intergenic	40.0%
	CGATTGTTGCAAAGTAACTC+AGG	+	chr5.3:73064739-73064758	None:intergenic	40.0%
	CTAGCCAACTCAAACAATGA+TGG	-	chr5.3:73065501-73065520	MS.gene47719:intron	40.0%
	CTTGGGTTTAATAGATTGGG+TGG	-	chr5.3:73065821-73065840	MS.gene47719:CDS	40.0%
	CTTTACATGCACCTTCCTAA+GGG	-	chr5.3:73066065-73066084	MS.gene47719:CDS	40.0%
	GAAAACAAGGTTGCGTTGTT+GGG	+	chr5.3:73065299-73065318	None:intergenic	40.0%
	GAAGTGATGACTAACTCCAT+GGG	-	chr5.3:73064709-73064728	MS.gene47719:CDS	40.0%
	TCAAACACCCCACAATGTTA+TGG	+	chr5.3:73064642-73064661	None:intergenic	40.0%
	TCTGCTAATCTACCACCATA+GGG	+	chr5.3:73065720-73065739	None:intergenic	40.0%
	TGAAGTGATGACTAACTCCA+TGG	-	chr5.3:73064708-73064727	MS.gene47719:CDS	40.0%
	TTCCCTTCAACAGATTCATC+TGG	+	chr5.3:73064823-73064842	None:intergenic	40.0%
	TTCTGCTAATCTACCACCAT+AGG	+	chr5.3:73065721-73065740	None:intergenic	40.0%
	TTGTGGTATCTTCAGGACAA+AGG	-	chr5.3:73065916-73065935	MS.gene47719:CDS	40.0%
!	AACCAGTCACCATTCCTTTT+AGG	+	chr5.3:73066044-73066063	None:intergenic	40.0%
!	ACCAGTCACCATTCCTTTTA+GGG	+	chr5.3:73066043-73066062	None:intergenic	40.0%
!	AGCAGCCACTATTTCTTTGT+TGG	+	chr5.3:73064503-73064522	None:intergenic	40.0%
!	AGTCCTTTTCTGCTTTCTTG+TGG	-	chr5.3:73065547-73065566	MS.gene47719:intron	40.0%
!	ATTTATTGTGGTAGCACTGG+TGG	-	chr5.3:73065876-73065895	MS.gene47719:CDS	40.0%
!	GAGTTACTTTGCAACAATCG+TGG	-	chr5.3:73064739-73064758	MS.gene47719:CDS	40.0%
!	GGATTGGCAAGGATTTATTG+TGG	-	chr5.3:73065864-73065883	MS.gene47719:CDS	40.0%
!	TCTTATCATTTTCCTTGGGG+AGG	-	chr5.3:73065987-73066006	MS.gene47719:CDS	40.0%
!	TTGTTATTGTGCAGGCAACT+TGG	-	chr5.3:73065148-73065167	MS.gene47719:CDS	40.0%
!!	AAATGTTAACTTGTGCCCCA+AGG	-	chr5.3:73064906-73064925	MS.gene47719:CDS	40.0%
!!	AATGTTAACTTGTGCCCCAA+GGG	-	chr5.3:73064907-73064926	MS.gene47719:CDS	40.0%
!!	ACCAAACATTTTGGTCTCGA+GGG	-	chr5.3:73065017-73065036	MS.gene47719:CDS	40.0%
!!	AGTCGAAGGTTGATTTTGGA+TGG	-	chr5.3:73065946-73065965	MS.gene47719:CDS	40.0%
!!	AGTTCATGAGTTTTTGGAGG+CGG	-	chr5.3:73065779-73065798	MS.gene47719:CDS	40.0%
!!	CATCATTGTTTGAGTTGGCT+AGG	+	chr5.3:73065503-73065522	None:intergenic	40.0%
!!	GGTTGATAATCCGCTATGTT+GGG	-	chr5.3:73065746-73065765	MS.gene47719:intron	40.0%
!!	GTCGAAGGTTGATTTTGGAT+GGG	-	chr5.3:73065947-73065966	MS.gene47719:CDS	40.0%
