AlfalfaGEDB | MS.gene48021

M. sativa cultivar XinJiangDaYe / MS.gene48021

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48021.t1	XP_003617368.1	93.4	473	28	2	1	472	1	471	5.90E-265	922.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48021.t1	Q9FI78	25.1	459	301	16	8	455	1	427	6.6e-31	136.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48021.t1	G7K3A2	93.4	473	28	2	1	472	1	471	4.2e-265	922.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049549	MS.gene48021	0.826396	2.78E-54	-1.69E-46
MS.gene052211	MS.gene48021	0.842321	2.78E-58	-1.69E-46
MS.gene052212	MS.gene48021	0.815312	9.86E-52	-1.69E-46
MS.gene05240	MS.gene48021	0.80387	2.84E-49	-1.69E-46
MS.gene053406	MS.gene48021	0.8001	1.69E-48	-1.69E-46
MS.gene054146	MS.gene48021	0.832156	1.11E-55	-1.69E-46
MS.gene055240	MS.gene48021	0.81934	1.22E-52	-1.69E-46
MS.gene055977	MS.gene48021	0.801023	1.09E-48	-1.69E-46
MS.gene056748	MS.gene48021	0.80593	1.05E-49	-1.69E-46
MS.gene058603	MS.gene48021	0.816044	6.78E-52	-1.69E-46
MS.gene059060	MS.gene48021	0.803118	4.06E-49	-1.69E-46
MS.gene059265	MS.gene48021	0.810868	9.31E-51	-1.69E-46
MS.gene060087	MS.gene48021	0.806911	6.54E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene48021	MS.gene038274	PPI
MS.gene48021	MS.gene38361	PPI
MS.gene017058	MS.gene48021	PPI
MS.gene48021	MS.gene38363	PPI
MS.gene48021	MS.gene017058	PPI
MS.gene48021	MS.gene32936	PPI
MS.gene48021	MS.gene38359	PPI
MS.gene48021	MS.gene039597	PPI
MS.gene48021	MS.gene38700	PPI
MS.gene48021	MS.gene019178	PPI
MS.gene48021	MS.gene32933	PPI
MS.gene48021	MS.gene028134	PPI
MS.gene72785	MS.gene48021	PPI
MS.gene48021	MS.gene47721	PPI
MS.gene48021	MS.gene38706	PPI
MS.gene48021	MS.gene48023	PPI
MS.gene48023	MS.gene48021	PPI
MS.gene48021	MS.gene38701	PPI
MS.gene48021	MS.gene69496	PPI
MS.gene48021	MS.gene017054	PPI
MS.gene48021	MS.gene012960	PPI
MS.gene48021	MS.gene012957	PPI
MS.gene48021	MS.gene38699	PPI
MS.gene48021	MS.gene83461	PPI
MS.gene48021	MS.gene95242	PPI
MS.gene48021	MS.gene038271	PPI
MS.gene48021	MS.gene38704	PPI
MS.gene48021	MS.gene012959	PPI
MS.gene48021	MS.gene48027	PPI
MS.gene48021	MS.gene22199	PPI
MS.gene48021	MS.gene47719	PPI
MS.gene48021	MS.gene82145	PPI
MS.gene48021	MS.gene32938	PPI
MS.gene48021	MS.gene069321	PPI
MS.gene48021	MS.gene000507	PPI
MS.gene48021	MS.gene38702	PPI
MS.gene48021	MS.gene32934	PPI
MS.gene48021	MS.gene012965	PPI
MS.gene48021	MS.gene046246	PPI
MS.gene48021	MS.gene38703	PPI
MS.gene48021	MS.gene38365	PPI
MS.gene48021	MS.gene017053	PPI
MS.gene48021	MS.gene47716	PPI
MS.gene48021	MS.gene012961	PPI
MS.gene48021	MS.gene38362	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48021.t1	MTR_5g090830	93.446	473	28	2	1	472	1	471	0.0	919
MS.gene48021.t1	MTR_5g090810	92.178	473	34	2	1	472	1	471	0.0	909
MS.gene48021.t1	MTR_5g090880	80.127	473	93	1	1	472	1	473	0.0	779
MS.gene48021.t1	MTR_3g450710	67.872	470	130	7	10	470	11	468	0.0	627
MS.gene48021.t1	MTR_3g450690	64.894	470	148	5	10	470	11	472	0.0	611
MS.gene48021.t1	MTR_5g026320	36.627	415	235	11	29	440	39	428	4.42e-72	235
MS.gene48021.t1	MTR_8g096530	27.706	462	285	18	7	458	4	426	2.49e-33	131
MS.gene48021.t1	MTR_8g075610	25.287	435	278	14	32	455	16	414	2.83e-33	130
MS.gene48021.t1	MTR_2g064495	25.054	463	291	17	10	455	3	426	8.17e-32	126
MS.gene48021.t1	MTR_2g105330	25.054	463	291	17	10	455	3	426	3.42e-31	125
MS.gene48021.t1	MTR_4g007540	23.913	414	278	12	10	414	3	388	1.45e-29	120
MS.gene48021.t1	MTR_4g097090	25.216	464	300	15	23	465	19	456	4.73e-28	116
MS.gene48021.t1	MTR_7g086000	29.301	372	215	15	66	412	60	408	9.57e-28	115
MS.gene48021.t1	MTR_3g049150	25.740	439	273	18	31	446	23	431	1.17e-27	115
MS.gene48021.t1	MTR_3g098980	26.854	391	242	14	31	413	35	389	1.59e-27	114
MS.gene48021.t1	MTR_4g127650	23.881	469	299	14	23	465	20	456	2.15e-27	114
MS.gene48021.t1	MTR_1g040215	26.617	402	232	14	63	436	61	427	7.89e-26	110
MS.gene48021.t1	MTR_5g076700	25.063	399	252	13	63	447	62	427	1.25e-25	109
MS.gene48021.t1	MTR_7g114470	25.592	422	264	16	8	415	9	394	2.15e-25	108
MS.gene48021.t1	MTR_7g114520	25.490	459	283	17	8	446	9	428	4.78e-25	107
MS.gene48021.t1	MTR_7g027175	25.416	421	268	15	4	414	9	393	1.07e-24	106
