AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35136

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35136.t1 MTR_4g094600 100.000 87 0 0 1 87 1 87 1.21e-59 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35136.t1 AT5G48870 95.294 85 4 0 1 85 1 85 1.46e-55 166

Find 21 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAGCAGCATTGTTCATTC+TGG 0.260713 4.4:-19537288 None:intergenic
CATAAACATCAAAGCCTCTA+AGG 0.282210 4.4:-19535434 None:intergenic
GAGCTTGTTGGTACCCTTAG+AGG 0.311837 4.4:+19535420 MS.gene35136:CDS
CCATCACAGCTTCTTCCATC+AGG 0.419048 4.4:+19535246 MS.gene35136:CDS
ACTATTCAGTAACATCTTCA+AGG 0.420043 4.4:-19535467 None:intergenic
AGCATTGTTCATTCTGGATC+AGG 0.435725 4.4:-19537282 None:intergenic
GAGCTGATTGACCGCTGTAT+AGG 0.443634 4.4:+19535363 MS.gene35136:intron
CTTTGATGTTTATGTTAACA+TGG 0.481181 4.4:+19535443 MS.gene35136:CDS
TGTGCAATGCAGTTGGTCCC+TGG 0.507142 4.4:+19537252 MS.gene35136:intron
AGATCACTGCTGAGGGGAGA+AGG 0.509229 4.4:+19537027 MS.gene35136:CDS
AAAGGCGACAAGGAGCTTGT+TGG 0.513402 4.4:+19535408 MS.gene35136:CDS
AAAATATGGGTGATAATGAA+AGG 0.540065 4.4:+19535390 MS.gene35136:CDS
CCTGATGGAAGAAGCTGTGA+TGG 0.541063 4.4:-19535246 None:intergenic
ATAAACATCAAAGCCTCTAA+GGG 0.563111 4.4:-19535433 None:intergenic
TCTGGATCAGGTGAACCACC+AGG 0.582965 4.4:-19537270 None:intergenic
AACAGCGAGATCACTGCTGA+GGG 0.596820 4.4:+19537020 MS.gene35136:intron
CAACAGCGAGATCACTGCTG+AGG 0.645210 4.4:+19537019 MS.gene35136:intron
GGTGATAATGAAAGGCGACA+AGG 0.648287 4.4:+19535398 MS.gene35136:CDS
GCAATGCAGTTGGTCCCTGG+TGG 0.679569 4.4:+19537255 MS.gene35136:intron
CTGGATCAGGTGAACCACCA+GGG 0.706934 4.4:-19537269 None:intergenic
ACAGCGAGATCACTGCTGAG+GGG 0.753357 4.4:+19537021 MS.gene35136:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATATACCATAGAATTT+AGG - chr4.4:19537134-19537153 None:intergenic 15.0%
!!! AAATGCATTTTAAAAATTTG+AGG - chr4.4:19535678-19535697 None:intergenic 15.0%
!!! AATGCATTTTAAAAATTTGA+GGG - chr4.4:19535677-19535696 None:intergenic 15.0%
!! ACAAAAGTTCAAATTAAGAT+AGG - chr4.4:19535982-19536001 None:intergenic 20.0%
!! ATAAACTAAATCAATTTGTG+AGG - chr4.4:19536983-19537002 None:intergenic 20.0%
!! ATGTAAACATTCATAAAAGT+TGG - chr4.4:19535752-19535771 None:intergenic 20.0%
!! TATTAATAAATCATTTGACC+TGG + chr4.4:19537147-19537166 MS.gene35136:intron 20.0%
!! TGTAAACATTCATAAAAGTT+GGG - chr4.4:19535751-19535770 None:intergenic 20.0%
