AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene038274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038274.t1 MTR_5g090880 93.658 473 30 0 1 473 1 473 0.0 902
MS.gene038274.t1 MTR_5g090830 80.591 474 88 3 1 473 1 471 0.0 797
MS.gene038274.t1 MTR_5g090810 79.114 474 95 3 1 473 1 471 0.0 784
MS.gene038274.t1 MTR_3g450710 63.048 479 158 7 1 471 1 468 0.0 585
MS.gene038274.t1 MTR_3g450690 61.250 480 169 6 1 471 1 472 0.0 582
MS.gene038274.t1 MTR_5g026320 35.955 445 256 12 29 471 39 456 1.75e-73 239
MS.gene038274.t1 MTR_4g007540 26.362 459 284 16 10 452 3 423 3.95e-35 136
MS.gene038274.t1 MTR_2g064495 25.974 462 289 16 10 456 3 426 4.74e-35 135
MS.gene038274.t1 MTR_2g105330 25.974 462 289 16 10 456 3 426 8.78e-35 135
MS.gene038274.t1 MTR_8g075610 25.221 452 288 15 17 456 1 414 1.43e-34 134
MS.gene038274.t1 MTR_8g096530 26.196 439 274 16 31 459 28 426 6.60e-30 121
MS.gene038274.t1 MTR_3g098980 27.000 400 242 15 26 414 29 389 1.24e-27 115
MS.gene038274.t1 MTR_3g049150 24.826 431 276 16 50 456 34 440 1.13e-26 112
MS.gene038274.t1 MTR_1g040215 26.119 402 234 13 64 437 61 427 1.42e-26 112
MS.gene038274.t1 MTR_7g114560 25.060 419 265 13 49 450 42 428 2.39e-26 111
MS.gene038274.t1 MTR_7g027175 26.241 423 263 17 4 415 9 393 7.51e-26 109
MS.gene038274.t1 MTR_7g114550 25.355 422 260 15 49 450 42 428 2.37e-25 108
MS.gene038274.t1 MTR_7g085990 26.562 384 232 15 61 414 49 412 5.49e-25 107
MS.gene038274.t1 MTR_7g086000 27.200 375 219 15 67 413 60 408 1.03e-24 107
MS.gene038274.t1 MTR_1g040175 25.061 411 239 14 64 443 61 433 3.36e-24 105
MS.gene038274.t1 MTR_4g127650 23.684 456 302 13 29 466 29 456 1.03e-23 103
MS.gene038274.t1 MTR_7g114490 24.419 430 272 15 49 459 42 437 4.63e-23 102
MS.gene038274.t1 MTR_7g114470 25.758 396 241 15 64 435 63 429 5.35e-23 101
MS.gene038274.t1 MTR_5g076700 26.173 405 238 17 67 450 65 429 5.73e-23 101
MS.gene038274.t1 MTR_4g097090 25.055 455 298 16 29 466 28 456 1.25e-22 100
MS.gene038274.t1 MTR_1g081660 25.373 402 253 17 64 452 70 437 2.68e-22 99.8
MS.gene038274.t1 MTR_7g114520 26.562 384 234 15 49 416 43 394 1.72e-21 97.4
MS.gene038274.t1 MTR_3g026950 24.429 438 289 12 29 447 21 435 4.55e-21 95.9
MS.gene038274.t1 MTR_7g085930 25.197 381 241 13 61 414 53 416 8.56e-21 95.1
MS.gene038274.t1 MTR_1g053315 24.020 408 251 17 29 414 40 410 2.96e-18 87.4
MS.gene038274.t1 MTR_7g084940 26.056 426 252 17 6 413 12 392 5.33e-18 86.7
MS.gene038274.t1 MTR_3g465990 25.000 412 254 15 49 440 50 426 1.77e-17 85.1
MS.gene038274.t1 MTR_1g040195 24.523 367 216 14 97 437 71 402 3.04e-17 84.3
MS.gene038274.t1 MTR_1g040320 22.794 408 216 14 64 443 61 397 3.05e-17 84.0
MS.gene038274.t1 MTR_7g084910 25.597 461 255 21 7 435 14 418 2.34e-16 81.6
MS.gene038274.t1 MTR_3g465980 25.519 337 211 10 49 369 50 362 2.38e-16 81.6
MS.gene038274.t1 MTR_1g040315 26.457 223 141 6 64 278 61 268 1.02e-15 79.3
MS.gene038274.t1 MTR_3g086020 25.000 396 235 19 31 417 28 370 1.45e-15 79.0
MS.gene038274.t1 MTR_4g055170 23.716 409 251 13 67 449 69 442 4.03e-15 77.8
MS.gene038274.t1 MTR_8g079980 22.879 389 238 12 43 414 38 381 5.02e-15 77.4
MS.gene038274.t1 MTR_3g112180 24.051 395 227 14 67 429 61 414 1.51e-14 75.9
MS.gene038274.t1 MTR_3g101900 23.760 383 230 14 67 425 66 410 3.79e-14 74.7
MS.gene038274.t1 MTR_2g015430 23.210 405 267 16 21 415 15 385 4.74e-14 74.3
MS.gene038274.t1 MTR_4g022720 26.087 368 217 14 67 414 55 387 6.76e-14 73.9
MS.gene038274.t1 MTR_8g467580 25.067 375 206 18 67 414 60 386 7.13e-14 73.9
MS.gene038274.t1 MTR_7g056087 23.301 412 259 16 63 453 54 429 2.70e-13 72.0
MS.gene038274.t1 MTR_3g088420 23.198 444 264 18 36 455 32 422 2.83e-13 72.0
MS.gene038274.t1 MTR_3g101930 23.873 377 223 14 62 412 65 403 1.44e-12 69.7
