AlfalfaGEDB | MS.gene017053

M. sativa cultivar XinJiangDaYe / MS.gene017053

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017053.t1	XP_003617368.1	93.4	473	28	2	1	472	1	471	1.00E-264	922.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017053.t1	Q9FI78	24.8	459	302	16	8	455	1	427	9.5e-30	132.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017053.t1	G7K3A2	93.4	473	28	2	1	472	1	471	7.2e-265	922.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene017053	MS.gene039597	PPI
MS.gene017053	MS.gene38365	PPI
MS.gene38704	MS.gene017053	PPI
MS.gene017053	MS.gene012961	PPI
MS.gene017053	MS.gene038274	PPI
MS.gene38361	MS.gene017053	PPI
MS.gene017053	MS.gene38363	PPI
MS.gene046246	MS.gene017053	PPI
MS.gene017053	MS.gene38701	PPI
MS.gene017053	MS.gene69496	PPI
MS.gene017053	MS.gene38359	PPI
MS.gene38700	MS.gene017053	PPI
MS.gene012960	MS.gene017053	PPI
MS.gene012965	MS.gene017053	PPI
MS.gene017053	MS.gene019178	PPI
MS.gene017053	MS.gene38699	PPI
MS.gene017053	MS.gene32933	PPI
MS.gene017053	MS.gene028134	PPI
MS.gene017053	MS.gene47721	PPI
MS.gene017053	MS.gene38706	PPI
MS.gene017053	MS.gene012959	PPI
MS.gene017053	MS.gene017054	PPI
MS.gene017053	MS.gene012957	PPI
MS.gene017053	MS.gene82145	PPI
MS.gene95242	MS.gene017053	PPI
MS.gene32936	MS.gene017053	PPI
MS.gene72785	MS.gene017053	PPI
MS.gene017053	MS.gene038271	PPI
MS.gene017053	MS.gene32934	PPI
MS.gene48027	MS.gene017053	PPI
MS.gene017058	MS.gene017053	PPI
MS.gene32938	MS.gene017053	PPI
MS.gene38362	MS.gene017053	PPI
MS.gene017053	MS.gene22199	PPI
MS.gene017053	MS.gene47719	PPI
MS.gene47716	MS.gene017053	PPI
MS.gene017053	MS.gene069321	PPI
MS.gene017053	MS.gene000507	PPI
MS.gene017053	MS.gene38702	PPI
MS.gene83461	MS.gene017053	PPI
MS.gene48021	MS.gene017053	PPI
MS.gene48023	MS.gene017053	PPI
MS.gene017053	MS.gene38703	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017053.t1	MTR_5g090830	93.446	473	28	2	1	472	1	471	0.0	920
MS.gene017053.t1	MTR_5g090810	92.178	473	34	2	1	472	1	471	0.0	909
MS.gene017053.t1	MTR_5g090880	80.127	473	93	1	1	472	1	473	0.0	779
MS.gene017053.t1	MTR_3g450710	68.085	470	129	7	10	470	11	468	0.0	630
MS.gene017053.t1	MTR_3g450690	65.106	470	147	5	10	470	11	472	0.0	614
MS.gene017053.t1	MTR_5g026320	36.804	413	233	11	31	440	41	428	7.76e-72	234
MS.gene017053.t1	MTR_8g096530	27.489	462	286	18	7	458	4	426	4.42e-33	130
MS.gene017053.t1	MTR_8g075610	25.057	435	279	14	32	455	16	414	6.42e-32	127
MS.gene017053.t1	MTR_2g064495	24.838	463	292	17	10	455	3	426	1.73e-30	123
MS.gene017053.t1	MTR_2g105330	24.838	463	292	17	10	455	3	426	7.20e-30	121
MS.gene017053.t1	MTR_4g007540	23.671	414	279	12	10	414	3	388	3.29e-28	116
MS.gene017053.t1	MTR_4g097090	25.216	464	300	15	23	465	19	456	5.06e-28	116
MS.gene017053.t1	MTR_4g127650	23.861	461	309	12	23	465	20	456	2.11e-27	114
MS.gene017053.t1	MTR_3g049150	25.285	439	275	17	31	446	23	431	2.80e-27	114
MS.gene017053.t1	MTR_3g098980	26.735	389	241	14	31	411	35	387	3.32e-27	113
MS.gene017053.t1	MTR_7g086000	29.111	371	215	15	66	411	60	407	5.69e-27	113
MS.gene017053.t1	MTR_5g076700	25.253	396	249	13	66	447	65	427	2.95e-26	111
MS.gene017053.t1	MTR_7g114470	25.592	422	264	16	8	415	9	394	8.19e-26	110
MS.gene017053.t1	MTR_7g114520	25.926	459	281	18	8	446	9	428	1.95e-25	108
MS.gene017053.t1	MTR_7g114560	23.929	397	258	11	63	447	62	426	3.17e-25	108
MS.gene017053.t1	MTR_1g040215	26.368	402	233	14	63	436	61	427	4.97e-25	107
MS.gene017053.t1	MTR_7g027175	25.416	421	268	15	4	414	9	393	1.13e-24	106
MS.gene017053.t1	MTR_1g081660	26.273	373	229	15	63	424	70	407	2.53e-24	105
MS.gene017053.t1	MTR_1g040175	25.310	403	236	15	63	436	61	427	4.05e-24	105
MS.gene017053.t1	MTR_7g114550	24.301	465	295	16	1	447	1	426	4.24e-24	105
MS.gene017053.t1	MTR_7g114490	24.029	412	261	13	63	458	62	437	9.32e-23	101
MS.gene017053.t1	MTR_1g053315	24.570	407	253	15	29	415	40	412	1.26e-21	97.8
MS.gene017053.t1	MTR_3g026950	24.083	436	288	13	31	446	23	435	7.23e-21	95.1
MS.gene017053.t1	MTR_7g085930	27.595	395	234	19	66	429	59	432	1.42e-20	94.7
MS.gene017053.t1	MTR_4g055170	24.176	455	297	18	17	451	19	445	3.10e-18	87.4
