AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032679

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032679.t1 MTR_6g004220 94.867 487 11 2 1 480 1 480 0.0 929
MS.gene032679.t1 MTR_6g004220 95.054 465 9 2 1 458 8 465 0.0 887
MS.gene032679.t1 MTR_6g004220 94.710 397 7 2 1 390 8 397 0.0 758
MS.gene032679.t1 MTR_7g082800 75.318 393 97 0 83 475 1 393 0.0 630
MS.gene032679.t1 MTR_6g004220 93.354 316 7 2 1 309 8 316 0.0 595
MS.gene032679.t1 MTR_7g082810 53.219 466 207 3 15 470 2 466 2.05e-176 504
MS.gene032679.t1 MTR_1g108840 48.247 485 242 3 1 478 13 495 2.11e-164 474
MS.gene032679.t1 MTR_1g108990 49.683 473 230 2 10 475 5 476 4.56e-163 470
MS.gene032679.t1 MTR_1g109060 50.000 466 224 3 10 468 5 468 2.41e-158 457
MS.gene032679.t1 MTR_1g109060 49.561 456 221 3 10 458 5 458 1.42e-152 443
MS.gene032679.t1 MTR_6g081410 47.939 461 230 3 15 466 10 469 4.83e-149 434
MS.gene032679.t1 MTR_6g081780 47.948 463 231 3 15 468 10 471 2.19e-148 432
MS.gene032679.t1 MTR_6g066380 47.788 452 225 4 33 475 30 479 2.72e-138 407
MS.gene032679.t1 MTR_6g081810 44.770 478 228 5 5 475 3 451 5.86e-133 392
MS.gene032679.t1 MTR_6g081300 42.641 462 253 3 20 474 4 460 2.82e-126 375
MS.gene032679.t1 MTR_1g108810 43.966 464 245 4 7 458 8 468 2.63e-125 374
MS.gene032679.t1 MTR_6g081400 43.919 444 242 1 30 466 15 458 5.62e-125 372
MS.gene032679.t1 MTR_8g106670 42.163 453 254 2 21 466 3 454 3.42e-122 365
MS.gene032679.t1 MTR_6g081240 39.683 441 258 2 25 458 5 444 3.43e-118 354
MS.gene032679.t1 MTR_6g081310 41.348 445 243 2 48 474 14 458 3.09e-116 350
MS.gene032679.t1 MTR_6g081390 41.667 444 252 1 30 466 12 455 8.03e-114 343
MS.gene032679.t1 MTR_2g088400 37.243 486 286 3 12 478 21 506 1.83e-108 331
MS.gene032679.t1 MTR_4g048000 35.812 511 294 4 1 477 7 517 1.79e-105 324
MS.gene032679.t1 MTR_2g022980 38.009 442 268 1 31 466 49 490 2.03e-98 305
MS.gene032679.t1 MTR_2g078930 36.667 480 287 5 7 471 12 489 3.43e-95 296
MS.gene032679.t1 MTR_2g088400 37.071 437 256 3 12 429 21 457 1.40e-94 294
MS.gene032679.t1 MTR_2g078960 36.638 464 284 3 1 457 6 466 7.26e-91 285
MS.gene032679.t1 MTR_2g079020 35.590 458 281 3 13 457 14 470 3.83e-88 278
MS.gene032679.t1 MTR_8g069470 34.783 483 301 5 6 476 25 505 1.12e-86 275
MS.gene032679.t1 MTR_2g078970 36.156 437 269 4 29 457 30 464 1.36e-83 266
MS.gene032679.t1 MTR_5g033100 35.650 446 278 3 28 466 54 497 1.39e-82 265
MS.gene032679.t1 MTR_5g032720 32.860 493 308 7 2 477 3 489 1.55e-80 258
MS.gene032679.t1 MTR_1g021740 34.565 460 290 5 15 466 20 476 3.96e-79 254
MS.gene032679.t1 MTR_7g087410 33.958 480 302 5 9 474 10 488 1.77e-77 251
