AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47716


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47716.t1 MTR_5g090880 93.658 473 30 0 1 473 1 473 0.0 902
MS.gene47716.t1 MTR_5g090830 80.380 474 89 3 1 473 1 471 0.0 793
MS.gene47716.t1 MTR_5g090810 78.903 474 96 3 1 473 1 471 0.0 780
MS.gene47716.t1 MTR_3g450710 62.839 479 159 7 1 471 1 468 0.0 581
MS.gene47716.t1 MTR_3g450690 61.250 480 169 6 1 471 1 472 0.0 581
MS.gene47716.t1 MTR_5g026320 35.394 469 272 13 7 471 15 456 2.24e-74 241
MS.gene47716.t1 MTR_2g064495 26.190 462 288 16 10 456 3 426 1.89e-36 139
MS.gene47716.t1 MTR_2g105330 26.190 462 288 16 10 456 3 426 3.62e-36 139
MS.gene47716.t1 MTR_8g075610 25.828 453 284 16 17 456 1 414 5.98e-36 138
MS.gene47716.t1 MTR_4g007540 26.144 459 285 16 10 452 3 423 3.95e-35 136
MS.gene47716.t1 MTR_8g096530 26.196 439 274 16 31 459 28 426 1.04e-30 124
MS.gene47716.t1 MTR_3g098980 26.904 394 239 14 31 414 35 389 1.84e-27 114
MS.gene47716.t1 MTR_1g040215 26.368 402 233 13 64 437 61 427 3.86e-27 114
MS.gene47716.t1 MTR_3g049150 24.879 414 273 14 60 456 48 440 4.48e-27 113
MS.gene47716.t1 MTR_7g027175 26.241 423 263 17 4 415 9 393 2.42e-26 111
MS.gene47716.t1 MTR_7g085990 26.823 384 231 15 61 414 49 412 4.68e-26 110
MS.gene47716.t1 MTR_7g114560 24.812 399 256 12 64 450 62 428 3.90e-25 108
MS.gene47716.t1 MTR_1g040175 25.123 406 245 13 64 443 61 433 5.58e-25 107
MS.gene47716.t1 MTR_7g114550 25.564 399 247 14 67 450 65 428 9.02e-25 107
MS.gene47716.t1 MTR_4g127650 23.195 457 305 12 28 466 28 456 1.81e-24 105
MS.gene47716.t1 MTR_7g086000 26.667 375 221 14 67 413 60 408 7.94e-24 104
MS.gene47716.t1 MTR_7g114490 24.634 410 261 14 64 459 62 437 2.36e-23 103
MS.gene47716.t1 MTR_4g097090 25.000 456 299 16 28 466 27 456 3.86e-23 102
MS.gene47716.t1 MTR_1g081660 24.814 403 254 17 64 452 70 437 6.82e-23 101
MS.gene47716.t1 MTR_5g076700 25.926 405 239 17 67 450 65 429 6.91e-23 101
MS.gene47716.t1 MTR_7g114470 25.505 396 242 15 64 435 63 429 9.09e-23 100
MS.gene47716.t1 MTR_7g085930 25.459 381 240 13 61 414 53 416 7.91e-22 98.2
MS.gene47716.t1 MTR_3g026950 24.289 457 304 12 10 447 2 435 1.37e-21 97.4
MS.gene47716.t1 MTR_7g114520 26.923 364 223 13 64 416 63 394 1.45e-21 97.4
MS.gene47716.t1 MTR_7g084940 26.526 426 250 17 6 413 12 392 4.24e-19 89.7
MS.gene47716.t1 MTR_1g040320 23.284 408 214 14 64 443 61 397 2.21e-18 87.4
MS.gene47716.t1 MTR_1g053315 23.284 408 254 16 29 414 40 410 8.15e-18 86.3
MS.gene47716.t1 MTR_1g040195 24.796 367 215 14 97 437 71 402 1.08e-17 85.5
MS.gene47716.t1 MTR_3g465990 25.061 411 255 15 49 440 50 426 2.81e-17 84.3
MS.gene47716.t1 MTR_3g465980 25.519 337 211 10 49 369 50 362 3.56e-16 80.9
MS.gene47716.t1 MTR_3g086020 24.823 423 254 21 6 417 1 370 3.71e-16 80.9
MS.gene47716.t1 MTR_8g079980 23.836 365 219 11 67 414 59 381 5.64e-16 80.5
MS.gene47716.t1 MTR_1g040315 26.457 223 141 6 64 278 61 268 1.08e-15 79.3
MS.gene47716.t1 MTR_4g055170 23.716 409 251 13 67 449 69 442 1.26e-15 79.3
MS.gene47716.t1 MTR_3g101900 23.885 381 232 13 67 425 66 410 8.75e-15 76.6
MS.gene47716.t1 MTR_3g070170 22.871 411 258 15 60 439 59 441 1.36e-14 76.3
MS.gene47716.t1 MTR_7g056087 22.796 465 299 17 10 453 4 429 2.00e-14 75.5
MS.gene47716.t1 MTR_2g015430 23.210 405 267 16 21 415 15 385 2.14e-14 75.5
MS.gene47716.t1 MTR_3g101930 24.868 378 218 15 62 412 65 403 4.98e-14 74.3
MS.gene47716.t1 MTR_8g467580 23.853 436 249 20 10 414 3 386 5.08e-14 74.3
MS.gene47716.t1 MTR_4g022720 26.087 368 217 14 67 414 55 387 7.39e-14 73.9
MS.gene47716.t1 MTR_3g112180 23.797 395 228 14 67 429 61 414 1.97e-13 72.4
MS.gene47716.t1 MTR_3g101960 23.118 372 226 12 63 412 64 397 2.19e-13 72.4
