AlfalfaGEDB | MS.gene038271

M. sativa cultivar XinJiangDaYe / MS.gene038271

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038271.t1	XP_003617368.1	93.7	473	22	3	1	467	1	471	8.40E-264	919.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038271.t1	Q9FI78	24.8	456	297	15	10	450	3	427	2.1e-29	131.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038271.t1	G7K3A2	93.7	473	22	3	1	467	1	471	6.0e-264	919.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene38701	MS.gene038271	PPI
MS.gene038271	MS.gene82145	PPI
MS.gene046246	MS.gene038271	PPI
MS.gene38700	MS.gene038271	PPI
MS.gene38365	MS.gene038271	PPI
MS.gene012965	MS.gene038271	PPI
MS.gene95242	MS.gene038271	PPI
MS.gene019178	MS.gene038271	PPI
MS.gene017054	MS.gene038271	PPI
MS.gene017058	MS.gene038271	PPI
MS.gene038271	MS.gene47719	PPI
MS.gene000507	MS.gene038271	PPI
MS.gene038271	MS.gene000507	PPI
MS.gene028134	MS.gene038271	PPI
MS.gene038271	MS.gene38702	PPI
MS.gene69496	MS.gene038271	PPI
MS.gene22199	MS.gene038271	PPI
MS.gene32936	MS.gene038271	PPI
MS.gene039597	MS.gene038271	PPI
MS.gene72785	MS.gene038271	PPI
MS.gene48027	MS.gene038271	PPI
MS.gene83461	MS.gene038271	PPI
MS.gene038271	MS.gene012961	PPI
MS.gene32938	MS.gene038271	PPI
MS.gene48021	MS.gene038271	PPI
MS.gene038271	MS.gene32933	PPI
MS.gene38362	MS.gene038271	PPI
MS.gene038271	MS.gene038274	PPI
MS.gene017053	MS.gene038271	PPI
MS.gene47716	MS.gene038271	PPI
MS.gene38703	MS.gene038271	PPI
MS.gene069321	MS.gene038271	PPI
MS.gene038271	MS.gene69496	PPI
MS.gene038271	MS.gene38359	PPI
MS.gene38363	MS.gene038271	PPI
MS.gene38706	MS.gene038271	PPI
MS.gene32934	MS.gene038271	PPI
MS.gene038271	MS.gene38699	PPI
MS.gene48023	MS.gene038271	PPI
MS.gene012960	MS.gene038271	PPI
MS.gene38704	MS.gene038271	PPI
MS.gene038271	MS.gene012959	PPI
MS.gene012957	MS.gene038271	PPI
MS.gene38361	MS.gene038271	PPI
MS.gene038274	MS.gene038271	PPI
MS.gene47721	MS.gene038271	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038271.t1	MTR_5g090830	95.137	473	15	3	1	467	1	471	0.0	929
MS.gene038271.t1	MTR_5g090810	93.023	473	25	3	1	467	1	471	0.0	916
MS.gene038271.t1	MTR_5g090880	79.704	473	90	2	1	467	1	473	0.0	772
MS.gene038271.t1	MTR_3g450710	65.690	478	141	8	1	465	1	468	0.0	615
MS.gene038271.t1	MTR_3g450690	63.125	480	154	7	1	465	1	472	0.0	600
MS.gene038271.t1	MTR_5g026320	34.475	438	254	13	7	435	15	428	6.38e-69	227
MS.gene038271.t1	MTR_8g075610	25.629	437	269	16	32	450	16	414	2.59e-32	127
MS.gene038271.t1	MTR_8g096530	28.571	441	255	19	31	453	28	426	3.76e-32	127
MS.gene038271.t1	MTR_2g064495	25.000	460	290	17	10	450	3	426	2.84e-31	125
MS.gene038271.t1	MTR_2g105330	25.000	460	290	17	10	450	3	426	1.11e-30	123
MS.gene038271.t1	MTR_4g007540	24.396	414	271	12	10	409	3	388	3.23e-30	122
MS.gene038271.t1	MTR_4g097090	26.022	465	290	15	23	460	19	456	1.24e-28	118
MS.gene038271.t1	MTR_3g049150	26.424	439	265	18	31	441	23	431	8.08e-28	115
MS.gene038271.t1	MTR_3g098980	26.598	391	238	13	31	408	35	389	9.90e-28	115
MS.gene038271.t1	MTR_7g114470	26.415	424	253	15	8	410	9	394	2.48e-27	114
MS.gene038271.t1	MTR_7g114560	24.672	458	283	13	8	442	8	426	2.68e-27	114
MS.gene038271.t1	MTR_7g027175	26.718	393	238	14	31	409	37	393	7.91e-27	112
MS.gene038271.t1	MTR_1g040215	26.434	401	229	14	63	431	61	427	1.01e-26	112
MS.gene038271.t1	MTR_7g114550	24.891	458	282	14	8	442	8	426	1.17e-26	112
MS.gene038271.t1	MTR_4g127650	23.932	468	295	14	23	460	20	456	1.59e-26	112
MS.gene038271.t1	MTR_5g076700	25.055	455	286	16	8	442	8	427	1.32e-25	109
MS.gene038271.t1	MTR_7g086000	28.000	375	211	16	66	407	60	408	3.35e-25	108
MS.gene038271.t1	MTR_1g040175	25.310	403	231	13	63	431	61	427	8.11e-25	107
MS.gene038271.t1	MTR_7g114490	23.881	469	295	14	8	453	8	437	1.15e-23	103
MS.gene038271.t1	MTR_7g114520	25.546	458	279	18	8	441	9	428	6.34e-23	101
MS.gene038271.t1	MTR_3g026950	23.649	444	293	11	31	450	23	444	2.57e-21	96.7
MS.gene038271.t1	MTR_1g053315	24.079	407	250	14	29	410	40	412	4.13e-21	95.9
MS.gene038271.t1	MTR_7g085930	27.595	395	229	19	66	424	59	432	1.22e-19	91.7
MS.gene038271.t1	MTR_7g085990	25.654	382	223	17	66	409	55	413	2.38e-19	90.9
MS.gene038271.t1	MTR_1g040195	24.796	367	210	12	96	431	71	402	1.68e-18	87.8
