AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene019178

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019178.t1 MTR_3g450710 92.453 477 29 1 1 477 1 470 0.0 885
MS.gene019178.t1 MTR_3g450690 88.679 477 51 1 1 477 1 474 0.0 831
MS.gene019178.t1 MTR_5g090830 67.432 479 136 8 4 475 4 469 0.0 652
MS.gene019178.t1 MTR_5g090810 66.806 479 139 8 4 475 4 469 0.0 649
MS.gene019178.t1 MTR_5g090880 64.421 475 150 6 9 475 8 471 0.0 591
MS.gene019178.t1 MTR_5g026320 35.789 475 269 14 7 476 14 457 9.32e-77 248
MS.gene019178.t1 MTR_8g075610 25.225 444 271 15 33 460 16 414 1.89e-30 122
MS.gene019178.t1 MTR_4g097090 24.625 467 290 17 22 460 17 449 2.38e-26 111
MS.gene019178.t1 MTR_4g007540 25.586 469 290 18 9 460 1 427 3.37e-26 110
MS.gene019178.t1 MTR_8g096530 26.382 398 236 14 32 416 28 381 5.68e-25 107
MS.gene019178.t1 MTR_2g064495 24.099 444 279 16 33 460 25 426 3.13e-24 105
MS.gene019178.t1 MTR_4g127650 25.059 427 259 16 21 419 17 410 5.53e-24 104
MS.gene019178.t1 MTR_2g105330 24.099 444 279 16 33 460 25 426 7.04e-24 103
MS.gene019178.t1 MTR_3g049150 22.249 418 272 12 68 460 51 440 9.17e-23 100
MS.gene019178.t1 MTR_7g114520 24.757 412 261 12 72 467 66 444 6.33e-22 98.6
MS.gene019178.t1 MTR_1g053315 26.722 363 224 14 72 420 78 412 1.67e-21 97.4
MS.gene019178.t1 MTR_3g026950 22.788 452 296 13 32 460 23 444 3.19e-21 96.3
MS.gene019178.t1 MTR_7g027175 26.185 401 241 15 32 420 37 394 1.20e-20 94.7
MS.gene019178.t1 MTR_1g040175 25.236 424 238 17 72 461 64 442 1.34e-20 94.7
MS.gene019178.t1 MTR_3g098980 24.181 397 245 13 32 416 35 387 1.75e-20 94.0
MS.gene019178.t1 MTR_1g040215 25.500 400 228 15 72 439 64 425 2.63e-20 93.6
MS.gene019178.t1 MTR_7g114550 23.770 366 223 10 72 420 65 391 8.70e-20 92.0
MS.gene019178.t1 MTR_3g070170 25.304 494 275 21 22 468 20 466 1.81e-19 91.3
MS.gene019178.t1 MTR_5g076700 24.242 396 239 13 72 439 65 427 2.10e-19 90.9
MS.gene019178.t1 MTR_7g114560 23.288 365 232 10 69 420 62 391 2.25e-19 90.9
MS.gene019178.t1 MTR_7g086000 26.933 375 217 15 72 416 60 407 4.39e-19 90.1
MS.gene019178.t1 MTR_7g114470 23.441 401 237 15 72 439 66 429 1.50e-18 88.6
MS.gene019178.t1 MTR_1g081660 25.000 376 221 14 72 428 73 406 1.98e-18 87.8
MS.gene019178.t1 MTR_3g101930 24.866 374 228 13 66 418 64 405 9.64e-18 85.9
MS.gene019178.t1 MTR_7g114490 24.468 376 241 13 72 435 65 409 2.78e-17 84.7
MS.gene019178.t1 MTR_7g084940 27.019 359 212 14 72 416 69 391 5.64e-16 80.5
MS.gene019178.t1 MTR_3g465990 25.545 321 200 11 72 377 68 364 6.59e-16 80.1
MS.gene019178.t1 MTR_4g055170 24.397 373 217 13 72 418 69 402 2.18e-15 78.6
MS.gene019178.t1 MTR_3g086020 22.892 415 256 15 11 416 5 364 1.53e-14 75.9
MS.gene019178.t1 MTR_3g112180 24.221 417 245 13 23 418 24 390 3.07e-14 75.1
MS.gene019178.t1 MTR_8g079980 22.062 417 239 13 67 454 54 413 6.59e-14 73.9
MS.gene019178.t1 MTR_3g465980 24.299 321 204 11 72 377 68 364 7.20e-14 73.9
MS.gene019178.t1 MTR_6g053810 26.220 328 183 13 70 371 69 363 1.05e-13 73.6
MS.gene019178.t1 MTR_4g094842 24.297 391 241 16 46 418 45 398 7.29e-13 70.9
MS.gene019178.t1 MTR_7g084910 24.190 463 258 20 11 440 17 419 1.07e-12 70.1
MS.gene019178.t1 MTR_7g114510 22.018 327 215 8 148 467 9 302 2.03e-12 68.6
MS.gene019178.t1 MTR_8g041770 24.468 470 253 19 24 461 22 421 5.25e-12 68.2
MS.gene019178.t1 MTR_7g110610 28.571 154 98 4 72 213 55 208 1.42e-11 66.6
MS.gene019178.t1 MTR_2g007880 24.079 407 248 13 32 420 40 403 6.60e-11 64.7
MS.gene019178.t1 MTR_1g035890 26.522 230 150 5 72 294 67 284 8.00e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019178.t1 AT1G78990 56.688 471 174 7 9 476 11 454 0.0 514
MS.gene019178.t1 AT1G32910 56.962 474 171 7 9 475 11 458 4.03e-178 507
MS.gene019178.t1 AT5G16410 50.833 480 193 11 8 476 33 480 1.67e-158 458
MS.gene019178.t1 AT1G31490 36.307 482 249 15 9 476 4 441 8.00e-80 255
MS.gene019178.t1 AT5G48930 26.258 457 278 19 20 460 14 427 1.02e-26 112
MS.gene019178.t1 AT5G41040 26.341 410 231 16 32 420 54 413 1.64e-25 109
MS.gene019178.t1 AT5G41040 26.341 410 231 16 32 420 38 397 1.79e-25 108
MS.gene019178.t1 AT5G57840 26.585 489 284 21 11 476 3 439 2.48e-23 102
MS.gene019178.t1 AT3G03480 24.948 477 265 17 5 441 13 436 1.23e-22 100
MS.gene019178.t1 AT3G48720 25.591 465 287 16 9 460 7 425 3.99e-22 99.0
MS.gene019178.t1 AT2G19070 23.944 426 268 16 21 420 11 406 2.75e-21 96.7
MS.gene019178.t1 AT1G65450 26.005 373 213 14 72 427 72 398 8.03e-21 95.1
MS.gene019178.t1 AT1G28680 24.814 403 261 14 25 415 12 384 2.13e-19 90.9
MS.gene019178.t1 AT5G63560 25.635 433 253 17 6 420 2 383 8.33e-19 89.0
MS.gene019178.t1 AT5G17540 24.319 477 259 20 8 439 6 425 1.28e-18 88.6
MS.gene019178.t1 AT1G28680 24.934 377 248 13 25 387 12 367 1.33e-17 85.1
MS.gene019178.t1 AT1G24430 25.293 427 241 18 11 416 5 374 2.56e-17 84.3
MS.gene019178.t1 AT1G03390 24.818 411 253 16 27 420 38 409 2.61e-17 84.7
MS.gene019178.t1 AT5G01210 26.122 490 251 22 36 464 24 463 6.11e-17 83.6
MS.gene019178.t1 AT1G27620 24.838 463 272 20 18 460 16 422 1.11e-16 82.4
MS.gene019178.t1 AT3G50270 25.199 377 222 17 65 416 55 396 3.24e-16 81.3
MS.gene019178.t1 AT3G26040 24.609 447 237 20 11 422 3 384 3.27e-16 81.3
MS.gene019178.t1 AT5G67150 24.356 427 257 17 68 473 57 438 5.44e-15 77.4
MS.gene019178.t1 AT4G15390 25.688 327 193 12 30 343 29 318 1.58e-14 75.9
MS.gene019178.t1 AT4G15390 25.688 327 193 12 30 343 29 318 1.58e-14 75.9
MS.gene019178.t1 AT5G67160 24.711 433 251 18 62 473 46 424 1.87e-14 75.9
MS.gene019178.t1 AT3G50300 24.457 368 228 13 68 416 55 391 4.46e-14 74.7