MS.gene48021.t1	MTR_7g114560	23.929	397	258	11	63	447	62	426	1.30e-24	106
MS.gene48021.t1	MTR_1g053315	25.061	407	251	15	29	415	40	412	3.25e-23	102
MS.gene48021.t1	MTR_1g081660	26.005	373	230	15	63	424	70	407	3.26e-23	102
MS.gene48021.t1	MTR_1g040175	25.062	403	237	15	63	436	61	427	6.68e-23	101
MS.gene48021.t1	MTR_7g114550	24.359	468	291	16	1	447	1	426	7.39e-23	101
MS.gene48021.t1	MTR_7g114490	24.029	412	261	13	63	458	62	437	2.21e-22	100
MS.gene48021.t1	MTR_3g026950	24.312	436	287	13	31	446	23	435	2.49e-22	99.8
MS.gene48021.t1	MTR_7g085930	27.848	395	233	19	66	429	59	432	7.92e-22	98.2
MS.gene48021.t1	MTR_7g085990	25.916	382	227	17	66	414	55	413	1.46e-21	97.4
MS.gene48021.t1	MTR_2g015430	24.129	402	258	17	24	413	18	384	2.81e-18	87.4
MS.gene48021.t1	MTR_7g084940	25.694	432	249	18	6	414	12	394	1.01e-17	85.9
MS.gene48021.t1	MTR_4g055170	23.956	455	298	18	17	451	19	445	5.44e-17	83.6
MS.gene48021.t1	MTR_1g040195	24.523	367	216	14	96	436	71	402	1.26e-16	82.4
MS.gene48021.t1	MTR_3g086020	23.908	435	254	20	6	426	1	372	1.50e-16	82.0
MS.gene48021.t1	MTR_5g084870	23.040	421	266	16	8	414	1	377	3.84e-16	80.9
MS.gene48021.t1	MTR_3g070170	23.514	387	236	13	60	413	60	419	1.01e-15	79.7
MS.gene48021.t1	MTR_7g084910	25.435	460	256	19	7	434	14	418	1.75e-15	79.0
MS.gene48021.t1	MTR_6g053810	26.518	313	196	11	66	364	71	363	2.73e-15	78.2
MS.gene48021.t1	MTR_3g101960	23.292	322	198	12	49	348	48	342	5.04e-15	77.4
MS.gene48021.t1	MTR_3g101930	23.181	371	233	11	61	411	65	403	7.92e-15	77.0
MS.gene48021.t1	MTR_3g112180	22.143	420	250	15	18	411	20	388	1.60e-14	75.9
MS.gene48021.t1	MTR_4g094842	24.468	376	217	13	66	413	62	398	4.86e-14	74.3
MS.gene48021.t1	MTR_5g084850	22.695	423	264	14	8	414	1	376	7.51e-13	70.5
MS.gene48021.t1	MTR_7g110610	28.947	152	93	3	66	202	55	206	1.32e-12	69.7
MS.gene48021.t1	MTR_3g088420	24.060	399	237	16	36	413	32	385	1.69e-12	69.7
MS.gene48021.t1	MTR_1g040320	30.464	151	93	4	63	205	61	207	3.59e-12	68.6
MS.gene48021.t1	MTR_8g041770	23.971	413	249	15	23	413	22	391	4.87e-12	68.2
MS.gene48021.t1	MTR_4g094828	23.325	403	244	16	36	413	37	399	6.95e-12	67.8
MS.gene48021.t1	MTR_3g071320	23.901	364	231	13	66	414	60	392	2.39e-11	65.9
MS.gene48021.t1	MTR_3g465880	24.242	396	245	15	1	369	1	368	9.86e-11	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48021.t1	AT1G32910	58.263	472	164	11	8	470	11	458	1.02e-178	509
MS.gene48021.t1	AT1G78990	56.745	467	174	9	8	470	11	453	5.32e-178	506
MS.gene48021.t1	AT5G16410	51.368	475	190	14	8	470	34	479	7.70e-158	456
MS.gene48021.t1	AT1G31490	36.652	442	238	12	8	440	4	412	5.12e-77	248
MS.gene48021.t1	AT5G48930	25.163	461	298	17	8	455	1	427	3.87e-36	139
MS.gene48021.t1	AT2G19070	24.034	466	310	16	18	465	12	451	4.09e-27	113
MS.gene48021.t1	AT5G41040	25.127	394	250	13	31	414	38	396	2.47e-26	111
MS.gene48021.t1	AT5G57840	26.068	468	281	18	8	451	1	427	3.02e-26	111
MS.gene48021.t1	AT5G41040	25.127	394	250	13	31	414	54	412	3.21e-26	111
MS.gene48021.t1	AT3G48720	27.089	395	239	13	31	414	30	386	1.53e-24	105
MS.gene48021.t1	AT3G03480	25.647	464	270	19	6	436	15	436	1.92e-24	105
MS.gene48021.t1	AT5G17540	24.786	468	265	19	4	439	3	415	5.26e-23	101
MS.gene48021.t1	AT1G27620	22.195	401	265	13	66	455	58	422	6.50e-21	95.5
MS.gene48021.t1	AT3G50270	27.394	376	218	16	60	413	56	398	1.80e-20	94.0
MS.gene48021.t1	AT5G63560	25.490	408	245	14	19	413	20	381	3.00e-20	93.2
MS.gene48021.t1	AT3G50280	24.766	428	262	18	9	413	3	393	1.35e-19	91.3
MS.gene48021.t1	AT1G03390	24.816	407	252	17	27	415	39	409	2.04e-19	90.9
MS.gene48021.t1	AT1G65450	26.617	402	240	16	31	415	40	403	1.35e-18	88.6
MS.gene48021.t1	AT1G24430	25.236	424	249	18	8	413	3	376	2.25e-18	87.8
MS.gene48021.t1	AT1G28680	25.537	419	263	19	12	413	1	387	4.86e-18	86.7
MS.gene48021.t1	AT3G30280	25.513	439	265	14	8	431	4	395	1.07e-16	82.8
MS.gene48021.t1	AT5G07080	24.185	368	237	10	60	413	64	403	3.32e-15	78.2
MS.gene48021.t1	AT5G47950	29.944	177	110	4	8	177	4	173	5.71e-14	73.9
MS.gene48021.t1	AT5G01210	25.131	382	201	15	79	413	75	418	6.77e-14	73.9
MS.gene48021.t1	AT3G50300	24.397	373	225	15	63	413	56	393	1.19e-13	73.2
MS.gene48021.t1	AT4G15390	22.459	423	274	14	8	414	6	390	1.30e-13	73.2
MS.gene48021.t1	AT4G15390	22.459	423	274	14	8	414	6	390	1.30e-13	73.2