MS.gene47716.t1 MTR_3g088420 23.423 444 263 18 36 455 32 422 9.69e-13 70.5
MS.gene47716.t1 MTR_4g022290 24.538 379 211 16 67 414 60 394 1.23e-12 70.1
MS.gene47716.t1 MTR_7g114510 24.000 275 177 8 143 416 9 252 4.31e-12 67.4
MS.gene47716.t1 MTR_6g053810 25.633 316 195 13 67 365 71 363 4.61e-12 68.2
MS.gene47716.t1 MTR_4g094828 22.395 451 271 17 36 455 37 439 1.23e-11 67.0
MS.gene47716.t1 MTR_3g465930 23.547 327 202 9 67 372 69 368 2.37e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47716.t1 AT1G78990 54.292 466 188 9 8 471 11 453 5.03e-162 466
MS.gene47716.t1 AT1G32910 54.797 469 186 9 8 471 11 458 1.74e-160 462
MS.gene47716.t1 AT5G16410 50.633 474 196 12 8 471 34 479 1.00e-150 438
MS.gene47716.t1 AT1G31490 35.639 477 254 15 8 471 4 440 8.41e-76 244
MS.gene47716.t1 AT5G48930 26.681 461 288 16 10 456 3 427 3.16e-35 136
MS.gene47716.t1 AT5G41040 26.329 395 245 13 31 415 38 396 5.84e-28 116
MS.gene47716.t1 AT5G41040 26.329 395 245 13 31 415 54 412 7.72e-28 115
MS.gene47716.t1 AT5G57840 25.270 463 288 15 10 452 3 427 1.07e-26 112
MS.gene47716.t1 AT3G26040 25.463 432 241 19 10 414 3 380 5.41e-25 107
MS.gene47716.t1 AT5G17540 25.541 462 270 19 4 440 3 415 1.36e-24 106
MS.gene47716.t1 AT5G63560 25.893 448 252 18 8 425 5 402 1.79e-23 102
MS.gene47716.t1 AT3G03480 25.000 464 274 17 6 437 15 436 6.54e-23 101
MS.gene47716.t1 AT3G48720 24.784 464 299 16 3 456 2 425 2.11e-22 99.8
MS.gene47716.t1 AT1G65450 26.111 360 222 13 67 413 72 400 2.75e-21 96.7
MS.gene47716.t1 AT1G28680 27.475 404 242 20 26 415 22 388 4.60e-21 95.9
MS.gene47716.t1 AT1G24430 26.492 419 247 18 10 414 5 376 5.68e-21 95.5
MS.gene47716.t1 AT2G40230 25.432 405 243 14 62 448 54 417 1.95e-20 94.0
MS.gene47716.t1 AT2G19070 22.689 476 321 15 10 466 4 451 3.74e-20 93.2
MS.gene47716.t1 AT5G67160 25.814 430 245 19 64 473 53 428 4.75e-20 92.8
MS.gene47716.t1 AT1G27620 22.738 409 253 13 67 456 58 422 4.05e-19 90.1
MS.gene47716.t1 AT3G30280 24.318 403 249 14 27 414 25 386 1.93e-17 84.7
MS.gene47716.t1 AT5G47950 23.060 451 276 15 19 456 17 409 1.02e-15 79.3
MS.gene47716.t1 AT1G24420 25.882 425 253 14 6 414 1 379 3.30e-15 78.2
MS.gene47716.t1 AT1G24420 25.882 425 253 14 6 414 1 379 3.30e-15 78.2
MS.gene47716.t1 AT4G15390 25.887 282 159 10 27 292 27 274 9.14e-15 76.6
MS.gene47716.t1 AT4G15390 25.887 282 159 10 27 292 27 274 9.14e-15 76.6
MS.gene47716.t1 AT5G38130 22.704 392 243 14 61 430 73 426 2.48e-14 75.5
MS.gene47716.t1 AT5G23970 23.077 442 279 16 10 439 3 395 2.82e-14 75.1
MS.gene47716.t1 AT3G50270 24.064 374 229 16 63 414 58 398 5.21e-14 74.3
MS.gene47716.t1 AT5G67150 25.266 376 221 18 63 414 57 396 6.53e-14 73.9
MS.gene47716.t1 AT2G25150 24.065 428 250 14 16 414 19 400 1.07e-13 73.6
MS.gene47716.t1 AT5G47980 22.246 472 288 20 6 450 1 420 9.00e-13 70.5
MS.gene47716.t1 AT5G42830 22.933 375 236 14 61 415 58 399 2.56e-12 68.9
MS.gene47716.t1 AT3G50300 23.592 373 228 16 64 414 56 393 1.08e-11 67.0
MS.gene47716.t1 AT1G65450 25.191 262 160 10 157 413 6 236 4.33e-11 63.9

Find 127 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAGTCAAAGGTAGATTT+TGG 0.197134 5.3:-73089289 MS.gene47716:CDS
GGATGGAAGGTGGGTGTCTT+GGG 0.229374 5.3:+73089896 None:intergenic
TGCAACAACATTTCCACTAT+TGG 0.271768 5.3:+73089636 None:intergenic
CCGACATTTGTGGTGTCTTC+TGG 0.272565 5.3:-73089322 MS.gene47716:CDS
GAGTTTATGAGTTCGTATTT+TGG 0.286533 5.3:-73089553 MS.gene47716:CDS
ACACCCCACAATAGTGTTAT+TGG 0.294608 5.3:+73090477 None:intergenic
GTTTCTTATTACGTCTTTGC+TGG 0.306691 5.3:-73090427 MS.gene47716:CDS
TCTGATTCGGGCTTGGGTTC+AGG 0.310598 5.3:+73089823 None:intergenic
TTAATATCTGATTCGGGCTT+GGG 0.319670 5.3:+73089817 None:intergenic
TTTAATATCTGATTCGGGCT+TGG 0.321191 5.3:+73089816 None:intergenic
