AlfalfaGEDB | MS.gene43700

M. sativa cultivar XinJiangDaYe / MS.gene43700

KEGG: K12622
GO: GO:0000375 GO:0000377 GO:0000398 GO:0000956 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0034641 GO:0034655 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0060255 GO:0065007 GO:0071704 GO:0090304 GO:1901360 GO:1901361 GO:1901575
PFAM: PF01423.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43700.t1	XP_003607812.1	100	98	0	0	1	98	1	98	1.90E-45	191.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43700.t1	Q9C6K5	91.5	94	8	0	5	98	4	97	2.9e-42	172.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43700.t1	G7JHJ2	100.0	98	0	0	1	98	1	98	1.4e-45	191.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene43700	MS.gene64804	PPI
MS.gene43700	MS.gene95848	PPI
MS.gene43700	MS.gene03346	PPI
MS.gene43700	MS.gene35136	PPI
MS.gene43700	MS.gene93333	PPI
MS.gene43700	MS.gene070731	PPI
MS.gene43700	MS.gene040757	PPI
MS.gene43700	MS.gene07102	PPI
MS.gene43700	MS.gene051245	PPI
MS.gene019784	MS.gene43700	PPI
MS.gene43700	MS.gene24734	PPI
MS.gene43700	MS.gene030751	PPI
MS.gene43700	MS.gene23567	PPI
MS.gene43700	MS.gene48145	PPI
MS.gene43700	MS.gene83541	PPI
MS.gene43700	MS.gene073734	PPI
MS.gene43700	MS.gene86409	PPI
MS.gene43700	MS.gene22977	PPI
MS.gene43700	MS.gene59503	PPI
MS.gene43700	MS.gene058567	PPI
MS.gene43700	MS.gene051241	PPI
MS.gene43700	MS.gene46845	PPI
MS.gene43700	MS.gene067412	PPI
MS.gene43700	MS.gene023131	PPI
MS.gene43700	MS.gene64202	PPI
MS.gene24174	MS.gene43700	PPI
MS.gene43700	MS.gene85722	PPI
MS.gene80364	MS.gene43700	PPI
MS.gene43700	MS.gene62660	PPI
MS.gene43700	MS.gene03315	PPI
MS.gene43700	MS.gene52741	PPI
MS.gene43700	MS.gene015624	PPI
MS.gene43700	MS.gene39725	PPI
MS.gene43700	MS.gene70342	PPI
MS.gene43700	MS.gene57277	PPI
MS.gene43700	MS.gene055162	PPI
MS.gene43700	MS.gene25097	PPI
MS.gene43700	MS.gene035486	PPI
MS.gene43700	MS.gene36469	PPI
MS.gene43700	MS.gene051172	PPI
MS.gene43700	MS.gene64000	PPI
MS.gene43700	MS.gene06981	PPI
MS.gene43700	MS.gene050311	PPI
MS.gene27050	MS.gene43700	PPI
MS.gene43700	MS.gene028322	PPI
MS.gene43700	MS.gene44813	PPI
MS.gene43700	MS.gene28122	PPI
MS.gene43700	MS.gene006962	PPI
MS.gene43700	MS.gene67483	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43700.t1	MTR_4g083170	100.000	98	0	0	1	98	1	98	2.95e-66	194

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43700.t1	AT1G76860	89.796	98	9	1	1	98	1	97	9.46e-59	175
MS.gene43700.t1	AT1G21190	86.458	96	13	0	3	98	2	97	7.72e-58	173