MS.gene038271.t1	MTR_4g055170	24.176	455	292	18	17	446	19	445	1.99e-17	84.7
MS.gene038271.t1	MTR_2g015430	23.940	401	255	16	24	408	18	384	6.40e-17	83.2
MS.gene038271.t1	MTR_3g101900	22.683	410	258	10	27	408	19	397	6.81e-17	83.2
MS.gene038271.t1	MTR_7g084940	24.823	423	257	14	7	409	13	394	7.39e-17	83.2
MS.gene038271.t1	MTR_7g056087	24.183	459	294	18	10	447	4	429	1.98e-16	81.6
MS.gene038271.t1	MTR_3g086020	24.186	430	252	18	7	421	2	372	2.30e-16	81.3
MS.gene038271.t1	MTR_7g084910	24.783	460	254	19	7	429	14	418	3.65e-16	80.9
MS.gene038271.t1	MTR_7g114510	25.091	275	174	9	137	410	9	252	2.00e-15	77.4
MS.gene038271.t1	MTR_4g094842	24.074	378	221	13	61	408	57	398	2.95e-14	75.1
MS.gene038271.t1	MTR_8g079980	22.973	444	276	16	12	434	5	403	3.32e-14	74.7
MS.gene038271.t1	MTR_3g101960	23.511	319	196	13	49	343	48	342	3.87e-14	74.7
MS.gene038271.t1	MTR_3g465990	23.894	339	212	11	49	365	50	364	8.79e-14	73.6
MS.gene038271.t1	MTR_3g088420	24.365	394	237	15	36	408	32	385	1.19e-13	73.2
MS.gene038271.t1	MTR_3g101930	22.251	382	246	11	49	406	49	403	2.68e-13	72.0
MS.gene038271.t1	MTR_8g041770	24.020	408	250	14	23	408	22	391	1.28e-12	70.1
MS.gene038271.t1	MTR_4g022670	23.822	382	238	13	49	408	43	393	6.50e-12	67.8
MS.gene038271.t1	MTR_3g465980	23.894	339	212	11	49	365	50	364	6.73e-12	67.8
MS.gene038271.t1	MTR_0042s0190	23.552	259	162	9	154	410	1	225	9.66e-12	66.2
MS.gene038271.t1	MTR_3g026310	25.253	396	246	16	36	408	32	400	9.98e-12	67.0
MS.gene038271.t1	MTR_4g022720	24.000	375	229	12	59	408	44	387	1.20e-11	67.0
MS.gene038271.t1	MTR_4g022290	22.906	406	231	15	36	408	38	394	2.47e-11	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038271.t1	AT1G78990	58.190	464	167	9	8	465	11	453	8.61e-179	508
MS.gene038271.t1	AT1G32910	59.534	472	153	13	8	465	11	458	1.45e-178	508
MS.gene038271.t1	AT5G16410	51.899	474	184	14	8	465	34	479	3.38e-158	457
MS.gene038271.t1	AT1G31490	36.795	443	231	14	8	435	4	412	1.11e-75	244
MS.gene038271.t1	AT5G48930	25.383	457	293	16	10	450	3	427	1.64e-35	137
MS.gene038271.t1	AT5G41040	25.570	395	242	13	31	409	38	396	1.31e-28	117
MS.gene038271.t1	AT5G41040	25.570	395	242	13	31	409	54	412	1.75e-28	117
MS.gene038271.t1	AT2G19070	24.301	465	305	15	18	460	12	451	2.20e-27	114
MS.gene038271.t1	AT5G57840	26.338	467	272	18	10	446	3	427	4.43e-27	113
MS.gene038271.t1	AT5G17540	25.918	463	261	17	4	434	3	415	5.27e-27	113
MS.gene038271.t1	AT3G03480	25.541	462	264	18	8	431	17	436	1.81e-25	108
MS.gene038271.t1	AT3G48720	26.005	423	259	12	3	409	2	386	2.79e-25	108
MS.gene038271.t1	AT5G63560	25.000	420	253	14	8	408	5	381	2.66e-22	99.4
MS.gene038271.t1	AT1G27620	22.693	401	258	14	66	450	58	422	1.98e-19	90.9
MS.gene038271.t1	AT1G03390	24.485	437	271	18	2	410	4	409	3.11e-19	90.5
MS.gene038271.t1	AT3G50270	26.330	376	217	14	60	408	56	398	4.08e-19	90.1
MS.gene038271.t1	AT3G50280	24.259	371	227	14	61	408	54	393	1.38e-18	88.2
MS.gene038271.t1	AT1G28680	26.554	354	215	16	69	408	65	387	7.02e-18	86.3
MS.gene038271.t1	AT1G24430	23.294	425	247	16	10	408	5	376	4.56e-17	83.6
MS.gene038271.t1	AT2G39980	24.088	411	237	12	45	408	47	429	8.15e-17	83.2
MS.gene038271.t1	AT2G40230	22.705	414	258	13	59	450	52	425	1.03e-16	82.4
MS.gene038271.t1	AT3G30280	25.400	437	259	14	10	426	6	395	2.67e-16	81.3
MS.gene038271.t1	AT1G28680	26.367	311	194	13	69	365	65	354	8.89e-16	79.3
MS.gene038271.t1	AT5G47950	23.312	459	279	16	10	450	6	409	2.00e-14	75.5
MS.gene038271.t1	AT5G01210	24.211	380	202	13	79	408	75	418	3.19e-13	72.0
MS.gene038271.t1	AT4G15390	24.408	422	258	17	10	409	8	390	4.03e-13	71.6
MS.gene038271.t1	AT4G15390	24.408	422	258	17	10	409	8	390	4.03e-13	71.6
MS.gene038271.t1	AT3G50300	24.457	368	226	15	63	408	56	393	4.36e-13	71.6
MS.gene038271.t1	AT5G07080	23.306	369	234	11	60	408	64	403	6.49e-13	70.9
MS.gene038271.t1	AT1G24420	23.864	440	251	18	7	414	2	389	9.60e-13	70.5
MS.gene038271.t1	AT1G24420	23.864	440	251	18	7	414	2	389	9.60e-13	70.5
MS.gene038271.t1	AT2G25150	23.626	364	233	12	63	408	64	400	4.07e-12	68.6
MS.gene038271.t1	AT4G15400	24.374	439	265	16	7	424	2	394	4.27e-12	68.2
MS.gene038271.t1	AT5G07850	23.288	365	226	13	66	408	70	402	6.84e-12	67.8
MS.gene038271.t1	AT5G47980	22.535	426	261	13	7	407	2	383	1.14e-11	67.0