GAAGAGCATCGACCGGTTCC+AGG 0.324397 5.3:-73089388 MS.gene47716:CDS
TGGATGGAAGGTGGGTGTCT+TGG 0.324811 5.3:+73089895 None:intergenic
TAAGGAATACTTGCACTTCA+TGG 0.338830 5.3:-73089149 MS.gene47716:CDS
ATTTCTTGGGACTAATTGAT+TGG 0.344863 5.3:-73089414 MS.gene47716:CDS
AGGATTTATTGCGGAGTTCC+TGG 0.347311 5.3:-73089358 MS.gene47716:CDS
CAAACATGTTTCTTGTATCA+TGG 0.350145 5.3:-73089996 MS.gene47716:CDS
TTCTGCTTTCTTGTGGAAGA+TGG 0.357240 5.3:-73089677 MS.gene47716:CDS
TGCCAATGCCTAGTCCTAAA+AGG 0.369732 5.3:-73089204 MS.gene47716:CDS
ATGAGATTGGTCTAAAGAAA+TGG 0.371643 5.3:+73089113 None:intergenic
AAGAACACACCAATACTAAC+AGG 0.372400 5.3:+73090535 None:intergenic
AAGAAACATGTTTGTAGAAT+AGG 0.374201 5.3:+73090005 None:intergenic
GGACACGAAAGAACATTTCT+TGG 0.384547 5.3:-73089428 MS.gene47716:CDS
TTCCCTTCAATGGTTTCCTC+TGG 0.385000 5.3:+73090292 None:intergenic
TGGAACAAGCTTCCCTTCAA+TGG 0.386738 5.3:+73090282 None:intergenic
AGCTGCAACTACCTCCTCTT+TGG 0.387741 5.3:+73090615 None:intergenic
ACATGGTGTGCTTGCTGTTC+AGG 0.388373 5.3:-73090251 MS.gene47716:intron
ATTCCTTGTGGTGGTGATGC+TGG 0.393172 5.3:-73089235 MS.gene47716:CDS
TTACGTCTTTGCTGGTGAAA+TGG 0.396605 5.3:-73090419 MS.gene47716:CDS
TCGGGCTTGGGTTCAGGTGG+AGG 0.406362 5.3:+73089829 None:intergenic
GTGGGTGTCTTGGGGTCAAA+AGG 0.407163 5.3:+73089905 None:intergenic
CAATAAATCCTTGCCAGCCC+TGG 0.423385 5.3:+73089370 None:intergenic
CGCTCTGTAACCCAACATAA+TGG 0.424141 5.3:+73089475 None:intergenic
TGAGATTGGTCTAAAGAAAT+GGG 0.424402 5.3:+73089114 None:intergenic
ATGAATCCTATGATTACTAT+TGG 0.425230 5.3:-73090505 MS.gene47716:CDS
CTAATCACTAAAATGAGTAT+TGG 0.430164 5.3:-73090664 None:intergenic
TGTTGAAAAGCCATTATGTT+GGG 0.431386 5.3:-73089485 MS.gene47716:CDS
CCAAAATCTACCTTTGACTC+TGG 0.435822 5.3:+73089289 None:intergenic
GCATCGACCGGTTCCAGGGC+TGG 0.439356 5.3:-73089383 MS.gene47716:CDS
ATCCTTGCCAGCCCTGGAAC+CGG 0.444779 5.3:+73089376 None:intergenic
GCTTCCACTATTATTTGTGT+TGG 0.455348 5.3:+73089726 None:intergenic
CCTATGTTCTTGCATTGCCT+TGG 0.455581 5.3:+73090591 None:intergenic
GACACGAAAGAACATTTCTT+GGG 0.462909 5.3:-73089427 MS.gene47716:CDS
ACCACCAATAACACTATTGT+GGG 0.470153 5.3:-73090481 MS.gene47716:CDS
TTTCTTGGGACTAATTGATT+GGG 0.478579 5.3:-73089413 MS.gene47716:CDS
TCTATTAATTTACAACTGTA+GGG 0.479476 5.3:+73089514 None:intergenic
ATGAAGTGCAAGTATTCCTT+AGG 0.482803 5.3:+73089151 None:intergenic
GGCTTGGGTTCAGGTGGAGG+AGG 0.482969 5.3:+73089832 None:intergenic
ATTGCTCGAGCGGGCTATCT+CGG 0.484103 5.3:+73089972 None:intergenic
ATTTCTTTAGACCAATCTCA+TGG 0.487418 5.3:-73089111 MS.gene47716:CDS
GTCCTAAAAGGAATGGTGAT+TGG 0.487820 5.3:-73089192 MS.gene47716:CDS
AGAAGGTAATCCCATGAGAT+TGG 0.494084 5.3:+73089100 None:intergenic
TTCTATTAATTTACAACTGT+AGG 0.494706 5.3:+73089513 None:intergenic
GACCGGTTCCAGGGCTGGCA+AGG 0.497114 5.3:-73089378 MS.gene47716:CDS
CGGTTGTTGCAAAGTAGCTC+AGG 0.499190 5.3:+73090376 None:intergenic
AAACATGTTTCTTGTATCAT+GGG 0.502267 5.3:-73089995 MS.gene47716:CDS
TCAGCCGCTTCAACCAATAG+TGG 0.504854 5.3:-73089649 MS.gene47716:CDS
AGTACATGCGACTTATTAGC+TGG 0.506554 5.3:+73089788 None:intergenic
GGATCTTATCATATTCCTTG+TGG 0.513474 5.3:-73089247 MS.gene47716:CDS
GATGGAAGGTGGGTGTCTTG+GGG 0.526099 5.3:+73089897 None:intergenic
CTAGCCAACACAAATAATAG+TGG 0.526578 5.3:-73089730 MS.gene47716:CDS
CACCACCAATAACACTATTG+TGG 0.528613 5.3:-73090482 MS.gene47716:CDS
GTGAAGGATGGATGGAAGGT+GGG 0.534125 5.3:+73089887 None:intergenic
GTCATAGAGTGAAGGATGGA+TGG 0.535942 5.3:+73089879 None:intergenic
AAGCCAGAGGAAACCATTGA+AGG 0.538348 5.3:-73090295 MS.gene47716:CDS
GATTCGGGCTTGGGTTCAGG+TGG 0.543751 5.3:+73089826 None:intergenic
GATCTACATGAATCTTCCTA+AGG 0.548247 5.3:-73089167 MS.gene47716:CDS