Find 18 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACATCTTAATATGATTCT+TGG	0.270259	5.1:+53311771	MS.gene43700:CDS
CGCCATCTCCCCTAACAAAA+AGG	0.290294	5.1:-53312434	None:intergenic
TAGGGGAGATGGCGTGATAT+TGG	0.403792	5.1:+53312443	MS.gene43700:CDS
AGACATATGAAGAAATTGTC+AGG	0.427463	5.1:+53311835	MS.gene43700:CDS
AAGCGCAGTGAAGGAGCCTT+TGG	0.436284	5.1:+53311583	MS.gene43700:CDS
AGAGGAAGAAAGCGCAGTGA+AGG	0.494057	5.1:+53311574	MS.gene43700:CDS
AGGAGCCTTTGGATCTCATA+AGG	0.521514	5.1:+53311594	MS.gene43700:CDS
AAGAAGAATGGCGGGGACAG+AGG	0.529954	5.1:+53311556	None:intergenic
AATTGAGAAAGAAGAATGGC+GGG	0.544172	5.1:+53311548	None:intergenic
CTCAGCCTTATGAGATCCAA+AGG	0.555367	5.1:-53311599	None:intergenic
CGTTCTGATAGAGAGCTTCG+CGG	0.598832	5.1:+53311650	MS.gene43700:CDS
CCATGTTATGCAGTCCTCAG+TGG	0.619717	5.1:-53312474	None:intergenic
GACATATGAAGAAATTGTCA+GGG	0.620564	5.1:+53311836	MS.gene43700:CDS
CGCGAAGCTCTCTATCAGAA+CGG	0.622836	5.1:-53311649	None:intergenic
TGTTATGCAGTCCTCAGTGG+TGG	0.628383	5.1:-53312471	None:intergenic
TCTGATTGCAGACTACAAAG+CGG	0.697746	5.1:+53312400	MS.gene43700:intron
ATTGAGAAAGAAGAATGGCG+GGG	0.698364	5.1:+53311549	None:intergenic
TATTGGTTTCTCCACCACTG+AGG	0.734600	5.1:+53312460	MS.gene43700:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAAGATTATTGTAGTTTA+AGG	+	chr5.1:53312011-53312030	MS.gene43700:intron	15.0%
!!!	TATTTTTTAGATTAGATTAC+TGG	+	chr5.1:53312133-53312152	MS.gene43700:intron	15.0%
!!!	TAATTAATAAGCATTGTTGA+GGG	+	chr5.1:53311950-53311969	MS.gene43700:intron	20.0%
!!!	TTAATTAATAAGCATTGTTG+AGG	+	chr5.1:53311949-53311968	MS.gene43700:intron	20.0%
!	AGAAAAACAAAAATCATCCA+AGG	-	chr5.1:53312380-53312399	None:intergenic	25.0%
!	CAACATCTTAATATGATTCT+TGG	+	chr5.1:53311771-53311790	MS.gene43700:CDS	25.0%
!	CAATTTCAATTTCAATTTCC+AGG	+	chr5.1:53311740-53311759	MS.gene43700:intron	25.0%
!!	ATCTTTTATCAAACTGAAGA+AGG	-	chr5.1:53311999-53312018	None:intergenic	25.0%
!!!	ATCACAGTTCTGAAAAAATA+AGG	-	chr5.1:53312179-53312198	None:intergenic	25.0%
!!!	TCTTTAAAACGATAGAAACT+TGG	-	chr5.1:53312235-53312254	None:intergenic	25.0%
!!!	TTGTAGTTTAAGGTGTTTTA+AGG	+	chr5.1:53312021-53312040	MS.gene43700:intron	25.0%
	AGACATATGAAGAAATTGTC+AGG	+	chr5.1:53311835-53311854	MS.gene43700:CDS	30.0%
	AGGTCAATAGTTGAAAGAAA+AGG	-	chr5.1:53312159-53312178	None:intergenic	30.0%
	AGTGTGAAAGAAAAAAGAGA+TGG	-	chr5.1:53311702-53311721	None:intergenic	30.0%
	GACATATGAAGAAATTGTCA+GGG	+	chr5.1:53311836-53311855	MS.gene43700:CDS	30.0%
	GATAAGAAAAGGATCATCTT+TGG	+	chr5.1:53312293-53312312	MS.gene43700:intron	30.0%
!	AAGATGATCCTTTTCTTATC+TGG	-	chr5.1:53312293-53312312	None:intergenic	30.0%
!	ACAGTTCTACAATTTAGCTT+TGG	-	chr5.1:53311878-53311897	None:intergenic	30.0%
!	AGATGATCCTTTTCTTATCT+GGG	-	chr5.1:53312292-53312311	None:intergenic	30.0%
!	ATCATCCAAGGAAATTTTGA+TGG	-	chr5.1:53312368-53312387	None:intergenic	30.0%