MS.gene032679.t1 MTR_5g032750 34.947 475 283 10 4 463 12 475 7.18e-75 244
MS.gene032679.t1 MTR_1g101500 32.449 490 303 9 8 478 39 519 1.09e-74 244
MS.gene032679.t1 MTR_7g087430 36.719 384 242 1 83 466 1 383 1.21e-73 238
MS.gene032679.t1 MTR_7g087400 32.377 488 311 8 2 476 3 484 7.31e-73 238
MS.gene032679.t1 MTR_3g099700 33.811 488 309 6 1 479 20 502 8.21e-73 239
MS.gene032679.t1 MTR_0039s0080 31.489 470 308 4 5 464 13 478 2.25e-72 238
MS.gene032679.t1 MTR_8g063590 33.407 452 292 4 31 474 54 504 4.34e-72 237
MS.gene032679.t1 MTR_5g010830 31.590 478 304 8 1 464 37 505 9.82e-72 237
MS.gene032679.t1 MTR_7g087410 35.000 400 251 2 83 474 1 399 1.59e-71 232
MS.gene032679.t1 MTR_5g067460 30.022 463 310 5 13 464 36 495 2.34e-70 234
MS.gene032679.t1 MTR_5g090290 32.957 443 289 2 31 466 47 488 1.81e-69 232
MS.gene032679.t1 MTR_6g043810 30.754 491 309 7 12 475 2 488 2.02e-69 230
MS.gene032679.t1 MTR_6g043810 30.754 491 309 7 12 475 12 498 2.78e-69 230
MS.gene032679.t1 MTR_3g111020 29.025 472 317 6 5 464 26 491 1.07e-66 224
MS.gene032679.t1 MTR_7g087370 31.070 486 291 8 2 476 3 455 1.35e-66 221
MS.gene032679.t1 MTR_3g099720 31.453 461 303 4 25 474 32 490 5.06e-64 215
MS.gene032679.t1 MTR_1g100180 30.819 464 311 4 10 466 23 483 1.76e-61 209
MS.gene032679.t1 MTR_6g027200 31.780 472 300 8 9 466 14 477 1.01e-60 207
MS.gene032679.t1 MTR_4g027920 29.688 448 297 6 30 464 156 598 5.69e-59 205
MS.gene032679.t1 MTR_6g047640 30.867 473 310 8 7 466 3 471 8.12e-59 201
MS.gene032679.t1 MTR_8g102430 30.201 447 280 7 26 463 19 442 5.44e-58 198
MS.gene032679.t1 MTR_6g047610 28.151 476 318 7 7 466 3 470 5.83e-58 199
MS.gene032679.t1 MTR_6g047640 30.233 473 294 9 7 466 3 452 1.38e-57 197
MS.gene032679.t1 MTR_6g027190 30.042 476 310 5 1 466 3 465 1.31e-55 193
MS.gene032679.t1 MTR_2g078990 35.836 293 188 0 165 457 1 293 4.72e-55 187
MS.gene032679.t1 MTR_2g435930 28.846 468 309 4 29 473 18 484 8.84e-55 191
MS.gene032679.t1 MTR_5g087930 32.678 407 235 9 55 449 51 430 9.68e-55 190
MS.gene032679.t1 MTR_6g088805 27.557 479 307 9 3 463 7 463 1.39e-54 190
MS.gene032679.t1 MTR_5g011540 29.759 457 300 5 30 472 43 492 3.83e-51 181
MS.gene032679.t1 MTR_6g047590 29.747 474 313 7 7 466 3 470 1.90e-48 173
MS.gene032679.t1 MTR_5g090280 26.988 415 259 5 83 465 1 403 9.47e-40 148
MS.gene032679.t1 MTR_2g079030 38.462 195 120 0 263 457 319 513 2.04e-38 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032679.t1 AT1G66760 47.835 485 242 3 1 478 1 481 1.95e-170 489
MS.gene032679.t1 AT1G64820 49.688 481 232 3 4 475 2 481 2.78e-167 481