AGAAACAAGAGCCTCAGCTA+AGG 0.548714 5.3:+73090444 None:intergenic
TTGTTGAAAAGCCATTATGT+TGG 0.550086 5.3:-73089486 MS.gene47716:CDS
GTCGGTCCATCTGCTCCACC+AGG 0.550954 5.3:+73089340 None:intergenic
TGGGTGTCTTGGGGTCAAAA+GGG 0.554761 5.3:+73089906 None:intergenic
GTATAATTGTGCAGGCAACT+TGG 0.557460 5.3:-73090083 MS.gene47716:intron
GGAGGTAGTTGCAGCTACCA+AGG 0.558424 5.3:-73090608 MS.gene47716:CDS
AGTGAAGGATGGATGGAAGG+TGG 0.560310 5.3:+73089886 None:intergenic
CCAAGGCAATGCAAGAACAT+AGG 0.562331 5.3:-73090591 MS.gene47716:CDS
GTGGTGCCAATAGTAATCAT+AGG 0.562421 5.3:+73090499 None:intergenic
TGGAGGAGGTAGATCCGAGA+TGG 0.563260 5.3:+73089846 None:intergenic
AATATGATAAGATCCAACTA+CGG 0.564107 5.3:+73089255 None:intergenic
TAGAGTGAAGGATGGATGGA+AGG 0.564472 5.3:+73089883 None:intergenic
CAGGCAACTTGGATGAAATG+TGG 0.566561 5.3:-73090072 MS.gene47716:CDS
ATTTCCACTATTGGTTGAAG+CGG 0.566759 5.3:+73089645 None:intergenic
CTTGGGACTAATTGATTGGG+TGG 0.567048 5.3:-73089410 MS.gene47716:CDS
AGACTTGTTATTGCTCGAGC+GGG 0.569461 5.3:+73089963 None:intergenic
ACAAAGATTTCCAGAGTCAA+AGG 0.570544 5.3:-73089299 MS.gene47716:CDS
GGAGTTCCTGGTGGAGCAGA+TGG 0.571944 5.3:-73089346 MS.gene47716:CDS
TGAAATGGTCACTAACTCCA+TGG 0.576847 5.3:-73090404 MS.gene47716:CDS
AGGCAACTTGGATGAAATGT+GGG 0.579164 5.3:-73090071 MS.gene47716:CDS
AGCCAGAGGAAACCATTGAA+GGG 0.581302 5.3:-73090294 MS.gene47716:CDS
AAAAGGGACCTCTGAAAACA+AGG 0.581419 5.3:+73089922 None:intergenic
CCCCACAATAGTGTTATTGG+TGG 0.583667 5.3:+73090480 None:intergenic
TCTTATCATATTCCTTGTGG+TGG 0.583934 5.3:-73089244 MS.gene47716:CDS
AAGAGCATCGACCGGTTCCA+GGG 0.585377 5.3:-73089387 MS.gene47716:CDS
TTTCTTTAGACCAATCTCAT+GGG 0.587261 5.3:-73089110 MS.gene47716:CDS
ATGCCTAGTCCTAAAAGGAA+TGG 0.590231 5.3:-73089199 MS.gene47716:CDS
ATATGATAAGATCCAACTAC+GGG 0.599022 5.3:+73089256 None:intergenic
TGGCATGCAACTTTGACCAT+CGG 0.600034 5.3:-73090038 MS.gene47716:CDS
AAGACTTGTTATTGCTCGAG+CGG 0.603884 5.3:+73089962 None:intergenic
AAGTAGCTCAGGTTCACCCA+TGG 0.604674 5.3:+73090387 None:intergenic
ACTTTGCAACAACCGTGGTG+TGG 0.609718 5.3:-73090368 MS.gene47716:CDS
TTGGGTGGAAGAGCATCGAC+CGG 0.614426 5.3:-73089395 MS.gene47716:CDS
AGCAGATGGACCGACATTTG+TGG 0.619293 5.3:-73089332 MS.gene47716:CDS
AATAGGCGTCCGCTATCCGA+TGG 0.620621 5.3:+73090022 None:intergenic
TGACATGTACATCTCCATCT+CGG 0.620929 5.3:-73089860 MS.gene47716:CDS
ATGTACATGTCATAGAGTGA+AGG 0.625954 5.3:+73089871 None:intergenic
CAGTGTGAATATTACCAAAG+AGG 0.626621 5.3:-73090629 MS.gene47716:CDS
CAACTTTGACCATCGGATAG+CGG 0.635631 5.3:-73090031 MS.gene47716:CDS
CCAGAAGACACCACAAATGT+CGG 0.636633 5.3:+73089322 None:intergenic
CTTGTTCCAAAGAAAACACA+TGG 0.639769 5.3:-73090268 MS.gene47716:CDS
ATTTATTGCGGAGTTCCTGG+TGG 0.643183 5.3:-73089355 MS.gene47716:CDS
GAACACACCAATACTAACAG+GGG 0.649933 5.3:+73090537 None:intergenic
AAAATGGGGATTGTGGTCGA+CGG 0.658600 5.3:-73089613 MS.gene47716:CDS
GGAAGAATGAGATTAAGCAA+TGG 0.658733 5.3:-73089592 MS.gene47716:CDS
TGTGAATATTACCAAAGAGG+AGG 0.660805 5.3:-73090626 MS.gene47716:CDS
GCAACTTGGATGAAATGTGG+GGG 0.664532 5.3:-73090069 MS.gene47716:CDS
AGAACACACCAATACTAACA+GGG 0.664943 5.3:+73090536 None:intergenic
TCTCAATTTGCATAAGCCAG+AGG 0.668470 5.3:-73090308 MS.gene47716:CDS
GGGCTGGCAAGGATTTATTG+CGG 0.669488 5.3:-73089367 MS.gene47716:CDS
GAAATGGTCACTAACTCCAT+GGG 0.670922 5.3:-73090403 MS.gene47716:CDS
ACATGTCATAGAGTGAAGGA+TGG 0.670976 5.3:+73089875 None:intergenic
TAGCCAGCATCACCACCACA+AGG 0.673907 5.3:+73089232 None:intergenic
GGCAACTTGGATGAAATGTG+GGG 0.674719 5.3:-73090070 MS.gene47716:CDS
GAGATTGGTCTAAAGAAATG+GGG 0.682938 5.3:+73089115 None:intergenic
GGAATACTTGCACTTCATGG+AGG 0.690260 5.3:-73089146 MS.gene47716:CDS
CCACCAATAACACTATTGTG+GGG 0.693360 5.3:-73090480 MS.gene47716:CDS