!!	TCGAAGGTTGATTTTGGATG+GGG	-	chr5.3:73065948-73065967	MS.gene47719:CDS	40.0%
!!	TCGAGTTCATGAGTTTTTGG+AGG	-	chr5.3:73065776-73065795	MS.gene47719:CDS	40.0%
!!	TGGTTGATAATCCGCTATGT+TGG	-	chr5.3:73065745-73065764	MS.gene47719:intron	40.0%
	AAAATGGGGCTTGTGGTTGA+CGG	-	chr5.3:73065618-73065637	MS.gene47719:intron	45.0%
	AACACACCAACATCAACTGG+GGG	+	chr5.3:73064580-73064599	None:intergenic	45.0%
	AACACCCCACAATGTTATGG+TGG	+	chr5.3:73064639-73064658	None:intergenic	45.0%
	AAGTAACTCAGGTTCACCCA+TGG	+	chr5.3:73064728-73064747	None:intergenic	45.0%
	ACAAAGGTTTCCAGAGTCGA+AGG	-	chr5.3:73065932-73065951	MS.gene47719:CDS	45.0%
	ACACCCCACAATGTTATGGT+GGG	+	chr5.3:73064638-73064657	None:intergenic	45.0%
	AGAAACAAGAGCCTCACCTA+AGG	+	chr5.3:73064671-73064690	None:intergenic	45.0%
	AGAAGTGACCGTCGAAAACA+AGG	+	chr5.3:73065312-73065331	None:intergenic	45.0%
	AGTCATCACTTCACCAGCAA+AGG	+	chr5.3:73064701-73064720	None:intergenic	45.0%
	ATGAAGTGCAAGTGTCCCTT+AGG	+	chr5.3:73066083-73066102	None:intergenic	45.0%
	ATGCCAAGCCCTAAAAGGAA+TGG	-	chr5.3:73066032-73066051	MS.gene47719:CDS	45.0%
	ATTGAAGGATGGATGGAAGC+TGG	+	chr5.3:73065348-73065367	None:intergenic	45.0%
	CAGGCAACTTGGATGAAATG+TGG	-	chr5.3:73065159-73065178	MS.gene47719:CDS	45.0%
	CATCAACTGGGGGAAGAATT+AGG	+	chr5.3:73064570-73064589	None:intergenic	45.0%
	CCAAAATCAACCTTCGACTC+TGG	+	chr5.3:73065945-73065964	None:intergenic	45.0%
	CGAAAACAAGGTTGCGTTGT+TGG	+	chr5.3:73065300-73065319	None:intergenic	45.0%
	GAAATGTGGTGGCTTAGTAG+TGG	-	chr5.3:73065173-73065192	MS.gene47719:CDS	45.0%
	GAACACACCAACATCAACTG+GGG	+	chr5.3:73064581-73064600	None:intergenic	45.0%
	GCAACTTGGATGAAATGTGG+TGG	-	chr5.3:73065162-73065181	MS.gene47719:CDS	45.0%
	GCTTTACATGCACCTTCCTA+AGG	-	chr5.3:73066064-73066083	MS.gene47719:CDS	45.0%
	GGAAGAAAGAGGCTAAGCAA+CGG	-	chr5.3:73065639-73065658	MS.gene47719:intron	45.0%
	TAAGGGACACTTGCACTTCA+TGG	-	chr5.3:73066082-73066101	MS.gene47719:CDS	45.0%
	TCCCTCGAGACCAAAATGTT+TGG	+	chr5.3:73065021-73065040	None:intergenic	45.0%
	TGATAATCCGCTATGTTGGG+TGG	-	chr5.3:73065749-73065768	MS.gene47719:CDS	45.0%
	TGGTAGATTAGCAGAAGAGC+TGG	-	chr5.3:73065725-73065744	MS.gene47719:intron	45.0%
	TGTTGCAAAAATGGGGCTTG+TGG	-	chr5.3:73065611-73065630	MS.gene47719:intron	45.0%