!! TTAAGATTTAAGAAAGTGAA+GGG + chr4.4:19535929-19535948 MS.gene35136:intron 20.0%
!! TTCTATTTACCTAAATTCTA+TGG + chr4.4:19537122-19537141 MS.gene35136:intron 20.0%
!! TTTCAAATTTCTATGTTTCT+TGG + chr4.4:19536486-19536505 MS.gene35136:intron 20.0%
!!! TGTTTACATTTAATTTGAAC+TGG + chr4.4:19535763-19535782 MS.gene35136:intron 20.0%
!!! TTTTAAAATGCATTTCTAAC+TGG + chr4.4:19535683-19535702 MS.gene35136:intron 20.0%
! AGACTTTCTTACCTTTATAA+TGG + chr4.4:19536877-19536896 MS.gene35136:intron 25.0%
! AGTTTAAAAGATGACAATGT+CGG - chr4.4:19536566-19536585 None:intergenic 25.0%
! ATTAACTTAGTACATGAAAG+AGG - chr4.4:19536717-19536736 None:intergenic 25.0%
! CTGTATAGGTTCAAAAATAT+GGG + chr4.4:19535377-19535396 MS.gene35136:CDS 25.0%
! CTTTGATGTTTATGTTAACA+TGG + chr4.4:19535443-19535462 MS.gene35136:CDS 25.0%
! GGGAAATGAAAGTAAATATA+TGG - chr4.4:19536153-19536172 None:intergenic 25.0%
! GTAAACATTCATAAAAGTTG+GGG - chr4.4:19535750-19535769 None:intergenic 25.0%
! GTTAAGATTTAAGAAAGTGA+AGG + chr4.4:19535928-19535947 MS.gene35136:intron 25.0%
! TTGTTGGCACAAAAAAATAT+TGG + chr4.4:19535875-19535894 MS.gene35136:intron 25.0%
!! AAAATATGGGTGATAATGAA+AGG + chr4.4:19535390-19535409 MS.gene35136:CDS 25.0%
!! AATTTTTCCTTTATGCATTG+AGG - chr4.4:19536283-19536302 None:intergenic 25.0%
!! ACCAACTATGTTTTTCTAAT+TGG + chr4.4:19536654-19536673 MS.gene35136:intron 25.0%
!! AGTTTAGGTTTAGTTTATCA+TGG + chr4.4:19536620-19536639 MS.gene35136:intron 25.0%
!! CCAACTATGTTTTTCTAATT+GGG + chr4.4:19536655-19536674 MS.gene35136:intron 25.0%
!! CTTGATCAAATTTTACTCAA+TGG + chr4.4:19537059-19537078 MS.gene35136:CDS 25.0%
!! TGAGAATTTGAAACTTACAA+TGG - chr4.4:19537096-19537115 None:intergenic 25.0%
!! TTGGATAAAAGCATAAAATG+TGG - chr4.4:19535506-19535525 None:intergenic 25.0%
!!! ATATTTTTGAACCTATACAG+CGG - chr4.4:19535377-19535396 None:intergenic 25.0%
!!! GTTTGAAATTCAAGTTGTTT+GGG + chr4.4:19535897-19535916 MS.gene35136:intron 25.0%
AAACAGAGTTCAAGCAATAT+TGG - chr4.4:19535309-19535328 None:intergenic 30.0%
AAAGAACATGTTTGATGAGT+AGG + chr4.4:19535606-19535625 MS.gene35136:intron 30.0%
AACAATCAGATATGCACAAT+AGG - chr4.4:19535579-19535598 None:intergenic 30.0%
AACAGAGTTCAAGCAATATT+GGG - chr4.4:19535308-19535327 None:intergenic 30.0%
ACTAGAAAAATGATTGGAGA+TGG - chr4.4:19535282-19535301 None:intergenic 30.0%
ACTATTCAGTAACATCTTCA+AGG - chr4.4:19535470-19535489 None:intergenic 30.0%
