Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030751.t1 | EFH50611.1 | 100 | 93 | 0 | 0 | 1 | 93 | 1 | 93 | 9.40E-49 | 203 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030751.t1 | F4K4E3 | 98.9 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 5.0e-51 | 201.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030751.t1 | G7KRW1 | 100.0 | 93 | 0 | 0 | 1 | 93 | 1 | 93 | 6.8e-49 | 203.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43700 | MS.gene030751 | PPI |
| MS.gene051241 | MS.gene030751 | PPI |
| MS.gene030751 | MS.gene023131 | PPI |
| MS.gene36469 | MS.gene030751 | PPI |
| MS.gene051172 | MS.gene030751 | PPI |
| MS.gene35136 | MS.gene030751 | PPI |
| MS.gene051245 | MS.gene030751 | PPI |
| MS.gene028322 | MS.gene030751 | PPI |
| MS.gene030751 | MS.gene006962 | PPI |
| MS.gene055162 | MS.gene030751 | PPI |
| MS.gene23567 | MS.gene030751 | PPI |
| MS.gene44813 | MS.gene030751 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030751.t1 | MTR_8g058537 | 100.000 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 5.05e-103 | 291 |
| MS.gene030751.t1 | MTR_8g058420 | 100.000 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 5.05e-103 | 291 |
| MS.gene030751.t1 | MTR_7g108700 | 100.000 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 7.37e-103 | 290 |
| MS.gene030751.t1 | MTR_8g091620 | 98.601 | 143 | 2 | 0 | 1 | 143 | 1 | 143 | 4.67e-101 | 286 |
| MS.gene030751.t1 | MTR_1g088710 | 73.267 | 101 | 9 | 1 | 1 | 101 | 1 | 83 | 1.13e-49 | 154 |
| MS.gene030751.t1 | MTR_8g092200 | 32.231 | 121 | 78 | 2 | 2 | 122 | 7 | 123 | 3.62e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030751.t1 | AT5G27720 | 77.536 | 138 | 10 | 1 | 1 | 138 | 1 | 117 | 1.44e-60 | 183 |
| MS.gene030751.t1 | AT1G76300 | 33.333 | 99 | 65 | 1 | 2 | 100 | 7 | 104 | 1.18e-14 | 66.2 |
| MS.gene030751.t1 | AT1G20580 | 33.663 | 101 | 64 | 2 | 2 | 100 | 7 | 106 | 3.85e-14 | 65.1 |
Find 0 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAGTAAAATAAATAAAA+AGG | - | 57601:13985-14004 | None:intergenic | 10.0% |
| !!! | AACTTTATTTTTTATTTAAC+TGG | - | 57601:15292-15311 | None:intergenic | 10.0% |
| !!! | ATTTTCTTAAATAACATAAA+AGG | - | 57601:14575-14594 | None:intergenic | 10.0% |
| !!! | TTTTCTTAAATAACATAAAA+GGG | - | 57601:14574-14593 | None:intergenic | 10.0% |
| !!! | TTTTTATTTATTTTACTTTG+TGG | + | 57601:13984-14003 | MS.gene030751:intron | 10.0% |
| !! | AATCAGAAGAAAATTATATA+TGG | - | 57601:15169-15188 | None:intergenic | 15.0% |
| !!! | ATGTTATTTAAGAAAATCAT+TGG | + | 57601:14578-14597 | MS.gene030751:intron | 15.0% |
| !!! | TTTAATTTGTTATTGTTGAA+AGG | + | 57601:14209-14228 | MS.gene030751:intron | 15.0% |
| !! | AAATTATCTGATTCACTAAA+AGG | - | 57601:14741-14760 | None:intergenic | 20.0% |
| !! | AATTATCTGATTCACTAAAA+GGG | - | 57601:14740-14759 | None:intergenic | 20.0% |
| !! | AGTTATTTATAAAGAGATCA+AGG | + | 57601:14376-14395 | MS.gene030751:intron | 20.0% |