MS.gene48021.t1	AT2G25150	22.192	365	242	11	63	413	64	400	2.26e-13	72.4
MS.gene48021.t1	AT5G07850	24.044	366	227	14	66	413	70	402	3.15e-13	72.0
MS.gene48021.t1	AT1G24420	22.449	441	263	17	6	419	1	389	3.28e-13	71.6
MS.gene48021.t1	AT1G24420	22.449	441	263	17	6	419	1	389	3.28e-13	71.6
MS.gene48021.t1	AT5G42830	24.044	366	229	14	66	413	64	398	4.15e-13	71.6
MS.gene48021.t1	AT5G67160	24.480	433	255	19	59	472	49	428	5.37e-13	71.2

Find 129 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGATTATGAATTCATATTT+TGG	0.139298	5.2:-76845289	MS.gene48021:CDS
TTCTGCTTTCTTGTGGAAAA+TGG	0.174817	5.2:-76845413	MS.gene48021:CDS
CCAGAGTCGAAGGTTGATTT+TGG	0.189640	5.2:-76845025	MS.gene48021:CDS
TAGTATATGCAACTTATTAT+TGG	0.235744	5.2:+76845523	None:intergenic
AGCACACCATGTTTCTTCTT+TGG	0.268793	5.2:+76846143	None:intergenic
GTTTCTTATTATGCCTTTGC+TGG	0.282844	5.2:-76846308	MS.gene48021:CDS
TTAATATCAGAGTCAAATTC+AGG	0.299615	5.2:+76845553	None:intergenic
ACATGGTGTGCTTGCTGTTC+AGG	0.312822	5.2:-76846132	MS.gene48021:intron
AGTATATGCAACTTATTATT+GGG	0.316657	5.2:+76845524	None:intergenic
GGATGGAAGGTGGGCGTCGT+GGG	0.321410	5.2:+76845632	None:intergenic
AAGAACACACCGACATCAAC+AGG	0.337593	5.2:+76846413	None:intergenic
ATCATTGGGCTGAGCCATTT+CGG	0.339987	5.2:+76845708	None:intergenic
TAAGGGACACTTGCACTTCA+TGG	0.343370	5.2:-76844885	MS.gene48021:CDS
GGTGACAGAGGAGCATTTCT+TGG	0.346724	5.2:-76845164	MS.gene48021:CDS
ATTTCTTGGGTTTAATAGAT+TGG	0.349608	5.2:-76845150	MS.gene48021:CDS
GAAGAGCATCGTCCAGTTCC+TGG	0.354253	5.2:-76845124	MS.gene48021:CDS
TGGATGGAAGGTGGGCGTCG+TGG	0.357861	5.2:+76845631	None:intergenic
TGCCAATGCCAAGCCCTAAA+AGG	0.358606	5.2:-76844940	MS.gene48021:CDS
TTTCCTTGGGGAGGTAGTGC+TGG	0.358995	5.2:-76844971	MS.gene48021:CDS
AAGGAACATGTTTGCAGAAT+AGG	0.360809	5.2:+76845741	None:intergenic
TTCCCTTCTACAGTTTCATC+TGG	0.371632	5.2:+76846173	None:intergenic
CGAAAACAAGGTTGCGTTGT+TGG	0.373331	5.2:+76845670	None:intergenic
ATTTCTTTAGACCAGTCTCT+TGG	0.380544	5.2:-76844847	MS.gene48021:CDS
GATCAAAGACTTGTTATCAT+TGG	0.392905	5.2:+76845693	None:intergenic
TTTCTTGGGTTTAATAGATT+GGG	0.393187	5.2:-76845149	MS.gene48021:CDS
CCAAAATCAACCTTCGACTC+TGG	0.403209	5.2:+76845025	None:intergenic
ATCAAAGACTTGTTATCATT+GGG	0.404681	5.2:+76845694	None:intergenic
TCAGAGTCAAATTCAGGTTC+AGG	0.411350	5.2:+76845559	None:intergenic
CAAACATGTTCCTTGTATCA+TGG	0.413069	5.2:-76845732	MS.gene48021:CDS
CTAATCGCTAAAATGTGTAT+TGG	0.419429	5.2:-76846542	None:intergenic
TTTCTTTAGACCAGTCTCTT+GGG	0.423523	5.2:-76844846	MS.gene48021:CDS
ATCCTTGCCAATCCAGGAAC+TGG	0.426912	5.2:+76845112	None:intergenic
CCTTGTATCATGGGCCGAAA+TGG	0.428090	5.2:-76845722	MS.gene48021:CDS
CCAACATTTGTGGTATCTTC+AGG	0.429306	5.2:-76845058	MS.gene48021:CDS
ATTAAATCAAGGTTTGATAG+TGG	0.431310	5.2:+76846443	None:intergenic
GAAAACAAGGTTGCGTTGTT+GGG	0.433945	5.2:+76845671	None:intergenic
AATCCAGATGAAACTGTAGA+AGG	0.442984	5.2:-76846176	MS.gene48021:CDS
GTGACAGAGGAGCATTTCTT+GGG	0.453426	5.2:-76845163	MS.gene48021:CDS
ATGAAGTGCAAGTGTCCCTT+AGG	0.455932	5.2:+76844887	None:intergenic
TTTCCACAAGAAAGCAGAAA+AGG	0.464789	5.2:+76845417	None:intergenic
CGCTACCAATGCAAGAGCAT+TGG	0.468968	5.2:-76846469	MS.gene48021:CDS
GCTCTACATGCACCTTCCTA+AGG	0.469205	5.2:-76844903	MS.gene48021:CDS
GTCTAAAGAAATGAGGAGCC+TGG	0.472034	5.2:+76844858	None:intergenic
TGGGTGCCAGTAGTAATTAT+AGG	0.473421	5.2:+76846377	None:intergenic
TATGACCAACGAGGAAATAG+TGG	0.494458	5.2:-76846498	MS.gene48021:CDS
AGCTGCCACTATTTCCTCGT+TGG	0.494647	5.2:+76846493	None:intergenic
CAATAAATCCTTGCCAATCC+AGG	0.495236	5.2:+76845106	None:intergenic
GATGAAATGTGGTGGATTAG+TGG	0.498028	5.2:-76845797	MS.gene48021:CDS
GGCACCCACCATAGCATTGT+GGG	0.501789	5.2:-76846362	MS.gene48021:CDS
AGGATTTATTGTGGTAGCAC+TGG	0.506494	5.2:-76845094	MS.gene48021:CDS
GGTTGATAATCCGCTATGTT+GGG	0.513429	5.2:-76845221	MS.gene48021:CDS
TCAAATTCAGGTTCAGGTGG+TGG	0.515057	5.2:+76845565	None:intergenic
CTTGCACTTCATGGAGGCCC+AGG	0.516007	5.2:-76844876	MS.gene48021:CDS
CCATTTCGGCCCATGATACA+AGG	0.516374	5.2:+76845722	None:intergenic
ACTACCACCATTGTTTGAGT+TGG	0.517260	5.2:+76845462	None:intergenic
GTGAAGGATGGATGGAAGGT+GGG	0.517707	5.2:+76845623	None:intergenic
GCATCGTCCAGTTCCTGGAT+TGG	0.519656	5.2:-76845119	MS.gene48021:CDS