MS.gene038274.t1 MTR_4g022290 24.265 408 229 18 36 414 38 394 1.98e-12 69.3
MS.gene038274.t1 MTR_8g079950 21.465 396 258 11 31 413 26 381 2.10e-12 69.3
MS.gene038274.t1 MTR_8g041770 24.331 411 245 16 26 414 25 391 4.25e-12 68.2
MS.gene038274.t1 MTR_4g094828 22.472 445 278 16 36 455 37 439 1.04e-11 67.0
MS.gene038274.t1 MTR_6g053810 24.695 328 183 13 67 365 71 363 2.19e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038274.t1 AT1G78990 53.632 468 188 9 8 471 11 453 1.83e-160 462
MS.gene038274.t1 AT1G32910 54.371 469 188 9 8 471 11 458 6.13e-159 458
MS.gene038274.t1 AT5G16410 49.789 474 200 11 8 471 34 479 2.16e-148 432
MS.gene038274.t1 AT1G31490 35.639 477 254 15 8 471 4 440 4.58e-76 245
MS.gene038274.t1 AT5G48930 26.247 461 290 16 10 456 3 427 4.80e-35 135
MS.gene038274.t1 AT5G41040 26.329 395 245 13 31 415 38 396 2.64e-29 120
MS.gene038274.t1 AT5G41040 26.329 395 245 13 31 415 54 412 3.63e-29 119
MS.gene038274.t1 AT5G57840 25.054 463 289 15 10 452 3 427 9.92e-26 109
MS.gene038274.t1 AT3G03480 26.173 405 230 15 64 437 70 436 9.20e-24 103
MS.gene038274.t1 AT5G17540 26.042 384 233 14 45 416 46 390 1.05e-23 103
MS.gene038274.t1 AT5G63560 26.241 423 234 17 31 425 30 402 9.53e-23 100
MS.gene038274.t1 AT3G26040 26.190 378 228 14 52 414 39 380 1.02e-22 100
MS.gene038274.t1 AT3G48720 24.569 464 300 16 3 456 2 425 4.57e-22 98.6
MS.gene038274.t1 AT1G28680 27.518 407 244 20 23 415 19 388 7.15e-21 95.5
MS.gene038274.t1 AT1G65450 25.833 360 223 13 67 413 72 400 1.08e-20 94.7
MS.gene038274.t1 AT1G24430 27.089 395 230 17 31 414 29 376 1.25e-20 94.4
MS.gene038274.t1 AT2G40230 25.185 405 244 14 62 448 54 417 4.59e-20 92.8
MS.gene038274.t1 AT2G19070 23.251 443 293 15 43 466 37 451 2.54e-19 90.5
MS.gene038274.t1 AT1G27620 22.943 401 262 13 67 456 58 422 3.22e-19 90.1
MS.gene038274.t1 AT5G67160 25.814 430 245 19 64 473 53 428 3.24e-19 90.1
MS.gene038274.t1 AT5G47950 23.583 441 266 15 29 456 27 409 1.24e-16 82.4
MS.gene038274.t1 AT3G30280 26.238 404 240 18 27 414 25 386 2.45e-16 81.6
MS.gene038274.t1 AT4G15390 26.761 284 154 11 27 292 27 274 4.15e-15 77.8
MS.gene038274.t1 AT4G15390 26.761 284 154 11 27 292 27 274 4.15e-15 77.8
MS.gene038274.t1 AT5G23970 23.765 425 263 15 27 439 20 395 3.26e-14 74.7
MS.gene038274.t1 AT5G67150 24.397 373 228 15 63 414 57 396 7.14e-14 73.9
MS.gene038274.t1 AT5G38130 22.704 392 243 14 61 430 73 426 1.01e-13 73.6
MS.gene038274.t1 AT1G24420 24.691 405 235 14 29 414 26 379 1.13e-13 73.2
MS.gene038274.t1 AT1G24420 24.691 405 235 14 29 414 26 379 1.13e-13 73.2
MS.gene038274.t1 AT2G25150 23.626 364 238 11 64 414 64 400 1.44e-12 70.1
MS.gene038274.t1 AT5G42830 22.667 375 237 15 61 415 58 399 2.46e-11 65.9
MS.gene038274.t1 AT3G50300 23.118 372 231 14 64 414 56 393 2.51e-11 65.9

Find 127 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAGTCAAAGGTAGATTT+TGG 0.197134 5.4:-71646386 MS.gene038274:CDS
GGATGGAAGGTGGGTGTCTT+GGG 0.229374 5.4:+71646993 None:intergenic
TGCAACAACATTTCCACTAT+TGG 0.271768 5.4:+71646733 None:intergenic
CCGACATTTGTGGTGTCTTC+TGG 0.272565 5.4:-71646419 MS.gene038274:CDS
GAGTTTATGAGTTCGTATTT+TGG 0.286533 5.4:-71646650 MS.gene038274:CDS
TCTGATTCGGGCTTGGGTTC+AGG 0.310598 5.4:+71646920 None:intergenic
GTTTCCTATTACGTCTTTGC+TGG 0.319072 5.4:-71647512 MS.gene038274:CDS
TTAATATCTGATTCGGGCTT+GGG 0.319670 5.4:+71646914 None:intergenic
TTTAATATCTGATTCGGGCT+TGG 0.321191 5.4:+71646913 None:intergenic
GAAGAGCATCGACCGGTTCC+AGG 0.324397 5.4:-71646485 MS.gene038274:CDS
TGGATGGAAGGTGGGTGTCT+TGG 0.324811 5.4:+71646992 None:intergenic
TAAGGAATACTTGCACTTCA+TGG 0.338830 5.4:-71646246 MS.gene038274:CDS