MS.gene019178.t1 AT5G47950 23.264 288 179 7 7 287 2 254 1.24e-13 73.2
MS.gene019178.t1 AT3G30280 24.643 280 170 7 20 289 17 265 1.70e-13 72.8
MS.gene019178.t1 AT1G24420 22.418 397 244 15 83 464 76 423 4.01e-13 71.6
MS.gene019178.t1 AT1G24420 22.418 397 244 15 83 464 76 423 4.01e-13 71.6
MS.gene019178.t1 AT5G42830 22.529 435 270 17 62 470 54 447 9.45e-13 70.5
MS.gene019178.t1 AT5G07080 24.033 362 232 11 72 418 70 403 1.44e-12 70.1
MS.gene019178.t1 AT5G38130 23.980 392 235 16 67 434 74 426 2.10e-12 69.3
MS.gene019178.t1 AT2G25150 25.200 250 161 7 72 308 67 303 4.74e-12 68.2
MS.gene019178.t1 AT5G23970 22.951 427 252 16 15 421 7 376 6.07e-12 67.8

Find 155 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGAAGACAAGGTGGATTT+TGG 0.150775 3.3:-37162327 MS.gene019178:CDS
TGCCAATGCCAAGCCCTTTA+AGG 0.191687 3.3:-37162242 MS.gene019178:CDS
CTTATTAACCTTTGAATCTA+TGG 0.198337 3.3:-37165562 MS.gene019178:CDS
ATCAACCACTATGCCCATTT+TGG 0.201094 3.3:+37162656 None:intergenic
ACCCAATCACCATTCCTTAA+AGG 0.230515 3.3:+37162228 None:intergenic
TAAAATGGTTATTGCCAAAA+TGG 0.237738 3.3:-37162670 MS.gene019178:CDS
GTCTCATACTATGCCTTTGC+AGG 0.246462 3.3:-37165504 MS.gene019178:CDS
CATAGATTCAAAGGTTAATA+AGG 0.252094 3.3:+37165563 None:intergenic
GCAACGATGACACACTATTT+TGG 0.255431 3.3:-37162585 MS.gene019178:CDS
ACAACGGAGGAGCACTTCTT+AGG 0.274268 3.3:-37162459 MS.gene019178:CDS
GAAGGCATTGGCTAAGGTTT+TGG 0.280833 3.3:-37165526 MS.gene019178:CDS
AGCACGCCATGCTTCCTCTT+TGG 0.295076 3.3:+37165339 None:intergenic
CACAAAAGCCGGCCCTTCAT+TGG 0.296461 3.3:+37162371 None:intergenic
GTCTTCGGGGCAAAGATTTC+CGG 0.311488 3.3:-37162346 MS.gene019178:CDS
TTGGGCTTGATGGGAATTGT+TGG 0.311902 3.3:+37162993 None:intergenic
ACTTCTTAGGGCTCATTGAT+TGG 0.336465 3.3:-37162446 MS.gene019178:CDS
TTTCCTTGGGGTGGTGAAGC+TGG 0.337680 3.3:-37162273 MS.gene019178:CDS
CTTCACCACCCCAAGGAAAA+TGG 0.338457 3.3:+37162277 None:intergenic
ACAAAAGCCGGCCCTTCATT+GGG 0.339184 3.3:+37162372 None:intergenic
CACAAGGAACGGTAGTAAAA+TGG 0.339626 3.3:-37162685 MS.gene019178:CDS
GGCCGAAGGAGGAGGGAGTT+TGG 0.342738 3.3:+37162866 None:intergenic
TGTTATTGTGTTGGGCTTGA+TGG 0.358214 3.3:+37162983 None:intergenic
ATATCTAAGAAAATGGATAA+TGG 0.358864 3.3:-37165756 None:intergenic
CCCAATCACCATTCCTTAAA+GGG 0.360645 3.3:+37162229 None:intergenic
CTTCTTAGGGCTCATTGATT+GGG 0.361435 3.3:-37162445 MS.gene019178:CDS
CCAAAATCCACCTTGTCTTC+CGG 0.364145 3.3:+37162327 None:intergenic
CTCAGGTTCACCCACAGAAT+TGG 0.365871 3.3:+37165470 None:intergenic
GCCCTTTAAGGAATGGTGAT+TGG 0.369179 3.3:-37162230 MS.gene019178:CDS
AATGTTCTTTCTATCCCATT+TGG 0.374402 3.3:-37162561 MS.gene019178:CDS
GAGTTGGTTGATAAGCCATT+AGG 0.377668 3.3:-37162522 MS.gene019178:CDS
AAGAAAATGGATAATGGAAA+TGG 0.381443 3.3:-37165750 None:intergenic
CCCTTTAAGGAATGGTGATT+GGG 0.385492 3.3:-37162229 MS.gene019178:CDS
AGGAGGAGGGAGTTTGGAAA+TGG 0.390066 3.3:+37162872 None:intergenic
TGTCTTTATGCCTTTGACTT+GGG 0.398097 3.3:-37162148 MS.gene019178:CDS
TTTCCTTCAACAGATTCATC+AGG 0.405811 3.3:+37165369 None:intergenic
TGGGCTTGATGGGAATTGTT+GGG 0.406569 3.3:+37162994 None:intergenic
CAATAAATCTTCGCCAATCC+AGG 0.408088 3.3:+37162402 None:intergenic
CTACGGTGACGTAGTATATT+CGG 0.410471 3.3:+37162820 None:intergenic
CTCAAGCTTCGAAGGCTTAG+TGG 0.414101 3.3:+37162749 None:intergenic
TCCACTGATTTACCGCCAAA+TGG 0.414799 3.3:+37162546 None:intergenic
AGTTGGTTGATAAGCCATTA+GGG 0.417113 3.3:-37162521 MS.gene019178:CDS
GGCAACCAATGTTCTTGTAT+TGG 0.419540 3.3:+37165675 None:intergenic
TCACCATTCCTTAAAGGGCT+TGG 0.420562 3.3:+37162234 None:intergenic
GGAGGAGGGAGTTTGGAAAT+GGG 0.420619 3.3:+37162873 None:intergenic
TTCCAAACTCCCTCCTCCTT+CGG 0.432214 3.3:-37162868 MS.gene019178:CDS
ATGTCTTTATGCCTTTGACT+TGG 0.436128 3.3:-37162149 MS.gene019178:CDS
GTTATTGTGTTGGGCTTGAT+GGG 0.437078 3.3:+37162984 None:intergenic
AATTGTTGGGTGGGCTACCT+CGG 0.438525 3.3:+37163007 None:intergenic
TCATCAACCACCCACCCTAA+TGG 0.439188 3.3:+37162507 None:intergenic
CAAACATGTTCCTAGTATCA+TGG 0.440376 3.3:-37163031 MS.gene019178:CDS
TTAGCGGTTGTTATTGTGTT+GGG 0.440768 3.3:+37162975 None:intergenic
TATTGTGGTGGCCCCAATGA+AGG 0.441633 3.3:-37162384 MS.gene019178:CDS
AACCCTGATGAATCTGTTGA+AGG 0.446103 3.3:-37165372 MS.gene019178:CDS
AAAATGGTTATTGCCAAAAT+GGG 0.451609 3.3:-37162669 MS.gene019178:CDS
CTACCTCGGCCCATGATACT+AGG 0.452304 3.3:+37163021 None:intergenic
TTCCTTAAAGGGCTTGGCAT+TGG 0.454305 3.3:+37162240 None:intergenic
GCATGGCGTGCTCGCAGTCC+AGG 0.455368 3.3:-37165328 MS.gene019178:intron
TTCCGCCTACTTGTGGAAGT+TGG 0.467896 3.3:-37162721 MS.gene019178:CDS
CTTCTACCTGCAGTGGATGT+TGG 0.468221 3.3:-37165633 MS.gene019178:CDS
CCCGAAGACACCACAAAAGC+CGG 0.468680 3.3:+37162360 None:intergenic
CAACGGAGGAGCACTTCTTA+GGG 0.471002 3.3:-37162458 MS.gene019178:CDS
TGTTGAAGTGGACAATATCA+TGG 0.472306 3.3:+37165587 None:intergenic
GTTAGCGGTTGTTATTGTGT+TGG 0.485313 3.3:+37162974 None:intergenic
GTAGCGGTGGCCGAAGGAGG+AGG 0.486790 3.3:+37162858 None:intergenic
ACACCGACCTGTCCCTGGAT+TGG 0.493701 3.3:-37162415 MS.gene019178:CDS
CCACTGATTTACCGCCAAAT+GGG 0.498704 3.3:+37162547 None:intergenic
GAAGCACACCGACCTGTCCC+TGG 0.499177 3.3:-37162420 MS.gene019178:CDS
GTTCTTTCTATCCCATTTGG+CGG 0.503798 3.3:-37162558 MS.gene019178:CDS
AGGGAAGGGTTGATGAAACT+TGG 0.504037 3.3:+37162912 None:intergenic
GTGTCATCGTTGCTTCTCTC+CGG 0.504812 3.3:+37162595 None:intergenic
CAGCGAGTGATTGCAAGCGT+TGG 0.508815 3.3:+37162790 None:intergenic
GGTTAATAAGGTTGTTGAAG+TGG 0.511067 3.3:+37165575 None:intergenic
TCTCAAGAAGGCATTGGCTA+AGG 0.515499 3.3:-37165532 MS.gene019178:CDS