AGACACAAAAGCCATTATAA+AGG - chr4.4:19536891-19536910 None:intergenic 30.0%
ATAAACATCAAAGCCTCTAA+GGG - chr4.4:19535436-19535455 None:intergenic 30.0%
ATAACAATCAAGTGAAGTCA+AGG - chr4.4:19536091-19536110 None:intergenic 30.0%
ATTCAACGATATTGACCATA+AGG - chr4.4:19536046-19536065 None:intergenic 30.0%
CCCAATTAGAAAAACATAGT+TGG - chr4.4:19536658-19536677 None:intergenic 30.0%
CGATTATGAAAGTAGGTAAT+TGG + chr4.4:19535826-19535845 MS.gene35136:intron 30.0%
GCTGTATAGGTTCAAAAATA+TGG + chr4.4:19535376-19535395 MS.gene35136:CDS 30.0%
GGGTTAACTAGAAAAATGAT+TGG - chr4.4:19535288-19535307 None:intergenic 30.0%
TAAGTCTAGGTTAAGAGTTT+AGG + chr4.4:19536605-19536624 MS.gene35136:intron 30.0%
TACCTACTTTCATAATCGTT+GGG - chr4.4:19535824-19535843 None:intergenic 30.0%
TCAGCTAGTTAAGACTTAAA+AGG + chr4.4:19536367-19536386 MS.gene35136:intron 30.0%
TTACCTACTTTCATAATCGT+TGG - chr4.4:19535825-19535844 None:intergenic 30.0%
TTGACCATAAGGTTATGATT+TGG - chr4.4:19536035-19536054 None:intergenic 30.0%
! ATTTTGTGGAGTTATCAACA+TGG - chr4.4:19536247-19536266 None:intergenic 30.0%
! CACTTGATTGTTATTAGCAA+GGG + chr4.4:19536097-19536116 MS.gene35136:intron 30.0%
! GATAAGTCATTCGAATTTTG+TGG - chr4.4:19536261-19536280 None:intergenic 30.0%
! TCACTTGATTGTTATTAGCA+AGG + chr4.4:19536096-19536115 MS.gene35136:intron 30.0%
! TTTACGTGTTTGTTACTCAA+GGG + chr4.4:19535648-19535667 MS.gene35136:intron 30.0%
! TTTTGTGGAGTTATCAACAT+GGG - chr4.4:19536246-19536265 None:intergenic 30.0%
!! GGTTTGAAATTCAAGTTGTT+TGG + chr4.4:19535896-19535915 MS.gene35136:intron 30.0%
AAACTCCTTTAGCATGCATA+GGG - chr4.4:19536469-19536488 None:intergenic 35.0%
AACATGTTTGATGAGTAGGA+AGG + chr4.4:19535610-19535629 MS.gene35136:intron 35.0%
AACTGAAAGTTACAGGATGT+TGG - chr4.4:19536328-19536347 None:intergenic 35.0%
AAGAGCTAACTGAAAGTTAC+AGG - chr4.4:19536335-19536354 None:intergenic 35.0%
ACCTACTTTCATAATCGTTG+GGG - chr4.4:19535823-19535842 None:intergenic 35.0%
ACGACCAAATCATAACCTTA+TGG + chr4.4:19536028-19536047 MS.gene35136:intron 35.0%
AGGCTTTCTCATTTGGTTAT+TGG - chr4.4:19536174-19536193 None:intergenic 35.0%
CAGAACATCAGTAAGTTCTT+TGG - chr4.4:19535525-19535544 None:intergenic 35.0%
CATAAACATCAAAGCCTCTA+AGG - chr4.4:19535437-19535456 None:intergenic 35.0%
GACTTATCCTCAATGCATAA+AGG + chr4.4:19536273-19536292 MS.gene35136:intron 35.0%