MS.gene017053.t1	MTR_1g040195	24.523	367	216	14	96	436	71	402	6.51e-18	86.3
MS.gene017053.t1	MTR_2g015430	23.810	399	257	17	24	410	18	381	1.58e-17	85.1
MS.gene017053.t1	MTR_1g040320	23.573	403	207	16	63	436	61	391	1.29e-16	82.0
MS.gene017053.t1	MTR_7g084940	25.641	429	247	18	6	411	12	391	1.30e-16	82.4
MS.gene017053.t1	MTR_3g086020	23.678	435	255	20	6	426	1	372	5.16e-16	80.1
MS.gene017053.t1	MTR_3g101960	23.602	322	197	12	49	348	48	342	5.25e-16	80.5
MS.gene017053.t1	MTR_5g084870	22.803	421	267	16	8	414	1	377	1.23e-15	79.3
MS.gene017053.t1	MTR_7g084910	25.217	460	257	19	7	434	14	418	7.26e-15	77.0
MS.gene017053.t1	MTR_3g070170	23.377	385	235	13	60	411	60	417	8.89e-15	76.6
MS.gene017053.t1	MTR_3g112180	22.143	420	250	15	18	411	20	388	1.57e-14	75.9
MS.gene017053.t1	MTR_7g114510	24.727	275	175	9	142	415	9	252	3.86e-14	73.6
MS.gene017053.t1	MTR_4g094842	24.468	376	217	13	66	413	62	398	8.60e-14	73.6
MS.gene017053.t1	MTR_7g110610	28.947	152	93	3	66	202	55	206	1.20e-12	69.7
MS.gene017053.t1	MTR_3g088420	24.060	399	237	16	36	413	32	385	2.37e-12	68.9
MS.gene017053.t1	MTR_5g084850	22.459	423	265	14	8	414	1	376	2.50e-12	68.9
MS.gene017053.t1	MTR_8g041770	23.971	413	249	15	23	413	22	391	7.94e-12	67.4
MS.gene017053.t1	MTR_4g094828	22.943	401	244	15	36	411	37	397	9.65e-12	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017053.t1	AT1G32910	58.263	472	164	11	8	470	11	458	1.78e-178	508
MS.gene017053.t1	AT1G78990	56.745	467	174	9	8	470	11	453	5.44e-178	506
MS.gene017053.t1	AT5G16410	51.368	475	190	14	8	470	34	479	1.23e-157	456
MS.gene017053.t1	AT1G31490	36.652	442	238	12	8	440	4	412	7.51e-77	247
MS.gene017053.t1	AT5G48930	24.946	461	299	17	8	455	1	427	1.13e-34	134
MS.gene017053.t1	AT2G19070	24.034	466	310	16	18	465	12	451	5.45e-27	113
MS.gene017053.t1	AT5G41040	25.127	394	250	13	31	414	38	396	2.49e-26	111
MS.gene017053.t1	AT5G41040	25.127	394	250	13	31	414	54	412	3.53e-26	111
MS.gene017053.t1	AT5G57840	26.282	468	280	19	8	451	1	427	4.59e-26	110
MS.gene017053.t1	AT3G03480	25.647	464	270	19	6	436	15	436	2.34e-24	105
MS.gene017053.t1	AT3G48720	26.835	395	240	13	31	414	30	386	6.67e-24	103
MS.gene017053.t1	AT5G17540	24.786	468	265	19	4	439	3	415	6.11e-23	101
MS.gene017053.t1	AT3G50270	26.570	414	236	18	60	451	56	423	1.01e-19	92.0
MS.gene017053.t1	AT1G27620	21.945	401	266	13	66	455	58	422	1.60e-19	91.3
MS.gene017053.t1	AT5G63560	25.245	408	246	14	19	413	20	381	3.14e-19	90.1
MS.gene017053.t1	AT1G24430	25.355	422	247	18	8	411	3	374	1.64e-18	88.2
MS.gene017053.t1	AT1G65450	26.076	395	243	15	31	411	40	399	3.55e-18	87.0
MS.gene017053.t1	AT1G28680	25.240	416	262	19	12	410	1	384	8.05e-17	83.2
MS.gene017053.t1	AT3G30280	25.285	439	266	14	8	431	4	395	3.73e-16	80.9
MS.gene017053.t1	AT5G67150	23.953	430	256	16	62	468	57	438	4.21e-16	80.9
MS.gene017053.t1	AT5G07080	24.185	368	237	10	60	413	64	403	1.12e-15	79.3
MS.gene017053.t1	AT5G38130	23.785	391	240	16	60	429	73	426	1.03e-14	76.6
MS.gene017053.t1	AT5G47950	25.263	285	171	9	8	284	4	254	4.86e-14	74.3
MS.gene017053.t1	AT4G15390	24.490	343	210	13	8	334	6	315	5.69e-14	74.3
MS.gene017053.t1	AT4G15390	24.490	343	210	13	8	334	6	315	5.69e-14	74.3
MS.gene017053.t1	AT5G01210	25.131	382	201	15	77	411	73	416	5.88e-14	74.3
MS.gene017053.t1	AT5G42830	23.352	364	234	13	66	413	64	398	8.11e-14	73.9
MS.gene017053.t1	AT1G24420	22.902	441	261	17	6	419	1	389	9.56e-13	70.5
MS.gene017053.t1	AT1G24420	22.902	441	261	17	6	419	1	389	9.56e-13	70.5
MS.gene017053.t1	AT5G67160	24.424	434	254	20	59	472	49	428	9.58e-13	70.5
MS.gene017053.t1	AT2G25150	22.520	373	235	13	63	421	64	396	9.63e-13	70.5
MS.gene017053.t1	AT3G50290	24.286	280	177	11	136	410	20	269	1.35e-11	65.5

Find 128 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGATTATGAATTCATATTT+TGG	0.139298	5.1:-72172629	MS.gene017053:CDS
TTCTGCTTTCTTGTGGAAAA+TGG	0.174817	5.1:-72172753	MS.gene017053:CDS
CCAGAGTCGAAGGTTGATTT+TGG	0.189640	5.1:-72172365	MS.gene017053:CDS
TAGTATATGCGACTTATTAT+TGG	0.243557	5.1:+72172863	None:intergenic
AGCACACCATGTTTCTTCTT+TGG	0.268793	5.1:+72173483	None:intergenic