GGGATTATCTCAATCAATGA+TGG 0.518179 3.3:-37162128 None:intergenic
ATTAGGGTGGGTGGTTGATG+AGG 0.520603 3.3:-37162505 MS.gene019178:CDS
ACGATAGTAGCGATGAGGAC+CGG 0.521532 3.3:-37162614 MS.gene019178:CDS
ATCTTCGCCAATCCAGGGAC+AGG 0.521874 3.3:+37162408 None:intergenic
CGGTAAATCAGTGGAGGAGT+TGG 0.526547 3.3:-37162538 MS.gene019178:CDS
AAAATGGGCATAGTGGTTGA+TGG 0.529328 3.3:-37162654 MS.gene019178:CDS
CTTGATGGGAATTGTTGGGT+GGG 0.533436 3.3:+37162998 None:intergenic
TGGTGGCCCCAATGAAGGGC+CGG 0.537013 3.3:-37162379 MS.gene019178:CDS
CGGTTATTACAAAGAAGCTC+AGG 0.537800 3.3:+37165453 None:intergenic
GTTGATGGAAGAAGGAGACT+TGG 0.539963 3.3:-37162639 MS.gene019178:CDS
TGATAAGCCATTAGGGTGGG+TGG 0.542648 3.3:-37162514 MS.gene019178:CDS
TTTGTAGCGGTGGCCGAAGG+AGG 0.544011 3.3:+37162855 None:intergenic
TGATTTACTTCTACCTGCAG+TGG 0.544886 3.3:-37165640 MS.gene019178:CDS
GCATAGTGGTTGATGGAAGA+AGG 0.547976 3.3:-37162647 MS.gene019178:CDS
TGAAGTTGTGTCCAATTCTG+TGG 0.548471 3.3:-37165481 MS.gene019178:CDS
GGCTTTGTAGCGGTGGCCGA+AGG 0.553032 3.3:+37162852 None:intergenic
AGACACAACCATAGATTCAA+AGG 0.553429 3.3:+37165554 None:intergenic
TAGTATATTCGGCTAAGTAA+CGG 0.554793 3.3:+37162831 None:intergenic
CAAGCGTTGGAGCTTCTCTA+CGG 0.561371 3.3:+37162803 None:intergenic
TTGGATGACGAGGGCTCAGG+AGG 0.565630 3.3:+37162931 None:intergenic
GGACACAACTTCACCTGCAA+AGG 0.565845 3.3:+37165491 None:intergenic
AAACATGTTCCTAGTATCAT+GGG 0.565848 3.3:-37163030 MS.gene019178:CDS
ATTGTGGTGGCCCCAATGAA+GGG 0.566085 3.3:-37162383 MS.gene019178:CDS
GTTGAAGTGGACAATATCAT+GGG 0.567565 3.3:+37165588 None:intergenic
ACGAAAACATGGTTTGTTAG+CGG 0.570643 3.3:+37162959 None:intergenic
CTGCAGGTAGAAGTAAATCA+AGG 0.571413 3.3:+37165643 None:intergenic
TATGGTTGTGTCTCTCAAGA+AGG 0.571432 3.3:-37165544 MS.gene019178:CDS
AGGAGGGAGCGACGAAAACA+TGG 0.572501 3.3:+37162948 None:intergenic
GCTTGATGGGAATTGTTGGG+TGG 0.576660 3.3:+37162997 None:intergenic
TTTGTTCCAAAGAGGAAGCA+TGG 0.576773 3.3:-37165345 MS.gene019178:CDS
CTTAGGGCTCATTGATTGGG+TGG 0.576867 3.3:-37162442 MS.gene019178:CDS
CCCATTTGGCGGTAAATCAG+TGG 0.577850 3.3:-37162547 MS.gene019178:CDS
TTCCTTCAACAGATTCATCA+GGG 0.578977 3.3:+37165370 None:intergenic
AAGGAAAGTTTGTTCCAAAG+AGG 0.579643 3.3:-37165353 MS.gene019178:CDS
TATTGCCAAAATGGGCATAG+TGG 0.579804 3.3:-37162661 MS.gene019178:CDS
TAGCGGTGGCCGAAGGAGGA+GGG 0.580998 3.3:+37162859 None:intergenic
TGTGTCTCTCAAGAAGGCAT+TGG 0.582810 3.3:-37165538 MS.gene019178:CDS
ATGCCAAGCCCTTTAAGGAA+TGG 0.583943 3.3:-37162237 MS.gene019178:CDS
AACTTGGATGACGAGGGCTC+AGG 0.585088 3.3:+37162928 None:intergenic
TGGGAATGTACATATCGTCA+AGG 0.591885 3.3:+37162892 None:intergenic
GCAAAGATTTCCGGAAGACA+AGG 0.593195 3.3:-37162337 MS.gene019178:CDS
AACACACCAACATCCACTGC+AGG 0.594572 3.3:+37165627 None:intergenic
TTGAGATAATCCCAAGTCAA+AGG 0.597274 3.3:+37162138 None:intergenic
GCTAAGTAACGGCTTTGTAG+CGG 0.597665 3.3:+37162842 None:intergenic
TGTGACAAATGAAGAGGTAG+TGG 0.598358 3.3:-37165709 MS.gene019178:CDS
GTTCCTAGTATCATGGGCCG+AGG 0.600763 3.3:-37163024 MS.gene019178:CDS
GCTAATGCAGCCACAAGGAA+CGG 0.601773 3.3:-37162696 MS.gene019178:CDS
GGTTGATAAGCCATTAGGGT+GGG 0.604220 3.3:-37162517 MS.gene019178:CDS
TGTACATATCGTCAAGGGAA+GGG 0.609748 3.3:+37162898 None:intergenic
CAAAAGCCGGCCCTTCATTG+GGG 0.611698 3.3:+37162373 None:intergenic
GGGAATGTACATATCGTCAA+GGG 0.612337 3.3:+37162893 None:intergenic
TGTTACCAATACAAGAACAT+TGG 0.612898 3.3:-37165680 MS.gene019178:CDS
AATAAATCTTCGCCAATCCA+GGG 0.616604 3.3:+37162403 None:intergenic
CTCGCTGCTTTAGCTAACAA+TGG 0.616670 3.3:-37162774 MS.gene019178:CDS
AGTAAATCAAGGTTAGAGAG+TGG 0.617047 3.3:+37165654 None:intergenic
GCAACTTCATTAAAGTGTGG+TGG 0.618452 3.3:-37163104 MS.gene019178:CDS
AAGTAACGGCTTTGTAGCGG+TGG 0.620157 3.3:+37162845 None:intergenic
ATTGGAGATCATAGAGTGCG+AGG 0.622406 3.3:-37162178 MS.gene019178:CDS
TTGGCGAAGATTTATTGTGG+TGG 0.627427 3.3:-37162396 MS.gene019178:CDS
AAAGTGTGGTGGAATAGTAG+TGG 0.632747 3.3:-37163093 MS.gene019178:CDS
GGATTGGCGAAGATTTATTG+TGG 0.634312 3.3:-37162399 MS.gene019178:CDS
TTGATGAAACTTGGATGACG+AGG 0.635264 3.3:+37162921 None:intergenic
ATGTACATATCGTCAAGGGA+AGG 0.640262 3.3:+37162897 None:intergenic
AAGATTTCCGGAAGACAAGG+TGG 0.642050 3.3:-37162334 MS.gene019178:CDS
TCGCCAATCCAGGGACAGGT+CGG 0.647662 3.3:+37162412 None:intergenic
TGGTTGATAAGCCATTAGGG+TGG 0.648057 3.3:-37162518 MS.gene019178:CDS
ACCACCCCAAGGAAAATGGT+AGG 0.648109 3.3:+37162281 None:intergenic
GTTTGTGTCAGTAGCAACAA+CGG 0.654514 3.3:-37162475 MS.gene019178:CDS
GAAGTTGTGTCCAATTCTGT+GGG 0.658652 3.3:-37165480 MS.gene019178:CDS
AACCAACTTCCACAAGTAGG+CGG 0.658696 3.3:+37162719 None:intergenic
AGCAACCAACTTCCACAAGT+AGG 0.662472 3.3:+37162716 None:intergenic
TGTGAGTGTGACAAATGAAG+AGG 0.662729 3.3:-37165715 MS.gene019178:CDS
TAGCCAGCTTCACCACCCCA+AGG 0.673339 3.3:+37162270 None:intergenic
TGGATGACGAGGGCTCAGGA+GGG 0.673717 3.3:+37162932 None:intergenic
CAGGCAACTTCATTAAAGTG+TGG 0.676025 3.3:-37163107 MS.gene019178:CDS
TGGTTGCTAATGCAGCCACA+AGG 0.676894 3.3:-37162701 MS.gene019178:CDS
ATTTGGCGGTAAATCAGTGG+AGG 0.680515 3.3:-37162544 MS.gene019178:CDS
GAGCTTCTTTGTAATAACCG+TGG 0.683740 3.3:-37165450 MS.gene019178:CDS
TGATGAAACTTGGATGACGA+GGG 0.690763 3.3:+37162922 None:intergenic
TGGAAACGATAGTAGCGATG+AGG 0.708341 3.3:-37162619 MS.gene019178:CDS
CTTCAACAAAATCAACACCA+CGG 0.742176 3.3:+37165433 None:intergenic
TGTGTCAGTAGCAACAACGG+AGG 0.765856 3.3:-37162472 MS.gene019178:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATTATAACCTAATT+TGG + chr3.3:37163256-37163275 None:intergenic 10.0%