TCAAACATCCCACAATGCTA+TGG	0.522391	5.2:+76846354	None:intergenic
GTCATAAAGTGAAGGATGGA+TGG	0.526884	5.2:+76845615	None:intergenic
TTTGTTAATCTACCACCATA+GGG	0.527591	5.2:+76845250	None:intergenic
TCACTAGCCAACTCAAACAA+TGG	0.529140	5.2:-76845469	MS.gene48021:CDS
GGTAGCACTGGTGGAGAAGA+AGG	0.530503	5.2:-76845082	MS.gene48021:CDS
TTTCTTGTGGAAAATGGTTG+CGG	0.534078	5.2:-76845407	MS.gene48021:CDS
ATCCAGATGAAACTGTAGAA+GGG	0.535674	5.2:-76846175	MS.gene48021:CDS
AGTGAAGGATGGATGGAAGG+TGG	0.539276	5.2:+76845622	None:intergenic
ATTTATTGTGGTAGCACTGG+TGG	0.539802	5.2:-76845091	MS.gene48021:CDS
AAACATGTTCCTTGTATCAT+GGG	0.539896	5.2:-76845731	MS.gene48021:CDS
TGGTTGATAATCCGCTATGT+TGG	0.541576	5.2:-76845222	MS.gene48021:CDS
AGAAGAAGGACCAACATTTG+TGG	0.543509	5.2:-76845068	MS.gene48021:CDS
TGAAGTCGTTGCTAATTCCA+TGG	0.543846	5.2:-76846285	MS.gene48021:CDS
ACAAACACTAATATTAATTG+AGG	0.550824	5.2:+76844816	None:intergenic
AATTCAGGTTCAGGTGGTGG+AGG	0.553611	5.2:+76845568	None:intergenic
AATAAGCCTATAATTACTAC+TGG	0.553926	5.2:-76846383	MS.gene48021:CDS
TTTGGAGGCGGCGGTGACAG+AGG	0.555868	5.2:-76845176	MS.gene48021:CDS
GCCCTAAAAGGAATGGTGAC+TGG	0.557544	5.2:-76844928	MS.gene48021:CDS
AGGAGGTAATCCCAAGAGAC+TGG	0.562245	5.2:+76844836	None:intergenic
TGGTAGATTAACAAAAGAGC+TGG	0.565630	5.2:-76845242	MS.gene48021:CDS
AACATCCCACAATGCTATGG+TGG	0.566613	5.2:+76846357	None:intergenic
CTTGGGTTTAATAGATTGGG+TGG	0.568431	5.2:-76845146	MS.gene48021:CDS
CGATTGTTGCAAAGTAACTC+AGG	0.570367	5.2:+76846257	None:intergenic
TCTAAAGAAATGAGGAGCCT+GGG	0.578551	5.2:+76844859	None:intergenic
CCTGAAGATACCACAAATGT+TGG	0.579526	5.2:+76845058	None:intergenic
GATGGAAGGTGGGCGTCGTG+GGG	0.580404	5.2:+76845633	None:intergenic
AGAAGTGACTGTCGAAAACA+AGG	0.582200	5.2:+76845658	None:intergenic
GGAAGCCAATGCTCTTGCAT+TGG	0.585364	5.2:+76846464	None:intergenic
ACAAAGGTTTCCAGAGTCGA+AGG	0.585762	5.2:-76845035	MS.gene48021:CDS
GGTGGAGGTAGATCAGAGAC+GGG	0.587879	5.2:+76845583	None:intergenic
AGCAACGACTTCACCAGCAA+AGG	0.588840	5.2:+76846295	None:intergenic
TAAAGTGAAGGATGGATGGA+AGG	0.590822	5.2:+76845619	None:intergenic
TTGTGGTATCTTCAGGACAA+AGG	0.593564	5.2:-76845051	MS.gene48021:CDS
ACATGTCATAAAGTGAAGGA+TGG	0.594714	5.2:+76845611	None:intergenic
ATGCCAAGCCCTAAAAGGAA+TGG	0.595010	5.2:-76844935	MS.gene48021:CDS
TGGTGGAGGTAGATCAGAGA+CGG	0.600538	5.2:+76845582	None:intergenic
CTCTACATGCACCTTCCTAA+GGG	0.601014	5.2:-76844902	MS.gene48021:CDS
AGTGCAAGTGTCCCTTAGGA+AGG	0.602305	5.2:+76844891	None:intergenic
AACACTAATATTAATTGAGG+AGG	0.603374	5.2:+76844819	None:intergenic
TTGTGGTTGACGGAAGAAAG+AGG	0.605169	5.2:-76845339	MS.gene48021:CDS
GAGTTACTTTGCAACAATCG+TGG	0.605999	5.2:-76846254	MS.gene48021:CDS
GTCCAGTTCCTGGATTGGCA+AGG	0.607030	5.2:-76845114	MS.gene48021:CDS
TTGTTCTTGTGCAGACAACA+TGG	0.607336	5.2:-76845819	MS.gene48021:intron
AATGTGCTTTCGATTCCCTA+TGG	0.610089	5.2:-76845265	MS.gene48021:CDS
GAGACTGGTCTAAAGAAATG+AGG	0.612013	5.2:+76844851	None:intergenic
AGAACACACCGACATCAACA+GGG	0.612828	5.2:+76846414	None:intergenic
AAGTAACTCAGGTTCACCCA+TGG	0.618644	5.2:+76846268	None:intergenic
GTGCTTTCGATTCCCTATGG+TGG	0.619185	5.2:-76845262	MS.gene48021:CDS
AAAATGGGGCTTGTGGTTGA+CGG	0.619932	5.2:-76845349	MS.gene48021:CDS
CTTGTTCCAAAGAAGAAACA+TGG	0.620389	5.2:-76846149	MS.gene48021:CDS
TGGCACCCACCATAGCATTG+TGG	0.626128	5.2:-76846363	MS.gene48021:CDS
ACATCCCACAATGCTATGGT+GGG	0.631579	5.2:+76846358	None:intergenic
ACATACATGTCATAAAGTGA+AGG	0.639724	5.2:+76845607	None:intergenic
ACTTTGCAACAATCGTGGAG+TGG	0.644603	5.2:-76846249	MS.gene48021:CDS
GAAGTCGTTGCTAATTCCAT+GGG	0.644829	5.2:-76846284	MS.gene48021:CDS
GAACACACCGACATCAACAG+GGG	0.646980	5.2:+76846415	None:intergenic
GGATTGGCAAGGATTTATTG+TGG	0.648587	5.2:-76845103	MS.gene48021:CDS
GAGTCAAATTCAGGTTCAGG+TGG	0.648966	5.2:+76845562	None:intergenic
AAATGATAAGATCCAAACAC+AGG	0.657847	5.2:+76844992	None:intergenic
TGATAATCCGCTATGTTGGG+TGG	0.663374	5.2:-76845218	MS.gene48021:CDS
ACAACATGGATGAAATGTGG+TGG	0.668180	5.2:-76845805	MS.gene48021:CDS
AATAGGCATCCGCTATACGA+TGG	0.674136	5.2:+76845758	None:intergenic
GAAATGTGGTGGATTAGTGG+TGG	0.677628	5.2:-76845794	MS.gene48021:CDS
AACACACCGACATCAACAGG+GGG	0.687180	5.2:+76846416	None:intergenic