GTTTCTTATTATGCCTTTGC+TGG	0.282844	5.1:-72173648	MS.gene017053:CDS
TTAATATCAGAGTCAAATTC+AGG	0.299615	5.1:+72172893	None:intergenic
ACATGGTGTGCTTGCTGTTC+AGG	0.312822	5.1:-72173472	MS.gene017053:intron
GGATGGAAGGTGGGCGTCGT+GGG	0.321410	5.1:+72172972	None:intergenic
AGTATATGCGACTTATTATT+GGG	0.330800	5.1:+72172864	None:intergenic
AAGAACACACCGACATCAAC+AGG	0.337593	5.1:+72173753	None:intergenic
ATCATTGGGCTGAGCCATTT+CGG	0.339987	5.1:+72173048	None:intergenic
TAAGGGACACTTGCACTTCA+TGG	0.343370	5.1:-72172225	MS.gene017053:CDS
GGTGACAGAGGAGCATTTCT+TGG	0.346724	5.1:-72172504	MS.gene017053:CDS
ATTTCTTGGGTTTAATAGAT+TGG	0.349608	5.1:-72172490	MS.gene017053:CDS
GAAGAGCATCGTCCAGTTCC+TGG	0.354253	5.1:-72172464	MS.gene017053:CDS
TGGATGGAAGGTGGGCGTCG+TGG	0.357861	5.1:+72172971	None:intergenic
TGCCAATGCCAAGCCCTAAA+AGG	0.358606	5.1:-72172280	MS.gene017053:CDS
TTTCCTTGGGGAGGTAGTGC+TGG	0.358995	5.1:-72172311	MS.gene017053:CDS
AAGGAACATGTTTGCAGAAT+AGG	0.360809	5.1:+72173081	None:intergenic
TTCCCTTCTACAGTTTCATC+TGG	0.371632	5.1:+72173513	None:intergenic
CGAAAACAAGGTTGCGTTGT+TGG	0.373331	5.1:+72173010	None:intergenic
ATTTCTTTAGACCAGTCTCT+TGG	0.380544	5.1:-72172187	MS.gene017053:CDS
GATCAAAGACTTGTTATCAT+TGG	0.392905	5.1:+72173033	None:intergenic
TTTCTTGGGTTTAATAGATT+GGG	0.393187	5.1:-72172489	MS.gene017053:CDS
CCAAAATCAACCTTCGACTC+TGG	0.403209	5.1:+72172365	None:intergenic
ATCAAAGACTTGTTATCATT+GGG	0.404681	5.1:+72173034	None:intergenic
TCAGAGTCAAATTCAGGTTC+AGG	0.411350	5.1:+72172899	None:intergenic
CAAACATGTTCCTTGTATCA+TGG	0.413069	5.1:-72173072	MS.gene017053:CDS
CTAATCGCTAAAATGTGTAT+TGG	0.419429	5.1:-72173882	None:intergenic
TTTCTTTAGACCAGTCTCTT+GGG	0.423523	5.1:-72172186	MS.gene017053:CDS
ATCCTTGCCAATCCAGGAAC+TGG	0.426912	5.1:+72172452	None:intergenic
CCTTGTATCATGGGCCGAAA+TGG	0.428090	5.1:-72173062	MS.gene017053:CDS
CCAACATTTGTGGTATCTTC+AGG	0.429306	5.1:-72172398	MS.gene017053:CDS
ATTAAATCAAGGTTTGATAG+TGG	0.431310	5.1:+72173783	None:intergenic
GAAAACAAGGTTGCGTTGTT+GGG	0.433945	5.1:+72173011	None:intergenic
AATCCAGATGAAACTGTAGA+AGG	0.442984	5.1:-72173516	MS.gene017053:CDS
GTGACAGAGGAGCATTTCTT+GGG	0.453426	5.1:-72172503	MS.gene017053:CDS
ATGAAGTGCAAGTGTCCCTT+AGG	0.455932	5.1:+72172227	None:intergenic
TTTCCACAAGAAAGCAGAAA+AGG	0.464789	5.1:+72172757	None:intergenic
CGCTACCAATGCAAGAGCAT+TGG	0.468968	5.1:-72173809	MS.gene017053:CDS
GCTCTACATGCACCTTCCTA+AGG	0.469205	5.1:-72172243	MS.gene017053:CDS
GTCTAAAGAAATGAGGAGCC+TGG	0.472034	5.1:+72172198	None:intergenic
TGGGTGCCAGTAGTAATTAT+AGG	0.473421	5.1:+72173717	None:intergenic
TATGACCAACGAGGAAATAG+TGG	0.494458	5.1:-72173838	MS.gene017053:CDS
AGCTGCCACTATTTCCTCGT+TGG	0.494647	5.1:+72173833	None:intergenic
CAATAAATCCTTGCCAATCC+AGG	0.495236	5.1:+72172446	None:intergenic
GATGAAATGTGGTGGATTAG+TGG	0.498028	5.1:-72173137	MS.gene017053:CDS
GGCACCCACCATAGCATTGT+GGG	0.501789	5.1:-72173702	MS.gene017053:CDS
AGGATTTATTGTGGTAGCAC+TGG	0.506494	5.1:-72172434	MS.gene017053:CDS
GGTTGATAATCCGCTATGTT+GGG	0.513429	5.1:-72172561	MS.gene017053:CDS
TCAAATTCAGGTTCAGGTGG+TGG	0.515057	5.1:+72172905	None:intergenic
CTTGCACTTCATGGAGGCCC+AGG	0.516007	5.1:-72172216	MS.gene017053:CDS
CCATTTCGGCCCATGATACA+AGG	0.516374	5.1:+72173062	None:intergenic
ACTACCACCATTGTTTGAGT+TGG	0.517260	5.1:+72172802	None:intergenic
GTGAAGGATGGATGGAAGGT+GGG	0.517707	5.1:+72172963	None:intergenic
GCATCGTCCAGTTCCTGGAT+TGG	0.519656	5.1:-72172459	MS.gene017053:CDS
TCAAACATCCCACAATGCTA+TGG	0.522391	5.1:+72173694	None:intergenic
GTCATAAAGTGAAGGATGGA+TGG	0.526884	5.1:+72172955	None:intergenic
TTTGTTAATCTACCACCATA+GGG	0.527591	5.1:+72172590	None:intergenic
TCACTAGCCAACTCAAACAA+TGG	0.529140	5.1:-72172809	MS.gene017053:CDS
GGTAGCACTGGTGGAGAAGA+AGG	0.530503	5.1:-72172422	MS.gene017053:CDS
TTTCTTGTGGAAAATGGTTG+CGG	0.534078	5.1:-72172747	MS.gene017053:CDS
ATCCAGATGAAACTGTAGAA+GGG	0.535674	5.1:-72173515	MS.gene017053:CDS
AGTGAAGGATGGATGGAAGG+TGG	0.539276	5.1:+72172962	None:intergenic
ATTTATTGTGGTAGCACTGG+TGG	0.539802	5.1:-72172431	MS.gene017053:CDS
AAACATGTTCCTTGTATCAT+GGG	0.539896	5.1:-72173071	MS.gene017053:CDS
