AlfalfaGEDB | MS.gene44813

M. sativa cultivar XinJiangDaYe / MS.gene44813

KEGG: K12622
GO: GO:0000375 GO:0000377 GO:0000398 GO:0000956 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0034641 GO:0034655 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0060255 GO:0065007 GO:0071704 GO:0090304 GO:1901360 GO:1901361 GO:1901575
PFAM: PF01423.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44813.t1	XP_003607812.1	100	98	0	0	1	98	1	98	1.90E-45	191.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44813.t1	Q9C6K5	91.5	94	8	0	5	98	4	97	2.9e-42	172.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44813.t1	G7JHJ2	100.0	98	0	0	1	98	1	98	1.4e-45	191.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene64000	MS.gene44813	PPI
MS.gene44813	MS.gene23567	PPI
MS.gene44813	MS.gene48145	PPI
MS.gene44813	MS.gene83541	PPI
MS.gene44813	MS.gene073734	PPI
MS.gene44813	MS.gene86409	PPI
MS.gene44813	MS.gene22977	PPI
MS.gene44813	MS.gene03346	PPI
MS.gene44813	MS.gene058567	PPI
MS.gene44813	MS.gene46845	PPI
MS.gene44813	MS.gene067412	PPI
MS.gene44813	MS.gene023131	PPI
MS.gene44813	MS.gene64202	PPI
MS.gene24174	MS.gene44813	PPI
MS.gene44813	MS.gene85722	PPI
MS.gene80364	MS.gene44813	PPI
MS.gene44813	MS.gene62660	PPI
MS.gene44813	MS.gene52741	PPI
MS.gene27050	MS.gene44813	PPI
MS.gene44813	MS.gene015624	PPI
MS.gene44813	MS.gene39725	PPI
MS.gene44813	MS.gene57277	PPI
MS.gene59503	MS.gene44813	PPI
MS.gene44813	MS.gene035486	PPI
MS.gene44813	MS.gene36469	PPI
MS.gene70342	MS.gene44813	PPI
MS.gene44813	MS.gene03315	PPI
MS.gene44813	MS.gene06981	PPI
MS.gene44813	MS.gene050311	PPI
MS.gene44813	MS.gene028322	PPI
MS.gene28122	MS.gene44813	PPI
MS.gene44813	MS.gene006962	PPI
MS.gene44813	MS.gene67483	PPI
MS.gene24734	MS.gene44813	PPI
MS.gene44813	MS.gene055162	PPI
MS.gene44813	MS.gene64804	PPI
MS.gene44813	MS.gene25097	PPI
MS.gene44813	MS.gene95848	PPI
MS.gene44813	MS.gene051172	PPI
MS.gene44813	MS.gene35136	PPI
MS.gene44813	MS.gene93333	PPI
MS.gene44813	MS.gene070731	PPI
MS.gene44813	MS.gene040757	PPI
MS.gene44813	MS.gene07102	PPI
MS.gene44813	MS.gene051245	PPI
MS.gene43700	MS.gene44813	PPI
MS.gene019784	MS.gene44813	PPI
MS.gene051241	MS.gene44813	PPI
MS.gene44813	MS.gene030751	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44813.t1	MTR_4g083170	100.000	98	0	0	1	98	1	98	2.95e-66	194

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44813.t1	AT1G76860	89.796	98	9	1	1	98	1	97	9.46e-59	175
MS.gene44813.t1	AT1G21190	86.458	96	13	0	3	98	2	97	7.72e-58	173