MS.gene038271.t1	AT5G67160	23.788	433	253	19	59	467	49	428	1.75e-11	66.2
MS.gene038271.t1	AT3G50290	24.060	266	172	10	143	405	31	269	8.40e-11	63.2

Find 129 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGATTATGAATACATATTT+TGG	0.094045	5.4:-71624398	MS.gene038271:CDS
CTCTGCTTTCTTGTGGAAAA+TGG	0.129020	5.4:-71624522	MS.gene038271:CDS
CCAGAGTCGAAGGTTGATTT+TGG	0.189640	5.4:-71624134	MS.gene038271:CDS
TAGTATATGCGACTTATTAT+TGG	0.243557	5.4:+71624632	None:intergenic
AGTACACCATGTTTCTTCTT+TGG	0.264818	5.4:+71625225	None:intergenic
TTAATATCAGAGTCAGATTC+AGG	0.280399	5.4:+71624662	None:intergenic
GTTTCTTATTATGCCTTTGC+TGG	0.282844	5.4:-71625390	MS.gene038271:CDS
ATTTCTTGGGTCTAATTGAT+TGG	0.308031	5.4:-71624259	MS.gene038271:CDS
GGATGGAAGGTGGGCGTCGT+GGG	0.311586	5.4:+71624741	None:intergenic
AAGAAACATGTTTGCAGAAT+AGG	0.336073	5.4:+71624850	None:intergenic
TAAGGGACACTTGCACTTCA+TGG	0.343370	5.4:-71623994	MS.gene038271:CDS
CAAACATGTTTCTTGTATCA+TGG	0.346322	5.4:-71624841	MS.gene038271:CDS
AGTGACAGAGGAGCATTTCT+TGG	0.350299	5.4:-71624273	MS.gene038271:CDS
AGCAGCCACTATTTCTTTGT+TGG	0.356679	5.4:+71625575	None:intergenic
TGGATGGAAGGTGGGCGTCG+TGG	0.357861	5.4:+71624740	None:intergenic
TGCCAATGCCAAGCCCTAAA+AGG	0.358606	5.4:-71624049	MS.gene038271:CDS
AAGAACACACCAACATCAAC+TGG	0.358958	5.4:+71625495	None:intergenic
TTTCCTTGGGGAGGTAGTGC+TGG	0.358995	5.4:-71624080	MS.gene038271:CDS
AGTATATGCGACTTATTATT+GGG	0.359817	5.4:+71624633	None:intergenic
AAGAGATTGGTCTAAAGAAA+TGG	0.367262	5.4:+71623958	None:intergenic
TCAAACACCCCACAATGTTA+TGG	0.372360	5.4:+71625436	None:intergenic
TCAGAGTCAGATTCAGGTTC+AGG	0.387820	5.4:+71624668	None:intergenic
TGGTTGATAATCCGCTATGC+TGG	0.388210	5.4:-71624331	MS.gene038271:CDS
CGAAAACAAGGTTGCATTGT+TGG	0.392923	5.4:+71624779	None:intergenic
CAATAAATCCTTGCCAATCC+TGG	0.393745	5.4:+71624215	None:intergenic
GATCAAAGACTTGTTATCAT+TGG	0.395387	5.4:+71624802	None:intergenic
TTTCTTGGGTCTAATTGATT+GGG	0.397686	5.4:-71624258	MS.gene038271:CDS
ATTTCTTTAGACCAATCTCT+TGG	0.398784	5.4:-71623956	MS.gene038271:CDS
CCAAAATCAACCTTCGACTC+TGG	0.403209	5.4:+71624134	None:intergenic
ATCAAAGACTTGTTATCATT+GGG	0.403427	5.4:+71624803	None:intergenic
GAAGAGCATCGACCAGTTCC+AGG	0.408207	5.4:-71624233	MS.gene038271:CDS
TTCCCTTCAACAGATTCATC+TGG	0.408828	5.4:+71625255	None:intergenic
ATTATCCATTGAAGCGGCTT+CGG	0.412620	5.4:+71624496	None:intergenic
AGAGATTGGTCTAAAGAAAT+GGG	0.420021	5.4:+71623959	None:intergenic
ATTAGGTCAAGGTTTGATAG+TGG	0.421854	5.4:+71625525	None:intergenic
CTAGTCTCCAAGATGTGTAT+TGG	0.425893	5.4:-71625624	None:intergenic
CCAACATTTGTGGTATCTTC+AGG	0.429306	5.4:-71624167	MS.gene038271:CDS
GAAAACAAGGTTGCATTGTT+GGG	0.429963	5.4:+71624780	None:intergenic
ATCCTTGCCAATCCTGGAAC+TGG	0.440383	5.4:+71624221	None:intergenic
ATCATTGGGCCGAGCTATCT+CGG	0.444473	5.4:+71624817	None:intergenic
ATGAAGTGCAAGTGTCCCTT+AGG	0.455932	5.4:+71623996	None:intergenic
AGGATTTATTGTGGCAGCAC+TGG	0.460591	5.4:-71624203	MS.gene038271:CDS
AGTCCTTCTCTGCTTTCTTG+TGG	0.462235	5.4:-71624529	MS.gene038271:CDS
GGTTGCCGAAGCCGCTTCAA+TGG	0.468588	5.4:-71624501	MS.gene038271:CDS
GCTCTACATGCACCTTCCTA+AGG	0.469205	5.4:-71624012	MS.gene038271:CDS
CTCTGCCAATGCAAGAGCAC+TGG	0.470684	5.4:-71625551	MS.gene038271:CDS
GTGACAGAGGAGCATTTCTT+GGG	0.471825	5.4:-71624272	MS.gene038271:CDS
TCCAAGATGTGTATTGGAAA+AGG	0.474951	5.4:-71625618	None:intergenic
TTTCTTTAGACCAATCTCTT+GGG	0.478661	5.4:-71623955	MS.gene038271:CDS
GCCCTAAAAGGAATGGTGAT+TGG	0.491204	5.4:-71624037	MS.gene038271:CDS
ATGAGGTAATCCCAAGAGAT+TGG	0.494084	5.4:+71623945	None:intergenic
TATTACCAACAAAGAAATAG+TGG	0.495558	5.4:-71625580	MS.gene038271:CDS
TCAGATTCAGGTTCAGGTGG+TGG	0.495798	5.4:+71624674	None:intergenic
GGCACCCACCATAACATTGT+GGG	0.501925	5.4:-71625444	MS.gene038271:CDS
AAACATGTTTCTTGTATCAT+GGG	0.504377	5.4:-71624840	MS.gene038271:CDS
GTCATAAATTGAAGGATGGA+TGG	0.511206	5.4:+71624724	None:intergenic
GCATCGACCAGTTCCAGGAT+TGG	0.511694	5.4:-71624228	MS.gene038271:CDS
GGCAGCACTGGTGGAGAAGA+AGG	0.512702	5.4:-71624191	MS.gene038271:CDS
AATAAGCCTGTGATTACTAC+TGG	0.513795	5.4:-71625465	MS.gene038271:CDS
TTCTGCTAATCTACCACCAT+AGG	0.514784	5.4:+71624358	None:intergenic