| !! | GTTATTTATAAAGAGATCAA+GGG | + | 57601:14377-14396 | MS.gene030751:intron | 20.0% |
| !! | TATTTCAATCCAAAATCTAA+AGG | - | 57601:15003-15022 | None:intergenic | 20.0% |
| !! | TTTAAACTAATGATATGAAG+TGG | - | 57601:14456-14475 | None:intergenic | 20.0% |
| !!! | TATGAATTTTGATTGAAACT+GGG | + | 57601:14238-14257 | MS.gene030751:intron | 20.0% |
| !!! | TTAGATTTTGGATTGAAATA+TGG | + | 57601:15003-15022 | MS.gene030751:intron | 20.0% |
| !!! | TTATGAATTTTGATTGAAAC+TGG | + | 57601:14237-14256 | MS.gene030751:intron | 20.0% |
| ! | AAAAATGGAGAGACATATAA+TGG | + | 57601:14084-14103 | MS.gene030751:CDS | 25.0% |
| ! | AAAATGGAGAGACATATAAT+GGG | + | 57601:14085-14104 | MS.gene030751:CDS | 25.0% |
| ! | TAAGAAAATCATTGGTGAAT+AGG | + | 57601:14586-14605 | MS.gene030751:intron | 25.0% |
| ! | TGATAAATCCTAAATTGACT+TGG | + | 57601:14927-14946 | MS.gene030751:intron | 25.0% |
| ! | TTAACAAAATTGAACCACTA+GGG | + | 57601:13828-13847 | MS.gene030751:intron | 25.0% |
| ! | TTACCTTGATACATGAATAT+TGG | + | 57601:15122-15141 | MS.gene030751:intron | 25.0% |
| ! | TTTAACAAAATTGAACCACT+AGG | + | 57601:13827-13846 | MS.gene030751:intron | 25.0% |
| !!! | AAAAAAGAGCATTACTTTAG+AGG | - | 57601:14166-14185 | None:intergenic | 25.0% |
| AAGCTGAGTAAGATATAAAG+TGG | + | 57601:14957-14976 | MS.gene030751:intron | 30.0% | |
| AATAATACTTCGTACCTGTA+CGG | - | 57601:14856-14875 | None:intergenic | 30.0% | |
| ACACCAATATTCATGTATCA+AGG | - | 57601:15128-15147 | None:intergenic | 30.0% | |
| AGATTGATATGTTGGAATAG+TGG | + | 57601:14500-14519 | MS.gene030751:intron | 30.0% | |
| ATTTGGTTAATTGCGATACA+TGG | + | 57601:14109-14128 | MS.gene030751:CDS | 30.0% | |
| GCAATAGTAGATTGATATGT+TGG | + | 57601:14492-14511 | MS.gene030751:intron | 30.0% | |
| GTAACTACTTTACCTTTCAA+AGG | + | 57601:13763-13782 | MS.gene030751:intron | 30.0% | |
| TTGTCGATAACCTAAATCAA+AGG | - | 57601:14815-14834 | None:intergenic | 30.0% | |
| ! | AATCTGTCACCTTTAGATTT+TGG | + | 57601:14991-15010 | MS.gene030751:intron | 30.0% |
| ! | TAGAGGTACAAATAACTTCT+CGG | - | 57601:14149-14168 | None:intergenic | 30.0% |
| ! | TGCTTTATTGCCTTTGATTT+AGG | + | 57601:14802-14821 | MS.gene030751:intron | 30.0% |
| ! | TTCTGATTGATTCTGACTTA+GGG | + | 57601:15181-15200 | MS.gene030751:intron | 30.0% |
| !! | AATCCTAAATTGACTTGGAA+TGG | + | 57601:14932-14951 | MS.gene030751:intron | 30.0% |
| !! | AGGTTTTTGTTACTTACCAT+AGG | - | 57601:13965-13984 | None:intergenic | 30.0% |
| !! | ATCCTAAATTGACTTGGAAT+GGG | + | 57601:14933-14952 | MS.gene030751:intron | 30.0% |
| !! | ATTTGTTATTGTTGAAAGGC+TGG | + | 57601:14213-14232 | MS.gene030751:intron | 30.0% |
| !! | GGTTTTTGTTACTTACCATA+GGG | - | 57601:13964-13983 | None:intergenic | 30.0% |
| !! | TTGGAATAGTGGATTCTTAT+TGG | + | 57601:14511-14530 | MS.gene030751:intron | 30.0% |
| !! | TTTGAACTTGTTGTAACAGT+TGG | + | 57601:14053-14072 | MS.gene030751:intron | 30.0% |
| AAATGGCATTTCAACTTGTG+TGG | - | 57601:15258-15277 | None:intergenic | 35.0% | |