!! AAATAATTATAACCTAATTT+GGG + chr3.3:37163255-37163274 None:intergenic 10.0%
!! AGAAAAAAGAAAAAAAAAAA+AGG + chr3.3:37163955-37163974 None:intergenic 10.0%
!! GAAAAAAGAAAAAAAAAAAA+GGG + chr3.3:37163954-37163973 None:intergenic 10.0%
!! TTATTCAAAATATTAGATAA+AGG + chr3.3:37163360-37163379 None:intergenic 10.0%
!!! ATAAAGGATTTTTTTTATAT+AGG + chr3.3:37163118-37163137 None:intergenic 10.0%
!!! TTGATTGAATATATTTTATT+CGG - chr3.3:37162597-37162616 MS.gene019178:CDS 10.0%
!! AAAAAAGTAATAAAAGTTCT+AGG + chr3.3:37162880-37162899 None:intergenic 15.0%
!! AAAAAGTAATAAAAGTTCTA+GGG + chr3.3:37162879-37162898 None:intergenic 15.0%
!! TTATTCAGAATATTAGATAA+AGG + chr3.3:37163134-37163153 None:intergenic 15.0%
!!! GAATGTTATAATTTTATTTG+AGG - chr3.3:37163197-37163216 MS.gene019178:intron 15.0%
!! ACCATTAAAAAATTTCAATG+TGG - chr3.3:37162759-37162778 MS.gene019178:CDS 20.0%
!! ATCATTCTTATATAAGCTTA+AGG + chr3.3:37163737-37163756 None:intergenic 20.0%
!! ATTAGAGCAAAAAGAAATAA+TGG + chr3.3:37164563-37164582 None:intergenic 20.0%
!! GAAAATAATCTGACATAATA+TGG + chr3.3:37164650-37164669 None:intergenic 20.0%
!! TATTTGTATTCTCATACATT+TGG - chr3.3:37164440-37164459 MS.gene019178:intron 20.0%
!! TGAAGAGGATAAAAAATAAA+AGG - chr3.3:37164474-37164493 MS.gene019178:intron 20.0%
!! TTAAGCTTATATAAGAATGA+TGG - chr3.3:37163736-37163755 MS.gene019178:intron 20.0%
!!! ATTGACTTTATTTTTTATGC+AGG - chr3.3:37164749-37164768 MS.gene019178:intron 20.0%
!!! TCCACATTGAAATTTTTTAA+TGG + chr3.3:37162763-37162782 None:intergenic 20.0%
!!! TTAGTATACTTTTAAGTCAT+CGG + chr3.3:37163489-37163508 None:intergenic 20.0%
!!! TTATAATTTTATTTGAGGTC+AGG - chr3.3:37163202-37163221 MS.gene019178:intron 20.0%
!!! TTTTGAATAACTGTAATAGA+TGG - chr3.3:37162668-37162687 MS.gene019178:CDS 20.0%
!!! TTTTTTATATAGGCAGATAA+AGG + chr3.3:37163108-37163127 None:intergenic 20.0%
! AAAATGGTTATTGCCAAAAT+GGG - chr3.3:37165206-37165225 MS.gene019178:intron 25.0%
! ATTCTTATATAAGCTTAAGG+TGG + chr3.3:37163734-37163753 None:intergenic 25.0%
! CATAGATTCAAAGGTTAATA+AGG + chr3.3:37162315-37162334 None:intergenic 25.0%
! CCAATTGATTATAAGATGAA+TGG - chr3.3:37164521-37164540 MS.gene019178:intron 25.0%
! CCATTCATCTTATAATCAAT+TGG + chr3.3:37164524-37164543 None:intergenic 25.0%
! CTTATTAACCTTTGAATCTA+TGG - chr3.3:37162313-37162332 MS.gene019178:CDS 25.0%
! TAAAATGGTTATTGCCAAAA+TGG - chr3.3:37165205-37165224 MS.gene019178:intron 25.0%
! TAATATGGCTACCATAAAAT+TGG + chr3.3:37164635-37164654 None:intergenic 25.0%
! TGGCAAAAAATTATTCTATC+AGG - chr3.3:37162976-37162995 MS.gene019178:CDS 25.0%
! TTCTTATATAAGCTTAAGGT+GGG + chr3.3:37163733-37163752 None:intergenic 25.0%
!! CTTTTTGCTCTAATTAAGAA+TGG - chr3.3:37164569-37164588 MS.gene019178:intron 25.0%
!! TTTCAATTTTTGTAACGTAG+TGG + chr3.3:37163557-37163576 None:intergenic 25.0%
!!! AATTAAGAATGGATTGGATA+CGG - chr3.3:37164580-37164599 MS.gene019178:intron 25.0%
!!! TACCATAAAATTGGCTTTAA+GGG + chr3.3:37164626-37164645 None:intergenic 25.0%
!!! TAGTATACTTTTAAGTCATC+GGG + chr3.3:37163488-37163507 None:intergenic 25.0%
!!! TCACATTGATTTTTGCTTAT+TGG + chr3.3:37164053-37164072 None:intergenic 25.0%
!!! TTGGTAATGAATAAATGAAG+AGG - chr3.3:37164459-37164478 MS.gene019178:intron 25.0%
AAACATGTTCCTAGTATCAT+GGG - chr3.3:37164845-37164864 MS.gene019178:intron 30.0%
AATAGTCACTACTTGAATAG+TGG - chr3.3:37163759-37163778 MS.gene019178:intron 30.0%
AATGTTCTTTCTATCCCATT+TGG - chr3.3:37165314-37165333 MS.gene019178:intron 30.0%
AGACGTCATTGAAATTTGAT+TGG - chr3.3:37164111-37164130 MS.gene019178:intron 30.0%
ATTATCCAACCCAATCAAAT+AGG + chr3.3:37164124-37164143 None:intergenic 30.0%
ATTGAAAGGTCAGTAAAACT+TGG - chr3.3:37162956-37162975 MS.gene019178:CDS 30.0%
CATCAGGGTTATAAAAATTG+AGG + chr3.3:37162493-37162512 None:intergenic 30.0%
GACGTCATTGAAATTTGATT+GGG - chr3.3:37164112-37164131 MS.gene019178:intron 30.0%
TAATCGAGAGTTCAATTGAA+AGG - chr3.3:37162942-37162961 MS.gene019178:CDS 30.0%
TAGTATATTCGGCTAAGTAA+CGG + chr3.3:37165047-37165066 None:intergenic 30.0%
TCATTGAAATTTGATTGGGT+TGG - chr3.3:37164116-37164135 MS.gene019178:intron 30.0%
TGCTCTAATTAAGAATGGAT+TGG - chr3.3:37164574-37164593 MS.gene019178:intron 30.0%
TGTTACCAATACAAGAACAT+TGG - chr3.3:37162195-37162214 MS.gene019178:CDS 30.0%
TTATCCAACCCAATCAAATA+GGG + chr3.3:37164180-37164199 None:intergenic 30.0%
! AGAGCTTGGAAAATTTTACA+GGG + chr3.3:37162830-37162849 None:intergenic 30.0%
! ATGGTATTGGACATTTGAAA+TGG - chr3.3:37163838-37163857 MS.gene019178:intron 30.0%
! TTGGACATTTGAAATGGATA+TGG - chr3.3:37163844-37163863 MS.gene019178:intron 30.0%
! TTTTGTGAGATAGGATACAA+AGG - chr3.3:37163918-37163937 MS.gene019178:intron 30.0%
!! AAGTAATAAAAGTTCTAGGG+TGG + chr3.3:37162876-37162895 None:intergenic 30.0%
!! CTACCATAAAATTGGCTTTA+AGG + chr3.3:37164627-37164646 None:intergenic 30.0%
!! TACAAAAGTCTTGTTTCTCT+AGG + chr3.3:37162924-37162943 None:intergenic 30.0%
!! TGTGTCGTTTAAATGTAACT+CGG + chr3.3:37164346-37164365 None:intergenic 30.0%
!! TTGATTGGGTTGGATAATAA+TGG - chr3.3:37164126-37164145 MS.gene019178:intron 30.0%
!! TTTTAAGTGTGGAAAATTCG+AGG - chr3.3:37164275-37164294 MS.gene019178:intron 30.0%
!!! TCATGTTAGTTTTGTGAGAT+AGG - chr3.3:37163909-37163928 MS.gene019178:intron 30.0%
AAAACTAAGGGGTGACAAAA+TGG - chr3.3:37162573-37162592 MS.gene019178:CDS 35.0%
AAAGGCAAAATGGATGGTAT+TGG - chr3.3:37163825-37163844 MS.gene019178:intron 35.0%
AATAAATCTTCGCCAATCCA+GGG + chr3.3:37165475-37165494 None:intergenic 35.0%
AATCTCTTACACATAACCCA+TGG + chr3.3:37163664-37163683 None:intergenic 35.0%