TGGTTGATAATCCGCTATGT+TGG	0.541576	5.1:-72172562	MS.gene017053:CDS
AGAAGAAGGACCAACATTTG+TGG	0.543509	5.1:-72172408	MS.gene017053:CDS
TGAAGTCGTTGCTAATTCCA+TGG	0.543846	5.1:-72173625	MS.gene017053:CDS
ACAAACACTAATATTAATTG+AGG	0.550824	5.1:+72172156	None:intergenic
AATTCAGGTTCAGGTGGTGG+AGG	0.553611	5.1:+72172908	None:intergenic
AATAAGCCTATAATTACTAC+TGG	0.553926	5.1:-72173723	MS.gene017053:CDS
TTTGGAGGCGGCGGTGACAG+AGG	0.555868	5.1:-72172516	MS.gene017053:CDS
GCCCTAAAAGGAATGGTGAC+TGG	0.557544	5.1:-72172268	MS.gene017053:CDS
AGGAGGTAATCCCAAGAGAC+TGG	0.562245	5.1:+72172176	None:intergenic
TGGTAGATTAACAAAAGAGC+TGG	0.565630	5.1:-72172582	MS.gene017053:CDS
AACATCCCACAATGCTATGG+TGG	0.566613	5.1:+72173697	None:intergenic
CTTGGGTTTAATAGATTGGG+TGG	0.568431	5.1:-72172486	MS.gene017053:CDS
CGATTGTTGCAAAGTAACTC+AGG	0.570367	5.1:+72173597	None:intergenic
TCTAAAGAAATGAGGAGCCT+GGG	0.578551	5.1:+72172199	None:intergenic
CCTGAAGATACCACAAATGT+TGG	0.579526	5.1:+72172398	None:intergenic
GATGGAAGGTGGGCGTCGTG+GGG	0.580404	5.1:+72172973	None:intergenic
AGAAGTGACTGTCGAAAACA+AGG	0.582200	5.1:+72172998	None:intergenic
GGAAGCCAATGCTCTTGCAT+TGG	0.585364	5.1:+72173804	None:intergenic
ACAAAGGTTTCCAGAGTCGA+AGG	0.585762	5.1:-72172375	MS.gene017053:CDS
GGTGGAGGTAGATCAGAGAC+GGG	0.587879	5.1:+72172923	None:intergenic
AGCAACGACTTCACCAGCAA+AGG	0.588840	5.1:+72173635	None:intergenic
TAAAGTGAAGGATGGATGGA+AGG	0.590822	5.1:+72172959	None:intergenic
TTGTGGTATCTTCAGGACAA+AGG	0.593564	5.1:-72172391	MS.gene017053:CDS
ACATGTCATAAAGTGAAGGA+TGG	0.594714	5.1:+72172951	None:intergenic
ATGCCAAGCCCTAAAAGGAA+TGG	0.595010	5.1:-72172275	MS.gene017053:CDS
TGGTGGAGGTAGATCAGAGA+CGG	0.600538	5.1:+72172922	None:intergenic
CTCTACATGCACCTTCCTAA+GGG	0.601014	5.1:-72172242	MS.gene017053:CDS
AGTGCAAGTGTCCCTTAGGA+AGG	0.602305	5.1:+72172231	None:intergenic
AACACTAATATTAATTGAGG+AGG	0.603374	5.1:+72172159	None:intergenic
TTGTGGTTGACGGAAGAAAG+AGG	0.605169	5.1:-72172679	MS.gene017053:CDS
GAGTTACTTTGCAACAATCG+TGG	0.605999	5.1:-72173594	MS.gene017053:CDS
GTCCAGTTCCTGGATTGGCA+AGG	0.607030	5.1:-72172454	MS.gene017053:CDS
TTGTTCTTGTGCAGACAACA+TGG	0.607336	5.1:-72173159	MS.gene017053:intron
AATGTGCTTTCGATTCCCTA+TGG	0.610089	5.1:-72172605	MS.gene017053:CDS
GAGACTGGTCTAAAGAAATG+AGG	0.612013	5.1:+72172191	None:intergenic
AGAACACACCGACATCAACA+GGG	0.612828	5.1:+72173754	None:intergenic
AAGTAACTCAGGTTCACCCA+TGG	0.618644	5.1:+72173608	None:intergenic
GTGCTTTCGATTCCCTATGG+TGG	0.619185	5.1:-72172602	MS.gene017053:CDS
AAAATGGGGCTTGTGGTTGA+CGG	0.619932	5.1:-72172689	MS.gene017053:CDS
CTTGTTCCAAAGAAGAAACA+TGG	0.620389	5.1:-72173489	MS.gene017053:CDS
TGGCACCCACCATAGCATTG+TGG	0.626128	5.1:-72173703	MS.gene017053:CDS
ACATCCCACAATGCTATGGT+GGG	0.631579	5.1:+72173698	None:intergenic
ACATACATGTCATAAAGTGA+AGG	0.639724	5.1:+72172947	None:intergenic
ACTTTGCAACAATCGTGGAG+TGG	0.644603	5.1:-72173589	MS.gene017053:CDS
GAAGTCGTTGCTAATTCCAT+GGG	0.644829	5.1:-72173624	MS.gene017053:CDS
GAACACACCGACATCAACAG+GGG	0.646980	5.1:+72173755	None:intergenic
GGATTGGCAAGGATTTATTG+TGG	0.648587	5.1:-72172443	MS.gene017053:CDS
GAGTCAAATTCAGGTTCAGG+TGG	0.648966	5.1:+72172902	None:intergenic
TGATAATCCGCTATGTTGGG+TGG	0.663374	5.1:-72172558	MS.gene017053:CDS
ACAACATGGATGAAATGTGG+TGG	0.668180	5.1:-72173145	MS.gene017053:CDS
AATAGGCATCCGCTATACGA+TGG	0.674136	5.1:+72173098	None:intergenic
GAAATGTGGTGGATTAGTGG+TGG	0.677628	5.1:-72173134	MS.gene017053:CDS
AACACACCGACATCAACAGG+GGG	0.687180	5.1:+72173756	None:intergenic
CAGACAACATGGATGAAATG+TGG	0.692012	5.1:-72173148	MS.gene017053:CDS
CTAGCCAACTCAAACAATGG+TGG	0.694488	5.1:-72172806	MS.gene017053:CDS
TAGCCAGCACTACCTCCCCA+AGG	0.698597	5.1:+72172308	None:intergenic
GGGACACTTGCACTTCATGG+AGG	0.714604	5.1:-72172222	MS.gene017053:CDS
CGATCTGCCACCCAACATAG+CGG	0.715687	5.1:+72172551	None:intergenic
GGAAGAAAGAGGCTAAGCAA+CGG	0.723389	5.1:-72172668	MS.gene017053:CDS
CAACGTGAATATGACCAACG+AGG	0.763069	5.1:-72173847	MS.gene017053:CDS
GTGGAGGTAGATCAGAGACG+GGG	0.788848	5.1:+72172924	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTGAAAAATTCTTTAGCTA+AGG	-	chr5.1:72172363-72172382	MS.gene017053:CDS	20.0%