AGGATTTATTGCGGAGTTCC+TGG 0.341629 5.4:-71646455 MS.gene038274:CDS
ATTTCTTGGGACTAATTGAT+TGG 0.344863 5.4:-71646511 MS.gene038274:CDS
CAAACATGTTTCTTGTATCA+TGG 0.350145 5.4:-71647093 MS.gene038274:CDS
TTCTGCTTTCTTGTGGAAGA+TGG 0.357240 5.4:-71646774 MS.gene038274:CDS
TGCCAATGCCTAGTCCTAAA+AGG 0.369732 5.4:-71646301 MS.gene038274:CDS
ATGAGATTGGTCTAAAGAAA+TGG 0.371643 5.4:+71646210 None:intergenic
AAGAACACACCAATACTAAC+AGG 0.372400 5.4:+71647620 None:intergenic
AAGAAACATGTTTGTAGAAT+AGG 0.374201 5.4:+71647102 None:intergenic
GGACACGGAAGAACATTTCT+TGG 0.384547 5.4:-71646525 MS.gene038274:CDS
TTCCCTTCAATGGTTTCCTC+TGG 0.385000 5.4:+71647377 None:intergenic
TGGAACAAGCTTCCCTTCAA+TGG 0.386738 5.4:+71647367 None:intergenic
ACATGGTGTGCTTGCTGTTC+AGG 0.388373 5.4:-71647336 MS.gene038274:intron
ATTCCTTGTGGTGGTGATGC+TGG 0.393172 5.4:-71646332 MS.gene038274:CDS
TTACGTCTTTGCTGGTGAAA+TGG 0.405271 5.4:-71647504 MS.gene038274:CDS
TCGGGCTTGGGTTCAGGTGG+AGG 0.406362 5.4:+71646926 None:intergenic
GTGGGTGTCTTGGGGTCAAA+AGG 0.407163 5.4:+71647002 None:intergenic
AACAATCCTATGATTACTAT+TGG 0.407840 5.4:-71647590 MS.gene038274:CDS
TTCACCAGCAAAGACGTAAT+AGG 0.408583 5.4:+71647508 None:intergenic
CGCTCTGTAACCCAACATAA+TGG 0.415712 5.4:+71646572 None:intergenic
TGAGATTGGTCTAAAGAAAT+GGG 0.424402 5.4:+71646211 None:intergenic
CTAATCACTAAAATGAGTAT+TGG 0.430164 5.4:-71647749 None:intergenic
TGTTGAAAAGCCATTATGTT+GGG 0.431386 5.4:-71646582 MS.gene038274:CDS
CCCATGTTCTTGCATTAGCT+TGG 0.434231 5.4:+71647676 None:intergenic
CCAAAATCTACCTTTGACTC+TGG 0.435822 5.4:+71646386 None:intergenic
GCTTCCACTATTATTTGTGT+TGG 0.455348 5.4:+71646823 None:intergenic
CCCCACAATAGTGTTATTTG+TGG 0.461341 5.4:+71647565 None:intergenic
GACACGGAAGAACATTTCTT+GGG 0.462909 5.4:-71646524 MS.gene038274:CDS
ACCACAAATAACACTATTGT+GGG 0.465516 5.4:-71647566 MS.gene038274:CDS
TTTCTTGGGACTAATTGATT+GGG 0.478579 5.4:-71646510 MS.gene038274:CDS
TCTATTAATTTACAACTGTA+GGG 0.479476 5.4:+71646611 None:intergenic
ATGAAGTGCAAGTATTCCTT+AGG 0.482803 5.4:+71646248 None:intergenic
GGCTTGGGTTCAGGTGGAGG+AGG 0.482969 5.4:+71646929 None:intergenic
ATTGCTCGAGCGGGCTATCT+CGG 0.484103 5.4:+71647069 None:intergenic
ATTTCTTTAGACCAATCTCA+TGG 0.487418 5.4:-71646208 MS.gene038274:CDS
GTCCTAAAAGGAATGGTGAT+TGG 0.487820 5.4:-71646289 MS.gene038274:CDS
CAATAAATCCTTGCTAGCCC+TGG 0.488996 5.4:+71646467 None:intergenic
AGAAGGTAATCCCATGAGAT+TGG 0.494084 5.4:+71646197 None:intergenic
TTCTATTAATTTACAACTGT+AGG 0.494706 5.4:+71646610 None:intergenic
ATCCTTGCTAGCCCTGGAAC+CGG 0.495325 5.4:+71646473 None:intergenic
GTGGTGCCAATAGTAATCAT+AGG 0.495972 5.4:+71647584 None:intergenic
CGGTTGTTGCAAAGTAGCTC+AGG 0.499190 5.4:+71647461 None:intergenic
AAACATGTTTCTTGTATCAT+GGG 0.502267 5.4:-71647092 MS.gene038274:CDS
TCAGCCGCTTCAACCAATAG+TGG 0.504854 5.4:-71646746 MS.gene038274:CDS
CACCACAAATAACACTATTG+TGG 0.510410 5.4:-71647567 MS.gene038274:CDS
GGATCTTATCATATTCCTTG+TGG 0.513474 5.4:-71646344 MS.gene038274:CDS
ACCAAGCTAATGCAAGAACA+TGG 0.522741 5.4:-71647677 MS.gene038274:CDS
GATGGAAGGTGGGTGTCTTG+GGG 0.526099 5.4:+71646994 None:intergenic
CTAGCCAACACAAATAATAG+TGG 0.526578 5.4:-71646827 MS.gene038274:CDS
GTGAAGGATGGATGGAAGGT+GGG 0.534125 5.4:+71646984 None:intergenic
GTCATAGAGTGAAGGATGGA+TGG 0.535942 5.4:+71646976 None:intergenic
GACCGGTTCCAGGGCTAGCA+AGG 0.536048 5.4:-71646475 MS.gene038274:CDS
AAGCCAGAGGAAACCATTGA+AGG 0.538348 5.4:-71647380 MS.gene038274:CDS
GATTCGGGCTTGGGTTCAGG+TGG 0.543751 5.4:+71646923 None:intergenic
GATCTACATGAATCTTCCTA+AGG 0.548247 5.4:-71646264 MS.gene038274:CDS
TTGTTGAAAAGCCATTATGT+TGG 0.550086 5.4:-71646583 MS.gene038274:CDS
GTCGGTCCATCTGCTCCACC+AGG 0.550954 5.4:+71646437 None:intergenic
TGGGTGTCTTGGGGTCAAAA+GGG 0.554761 5.4:+71647003 None:intergenic