AATCCAGATGAATCTGTTGA+AGG	0.515928	5.4:-71625258	MS.gene038271:CDS
ACTACCACCATTGTTTGAGT+TGG	0.516006	5.4:+71624571	None:intergenic
TTGAAGGATGGATGGAAGGT+GGG	0.520996	5.4:+71624732	None:intergenic
ATCCAGATGAATCTGTTGAA+GGG	0.525156	5.4:-71625257	MS.gene038271:CDS
TCACTTGCCAACTCAAACAA+TGG	0.528783	5.4:-71624578	MS.gene038271:CDS
ATTTATTGTGGCAGCACTGG+TGG	0.534671	5.4:-71624200	MS.gene038271:CDS
ACAGAGGTTTCCAGAGTCGA+AGG	0.536855	5.4:-71624144	MS.gene038271:CDS
ATTGAAGGATGGATGGAAGG+TGG	0.539276	5.4:+71624731	None:intergenic
TATCATGGGCCGAGATAGCT+CGG	0.539461	5.4:-71624826	MS.gene038271:CDS
AGAAGAAGGACCAACATTTG+TGG	0.543509	5.4:-71624177	MS.gene038271:CDS
TTGTTATTGTGCAGGCAACT+TGG	0.544767	5.4:-71624928	MS.gene038271:intron
GATTCAGGTTCAGGTGGTGG+AGG	0.546175	5.4:+71624677	None:intergenic
TATGCGACTTATTATTGGGT+CGG	0.548443	5.4:+71624637	None:intergenic
GATGGAAGGTGGGCGTCGTG+GGG	0.548673	5.4:+71624742	None:intergenic
AACACAAACTTTAATTGATG+AGG	0.548741	5.4:+71623928	None:intergenic
AGTCATCACTTCACCAGCAA+AGG	0.556336	5.4:+71625377	None:intergenic
AGAAGTGACCGTCGAAAACA+AGG	0.557353	5.4:+71624767	None:intergenic
CTTGGGTCTAATTGATTGGG+TGG	0.559612	5.4:-71624255	MS.gene038271:CDS
GGAAGCCAGTGCTCTTGCAT+TGG	0.564426	5.4:+71625546	None:intergenic
TGGTAGATTAGCAGAAGAGC+TGG	0.570353	5.4:-71624351	MS.gene038271:CDS
CGATTGTTGCAAAGTAACTC+AGG	0.570367	5.4:+71625339	None:intergenic
GGTTGATAATCCGCTATGCT+GGG	0.570935	5.4:-71624330	MS.gene038271:CDS
TAAATTGAAGGATGGATGGA+AGG	0.573429	5.4:+71624728	None:intergenic
AGAACACACCAACATCAACT+GGG	0.575989	5.4:+71625496	None:intergenic
CCTGAAGATACCACAAATGT+TGG	0.577043	5.4:+71624167	None:intergenic
AGAAACAAGAGCCTCACCTA+AGG	0.581520	5.4:+71625407	None:intergenic
TTTCCACAAGAAAGCAGAGA+AGG	0.586922	5.4:+71624526	None:intergenic
ACATACATGTCATAAATTGA+AGG	0.587593	5.4:+71624716	None:intergenic
GGTGGAGGTAGATCAGAGAC+GGG	0.587879	5.4:+71624692	None:intergenic
AACACCCCACAATGTTATGG+TGG	0.588442	5.4:+71625439	None:intergenic
TGGCACCCACCATAACATTG+TGG	0.591108	5.4:-71625445	MS.gene038271:CDS
ACATGTCATAAATTGAAGGA+TGG	0.593580	5.4:+71624720	None:intergenic
ATGCCAAGCCCTAAAAGGAA+TGG	0.595010	5.4:-71624044	MS.gene038271:CDS
TCTGCTAATCTACCACCATA+GGG	0.595034	5.4:+71624359	None:intergenic
TGGTGGAGGTAGATCAGAGA+CGG	0.600538	5.4:+71624691	None:intergenic
CTCTACATGCACCTTCCTAA+GGG	0.601014	5.4:-71624011	MS.gene038271:CDS
AGTGCAAGTGTCCCTTAGGA+AGG	0.602305	5.4:+71624000	None:intergenic
TTGTGGTTGACGGAAGAAAG+AGG	0.605169	5.4:-71624448	MS.gene038271:CDS
GAGTTACTTTGCAACAATCG+TGG	0.605999	5.4:-71625336	MS.gene038271:CDS
GACCAGTTCCAGGATTGGCA+AGG	0.609049	5.4:-71624223	MS.gene038271:CDS
AATGTGCTTTCGATTCCCTA+TGG	0.610089	5.4:-71624374	MS.gene038271:CDS
TTTGGAGGCGGCAGTGACAG+AGG	0.610364	5.4:-71624285	MS.gene038271:CDS
AATAGGCATCCGCTATGCGA+TGG	0.614428	5.4:+71624867	None:intergenic
AAGTAACTCAGGTTCACCCA+TGG	0.618644	5.4:+71625350	None:intergenic
GTGCTTTCGATTCCCTATGG+TGG	0.619185	5.4:-71624371	MS.gene038271:CDS
TGAAGTGATGACTAACTCCA+TGG	0.619717	5.4:-71625367	MS.gene038271:CDS
AAAATGGGGCTTGTGGTTGA+CGG	0.619932	5.4:-71624458	MS.gene038271:CDS
CTTGTTCCAAAGAAGAAACA+TGG	0.620389	5.4:-71625231	MS.gene038271:CDS
TGGGTGCCAGTAGTAATCAC+AGG	0.626482	5.4:+71625459	None:intergenic
ACACCCCACAATGTTATGGT+GGG	0.636435	5.4:+71625440	None:intergenic
TTGTGGTATCTTCAGGACAG+AGG	0.636511	5.4:-71624160	MS.gene038271:CDS
GGATTGGCAAGGATTTATTG+TGG	0.641507	5.4:-71624212	MS.gene038271:CDS
GAAATGTGGTGGCTTAGTAG+TGG	0.648560	5.4:-71624903	MS.gene038271:CDS
GAGTCAGATTCAGGTTCAGG+TGG	0.651262	5.4:+71624671	None:intergenic
GCACCCACCATAACATTGTG+GGG	0.654145	5.4:-71625443	MS.gene038271:CDS
AAATGATAAGATCCAAACAC+AGG	0.657847	5.4:+71624101	None:intergenic
GAACACACCAACATCAACTG+GGG	0.669825	5.4:+71625497	None:intergenic
GAGATTGGTCTAAAGAAATG+GGG	0.678557	5.4:+71623960	None:intergenic
AACACACCAACATCAACTGG+GGG	0.688247	5.4:+71625498	None:intergenic
CAGGCAACTTGGATGAAATG+TGG	0.695087	5.4:-71624917	MS.gene038271:CDS
CTTGCCAACTCAAACAATGG+TGG	0.697503	5.4:-71624575	MS.gene038271:CDS
TAGCCAGCACTACCTCCCCA+AGG	0.698597	5.4:+71624077	None:intergenic
GGAAGAAAGAGGCTAAGCAA+TGG	0.699803	5.4:-71624437	MS.gene038271:CDS
GAAGTGATGACTAACTCCAT+GGG	0.699827	5.4:-71625366	MS.gene038271:CDS