!!!	AATTTTAATTTTGAACCACT+TGG	-	chr5.1:72172789-72172808	MS.gene017053:CDS	20.0%
!!!	CGATTATGAATTCATATTTT+GGG	-	chr5.1:72173411-72173430	MS.gene017053:intron	20.0%
!	AAAACATTGTTGCAAAAATG+GGG	-	chr5.1:72173336-72173355	MS.gene017053:intron	25.0%
!	AATAAGCCTATAATTACTAC+TGG	-	chr5.1:72172316-72172335	MS.gene017053:CDS	25.0%
!	AGTAAAATGATAAGACCAAT+GGG	+	chr5.1:72172730-72172749	None:intergenic	25.0%
!	AGTATATGCGACTTATTATT+GGG	+	chr5.1:72173178-72173197	None:intergenic	25.0%
!	ATTAAATCAAGGTTTGATAG+TGG	+	chr5.1:72172259-72172278	None:intergenic	25.0%
!	ATTTCTTGGGTTTAATAGAT+TGG	-	chr5.1:72173549-72173568	MS.gene017053:CDS	25.0%
!	GAAAACATTGTTGCAAAAAT+GGG	-	chr5.1:72173335-72173354	MS.gene017053:intron	25.0%
!	TAGTATATGCGACTTATTAT+TGG	+	chr5.1:72173179-72173198	None:intergenic	25.0%
!	TATAATCTATACCATATGCA+TGG	+	chr5.1:72172843-72172862	None:intergenic	25.0%
!	TGAAAACATTGTTGCAAAAA+TGG	-	chr5.1:72173334-72173353	MS.gene017053:intron	25.0%
!	TTAATATCAGAGTCAAATTC+AGG	+	chr5.1:72173149-72173168	None:intergenic	25.0%
!	TTTCTTGGGTTTAATAGATT+GGG	-	chr5.1:72173550-72173569	MS.gene017053:CDS	25.0%
!!	GCGATTATGAATTCATATTT+TGG	-	chr5.1:72173410-72173429	MS.gene017053:intron	25.0%
!!!	ATCAAAGACTTGTTATCATT+GGG	+	chr5.1:72173008-72173027	None:intergenic	25.0%
	AAACATGTTCCTTGTATCAT+GGG	-	chr5.1:72172968-72172987	MS.gene017053:CDS	30.0%
	ACATACATGTCATAAAGTGA+AGG	+	chr5.1:72173095-72173114	None:intergenic	30.0%
	ATTAGTACTTGTGTTTAGAC+AGG	-	chr5.1:72172695-72172714	MS.gene017053:CDS	30.0%
	CAGTAAAATGATAAGACCAA+TGG	+	chr5.1:72172731-72172750	None:intergenic	30.0%
	TTTGTTAATCTACCACCATA+GGG	+	chr5.1:72173452-72173471	None:intergenic	30.0%
!	ACCAAAACATTTTGTTCTCA+AGG	-	chr5.1:72172611-72172630	MS.gene017053:CDS	30.0%
!	CCAAAACATTTTGTTCTCAA+GGG	-	chr5.1:72172612-72172631	MS.gene017053:CDS	30.0%
!	TGGATCTTATCATTTTCCTT+GGG	-	chr5.1:72173715-72173734	MS.gene017053:CDS	30.0%
!	TTAATTTTGAACCACTTGGT+TGG	-	chr5.1:72172793-72172812	MS.gene017053:CDS	30.0%
!	TTGGATCTTATCATTTTCCT+TGG	-	chr5.1:72173714-72173733	MS.gene017053:CDS	30.0%
!	TTTTGTTAATCTACCACCAT+AGG	+	chr5.1:72173453-72173472	None:intergenic	30.0%
!!	GATCAAAGACTTGTTATCAT+TGG	+	chr5.1:72173009-72173028	None:intergenic	30.0%
!!	GTTTTCATTATCGATTGAAG+CGG	+	chr5.1:72173321-72173340	None:intergenic	30.0%
	AAGGAACATGTTTGCAGAAT+AGG	+	chr5.1:72172961-72172980	None:intergenic	35.0%
	AATCCAGATGAAACTGTAGA+AGG	-	chr5.1:72172523-72172542	MS.gene017053:CDS	35.0%
	AATCTATACCATATGCATGG+TGG	+	chr5.1:72172840-72172859	None:intergenic	35.0%
	AATGATAAGACCAATGGGTA+CGG	+	chr5.1:72172725-72172744	None:intergenic	35.0%
	ACATGTCATAAAGTGAAGGA+TGG	+	chr5.1:72173091-72173110	None:intergenic	35.0%
	ATCCAGATGAAACTGTAGAA+GGG	-	chr5.1:72172524-72172543	MS.gene017053:CDS	35.0%
	ATTTCTTTAGACCAGTCTCT+TGG	-	chr5.1:72173852-72173871	MS.gene017053:CDS	35.0%
	CAAACATGTTCCTTGTATCA+TGG	-	chr5.1:72172967-72172986	MS.gene017053:CDS	35.0%
	CTTGTTCCAAAGAAGAAACA+TGG	-	chr5.1:72172550-72172569	MS.gene017053:CDS	35.0%
	GTTTCTTATTATGCCTTTGC+TGG	-	chr5.1:72172391-72172410	MS.gene017053:CDS	35.0%
	TCTCAAGGGAATATTCGAAT+AGG	-	chr5.1:72172626-72172645	MS.gene017053:CDS	35.0%
	TGGTAGATTAACAAAAGAGC+TGG	-	chr5.1:72173457-72173476	MS.gene017053:intron	35.0%
	TTCTGCTTTCTTGTGGAAAA+TGG	-	chr5.1:72173286-72173305	MS.gene017053:intron	35.0%
	TTTCCACAAGAAAGCAGAAA+AGG	+	chr5.1:72173285-72173304	None:intergenic	35.0%
	TTTCTTGTGGAAAATGGTTG+CGG	-	chr5.1:72173292-72173311	MS.gene017053:intron	35.0%
	TTTCTTTAGACCAGTCTCTT+GGG	-	chr5.1:72173853-72173872	MS.gene017053:CDS	35.0%
!	AGATCGAGTTCATGAGTTTT+TGG	-	chr5.1:72173505-72173524	MS.gene017053:CDS	35.0%
!	CCCTTGAGAACAAAATGTTT+TGG	+	chr5.1:72172615-72172634	None:intergenic	35.0%
!	GGATCTTATCATTTTCCTTG+GGG	-	chr5.1:72173716-72173735	MS.gene017053:CDS	35.0%
!!!	TGGTTTAGTGGTGTTGTTTT+GGG	-	chr5.1:72172813-72172832	MS.gene017053:CDS	35.0%
!!!	TTGGTTTAGTGGTGTTGTTT+TGG	-	chr5.1:72172812-72172831	MS.gene017053:CDS	35.0%
	AATGTGCTTTCGATTCCCTA+TGG	-	chr5.1:72173434-72173453	MS.gene017053:intron	40.0%