	TTGAAGGATGGATGGAAGCT+GGG	+	chr5.3:73065347-73065366	None:intergenic	45.0%
	TTGTGGTTGACGGAAGAAAG+AGG	-	chr5.3:73065628-73065647	MS.gene47719:intron	45.0%
!	ATTCTTCCCCCAGTTGATGT+TGG	-	chr5.3:73064571-73064590	MS.gene47719:CDS	45.0%
!	CACTTTTGACCATCGCATAG+CGG	-	chr5.3:73065200-73065219	MS.gene47719:CDS	45.0%
!	TCAGAGTCAGATTCAGGTTC+AGG	+	chr5.3:73065411-73065430	None:intergenic	45.0%
!!	CCAGAGTCGAAGGTTGATTT+TGG	-	chr5.3:73065942-73065961	MS.gene47719:CDS	45.0%
!!	CGAAGGTTGATTTTGGATGG+GGG	-	chr5.3:73065949-73065968	MS.gene47719:CDS	45.0%
!!	GACCAAACATTTTGGTCTCG+AGG	-	chr5.3:73065016-73065035	MS.gene47719:CDS	45.0%
!!	GTGACAGAGGAGCATTTCTT+GGG	-	chr5.3:73065804-73065823	MS.gene47719:CDS	45.0%
!!!	CAACGCAACCTTGTTTTCGA+CGG	-	chr5.3:73065301-73065320	MS.gene47719:CDS	45.0%
!!!	TCACCATTCCTTTTAGGGCT+TGG	+	chr5.3:73066038-73066057	None:intergenic	45.0%
!!!	TTCCTTTTAGGGCTTGGCAT+TGG	+	chr5.3:73066032-73066051	None:intergenic	45.0%
	AATAGGCATCCGCTATGCGA+TGG	+	chr5.3:73065212-73065231	None:intergenic	50.0%
	AGTGCAAGTGTCCCTTAGGA+AGG	+	chr5.3:73066079-73066098	None:intergenic	50.0%
	ATCATTGGGCCGAGCTATCT+CGG	+	chr5.3:73065262-73065281	None:intergenic	50.0%
	ATCCTTGCCAATCCAGGAAC+TGG	+	chr5.3:73065858-73065877	None:intergenic	50.0%
	CTGGGGGAAGAATTAGGTCA+AGG	+	chr5.3:73064564-73064583	None:intergenic	50.0%
	GCACCCACCATAACATTGTG+GGG	-	chr5.3:73064632-73064651	MS.gene47719:CDS	50.0%
	GCCCTAAAAGGAATGGTGAC+TGG	-	chr5.3:73066039-73066058	MS.gene47719:CDS	50.0%
	GGCACCCACCATAACATTGT+GGG	-	chr5.3:73064631-73064650	MS.gene47719:CDS	50.0%
	GTGCTTTCGATTCCCTATGG+TGG	-	chr5.3:73065705-73065724	MS.gene47719:intron	50.0%
	TATCATGGGCCGAGATAGCT+CGG	-	chr5.3:73065250-73065269	MS.gene47719:CDS	50.0%
	TGCCAATGCCAAGCCCTAAA+AGG	-	chr5.3:73066027-73066046	MS.gene47719:CDS	50.0%
	TGGCACCCACCATAACATTG+TGG	-	chr5.3:73064630-73064649	MS.gene47719:CDS	50.0%
	TGGGTGCCAGTAGTAATCAC+AGG	+	chr5.3:73064619-73064638	None:intergenic	50.0%
	TGGTGGAGGTAGATCAGAGA+CGG	+	chr5.3:73065388-73065407	None:intergenic	50.0%
!	GAGTCAGATTCAGGTTCAGG+TGG	+	chr5.3:73065408-73065427	None:intergenic	50.0%
!	TCAGATTCAGGTTCAGGTGG+TGG	+	chr5.3:73065405-73065424	None:intergenic	50.0%
!!	ACATGGTGTGCTTGCTGTTC+AGG	-	chr5.3:73064861-73064880	MS.gene47719:CDS	50.0%