AACACACCAATACTAACAGG+GGG 0.696942 5.3:+73090538 None:intergenic
GAGCTACTTTGCAACAACCG+TGG 0.705966 5.3:-73090373 MS.gene47716:CDS
CAACTTGGATGAAATGTGGG+GGG 0.712514 5.3:-73090068 MS.gene47716:CDS
CTTCAACAAAATCCACACCA+CGG 0.748079 5.3:+73090356 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATTATTATCATTATGC+AGG - chr5.3:73089585-73089604 MS.gene47716:CDS 20.0%
!! TCTATTAATTTACAACTGTA+GGG + chr5.3:73090234-73090253 None:intergenic 20.0%
!! TTCTATTAATTTACAACTGT+AGG + chr5.3:73090235-73090254 None:intergenic 20.0%
!!! GTTTGTTTTAATATCTGATT+CGG + chr5.3:73089938-73089957 None:intergenic 20.0%
!!! TTTGTTTTAATATCTGATTC+GGG + chr5.3:73089937-73089956 None:intergenic 20.0%
! AAACATGTTTCTTGTATCAT+GGG - chr5.3:73089750-73089769 MS.gene47716:CDS 25.0%
! AAGAAACATGTTTGTAGAAT+AGG + chr5.3:73089743-73089762 None:intergenic 25.0%
! AATATGATAAGATCCAACTA+CGG + chr5.3:73090493-73090512 None:intergenic 25.0%
! AATTGTATGTATAATTGTGC+AGG - chr5.3:73089654-73089673 MS.gene47716:CDS 25.0%
! ATGAATCCTATGATTACTAT+TGG - chr5.3:73089240-73089259 MS.gene47716:CDS 25.0%
!! AGTTTATGAGTTCGTATTTT+GGG - chr5.3:73090193-73090212 MS.gene47716:intron 25.0%
!!! ACAAAAACATTTTGATCTCA+AGG - chr5.3:73089545-73089564 MS.gene47716:CDS 25.0%
!!! CAAAAACATTTTGATCTCAA+GGG - chr5.3:73089546-73089565 MS.gene47716:CDS 25.0%
ATATGATAAGATCCAACTAC+GGG + chr5.3:73090492-73090511 None:intergenic 30.0%
ATTTCTTGGGACTAATTGAT+TGG - chr5.3:73090331-73090350 MS.gene47716:CDS 30.0%
ATTTCTTTAGACCAATCTCA+TGG - chr5.3:73090634-73090653 MS.gene47716:CDS 30.0%
CAAACATGTTTCTTGTATCA+TGG - chr5.3:73089749-73089768 MS.gene47716:CDS 30.0%
GAAATGTTGTTGCAAAAATG+GGG - chr5.3:73090118-73090137 MS.gene47716:intron 30.0%
GGAAATGTTGTTGCAAAAAT+GGG - chr5.3:73090117-73090136 MS.gene47716:intron 30.0%
TGGAAATGTTGTTGCAAAAA+TGG - chr5.3:73090116-73090135 MS.gene47716:intron 30.0%
TGTTGAAAAGCCATTATGTT+GGG - chr5.3:73090260-73090279 MS.gene47716:CDS 30.0%
TTGTTGAAAAGCCATTATGT+TGG - chr5.3:73090259-73090278 MS.gene47716:CDS 30.0%
TTTCTTGGGACTAATTGATT+GGG - chr5.3:73090332-73090351 MS.gene47716:CDS 30.0%
TTTCTTTAGACCAATCTCAT+GGG - chr5.3:73090635-73090654 MS.gene47716:CDS 30.0%
! AATCATTTTCTGCTTTCTTG+TGG - chr5.3:73090061-73090080 MS.gene47716:CDS 30.0%
! GAGTTTATGAGTTCGTATTT+TGG - chr5.3:73090192-73090211 MS.gene47716:intron 30.0%
!! ATGAGATTGGTCTAAAGAAA+TGG + chr5.3:73090635-73090654 None:intergenic 30.0%
!! TGAGATTGGTCTAAAGAAAT+GGG + chr5.3:73090634-73090653 None:intergenic 30.0%
AAGAACACACCAATACTAAC+AGG + chr5.3:73089213-73089232 None:intergenic 35.0%
ACAAAGATTTCCAGAGTCAA+AGG - chr5.3:73090446-73090465 MS.gene47716:CDS 35.0%
ACCACCAATAACACTATTGT+GGG - chr5.3:73089264-73089283 MS.gene47716:CDS 35.0%
AGAACACACCAATACTAACA+GGG + chr5.3:73089212-73089231 None:intergenic 35.0%
ATGAAGTGCAAGTATTCCTT+AGG + chr5.3:73090597-73090616 None:intergenic 35.0%
ATGTACATGTCATAGAGTGA+AGG + chr5.3:73089877-73089896 None:intergenic 35.0%
CAGTGTGAATATTACCAAAG+AGG - chr5.3:73089116-73089135 MS.gene47716:CDS 35.0%
CTAGCCAACACAAATAATAG+TGG - chr5.3:73090015-73090034 MS.gene47716:CDS 35.0%
CTTGTTCCAAAGAAAACACA+TGG - chr5.3:73089477-73089496 MS.gene47716:CDS 35.0%
GACACGAAAGAACATTTCTT+GGG - chr5.3:73090318-73090337 MS.gene47716:CDS 35.0%
GATCTACATGAATCTTCCTA+AGG - chr5.3:73090578-73090597 MS.gene47716:CDS 35.0%
GCTTCCACTATTATTTGTGT+TGG + chr5.3:73090022-73090041 None:intergenic 35.0%
GGAAGAATGAGATTAAGCAA+TGG - chr5.3:73090153-73090172 MS.gene47716:intron 35.0%
GGATCTTATCATATTCCTTG+TGG - chr5.3:73090498-73090517 MS.gene47716:CDS 35.0%
GTTTCTTATTACGTCTTTGC+TGG - chr5.3:73089318-73089337 MS.gene47716:CDS 35.0%
TAAGGAATACTTGCACTTCA+TGG - chr5.3:73090596-73090615 MS.gene47716:CDS 35.0%