MS.gene032679.t1 AT1G66760 47.957 465 231 3 1 458 1 461 7.05e-163 469
MS.gene032679.t1 AT1G71140 49.786 468 223 3 9 469 8 470 5.07e-162 468
MS.gene032679.t1 AT1G66780 50.540 463 219 3 13 468 19 478 1.72e-158 458
MS.gene032679.t1 AT2G04090 49.007 453 223 2 27 472 26 477 1.01e-152 443
MS.gene032679.t1 AT2G04100 48.690 458 227 2 27 477 26 482 3.43e-152 442
MS.gene032679.t1 AT1G15170 46.895 467 235 4 8 466 14 475 7.49e-149 434
MS.gene032679.t1 AT1G15150 46.325 449 233 2 25 466 25 472 2.71e-148 432
MS.gene032679.t1 AT2G04080 44.561 478 253 3 8 478 4 476 4.03e-148 432
MS.gene032679.t1 AT2G04040 46.889 450 231 2 26 468 22 470 7.58e-148 431
MS.gene032679.t1 AT2G04070 47.085 446 228 2 31 469 27 471 2.06e-146 427
MS.gene032679.t1 AT2G04050 47.216 449 229 2 31 472 27 474 3.59e-146 427
MS.gene032679.t1 AT2G04100 48.975 439 216 2 27 458 26 463 6.59e-146 426
MS.gene032679.t1 AT5G52450 47.458 472 236 3 10 470 1 471 9.29e-146 426
MS.gene032679.t1 AT1G15160 45.880 449 235 2 25 466 25 472 1.60e-145 426
MS.gene032679.t1 AT2G04080 44.105 458 248 2 8 458 4 460 7.98e-142 415
MS.gene032679.t1 AT1G73700 47.510 482 238 4 1 475 1 474 4.17e-138 406
MS.gene032679.t1 AT2G34360 46.522 460 229 6 20 470 15 466 1.77e-137 405
MS.gene032679.t1 AT1G15180 44.968 467 244 4 8 466 15 476 2.28e-134 397
MS.gene032679.t1 AT1G66760 46.851 397 200 3 1 390 1 393 7.56e-132 387
MS.gene032679.t1 AT1G15150 47.875 353 184 0 114 466 4 356 2.44e-124 367
MS.gene032679.t1 AT1G15170 48.442 353 182 0 114 466 4 356 2.58e-123 364
MS.gene032679.t1 AT1G66760 49.850 333 167 0 126 458 1 333 2.59e-123 363
MS.gene032679.t1 AT2G34360 41.612 459 211 4 20 470 15 424 5.10e-116 348
MS.gene032679.t1 AT3G21690 36.383 481 289 2 3 477 35 504 1.34e-98 306
MS.gene032679.t1 AT1G61890 36.087 460 275 5 31 478 47 499 9.69e-95 296
MS.gene032679.t1 AT3G23550 36.797 462 272 5 12 457 6 463 1.15e-92 289
MS.gene032679.t1 AT1G61890 36.364 440 262 4 31 458 47 480 1.39e-91 287
MS.gene032679.t1 AT1G11670 33.598 503 306 6 1 477 1 501 3.20e-91 286
MS.gene032679.t1 AT3G23560 36.034 469 279 6 6 457 7 471 5.43e-89 280
MS.gene032679.t1 AT4G21903 38.313 415 243 4 61 466 83 493 5.54e-87 276
MS.gene032679.t1 AT3G26590 34.298 484 297 5 2 466 4 485 6.03e-87 275
MS.gene032679.t1 AT5G38030 35.590 458 286 3 28 478 42 497 7.38e-87 275
MS.gene032679.t1 AT4G21910 36.323 446 269 4 31 466 57 497 9.31e-87 275
MS.gene032679.t1 AT4G21910 36.323 446 269 4 31 466 57 497 9.31e-87 275
MS.gene032679.t1 AT4G21910 36.099 446 270 4 31 466 55 495 1.43e-86 275
MS.gene032679.t1 AT4G21910 36.099 446 270 4 31 466 55 495 1.43e-86 275
MS.gene032679.t1 AT4G21903 38.660 388 230 2 83 466 1 384 2.71e-86 270