GGAACTCATAATACTCTACA+GGG - chr4.4:19536545-19536564 None:intergenic 35.0%
GGCTTTCTCATTTGGTTATT+GGG - chr4.4:19536173-19536192 None:intergenic 35.0%
GTAATTGGTCACTGGTAAAT+GGG + chr4.4:19535841-19535860 MS.gene35136:intron 35.0%
GTTGTTCATTCTCCTTGATT+TGG + chr4.4:19536926-19536945 MS.gene35136:intron 35.0%
TCAAGTGAAGTCAAGGATAA+TGG - chr4.4:19536084-19536103 None:intergenic 35.0%
TCTTGGAGCTATATAGCTAA+GGG + chr4.4:19536503-19536522 MS.gene35136:intron 35.0%
TCTTTCTCAGTGGATATTTC+TGG + chr4.4:19536834-19536853 MS.gene35136:intron 35.0%
TTCTTGGAGCTATATAGCTA+AGG + chr4.4:19536502-19536521 MS.gene35136:intron 35.0%
! AGACACGTGTTATTAAGTCT+AGG + chr4.4:19536592-19536611 MS.gene35136:intron 35.0%
! AGTTTTTCCTAGTGTCATAG+CGG + chr4.4:19537213-19537232 MS.gene35136:intron 35.0%
! CTTTACGTGTTTGTTACTCA+AGG + chr4.4:19535647-19535666 MS.gene35136:intron 35.0%
!! AGTACATGAAAGAGGCATTA+TGG - chr4.4:19536709-19536728 None:intergenic 35.0%
!!! ATGGGTGTTTTTGGATTTGT+TGG + chr4.4:19535859-19535878 MS.gene35136:intron 35.0%
!!! TTTTAGAGCATCTTTCTCAG+TGG + chr4.4:19536824-19536843 MS.gene35136:intron 35.0%
ACACACATGTACAGGATTAG+GGG - chr4.4:19536762-19536781 None:intergenic 40.0%
AGACCTGCATGTAGTTACTT+TGG - chr4.4:19536400-19536419 None:intergenic 40.0%
AGAGATTTGCGATGAATGAG+AGG - chr4.4:19536194-19536213 None:intergenic 40.0%
ATGTGTGTGCCAAGGAATAA+GGG + chr4.4:19536774-19536793 MS.gene35136:intron 40.0%
ATTTGGTCGTCCATGGTTAA+TGG - chr4.4:19536018-19536037 None:intergenic 40.0%
CACACACATGTACAGGATTA+GGG - chr4.4:19536763-19536782 None:intergenic 40.0%
CCTACTTTCATAATCGTTGG+GGG - chr4.4:19535822-19535841 None:intergenic 40.0%
CGGAACTCATAATACTCTAC+AGG - chr4.4:19536546-19536565 None:intergenic 40.0%
CTACGAACAACCATTAACCA+TGG + chr4.4:19536005-19536024 MS.gene35136:intron 40.0%
GAAACTCCTTTAGCATGCAT+AGG - chr4.4:19536470-19536489 None:intergenic 40.0%
GAAAGTAGGTAATTGGTCAC+TGG + chr4.4:19535833-19535852 MS.gene35136:intron 40.0%
GAATGAGAGGCTTTCTCATT+TGG - chr4.4:19536181-19536200 None:intergenic 40.0%
GAGTAACAAACACGTAAAGC+TGG - chr4.4:19535646-19535665 None:intergenic 40.0%
GATGGTGAAATCAAACCTGA+TGG - chr4.4:19535264-19535283 None:intergenic 40.0%
GGGTACTTTCATATTTGCAG+AGG - chr4.4:19536226-19536245 None:intergenic 40.0%
GGTAATTGGTCACTGGTAAA+TGG + chr4.4:19535840-19535859 MS.gene35136:intron 40.0%
! ACCTGCATGTAGTTACTTTG+GGG - chr4.4:19536398-19536417 None:intergenic 40.0%