	ACAACATGGATGAAATGTGG+TGG	-	chr5.1:72172894-72172913	MS.gene017053:CDS	40.0%
	ACTACCACCATTGTTTGAGT+TGG	+	chr5.1:72173240-72173259	None:intergenic	40.0%
	AGAAGAAGGACCAACATTTG+TGG	-	chr5.1:72173631-72173650	MS.gene017053:CDS	40.0%
	AGAAGTGACTGTCGAAAACA+AGG	+	chr5.1:72173044-72173063	None:intergenic	40.0%
	AGCACACCATGTTTCTTCTT+TGG	+	chr5.1:72172559-72172578	None:intergenic	40.0%
	AGGATTTATTGTGGTAGCAC+TGG	-	chr5.1:72173605-72173624	MS.gene017053:CDS	40.0%
	CAATAAATCCTTGCCAATCC+AGG	+	chr5.1:72173596-72173615	None:intergenic	40.0%
	CAGACAACATGGATGAAATG+TGG	-	chr5.1:72172891-72172910	MS.gene017053:CDS	40.0%
	CAGGGGGAAGAATTAAATCA+AGG	+	chr5.1:72172270-72172289	None:intergenic	40.0%
	CCAACATTTGTGGTATCTTC+AGG	-	chr5.1:72173641-72173660	MS.gene017053:CDS	40.0%
	CCTGAAGATACCACAAATGT+TGG	+	chr5.1:72173644-72173663	None:intergenic	40.0%
	CGATTGTTGCAAAGTAACTC+AGG	+	chr5.1:72172445-72172464	None:intergenic	40.0%
	CTTGGGTTTAATAGATTGGG+TGG	-	chr5.1:72173553-72173572	MS.gene017053:CDS	40.0%
	GAAAACAAGGTTGCGTTGTT+GGG	+	chr5.1:72173031-72173050	None:intergenic	40.0%
	GAAGTCGTTGCTAATTCCAT+GGG	-	chr5.1:72172415-72172434	MS.gene017053:CDS	40.0%
	GTCATAAAGTGAAGGATGGA+TGG	+	chr5.1:72173087-72173106	None:intergenic	40.0%
	TAAAGTGAAGGATGGATGGA+AGG	+	chr5.1:72173083-72173102	None:intergenic	40.0%
	TATGACCAACGAGGAAATAG+TGG	-	chr5.1:72172201-72172220	MS.gene017053:CDS	40.0%
	TCAAACATCCCACAATGCTA+TGG	+	chr5.1:72172348-72172367	None:intergenic	40.0%
	TCACTAGCCAACTCAAACAA+TGG	-	chr5.1:72173230-72173249	MS.gene017053:intron	40.0%
	TCTAAAGAAATGAGGAGCCT+GGG	+	chr5.1:72173843-72173862	None:intergenic	40.0%
	TGAAGTCGTTGCTAATTCCA+TGG	-	chr5.1:72172414-72172433	MS.gene017053:CDS	40.0%
	TGGGTGCCAGTAGTAATTAT+AGG	+	chr5.1:72172325-72172344	None:intergenic	40.0%
	TTCCCTTCTACAGTTTCATC+TGG	+	chr5.1:72172529-72172548	None:intergenic	40.0%
	TTGTGGTATCTTCAGGACAA+AGG	-	chr5.1:72173648-72173667	MS.gene017053:CDS	40.0%
	TTGTTCTTGTGCAGACAACA+TGG	-	chr5.1:72172880-72172899	MS.gene017053:CDS	40.0%
!	AACCAGTCACCATTCCTTTT+AGG	+	chr5.1:72173776-72173795	None:intergenic	40.0%
!	ACCAGTCACCATTCCTTTTA+GGG	+	chr5.1:72173775-72173794	None:intergenic	40.0%
!	AGTCCTTTTCTGCTTTCTTG+TGG	-	chr5.1:72173279-72173298	MS.gene017053:intron	40.0%
!	ATTTATTGTGGTAGCACTGG+TGG	-	chr5.1:72173608-72173627	MS.gene017053:CDS	40.0%
!	CACTTTTGACCATCGTATAG+CGG	-	chr5.1:72172932-72172951	MS.gene017053:CDS	40.0%
!	GAGTTACTTTGCAACAATCG+TGG	-	chr5.1:72172445-72172464	MS.gene017053:CDS	40.0%
!	GGATTGGCAAGGATTTATTG+TGG	-	chr5.1:72173596-72173615	MS.gene017053:CDS	40.0%
!	TCAGAGTCAAATTCAGGTTC+AGG	+	chr5.1:72173143-72173162	None:intergenic	40.0%
!	TCTTATCATTTTCCTTGGGG+AGG	-	chr5.1:72173719-72173738	MS.gene017053:CDS	40.0%
!!	AGTCGAAGGTTGATTTTGGA+TGG	-	chr5.1:72173678-72173697	MS.gene017053:CDS	40.0%
!!	AGTTCATGAGTTTTTGGAGG+CGG	-	chr5.1:72173511-72173530	MS.gene017053:CDS	40.0%
!!	GAGACTGGTCTAAAGAAATG+AGG	+	chr5.1:72173851-72173870	None:intergenic	40.0%
!!	GATGAAATGTGGTGGATTAG+TGG	-	chr5.1:72172902-72172921	MS.gene017053:CDS	40.0%
!!	GGTTGATAATCCGCTATGTT+GGG	-	chr5.1:72173478-72173497	MS.gene017053:CDS	40.0%
!!	GTCGAAGGTTGATTTTGGAT+GGG	-	chr5.1:72173679-72173698	MS.gene017053:CDS	40.0%
!!	TCGAAGGTTGATTTTGGATG+GGG	-	chr5.1:72173680-72173699	MS.gene017053:CDS	40.0%
!!	TCGAGTTCATGAGTTTTTGG+AGG	-	chr5.1:72173508-72173527	MS.gene017053:CDS	40.0%
!!	TGGTTGATAATCCGCTATGT+TGG	-	chr5.1:72173477-72173496	MS.gene017053:CDS	40.0%
	AAAATGGGGCTTGTGGTTGA+CGG	-	chr5.1:72173350-72173369	MS.gene017053:intron	45.0%
	AACATCCCACAATGCTATGG+TGG	+	chr5.1:72172345-72172364	None:intergenic	45.0%
	AAGAACACACCGACATCAAC+AGG	+	chr5.1:72172289-72172308	None:intergenic	45.0%
	AAGTAACTCAGGTTCACCCA+TGG	+	chr5.1:72172434-72172453	None:intergenic	45.0%
	AATAGGCATCCGCTATACGA+TGG	+	chr5.1:72172944-72172963	None:intergenic	45.0%
	ACAAAGGTTTCCAGAGTCGA+AGG	-	chr5.1:72173664-72173683	MS.gene017053:CDS	45.0%
	ACACCACTAAACCAACCAAG+TGG	+	chr5.1:72172807-72172826	None:intergenic	45.0%
	ACATCCCACAATGCTATGGT+GGG	+	chr5.1:72172344-72172363	None:intergenic	45.0%