AGACACAACCATAGATTCAA+AGG + chr3.3:37162324-37162343 None:intergenic 35.0%
AGTAAATCAAGGTTAGAGAG+TGG + chr3.3:37162224-37162243 None:intergenic 35.0%
AGTCCAGGTATAAAACTAAG+GGG - chr3.3:37162562-37162581 MS.gene019178:CDS 35.0%
ATGTCTTTATGCCTTTGACT+TGG - chr3.3:37165726-37165745 MS.gene019178:CDS 35.0%
CAAACATGTTCCTAGTATCA+TGG - chr3.3:37164844-37164863 MS.gene019178:intron 35.0%
CAGTCCAGGTATAAAACTAA+GGG - chr3.3:37162561-37162580 MS.gene019178:CDS 35.0%
CTTCAACAAAATCAACACCA+CGG + chr3.3:37162445-37162464 None:intergenic 35.0%
GAGATTATTCCACTGTCTAT+TGG - chr3.3:37163678-37163697 MS.gene019178:intron 35.0%
GGTTAATAAGGTTGTTGAAG+TGG + chr3.3:37162303-37162322 None:intergenic 35.0%
GTTGAAGTGGACAATATCAT+GGG + chr3.3:37162290-37162309 None:intergenic 35.0%
TAGTGGCAATTAACTGTCAT+TGG + chr3.3:37163540-37163559 None:intergenic 35.0%
TATCCAACCCAATCAAATAG+GGG + chr3.3:37164179-37164198 None:intergenic 35.0%
TCGTTTAAATGTAACTCGGA+TGG + chr3.3:37164342-37164361 None:intergenic 35.0%
TGCATTTGAGTGCTTATCAA+TGG + chr3.3:37163883-37163902 None:intergenic 35.0%
TGTTGAAGTGGACAATATCA+TGG + chr3.3:37162291-37162310 None:intergenic 35.0%
TTCCTTCAACAGATTCATCA+GGG + chr3.3:37162508-37162527 None:intergenic 35.0%
TTGAGATAATCCCAAGTCAA+AGG + chr3.3:37165740-37165759 None:intergenic 35.0%
TTTCCTTCAACAGATTCATC+AGG + chr3.3:37162509-37162528 None:intergenic 35.0%
! AAGGAAAGTTTGTTCCAAAG+AGG - chr3.3:37162522-37162541 MS.gene019178:CDS 35.0%
! ATAATGGAGTTGGCCAAAAT+TGG - chr3.3:37164142-37164161 MS.gene019178:intron 35.0%
! ATGGATATGGACTGTGATAT+AGG - chr3.3:37163857-37163876 MS.gene019178:intron 35.0%
! CACCCTTAAAGCCAATTTTA+TGG - chr3.3:37164621-37164640 MS.gene019178:intron 35.0%
! CAGAGCTTGGAAAATTTTAC+AGG + chr3.3:37162831-37162850 None:intergenic 35.0%
! GAGCTTGGAAAATTTTACAG+GGG + chr3.3:37162829-37162848 None:intergenic 35.0%
! TGTCTTTATGCCTTTGACTT+GGG - chr3.3:37165727-37165746 MS.gene019178:CDS 35.0%
! TTAGCGGTTGTTATTGTGTT+GGG + chr3.3:37164903-37164922 None:intergenic 35.0%
! TTTATTTGAGGTCAGGAGTT+GGG - chr3.3:37163209-37163228 MS.gene019178:intron 35.0%
! TTTTGTGAGATAGGCTACAA+CGG + chr3.3:37163598-37163617 None:intergenic 35.0%
!! ACGAAAACATGGTTTGTTAG+CGG + chr3.3:37164919-37164938 None:intergenic 35.0%
!! AGACTCACACATTTTAAGTG+TGG - chr3.3:37164264-37164283 MS.gene019178:intron 35.0%
!! AGTTGGTTGATAAGCCATTA+GGG - chr3.3:37165354-37165373 MS.gene019178:CDS 35.0%
!! CATAAAATTGGCTTTAAGGG+TGG + chr3.3:37164623-37164642 None:intergenic 35.0%
!! GCATCTTTTGAAAGAGCAAT+TGG - chr3.3:37165679-37165698 MS.gene019178:CDS 35.0%
!! TTTTATTTGAGGTCAGGAGT+TGG - chr3.3:37163208-37163227 MS.gene019178:intron 35.0%
!!! GGGGTATTGGTTTTTTTTTG+AGG - chr3.3:37164025-37164044 MS.gene019178:intron 35.0%
AAAATGGGCATAGTGGTTGA+TGG - chr3.3:37165221-37165240 MS.gene019178:intron 40.0%
AACCCTGATGAATCTGTTGA+AGG - chr3.3:37162503-37162522 MS.gene019178:CDS 40.0%
AACCTGAACCCCTATTTGAT+TGG - chr3.3:37164168-37164187 MS.gene019178:intron 40.0%
AACGACAATTAACTGCTGCT+AGG - chr3.3:37164004-37164023 MS.gene019178:intron 40.0%
AATCAAATAGGGGTTCAGGT+TGG + chr3.3:37164169-37164188 None:intergenic 40.0%
ACCCAATCACCATTCCTTAA+AGG + chr3.3:37165650-37165669 None:intergenic 40.0%
ACCTGAACCCCTATTTGATT+GGG - chr3.3:37164169-37164188 MS.gene019178:intron 40.0%
ACCTTGTATCCAATAGACAG+TGG + chr3.3:37163690-37163709 None:intergenic 40.0%
ACGACAATTAACTGCTGCTA+GGG - chr3.3:37164005-37164024 MS.gene019178:intron 40.0%
ACTTCTTAGGGCTCATTGAT+TGG - chr3.3:37165429-37165448 MS.gene019178:CDS 40.0%
ATCATACAGGCTACAACCAT+GGG - chr3.3:37163645-37163664 MS.gene019178:intron 40.0%
ATGTACATATCGTCAAGGGA+AGG + chr3.3:37164981-37165000 None:intergenic 40.0%
CAATAAATCTTCGCCAATCC+AGG + chr3.3:37165476-37165495 None:intergenic 40.0%
CACAAGGAACGGTAGTAAAA+TGG - chr3.3:37165190-37165209 MS.gene019178:intron 40.0%
CAGGCAACTTCATTAAAGTG+TGG - chr3.3:37164768-37164787 MS.gene019178:intron 40.0%
CCCAATCACCATTCCTTAAA+GGG + chr3.3:37165649-37165668 None:intergenic 40.0%
CGGTTATTACAAAGAAGCTC+AGG + chr3.3:37162425-37162444 None:intergenic 40.0%
CTACGGTGACGTAGTATATT+CGG + chr3.3:37165058-37165077 None:intergenic 40.0%
CTGCAGGTAGAAGTAAATCA+AGG + chr3.3:37162235-37162254 None:intergenic 40.0%
CTTAAGGTGGGTCATCTAAA+TGG + chr3.3:37163721-37163740 None:intergenic 40.0%
GAAAAAGACTCAAGCTTCGA+AGG + chr3.3:37165137-37165156 None:intergenic 40.0%
GAAGTTGTGTCCAATTCTGT+GGG - chr3.3:37162395-37162414 MS.gene019178:CDS 40.0%
GAGCTTCTTTGTAATAACCG+TGG - chr3.3:37162425-37162444 MS.gene019178:CDS 40.0%
GATGCAAAAGGCAAAATGGA+TGG - chr3.3:37163819-37163838 MS.gene019178:intron 40.0%
GCAACTTCATTAAAGTGTGG+TGG - chr3.3:37164771-37164790 MS.gene019178:intron 40.0%
GCAGTCCAGGTATAAAACTA+AGG - chr3.3:37162560-37162579 MS.gene019178:CDS 40.0%
GGCAACCAATGTTCTTGTAT+TGG + chr3.3:37162203-37162222 None:intergenic 40.0%
GGGAATGTACATATCGTCAA+GGG + chr3.3:37164985-37165004 None:intergenic 40.0%
GGTAGATGCAAAAGGCAAAA+TGG - chr3.3:37163815-37163834 MS.gene019178:intron 40.0%
GTTATTGTGTTGGGCTTGAT+GGG + chr3.3:37164894-37164913 None:intergenic 40.0%
GTTCTTTCTATCCCATTTGG+CGG - chr3.3:37165317-37165336 MS.gene019178:intron 40.0%
GTTTGTGTCAGTAGCAACAA+CGG - chr3.3:37165400-37165419 MS.gene019178:CDS 40.0%
TACTGTGTCATCCATCATAC+AGG - chr3.3:37163632-37163651 MS.gene019178:intron 40.0%
TATGGTTGTGTCTCTCAAGA+AGG - chr3.3:37162331-37162350 MS.gene019178:CDS 40.0%
TATTGCCAAAATGGGCATAG+TGG - chr3.3:37165214-37165233 MS.gene019178:intron 40.0%
TCAACTCGATGACCCAAATT+AGG - chr3.3:37163240-37163259 MS.gene019178:intron 40.0%