! AGCAAGGGTTCATCTAGAAT+CGG + chr4.4:19536112-19536131 MS.gene35136:intron 40.0%
! CAACATTTTGCAAAGTTGCC+AGG - chr4.4:19537168-19537187 None:intergenic 40.0%
! CACTGGTAAATGGGTGTTTT+TGG + chr4.4:19535850-19535869 MS.gene35136:intron 40.0%
! GACCTGCATGTAGTTACTTT+GGG - chr4.4:19536399-19536418 None:intergenic 40.0%
! TACAGGGCATTACTTAGAGA+GGG - chr4.4:19536529-19536548 None:intergenic 40.0%
ACTTTCATAATCGTTGGGGG+AGG - chr4.4:19535819-19535838 None:intergenic 45.0%
CACACATGTACAGGATTAGG+GGG - chr4.4:19536761-19536780 None:intergenic 45.0%
CATGTGTGTGCCAAGGAATA+AGG + chr4.4:19536773-19536792 MS.gene35136:intron 45.0%
CCCCCAACGATTATGAAAGT+AGG + chr4.4:19535819-19535838 MS.gene35136:intron 45.0%
CTTTCATAATCGTTGGGGGA+GGG - chr4.4:19535818-19535837 None:intergenic 45.0%
GAACTCCCTATGCATGCTAA+AGG + chr4.4:19536461-19536480 MS.gene35136:intron 45.0%
GCACACACATGTACAGGATT+AGG - chr4.4:19536764-19536783 None:intergenic 45.0%
GCGCATGAACATCCAAATCA+AGG - chr4.4:19536941-19536960 None:intergenic 45.0%
TATGTGGTGTGCAATGCAGT+TGG + chr4.4:19537245-19537264 MS.gene35136:intron 45.0%
TGTGTGTGCCAAGGAATAAG+GGG + chr4.4:19536775-19536794 MS.gene35136:intron 45.0%
TTCATGCGCACACACACATA+TGG + chr4.4:19536952-19536971 MS.gene35136:intron 45.0%
TTCCTACAGACCTCACTCAA+GGG - chr4.4:19536444-19536463 None:intergenic 45.0%
TTTCATAATCGTTGGGGGAG+GGG - chr4.4:19535817-19535836 None:intergenic 45.0%
! CTACAGGGCATTACTTAGAG+AGG - chr4.4:19536530-19536549 None:intergenic 45.0%
! GGTGATAATGAAAGGCGACA+AGG + chr4.4:19535398-19535417 MS.gene35136:CDS 45.0%
! GTCTGAAACCCCTTATTCCT+TGG - chr4.4:19536786-19536805 None:intergenic 45.0%
! TCATCTAGAATCGGTGCAAG+AGG + chr4.4:19536121-19536140 MS.gene35136:intron 45.0%
!! ACAGGATGTTGGCTAGTCTT+CGG - chr4.4:19536317-19536336 None:intergenic 45.0%
!! ATAGCGGTACTGACAGTATG+TGG + chr4.4:19537229-19537248 MS.gene35136:intron 45.0%
!! GGTTATGATTTGGTCGTCCA+TGG - chr4.4:19536025-19536044 None:intergenic 45.0%
AAAGGCGACAAGGAGCTTGT+TGG + chr4.4:19535408-19535427 MS.gene35136:CDS 50.0%
AACAGCGAGATCACTGCTGA+GGG + chr4.4:19537020-19537039 MS.gene35136:intron 50.0%
ACCTCACTCAAGGGGTAGAT+GGG - chr4.4:19536435-19536454 None:intergenic 50.0%
CCATCACAGCTTCTTCCATC+AGG + chr4.4:19535246-19535265 MS.gene35136:CDS 50.0%
CCTGATGGAAGAAGCTGTGA+TGG - chr4.4:19535249-19535268 None:intergenic 50.0%
GAGCTGATTGACCGCTGTAT+AGG + chr4.4:19535363-19535382 MS.gene35136:intron 50.0%