TCTTATCATATTCCTTGTGG+TGG - chr5.3:73090501-73090520 MS.gene47716:CDS 35.0%
TGCAACAACATTTCCACTAT+TGG + chr5.3:73090112-73090131 None:intergenic 35.0%
TGTGAATATTACCAAAGAGG+AGG - chr5.3:73089119-73089138 MS.gene47716:CDS 35.0%
TTTGAAAAACTCCTTAGCTG+AGG - chr5.3:73089290-73089309 MS.gene47716:CDS 35.0%
! AACCAATCACCATTCCTTTT+AGG + chr5.3:73090558-73090577 None:intergenic 35.0%
! AGTCAAAGGTAGATTTTGGA+TGG - chr5.3:73090460-73090479 MS.gene47716:CDS 35.0%
! ATTTCCACTATTGGTTGAAG+CGG + chr5.3:73090103-73090122 None:intergenic 35.0%
! GTCAAAGGTAGATTTTGGAT+GGG - chr5.3:73090461-73090480 MS.gene47716:CDS 35.0%
! TCAAAGGTAGATTTTGGATG+GGG - chr5.3:73090462-73090481 MS.gene47716:CDS 35.0%
!! GAGATTGGTCTAAAGAAATG+GGG + chr5.3:73090633-73090652 None:intergenic 35.0%
!! TTAATATCTGATTCGGGCTT+GGG + chr5.3:73089931-73089950 None:intergenic 35.0%
!! TTTAATATCTGATTCGGGCT+TGG + chr5.3:73089932-73089951 None:intergenic 35.0%
!!! AGAGCGAGTTCATGATTTTT+TGG - chr5.3:73090287-73090306 MS.gene47716:CDS 35.0%
AACACACCAATACTAACAGG+GGG + chr5.3:73089210-73089229 None:intergenic 40.0%
ACACCCCACAATAGTGTTAT+TGG + chr5.3:73089271-73089290 None:intergenic 40.0%
ACATGTCATAGAGTGAAGGA+TGG + chr5.3:73089873-73089892 None:intergenic 40.0%
AGAAGGTAATCCCATGAGAT+TGG + chr5.3:73090648-73090667 None:intergenic 40.0%
AGGCAACTTGGATGAAATGT+GGG - chr5.3:73089674-73089693 MS.gene47716:CDS 40.0%
AGTACATGCGACTTATTAGC+TGG + chr5.3:73089960-73089979 None:intergenic 40.0%
ATGCCTAGTCCTAAAAGGAA+TGG - chr5.3:73090546-73090565 MS.gene47716:CDS 40.0%
CACCACCAATAACACTATTG+TGG - chr5.3:73089263-73089282 MS.gene47716:CDS 40.0%
CCAAAATCTACCTTTGACTC+TGG + chr5.3:73090459-73090478 None:intergenic 40.0%
CCACCAATAACACTATTGTG+GGG - chr5.3:73089265-73089284 MS.gene47716:CDS 40.0%
CTTCAACAAAATCCACACCA+CGG + chr5.3:73089392-73089411 None:intergenic 40.0%
GAAATGGTCACTAACTCCAT+GGG - chr5.3:73089342-73089361 MS.gene47716:CDS 40.0%
GAACACACCAATACTAACAG+GGG + chr5.3:73089211-73089230 None:intergenic 40.0%
GGACACGAAAGAACATTTCT+TGG - chr5.3:73090317-73090336 MS.gene47716:CDS 40.0%
GTATAATTGTGCAGGCAACT+TGG - chr5.3:73089662-73089681 MS.gene47716:CDS 40.0%
GTCCTAAAAGGAATGGTGAT+TGG - chr5.3:73090553-73090572 MS.gene47716:CDS 40.0%
TCTCAATTTGCATAAGCCAG+AGG - chr5.3:73089437-73089456 MS.gene47716:CDS 40.0%
TGAAATGGTCACTAACTCCA+TGG - chr5.3:73089341-73089360 MS.gene47716:CDS 40.0%
TGACATGTACATCTCCATCT+CGG - chr5.3:73089885-73089904 MS.gene47716:CDS 40.0%
TGTTGCAAAAATGGGGATTG+TGG - chr5.3:73090125-73090144 MS.gene47716:intron 40.0%
TTACGTCTTTGCTGGTGAAA+TGG - chr5.3:73089326-73089345 MS.gene47716:CDS 40.0%
TTCTGCTTTCTTGTGGAAGA+TGG - chr5.3:73090068-73090087 MS.gene47716:CDS 40.0%
! AAAAGGGACCTCTGAAAACA+AGG + chr5.3:73089826-73089845 None:intergenic 40.0%
! AGCACACCATGTGTTTTCTT+TGG + chr5.3:73089486-73089505 None:intergenic 40.0%
! CAAAGGTAGATTTTGGATGG+GGG - chr5.3:73090463-73090482 MS.gene47716:CDS 40.0%
! CCAGAGTCAAAGGTAGATTT+TGG - chr5.3:73090456-73090475 MS.gene47716:CDS 40.0%
! GTGGTGCCAATAGTAATCAT+AGG + chr5.3:73089249-73089268 None:intergenic 40.0%
!! AAGACTTGTTATTGCTCGAG+CGG + chr5.3:73089786-73089805 None:intergenic 40.0%
!!! CAACACAACCTTGTTTTCAG+AGG - chr5.3:73089815-73089834 MS.gene47716:CDS 40.0%
!!! GCGAGTTCATGATTTTTTGG+AGG - chr5.3:73090290-73090309 MS.gene47716:CDS 40.0%
!!! TCACCATTCCTTTTAGGACT+AGG + chr5.3:73090552-73090571 None:intergenic 40.0%
!!! TCATGATTTTTTGGAGGCTG+CGG - chr5.3:73090296-73090315 MS.gene47716:CDS 40.0%
!!! TTCCTTTTAGGACTAGGCAT+TGG + chr5.3:73090546-73090565 None:intergenic 40.0%
AAAATGGGGATTGTGGTCGA+CGG - chr5.3:73090132-73090151 MS.gene47716:intron 45.0%
AAGCCAGAGGAAACCATTGA+AGG - chr5.3:73089450-73089469 MS.gene47716:CDS 45.0%