GTATAATTGTGCAGGCAACT+TGG 0.557460 5.4:-71647180 MS.gene038274:intron
AGTGAAGGATGGATGGAAGG+TGG 0.560310 5.4:+71646983 None:intergenic
TGGAGGAGGTAGATCCGAGA+TGG 0.563260 5.4:+71646943 None:intergenic
AATATGATAAGATCCAACTA+CGG 0.564107 5.4:+71646352 None:intergenic
TAGAGTGAAGGATGGATGGA+AGG 0.564472 5.4:+71646980 None:intergenic
CAGGCAACTTGGATGAAATG+TGG 0.566561 5.4:-71647169 MS.gene038274:CDS
ATTTCCACTATTGGTTGAAG+CGG 0.566759 5.4:+71646742 None:intergenic
CTTGGGACTAATTGATTGGG+TGG 0.567048 5.4:-71646507 MS.gene038274:CDS
AGACTTGTTATTGCTCGAGC+GGG 0.569461 5.4:+71647060 None:intergenic
ACAAAGATTTCCAGAGTCAA+AGG 0.570544 5.4:-71646396 MS.gene038274:CDS
GGAGTTCCTGGTGGAGCAGA+TGG 0.571944 5.4:-71646443 MS.gene038274:CDS
TGAAATGGTCACTAACTCCA+TGG 0.576847 5.4:-71647489 MS.gene038274:CDS
AGGCAACTTGGATGAAATGT+GGG 0.579164 5.4:-71647168 MS.gene038274:CDS
TAGTACATGCGACTTATTAG+CGG 0.580648 5.4:+71646884 None:intergenic
AGCCAGAGGAAACCATTGAA+GGG 0.581302 5.4:-71647379 MS.gene038274:CDS
AAAAGGGACCTCTGAAAACA+AGG 0.581419 5.4:+71647019 None:intergenic
AGTACATGCGACTTATTAGC+GGG 0.583367 5.4:+71646885 None:intergenic
TCTTATCATATTCCTTGTGG+TGG 0.583934 5.4:-71646341 MS.gene038274:CDS
TTTCTTTAGACCAATCTCAT+GGG 0.587261 5.4:-71646207 MS.gene038274:CDS
ATGCCTAGTCCTAAAAGGAA+TGG 0.590231 5.4:-71646296 MS.gene038274:CDS
ATATGATAAGATCCAACTAC+GGG 0.599022 5.4:+71646353 None:intergenic
GGAAACAAGAGCTTCAGCTA+AGG 0.599927 5.4:+71647529 None:intergenic
TGGCATGCAACTTTGACCAT+CGG 0.600034 5.4:-71647135 MS.gene038274:CDS
AAGACTTGTTATTGCTCGAG+CGG 0.603884 5.4:+71647059 None:intergenic
AAGTAGCTCAGGTTCACCCA+TGG 0.604674 5.4:+71647472 None:intergenic
AGCTGCAACTACCTTCTCGT+TGG 0.607766 5.4:+71647700 None:intergenic
ACTTTGCAACAACCGTGGTG+TGG 0.609718 5.4:-71647453 MS.gene038274:CDS
AAGAGCATCGACCGGTTCCA+GGG 0.612956 5.4:-71646484 MS.gene038274:CDS
TTGGGTGGAAGAGCATCGAC+CGG 0.614426 5.4:-71646492 MS.gene038274:CDS
AGCAGATGGACCGACATTTG+TGG 0.619293 5.4:-71646429 MS.gene038274:CDS
AATAGGCGTCCGCTATCCGA+TGG 0.620621 5.4:+71647119 None:intergenic
TGACATGTACATCTCCATCT+CGG 0.620929 5.4:-71646957 MS.gene038274:CDS
TGTGTTGGCTAGTAACTGCA+TGG 0.621948 5.4:+71646838 None:intergenic
ATGTACATGTCATAGAGTGA+AGG 0.625954 5.4:+71646968 None:intergenic
CAACTTTGACCATCGGATAG+CGG 0.635631 5.4:-71647128 MS.gene038274:CDS
CCAGAAGACACCACAAATGT+CGG 0.636633 5.4:+71646419 None:intergenic
CTTGTTCCAAAGAAAACACA+TGG 0.639769 5.4:-71647353 MS.gene038274:CDS
ATTTATTGCGGAGTTCCTGG+TGG 0.643183 5.4:-71646452 MS.gene038274:CDS
TGTCAATATTACCAACGAGA+AGG 0.643953 5.4:-71647711 MS.gene038274:CDS
CCAAGCTAATGCAAGAACAT+GGG 0.649131 5.4:-71647676 MS.gene038274:CDS
GAACACACCAATACTAACAG+GGG 0.649933 5.4:+71647622 None:intergenic
AAAATGGGGATTGTGGTCGA+CGG 0.658600 5.4:-71646710 MS.gene038274:CDS
GGAAGAATGAGATTAAGCAA+TGG 0.658733 5.4:-71646689 MS.gene038274:CDS
GCAACTTGGATGAAATGTGG+GGG 0.664532 5.4:-71647166 MS.gene038274:CDS
AGAACACACCAATACTAACA+GGG 0.664943 5.4:+71647621 None:intergenic
GGGCTAGCAAGGATTTATTG+CGG 0.668380 5.4:-71646464 MS.gene038274:CDS
TCTCAATTTGCATAAGCCAG+AGG 0.668470 5.4:-71647393 MS.gene038274:CDS
GAAATGGTCACTAACTCCAT+GGG 0.670922 5.4:-71647488 MS.gene038274:CDS
ACATGTCATAGAGTGAAGGA+TGG 0.670976 5.4:+71646972 None:intergenic
TAGCCAGCATCACCACCACA+AGG 0.673907 5.4:+71646329 None:intergenic
GGCAACTTGGATGAAATGTG+GGG 0.674719 5.4:-71647167 MS.gene038274:CDS
GAGATTGGTCTAAAGAAATG+GGG 0.682938 5.4:+71646212 None:intergenic
GGAATACTTGCACTTCATGG+AGG 0.690260 5.4:-71646243 MS.gene038274:CDS
CCACAAATAACACTATTGTG+GGG 0.693360 5.4:-71647565 MS.gene038274:CDS
AACACACCAATACTAACAGG+GGG 0.696942 5.4:+71647623 None:intergenic