	ACTTTGCAACAATCGTGGAG+TGG	-	chr5.1:72172450-72172469	MS.gene017053:CDS	45.0%
	AGAACACACCGACATCAACA+GGG	+	chr5.1:72172288-72172307	None:intergenic	45.0%
	ATCATTGGGCTGAGCCATTT+CGG	+	chr5.1:72172994-72173013	None:intergenic	45.0%
	ATGAAGTGCAAGTGTCCCTT+AGG	+	chr5.1:72173815-72173834	None:intergenic	45.0%
	ATGCCAAGCCCTAAAAGGAA+TGG	-	chr5.1:72173764-72173783	MS.gene017053:CDS	45.0%
	ATTCTTCCCCCTGTTGATGT+CGG	-	chr5.1:72172277-72172296	MS.gene017053:CDS	45.0%
	CAACGTGAATATGACCAACG+AGG	-	chr5.1:72172192-72172211	MS.gene017053:CDS	45.0%
	CCAAAATCAACCTTCGACTC+TGG	+	chr5.1:72173677-72173696	None:intergenic	45.0%
	CGAAAACAAGGTTGCGTTGT+TGG	+	chr5.1:72173032-72173051	None:intergenic	45.0%
	CTAGCCAACTCAAACAATGG+TGG	-	chr5.1:72173233-72173252	MS.gene017053:intron	45.0%
	CTCTACATGCACCTTCCTAA+GGG	-	chr5.1:72173797-72173816	MS.gene017053:CDS	45.0%
	GGAAGAAAGAGGCTAAGCAA+CGG	-	chr5.1:72173371-72173390	MS.gene017053:intron	45.0%
	GTCTAAAGAAATGAGGAGCC+TGG	+	chr5.1:72173844-72173863	None:intergenic	45.0%
	TAAGGGACACTTGCACTTCA+TGG	-	chr5.1:72173814-72173833	MS.gene017053:CDS	45.0%
	TGATAATCCGCTATGTTGGG+TGG	-	chr5.1:72173481-72173500	MS.gene017053:CDS	45.0%
	TGTTGCAAAAATGGGGCTTG+TGG	-	chr5.1:72173343-72173362	MS.gene017053:intron	45.0%
	TTGTGGTTGACGGAAGAAAG+AGG	-	chr5.1:72173360-72173379	MS.gene017053:intron	45.0%
!	GAGTCAAATTCAGGTTCAGG+TGG	+	chr5.1:72173140-72173159	None:intergenic	45.0%
!	TCAAATTCAGGTTCAGGTGG+TGG	+	chr5.1:72173137-72173156	None:intergenic	45.0%
!!	CCAGAGTCGAAGGTTGATTT+TGG	-	chr5.1:72173674-72173693	MS.gene017053:CDS	45.0%
!!	CGAAGGTTGATTTTGGATGG+GGG	-	chr5.1:72173681-72173700	MS.gene017053:CDS	45.0%
!!	GAAATGTGGTGGATTAGTGG+TGG	-	chr5.1:72172905-72172924	MS.gene017053:CDS	45.0%
!!	GAACCACTTGGTTGGTTTAG+TGG	-	chr5.1:72172801-72172820	MS.gene017053:CDS	45.0%
!!	GTGACAGAGGAGCATTTCTT+GGG	-	chr5.1:72173536-72173555	MS.gene017053:CDS	45.0%
!!!	TCACCATTCCTTTTAGGGCT+TGG	+	chr5.1:72173770-72173789	None:intergenic	45.0%
!!!	TTCCTTTTAGGGCTTGGCAT+TGG	+	chr5.1:72173764-72173783	None:intergenic	45.0%
!!!	TTTTGGGACCACCATGCATA+TGG	-	chr5.1:72172829-72172848	MS.gene017053:CDS	45.0%
	AACACACCGACATCAACAGG+GGG	+	chr5.1:72172286-72172305	None:intergenic	50.0%
	AGCAACGACTTCACCAGCAA+AGG	+	chr5.1:72172407-72172426	None:intergenic	50.0%
	AGCTGCCACTATTTCCTCGT+TGG	+	chr5.1:72172209-72172228	None:intergenic	50.0%
	AGGAGGTAATCCCAAGAGAC+TGG	+	chr5.1:72173866-72173885	None:intergenic	50.0%
	AGTGAAGGATGGATGGAAGG+TGG	+	chr5.1:72173080-72173099	None:intergenic	50.0%
	AGTGCAAGTGTCCCTTAGGA+AGG	+	chr5.1:72173811-72173830	None:intergenic	50.0%
	ATCCTTGCCAATCCAGGAAC+TGG	+	chr5.1:72173590-72173609	None:intergenic	50.0%
	CCATTTCGGCCCATGATACA+AGG	+	chr5.1:72172980-72172999	None:intergenic	50.0%
	CCTTGTATCATGGGCCGAAA+TGG	-	chr5.1:72172977-72172996	MS.gene017053:CDS	50.0%
	GAACACACCGACATCAACAG+GGG	+	chr5.1:72172287-72172306	None:intergenic	50.0%
	GCCCTAAAAGGAATGGTGAC+TGG	-	chr5.1:72173771-72173790	MS.gene017053:CDS	50.0%
	GCTCTACATGCACCTTCCTA+AGG	-	chr5.1:72173796-72173815	MS.gene017053:CDS	50.0%
	GGAAGCCAATGCTCTTGCAT+TGG	+	chr5.1:72172238-72172257	None:intergenic	50.0%
	GTGAAGGATGGATGGAAGGT+GGG	+	chr5.1:72173079-72173098	None:intergenic	50.0%
	GTGCTTTCGATTCCCTATGG+TGG	-	chr5.1:72173437-72173456	MS.gene017053:intron	50.0%
	TGCCAATGCCAAGCCCTAAA+AGG	-	chr5.1:72173759-72173778	MS.gene017053:CDS	50.0%
	TGGTGGAGGTAGATCAGAGA+CGG	+	chr5.1:72173120-72173139	None:intergenic	50.0%
!	AATTCAGGTTCAGGTGGTGG+AGG	+	chr5.1:72173134-72173153	None:intergenic	50.0%
!	CGCTACCAATGCAAGAGCAT+TGG	-	chr5.1:72172230-72172249	MS.gene017053:CDS	50.0%
!!	ACATGGTGTGCTTGCTGTTC+AGG	-	chr5.1:72172567-72172586	MS.gene017053:CDS	50.0%
!!	GGTGACAGAGGAGCATTTCT+TGG	-	chr5.1:72173535-72173554	MS.gene017053:CDS	50.0%
!!	TCATGAGTTTTTGGAGGCGG+CGG	-	chr5.1:72173514-72173533	MS.gene017053:CDS	50.0%
	CGATCTGCCACCCAACATAG+CGG	+	chr5.1:72173491-72173510	None:intergenic	55.0%
	GCATCGTCCAGTTCCTGGAT+TGG	-	chr5.1:72173580-72173599	MS.gene017053:CDS	55.0%
	GGCACCCACCATAGCATTGT+GGG	-	chr5.1:72172337-72172356	MS.gene017053:CDS	55.0%
	GGGACACTTGCACTTCATGG+AGG	-	chr5.1:72173817-72173836	MS.gene017053:CDS	55.0%