CAGACAACATGGATGAAATG+TGG	0.692012	5.2:-76845808	MS.gene48021:CDS
CTAGCCAACTCAAACAATGG+TGG	0.694488	5.2:-76845466	MS.gene48021:CDS
TAGCCAGCACTACCTCCCCA+AGG	0.698597	5.2:+76844968	None:intergenic
GGGACACTTGCACTTCATGG+AGG	0.714604	5.2:-76844882	MS.gene48021:CDS
CGATCTGCCACCCAACATAG+CGG	0.715687	5.2:+76845211	None:intergenic
GGAAGAAAGAGGCTAAGCAA+CGG	0.723389	5.2:-76845328	MS.gene48021:CDS
CAACGTGAATATGACCAACG+AGG	0.763069	5.2:-76846507	MS.gene48021:CDS
GTGGAGGTAGATCAGAGACG+GGG	0.788848	5.2:+76845584	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTATATGCAACTTATTATT+GGG	+	chr5.2:76845838-76845857	None:intergenic	20.0%
!!	TAGTATATGCAACTTATTAT+TGG	+	chr5.2:76845839-76845858	None:intergenic	20.0%
!!	TTTGAAAAATTCTTTAGCTA+AGG	-	chr5.2:76845023-76845042	MS.gene48021:CDS	20.0%
!!!	CGATTATGAATTCATATTTT+GGG	-	chr5.2:76846071-76846090	MS.gene48021:intron	20.0%
!	AAAACATTGTTGCAAAAATG+GGG	-	chr5.2:76845996-76846015	MS.gene48021:intron	25.0%
!	AATAAGCCTATAATTACTAC+TGG	-	chr5.2:76844976-76844995	MS.gene48021:CDS	25.0%
!	AGTAAAATGATAAGACCAAT+GGG	+	chr5.2:76845390-76845409	None:intergenic	25.0%
!	ATTAAATCAAGGTTTGATAG+TGG	+	chr5.2:76844919-76844938	None:intergenic	25.0%
!	ATTTCTTGGGTTTAATAGAT+TGG	-	chr5.2:76846209-76846228	MS.gene48021:CDS	25.0%
!	GAAAACATTGTTGCAAAAAT+GGG	-	chr5.2:76845995-76846014	MS.gene48021:intron	25.0%
!	TATAATCTATACCATATGCA+AGG	+	chr5.2:76845503-76845522	None:intergenic	25.0%
!	TGAAAACATTGTTGCAAAAA+TGG	-	chr5.2:76845994-76846013	MS.gene48021:intron	25.0%
!	TTAATATCAGAGTCAAATTC+AGG	+	chr5.2:76845809-76845828	None:intergenic	25.0%
!	TTTCTTGGGTTTAATAGATT+GGG	-	chr5.2:76846210-76846229	MS.gene48021:CDS	25.0%
!!	GCGATTATGAATTCATATTT+TGG	-	chr5.2:76846070-76846089	MS.gene48021:intron	25.0%
!!!	AATTTTACTTTTGAACCACT+TGG	-	chr5.2:76845449-76845468	MS.gene48021:CDS	25.0%
!!!	ATCAAAGACTTGTTATCATT+GGG	+	chr5.2:76845668-76845687	None:intergenic	25.0%
	AAACATGTTCCTTGTATCAT+GGG	-	chr5.2:76845628-76845647	MS.gene48021:CDS	30.0%
	AAATGATAAGATCCAAACAC+AGG	+	chr5.2:76846370-76846389	None:intergenic	30.0%
	ACATACATGTCATAAAGTGA+AGG	+	chr5.2:76845755-76845774	None:intergenic	30.0%
	ATTAGTACTTGTGTTTAGAC+AGG	-	chr5.2:76845355-76845374	MS.gene48021:CDS	30.0%
	CAGTAAAATGATAAGACCAA+TGG	+	chr5.2:76845391-76845410	None:intergenic	30.0%
	TTTGTTAATCTACCACCATA+GGG	+	chr5.2:76846112-76846131	None:intergenic	30.0%
!	ACCAAAACATTTTGTTCTCA+AGG	-	chr5.2:76845271-76845290	MS.gene48021:CDS	30.0%
!	CCAAAACATTTTGTTCTCAA+GGG	-	chr5.2:76845272-76845291	MS.gene48021:CDS	30.0%
!	TGGATCTTATCATTTTCCTT+GGG	-	chr5.2:76846375-76846394	MS.gene48021:CDS	30.0%
!	TTGGATCTTATCATTTTCCT+TGG	-	chr5.2:76846374-76846393	MS.gene48021:CDS	30.0%
!	TTTTGTTAATCTACCACCAT+AGG	+	chr5.2:76846113-76846132	None:intergenic	30.0%
!!	GATCAAAGACTTGTTATCAT+TGG	+	chr5.2:76845669-76845688	None:intergenic	30.0%
!!	GTTTTCATTATCGATTGAAG+CGG	+	chr5.2:76845981-76846000	None:intergenic	30.0%
	AAGGAACATGTTTGCAGAAT+AGG	+	chr5.2:76845621-76845640	None:intergenic	35.0%
	AATCCAGATGAAACTGTAGA+AGG	-	chr5.2:76845183-76845202	MS.gene48021:CDS	35.0%
	AATCTATACCATATGCAAGG+TGG	+	chr5.2:76845500-76845519	None:intergenic	35.0%
	ACATGTCATAAAGTGAAGGA+TGG	+	chr5.2:76845751-76845770	None:intergenic	35.0%
	ATCCAGATGAAACTGTAGAA+GGG	-	chr5.2:76845184-76845203	MS.gene48021:CDS	35.0%
	ATTTCTTTAGACCAGTCTCT+TGG	-	chr5.2:76846512-76846531	MS.gene48021:CDS	35.0%
	CAAACATGTTCCTTGTATCA+TGG	-	chr5.2:76845627-76845646	MS.gene48021:CDS	35.0%
	CTTGTTCCAAAGAAGAAACA+TGG	-	chr5.2:76845210-76845229	MS.gene48021:CDS	35.0%
	GTTTCTTATTATGCCTTTGC+TGG	-	chr5.2:76845051-76845070	MS.gene48021:CDS	35.0%
	TCTCAAGGGAATATTCGAAT+AGG	-	chr5.2:76845286-76845305	MS.gene48021:CDS	35.0%
	TGGTAGATTAACAAAAGAGC+TGG	-	chr5.2:76846117-76846136	MS.gene48021:intron	35.0%
	TTCTGCTTTCTTGTGGAAAA+TGG	-	chr5.2:76845946-76845965	MS.gene48021:intron	35.0%
	TTTCCACAAGAAAGCAGAAA+AGG	+	chr5.2:76845945-76845964	None:intergenic	35.0%
	TTTCTTGTGGAAAATGGTTG+CGG	-	chr5.2:76845952-76845971	MS.gene48021:intron	35.0%
	TTTCTTTAGACCAGTCTCTT+GGG	-	chr5.2:76846513-76846532	MS.gene48021:CDS	35.0%
!	AGATCGAGTTCATGAGTTTT+TGG	-	chr5.2:76846165-76846184	MS.gene48021:CDS	35.0%