MS.gene032679.t1 AT1G15180 46.429 280 150 0 111 390 1 280 7.29e-86 270
MS.gene032679.t1 AT1G12950 36.384 448 276 3 28 468 64 509 2.08e-85 272
MS.gene032679.t1 AT4G21903 37.209 430 253 5 61 477 83 508 1.37e-83 267
MS.gene032679.t1 AT3G59030 34.305 446 283 4 29 466 49 492 4.58e-80 258
MS.gene032679.t1 AT3G26590 37.500 384 239 1 83 466 1 383 3.92e-79 252
MS.gene032679.t1 AT1G61890 35.280 411 248 4 31 429 47 451 4.96e-79 254
MS.gene032679.t1 AT5G65380 34.947 475 292 8 3 466 10 478 3.25e-78 252
MS.gene032679.t1 AT1G23300 34.624 465 291 5 18 472 32 493 1.74e-77 251
MS.gene032679.t1 AT1G47530 34.382 477 300 6 1 466 1 475 4.75e-77 249
MS.gene032679.t1 AT1G33110 33.686 472 302 6 4 466 7 476 3.58e-76 247
MS.gene032679.t1 AT1G33100 34.115 469 301 5 4 466 7 473 1.68e-74 243
MS.gene032679.t1 AT5G44050 32.563 476 312 6 3 471 13 486 6.26e-74 241
MS.gene032679.t1 AT5G10420 33.755 474 298 6 3 466 12 479 1.17e-73 241
MS.gene032679.t1 AT1G33090 34.110 472 300 6 4 466 7 476 8.85e-73 238
MS.gene032679.t1 AT4G29140 31.950 482 312 5 2 474 31 505 9.71e-72 237
MS.gene032679.t1 AT1G33080 32.922 486 313 6 4 477 7 491 2.48e-70 232
MS.gene032679.t1 AT5G52050 32.158 482 299 9 1 463 7 479 3.95e-70 232
MS.gene032679.t1 AT1G47530 35.677 384 246 1 83 466 1 383 5.74e-70 228
MS.gene032679.t1 AT1G33080 33.333 468 303 5 4 463 7 473 1.06e-69 230
MS.gene032679.t1 AT3G23560 35.323 402 239 6 6 390 7 404 1.24e-69 228
MS.gene032679.t1 AT4G23030 31.884 483 311 5 9 476 8 487 5.31e-69 229
MS.gene032679.t1 AT4G00350 33.929 448 276 8 31 466 88 527 6.57e-66 221
MS.gene032679.t1 AT1G71870 31.172 478 298 8 12 462 6 479 2.07e-64 217
MS.gene032679.t1 AT2G38510 27.765 443 311 3 30 464 5 446 4.31e-64 215
MS.gene032679.t1 AT5G19700 30.670 463 299 7 30 476 35 491 6.22e-63 213
MS.gene032679.t1 AT1G58340 28.850 461 316 4 29 480 56 513 5.57e-61 208
MS.gene032679.t1 AT5G17700 30.064 469 321 3 4 466 7 474 1.39e-58 201
MS.gene032679.t1 AT5G17700 30.064 469 321 3 4 466 7 474 1.39e-58 201
MS.gene032679.t1 AT4G25640 31.712 473 308 4 6 466 4 473 3.79e-57 197
MS.gene032679.t1 AT3G03620 29.598 473 321 5 13 475 16 486 5.54e-57 197
MS.gene032679.t1 AT3G03620 29.598 473 321 5 13 475 16 486 5.54e-57 197
MS.gene032679.t1 AT4G25640 31.579 475 310 4 4 466 2 473 6.06e-57 197
MS.gene032679.t1 AT4G22790 31.180 449 295 5 26 463 25 470 2.57e-56 195
MS.gene032679.t1 AT5G49130 28.908 467 310 5 29 474 24 489 3.34e-56 195
MS.gene032679.t1 AT3G59030 32.703 370 239 4 29 390 49 416 1.59e-55 191
MS.gene032679.t1 AT1G33110 31.818 396 261 5 4 391 7 401 4.72e-52 181