AGAAACAAGAGCCTCAGCTA+AGG + chr5.3:73089304-73089323 None:intergenic 45.0%
AGCCAGAGGAAACCATTGAA+GGG - chr5.3:73089451-73089470 MS.gene47716:CDS 45.0%
AGGATTTATTGCGGAGTTCC+TGG - chr5.3:73090387-73090406 MS.gene47716:CDS 45.0%
ATTTATTGCGGAGTTCCTGG+TGG - chr5.3:73090390-73090409 MS.gene47716:CDS 45.0%
CAACTTGGATGAAATGTGGG+GGG - chr5.3:73089677-73089696 MS.gene47716:CDS 45.0%
CAACTTTGACCATCGGATAG+CGG - chr5.3:73089714-73089733 MS.gene47716:CDS 45.0%
CAGGCAACTTGGATGAAATG+TGG - chr5.3:73089673-73089692 MS.gene47716:CDS 45.0%
CCAAGGCAATGCAAGAACAT+AGG - chr5.3:73089154-73089173 MS.gene47716:CDS 45.0%
CCAGAAGACACCACAAATGT+CGG + chr5.3:73090426-73090445 None:intergenic 45.0%
CCTATGTTCTTGCATTGCCT+TGG + chr5.3:73089157-73089176 None:intergenic 45.0%
CGCTCTGTAACCCAACATAA+TGG + chr5.3:73090273-73090292 None:intergenic 45.0%
CTACTTCCCCCTGTTAGTAT+TGG - chr5.3:73089201-73089220 MS.gene47716:CDS 45.0%
CTTGGGACTAATTGATTGGG+TGG - chr5.3:73090335-73090354 MS.gene47716:CDS 45.0%
GCAACTTGGATGAAATGTGG+GGG - chr5.3:73089676-73089695 MS.gene47716:CDS 45.0%
GGAATACTTGCACTTCATGG+AGG - chr5.3:73090599-73090618 MS.gene47716:CDS 45.0%
GGCAACTTGGATGAAATGTG+GGG - chr5.3:73089675-73089694 MS.gene47716:CDS 45.0%
GTCATAGAGTGAAGGATGGA+TGG + chr5.3:73089869-73089888 None:intergenic 45.0%
TAGAGTGAAGGATGGATGGA+AGG + chr5.3:73089865-73089884 None:intergenic 45.0%
TGCCAATGCCTAGTCCTAAA+AGG - chr5.3:73090541-73090560 MS.gene47716:CDS 45.0%
TGGAACAAGCTTCCCTTCAA+TGG + chr5.3:73089466-73089485 None:intergenic 45.0%
TGGCATGCAACTTTGACCAT+CGG - chr5.3:73089707-73089726 MS.gene47716:CDS 45.0%
TTCCCTTCAATGGTTTCCTC+TGG + chr5.3:73089456-73089475 None:intergenic 45.0%
! CCCCACAATAGTGTTATTGG+TGG + chr5.3:73089268-73089287 None:intergenic 45.0%
!! AGACTTGTTATTGCTCGAGC+GGG + chr5.3:73089785-73089804 None:intergenic 45.0%
AAGTAGCTCAGGTTCACCCA+TGG + chr5.3:73089361-73089380 None:intergenic 50.0%
ACTTTGCAACAACCGTGGTG+TGG - chr5.3:73089377-73089396 MS.gene47716:CDS 50.0%
AGCAGATGGACCGACATTTG+TGG - chr5.3:73090413-73090432 MS.gene47716:CDS 50.0%
AGCTGCAACTACCTCCTCTT+TGG + chr5.3:73089133-73089152 None:intergenic 50.0%
AGTGAAGGATGGATGGAAGG+TGG + chr5.3:73089862-73089881 None:intergenic 50.0%
CAATAAATCCTTGCCAGCCC+TGG + chr5.3:73090378-73090397 None:intergenic 50.0%
CCGACATTTGTGGTGTCTTC+TGG - chr5.3:73090423-73090442 MS.gene47716:CDS 50.0%
CGGTTGTTGCAAAGTAGCTC+AGG + chr5.3:73089372-73089391 None:intergenic 50.0%
GTGAAGGATGGATGGAAGGT+GGG + chr5.3:73089861-73089880 None:intergenic 50.0%
TCAGCCGCTTCAACCAATAG+TGG - chr5.3:73090096-73090115 MS.gene47716:intron 50.0%
TGGGTGTCTTGGGGTCAAAA+GGG + chr5.3:73089842-73089861 None:intergenic 50.0%
! ATTCCTTGTGGTGGTGATGC+TGG - chr5.3:73090510-73090529 MS.gene47716:CDS 50.0%
! GAAATGTGGGGGGTTAGTAG+TGG - chr5.3:73089687-73089706 MS.gene47716:CDS 50.0%
! GAGCTACTTTGCAACAACCG+TGG - chr5.3:73089372-73089391 MS.gene47716:CDS 50.0%
! GGGCTGGCAAGGATTTATTG+CGG - chr5.3:73090378-73090397 MS.gene47716:CDS 50.0%
!! ACATGGTGTGCTTGCTGTTC+AGG - chr5.3:73089494-73089513 MS.gene47716:CDS 50.0%
AATAGGCGTCCGCTATCCGA+TGG + chr5.3:73089726-73089745 None:intergenic 55.0%
GATGGAAGGTGGGTGTCTTG+GGG + chr5.3:73089851-73089870 None:intergenic 55.0%
GGAGGTAGTTGCAGCTACCA+AGG - chr5.3:73089137-73089156 MS.gene47716:CDS 55.0%
GGATGGAAGGTGGGTGTCTT+GGG + chr5.3:73089852-73089871 None:intergenic 55.0%
GTGGGTGTCTTGGGGTCAAA+AGG + chr5.3:73089843-73089862 None:intergenic 55.0%
TAGCCAGCATCACCACCACA+AGG + chr5.3:73090516-73090535 None:intergenic 55.0%
TGGAGGAGGTAGATCCGAGA+TGG + chr5.3:73089902-73089921 None:intergenic 55.0%
TGGATGGAAGGTGGGTGTCT+TGG + chr5.3:73089853-73089872 None:intergenic 55.0%
!! AAGAGCATCGACCGGTTCCA+GGG - chr5.3:73090358-73090377 MS.gene47716:CDS 55.0%