CGATCTGCCACCCAGCATAG+CGG	0.704547	5.4:+71624320	None:intergenic
TGATAATCCGCTATGCTGGG+TGG	0.706280	5.4:-71624327	MS.gene038271:CDS
GCAACTTGGATGAAATGTGG+TGG	0.707819	5.4:-71624914	MS.gene038271:CDS
GGGACACTTGCACTTCATGG+AGG	0.714604	5.4:-71623991	MS.gene038271:CDS
GTGGAGGTAGATCAGAGACG+GGG	0.788848	5.4:+71624693	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTAAAATAGTATTAATTTCA+GGG	-	chr5.4:71624358-71624377	MS.gene038271:CDS	15.0%
!!!	AGATTATGAATACATATTTT+GGG	-	chr5.4:71625153-71625172	MS.gene038271:intron	15.0%
!!!	ATAATACTAATTCTTTTGTA+AGG	+	chr5.4:71624584-71624603	None:intergenic	15.0%
!!!	TAATACTAATTCTTTTGTAA+GGG	+	chr5.4:71624583-71624602	None:intergenic	15.0%
!!	GGTAAAATAGTATTAATTTC+AGG	-	chr5.4:71624357-71624376	MS.gene038271:CDS	20.0%
!!!	GAGATTATGAATACATATTT+TGG	-	chr5.4:71625152-71625171	MS.gene038271:intron	20.0%
!!!	TAAACATAGAAACTTTTTCT+TGG	-	chr5.4:71624420-71624439	MS.gene038271:CDS	20.0%
!	AAAACATTGTTGCAAAAATG+GGG	-	chr5.4:71625078-71625097	MS.gene038271:intron	25.0%
!	AAACATGTTTCTTGTATCAT+GGG	-	chr5.4:71624710-71624729	MS.gene038271:CDS	25.0%
!	ACATACATGTCATAAATTGA+AGG	+	chr5.4:71624837-71624856	None:intergenic	25.0%
!	AGTATATGCGACTTATTATT+GGG	+	chr5.4:71624920-71624939	None:intergenic	25.0%
!	GAAAACATTGTTGCAAAAAT+GGG	-	chr5.4:71625077-71625096	MS.gene038271:intron	25.0%
!	TAGTATATGCGACTTATTAT+TGG	+	chr5.4:71624921-71624940	None:intergenic	25.0%
!	TATTACCAACAAAGAAATAG+TGG	-	chr5.4:71623970-71623989	MS.gene038271:CDS	25.0%
!	TGAAAACATTGTTGCAAAAA+TGG	-	chr5.4:71625076-71625095	MS.gene038271:intron	25.0%
!!	AATTATCATTGTTATTGTGC+AGG	-	chr5.4:71624614-71624633	MS.gene038271:CDS	25.0%
!!!	ATCAAAGACTTGTTATCATT+GGG	+	chr5.4:71624750-71624769	None:intergenic	25.0%
	AAATGATAAGATCCAAACAC+AGG	+	chr5.4:71625452-71625471	None:intergenic	30.0%
	AAGAAACATGTTTGCAGAAT+AGG	+	chr5.4:71624703-71624722	None:intergenic	30.0%
	ACATGTCATAAATTGAAGGA+TGG	+	chr5.4:71624833-71624852	None:intergenic	30.0%
	AGGCAAAGAATTATCATCAT+CGG	-	chr5.4:71624526-71624545	MS.gene038271:CDS	30.0%
	ATTTCTTGGGTCTAATTGAT+TGG	-	chr5.4:71625291-71625310	MS.gene038271:CDS	30.0%
	ATTTCTTTAGACCAATCTCT+TGG	-	chr5.4:71625594-71625613	MS.gene038271:CDS	30.0%
	CAAACATGTTTCTTGTATCA+TGG	-	chr5.4:71624709-71624728	MS.gene038271:CDS	30.0%
	TTAATATCAGAGTCAGATTC+AGG	+	chr5.4:71624891-71624910	None:intergenic	30.0%
	TTTCTTGGGTCTAATTGATT+GGG	-	chr5.4:71625292-71625311	MS.gene038271:CDS	30.0%
	TTTCTTTAGACCAATCTCTT+GGG	-	chr5.4:71625595-71625614	MS.gene038271:CDS	30.0%
	TTTGAAAAATTCCTTAGGTG+AGG	-	chr5.4:71624132-71624151	MS.gene038271:CDS	30.0%
!	AGATCGAGTTCATGAATTTT+TGG	-	chr5.4:71625247-71625266	MS.gene038271:CDS	30.0%
!	GTTTTCATTATCCATTGAAG+CGG	+	chr5.4:71625063-71625082	None:intergenic	30.0%
!	TAGATATCGACCAAACATTT+TGG	-	chr5.4:71624483-71624502	MS.gene038271:CDS	30.0%
!	TGGATCTTATCATTTTCCTT+GGG	-	chr5.4:71625457-71625476	MS.gene038271:CDS	30.0%
!	TTGGATCTTATCATTTTCCT+TGG	-	chr5.4:71625456-71625475	MS.gene038271:CDS	30.0%
!!	AAGAGATTGGTCTAAAGAAA+TGG	+	chr5.4:71625595-71625614	None:intergenic	30.0%
!!	AGAGATTGGTCTAAAGAAAT+GGG	+	chr5.4:71625594-71625613	None:intergenic	30.0%
!!	GATCAAAGACTTGTTATCAT+TGG	+	chr5.4:71624751-71624770	None:intergenic	30.0%
	AATAAGCCTGTGATTACTAC+TGG	-	chr5.4:71624085-71624104	MS.gene038271:CDS	35.0%
	AATCCAGATGAATCTGTTGA+AGG	-	chr5.4:71624292-71624311	MS.gene038271:CDS	35.0%
	AGTACACCATGTTTCTTCTT+TGG	+	chr5.4:71624328-71624347	None:intergenic	35.0%
	ATCCAGATGAATCTGTTGAA+GGG	-	chr5.4:71624293-71624312	MS.gene038271:CDS	35.0%
	ATTAGGTCAAGGTTTGATAG+TGG	+	chr5.4:71624028-71624047	None:intergenic	35.0%
	CTTGTTCCAAAGAAGAAACA+TGG	-	chr5.4:71624319-71624338	MS.gene038271:CDS	35.0%
	GAAAACAAGGTTGCATTGTT+GGG	+	chr5.4:71624773-71624792	None:intergenic	35.0%
	GGGTGTTTGAAAAATTCCTT+AGG	-	chr5.4:71624127-71624146	MS.gene038271:CDS	35.0%
	GTCATAAATTGAAGGATGGA+TGG	+	chr5.4:71624829-71624848	None:intergenic	35.0%
	GTTTCTTATTATGCCTTTGC+TGG	-	chr5.4:71624160-71624179	MS.gene038271:CDS	35.0%
	TAAATTGAAGGATGGATGGA+AGG	+	chr5.4:71624825-71624844	None:intergenic	35.0%
	TATGCGACTTATTATTGGGT+CGG	+	chr5.4:71624916-71624935	None:intergenic	35.0%
	TCTCGAGGGAAAATTAGAAT+AGG	-	chr5.4:71624506-71624525	MS.gene038271:CDS	35.0%