	GGTGGAGGTAGATCAGAGAC+GGG	+	chr5.1:72173119-72173138	None:intergenic	55.0%
	GTGGAGGTAGATCAGAGACG+GGG	+	chr5.1:72173118-72173137	None:intergenic	55.0%
	TGGCACCCACCATAGCATTG+TGG	-	chr5.1:72172336-72172355	MS.gene017053:CDS	55.0%
	TTTCCTTGGGGAGGTAGTGC+TGG	-	chr5.1:72173728-72173747	MS.gene017053:CDS	55.0%
!	GGATGGGGGAAGCATGTGTT+TGG	-	chr5.1:72173695-72173714	MS.gene017053:CDS	55.0%
!!	GAAGAGCATCGTCCAGTTCC+TGG	-	chr5.1:72173575-72173594	MS.gene017053:CDS	55.0%
!!	GGTAGCACTGGTGGAGAAGA+AGG	-	chr5.1:72173617-72173636	MS.gene017053:CDS	55.0%
!!	GTCCAGTTCCTGGATTGGCA+AGG	-	chr5.1:72173585-72173604	MS.gene017053:CDS	55.0%
!!!	GGACTCGAGTTTTGAGCGCT+TGG	+	chr5.1:72173264-72173283	None:intergenic	55.0%
	CTTGCACTTCATGGAGGCCC+AGG	-	chr5.1:72173823-72173842	MS.gene017053:CDS	60.0%
	GACAGGCACTCCGTACCCAT+TGG	-	chr5.1:72172712-72172731	MS.gene017053:CDS	60.0%
	TAGCCAGCACTACCTCCCCA+AGG	+	chr5.1:72173734-72173753	None:intergenic	60.0%
	TTTGGAGGCGGCGGTGACAG+AGG	-	chr5.1:72173523-72173542	MS.gene017053:CDS	65.0%
!	GATGGAAGGTGGGCGTCGTG+GGG	+	chr5.1:72173069-72173088	None:intergenic	65.0%
!	GGATGGAAGGTGGGCGTCGT+GGG	+	chr5.1:72173070-72173089	None:intergenic	65.0%
!	TGGATGGAAGGTGGGCGTCG+TGG	+	chr5.1:72173071-72173090	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	72172169	72173892	72172169	ID=MS.gene017053
chr5.1	mRNA	72172169	72173892	72172169	ID=MS.gene017053.t1;Parent=MS.gene017053
chr5.1	exon	72173473	72173892	72173473	ID=MS.gene017053.t1.exon1;Parent=MS.gene017053.t1
chr5.1	CDS	72173473	72173892	72173473	ID=cds.MS.gene017053.t1;Parent=MS.gene017053.t1
chr5.1	exon	72172169	72173167	72172169	ID=MS.gene017053.t1.exon2;Parent=MS.gene017053.t1
chr5.1	CDS	72172169	72173167	72172169	ID=cds.MS.gene017053.t1;Parent=MS.gene017053.t1

Gene Sequence

>MS.gene017053

ATGTGTATTGGAAAAGTAGAACTCAACGTGAATATGACCAACGAGGAAATAGTGGCAGCTGCGCTACCAATGCAAGAGCATTGGCTTCCACTATCAAACCTTGATTTAATTCTTCCCCCTGTTGATGTCGGTGTGTTCTTTTGCTACAATAAGCCTATAATTACTACTGGCACCCACCATAGCATTGTGGGATGTTTGAAAAATTCTTTAGCTAAGGCTCTTGTTTCTTATTATGCCTTTGCTGGTGAAGTCGTTGCTAATTCCATGGGTGAACCTGAGTTACTTTGCAACAATCGTGGAGTGGATTTTGTTGAAGCTTTTGCTGATGTTGAGTTACAATCTCTAAATTTGTATAATCCAGATGAAACTGTAGAAGGGAAGCTTGTTCCAAAGAAGAAACATGGTGTGCTTGCTGTTCAGGTGAAAAAAGATTTATTTTATTACCAAAACATTTTGTTCTCAAGGGAATATTCGAATAGGCAAAGAATTATTATAAGCATGTACTATTATAATAATCATTATAATTATTAGTACTTGTGTTTAGACAGGCACTCCGTACCCATTGGTCTTATCATTTTACTGCACTGATGCGAATAAATTACGAACAATAATTTTTATTAAATTTTAATTTTGAACCACTTGGTTGGTTTAGTGGTGTTGTTTTGGGACCACCATGCATATGGTATAGATTATAAAGTGTTTTAATCGTCATTGTTCTTGTGCAGACAACATGGATGAAATGTGGTGGATTAGTGGTGGCTTGCACTTTTGACCATCGTATAGCGGATGCCTATTCTGCAAACATGTTCCTTGTATCATGGGCCGAAATGGCTCAGCCCAATGATAACAAGTCTTTGATCCCAACAACGCAACCTTGTTTTCGACAGTCACTTCTAACCCCACGACGCCCACCTTCCATCCATCCTTCACTTTATGACATGTATGTCCCCGTCTCTGATCTACCTCCACCACCTGAACCTGAATTTGACTCTGATATTAAAACTAACCCAATAATAAGTCGCATATACTATGTAACAAGCAAAGAACTCAACAACATGCAATCACTAGCCAACTCAAACAATGGTGGTAGTTCCAAGCGCTCAAAACTCGAGTCCTTTTCTGCTTTCTTGTGGAAAATGGTTGCGGAAGCCGCTTCAATCGATAATGAAAACATTGTTGCAAAAATGGGGCTTGTGGTTGACGGAAGAAAGAGGCTAAGCAACGGTGACAAAAACAAAGAAGCGATTATGAATTCATATTTTGGGAATGTGCTTTCGATTCCCTATGGTGGTAGATTAACAAAAGAGCTGGTTGATAATCCGCTATGTTGGGTGGCAGATCGAGTTCATGAGTTTTTGGAGGCGGCGGTGACAGAGGAGCATTTCTTGGGTTTAATAGATTGGGTGGAAGAGCATCGTCCAGTTCCTGGATTGGCAAGGATTTATTGTGGTAGCACTGGTGGAGAAGAAGGACCAACATTTGTGGTATCTTCAGGACAAAGGTTTCCAGAGTCGAAGGTTGATTTTGGATGGGGGAAGCATGTGTTTGGATCTTATCATTTTCCTTGGGGAGGTAGTGCTGGCTATGTTATGCCAATGCCAAGCCCTAAAAGGAATGGTGACTGGTTGCTCTACATGCACCTTCCTAAGGGACACTTGCACTTCATGGAGGCCCAGGCTCCTCATTTCTTTAGACCAGTCTCTTGGGATTACCTCCTCAATTAA

Protein sequence

>MS.gene017053.t1

MCIGKVELNVNMTNEEIVAAALPMQEHWLPLSNLDLILPPVDVGVFFCYNKPIITTGTHHSIVGCLKNSLAKALVSYYAFAGEVVANSMGEPELLCNNRGVDFVEAFADVELQSLNLYNPDETVEGKLVPKKKHGVLAVQTTWMKCGGLVVACTFDHRIADAYSANMFLVSWAEMAQPNDNKSLIPTTQPCFRQSLLTPRRPPSIHPSLYDMYVPVSDLPPPPEPEFDSDIKTNPIISRIYYVTSKELNNMQSLANSNNGGSSKRSKLESFSAFLWKMVAEAASIDNENIVAKMGLVVDGRKRLSNGDKNKEAIMNSYFGNVLSIPYGGRLTKELVDNPLCWVADRVHEFLEAAVTEEHFLGLIDWVEEHRPVPGLARIYCGSTGGEEGPTFVVSSGQRFPESKVDFGWGKHVFGSYHFPWGGSAGYVMPMPSPKRNGDWLLYMHLPKGHLHFMEAQAPHFFRPVSWDYLLN