MS.gene032679.t1 AT4G25640 31.469 429 279 4 6 422 4 429 2.07e-49 176
MS.gene032679.t1 AT2G04066 48.889 135 65 1 344 478 41 171 2.79e-37 135
MS.gene032679.t1 AT5G17700 27.226 393 279 3 4 390 7 398 1.53e-35 137

Find 102 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCTATTTGTTGGATTC+TGG 0.144927 6.3:+259858 MS.gene032679:CDS
ATTCCTATTGGTTTGTTATT+AGG 0.201827 6.3:+261250 MS.gene032679:CDS
TAGCTATTGGAATTTCTTAT+TGG 0.229128 6.3:+259917 MS.gene032679:CDS
TTCCTTCTGGACTCATGTTT+TGG 0.233890 6.3:+260055 MS.gene032679:CDS
TGCAAATATGCTATTCCTTC+TGG 0.235933 6.3:+260042 MS.gene032679:CDS
GTTAGAGTGGTTGTGATTAT+TGG 0.240480 6.3:+260557 MS.gene032679:CDS
ACTCGTGTTTCAAATGAATT+AGG 0.240783 6.3:+260503 MS.gene032679:CDS
TACCTCATTCCCGCTCTATT+TGG 0.243762 6.3:+259742 MS.gene032679:CDS
GTTAGTGCTGATAGTCTAAT+TGG 0.244051 6.3:+260707 MS.gene032679:CDS
TGTGAATTACATTGCGGATA+TGG 0.251319 6.3:+260664 MS.gene032679:CDS
AGTCTAATTGGAGCTCTTTC+AGG 0.262673 6.3:+260719 MS.gene032679:CDS
ACAGCTCCCTTAGCTGTCTT+TGG 0.262861 6.3:-260536 None:intergenic
AATATCCACACAAGAGATAT+TGG 0.269827 6.3:-259649 None:intergenic
GGTATACTTGTTTCATTCTC+TGG 0.275398 6.3:+258443 MS.gene032679:CDS
ATTGGAGCTGCTTTAGCTAT+TGG 0.308771 6.3:+259904 MS.gene032679:CDS
CTTATTGGTTGAATGTGATA+TGG 0.309579 6.3:+259932 MS.gene032679:CDS
GAGGTTTCAAGTTGAGGATT+AGG 0.328796 6.3:-260204 None:intergenic
GGTTGAATGTGATATGGCTT+TGG 0.339522 6.3:+259938 MS.gene032679:CDS
GTTGAAGAAAGTGAGTTTCA+TGG 0.346264 6.3:+258346 MS.gene032679:CDS
TATATTGTGGGAATTCCTAT+TGG 0.359116 6.3:+261238 MS.gene032679:CDS
AAAACCAGAATCCAACAAAT+AGG 0.359230 6.3:-259862 None:intergenic
TCAAGTGGTTTCTCTTATGA+TGG 0.367964 6.3:+258409 MS.gene032679:CDS
GTTGAATGTGATATGGCTTT+GGG 0.371323 6.3:+259939 MS.gene032679:CDS
ACCTTTGGTGCAGAGGAGTA+TGG 0.372023 6.3:+259571 MS.gene032679:CDS
TTGTGGATGGGAACTCTATC+AGG 0.380221 6.3:+261301 MS.gene032679:CDS
TTCTTCATTCCATATGCAAT+TGG 0.387110 6.3:+260360 MS.gene032679:CDS
AAACCTCGGTTCTTTCTGTC+TGG 0.401635 6.3:+260220 MS.gene032679:CDS
ATGAGTAGAAAACACAATCT+TGG 0.406242 6.3:-259995 None:intergenic
CTTATGATGGTGGGACATCT+TGG 0.426032 6.3:+258422 MS.gene032679:CDS
CGAGCTACATGAGACATTCC+AGG 0.426985 6.3:-259706 None:intergenic
AGGAGGTGGATTTCAAGAAA+TGG 0.431562 6.3:+261189 MS.gene032679:CDS
GAAAGATCATTCCCTGAGTC+TGG 0.432954 6.3:-259798 None:intergenic
AAACCTAATAACAAACCAAT+AGG 0.436422 6.3:-261253 None:intergenic
TTGTAACTGCATTAACTGAT+TGG 0.453816 6.3:+261353 MS.gene032679:CDS
GCATTGTAGTTGGGAATGGC+TGG 0.457500 6.3:+259523 MS.gene032679:intron
GGAGGTGGATTTCAAGAAAT+GGG 0.458931 6.3:+261190 MS.gene032679:CDS
ACACTATGTGGACAAACCTT+TGG 0.467875 6.3:+259556 MS.gene032679:CDS