!	AACCAATCACCATTCCTTTT+AGG	+	chr5.4:71625518-71625537	None:intergenic	35.0%
!	ACCAATCACCATTCCTTTTA+GGG	+	chr5.4:71625517-71625536	None:intergenic	35.0%
!	AGTTTTTAAACTTGTGCCCT+TGG	+	chr5.4:71624401-71624420	None:intergenic	35.0%
!	GGATCTTATCATTTTCCTTG+GGG	-	chr5.4:71625458-71625477	MS.gene038271:CDS	35.0%
!	GTTTTTAAACTTGTGCCCTT+GGG	+	chr5.4:71624400-71624419	None:intergenic	35.0%
!	TTTTTAAACTTGTGCCCTTG+GGG	+	chr5.4:71624399-71624418	None:intergenic	35.0%
!!	AGTTCATGAATTTTTGGAGG+CGG	-	chr5.4:71625253-71625272	MS.gene038271:CDS	35.0%
!!	GAGATTGGTCTAAAGAAATG+GGG	+	chr5.4:71625593-71625612	None:intergenic	35.0%
!!	TCGAGTTCATGAATTTTTGG+AGG	-	chr5.4:71625250-71625269	MS.gene038271:CDS	35.0%
	AAGAACACACCAACATCAAC+TGG	+	chr5.4:71624058-71624077	None:intergenic	40.0%
	AATGTGCTTTCGATTCCCTA+TGG	-	chr5.4:71625176-71625195	MS.gene038271:intron	40.0%
	ACTACCACCATTGTTTGAGT+TGG	+	chr5.4:71624982-71625001	None:intergenic	40.0%
	AGAACACACCAACATCAACT+GGG	+	chr5.4:71624057-71624076	None:intergenic	40.0%
	AGAAGAAGGACCAACATTTG+TGG	-	chr5.4:71625373-71625392	MS.gene038271:CDS	40.0%
	ATGAGGTAATCCCAAGAGAT+TGG	+	chr5.4:71625608-71625627	None:intergenic	40.0%
	ATTATCCATTGAAGCGGCTT+CGG	+	chr5.4:71625057-71625076	None:intergenic	40.0%
	CAATAAATCCTTGCCAATCC+TGG	+	chr5.4:71625338-71625357	None:intergenic	40.0%
	CCAACATTTGTGGTATCTTC+AGG	-	chr5.4:71625383-71625402	MS.gene038271:CDS	40.0%
	CCTGAAGATACCACAAATGT+TGG	+	chr5.4:71625386-71625405	None:intergenic	40.0%
	CGAAAACAAGGTTGCATTGT+TGG	+	chr5.4:71624774-71624793	None:intergenic	40.0%
	CGATTGTTGCAAAGTAACTC+AGG	+	chr5.4:71624214-71624233	None:intergenic	40.0%
	CTCTGCTTTCTTGTGGAAAA+TGG	-	chr5.4:71625028-71625047	MS.gene038271:intron	40.0%
	GAAGTGATGACTAACTCCAT+GGG	-	chr5.4:71624184-71624203	MS.gene038271:CDS	40.0%
	TCAAACACCCCACAATGTTA+TGG	+	chr5.4:71624117-71624136	None:intergenic	40.0%
	TCACTTGCCAACTCAAACAA+TGG	-	chr5.4:71624972-71624991	MS.gene038271:intron	40.0%
	TCTGCTAATCTACCACCATA+GGG	+	chr5.4:71625194-71625213	None:intergenic	40.0%
	TGAAGTGATGACTAACTCCA+TGG	-	chr5.4:71624183-71624202	MS.gene038271:CDS	40.0%
	TTCCCTTCAACAGATTCATC+TGG	+	chr5.4:71624298-71624317	None:intergenic	40.0%
	TTCTGCTAATCTACCACCAT+AGG	+	chr5.4:71625195-71625214	None:intergenic	40.0%
	TTTCCACAAGAAAGCAGAGA+AGG	+	chr5.4:71625027-71625046	None:intergenic	40.0%
!	AGCAGCCACTATTTCTTTGT+TGG	+	chr5.4:71623978-71623997	None:intergenic	40.0%
!	GAGTTACTTTGCAACAATCG+TGG	-	chr5.4:71624214-71624233	MS.gene038271:CDS	40.0%
!	GGATTGGCAAGGATTTATTG+TGG	-	chr5.4:71625338-71625357	MS.gene038271:CDS	40.0%
!	TCTTATCATTTTCCTTGGGG+AGG	-	chr5.4:71625461-71625480	MS.gene038271:CDS	40.0%
!	TTGTTATTGTGCAGGCAACT+TGG	-	chr5.4:71624622-71624641	MS.gene038271:CDS	40.0%
!!	AAATGTTAACTTGTGCCCCA+AGG	-	chr5.4:71624381-71624400	MS.gene038271:CDS	40.0%
!!	AATGTTAACTTGTGCCCCAA+GGG	-	chr5.4:71624382-71624401	MS.gene038271:CDS	40.0%
!!	ACCAAACATTTTGGTCTCGA+GGG	-	chr5.4:71624492-71624511	MS.gene038271:CDS	40.0%
!!	AGTCGAAGGTTGATTTTGGA+TGG	-	chr5.4:71625420-71625439	MS.gene038271:CDS	40.0%
!!	GTCGAAGGTTGATTTTGGAT+GGG	-	chr5.4:71625421-71625440	MS.gene038271:CDS	40.0%
!!	TCGAAGGTTGATTTTGGATG+GGG	-	chr5.4:71625422-71625441	MS.gene038271:CDS	40.0%
!!!	CAATGCAACCTTGTTTTCGA+CGG	-	chr5.4:71624775-71624794	MS.gene038271:CDS	40.0%
	AAAATGGGGCTTGTGGTTGA+CGG	-	chr5.4:71625092-71625111	MS.gene038271:intron	45.0%
	AACACACCAACATCAACTGG+GGG	+	chr5.4:71624055-71624074	None:intergenic	45.0%
	AACACCCCACAATGTTATGG+TGG	+	chr5.4:71624114-71624133	None:intergenic	45.0%
	AAGTAACTCAGGTTCACCCA+TGG	+	chr5.4:71624203-71624222	None:intergenic	45.0%
	ACACCCCACAATGTTATGGT+GGG	+	chr5.4:71624113-71624132	None:intergenic	45.0%
	AGAAACAAGAGCCTCACCTA+AGG	+	chr5.4:71624146-71624165	None:intergenic	45.0%
	AGAAGTGACCGTCGAAAACA+AGG	+	chr5.4:71624786-71624805	None:intergenic	45.0%
	AGGATTTATTGTGGCAGCAC+TGG	-	chr5.4:71625347-71625366	MS.gene038271:CDS	45.0%
	AGTCATCACTTCACCAGCAA+AGG	+	chr5.4:71624176-71624195	None:intergenic	45.0%
	AGTCCTTCTCTGCTTTCTTG+TGG	-	chr5.4:71625021-71625040	MS.gene038271:intron	45.0%
	ATGAAGTGCAAGTGTCCCTT+AGG	+	chr5.4:71625557-71625576	None:intergenic	45.0%