!!	GGTGACAGAGGAGCATTTCT+TGG	-	chr5.3:73065803-73065822	MS.gene47719:CDS	50.0%
!!	TCATGAGTTTTTGGAGGCGG+CGG	-	chr5.3:73065782-73065801	MS.gene47719:CDS	50.0%
	CGATCTGCCACCCAACATAG+CGG	+	chr5.3:73065759-73065778	None:intergenic	55.0%
	GCATCGTCCAGTTCCTGGAT+TGG	-	chr5.3:73065848-73065867	MS.gene47719:CDS	55.0%
	GGAAGCCAGTGCTCTTGCAT+TGG	+	chr5.3:73064532-73064551	None:intergenic	55.0%
	GGGACACTTGCACTTCATGG+AGG	-	chr5.3:73066085-73066104	MS.gene47719:CDS	55.0%
	GGTGGAGGTAGATCAGAGAC+GGG	+	chr5.3:73065387-73065406	None:intergenic	55.0%
	GGTTGCTGAAGCCGCTTCAA+TGG	-	chr5.3:73065575-73065594	MS.gene47719:intron	55.0%
	GTGGAGGTAGATCAGAGACG+GGG	+	chr5.3:73065386-73065405	None:intergenic	55.0%
	TTTCCTTGGGGAGGTAGTGC+TGG	-	chr5.3:73065996-73066015	MS.gene47719:CDS	55.0%
!	CTCTGCCAATGCAAGAGCAC+TGG	-	chr5.3:73064524-73064543	MS.gene47719:CDS	55.0%
!	GATTCAGGTTCAGGTGGTGG+AGG	+	chr5.3:73065402-73065421	None:intergenic	55.0%
!!	GAAGAGCATCGTCCAGTTCC+TGG	-	chr5.3:73065843-73065862	MS.gene47719:CDS	55.0%
!!	GGTAGCACTGGTGGAGAAGA+AGG	-	chr5.3:73065885-73065904	MS.gene47719:CDS	55.0%
!!	GTCCAGTTCCTGGATTGGCA+AGG	-	chr5.3:73065853-73065872	MS.gene47719:CDS	55.0%
!!!	GGACTCGAGTTTTGAGCGCT+TGG	+	chr5.3:73065532-73065551	None:intergenic	55.0%
	GGATGGGGGAAGCCTGTGTT+TGG	-	chr5.3:73065963-73065982	MS.gene47719:CDS	60.0%
	TAGCCAGCACTACCTCCCCA+AGG	+	chr5.3:73066002-73066021	None:intergenic	60.0%
	TTTGGAGGCGGCGGTGACAG+AGG	-	chr5.3:73065791-73065810	MS.gene47719:CDS	65.0%
!	GATGGAAGCTGGGCGTCGTG+GGG	+	chr5.3:73065337-73065356	None:intergenic	65.0%
!	GGATGGAAGCTGGGCGTCGT+GGG	+	chr5.3:73065338-73065357	None:intergenic	65.0%
!	TGGATGGAAGCTGGGCGTCG+TGG	+	chr5.3:73065339-73065358	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	73064463	73066160	73064463	ID=MS.gene47719
chr5.3	mRNA	73064463	73066160	73064463	ID=MS.gene47719.t1;Parent=MS.gene47719
chr5.3	exon	73065747	73066160	73065747	ID=MS.gene47719.t1.exon1;Parent=MS.gene47719.t1
chr5.3	CDS	73065747	73066160	73065747	ID=cds.MS.gene47719.t1;Parent=MS.gene47719.t1
chr5.3	exon	73064463	73065461	73064463	ID=MS.gene47719.t1.exon2;Parent=MS.gene47719.t1
chr5.3	CDS	73064463	73065461	73064463	ID=cds.MS.gene47719.t1;Parent=MS.gene47719.t1

Gene Sequence