GCCCCAAAGTAACTACATGC+AGG + chr4.4:19536394-19536413 MS.gene35136:intron 50.0%
GTCAGTACCGCTATGACACT+AGG - chr4.4:19537223-19537242 None:intergenic 50.0%
GTTCCTACAGACCTCACTCA+AGG - chr4.4:19536445-19536464 None:intergenic 50.0%
TCCTACAGACCTCACTCAAG+GGG - chr4.4:19536443-19536462 None:intergenic 50.0%
TCCTGTACATGTGTGTGCCA+AGG + chr4.4:19536766-19536785 MS.gene35136:intron 50.0%
TCCTTGGCACACACATGTAC+AGG - chr4.4:19536770-19536789 None:intergenic 50.0%
TTCATAATCGTTGGGGGAGG+GGG - chr4.4:19535816-19535835 None:intergenic 50.0%
!! CAGGATGTTGGCTAGTCTTC+GGG - chr4.4:19536316-19536335 None:intergenic 50.0%
!! GAGCTTGTTGGTACCCTTAG+AGG + chr4.4:19535420-19535439 MS.gene35136:CDS 50.0%
ACAGCGAGATCACTGCTGAG+GGG + chr4.4:19537021-19537040 MS.gene35136:intron 55.0%
ACCCATCTACCCCTTGAGTG+AGG + chr4.4:19536431-19536450 MS.gene35136:intron 55.0%
ACCCCTTGAGTGAGGTCTGT+AGG + chr4.4:19536439-19536458 MS.gene35136:intron 55.0%
AGATCACTGCTGAGGGGAGA+AGG + chr4.4:19537027-19537046 MS.gene35136:CDS 55.0%
CAACAGCGAGATCACTGCTG+AGG + chr4.4:19537019-19537038 MS.gene35136:intron 55.0%
CTGGATCAGGTGAACCACCA+GGG - chr4.4:19537272-19537291 None:intergenic 55.0%
GACCTCACTCAAGGGGTAGA+TGG - chr4.4:19536436-19536455 None:intergenic 55.0%
TCATAATCGTTGGGGGAGGG+GGG - chr4.4:19535815-19535834 None:intergenic 55.0%
TCTGGATCAGGTGAACCACC+AGG - chr4.4:19537273-19537292 None:intergenic 55.0%
TGTGCAATGCAGTTGGTCCC+TGG + chr4.4:19537252-19537271 MS.gene35136:intron 55.0%
!! GGCTAGTCTTCGGGCTATGA+AGG - chr4.4:19536307-19536326 None:intergenic 55.0%
GCAATGCAGTTGGTCCCTGG+TGG + chr4.4:19537255-19537274 MS.gene35136:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 19535234 19537296 19535234 ID=MS.gene35136
chr4.4 mRNA 19535234 19537296 19535234 ID=MS.gene35136.t1;Parent=MS.gene35136
chr4.4 exon 19535234 19535267 19535234 ID=MS.gene35136.t1.exon1;Parent=MS.gene35136.t1
chr4.4 CDS 19535234 19535267 19535234 ID=cds.MS.gene35136.t1;Parent=MS.gene35136.t1
chr4.4 exon 19535364 19535487 19535364 ID=MS.gene35136.t1.exon2;Parent=MS.gene35136.t1
chr4.4 CDS 19535364 19535487 19535364 ID=cds.MS.gene35136.t1;Parent=MS.gene35136.t1
chr4.4 exon 19537025 19537097 19537025 ID=MS.gene35136.t1.exon3;Parent=MS.gene35136.t1
chr4.4 CDS 19537025 19537097 19537025 ID=cds.MS.gene35136.t1;Parent=MS.gene35136.t1
chr4.4 exon 19537264 19537296 19537264 ID=MS.gene35136.t1.exon4;Parent=MS.gene35136.t1