GAGCTACTTTGCAACAACCG+TGG 0.705966 5.4:-71647458 MS.gene038274:CDS
CAACTTGGATGAAATGTGGG+GGG 0.712514 5.4:-71647165 MS.gene038274:CDS
CTTCAACAAAATCCACACCA+CGG 0.748079 5.4:+71647441 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATTATTATCATTATGC+AGG - chr5.4:71646682-71646701 MS.gene038274:CDS 20.0%
!! TCTATTAATTTACAACTGTA+GGG + chr5.4:71647319-71647338 None:intergenic 20.0%
!! TTCTATTAATTTACAACTGT+AGG + chr5.4:71647320-71647339 None:intergenic 20.0%
!!! GTTTGTTTTAATATCTGATT+CGG + chr5.4:71647023-71647042 None:intergenic 20.0%
!!! TTTGTTTTAATATCTGATTC+GGG + chr5.4:71647022-71647041 None:intergenic 20.0%
! AAACATGTTTCTTGTATCAT+GGG - chr5.4:71646835-71646854 MS.gene038274:CDS 25.0%
! AACAATCCTATGATTACTAT+TGG - chr5.4:71646337-71646356 MS.gene038274:CDS 25.0%
! AAGAAACATGTTTGTAGAAT+AGG + chr5.4:71646828-71646847 None:intergenic 25.0%
! AATATGATAAGATCCAACTA+CGG + chr5.4:71647578-71647597 None:intergenic 25.0%
! AATTGTATGTATAATTGTGC+AGG - chr5.4:71646739-71646758 MS.gene038274:CDS 25.0%
!! AGTTTATGAGTTCGTATTTT+GGG - chr5.4:71647278-71647297 MS.gene038274:intron 25.0%
!!! ACAAAAACATTTTGATCTCA+AGG - chr5.4:71646642-71646661 MS.gene038274:CDS 25.0%
!!! CAAAAACATTTTGATCTCAA+GGG - chr5.4:71646643-71646662 MS.gene038274:CDS 25.0%
ACCACAAATAACACTATTGT+GGG - chr5.4:71646361-71646380 MS.gene038274:CDS 30.0%
ATATGATAAGATCCAACTAC+GGG + chr5.4:71647577-71647596 None:intergenic 30.0%
ATTTCTTGGGACTAATTGAT+TGG - chr5.4:71647416-71647435 MS.gene038274:CDS 30.0%
ATTTCTTTAGACCAATCTCA+TGG - chr5.4:71647719-71647738 MS.gene038274:CDS 30.0%
CAAACATGTTTCTTGTATCA+TGG - chr5.4:71646834-71646853 MS.gene038274:CDS 30.0%
GAAATGTTGTTGCAAAAATG+GGG - chr5.4:71647203-71647222 MS.gene038274:intron 30.0%
GGAAATGTTGTTGCAAAAAT+GGG - chr5.4:71647202-71647221 MS.gene038274:intron 30.0%
TGGAAATGTTGTTGCAAAAA+TGG - chr5.4:71647201-71647220 MS.gene038274:intron 30.0%
TGTTGAAAAGCCATTATGTT+GGG - chr5.4:71647345-71647364 MS.gene038274:CDS 30.0%
TTGTTGAAAAGCCATTATGT+TGG - chr5.4:71647344-71647363 MS.gene038274:CDS 30.0%
TTTCTTGGGACTAATTGATT+GGG - chr5.4:71647417-71647436 MS.gene038274:CDS 30.0%
TTTCTTTAGACCAATCTCAT+GGG - chr5.4:71647720-71647739 MS.gene038274:CDS 30.0%
! AATCATTTTCTGCTTTCTTG+TGG - chr5.4:71647146-71647165 MS.gene038274:CDS 30.0%
! GAGTTTATGAGTTCGTATTT+TGG - chr5.4:71647277-71647296 MS.gene038274:intron 30.0%
!! ATGAGATTGGTCTAAAGAAA+TGG + chr5.4:71647720-71647739 None:intergenic 30.0%
!! TGAGATTGGTCTAAAGAAAT+GGG + chr5.4:71647719-71647738 None:intergenic 30.0%
AAGAACACACCAATACTAAC+AGG + chr5.4:71646310-71646329 None:intergenic 35.0%
ACAAAGATTTCCAGAGTCAA+AGG - chr5.4:71647531-71647550 MS.gene038274:CDS 35.0%
AGAACACACCAATACTAACA+GGG + chr5.4:71646309-71646328 None:intergenic 35.0%
ATGAAGTGCAAGTATTCCTT+AGG + chr5.4:71647682-71647701 None:intergenic 35.0%
ATGTACATGTCATAGAGTGA+AGG + chr5.4:71646962-71646981 None:intergenic 35.0%
CACCACAAATAACACTATTG+TGG - chr5.4:71646360-71646379 MS.gene038274:CDS 35.0%
CCACAAATAACACTATTGTG+GGG - chr5.4:71646362-71646381 MS.gene038274:CDS 35.0%
CTAGCCAACACAAATAATAG+TGG - chr5.4:71647100-71647119 MS.gene038274:CDS 35.0%
CTTGTTCCAAAGAAAACACA+TGG - chr5.4:71646574-71646593 MS.gene038274:CDS 35.0%
GATCTACATGAATCTTCCTA+AGG - chr5.4:71647663-71647682 MS.gene038274:CDS 35.0%
GCTTCCACTATTATTTGTGT+TGG + chr5.4:71647107-71647126 None:intergenic 35.0%
GGAAGAATGAGATTAAGCAA+TGG - chr5.4:71647238-71647257 MS.gene038274:intron 35.0%
GGATCTTATCATATTCCTTG+TGG - chr5.4:71647583-71647602 MS.gene038274:CDS 35.0%
TAAGGAATACTTGCACTTCA+TGG - chr5.4:71647681-71647700 MS.gene038274:CDS 35.0%
TAGTACATGCGACTTATTAG+CGG + chr5.4:71647046-71647065 None:intergenic 35.0%
TCTTATCATATTCCTTGTGG+TGG - chr5.4:71647586-71647605 MS.gene038274:CDS 35.0%