| AACCTTACCTTGTGATCTAT+TGG | + | 57601:14623-14642 | MS.gene030751:intron | 35.0% | |
| AATGGCATTTCAACTTGTGT+GGG | - | 57601:15257-15276 | None:intergenic | 35.0% | |
| GACTATAACATAAACCCTAG+TGG | - | 57601:13845-13864 | None:intergenic | 35.0% | |
| TATTGGTCATGGGTTTAGTT+GGG | + | 57601:14640-14659 | MS.gene030751:intron | 35.0% | |
| TTAGGTTATCGACAAAGTTC+AGG | + | 57601:14820-14839 | MS.gene030751:intron | 35.0% | |
| TTGATTGAAACTGGGAATGT+AGG | + | 57601:14246-14265 | MS.gene030751:intron | 35.0% | |
| ! | ACACTACAGAGATGTAGTTT+TGG | - | 57601:15040-15059 | None:intergenic | 35.0% |
| ! | ACTACATCTCTGTAGTGTTT+TGG | + | 57601:15042-15061 | MS.gene030751:intron | 35.0% |
| ! | CTTCTGATTGATTCTGACTT+AGG | + | 57601:15180-15199 | MS.gene030751:intron | 35.0% |
| ! | TGTAGGATGGAGATAGATTT+TGG | + | 57601:14263-14282 | MS.gene030751:intron | 35.0% |
| !! | AGAGACATATAATGGGCATT+TGG | + | 57601:14092-14111 | MS.gene030751:CDS | 35.0% |
| !! | ATTCACTGTTAGCCTTTGAA+AGG | - | 57601:13778-13797 | None:intergenic | 35.0% |
| !! | TTTTGTTACTTACCATAGGG+TGG | - | 57601:13961-13980 | None:intergenic | 35.0% |
| AACTGGACGACTGCATAAAA+TGG | - | 57601:15275-15294 | None:intergenic | 40.0% | |
| ACCCAACAAACTTTCTCTGT+TGG | + | 57601:15083-15102 | MS.gene030751:intron | 40.0% | |
| ACCCAACAGAGAAAGTTTGT+TGG | - | 57601:15088-15107 | None:intergenic | 40.0% | |
| ACCTTGTGATCTATTGGTCA+TGG | + | 57601:14629-14648 | MS.gene030751:intron | 40.0% | |
| CCCAACAAACTTTCTCTGTT+GGG | + | 57601:15084-15103 | MS.gene030751:intron | 40.0% | |
| CCTTGTGATCTATTGGTCAT+GGG | + | 57601:14630-14649 | MS.gene030751:intron | 40.0% | |
| CTATTGGTCATGGGTTTAGT+TGG | + | 57601:14639-14658 | MS.gene030751:intron | 40.0% | |
| GACCAATAGATCACAAGGTA+AGG | - | 57601:14628-14647 | None:intergenic | 40.0% | |
| GCAGTCTTAAGAAGAGAAAG+AGG | - | 57601:13932-13951 | None:intergenic | 40.0% | |
| GTTCCTGATGAGGTATGTAA+TGG | + | 57601:14334-14353 | MS.gene030751:intron | 40.0% | |
| TCTAAAGGTGACAGATTCAC+AGG | - | 57601:14988-15007 | None:intergenic | 40.0% | |
| TCTCTTCTTAAGACTGCACA+AGG | + | 57601:13935-13954 | MS.gene030751:CDS | 40.0% | |
| TGCCCATTCCAAGTCAATTT+AGG | - | 57601:14938-14957 | None:intergenic | 40.0% | |
| TGGTGAATAGGTTGCTAAGA+AGG | + | 57601:14598-14617 | MS.gene030751:intron | 40.0% | |
| TTGAAACTGGGAATGTAGGA+TGG | + | 57601:14250-14269 | MS.gene030751:intron | 40.0% | |
| TTTCCTCGTATGTAGCATTC+AGG | - | 57601:14295-14314 | None:intergenic | 40.0% | |
| ! | AGGATGGAGATAGATTTTGG+CGG | + | 57601:14266-14285 | MS.gene030751:intron | 40.0% |
| ! | ATTTCTGATTCGTCGCAACA+TGG | - | 57601:13802-13821 | None:intergenic | 40.0% |
| ! | CCCAACAGAGAAAGTTTGTT+GGG | - | 57601:15087-15106 | None:intergenic | 40.0% |
| !! | GAACTTGTTGTAACAGTTGG+TGG | + | 57601:14056-14075 | MS.gene030751:intron | 40.0% |
| !!! | CTACAGAGATGTAGTTTTGG+TGG | - | 57601:15037-15056 | None:intergenic | 40.0% |