	ATGCCAAGCCCTAAAAGGAA+TGG	-	chr5.4:71625506-71625525	MS.gene038271:CDS	45.0%
	ATTGAAGGATGGATGGAAGG+TGG	+	chr5.4:71624822-71624841	None:intergenic	45.0%
	CAGGCAACTTGGATGAAATG+TGG	-	chr5.4:71624633-71624652	MS.gene038271:CDS	45.0%
	CATCAACTGGGGGAAGAATT+AGG	+	chr5.4:71624045-71624064	None:intergenic	45.0%
	CCAAAATCAACCTTCGACTC+TGG	+	chr5.4:71625419-71625438	None:intergenic	45.0%
	CTCTACATGCACCTTCCTAA+GGG	-	chr5.4:71625539-71625558	MS.gene038271:CDS	45.0%
	CTTGCCAACTCAAACAATGG+TGG	-	chr5.4:71624975-71624994	MS.gene038271:intron	45.0%
	CTTGGGTCTAATTGATTGGG+TGG	-	chr5.4:71625295-71625314	MS.gene038271:CDS	45.0%
	GAAATGTGGTGGCTTAGTAG+TGG	-	chr5.4:71624647-71624666	MS.gene038271:CDS	45.0%
	GAACACACCAACATCAACTG+GGG	+	chr5.4:71624056-71624075	None:intergenic	45.0%
	GCAACTTGGATGAAATGTGG+TGG	-	chr5.4:71624636-71624655	MS.gene038271:CDS	45.0%
	GCCCTAAAAGGAATGGTGAT+TGG	-	chr5.4:71625513-71625532	MS.gene038271:CDS	45.0%
	GGAAGAAAGAGGCTAAGCAA+TGG	-	chr5.4:71625113-71625132	MS.gene038271:intron	45.0%
	TAAGGGACACTTGCACTTCA+TGG	-	chr5.4:71625556-71625575	MS.gene038271:CDS	45.0%
	TCCCTCGAGACCAAAATGTT+TGG	+	chr5.4:71624496-71624515	None:intergenic	45.0%
	TGGTAGATTAGCAGAAGAGC+TGG	-	chr5.4:71625199-71625218	MS.gene038271:intron	45.0%
	TGTTGCAAAAATGGGGCTTG+TGG	-	chr5.4:71625085-71625104	MS.gene038271:intron	45.0%
	TTGAAGGATGGATGGAAGGT+GGG	+	chr5.4:71624821-71624840	None:intergenic	45.0%
	TTGTGGTATCTTCAGGACAG+AGG	-	chr5.4:71625390-71625409	MS.gene038271:CDS	45.0%
	TTGTGGTTGACGGAAGAAAG+AGG	-	chr5.4:71625102-71625121	MS.gene038271:intron	45.0%
!	ATTCTTCCCCCAGTTGATGT+TGG	-	chr5.4:71624046-71624065	MS.gene038271:CDS	45.0%
!	ATTTATTGTGGCAGCACTGG+TGG	-	chr5.4:71625350-71625369	MS.gene038271:CDS	45.0%
!	CACTTTTGACCATCGCATAG+CGG	-	chr5.4:71624674-71624693	MS.gene038271:CDS	45.0%
!	TCAGAGTCAGATTCAGGTTC+AGG	+	chr5.4:71624885-71624904	None:intergenic	45.0%
!!	ACATGGTGTACTTGCTGTTC+AGG	-	chr5.4:71624336-71624355	MS.gene038271:CDS	45.0%
!!	AGTGACAGAGGAGCATTTCT+TGG	-	chr5.4:71625277-71625296	MS.gene038271:CDS	45.0%
!!	CCAGAGTCGAAGGTTGATTT+TGG	-	chr5.4:71625416-71625435	MS.gene038271:CDS	45.0%
!!	CGAAGGTTGATTTTGGATGG+GGG	-	chr5.4:71625423-71625442	MS.gene038271:CDS	45.0%
!!	GACCAAACATTTTGGTCTCG+AGG	-	chr5.4:71624491-71624510	MS.gene038271:CDS	45.0%
!!	GGTTGATAATCCGCTATGCT+GGG	-	chr5.4:71625220-71625239	MS.gene038271:intron	45.0%
!!	GTGACAGAGGAGCATTTCTT+GGG	-	chr5.4:71625278-71625297	MS.gene038271:CDS	45.0%
!!	TGGTTGATAATCCGCTATGC+TGG	-	chr5.4:71625219-71625238	MS.gene038271:intron	45.0%
!!!	TCACCATTCCTTTTAGGGCT+TGG	+	chr5.4:71625512-71625531	None:intergenic	45.0%
!!!	TTCCTTTTAGGGCTTGGCAT+TGG	+	chr5.4:71625506-71625525	None:intergenic	45.0%
	AATAGGCATCCGCTATGCGA+TGG	+	chr5.4:71624686-71624705	None:intergenic	50.0%
	ACAGAGGTTTCCAGAGTCGA+AGG	-	chr5.4:71625406-71625425	MS.gene038271:CDS	50.0%
	AGTGCAAGTGTCCCTTAGGA+AGG	+	chr5.4:71625553-71625572	None:intergenic	50.0%
	ATCATTGGGCCGAGCTATCT+CGG	+	chr5.4:71624736-71624755	None:intergenic	50.0%
	ATCCTTGCCAATCCTGGAAC+TGG	+	chr5.4:71625332-71625351	None:intergenic	50.0%
	CTGGGGGAAGAATTAGGTCA+AGG	+	chr5.4:71624039-71624058	None:intergenic	50.0%
	GCACCCACCATAACATTGTG+GGG	-	chr5.4:71624107-71624126	MS.gene038271:CDS	50.0%
	GCTCTACATGCACCTTCCTA+AGG	-	chr5.4:71625538-71625557	MS.gene038271:CDS	50.0%
	GGCACCCACCATAACATTGT+GGG	-	chr5.4:71624106-71624125	MS.gene038271:CDS	50.0%
	GTGCTTTCGATTCCCTATGG+TGG	-	chr5.4:71625179-71625198	MS.gene038271:intron	50.0%
	TATCATGGGCCGAGATAGCT+CGG	-	chr5.4:71624724-71624743	MS.gene038271:CDS	50.0%
	TGATAATCCGCTATGCTGGG+TGG	-	chr5.4:71625223-71625242	MS.gene038271:intron	50.0%
	TGCCAATGCCAAGCCCTAAA+AGG	-	chr5.4:71625501-71625520	MS.gene038271:CDS	50.0%
	TGGCACCCACCATAACATTG+TGG	-	chr5.4:71624105-71624124	MS.gene038271:CDS	50.0%
	TGGGTGCCAGTAGTAATCAC+AGG	+	chr5.4:71624094-71624113	None:intergenic	50.0%
	TGGTGGAGGTAGATCAGAGA+CGG	+	chr5.4:71624862-71624881	None:intergenic	50.0%
!	GAGTCAGATTCAGGTTCAGG+TGG	+	chr5.4:71624882-71624901	None:intergenic	50.0%
!	TCAGATTCAGGTTCAGGTGG+TGG	+	chr5.4:71624879-71624898	None:intergenic	50.0%
	GGAAGCCAGTGCTCTTGCAT+TGG	+	chr5.4:71624007-71624026	None:intergenic	55.0%
	GGGACACTTGCACTTCATGG+AGG	-	chr5.4:71625559-71625578	MS.gene038271:CDS	55.0%