chr4.4 CDS 19537264 19537296 19537264 ID=cds.MS.gene35136.t1;Parent=MS.gene35136.t1
Gene Sequence

>MS.gene35136

ATGGCTAATAATCCATCACAGCTTCTTCCATCAGGTTTGATTTCACCATCTCCAATCATTTTTCTAGTTAACCCAATATTGCTTGAACTCTGTTTAAGATGACATATCGTTTTGTGCTGAAAACATGCAGAGCTGATTGACCGCTGTATAGGTTCAAAAATATGGGTGATAATGAAAGGCGACAAGGAGCTTGTTGGTACCCTTAGAGGCTTTGATGTTTATGTTAACATGGTCCTTGAAGATGTTACTGAATAGTAAGACTTGCACTACCACATTTTATGCTTTTATCCAAAGAACTTACTGATGTTCTGTTTTTTTTTTTTTTTTTTTTTTTTTCACATTCCTATTGTGCATATCTGATTGTTTTTCTGTAAAGAACATGTTTGATGAGTAGGAAGGTAGAATAGAACCAGCTTTACGTGTTTGTTACTCAAGGGAGTCCCTCAAATTTTTAAAATGCATTTCTAACTGGAATTGTTATAATACATCAGTTTTAATAATTTACAGCTGTTTCCCCAACTTTTATGAATGTTTACATTTAATTTGAACTGGCATGATGTTCAAAAAGTTAATTATATCCCCCCTCCCCCAACGATTATGAAAGTAGGTAATTGGTCACTGGTAAATGGGTGTTTTTGGATTTGTTGGCACAAAAAAATATTGGTTTGAAATTCAAGTTGTTTGGGCAGATTTAGTTAAGATTTAAGAAAGTGAAGGGTTACTTGACTACTGATTTGTATGAACTCCTATCTTAATTTGAACTTTTGTGTGCTACGAACAACCATTAACCATGGACGACCAAATCATAACCTTATGGTCAATATCGTTGAATTGTTATGAAGAGCTACCATTATCCTTGACTTCACTTGATTGTTATTAGCAAGGGTTCATCTAGAATCGGTGCAAGAGGAGTCTTCCATATATTTACTTTCATTTCCCAATAACCAAATGAGAAAGCCTCTCATTCATCGCAAATCTCTTACTGCTATCCTCTGCAAATATGAAAGTACCCATGTTGATAACTCCACAAAATTCGAATGACTTATCCTCAATGCATAAAGGAAAAATTACCTTCATAGCCCGAAGACTAGCCAACATCCTGTAACTTTCAGTTAGCTCTTTAGTTACAAATGTCAGCTAGTTAAGACTTAAAAGGTCATGCCCCAAAGTAACTACATGCAGGTCTTAGTTAATGCAACCCATCTACCCCTTGAGTGAGGTCTGTAGGAACTCCCTATGCATGCTAAAGGAGTTTCAAATTTCTATGTTTCTTGGAGCTATATAGCTAAGGGCCCTCTCTAAGTAATGCCCTGTAGAGTATTATGAGTTCCGACATTGTCATCTTTTAAACTTTTTAGAGACACGTGTTATTAAGTCTAGGTTAAGAGTTTAGGTTTAGTTTATCATGGAAATACAGTACACCAACTATGTTTTTCTAATTGGGTGTTGTTTGATACATGTATAAAACAATGCCATAATGCCTCTTTCATGTACTAAGTTAATAGATAGACTATTTATATCTATCCCCCTAATCCTGTACATGTGTGTGCCAAGGAATAAGGGGTTTCAGACATCATTGCATATATGTTTTTTTAGAGCATCTTTCTCAGTGGATATTTCTGGTTATTTGATGTACTTACTGAAGACTTTCTTACCTTTATAATGGCTTTTGTGTCTTTATGTTTTATGTTAGTTGTTCATTCTCCTTGATTTGGATGTTCATGCGCACACACACATATGGTGATGCCTCACAAATTGATTTAGTTTATTGCTTTATTATTCATACAACAGCGAGATCACTGCTGAGGGGAGAAGGATAACTAAGCTTGATCAAATTTTACTCAATGGAAACAACATTGCCATTGTAAGTTTCAAATTCTCAATTGTTTTCTATTTACCTAAATTCTATGGTATATTAATAAATCATTTGACCTGGCAACTTTGCAAAATGTTGACAGTAATCTTCTCACATATAATTTAGTTTTTCCTAGTGTCATAGCGGTACTGACAGTATGTGGTGTGCAATGCAGTTGGTCCCTGGTGGTTCACCTGATCCAGAATGA
Protein sequence

>MS.gene35136.t1

MANNPSQLLPSELIDRCIGSKIWVIMKGDKELVGTLRGFDVYVNMVLEDVTEYEITAEGRRITKLDQILLNGNNIAILVPGGSPDPE