!! ATTGCTCGAGCGGGCTATCT+CGG + chr5.3:73089776-73089795 None:intergenic 55.0%
!! TCTGATTCGGGCTTGGGTTC+AGG + chr5.3:73089925-73089944 None:intergenic 55.0%
!! TTGGGTGGAAGAGCATCGAC+CGG - chr5.3:73090350-73090369 MS.gene47716:CDS 55.0%
ATCCTTGCCAGCCCTGGAAC+CGG + chr5.3:73090372-73090391 None:intergenic 60.0%
GATTCGGGCTTGGGTTCAGG+TGG + chr5.3:73089922-73089941 None:intergenic 60.0%
GGAGTTCCTGGTGGAGCAGA+TGG - chr5.3:73090399-73090418 MS.gene47716:CDS 60.0%
GGATGGGGGAAACCCGTAGT+TGG - chr5.3:73090477-73090496 MS.gene47716:CDS 60.0%
!! GAAGAGCATCGACCGGTTCC+AGG - chr5.3:73090357-73090376 MS.gene47716:CDS 60.0%
GGCTTGGGTTCAGGTGGAGG+AGG + chr5.3:73089916-73089935 None:intergenic 65.0%
GTCGGTCCATCTGCTCCACC+AGG + chr5.3:73090408-73090427 None:intergenic 65.0%
TCGGGCTTGGGTTCAGGTGG+AGG + chr5.3:73089919-73089938 None:intergenic 65.0%
! GACCGGTTCCAGGGCTGGCA+AGG - chr5.3:73090367-73090386 MS.gene47716:CDS 70.0%
! GCATCGACCGGTTCCAGGGC+TGG - chr5.3:73090362-73090381 MS.gene47716:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 73089093 73090674 73089093 ID=MS.gene47716
chr5.3 mRNA 73089093 73090674 73089093 ID=MS.gene47716.t1;Parent=MS.gene47716
chr5.3 exon 73090252 73090674 73090252 ID=MS.gene47716.t1.exon1;Parent=MS.gene47716.t1
chr5.3 CDS 73090252 73090674 73090252 ID=cds.MS.gene47716.t1;Parent=MS.gene47716.t1
chr5.3 exon 73089093 73090091 73089093 ID=MS.gene47716.t1.exon2;Parent=MS.gene47716.t1
chr5.3 CDS 73089093 73090091 73089093 ID=cds.MS.gene47716.t1;Parent=MS.gene47716.t1
Gene Sequence

>MS.gene47716

ATGAGTATTGGAAAAGTAGAATTCAGTGTGAATATTACCAAAGAGGAGGTAGTTGCAGCTACCAAGGCAATGCAAGAACATAGGCTAGCACTATCAAATCTTGATTTACTACTTCCCCCTGTTAGTATTGGTGTGTTCTTTTGCTACATGAATCCTATGATTACTATTGGCACCACCAATAACACTATTGTGGGGTGTTTGAAAAACTCCTTAGCTGAGGCTCTTGTTTCTTATTACGTCTTTGCTGGTGAAATGGTCACTAACTCCATGGGTGAACCTGAGCTACTTTGCAACAACCGTGGTGTGGATTTTGTTGAAGCTGTTGTTGATGTTGAGTTACAATCTCTCAATTTGCATAAGCCAGAGGAAACCATTGAAGGGAAGCTTGTTCCAAAGAAAACACATGGTGTGCTTGCTGTTCAGGTGAAAGTTTTTAATATTTGTTCATATTTACAAAAACATTTTGATCTCAAGGGAGTATTAGAATAGTGAAAGAATTATTATCATTATGCAGGTTTTTAGTTAATGATTAGTAGTAAGAAGCGTAGTAAGAAGTGTTTCAATTGTATGTATAATTGTGCAGGCAACTTGGATGAAATGTGGGGGGTTAGTAGTGGCATGCAACTTTGACCATCGGATAGCGGACGCCTATTCTACAAACATGTTTCTTGTATCATGGGCCGAGATAGCCCGCTCGAGCAATAACAAGTCTTTGATCTCAACAACACAACCTTGTTTTCAGAGGTCCCTTTTGACCCCAAGACACCCACCTTCCATCCATCCTTCACTCTATGACATGTACATCTCCATCTCGGATCTACCTCCTCCACCTGAACCCAAGCCCGAATCAGATATTAAAACAAACCAGCTAATAAGTCGCATGTACTATGTAACAAGCGAAGAACTCAACATCATGCAGTTACTAGCCAACACAAATAATAGTGGAAGCATTAAGCGCTCAAAACTTGAATCATTTTCTGCTTTCTTGTGGAAGATGGTTGCATCAGCCGCTTCAACCAATAGTGGAAATGTTGTTGCAAAAATGGGGATTGTGGTCGACGGAAGAATGAGATTAAGCAATGGTGACAAAAACAAAGAAGAGTTTATGAGTTCGTATTTTGGGAATGTGCTTTCAACTCCCTACAGTTGTAAATTAATAGAAGAACTTGTTGAAAAGCCATTATGTTGGGTTACAGAGCGAGTTCATGATTTTTTGGAGGCTGCGGACACGAAAGAACATTTCTTGGGACTAATTGATTGGGTGGAAGAGCATCGACCGGTTCCAGGGCTGGCAAGGATTTATTGCGGAGTTCCTGGTGGAGCAGATGGACCGACATTTGTGGTGTCTTCTGGACAAAGATTTCCAGAGTCAAAGGTAGATTTTGGATGGGGGAAACCCGTAGTTGGATCTTATCATATTCCTTGTGGTGGTGATGCTGGCTATGTTATGCCAATGCCTAGTCCTAAAAGGAATGGTGATTGGTTGATCTACATGAATCTTCCTAAGGAATACTTGCACTTCATGGAGGCTCAAGCTCCCCATTTCTTTAGACCAATCTCATGGGATTACCTTCTCAATTGA

Protein sequence

>MS.gene47716.t1

MSIGKVEFSVNITKEEVVAATKAMQEHRLALSNLDLLLPPVSIGVFFCYMNPMITIGTTNNTIVGCLKNSLAEALVSYYVFAGEMVTNSMGEPELLCNNRGVDFVEAVVDVELQSLNLHKPEETIEGKLVPKKTHGVLAVQATWMKCGGLVVACNFDHRIADAYSTNMFLVSWAEIARSSNNKSLISTTQPCFQRSLLTPRHPPSIHPSLYDMYISISDLPPPPEPKPESDIKTNQLISRMYYVTSEELNIMQLLANTNNSGSIKRSKLESFSAFLWKMVASAASTNSGNVVAKMGIVVDGRMRLSNGDKNKEEFMSSYFGNVLSTPYSCKLIEELVEKPLCWVTERVHDFLEAADTKEHFLGLIDWVEEHRPVPGLARIYCGVPGGADGPTFVVSSGQRFPESKVDFGWGKPVVGSYHIPCGGDAGYVMPMPSPKRNGDWLIYMNLPKEYLHFMEAQAPHFFRPISWDYLLN