Find 18 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACATCTTAATATGATTCT+TGG	0.270259	5.4:+53645052	MS.gene44813:CDS
CGCCATCTCCCCTAACAAAA+AGG	0.295131	5.4:-53645623	None:intergenic
TAGGGGAGATGGCGTGATAT+TGG	0.403792	5.4:+53645632	MS.gene44813:CDS
AGACATATGAAGAAATTGTC+AGG	0.427463	5.4:+53645116	MS.gene44813:CDS
AAGCGCAGTGAAGGAGCCTT+TGG	0.436284	5.4:+53644865	MS.gene44813:CDS
AGAGGAAGAAAGCGCAGTGA+AGG	0.494057	5.4:+53644856	MS.gene44813:CDS
AGGAGCCTTTGGATCTCATA+AGG	0.521514	5.4:+53644876	MS.gene44813:CDS
AAGAAGAATGGCGGGGACAG+AGG	0.529954	5.4:+53644838	None:intergenic
AATTGAGAAAGAAGAATGGC+GGG	0.544172	5.4:+53644830	None:intergenic
CTCAGCCTTATGAGATCCAA+AGG	0.555367	5.4:-53644881	None:intergenic
CGTTCTGATAGAGAGCTTCG+CGG	0.598832	5.4:+53644932	MS.gene44813:CDS
CCATGTTATGCAGTCCTCAG+TGG	0.619717	5.4:-53645663	None:intergenic
GACATATGAAGAAATTGTCA+GGG	0.620564	5.4:+53645117	MS.gene44813:CDS
CGCGAAGCTCTCTATCAGAA+CGG	0.622836	5.4:-53644931	None:intergenic
TGTTATGCAGTCCTCAGTGG+TGG	0.628383	5.4:-53645660	None:intergenic
TCTGATTGCAGACTACAAAG+CGG	0.697746	5.4:+53645589	MS.gene44813:intron
ATTGAGAAAGAAGAATGGCG+GGG	0.698364	5.4:+53644831	None:intergenic
TATTGGTTTCTCCACCACTG+AGG	0.734600	5.4:+53645649	MS.gene44813:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAAGATTATTGTAGTTTA+AGG	+	chr5.4:53645251-53645270	MS.gene44813:intron	15.0%
!!!	TATTTTTTAGATTAGATTAC+TGG	+	chr5.4:53645373-53645392	MS.gene44813:intron	15.0%
!!!	ATCACAGTTTTGAAAAAATA+AGG	-	chr5.4:53645419-53645438	None:intergenic	20.0%
!!!	TAATTAATAAGCATTGTTGA+GGG	+	chr5.4:53645190-53645209	MS.gene44813:intron	20.0%
!!!	TTAATTAATAAGCATTGTTG+AGG	+	chr5.4:53645189-53645208	MS.gene44813:intron	20.0%
!	AGAAAAACAAAAATCATCCA+AGG	-	chr5.4:53645569-53645588	None:intergenic	25.0%
!	CAACATCTTAATATGATTCT+TGG	+	chr5.4:53645052-53645071	MS.gene44813:CDS	25.0%
!	CAATTTCAATTTCAATTTCC+AGG	+	chr5.4:53645021-53645040	MS.gene44813:intron	25.0%
!	TGAAAGAAAAAAGAGATGAA+AGG	-	chr5.4:53644984-53645003	None:intergenic	25.0%
!!	ATCTTTTATCAAACTGAAGA+AGG	-	chr5.4:53645239-53645258	None:intergenic	25.0%
!!	TACTTTCTACAATTTAGCTT+TGG	-	chr5.4:53645159-53645178	None:intergenic	25.0%
!!!	TTGTAGTTTAAGGTGTTTTA+AGG	+	chr5.4:53645261-53645280	MS.gene44813:intron	25.0%
	ACTCTGATTTAAGAACTATG+AGG	+	chr5.4:53645295-53645314	MS.gene44813:intron	30.0%
	AGACATATGAAGAAATTGTC+AGG	+	chr5.4:53645116-53645135	MS.gene44813:CDS	30.0%
	AGGTCAATAGTTGAAAGAAA+AGG	-	chr5.4:53645399-53645418	None:intergenic	30.0%
	GACATATGAAGAAATTGTCA+GGG	+	chr5.4:53645117-53645136	MS.gene44813:CDS	30.0%
!	AGTTCCATTCCTTTTTGTTA+GGG	+	chr5.4:53645614-53645633	MS.gene44813:CDS	30.0%
!	ATCATCCAAGGAAATTTTGA+TGG	-	chr5.4:53645557-53645576	None:intergenic	30.0%
	ACTTGGTCAAACAAATAACC+AGG	-	chr5.4:53645458-53645477	None:intergenic	35.0%
	CCAAGTTTCTATCGTTCTAT+TGG	+	chr5.4:53645472-53645491	MS.gene44813:intron	35.0%
	CCAATAGAACGATAGAAACT+TGG	-	chr5.4:53645475-53645494	None:intergenic	35.0%
	TAAGATGTTGATCATAAGCC+TGG	-	chr5.4:53645042-53645061	None:intergenic	35.0%
!	ATATCTACTAACTTGTGACC+TGG	+	chr5.4:53645437-53645456	MS.gene44813:intron	35.0%
!	CAGTTCCATTCCTTTTTGTT+AGG	+	chr5.4:53645613-53645632	MS.gene44813:CDS	35.0%
!	GTTCCATTCCTTTTTGTTAG+GGG	+	chr5.4:53645615-53645634	MS.gene44813:CDS	35.0%
!!	TCCAAGGAAATTTTGATGGT+TGG	-	chr5.4:53645553-53645572	None:intergenic	35.0%
!!!	AGGTGTTTTAAGGTTTTGAC+TGG	+	chr5.4:53645271-53645290	MS.gene44813:intron	35.0%
!!!	GGTGTTTTAAGGTTTTGACT+GGG	+	chr5.4:53645272-53645291	MS.gene44813:intron	35.0%
	GCCAACCATCAAAATTTCCT+TGG	+	chr5.4:53645549-53645568	MS.gene44813:intron	40.0%
	TCTCCCCTAACAAAAAGGAA+TGG	-	chr5.4:53645621-53645640	None:intergenic	40.0%
	TCTGATTGCAGACTACAAAG+CGG	+	chr5.4:53645589-53645608	MS.gene44813:intron	40.0%
!	TTCCTTTTTGTTAGGGGAGA+TGG	+	chr5.4:53645621-53645640	MS.gene44813:CDS	40.0%
	AGGAGCCTTTGGATCTCATA+AGG	+	chr5.4:53644876-53644895	MS.gene44813:CDS	45.0%
!	CTCAGCCTTATGAGATCCAA+AGG	-	chr5.4:53644884-53644903	None:intergenic	45.0%
!!	GAACTATGAGGCATTGACAC+AGG	+	chr5.4:53645307-53645326	MS.gene44813:intron	45.0%
!!	TATTGGTTTCTCCACCACTG+AGG	+	chr5.4:53645649-53645668	MS.gene44813:CDS	45.0%
!!	TTTTGATGGTTGGCGCAGAT+TGG	-	chr5.4:53645543-53645562	None:intergenic	45.0%
	CGCCATCTCCCCTAACAAAA+AGG	-	chr5.4:53645626-53645645	None:intergenic	50.0%
	CGCGAAGCTCTCTATCAGAA+CGG	-	chr5.4:53644934-53644953	None:intergenic	50.0%
	CGTTCTGATAGAGAGCTTCG+CGG	+	chr5.4:53644932-53644951	MS.gene44813:CDS	50.0%
	TAGGGGAGATGGCGTGATAT+TGG	+	chr5.4:53645632-53645651	MS.gene44813:CDS	50.0%
!	AGAGGAAGAAAGCGCAGTGA+AGG	+	chr5.4:53644856-53644875	MS.gene44813:CDS	50.0%
	AAGCGCAGTGAAGGAGCCTT+TGG	+	chr5.4:53644865-53644884	MS.gene44813:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	53644845	53645680	53644845	ID=MS.gene44813
chr5.4	mRNA	53644845	53645680	53644845	ID=MS.gene44813.t1;Parent=MS.gene44813
chr5.4	exon	53644845	53644964	53644845	ID=MS.gene44813.t1.exon1;Parent=MS.gene44813.t1
chr5.4	CDS	53644845	53644964	53644845	ID=cds.MS.gene44813.t1;Parent=MS.gene44813.t1
chr5.4	exon	53645043	53645138	53645043	ID=MS.gene44813.t1.exon2;Parent=MS.gene44813.t1
chr5.4	CDS	53645043	53645138	53645043	ID=cds.MS.gene44813.t1;Parent=MS.gene44813.t1
chr5.4	exon	53645600	53645680	53645600	ID=MS.gene44813.t1.exon3;Parent=MS.gene44813.t1
chr5.4	CDS	53645600	53645680	53645600	ID=cds.MS.gene44813.t1;Parent=MS.gene44813.t1