CCTTGGAGCTTACTATATTG+TGG 0.467917 6.3:+261225 MS.gene032679:CDS
ATATCCACACAAGAGATATT+GGG 0.469336 6.3:-259648 None:intergenic
GCAGAAGCTCCAATTGCATA+TGG 0.475350 6.3:-260369 None:intergenic
CTTGGAGCTTACTATATTGT+GGG 0.480379 6.3:+261226 MS.gene032679:CDS
GTTTGCATTGTAGTTGGGAA+TGG 0.482865 6.3:+259519 MS.gene032679:intron
AGTGATGAGCATGGAAGTGA+TGG 0.493110 6.3:+258290 MS.gene032679:CDS
TCCATACTCCTCTGCACCAA+AGG 0.494105 6.3:-259572 None:intergenic
GAGTTTCATGGCAGCTCCAA+TGG 0.497826 6.3:+258358 MS.gene032679:CDS
GCAGGGGTTGCTAGAGGAGG+TGG 0.498862 6.3:+261175 MS.gene032679:intron
GATTCGCTATTTCCAGACTC+AGG 0.500456 6.3:+259786 MS.gene032679:CDS
AGTGGTTTCTCTTATGATGG+TGG 0.502914 6.3:+258412 MS.gene032679:CDS
GCAGAGGAGTATGGAAAACT+TGG 0.503242 6.3:+259580 MS.gene032679:CDS
CATCTAAAATTAAATGCGAA+GGG 0.503899 6.3:+261277 MS.gene032679:CDS
ATTAACTGATTGGCAGAAAG+AGG 0.504417 6.3:+261363 MS.gene032679:CDS
AATTAAATGCGAAGGGATTG+TGG 0.504525 6.3:+261284 MS.gene032679:CDS
GTTTCAAATGAATTAGGAGC+AGG 0.508966 6.3:+260509 MS.gene032679:CDS
AGAACCGAGGTTTCAAGTTG+AGG 0.511415 6.3:-260210 None:intergenic
TTCACAATATCTTCTTCAAG+TGG 0.512019 6.3:+258394 MS.gene032679:CDS
GTTGGGAATGGCTGGTGCAT+TGG 0.512611 6.3:+259531 MS.gene032679:intron
GTTTCCCAATATCTCTTGTG+TGG 0.512963 6.3:+259644 MS.gene032679:CDS
AAATGCGAAGGGATTGTGGA+TGG 0.515962 6.3:+261288 MS.gene032679:CDS
AAACTCTAGAGAGATGAGTA+AGG 0.519910 6.3:+258241 None:intergenic
CTAAAATATTTCTAAAGCAA+AGG 0.529386 6.3:-260613 None:intergenic
TCATCTAAAATTAAATGCGA+AGG 0.529668 6.3:+261276 MS.gene032679:CDS
TCATCACTCTTTCTTAGCAA+TGG 0.536715 6.3:-258275 None:intergenic
GAAACTGTTACAGCTACCAT+TGG 0.542723 6.3:-258374 None:intergenic
AACTAACAACACATTGGTGG+TGG 0.546469 6.3:+261547 MS.gene032679:CDS
GGAAATCCAAAGACAGCTAA+GGG 0.549690 6.3:+260530 MS.gene032679:CDS
AGGAAATCCAAAGACAGCTA+AGG 0.552623 6.3:+260529 MS.gene032679:CDS
GTGGTTTCTCTTATGATGGT+GGG 0.552777 6.3:+258413 MS.gene032679:CDS
CATTGCAGGCAACAAAAGCA+AGG 0.553104 6.3:+261498 MS.gene032679:intron
ATAGCATATTTGCAGAACTC+TGG 0.558784 6.3:-260033 None:intergenic
TCAGGGAATGATCTTTCCCA+TGG 0.560267 6.3:+259804 MS.gene032679:CDS
AGCCAAATAGAGCGGGAATG+AGG 0.570337 6.3:-259744 None:intergenic
TGGACAAACCTTTGGTGCAG+AGG 0.570653 6.3:+259564 MS.gene032679:CDS
TACCAAAACATGAGTCCAGA+AGG 0.573401 6.3:-260057 None:intergenic
ATTCGCTATTTCCAGACTCA+GGG 0.576880 6.3:+259787 MS.gene032679:CDS
CATATTTGCAGAACTCTGGT+AGG 0.578691 6.3:-260029 None:intergenic
AGCTAAGGGAGCTGTTAGAG+TGG 0.582031 6.3:+260544 MS.gene032679:CDS
TTGAGGATTAGGTAAAAGCC+CGG 0.583309 6.3:-260193 None:intergenic