!	CCCTTGAGAACAAAATGTTT+TGG	+	chr5.2:76845275-76845294	None:intergenic	35.0%
!	GGATCTTATCATTTTCCTTG+GGG	-	chr5.2:76846376-76846395	MS.gene48021:CDS	35.0%
!!	TTACTTTTGAACCACTTGGT+TGG	-	chr5.2:76845453-76845472	MS.gene48021:CDS	35.0%
!!!	TGGTTTAGTGGTGTTGTTTT+GGG	-	chr5.2:76845473-76845492	MS.gene48021:CDS	35.0%
!!!	TTGGTTTAGTGGTGTTGTTT+TGG	-	chr5.2:76845472-76845491	MS.gene48021:CDS	35.0%
	AATGTGCTTTCGATTCCCTA+TGG	-	chr5.2:76846094-76846113	MS.gene48021:intron	40.0%
	ACAACATGGATGAAATGTGG+TGG	-	chr5.2:76845554-76845573	MS.gene48021:CDS	40.0%
	ACTACCACCATTGTTTGAGT+TGG	+	chr5.2:76845900-76845919	None:intergenic	40.0%
	AGAAGAAGGACCAACATTTG+TGG	-	chr5.2:76846291-76846310	MS.gene48021:CDS	40.0%
	AGAAGTGACTGTCGAAAACA+AGG	+	chr5.2:76845704-76845723	None:intergenic	40.0%
	AGCACACCATGTTTCTTCTT+TGG	+	chr5.2:76845219-76845238	None:intergenic	40.0%
	AGGATTTATTGTGGTAGCAC+TGG	-	chr5.2:76846265-76846284	MS.gene48021:CDS	40.0%
	CAATAAATCCTTGCCAATCC+AGG	+	chr5.2:76846256-76846275	None:intergenic	40.0%
	CAGACAACATGGATGAAATG+TGG	-	chr5.2:76845551-76845570	MS.gene48021:CDS	40.0%
	CAGGGGGAAGAATTAAATCA+AGG	+	chr5.2:76844930-76844949	None:intergenic	40.0%
	CCAACATTTGTGGTATCTTC+AGG	-	chr5.2:76846301-76846320	MS.gene48021:CDS	40.0%
	CCTGAAGATACCACAAATGT+TGG	+	chr5.2:76846304-76846323	None:intergenic	40.0%
	CGATTGTTGCAAAGTAACTC+AGG	+	chr5.2:76845105-76845124	None:intergenic	40.0%
	CTTGGGTTTAATAGATTGGG+TGG	-	chr5.2:76846213-76846232	MS.gene48021:CDS	40.0%
	GAAAACAAGGTTGCGTTGTT+GGG	+	chr5.2:76845691-76845710	None:intergenic	40.0%
	GAAGTCGTTGCTAATTCCAT+GGG	-	chr5.2:76845075-76845094	MS.gene48021:CDS	40.0%
	GTCATAAAGTGAAGGATGGA+TGG	+	chr5.2:76845747-76845766	None:intergenic	40.0%
	TAAAGTGAAGGATGGATGGA+AGG	+	chr5.2:76845743-76845762	None:intergenic	40.0%
	TATGACCAACGAGGAAATAG+TGG	-	chr5.2:76844861-76844880	MS.gene48021:CDS	40.0%
	TCAAACATCCCACAATGCTA+TGG	+	chr5.2:76845008-76845027	None:intergenic	40.0%
	TCACTAGCCAACTCAAACAA+TGG	-	chr5.2:76845890-76845909	MS.gene48021:intron	40.0%
	TCTAAAGAAATGAGGAGCCT+GGG	+	chr5.2:76846503-76846522	None:intergenic	40.0%
	TGAAGTCGTTGCTAATTCCA+TGG	-	chr5.2:76845074-76845093	MS.gene48021:CDS	40.0%
	TGGGTGCCAGTAGTAATTAT+AGG	+	chr5.2:76844985-76845004	None:intergenic	40.0%
	TTCCCTTCTACAGTTTCATC+TGG	+	chr5.2:76845189-76845208	None:intergenic	40.0%
	TTGTGGTATCTTCAGGACAA+AGG	-	chr5.2:76846308-76846327	MS.gene48021:CDS	40.0%
	TTGTTCTTGTGCAGACAACA+TGG	-	chr5.2:76845540-76845559	MS.gene48021:CDS	40.0%
!	AACCAGTCACCATTCCTTTT+AGG	+	chr5.2:76846436-76846455	None:intergenic	40.0%
!	ACCAGTCACCATTCCTTTTA+GGG	+	chr5.2:76846435-76846454	None:intergenic	40.0%
!	AGTCCTTTTCTGCTTTCTTG+TGG	-	chr5.2:76845939-76845958	MS.gene48021:intron	40.0%
!	ATTTATTGTGGTAGCACTGG+TGG	-	chr5.2:76846268-76846287	MS.gene48021:CDS	40.0%
!	CACTTTTGACCATCGTATAG+CGG	-	chr5.2:76845592-76845611	MS.gene48021:CDS	40.0%
!	GAGTTACTTTGCAACAATCG+TGG	-	chr5.2:76845105-76845124	MS.gene48021:CDS	40.0%
!	GGATTGGCAAGGATTTATTG+TGG	-	chr5.2:76846256-76846275	MS.gene48021:CDS	40.0%
!	TCAGAGTCAAATTCAGGTTC+AGG	+	chr5.2:76845803-76845822	None:intergenic	40.0%
!	TCTTATCATTTTCCTTGGGG+AGG	-	chr5.2:76846379-76846398	MS.gene48021:CDS	40.0%
!!	AGTCGAAGGTTGATTTTGGA+TGG	-	chr5.2:76846338-76846357	MS.gene48021:CDS	40.0%
!!	AGTTCATGAGTTTTTGGAGG+CGG	-	chr5.2:76846171-76846190	MS.gene48021:CDS	40.0%
!!	GAGACTGGTCTAAAGAAATG+AGG	+	chr5.2:76846511-76846530	None:intergenic	40.0%
!!	GATGAAATGTGGTGGATTAG+TGG	-	chr5.2:76845562-76845581	MS.gene48021:CDS	40.0%
!!	GGTTGATAATCCGCTATGTT+GGG	-	chr5.2:76846138-76846157	MS.gene48021:CDS	40.0%
!!	GTCGAAGGTTGATTTTGGAT+GGG	-	chr5.2:76846339-76846358	MS.gene48021:CDS	40.0%
!!	TCGAAGGTTGATTTTGGATG+GGG	-	chr5.2:76846340-76846359	MS.gene48021:CDS	40.0%
!!	TCGAGTTCATGAGTTTTTGG+AGG	-	chr5.2:76846168-76846187	MS.gene48021:CDS	40.0%
!!	TGGTTGATAATCCGCTATGT+TGG	-	chr5.2:76846137-76846156	MS.gene48021:CDS	40.0%
	AAAATGGGGCTTGTGGTTGA+CGG	-	chr5.2:76846010-76846029	MS.gene48021:intron	45.0%
	AACATCCCACAATGCTATGG+TGG	+	chr5.2:76845005-76845024	None:intergenic	45.0%
	AAGAACACACCGACATCAAC+AGG	+	chr5.2:76844949-76844968	None:intergenic	45.0%
	AAGTAACTCAGGTTCACCCA+TGG	+	chr5.2:76845094-76845113	None:intergenic	45.0%
	AATAGGCATCCGCTATACGA+TGG	+	chr5.2:76845604-76845623	None:intergenic	45.0%