>MS.gene47719

ATGTGTATTGGAAAAGGAGAATTCACAGTGAATATTACCAACAAAGAAATAGTGGCTGCTGCTCTGCCAATGCAAGAGCACTGGCTTCCACTATCAAACCTTGACCTAATTCTTCCCCCAGTTGATGTTGGTGTGTTCTTTTGCTACAATAAGCCTGTGATTACTACTGGCACCCACCATAACATTGTGGGGTGTTTGAAAAATTCCTTAGGTGAGGCTCTTGTTTCTTATTATGCCTTTGCTGGTGAAGTGATGACTAACTCCATGGGTGAACCTGAGTTACTTTGCAACAATCGTGGAGTTGATTTTGTTGAAGCTTTTGCTGATGTTGAGTTACAATCTCTCAATTTGTATAATCCAGATGAATCTGTTGAAGGGAAGCTTGTTCCAAAGAAGAAACATGGTGTGCTTGCTGTTCAGGTAAAATAGTATTAATTTCAGGGAAATGTTAACTTGTGCCCCAAGGGCACAAGTTTAAAAACTAAACATAGAAACTTTTTTTTGGGAATTGTACATTCATTGTATTAAAAGTTTAAAATTTTTAATAGATATCGACCAAACATTTTGGTCTCGAGGGAAAATTAGAATAGGCAAAGAATTATCATCATCGGCATGTAGTTTTTTTAATAATCATTAGTATTATCCCTTACAAAAGAATTAGTATTATAAAGTGTTTTAATTATCATTGTTATTGTGCAGGCAACTTGGATGAAATGTGGTGGCTTAGTAGTGGCTTGCACTTTTGACCATCGCATAGCGGATGCCTATTCTGCAAACATGTTTCTTGTATCATGGGCCGAGATAGCTCGGCCCAATGATAACAAGTCTTTGATCCCAACAACGCAACCTTGTTTTCGACGGTCACTTCTGACCCCACGACGCCCAGCTTCCATCCATCCTTCAATTTATGACATGTATGTCCCCGTCTCTGATCTACCTCCACCACCTGAACCTGAATCTGACTCTGATATTAAAACCGACCCAATAATAAGTCGCATATACTATGTAACAAGCAAAGAACTCAACAACATGCAGTCCCTAGCCAACTCAAACAATGATGGTAGTTCCAAGCGCTCAAAACTCGAGTCCTTTTCTGCTTTCTTGTGGAAAATGGTTGCTGAAGCCGCTTCAATGGATAATGAAAACATTGTTGCAAAAATGGGGCTTGTGGTTGACGGAAGAAAGAGGCTAAGCAACGGTGACAAAAACAAAGAAGAGATTATGAATTCATATTTTGGGAATGTGCTTTCGATTCCCTATGGTGGTAGATTAGCAGAAGAGCTGGTTGATAATCCGCTATGTTGGGTGGCAGATCGAGTTCATGAGTTTTTGGAGGCGGCGGTGACAGAGGAGCATTTCTTGGGTTTAATAGATTGGGTGGAAGAGCATCGTCCAGTTCCTGGATTGGCAAGGATTTATTGTGGTAGCACTGGTGGAGAAGAAGGACCAACATTTGTGGTATCTTCAGGACAAAGGTTTCCAGAGTCGAAGGTTGATTTTGGATGGGGGAAGCCTGTGTTTGGATCTTATCATTTTCCTTGGGGAGGTAGTGCTGGCTATGTTATGCCAATGCCAAGCCCTAAAAGGAATGGTGACTGGTTGCTTTACATGCACCTTCCTAAGGGACACTTGCACTTCATGGAGGCTCAAGCTCCCCATTTCTTTAGACCAATCTCTTGGGATTACCTCATCAATTAA

Protein sequence

>MS.gene47719.t1

MCIGKGEFTVNITNKEIVAAALPMQEHWLPLSNLDLILPPVDVGVFFCYNKPVITTGTHHNIVGCLKNSLGEALVSYYAFAGEVMTNSMGEPELLCNNRGVDFVEAFADVELQSLNLYNPDESVEGKLVPKKKHGVLAATWMKCGGLVVACTFDHRIADAYSANMFLVSWAEIARPNDNKSLIPTTQPCFRRSLLTPRRPASIHPSIYDMYVPVSDLPPPPEPESDSDIKTDPIISRIYYVTSKELNNMQSLANSNNDGSSKRSKLESFSAFLWKMVAEAASMDNENIVAKMGLVVDGRKRLSNGDKNKEEIMNSYFGNVLSIPYGGRLAEELVDNPLCWVADRVHEFLEAAVTEEHFLGLIDWVEEHRPVPGLARIYCGSTGGEEGPTFVVSSGQRFPESKVDFGWGKPVFGSYHFPWGGSAGYVMPMPSPKRNGDWLLYMHLPKGHLHFMEAQAPHFFRPISWDYLIN