TGCAACAACATTTCCACTAT+TGG + chr5.4:71647197-71647216 None:intergenic 35.0%
TGTCAATATTACCAACGAGA+AGG - chr5.4:71646216-71646235 MS.gene038274:CDS 35.0%
! AACCAATCACCATTCCTTTT+AGG + chr5.4:71647643-71647662 None:intergenic 35.0%
! AGTCAAAGGTAGATTTTGGA+TGG - chr5.4:71647545-71647564 MS.gene038274:CDS 35.0%
! ATTTCCACTATTGGTTGAAG+CGG + chr5.4:71647188-71647207 None:intergenic 35.0%
! GTCAAAGGTAGATTTTGGAT+GGG - chr5.4:71647546-71647565 MS.gene038274:CDS 35.0%
! TCAAAGGTAGATTTTGGATG+GGG - chr5.4:71647547-71647566 MS.gene038274:CDS 35.0%
!! GAGATTGGTCTAAAGAAATG+GGG + chr5.4:71647718-71647737 None:intergenic 35.0%
!! TTAATATCTGATTCGGGCTT+GGG + chr5.4:71647016-71647035 None:intergenic 35.0%
!! TTTAATATCTGATTCGGGCT+TGG + chr5.4:71647017-71647036 None:intergenic 35.0%
AACACACCAATACTAACAGG+GGG + chr5.4:71646307-71646326 None:intergenic 40.0%
ACATGTCATAGAGTGAAGGA+TGG + chr5.4:71646958-71646977 None:intergenic 40.0%
ACCAAGCTAATGCAAGAACA+TGG - chr5.4:71646250-71646269 MS.gene038274:CDS 40.0%
AGAAGGTAATCCCATGAGAT+TGG + chr5.4:71647733-71647752 None:intergenic 40.0%
AGGCAACTTGGATGAAATGT+GGG - chr5.4:71646759-71646778 MS.gene038274:CDS 40.0%
AGTACATGCGACTTATTAGC+GGG + chr5.4:71647045-71647064 None:intergenic 40.0%
ATGCCTAGTCCTAAAAGGAA+TGG - chr5.4:71647631-71647650 MS.gene038274:CDS 40.0%
CCAAAATCTACCTTTGACTC+TGG + chr5.4:71647544-71647563 None:intergenic 40.0%
CCAAGCTAATGCAAGAACAT+GGG - chr5.4:71646251-71646270 MS.gene038274:CDS 40.0%
CTTCAACAAAATCCACACCA+CGG + chr5.4:71646489-71646508 None:intergenic 40.0%
GAAATGGTCACTAACTCCAT+GGG - chr5.4:71646439-71646458 MS.gene038274:CDS 40.0%
GAACACACCAATACTAACAG+GGG + chr5.4:71646308-71646327 None:intergenic 40.0%
GACACGGAAGAACATTTCTT+GGG - chr5.4:71647403-71647422 MS.gene038274:CDS 40.0%
GTATAATTGTGCAGGCAACT+TGG - chr5.4:71646747-71646766 MS.gene038274:CDS 40.0%
GTCCTAAAAGGAATGGTGAT+TGG - chr5.4:71647638-71647657 MS.gene038274:CDS 40.0%
GTTTCCTATTACGTCTTTGC+TGG - chr5.4:71646415-71646434 MS.gene038274:CDS 40.0%
TCTCAATTTGCATAAGCCAG+AGG - chr5.4:71646534-71646553 MS.gene038274:CDS 40.0%
TGAAATGGTCACTAACTCCA+TGG - chr5.4:71646438-71646457 MS.gene038274:CDS 40.0%
TGACATGTACATCTCCATCT+CGG - chr5.4:71646970-71646989 MS.gene038274:CDS 40.0%
TGTTGCAAAAATGGGGATTG+TGG - chr5.4:71647210-71647229 MS.gene038274:intron 40.0%
TTACGTCTTTGCTGGTGAAA+TGG - chr5.4:71646423-71646442 MS.gene038274:CDS 40.0%
TTCACCAGCAAAGACGTAAT+AGG + chr5.4:71646422-71646441 None:intergenic 40.0%
TTCTGCTTTCTTGTGGAAGA+TGG - chr5.4:71647153-71647172 MS.gene038274:CDS 40.0%
! AAAAGGGACCTCTGAAAACA+AGG + chr5.4:71646911-71646930 None:intergenic 40.0%
! AGCACACCATGTGTTTTCTT+TGG + chr5.4:71646583-71646602 None:intergenic 40.0%
! CAAAGGTAGATTTTGGATGG+GGG - chr5.4:71647548-71647567 MS.gene038274:CDS 40.0%
! CCAGAGTCAAAGGTAGATTT+TGG - chr5.4:71647541-71647560 MS.gene038274:CDS 40.0%
! GTGGTGCCAATAGTAATCAT+AGG + chr5.4:71646346-71646365 None:intergenic 40.0%
!! AAGACTTGTTATTGCTCGAG+CGG + chr5.4:71646871-71646890 None:intergenic 40.0%
!! CCCCACAATAGTGTTATTTG+TGG + chr5.4:71646365-71646384 None:intergenic 40.0%
!!! AGAGCGAGCTCATGATTTTT+TGG - chr5.4:71647372-71647391 MS.gene038274:CDS 40.0%
!!! CAACACAACCTTGTTTTCAG+AGG - chr5.4:71646900-71646919 MS.gene038274:CDS 40.0%
!!! TCACCATTCCTTTTAGGACT+AGG + chr5.4:71647637-71647656 None:intergenic 40.0%
!!! TCATGATTTTTTGGAGGCTG+CGG - chr5.4:71647381-71647400 MS.gene038274:CDS 40.0%
!!! TTCCTTTTAGGACTAGGCAT+TGG + chr5.4:71647631-71647650 None:intergenic 40.0%
AAAATGGGGATTGTGGTCGA+CGG - chr5.4:71647217-71647236 MS.gene038274:intron 45.0%
AAGCCAGAGGAAACCATTGA+AGG - chr5.4:71646547-71646566 MS.gene038274:CDS 45.0%
AGCCAGAGGAAACCATTGAA+GGG - chr5.4:71646548-71646567 MS.gene038274:CDS 45.0%