	ACAAAGGTTTCCAGAGTCGA+AGG	-	chr5.2:76846324-76846343	MS.gene48021:CDS	45.0%
	ACACCACTAAACCAACCAAG+TGG	+	chr5.2:76845467-76845486	None:intergenic	45.0%
	ACATCCCACAATGCTATGGT+GGG	+	chr5.2:76845004-76845023	None:intergenic	45.0%
	ACTTTGCAACAATCGTGGAG+TGG	-	chr5.2:76845110-76845129	MS.gene48021:CDS	45.0%
	AGAACACACCGACATCAACA+GGG	+	chr5.2:76844948-76844967	None:intergenic	45.0%
	ATCATTGGGCTGAGCCATTT+CGG	+	chr5.2:76845654-76845673	None:intergenic	45.0%
	ATGAAGTGCAAGTGTCCCTT+AGG	+	chr5.2:76846475-76846494	None:intergenic	45.0%
	ATGCCAAGCCCTAAAAGGAA+TGG	-	chr5.2:76846424-76846443	MS.gene48021:CDS	45.0%
	ATTCTTCCCCCTGTTGATGT+CGG	-	chr5.2:76844937-76844956	MS.gene48021:CDS	45.0%
	CAACGTGAATATGACCAACG+AGG	-	chr5.2:76844852-76844871	MS.gene48021:CDS	45.0%
	CCAAAATCAACCTTCGACTC+TGG	+	chr5.2:76846337-76846356	None:intergenic	45.0%
	CGAAAACAAGGTTGCGTTGT+TGG	+	chr5.2:76845692-76845711	None:intergenic	45.0%
	CTAGCCAACTCAAACAATGG+TGG	-	chr5.2:76845893-76845912	MS.gene48021:intron	45.0%
	CTCTACATGCACCTTCCTAA+GGG	-	chr5.2:76846457-76846476	MS.gene48021:CDS	45.0%
	GGAAGAAAGAGGCTAAGCAA+CGG	-	chr5.2:76846031-76846050	MS.gene48021:intron	45.0%
	GTCTAAAGAAATGAGGAGCC+TGG	+	chr5.2:76846504-76846523	None:intergenic	45.0%
	TAAGGGACACTTGCACTTCA+TGG	-	chr5.2:76846474-76846493	MS.gene48021:CDS	45.0%
	TGATAATCCGCTATGTTGGG+TGG	-	chr5.2:76846141-76846160	MS.gene48021:CDS	45.0%
	TGTTGCAAAAATGGGGCTTG+TGG	-	chr5.2:76846003-76846022	MS.gene48021:intron	45.0%
	TTGTGGTTGACGGAAGAAAG+AGG	-	chr5.2:76846020-76846039	MS.gene48021:intron	45.0%
!	GAGTCAAATTCAGGTTCAGG+TGG	+	chr5.2:76845800-76845819	None:intergenic	45.0%
!	TCAAATTCAGGTTCAGGTGG+TGG	+	chr5.2:76845797-76845816	None:intergenic	45.0%
!!	CCAGAGTCGAAGGTTGATTT+TGG	-	chr5.2:76846334-76846353	MS.gene48021:CDS	45.0%
!!	CGAAGGTTGATTTTGGATGG+GGG	-	chr5.2:76846341-76846360	MS.gene48021:CDS	45.0%
!!	GAAATGTGGTGGATTAGTGG+TGG	-	chr5.2:76845565-76845584	MS.gene48021:CDS	45.0%
!!	GAACCACTTGGTTGGTTTAG+TGG	-	chr5.2:76845461-76845480	MS.gene48021:CDS	45.0%
!!	GTGACAGAGGAGCATTTCTT+GGG	-	chr5.2:76846196-76846215	MS.gene48021:CDS	45.0%
!!!	TCACCATTCCTTTTAGGGCT+TGG	+	chr5.2:76846430-76846449	None:intergenic	45.0%
!!!	TTCCTTTTAGGGCTTGGCAT+TGG	+	chr5.2:76846424-76846443	None:intergenic	45.0%
!!!	TTTTGGGACCACCTTGCATA+TGG	-	chr5.2:76845489-76845508	MS.gene48021:CDS	45.0%
	AACACACCGACATCAACAGG+GGG	+	chr5.2:76844946-76844965	None:intergenic	50.0%
	AGCAACGACTTCACCAGCAA+AGG	+	chr5.2:76845067-76845086	None:intergenic	50.0%
	AGCTGCCACTATTTCCTCGT+TGG	+	chr5.2:76844869-76844888	None:intergenic	50.0%
	AGGAGGTAATCCCAAGAGAC+TGG	+	chr5.2:76846526-76846545	None:intergenic	50.0%
	AGTGAAGGATGGATGGAAGG+TGG	+	chr5.2:76845740-76845759	None:intergenic	50.0%
	AGTGCAAGTGTCCCTTAGGA+AGG	+	chr5.2:76846471-76846490	None:intergenic	50.0%
	ATCCTTGCCAATCCAGGAAC+TGG	+	chr5.2:76846250-76846269	None:intergenic	50.0%
	CCATTTCGGCCCATGATACA+AGG	+	chr5.2:76845640-76845659	None:intergenic	50.0%
	CCTTGTATCATGGGCCGAAA+TGG	-	chr5.2:76845637-76845656	MS.gene48021:CDS	50.0%
	GAACACACCGACATCAACAG+GGG	+	chr5.2:76844947-76844966	None:intergenic	50.0%
	GCCCTAAAAGGAATGGTGAC+TGG	-	chr5.2:76846431-76846450	MS.gene48021:CDS	50.0%
	GCTCTACATGCACCTTCCTA+AGG	-	chr5.2:76846456-76846475	MS.gene48021:CDS	50.0%
	GGAAGCCAATGCTCTTGCAT+TGG	+	chr5.2:76844898-76844917	None:intergenic	50.0%
	GTGAAGGATGGATGGAAGGT+GGG	+	chr5.2:76845739-76845758	None:intergenic	50.0%
	GTGCTTTCGATTCCCTATGG+TGG	-	chr5.2:76846097-76846116	MS.gene48021:intron	50.0%
	TGCCAATGCCAAGCCCTAAA+AGG	-	chr5.2:76846419-76846438	MS.gene48021:CDS	50.0%
	TGGTGGAGGTAGATCAGAGA+CGG	+	chr5.2:76845780-76845799	None:intergenic	50.0%
!	AATTCAGGTTCAGGTGGTGG+AGG	+	chr5.2:76845794-76845813	None:intergenic	50.0%
!	CGCTACCAATGCAAGAGCAT+TGG	-	chr5.2:76844890-76844909	MS.gene48021:CDS	50.0%
!!	ACATGGTGTGCTTGCTGTTC+AGG	-	chr5.2:76845227-76845246	MS.gene48021:CDS	50.0%
!!	GGTGACAGAGGAGCATTTCT+TGG	-	chr5.2:76846195-76846214	MS.gene48021:CDS	50.0%
!!	TCATGAGTTTTTGGAGGCGG+CGG	-	chr5.2:76846174-76846193	MS.gene48021:CDS	50.0%
	CGATCTGCCACCCAACATAG+CGG	+	chr5.2:76846151-76846170	None:intergenic	55.0%
	GACAGGCACTCTGTACCCAT+TGG	-	chr5.2:76845372-76845391	MS.gene48021:CDS	55.0%