Gene Sequence

>MS.gene44813

ATGGCGGGGACAGAGGAAGAAAGCGCAGTGAAGGAGCCTTTGGATCTCATAAGGCTGAGTCTCGACGAACGTATCTACGTTAAACTCCGTTCTGATAGAGAGCTTCGCGGAAAACTTCATGTAATTTCTTCTACTTCCTTTCATCTCTTTTTTCTTTCACTGCTTGATTTCAATTTCAATTTCAATTTCAATTTCCAGGCTTATGATCAACATCTTAATATGATTCTTGGTGATGTTGAAGAAATTGTTACTACTGTTGAAATTGATGATGAGACATATGAAGAAATTGTCAGGGTAAGTCATAATCAAAACCAAAGCTAAATTGTAGAAAGTATATTTAAATGTTAATTAATAAGCATTGTTGAGGGTAGTATATGTCATTGATTGATTACCTTCTTCAGTTTGATAAAAGATTATTGTAGTTTAAGGTGTTTTAAGGTTTTGACTGGGACTCTGATTTAAGAACTATGAGGCATTGACACAGGCACATTGATGAGCATGATAGTAAAAGCTAGATACGTGTCTTGCTATTTTTTAGATTAGATTACTGGCCTTTTCTTTCAACTATTGACCTTATTTTTTCAAAACTGTGATATCTACTAACTTGTGACCTGGTTATTTGTTTGACCAAGTTTCTATCGTTCTATTGGAATTTTGTTCATATCTATAGTATACTATATGCATATTTACAATCTCCAATCTGCGCCAACCATCAAAATTTCCTTGGATGATTTTTGTTTTTCTTCTGATTGCAGACTACAAAGCGGACAGTTCCATTCCTTTTTGTTAGGGGAGATGGCGTGATATTGGTTTCTCCACCACTGAGGACTGCATAA

Protein sequence

>MS.gene44813.t1

MAGTEEESAVKEPLDLIRLSLDERIYVKLRSDRELRGKLHAYDQHLNMILGDVEEIVTTVEIDDETYEEIVRTTKRTVPFLFVRGDGVILVSPPLRTA