AGGATTTATTGCGGAGTTCC+TGG - chr5.4:71647472-71647491 MS.gene038274:CDS 45.0%
ATTTATTGCGGAGTTCCTGG+TGG - chr5.4:71647475-71647494 MS.gene038274:CDS 45.0%
CAACTTGGATGAAATGTGGG+GGG - chr5.4:71646762-71646781 MS.gene038274:CDS 45.0%
CAACTTTGACCATCGGATAG+CGG - chr5.4:71646799-71646818 MS.gene038274:CDS 45.0%
CAATAAATCCTTGCTAGCCC+TGG + chr5.4:71647463-71647482 None:intergenic 45.0%
CAGGCAACTTGGATGAAATG+TGG - chr5.4:71646758-71646777 MS.gene038274:CDS 45.0%
CCAGAAGACACCACAAATGT+CGG + chr5.4:71647511-71647530 None:intergenic 45.0%
CCCATGTTCTTGCATTAGCT+TGG + chr5.4:71646254-71646273 None:intergenic 45.0%
CGCTCTGTAACCCAACATAA+TGG + chr5.4:71647358-71647377 None:intergenic 45.0%
CTACTTCCCCCTGTTAGTAT+TGG - chr5.4:71646298-71646317 MS.gene038274:CDS 45.0%
CTTGGGACTAATTGATTGGG+TGG - chr5.4:71647420-71647439 MS.gene038274:CDS 45.0%
GCAACTTGGATGAAATGTGG+GGG - chr5.4:71646761-71646780 MS.gene038274:CDS 45.0%
GGAAACAAGAGCTTCAGCTA+AGG + chr5.4:71646401-71646420 None:intergenic 45.0%
GGAATACTTGCACTTCATGG+AGG - chr5.4:71647684-71647703 MS.gene038274:CDS 45.0%
GGACACGGAAGAACATTTCT+TGG - chr5.4:71647402-71647421 MS.gene038274:CDS 45.0%
GGCAACTTGGATGAAATGTG+GGG - chr5.4:71646760-71646779 MS.gene038274:CDS 45.0%
GTCATAGAGTGAAGGATGGA+TGG + chr5.4:71646954-71646973 None:intergenic 45.0%
TAGAGTGAAGGATGGATGGA+AGG + chr5.4:71646950-71646969 None:intergenic 45.0%
TGCCAATGCCTAGTCCTAAA+AGG - chr5.4:71647626-71647645 MS.gene038274:CDS 45.0%
TGGAACAAGCTTCCCTTCAA+TGG + chr5.4:71646563-71646582 None:intergenic 45.0%
TGGCATGCAACTTTGACCAT+CGG - chr5.4:71646792-71646811 MS.gene038274:CDS 45.0%
TTCCCTTCAATGGTTTCCTC+TGG + chr5.4:71646553-71646572 None:intergenic 45.0%
! GGGCTAGCAAGGATTTATTG+CGG - chr5.4:71647463-71647482 MS.gene038274:CDS 45.0%
!! AGACTTGTTATTGCTCGAGC+GGG + chr5.4:71646870-71646889 None:intergenic 45.0%
!! TGTGTTGGCTAGTAACTGCA+TGG + chr5.4:71647092-71647111 None:intergenic 45.0%
!!! GCGAGCTCATGATTTTTTGG+AGG - chr5.4:71647375-71647394 MS.gene038274:CDS 45.0%
AAGTAGCTCAGGTTCACCCA+TGG + chr5.4:71646458-71646477 None:intergenic 50.0%
ACTTTGCAACAACCGTGGTG+TGG - chr5.4:71646474-71646493 MS.gene038274:CDS 50.0%
AGCAGATGGACCGACATTTG+TGG - chr5.4:71647498-71647517 MS.gene038274:CDS 50.0%
AGCTGCAACTACCTTCTCGT+TGG + chr5.4:71646230-71646249 None:intergenic 50.0%
AGTGAAGGATGGATGGAAGG+TGG + chr5.4:71646947-71646966 None:intergenic 50.0%
CCGACATTTGTGGTGTCTTC+TGG - chr5.4:71647508-71647527 MS.gene038274:CDS 50.0%
CGGTTGTTGCAAAGTAGCTC+AGG + chr5.4:71646469-71646488 None:intergenic 50.0%
GTGAAGGATGGATGGAAGGT+GGG + chr5.4:71646946-71646965 None:intergenic 50.0%
TCAGCCGCTTCAACCAATAG+TGG - chr5.4:71647181-71647200 MS.gene038274:intron 50.0%
TGGGTGTCTTGGGGTCAAAA+GGG + chr5.4:71646927-71646946 None:intergenic 50.0%
! ATTCCTTGTGGTGGTGATGC+TGG - chr5.4:71647595-71647614 MS.gene038274:CDS 50.0%
! GAAATGTGGGGGGTTAGTAG+TGG - chr5.4:71646772-71646791 MS.gene038274:CDS 50.0%
! GAGCTACTTTGCAACAACCG+TGG - chr5.4:71646469-71646488 MS.gene038274:CDS 50.0%
!! ACATGGTGTGCTTGCTGTTC+AGG - chr5.4:71646591-71646610 MS.gene038274:CDS 50.0%
!!! TTTTTTGGAGGCTGCGGACA+CGG - chr5.4:71647387-71647406 MS.gene038274:CDS 50.0%
AATAGGCGTCCGCTATCCGA+TGG + chr5.4:71646811-71646830 None:intergenic 55.0%
ATCCTTGCTAGCCCTGGAAC+CGG + chr5.4:71647457-71647476 None:intergenic 55.0%
GATGGAAGGTGGGTGTCTTG+GGG + chr5.4:71646936-71646955 None:intergenic 55.0%
GGATGGAAGGTGGGTGTCTT+GGG + chr5.4:71646937-71646956 None:intergenic 55.0%
GTGGGTGTCTTGGGGTCAAA+AGG + chr5.4:71646928-71646947 None:intergenic 55.0%
TAGCCAGCATCACCACCACA+AGG + chr5.4:71647601-71647620 None:intergenic 55.0%
TGGAGGAGGTAGATCCGAGA+TGG + chr5.4:71646987-71647006 None:intergenic 55.0%