	ACTACATCCCAGATAAGAAA+AGG	+	chr5.1:53312282-53312301	MS.gene43700:intron	35.0%
	ACTCTGAGTTAAGAACTATG+AGG	+	chr5.1:53312055-53312074	MS.gene43700:intron	35.0%
	ACTTGGTCAAACAAATAACC+AGG	-	chr5.1:53312218-53312237	None:intergenic	35.0%
	TAAGATGTTGATCATAAGCC+TGG	-	chr5.1:53311761-53311780	None:intergenic	35.0%
!	AGTTCCGTTCCTTTTTGTTA+GGG	+	chr5.1:53312425-53312444	MS.gene43700:CDS	35.0%
!	ATATCTACTAACTTGTGACC+TGG	+	chr5.1:53312197-53312216	MS.gene43700:intron	35.0%
!!	TCCAAGGAAATTTTGATGGT+TGG	-	chr5.1:53312364-53312383	None:intergenic	35.0%
!!!	AGGTGTTTTAAGGTTTTGAC+TGG	+	chr5.1:53312031-53312050	MS.gene43700:intron	35.0%
!!!	GGTGTTTTAAGGTTTTGACT+GGG	+	chr5.1:53312032-53312051	MS.gene43700:intron	35.0%
	GCCAACCATCAAAATTTCCT+TGG	+	chr5.1:53312360-53312379	MS.gene43700:intron	40.0%
	TCTCCCCTAACAAAAAGGAA+CGG	-	chr5.1:53312432-53312451	None:intergenic	40.0%
	TCTGATTGCAGACTACAAAG+CGG	+	chr5.1:53312400-53312419	MS.gene43700:intron	40.0%
!	CAGTTCCGTTCCTTTTTGTT+AGG	+	chr5.1:53312424-53312443	MS.gene43700:CDS	40.0%
!	GTTCCGTTCCTTTTTGTTAG+GGG	+	chr5.1:53312426-53312445	MS.gene43700:CDS	40.0%
!	TTCCTTTTTGTTAGGGGAGA+TGG	+	chr5.1:53312432-53312451	MS.gene43700:CDS	40.0%
!!	AGAACTATGAGGCATTGACA+CGG	+	chr5.1:53312066-53312085	MS.gene43700:intron	40.0%
	AGGAGCCTTTGGATCTCATA+AGG	+	chr5.1:53311594-53311613	MS.gene43700:CDS	45.0%
!	CTCAGCCTTATGAGATCCAA+AGG	-	chr5.1:53311602-53311621	None:intergenic	45.0%
!!	GAACTATGAGGCATTGACAC+GGG	+	chr5.1:53312067-53312086	MS.gene43700:intron	45.0%
!!	TATTGGTTTCTCCACCACTG+AGG	+	chr5.1:53312460-53312479	MS.gene43700:CDS	45.0%
!!	TTTTGATGGTTGGCGCAGAT+TGG	-	chr5.1:53312354-53312373	None:intergenic	45.0%
	CGCCATCTCCCCTAACAAAA+AGG	-	chr5.1:53312437-53312456	None:intergenic	50.0%
	CGCGAAGCTCTCTATCAGAA+CGG	-	chr5.1:53311652-53311671	None:intergenic	50.0%
	CGTTCTGATAGAGAGCTTCG+CGG	+	chr5.1:53311650-53311669	MS.gene43700:CDS	50.0%
	TAGGGGAGATGGCGTGATAT+TGG	+	chr5.1:53312443-53312462	MS.gene43700:CDS	50.0%
!	AGAGGAAGAAAGCGCAGTGA+AGG	+	chr5.1:53311574-53311593	MS.gene43700:CDS	50.0%
	AAGCGCAGTGAAGGAGCCTT+TGG	+	chr5.1:53311583-53311602	MS.gene43700:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	53311563	53312491	53311563	ID=MS.gene43700
chr5.1	mRNA	53311563	53312491	53311563	ID=MS.gene43700.t1;Parent=MS.gene43700
chr5.1	exon	53311563	53311682	53311563	ID=MS.gene43700.t1.exon1;Parent=MS.gene43700.t1
chr5.1	CDS	53311563	53311682	53311563	ID=cds.MS.gene43700.t1;Parent=MS.gene43700.t1
chr5.1	exon	53311762	53311857	53311762	ID=MS.gene43700.t1.exon2;Parent=MS.gene43700.t1
chr5.1	CDS	53311762	53311857	53311762	ID=cds.MS.gene43700.t1;Parent=MS.gene43700.t1
chr5.1	exon	53312411	53312491	53312411	ID=MS.gene43700.t1.exon3;Parent=MS.gene43700.t1
chr5.1	CDS	53312411	53312491	53312411	ID=cds.MS.gene43700.t1;Parent=MS.gene43700.t1