TCCACTGTCTATTGGATACA+AGG - chr3.3:37163686-37163705 MS.gene019178:intron 40.0%
TGAAGTTGTGTCCAATTCTG+TGG - chr3.3:37162394-37162413 MS.gene019178:CDS 40.0%
TGATGAAACTTGGATGACGA+GGG + chr3.3:37164956-37164975 None:intergenic 40.0%
TGATTTACTTCTACCTGCAG+TGG - chr3.3:37162235-37162254 MS.gene019178:CDS 40.0%
TGGGAATGTACATATCGTCA+AGG + chr3.3:37164986-37165005 None:intergenic 40.0%
TGTACATATCGTCAAGGGAA+GGG + chr3.3:37164980-37164999 None:intergenic 40.0%
TGTGACAAATGAAGAGGTAG+TGG - chr3.3:37162166-37162185 MS.gene019178:CDS 40.0%
TGTGAGTGTGACAAATGAAG+AGG - chr3.3:37162160-37162179 MS.gene019178:CDS 40.0%
TGTTATTGTGTTGGGCTTGA+TGG + chr3.3:37164895-37164914 None:intergenic 40.0%
TTGATGAAACTTGGATGACG+AGG + chr3.3:37164957-37164976 None:intergenic 40.0%
TTGGCGAAGATTTATTGTGG+TGG - chr3.3:37165479-37165498 MS.gene019178:CDS 40.0%
TTTGTTCCAAAGAGGAAGCA+TGG - chr3.3:37162530-37162549 MS.gene019178:CDS 40.0%
! AAAGTGTGGTGGAATAGTAG+TGG - chr3.3:37164782-37164801 MS.gene019178:intron 40.0%
! ATCAACCACTATGCCCATTT+TGG + chr3.3:37165222-37165241 None:intergenic 40.0%
! CATTTTACTACCGTTCCTTG+TGG + chr3.3:37165192-37165211 None:intergenic 40.0%
! CCCTTTAAGGAATGGTGATT+GGG - chr3.3:37165646-37165665 MS.gene019178:CDS 40.0%
! CTTCTTAGGGCTCATTGATT+GGG - chr3.3:37165430-37165449 MS.gene019178:CDS 40.0%
! CTTGTTTCTCTAGGAATCAG+AGG + chr3.3:37162915-37162934 None:intergenic 40.0%
! GCAACGATGACACACTATTT+TGG - chr3.3:37165290-37165309 MS.gene019178:intron 40.0%
! GGATTGGCGAAGATTTATTG+TGG - chr3.3:37165476-37165495 MS.gene019178:CDS 40.0%
! GGGTTGGATAATAATGGAGT+TGG - chr3.3:37164132-37164151 MS.gene019178:intron 40.0%
! GTTAGCGGTTGTTATTGTGT+TGG + chr3.3:37164904-37164923 None:intergenic 40.0%
! TGCATCCTACCATTTTCCTT+GGG - chr3.3:37165589-37165608 MS.gene019178:CDS 40.0%
! TTGCATCCTACCATTTTCCT+TGG - chr3.3:37165588-37165607 MS.gene019178:CDS 40.0%
!! AAGACAAGGTGGATTTTGGA+TGG - chr3.3:37165552-37165571 MS.gene019178:CDS 40.0%
!! AGACAAGGTGGATTTTGGAT+GGG - chr3.3:37165553-37165572 MS.gene019178:CDS 40.0%
!! GAGTTGGTTGATAAGCCATT+AGG - chr3.3:37165353-37165372 MS.gene019178:CDS 40.0%
!!! GCATGTTGGTTTTGTGAGAT+AGG + chr3.3:37163607-37163626 None:intergenic 40.0%
!!! TCACCCCTTAGTTTTATACC+TGG + chr3.3:37162568-37162587 None:intergenic 40.0%
AACCAACTTCCACAAGTAGG+CGG + chr3.3:37165159-37165178 None:intergenic 45.0%
AAGATTTCCGGAAGACAAGG+TGG - chr3.3:37165541-37165560 MS.gene019178:CDS 45.0%
ACCCAATCAAATAGGGGTTC+AGG + chr3.3:37164173-37164192 None:intergenic 45.0%
AGCAACCAACTTCCACAAGT+AGG + chr3.3:37165162-37165181 None:intergenic 45.0%
ATTGGAGATCATAGAGTGCG+AGG - chr3.3:37165697-37165716 MS.gene019178:CDS 45.0%
ATTTGGCGGTAAATCAGTGG+AGG - chr3.3:37165331-37165350 MS.gene019178:CDS 45.0%
CATCATACAGGCTACAACCA+TGG - chr3.3:37163644-37163663 MS.gene019178:intron 45.0%
CATGGTTGTAGCCTGTATGA+TGG + chr3.3:37163646-37163665 None:intergenic 45.0%
CCAAAATCCACCTTGTCTTC+CGG + chr3.3:37165551-37165570 None:intergenic 45.0%
CGACAATTAACTGCTGCTAG+GGG - chr3.3:37164006-37164025 MS.gene019178:intron 45.0%
CTTGATGGGAATTGTTGGGT+GGG + chr3.3:37164880-37164899 None:intergenic 45.0%
GAACCCCTATTTGATTGGGT+TGG - chr3.3:37164173-37164192 MS.gene019178:intron 45.0%
GACACAGTACACTTGCATGT+TGG + chr3.3:37163621-37163640 None:intergenic 45.0%
GCAAAGATTTCCGGAAGACA+AGG - chr3.3:37165538-37165557 MS.gene019178:CDS 45.0%
GTCTCATACTATGCCTTTGC+AGG - chr3.3:37162371-37162390 MS.gene019178:CDS 45.0%
TCACCATTCCTTAAAGGGCT+TGG + chr3.3:37165644-37165663 None:intergenic 45.0%
TGACGATGGTCAAGTGACTT+GGG + chr3.3:37162799-37162818 None:intergenic 45.0%
TGGAAACGATAGTAGCGATG+AGG - chr3.3:37165256-37165275 MS.gene019178:intron 45.0%
TGGGCTTGATGGGAATTGTT+GGG + chr3.3:37164884-37164903 None:intergenic 45.0%
TTCCTTAAAGGGCTTGGCAT+TGG + chr3.3:37165638-37165657 None:intergenic 45.0%
TTGACGATGGTCAAGTGACT+TGG + chr3.3:37162800-37162819 None:intergenic 45.0%
TTGGGCTTGATGGGAATTGT+TGG + chr3.3:37164885-37164904 None:intergenic 45.0%
! AGTCTTTTTCCGCCTACTTG+TGG - chr3.3:37165147-37165166 MS.gene019178:intron 45.0%
! ATGCACCTTGTTATCCGACA+AGG - chr3.3:37163788-37163807 MS.gene019178:intron 45.0%
! ATGCCAAGCCCTTTAAGGAA+TGG - chr3.3:37165638-37165657 MS.gene019178:CDS 45.0%
! CTCGCTGCTTTAGCTAACAA+TGG - chr3.3:37165101-37165120 MS.gene019178:intron 45.0%
! GCATCCTACCATTTTCCTTG+GGG - chr3.3:37165590-37165609 MS.gene019178:CDS 45.0%
! GCCCTTTAAGGAATGGTGAT+TGG - chr3.3:37165645-37165664 MS.gene019178:CDS 45.0%
! GCTAAGTAACGGCTTTGTAG+CGG + chr3.3:37165036-37165055 None:intergenic 45.0%
! GTTGATGGAAGAAGGAGACT+TGG - chr3.3:37165236-37165255 MS.gene019178:intron 45.0%
! TGCACCTTGTTATCCGACAA+GGG - chr3.3:37163789-37163808 MS.gene019178:intron 45.0%
! TGTGTCTCTCAAGAAGGCAT+TGG - chr3.3:37162337-37162356 MS.gene019178:CDS 45.0%
! TTAACTGCTGCTAGGGGTAT+TGG - chr3.3:37164012-37164031 MS.gene019178:intron 45.0%
!! AAGTTCTAGGGTGGAAGTTG+TGG + chr3.3:37162867-37162886 None:intergenic 45.0%
!! AGGGAAGGGTTGATGAAACT+TGG + chr3.3:37164966-37164985 None:intergenic 45.0%
!! CCACTGATTTACCGCCAAAT+GGG + chr3.3:37165331-37165350 None:intergenic 45.0%
!! GACAAGGTGGATTTTGGATG+GGG - chr3.3:37165554-37165573 MS.gene019178:CDS 45.0%
!! GCATAGTGGTTGATGGAAGA+AGG - chr3.3:37165228-37165247 MS.gene019178:intron 45.0%
!! GGTTGATAAGCCATTAGGGT+GGG - chr3.3:37165358-37165377 MS.gene019178:CDS 45.0%
!! TCCACTGATTTACCGCCAAA+TGG + chr3.3:37165332-37165351 None:intergenic 45.0%
!! TCTCAAGAAGGCATTGGCTA+AGG - chr3.3:37162343-37162362 MS.gene019178:CDS 45.0%
!! TGGTTGATAAGCCATTAGGG+TGG - chr3.3:37165357-37165376 MS.gene019178:CDS 45.0%