| AGAGTATGAGCACAACCTTG+AGG | - | 57601:14692-14711 | None:intergenic | 45.0% | |
| ATACCTCATCAGGAACTCGA+AGG | - | 57601:14330-14349 | None:intergenic | 45.0% | |
| ATGCCTGAATGCTACATACG+AGG | + | 57601:14289-14308 | MS.gene030751:CDS | 45.0% | |
| CCACCATTACATACCTCATC+AGG | - | 57601:14340-14359 | None:intergenic | 45.0% | |
| CCCATGACCAATAGATCACA+AGG | - | 57601:14633-14652 | None:intergenic | 45.0% | |
| CCTGATGAGGTATGTAATGG+TGG | + | 57601:14337-14356 | MS.gene030751:intron | 45.0% | |
| GAGGAAGCTGCAACAAAAGA+AGG | - | 57601:13913-13932 | None:intergenic | 45.0% | |
| GGGAAAAACCTTGTGCTTGA+GGG | + | 57601:14660-14679 | MS.gene030751:intron | 45.0% | |
| TGGGAAAAACCTTGTGCTTG+AGG | + | 57601:14659-14678 | MS.gene030751:intron | 45.0% | |
| !! | CAGTTGGTGGAGCTGAAAAA+TGG | + | 57601:14069-14088 | MS.gene030751:intron | 45.0% |
| CAGGAAGAAAAGAGCCGTAC+AGG | + | 57601:14839-14858 | MS.gene030751:CDS | 50.0% | |
| GCTTGAGGGTTTAAGCCTCA+AGG | + | 57601:14674-14693 | MS.gene030751:intron | 50.0% | |
| GGCTTAAACCCTCAAGCACA+AGG | - | 57601:14671-14690 | None:intergenic | 50.0% | |
| ! | GGTGGCTTGCGATCTGAAAA+AGG | - | 57601:15330-15349 | None:intergenic | 50.0% |
| ! | GTACCTTCGAGTTCCTGATG+AGG | + | 57601:14324-14343 | MS.gene030751:CDS | 50.0% |
| ! | TTTTCAGATCGCAAGCCACC+TGG | + | 57601:15330-15349 | MS.gene030751:intron | 50.0% |
| !! | GACGCCAAACAAAAGGCACT+GGG | + | 57601:15403-15422 | MS.gene030751:CDS | 50.0% |
| !! | TTTGATGATGGTGGGGCTAG+AGG | + | 57601:15432-15451 | MS.gene030751:CDS | 50.0% |
| !!! | ACTGGGCGTGGTTTTGATGA+TGG | + | 57601:15420-15439 | MS.gene030751:CDS | 50.0% |
| !!! | GCGTGGTTTTGATGATGGTG+GGG | + | 57601:15425-15444 | MS.gene030751:CDS | 50.0% |
| !!! | GGCGTGGTTTTGATGATGGT+GGG | + | 57601:15424-15443 | MS.gene030751:CDS | 50.0% |
| ACGTGGTAGGGGTAGAGGTA+GGG | + | 57601:15359-15378 | MS.gene030751:CDS | 55.0% | |
| TTCCTCCAGGCTTTCCACCT+TGG | - | 57601:15482-15501 | None:intergenic | 55.0% | |
| ! | CACGCCCAGTGCCTTTTGTT+TGG | - | 57601:15410-15429 | None:intergenic | 55.0% |
| ! | GCTGGTGGACGCCAAACAAA+AGG | + | 57601:15396-15415 | MS.gene030751:CDS | 55.0% |
| ! | GGACGCCAAACAAAAGGCAC+TGG | + | 57601:15402-15421 | MS.gene030751:CDS | 55.0% |
| !! | CAAACAAAAGGCACTGGGCG+TGG | + | 57601:15408-15427 | MS.gene030751:CDS | 55.0% |
| !!! | GGGCGTGGTTTTGATGATGG+TGG | + | 57601:15423-15442 | MS.gene030751:CDS | 55.0% |
| ACCTGGTGTGGGACGTGGTA+GGG | + | 57601:15347-15366 | MS.gene030751:CDS | 60.0% | |
| AGAGGTAGGGAGGATGCTGC+TGG | + | 57601:15372-15391 | MS.gene030751:CDS | 60.0% | |
| AGATCGCAAGCCACCTGGTG+TGG | + | 57601:15335-15354 | MS.gene030751:intron | 60.0% | |
| AGCCAAGGTGGAAAGCCTGG+AGG | + | 57601:15477-15496 | MS.gene030751:CDS | 60.0% | |
| GACGTGGTAGGGGTAGAGGT+AGG | + | 57601:15358-15377 | MS.gene030751:CDS | 60.0% | |
| GACTGCACAAGGCCACCCTA+TGG | + | 57601:13946-13965 | MS.gene030751:CDS | 60.0% | |
| GATCGCAAGCCACCTGGTGT+GGG | + | 57601:15336-15355 | MS.gene030751:intron | 60.0% | |