	GCATCGTCCAGTTCCTGGAT+TGG	-	chr5.2:76846240-76846259	MS.gene48021:CDS	55.0%
	GGCACCCACCATAGCATTGT+GGG	-	chr5.2:76844997-76845016	MS.gene48021:CDS	55.0%
	GGGACACTTGCACTTCATGG+AGG	-	chr5.2:76846477-76846496	MS.gene48021:CDS	55.0%
	GGTGGAGGTAGATCAGAGAC+GGG	+	chr5.2:76845779-76845798	None:intergenic	55.0%
	GTGGAGGTAGATCAGAGACG+GGG	+	chr5.2:76845778-76845797	None:intergenic	55.0%
	TGGCACCCACCATAGCATTG+TGG	-	chr5.2:76844996-76845015	MS.gene48021:CDS	55.0%
	TTTCCTTGGGGAGGTAGTGC+TGG	-	chr5.2:76846388-76846407	MS.gene48021:CDS	55.0%
!!	GAAGAGCATCGTCCAGTTCC+TGG	-	chr5.2:76846235-76846254	MS.gene48021:CDS	55.0%
!!	GGTAGCACTGGTGGAGAAGA+AGG	-	chr5.2:76846277-76846296	MS.gene48021:CDS	55.0%
!!	GTCCAGTTCCTGGATTGGCA+AGG	-	chr5.2:76846245-76846264	MS.gene48021:CDS	55.0%
!!!	GGACTCGAGTTTTGAGCGCT+TGG	+	chr5.2:76845924-76845943	None:intergenic	55.0%
	CTTGCACTTCATGGAGGCCC+AGG	-	chr5.2:76846483-76846502	MS.gene48021:CDS	60.0%
	GGATGGGGGAAGCCTGTGTT+TGG	-	chr5.2:76846355-76846374	MS.gene48021:CDS	60.0%
	TAGCCAGCACTACCTCCCCA+AGG	+	chr5.2:76846394-76846413	None:intergenic	60.0%
	TTTGGAGGCGGCGGTGACAG+AGG	-	chr5.2:76846183-76846202	MS.gene48021:CDS	65.0%
!	GATGGAAGGTGGGCGTCGTG+GGG	+	chr5.2:76845729-76845748	None:intergenic	65.0%
!	GGATGGAAGGTGGGCGTCGT+GGG	+	chr5.2:76845730-76845749	None:intergenic	65.0%
!	TGGATGGAAGGTGGGCGTCG+TGG	+	chr5.2:76845731-76845750	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	76844829	76846552	76844829	ID=MS.gene48021
chr5.2	mRNA	76844829	76846552	76844829	ID=MS.gene48021.t1;Parent=MS.gene48021
chr5.2	exon	76846133	76846552	76846133	ID=MS.gene48021.t1.exon1;Parent=MS.gene48021.t1
chr5.2	CDS	76846133	76846552	76846133	ID=cds.MS.gene48021.t1;Parent=MS.gene48021.t1
chr5.2	exon	76844829	76845827	76844829	ID=MS.gene48021.t1.exon2;Parent=MS.gene48021.t1
chr5.2	CDS	76844829	76845827	76844829	ID=cds.MS.gene48021.t1;Parent=MS.gene48021.t1

Gene Sequence

>MS.gene48021

ATGTGTATTGGAAAAGTAGAACTCAACGTGAATATGACCAACGAGGAAATAGTGGCAGCTGCGCTACCAATGCAAGAGCATTGGCTTCCACTATCAAACCTTGATTTAATTCTTCCCCCTGTTGATGTCGGTGTGTTCTTTTGCTACAATAAGCCTATAATTACTACTGGCACCCACCATAGCATTGTGGGATGTTTGAAAAATTCTTTAGCTAAGGCTCTTGTTTCTTATTATGCCTTTGCTGGTGAAGTCGTTGCTAATTCCATGGGTGAACCTGAGTTACTTTGCAACAATCGTGGAGTGGATTTTGTTGAAGCTTTTGCTGATGTTGAGTTACAATCTCTAAATTTGTATAATCCAGATGAAACTGTAGAAGGGAAGCTTGTTCCAAAGAAGAAACATGGTGTGCTTGCTGTTCAGGTGAAAAAAGATTTATTTTATTACCAAAACATTTTGTTCTCAAGGGAATATTCGAATAGGCAAAGAATTATTATAAGCATGTACTATTATAATAATCATTATAATTATTAGTACTTGTGTTTAGACAGGCACTCTGTACCCATTGGTCTTATCATTTTACTGCACTGATGCGAATAAATTACGAACAATAATTTTTATTAAATTTTACTTTTGAACCACTTGGTTGGTTTAGTGGTGTTGTTTTGGGACCACCTTGCATATGGTATAGATTATAAAGTGTTTTAATCGTCATTGTTCTTGTGCAGACAACATGGATGAAATGTGGTGGATTAGTGGTGGCTTGCACTTTTGACCATCGTATAGCGGATGCCTATTCTGCAAACATGTTCCTTGTATCATGGGCCGAAATGGCTCAGCCCAATGATAACAAGTCTTTGATCCCAACAACGCAACCTTGTTTTCGACAGTCACTTCTAACCCCACGACGCCCACCTTCCATCCATCCTTCACTTTATGACATGTATGTCCCCGTCTCTGATCTACCTCCACCACCTGAACCTGAATTTGACTCTGATATTAAAACTAACCCAATAATAAGTTGCATATACTATGTAACAAGCAAAGAACTCAACAACATGCAATCACTAGCCAACTCAAACAATGGTGGTAGTTCCAAGCGCTCAAAACTCGAGTCCTTTTCTGCTTTCTTGTGGAAAATGGTTGCGGAAGCCGCTTCAATCGATAATGAAAACATTGTTGCAAAAATGGGGCTTGTGGTTGACGGAAGAAAGAGGCTAAGCAACGGTGACAAAAACAAAGAAGCGATTATGAATTCATATTTTGGGAATGTGCTTTCGATTCCCTATGGTGGTAGATTAACAAAAGAGCTGGTTGATAATCCGCTATGTTGGGTGGCAGATCGAGTTCATGAGTTTTTGGAGGCGGCGGTGACAGAGGAGCATTTCTTGGGTTTAATAGATTGGGTGGAAGAGCATCGTCCAGTTCCTGGATTGGCAAGGATTTATTGTGGTAGCACTGGTGGAGAAGAAGGACCAACATTTGTGGTATCTTCAGGACAAAGGTTTCCAGAGTCGAAGGTTGATTTTGGATGGGGGAAGCCTGTGTTTGGATCTTATCATTTTCCTTGGGGAGGTAGTGCTGGCTATGTTATGCCAATGCCAAGCCCTAAAAGGAATGGTGACTGGTTGCTCTACATGCACCTTCCTAAGGGACACTTGCACTTCATGGAGGCCCAGGCTCCTCATTTCTTTAGACCAGTCTCTTGGGATTACCTCCTCAATTAA

Protein sequence

>MS.gene48021.t1

MCIGKVELNVNMTNEEIVAAALPMQEHWLPLSNLDLILPPVDVGVFFCYNKPIITTGTHHSIVGCLKNSLAKALVSYYAFAGEVVANSMGEPELLCNNRGVDFVEAFADVELQSLNLYNPDETVEGKLVPKKKHGVLAVQTTWMKCGGLVVACTFDHRIADAYSANMFLVSWAEMAQPNDNKSLIPTTQPCFRQSLLTPRRPPSIHPSLYDMYVPVSDLPPPPEPEFDSDIKTNPIISCIYYVTSKELNNMQSLANSNNGGSSKRSKLESFSAFLWKMVAEAASIDNENIVAKMGLVVDGRKRLSNGDKNKEAIMNSYFGNVLSIPYGGRLTKELVDNPLCWVADRVHEFLEAAVTEEHFLGLIDWVEEHRPVPGLARIYCGSTGGEEGPTFVVSSGQRFPESKVDFGWGKPVFGSYHFPWGGSAGYVMPMPSPKRNGDWLLYMHLPKGHLHFMEAQAPHFFRPVSWDYLLN