!!! GAAGGCATTGGCTAAGGTTT+TGG - chr3.3:37162349-37162368 MS.gene019178:CDS 45.0%
!!! GGTTCAGGTTGGACCAATTT+TGG + chr3.3:37164158-37164177 None:intergenic 45.0%
!!! AAATATTATAATTTTATTTG+AGG - chr3.3:37163425-37163444 MS.gene019178:intron 5.0%
AACACACCAACATCCACTGC+AGG + chr3.3:37162251-37162270 None:intergenic 50.0%
AAGGGGGTGGTAGATGCAAA+AGG - chr3.3:37163807-37163826 MS.gene019178:intron 50.0%
ACAAAAGCCGGCCCTTCATT+GGG + chr3.3:37165506-37165525 None:intergenic 50.0%
ACCACCCCAAGGAAAATGGT+AGG + chr3.3:37165597-37165616 None:intergenic 50.0%
ACGATAGTAGCGATGAGGAC+CGG - chr3.3:37165261-37165280 MS.gene019178:intron 50.0%
AGGAGGAGGGAGTTTGGAAA+TGG + chr3.3:37165006-37165025 None:intergenic 50.0%
AGGAGTCGTGTTCAGGAGTT+GGG - chr3.3:37163450-37163469 MS.gene019178:intron 50.0%
ATTGTGGTGGCCCCAATGAA+GGG - chr3.3:37165492-37165511 MS.gene019178:CDS 50.0%
CCCATTTGGCGGTAAATCAG+TGG - chr3.3:37165328-37165347 MS.gene019178:intron 50.0%
CGGTAAATCAGTGGAGGAGT+TGG - chr3.3:37165337-37165356 MS.gene019178:CDS 50.0%
CTCAAGCTTCGAAGGCTTAG+TGG + chr3.3:37165129-37165148 None:intergenic 50.0%
CTCAGGTTCACCCACAGAAT+TGG + chr3.3:37162408-37162427 None:intergenic 50.0%
CTTCACCACCCCAAGGAAAA+TGG + chr3.3:37165601-37165620 None:intergenic 50.0%
CTTCTACCTGCAGTGGATGT+TGG - chr3.3:37162242-37162261 MS.gene019178:CDS 50.0%
GCTAATGCAGCCACAAGGAA+CGG - chr3.3:37165179-37165198 MS.gene019178:intron 50.0%
GCTTGATGGGAATTGTTGGG+TGG + chr3.3:37164881-37164900 None:intergenic 50.0%
GGACACAACTTCACCTGCAA+AGG + chr3.3:37162387-37162406 None:intergenic 50.0%
GGAGGAGGGAGTTTGGAAAT+GGG + chr3.3:37165005-37165024 None:intergenic 50.0%
GTCTTCGGGGCAAAGATTTC+CGG - chr3.3:37165529-37165548 MS.gene019178:CDS 50.0%
GTGTCATCGTTGCTTCTCTC+CGG + chr3.3:37165283-37165302 None:intergenic 50.0%
TATTGTGGTGGCCCCAATGA+AGG - chr3.3:37165491-37165510 MS.gene019178:CDS 50.0%
TCATCAACCACCCACCCTAA+TGG + chr3.3:37165371-37165390 None:intergenic 50.0%
TGATAAGCCATTAGGGTGGG+TGG - chr3.3:37165361-37165380 MS.gene019178:CDS 50.0%
TGCCAATGCCAAGCCCTTTA+AGG - chr3.3:37165633-37165652 MS.gene019178:CDS 50.0%
TGGTTGCTAATGCAGCCACA+AGG - chr3.3:37165174-37165193 MS.gene019178:intron 50.0%
TGTGTCAGTAGCAACAACGG+AGG - chr3.3:37165403-37165422 MS.gene019178:CDS 50.0%
TTCCAAACTCCCTCCTCCTT+CGG - chr3.3:37165007-37165026 MS.gene019178:intron 50.0%
TTCCGCCTACTTGTGGAAGT+TGG - chr3.3:37165154-37165173 MS.gene019178:intron 50.0%
! AAGTAACGGCTTTGTAGCGG+TGG + chr3.3:37165033-37165052 None:intergenic 50.0%
! ATTAGGGTGGGTGGTTGATG+AGG - chr3.3:37165370-37165389 MS.gene019178:CDS 50.0%
! CACCTTGTTATCCGACAAGG+GGG - chr3.3:37163791-37163810 MS.gene019178:intron 50.0%
! CTTAGGGCTCATTGATTGGG+TGG - chr3.3:37165433-37165452 MS.gene019178:CDS 50.0%
! GCACCTTGTTATCCGACAAG+GGG - chr3.3:37163790-37163809 MS.gene019178:intron 50.0%
! TCCTACCATTTTCCTTGGGG+TGG - chr3.3:37165593-37165612 MS.gene019178:CDS 50.0%
!! AATTGTTGGGTGGGCTACCT+CGG + chr3.3:37164871-37164890 None:intergenic 50.0%
!! ACAACGGAGGAGCACTTCTT+AGG - chr3.3:37165416-37165435 MS.gene019178:CDS 50.0%
!! ACAGGGGTATTCGTTGACGA+TGG + chr3.3:37162813-37162832 None:intergenic 50.0%
!! CAACGGAGGAGCACTTCTTA+GGG - chr3.3:37165417-37165436 MS.gene019178:CDS 50.0%
!! CAAGCGTTGGAGCTTCTCTA+CGG + chr3.3:37165075-37165094 None:intergenic 50.0%
!! CCGGAAGACAAGGTGGATTT+TGG - chr3.3:37165548-37165567 MS.gene019178:CDS 50.0%
AACTTGGATGACGAGGGCTC+AGG + chr3.3:37164950-37164969 None:intergenic 55.0%
AGCACGCCATGCTTCCTCTT+TGG + chr3.3:37162539-37162558 None:intergenic 55.0%
AGGAGGGAGCGACGAAAACA+TGG + chr3.3:37164930-37164949 None:intergenic 55.0%
ATAGGCTACAACGGCGTCGT+TGG + chr3.3:37163589-37163608 None:intergenic 55.0%
ATCTTCGCCAATCCAGGGAC+AGG + chr3.3:37165470-37165489 None:intergenic 55.0%
CAAAAGCCGGCCCTTCATTG+GGG + chr3.3:37165505-37165524 None:intergenic 55.0%
CACAAAAGCCGGCCCTTCAT+TGG + chr3.3:37165507-37165526 None:intergenic 55.0%
CACCCCCTTGTCGGATAACA+AGG + chr3.3:37163796-37163815 None:intergenic 55.0%
CAGCGAGTGATTGCAAGCGT+TGG + chr3.3:37165088-37165107 None:intergenic 55.0%
CAGGAGTCGTGTTCAGGAGT+TGG - chr3.3:37163449-37163468 MS.gene019178:intron 55.0%
CCCGAAGACACCACAAAAGC+CGG + chr3.3:37165518-37165537 None:intergenic 55.0%
CTACCTCGGCCCATGATACT+AGG + chr3.3:37164857-37164876 None:intergenic 55.0%
GTTCCTAGTATCATGGGCCG+AGG - chr3.3:37164851-37164870 MS.gene019178:intron 55.0%
TAGGCTACAACGGCGTCGTT+GGG + chr3.3:37163588-37163607 None:intergenic 55.0%
TAGGGTGGAAGTTGTGGTGC+TGG + chr3.3:37162861-37162880 None:intergenic 55.0%
TGAGGTCAGGAGTCGTGTTC+AGG - chr3.3:37163443-37163462 MS.gene019178:intron 55.0%
TGCATCTACCACCCCCTTGT+CGG + chr3.3:37163805-37163824 None:intergenic 55.0%
TTTCCTTGGGGTGGTGAAGC+TGG - chr3.3:37165602-37165621 MS.gene019178:CDS 55.0%
! CCGGCTTTTGTGGTGTCTTC+GGG - chr3.3:37165515-37165534 MS.gene019178:CDS 55.0%
! CGATGGTCAAGTGACTTGGG+TGG + chr3.3:37162796-37162815 None:intergenic 55.0%
! CGGCTTTTGTGGTGTCTTCG+GGG - chr3.3:37165516-37165535 MS.gene019178:CDS 55.0%
! CTTGTTATCCGACAAGGGGG+TGG - chr3.3:37163794-37163813 MS.gene019178:intron 55.0%
!! CAATGAAGGGCCGGCTTTTG+TGG - chr3.3:37165505-37165524 MS.gene019178:CDS 55.0%
!! GCCGGCTTTTGTGGTGTCTT+CGG - chr3.3:37165514-37165533 MS.gene019178:CDS 55.0%
ACACCGACCTGTCCCTGGAT+TGG - chr3.3:37165460-37165479 MS.gene019178:CDS 60.0%
TAGCCAGCTTCACCACCCCA+AGG + chr3.3:37165608-37165627 None:intergenic 60.0%
TCGCCAATCCAGGGACAGGT+CGG + chr3.3:37165466-37165485 None:intergenic 60.0%
TGGATGACGAGGGCTCAGGA+GGG + chr3.3:37164946-37164965 None:intergenic 60.0%