	GGTGGAGGTAGATCAGAGAC+GGG	+	chr5.4:71624861-71624880	None:intergenic	55.0%
	GTGGAGGTAGATCAGAGACG+GGG	+	chr5.4:71624860-71624879	None:intergenic	55.0%
	TTTCCTTGGGGAGGTAGTGC+TGG	-	chr5.4:71625470-71625489	MS.gene038271:CDS	55.0%
!	CTCTGCCAATGCAAGAGCAC+TGG	-	chr5.4:71623999-71624018	MS.gene038271:CDS	55.0%
!	GACCAGTTCCAGGATTGGCA+AGG	-	chr5.4:71625327-71625346	MS.gene038271:CDS	55.0%
!	GATTCAGGTTCAGGTGGTGG+AGG	+	chr5.4:71624876-71624895	None:intergenic	55.0%
!	GCATCGACCAGTTCCAGGAT+TGG	-	chr5.4:71625322-71625341	MS.gene038271:CDS	55.0%
!!	GAAGAGCATCGACCAGTTCC+AGG	-	chr5.4:71625317-71625336	MS.gene038271:CDS	55.0%
!!!	GGACTCGAGTTTTGAGCGCT+TGG	+	chr5.4:71625006-71625025	None:intergenic	55.0%
	CGATCTGCCACCCAGCATAG+CGG	+	chr5.4:71625233-71625252	None:intergenic	60.0%
	GGATGGGGGAAGCCTGTGTT+TGG	-	chr5.4:71625437-71625456	MS.gene038271:CDS	60.0%
	GGTTGCCGAAGCCGCTTCAA+TGG	-	chr5.4:71625049-71625068	MS.gene038271:intron	60.0%
	TAGCCAGCACTACCTCCCCA+AGG	+	chr5.4:71625476-71625495	None:intergenic	60.0%
	TTTGGAGGCGGCAGTGACAG+AGG	-	chr5.4:71625265-71625284	MS.gene038271:CDS	60.0%
!!	GGCAGCACTGGTGGAGAAGA+AGG	-	chr5.4:71625359-71625378	MS.gene038271:CDS	60.0%
!	GATGGAAGGTGGGCGTCGTG+GGG	+	chr5.4:71624811-71624830	None:intergenic	65.0%
!	GGATGGAAGGTGGGCGTCGT+GGG	+	chr5.4:71624812-71624831	None:intergenic	65.0%
!	TGGATGGAAGGTGGGCGTCG+TGG	+	chr5.4:71624813-71624832	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	71623938	71625634	71623938	ID=MS.gene038271
chr5.4	mRNA	71623938	71625634	71623938	ID=MS.gene038271.t1;Parent=MS.gene038271
chr5.4	exon	71625230	71625634	71625230	ID=MS.gene038271.t1.exon1;Parent=MS.gene038271.t1
chr5.4	CDS	71625230	71625634	71625230	ID=cds.MS.gene038271.t1;Parent=MS.gene038271.t1
chr5.4	exon	71623938	71624936	71623938	ID=MS.gene038271.t1.exon2;Parent=MS.gene038271.t1
chr5.4	CDS	71623938	71624936	71623938	ID=cds.MS.gene038271.t1;Parent=MS.gene038271.t1

Gene Sequence

>MS.gene038271

ATGTGTATTGGAAAAGGAGAATTCACAGTGAATATTACCAACAAAGAAATAGTGGCTGCTGCTCTGCCAATGCAAGAGCACTGGCTTCCACTATCAAACCTTGACCTAATTCTTCCCCCAGTTGATGTTGGTGTGTTCTTTTGCTACAATAAGCCTGTGATTACTACTGGCACCCACCATAACATTGTGGGGTGTTTGAAAAATTCCTTAGGTGAGGCTCTTGTTTCTTATTATGCCTTTGCTGGTGAAGTGATGACTAACTCCATGGGTGAACCTGAGTTACTTTGCAACAATCGTGGAGTTGATTTTGTCGAAGCTTTTGCTGATGTTGAGTTACAATCTCTCAATTTGTATAATCCAGATGAATCTGTTGAAGGGAAGCTTGTTCCAAAGAAGAAACATGGTGTACTTGCTGTTCAGGTAAAATAGTATTAATTTCAGGGAAATGTTAACTTGTGCCCCAAGGGCACAAGTTTAAAAACTAAACATAGAAACTTTTTCTTGGAAACTGTACATTCATTGTATTAAAAGTTTAAAATTTTTAATAGATATCGACCAAACATTTTGGTCTCGAGGGAAAATTAGAATAGGCAAAGAATTATCATCATCGGCATGTAGTTTATTAATAATCATTAGTATTATCCCTTACAAAAGAATTAGTATTATAAAGTGTTTTAATTATCATTGTTATTGTGCAGGCAACTTGGATGAAATGTGGTGGCTTAGTAGTGGCTTGCACTTTTGACCATCGCATAGCGGATGCCTATTCTGCAAACATGTTTCTTGTATCATGGGCCGAGATAGCTCGGCCCAATGATAACAAGTCTTTGATCCCAACAATGCAACCTTGTTTTCGACGGTCACTTCTGACCCCACGACGCCCACCTTCCATCCATCCTTCAATTTATGACATGTATGTCCCCGTCTCTGATCTACCTCCACCACCTGAACCTGAATCTGACTCTGATATTAAAACCGACCCAATAATAAGTCGCATATACTATGTAACAAGCAAAGAACTCAACAACATGCAGTCACTTGCCAACTCAAACAATGGTGGTAGTTCCAAGCGCTCAAAACTCGAGTCCTTCTCTGCTTTCTTGTGGAAAATGGTTGCCGAAGCCGCTTCAATGGATAATGAAAACATTGTTGCAAAAATGGGGCTTGTGGTTGACGGAAGAAAGAGGCTAAGCAATGGTGACAAAAACAAAGAAGAGATTATGAATACATATTTTGGGAATGTGCTTTCGATTCCCTATGGTGGTAGATTAGCAGAAGAGCTGGTTGATAATCCGCTATGCTGGGTGGCAGATCGAGTTCATGAATTTTTGGAGGCGGCAGTGACAGAGGAGCATTTCTTGGGTCTAATTGATTGGGTGGAAGAGCATCGACCAGTTCCAGGATTGGCAAGGATTTATTGTGGCAGCACTGGTGGAGAAGAAGGACCAACATTTGTGGTATCTTCAGGACAGAGGTTTCCAGAGTCGAAGGTTGATTTTGGATGGGGGAAGCCTGTGTTTGGATCTTATCATTTTCCTTGGGGAGGTAGTGCTGGCTATGTTATGCCAATGCCAAGCCCTAAAAGGAATGGTGATTGGTTGCTCTACATGCACCTTCCTAAGGGACACTTGCACTTCATGGAGGCTCAAGCTCCCCATTTCTTTAGACCAATCTCTTGGGATTACCTCATCAATTAA

Protein sequence

>MS.gene038271.t1

MCIGKGEFTVNITNKEIVAAALPMQEHWLPLSNLDLILPPVDVGVFFCYNKPVITTGTHHNIVGCLKNSLGEALVSYYAFAGEVMTNSMGEPELLCNNRGVDFVEAFADVELQSLNLYNPDESVEGKLVPKKKHGATWMKCGGLVVACTFDHRIADAYSANMFLVSWAEIARPNDNKSLIPTMQPCFRRSLLTPRRPPSIHPSIYDMYVPVSDLPPPPEPESDSDIKTDPIISRIYYVTSKELNNMQSLANSNNGGSSKRSKLESFSAFLWKMVAEAASMDNENIVAKMGLVVDGRKRLSNGDKNKEEIMNTYFGNVLSIPYGGRLAEELVDNPLCWVADRVHEFLEAAVTEEHFLGLIDWVEEHRPVPGLARIYCGSTGGEEGPTFVVSSGQRFPESKVDFGWGKPVFGSYHFPWGGSAGYVMPMPSPKRNGDWLLYMHLPKGHLHFMEAQAPHFFRPISWDYLIN