Gene Sequence

>MS.gene43700

ATGGCGGGGACAGAGGAAGAAAGCGCAGTGAAGGAGCCTTTGGATCTCATAAGGCTGAGTCTCGACGAACGTATCTACGTTAAACTCCGTTCTGATAGAGAGCTTCGCGGAAAACTTCATGTAATTTCTTCTACTTCCATCTCTTTTTTCTTTCACACTTTTTTCACTGCTTGATTTCAATTTCAATTTCAATTTCCAGGCTTATGATCAACATCTTAATATGATTCTTGGTGATGTTGAAGAAATTGTTACTACTGTTGAAATTGATGATGAGACATATGAAGAAATTGTCAGGGTAAGTCATAATCAAAACCAAAGCTAAATTGTAGAACTGTGAACAGTTATTGATTTTTTTTTTTTTTAATTTTATATAGTATATTTAAATGTTAATTAATAAGCATTGTTGAGGGTAGTATATGTCATTGATTGATTACCTTCTTCAGTTTGATAAAAGATTATTGTAGTTTAAGGTGTTTTAAGGTTTTGACTGGGACTCTGAGTTAAGAACTATGAGGCATTGACACGGGCACATTGATGAGCATGATAGTAAAAGCTAGATACGTGTCTTGCTATTTTTTAGATTAGATTACTGGCCTTTTCTTTCAACTATTGACCTTATTTTTTCAGAACTGTGATATCTACTAACTTGTGACCTGGTTATTTGTTTGACCAAGTTTCTATCGTTTTAAAGAAAATTGTCTTTCTCTAAACAGTAGTACACTACATCCCAGATAAGAAAAGGATCATCTTTGGAATTTTGTTCATATCTATATGCATATTTACAATCTCCAATCTGCGCCAACCATCAAAATTTCCTTGGATGATTTTTGTTTTTCTTCTGATTGCAGACTACAAAGCGGACAGTTCCGTTCCTTTTTGTTAGGGGAGATGGCGTGATATTGGTTTCTCCACCACTGAGGACTGCATAA

Protein sequence

>MS.gene43700.t1

MAGTEEESAVKEPLDLIRLSLDERIYVKLRSDRELRGKLHAYDQHLNMILGDVEEIVTTVEIDDETYEEIVRTTKRTVPFLFVRGDGVILVSPPLRTA