TTGGATGACGAGGGCTCAGG+AGG + chr3.3:37164947-37164966 None:intergenic 60.0%
TTTGTAGCGGTGGCCGAAGG+AGG + chr3.3:37165023-37165042 None:intergenic 60.0%
!! TGCTGGCTCACGACAGAGCT+TGG + chr3.3:37162844-37162863 None:intergenic 60.0%
GAAGCACACCGACCTGTCCC+TGG - chr3.3:37165455-37165474 MS.gene019178:CDS 65.0%
GGCCGAAGGAGGAGGGAGTT+TGG + chr3.3:37165012-37165031 None:intergenic 65.0%
TAGCGGTGGCCGAAGGAGGA+GGG + chr3.3:37165019-37165038 None:intergenic 65.0%
TGGTGGCCCCAATGAAGGGC+CGG - chr3.3:37165496-37165515 MS.gene019178:CDS 65.0%
! GGCTTTGTAGCGGTGGCCGA+AGG + chr3.3:37165026-37165045 None:intergenic 65.0%
GCATGGCGTGCTCGCAGTCC+AGG - chr3.3:37162547-37162566 MS.gene019178:CDS 70.0%
GTAGCGGTGGCCGAAGGAGG+AGG + chr3.3:37165020-37165039 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 37162131 37165766 37162131 ID=MS.gene019178
chr3.3 mRNA 37162131 37165766 37162131 ID=MS.gene019178.t1;Parent=MS.gene019178
chr3.3 exon 37165329 37165766 37165329 ID=MS.gene019178.t1.exon1;Parent=MS.gene019178.t1
chr3.3 CDS 37165329 37165766 37165329 ID=cds.MS.gene019178.t1;Parent=MS.gene019178.t1
chr3.3 exon 37162131 37163126 37162131 ID=MS.gene019178.t1.exon2;Parent=MS.gene019178.t1
chr3.3 CDS 37162131 37163126 37162131 ID=cds.MS.gene019178.t1;Parent=MS.gene019178.t1
Gene Sequence

>MS.gene019178

ATGGATAATGGAAATGGAGATAATTTCATTGTGAGTGTGACAAATGAAGAGGTAGTGGCAGCAGTGTTACCAATACAAGAACATTGGTTGCCACTCTCTAACCTTGATTTACTTCTACCTGCAGTGGATGTTGGTGTGTTCTTTTGTTACAAGAATCCCATGATATTGTCCACTTCAACAACCTTATTAACCTTTGAATCTATGGTTGTGTCTCTCAAGAAGGCATTGGCTAAGGTTTTGGTCTCATACTATGCCTTTGCAGGTGAAGTTGTGTCCAATTCTGTGGGTGAACCTGAGCTTCTTTGTAATAACCGTGGTGTTGATTTTGTTGAAGCTGTTGCTGATGTTGAGCTTCAATGCCTCAATTTTTATAACCCTGATGAATCTGTTGAAGGAAAGTTTGTTCCAAAGAGGAAGCATGGCGTGCTCGCAGTCCAGGTATAAAACTAAGGGGTGACAAAATGGATTGATTGAATATATTTTATTCGGACAGTAATAGTTTTTTTTTAATAAAGCAAAAACAAAATATATAAATGATTTTGAATAACTGTAATAGATGGATTTAAACAATTCATTAATTCATTTTAAATTTTAAAAAGCTCAAAAATAAAATCAAATTCATTCATTAACCATTAAAAAATTTCAATGTGGATTCTGATTCACCACCCAAGTCACTTGACCATCGTCAACGAATACCCCTGTAAAATTTTCCAAGCTCTGTCGTGAGCCAGCACCACAACTTCCACCCTAGAACTTTTATTACTTTTTTGTTGTGTGTTAACCTCTGATTCCTAGAGAAACAAGACTTTTGTAATCGAGAGTTCAATTGAAAGGTCAGTAAAACTTGGCAAAAAATTATTCTATCAGGAATCAAATGTATAATTATTTTAGTAAAATATGATTAGTATCAACTAATGATAAAAAATTACATGTATTATTTTTTAATATTACAAAAAAAGTATTATTTTTTAAATCCTTTATCTGCCTATATAAAAAAAATCCTTTATCTAATATTCTGAATAAAAAACTATTATTCAATGTATTTTATTGATTAATTTTTTTAAACGAATGTTATAATTTTATTTGAGGTCAGGAGTTGGGAGACACATTCAACTCGATGACCCAAATTAGGTTATAATTATTTTAGTAAAATATGATTAATATCAACTAATAATAAAACATTATATGTATTCTTTTGTAAATCATTTATCTGCTTATAAAAATATCCTTTATCTAATATTTTGAATAAAAAAAACTATTATTTAATGTATTTTATTGATTAATTTTTTTTAACAAATATTATAATTTTATTTGAGGTCAGGAGTCGTGTTCAGGAGTTGGGTGACACATTCAACCCGATGACTTAAAAGTATACTAATATCTTCAACAGTAGTTAACTGTTGCTACCAATGACAGTTAATTGCCACTACGTTACAAAAATTGAAAAAAAAACACCCAACGACGCCGTTGTAGCCTATCTCACAAAACCAACATGCAAGTGTACTGTGTCATCCATCATACAGGCTACAACCATGGGTTATGTGTAAGAGATTATTCCACTGTCTATTGGATACAAGGTACGTAAATCCATTTAGATGACCCACCTTAAGCTTATATAAGAATGATGGAATAGTCACTACTTGAATAGTGGTCTACAATGCACCTTGTTATCCGACAAGGGGGTGGTAGATGCAAAAGGCAAAATGGATGGTATTGGACATTTGAAATGGATATGGACTGTGATATAGGCCATTGATAAGCACTCAAATGCACATTTATCATGTTAGTTTTGTGAGATAGGATACAAAGGTTCTTAAATACCCTTTTTTTTTTTTCTTTTTTCTTTCTTTCTTTCTTTCAATTTTTGCAACATAACGACAATTAACTGCTGCTAGGGGTATTGGTTTTTTTTTGAGGTGCCAATAAGCAAAAATCAATGTGAAAAGAGAATTTTCTTAAAAGATGCGATTTATTTTTACTAGACGTCATTGAAATTTGATTGGGTTGGATAATAATGGAGTTGGCCAAAATTGGTCCAACCTGAACCCCTATTTGATTGGGTTGGATAATAATGGAGTATTTTCTCTCTCTACTTTATGTCTTCATTTTTTTTTGTTAAGAAGTCTAGTCGTTAGACTCACACATTTTAAGTGTGGAAAATTCGAGGTTCGAATCTAAACTCATGCATATCAATGTTCATAGAGCTTACCATCCGAGTTACATTTAAACGACACATCTTCATTTCTTTAGTTTTAAAGAGTTTTAAAGTGTATATTTAATAATTAGACACGTCAAATGTCATATTATTGTATTTGTATTCTCATACATTTGGTAATGAATAAATGAAGAGGATAAAAAATAAAAGGATCTAGACTCATATTTAAGATGATCCAATTGATTATAAGATGAATGGATGAATTGAATTCGATCCATTATTTCTTTTTGCTCTAATTAAGAATGGATTGGATACGGAAAAATGATCAATTTTGCCACCCTTAAAGCCAATTTTATGGTAGCCATATTATGTCAGATTATTTTCGAAATTTTGTTTTAAAATCTTGCAAAGTTAAACGATTTTGTGTAATATTGAAGTATAGTAACTTAAGTTTAGTGTTTTAATTGACTTTATTTTTTATGCAGGCAACTTCATTAAAGTGTGGTGGAATAGTAGTGGCATGCACATTTGATCATCGCATAGCAGATGCTTATTCAACAAACATGTTCCTAGTATCATGGGCCGAGGTAGCCCACCCAACAATTCCCATCAAGCCCAACACAATAACAACCGCTAACAAACCATGTTTTCGTCGCTCCCTCCTGAGCCCTCGTCATCCAAGTTTCATCAACCCTTCCCTTGACGATATGTACATTCCCATTTCCAAACTCCCTCCTCCTTCGGCCACCGCTACAAAGCCGTTACTTAGCCGAATATACTACGTCACCGTAGAGAAGCTCCAACGCTTGCAATCACTCGCTGCTTTAGCTAACAATGGCACCACTAAGCCTTCGAAGCTTGAGTCTTTTTCCGCCTACTTGTGGAAGTTGGTTGCTAATGCAGCCACAAGGAACGGTAGTAAAATGGTTATTGCCAAAATGGGCATAGTGGTTGATGGAAGAAGGAGACTTGGAAACGATAGTAGCGATGAGGACCGGAGAGAAGCAACGATGACACACTATTTTGGTAATGTTCTTTCTATCCCATTTGGCGGTAAATCAGTGGAGGAGTTGGTTGATAAGCCATTAGGGTGGGTGGTTGATGAGGTTCGCGAGTTTGTGTCAGTAGCAACAACGGAGGAGCACTTCTTAGGGCTCATTGATTGGGTGGAAGCACACCGACCTGTCCCTGGATTGGCGAAGATTTATTGTGGTGGCCCCAATGAAGGGCCGGCTTTTGTGGTGTCTTCGGGGCAAAGATTTCCGGAAGACAAGGTGGATTTTGGATGGGGAAAAGTTGTTTTTGCATCCTACCATTTTCCTTGGGGTGGTGAAGCTGGCTATGTGATGCCAATGCCAAGCCCTTTAAGGAATGGTGATTGGGTTGTTTACATGCATCTTTTGAAAGAGCAATTGGAGATCATAGAGTGCGAGGCAGATCATGTCTTTATGCCTTTGACTTGGGATTATCTCAATCAATGA
Protein sequence

>MS.gene019178.t1

MDNGNGDNFIVSVTNEEVVAAVLPIQEHWLPLSNLDLLLPAVDVGVFFCYKNPMILSTSTTLLTFESMVVSLKKALAKVLVSYYAFAGEVVSNSVGEPELLCNNRGVDFVEAVADVELQCLNFYNPDESVEGKFVPKRKHGVLAVQATSLKCGGIVVACTFDHRIADAYSTNMFLVSWAEVAHPTIPIKPNTITTANKPCFRRSLLSPRHPSFINPSLDDMYIPISKLPPPSATATKPLLSRIYYVTVEKLQRLQSLAALANNGTTKPSKLESFSAYLWKLVANAATRNGSKMVIAKMGIVVDGRRRLGNDSSDEDRREATMTHYFGNVLSIPFGGKSVEELVDKPLGWVVDEVREFVSVATTEEHFLGLIDWVEAHRPVPGLAKIYCGGPNEGPAFVVSSGQRFPEDKVDFGWGKVVFASYHFPWGGEAGYVMPMPSPLRNGDWVVYMHLLKEQLEIIECEADHVFMPLTWDYLNQ