| GCTAGCCAAGGTGGAAAGCC+TGG | + | 57601:15474-15493 | MS.gene030751:CDS | 60.0% | |
| TGGTAGGGGTAGAGGTAGGG+AGG | + | 57601:15362-15381 | MS.gene030751:CDS | 60.0% | |
| AAGCCACCTGGTGTGGGACG+TGG | + | 57601:15342-15361 | MS.gene030751:CDS | 65.0% | |
| AGGGAGGATGCTGCTGGTGC+TGG | + | 57601:15378-15397 | MS.gene030751:CDS | 65.0% | |
| CACCTGGTGTGGGACGTGGT+AGG | + | 57601:15346-15365 | MS.gene030751:CDS | 65.0% | |
| CCTGGTGTGGGACGTGGTAG+GGG | + | 57601:15348-15367 | MS.gene030751:CDS | 65.0% | |
| CTACCACGTCCCACACCAGG+TGG | - | 57601:15348-15367 | None:intergenic | 65.0% | |
| GTGGGACGTGGTAGGGGTAG+AGG | + | 57601:15354-15373 | MS.gene030751:CDS | 65.0% | |
| ! | CACCTTGGCTAGCCCTGCCT+CGG | - | 57601:15467-15486 | None:intergenic | 65.0% |
| ! | GAGGATGCTGCTGGTGCTGG+TGG | + | 57601:15381-15400 | MS.gene030751:CDS | 65.0% |
| !! | GATGGTGGGGCTAGAGGAGC+CGG | + | 57601:15438-15457 | MS.gene030751:CDS | 65.0% |
| CCCCTACCACGTCCCACACC+AGG | - | 57601:15351-15370 | None:intergenic | 70.0% | |
| !! | CGAGGCAGGGCTAGCCAAGG+TGG | + | 57601:15465-15484 | MS.gene030751:CDS | 70.0% |
| AGGAGCCGGAGGCCGAGGCA+GGG | + | 57601:15452-15471 | MS.gene030751:CDS | 75.0% | |
| GCTAGAGGAGCCGGAGGCCG+AGG | + | 57601:15447-15466 | MS.gene030751:CDS | 75.0% | |
| GCTAGCCCTGCCTCGGCCTC+CGG | - | 57601:15460-15479 | None:intergenic | 75.0% | |
| GGTGGGGCTAGAGGAGCCGG+AGG | + | 57601:15441-15460 | MS.gene030751:CDS | 75.0% | |
| !! | GGCCGAGGCAGGGCTAGCCA+AGG | + | 57601:15462-15481 | MS.gene030751:CDS | 75.0% |
| ! | GAGGAGCCGGAGGCCGAGGC+AGG | + | 57601:15451-15470 | MS.gene030751:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57601 | gene | 13760 | 15507 | 13760 | ID=MS.gene030751 |
| 57601 | mRNA | 13760 | 15507 | 13760 | ID=MS.gene030751.t1;Parent=MS.gene030751 |
| 57601 | exon | 13760 | 13762 | 13760 | ID=MS.gene030751.t1.exon1;Parent=MS.gene030751.t1 |
| 57601 | CDS | 13760 | 13762 | 13760 | ID=cds.MS.gene030751.t1;Parent=MS.gene030751.t1 |
| 57601 | exon | 13926 | 13967 | 13926 | ID=MS.gene030751.t1.exon2;Parent=MS.gene030751.t1 |
| 57601 | CDS | 13926 | 13967 | 13926 | ID=cds.MS.gene030751.t1;Parent=MS.gene030751.t1 |
| 57601 | exon | 14072 | 14170 | 14072 | ID=MS.gene030751.t1.exon3;Parent=MS.gene030751.t1 |
| 57601 | CDS | 14072 | 14170 | 14072 | ID=cds.MS.gene030751.t1;Parent=MS.gene030751.t1 |
| 57601 | exon | 14268 | 14345 | 14268 | ID=MS.gene030751.t1.exon4;Parent=MS.gene030751.t1 |
| 57601 | CDS | 14268 | 14345 | 14268 | ID=cds.MS.gene030751.t1;Parent=MS.gene030751.t1 |
| 57601 | exon | 14824 | 14860 | 14824 | ID=MS.gene030751.t1.exon5;Parent=MS.gene030751.t1 |
| 57601 | CDS | 14824 | 14860 | 14824 | ID=cds.MS.gene030751.t1;Parent=MS.gene030751.t1 |
| 57601 | exon | 15337 | 15507 | 15337 | ID=MS.gene030751.t1.exon6;Parent=MS.gene030751.t1 |
| 57601 | CDS | 15337 | 15507 | 15337 | ID=cds.MS.gene030751.t1;Parent=MS.gene030751.t1 |
| Gene Sequence |
| Protein sequence |