TGGATGGAAGGTGGGTGTCT+TGG + chr5.4:71646938-71646957 None:intergenic 55.0%
!! AAGAGCATCGACCGGTTCCA+GGG - chr5.4:71647443-71647462 MS.gene038274:CDS 55.0%
!! ATTGCTCGAGCGGGCTATCT+CGG + chr5.4:71646861-71646880 None:intergenic 55.0%
!! TCTGATTCGGGCTTGGGTTC+AGG + chr5.4:71647010-71647029 None:intergenic 55.0%
!! TTGGGTGGAAGAGCATCGAC+CGG - chr5.4:71647435-71647454 MS.gene038274:CDS 55.0%
GATTCGGGCTTGGGTTCAGG+TGG + chr5.4:71647007-71647026 None:intergenic 60.0%
GGAGTTCCTGGTGGAGCAGA+TGG - chr5.4:71647484-71647503 MS.gene038274:CDS 60.0%
GGATGGGGGAAACCCGTAGT+TGG - chr5.4:71647562-71647581 MS.gene038274:CDS 60.0%
!! GAAGAGCATCGACCGGTTCC+AGG - chr5.4:71647442-71647461 MS.gene038274:CDS 60.0%
GGCTTGGGTTCAGGTGGAGG+AGG + chr5.4:71647001-71647020 None:intergenic 65.0%
GTCGGTCCATCTGCTCCACC+AGG + chr5.4:71647493-71647512 None:intergenic 65.0%
TCGGGCTTGGGTTCAGGTGG+AGG + chr5.4:71647004-71647023 None:intergenic 65.0%
! GACCGGTTCCAGGGCTAGCA+AGG - chr5.4:71647452-71647471 MS.gene038274:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 71646190 71647759 71646190 ID=MS.gene038274
chr5.4 mRNA 71646190 71647759 71646190 ID=MS.gene038274.t1;Parent=MS.gene038274
chr5.4 exon 71647337 71647759 71647337 ID=MS.gene038274.t1.exon1;Parent=MS.gene038274.t1
chr5.4 CDS 71647337 71647759 71647337 ID=cds.MS.gene038274.t1;Parent=MS.gene038274.t1
chr5.4 exon 71646190 71647188 71646190 ID=MS.gene038274.t1.exon2;Parent=MS.gene038274.t1
chr5.4 CDS 71646190 71647188 71646190 ID=cds.MS.gene038274.t1;Parent=MS.gene038274.t1
Gene Sequence

>MS.gene038274

ATGAGTATTGGAAAAGTAGAATTCAGTGTCAATATTACCAACGAGAAGGTAGTTGCAGCTACCAAGCTAATGCAAGAACATGGGCTAGCACTATCAAATCTTGATTTACTACTTCCCCCTGTTAGTATTGGTGTGTTCTTTTGCTACAACAATCCTATGATTACTATTGGCACCACAAATAACACTATTGTGGGGTGTTTGAAAAATTCCTTAGCTGAAGCTCTTGTTTCCTATTACGTCTTTGCTGGTGAAATGGTCACTAACTCCATGGGTGAACCTGAGCTACTTTGCAACAACCGTGGTGTGGATTTTGTTGAAGCTGTTGTTGATGTTGAGTTACAATCTCTCAATTTGCATAAGCCAGAGGAAACCATTGAAGGGAAGCTTGTTCCAAAGAAAACACATGGTGTGCTTGCTGTTCAGGTGAAAGTTTTTAATATTTGTTCATATTTACAAAAACATTTTGATCTCAAGGGAGTATTAGAATAGTGAAAGAATTATTATCATTATGCAGGTTTTTAGTTAATGATTAGTAGTAAGAAGTGTTTCAATTGTATGTATAATTGTGCAGGCAACTTGGATGAAATGTGGGGGGTTAGTAGTGGCATGCAACTTTGACCATCGGATAGCGGACGCCTATTCTACAAACATGTTTCTTGTATCATGGGCCGAGATAGCCCGCTCGAGCAATAACAAGTCTTTGATCTCAACAACACAACCTTGTTTTCAGAGGTCCCTTTTGACCCCAAGACACCCACCTTCCATCCATCCTTCACTCTATGACATGTACATCTCCATCTCGGATCTACCTCCTCCACCTGAACCCAAGCCCGAATCAGATATTAAAACAAACCCGCTAATAAGTCGCATGTACTACGTAACAAGCGAAGAACTCAACACCATGCAGTTACTAGCCAACACAAATAATAGTGGAAGCATTAAGCGCTCAAAACTTGAATCATTTTCTGCTTTCTTGTGGAAGATGGTTGCATCAGCCGCTTCAACCAATAGTGGAAATGTTGTTGCAAAAATGGGGATTGTGGTCGACGGAAGAATGAGATTAAGCAATGGTGACAAAAACAAAGAAGAGTTTATGAGTTCGTATTTTGGGAATGTGCTTTCAACTCCCTACAGTTGTAAATTAATAGAAGAACTTGTTGAAAAGCCATTATGTTGGGTTACAGAGCGAGCTCATGATTTTTTGGAGGCTGCGGACACGGAAGAACATTTCTTGGGACTAATTGATTGGGTGGAAGAGCATCGACCGGTTCCAGGGCTAGCAAGGATTTATTGCGGAGTTCCTGGTGGAGCAGATGGACCGACATTTGTGGTGTCTTCTGGACAAAGATTTCCAGAGTCAAAGGTAGATTTTGGATGGGGGAAACCCGTAGTTGGATCTTATCATATTCCTTGTGGTGGTGATGCTGGCTATGTTATGCCAATGCCTAGTCCTAAAAGGAATGGTGATTGGTTGATCTACATGAATCTTCCTAAGGAATACTTGCACTTCATGGAGGCTCAAGCTCCCCATTTCTTTAGACCAATCTCATGGGATTACCTTCTCAATTAA
Protein sequence

>MS.gene038274.t1

MSIGKVEFSVNITNEKVVAATKLMQEHGLALSNLDLLLPPVSIGVFFCYNNPMITIGTTNNTIVGCLKNSLAEALVSYYVFAGEMVTNSMGEPELLCNNRGVDFVEAVVDVELQSLNLHKPEETIEGKLVPKKTHGVLAVQATWMKCGGLVVACNFDHRIADAYSTNMFLVSWAEIARSSNNKSLISTTQPCFQRSLLTPRHPPSIHPSLYDMYISISDLPPPPEPKPESDIKTNPLISRMYYVTSEELNTMQLLANTNNSGSIKRSKLESFSAFLWKMVASAASTNSGNVVAKMGIVVDGRMRLSNGDKNKEEFMSSYFGNVLSTPYSCKLIEELVEKPLCWVTERAHDFLEAADTEEHFLGLIDWVEEHRPVPGLARIYCGVPGGADGPTFVVSSGQRFPESKVDFGWGKPVVGSYHIPCGGDAGYVMPMPSPKRNGDWLIYMNLPKEYLHFMEAQAPHFFRPISWDYLLN