TTCAGCAAAAGACATGGCAA+TGG 0.584361 6.3:-258471 None:intergenic
AGTGCATAGCCAAATAGAGC+GGG 0.584898 6.3:-259751 None:intergenic
CTAACAGAAACACAAAGAAG+AGG 0.588544 6.3:-260689 None:intergenic
AAGTTGGGATTAGGACACAT+TGG 0.599322 6.3:+259886 MS.gene032679:CDS
TAATCCTCAACTTGAAACCT+CGG 0.601964 6.3:+260206 MS.gene032679:CDS
ATGCAATTGGAGCTTCTGCA+AGG 0.601986 6.3:+260373 MS.gene032679:CDS
AGGCAACAAAAGCAAGGGAG+AGG 0.607625 6.3:+261504 MS.gene032679:intron
AATTAAAATAAGAATCTCAA+AGG 0.607660 6.3:-260169 None:intergenic
GAGCTACATGAGACATTCCA+GGG 0.620444 6.3:-259705 None:intergenic
AATGCGAAGGGATTGTGGAT+GGG 0.620482 6.3:+261289 MS.gene032679:CDS
CCACAATATAGTAAGCTCCA+AGG 0.626718 6.3:-261225 None:intergenic
TGAACTGCAGGGGTTGCTAG+AGG 0.635927 6.3:+261169 MS.gene032679:intron
CTAAGAAAGAGTGATGAGCA+TGG 0.642828 6.3:+258281 MS.gene032679:CDS
CTCTAGAGAGATGAGTAAGG+AGG 0.643848 6.3:+258244 None:intergenic
ACTGCAGGGGTTGCTAGAGG+AGG 0.647610 6.3:+261172 MS.gene032679:intron
GAGGTGGATTTCAAGAAATG+GGG 0.650274 6.3:+261191 MS.gene032679:CDS
AGTAACTTCAGCAAAAGACA+TGG 0.654565 6.3:-258477 None:intergenic
ATTGCAGGCAACAAAAGCAA+GGG 0.658902 6.3:+261499 MS.gene032679:intron
GGTGCATTGGAAACACTATG+TGG 0.664884 6.3:+259544 MS.gene032679:CDS
TGTGATTATTGGAATTGCTG+AGG 0.666119 6.3:+260568 MS.gene032679:CDS
AGGTGGATTTCAAGAAATGG+GGG 0.668457 6.3:+261192 MS.gene032679:CDS
ACAAGTTGTGAATTACATTG+CGG 0.676878 6.3:+260658 MS.gene032679:CDS
TGGAATGAAGAAGTGCAATG+AGG 0.693110 6.3:-260349 None:intergenic
AAGTGCATAGCCAAATAGAG+CGG 0.722956 6.3:-259752 None:intergenic
TCACCAGACAGAAAGAACCG+AGG 0.782249 6.3:-260223 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATGAATATACAAAA+GGG - chr6.3:259288-259307 None:intergenic 10.0%
!! TAGATTAAATTCATTAAATA+AGG - chr6.3:259378-259397 None:intergenic 10.0%
!! AATTAAAATAAGAATCTCAA+AGG - chr6.3:260172-260191 None:intergenic 15.0%
!! CAAAAAAATGAATATACAAA+AGG - chr6.3:259289-259308 None:intergenic 15.0%
!!! CTAGATTTTAATGTATATTT+TGG + chr6.3:261059-261078 MS.gene032679:intron 15.0%
!!! TTGTATATTCATTTTTTTGA+GGG + chr6.3:259290-259309 MS.gene032679:intron 15.0%
!!! TTTGTATATTCATTTTTTTG+AGG + chr6.3:259289-259308 MS.gene032679:intron 15.0%
!! AAAATCAGAAGAAGAAAAAT+AGG - chr6.3:258628-258647 None:intergenic 20.0%
!! AATAAAAACTAACAACACAT+TGG + chr6.3:261541-261560 MS.gene032679:CDS 20.0%
!! TATCACATGATATTATTGTT+AGG - chr6.3:260423-260442 None:intergenic 20.0%
!! TGTACAAATACAGAAAATTT+AGG - chr6.3:259199-259218 None:intergenic 20.0%
!!! AAAGTACTAATCAAACATTT+TGG - chr6.3:258672-258691 None:intergenic 20.0%
!!! AAATTTTCATTTACTTTCTG+AGG + chr6.3:260949-260968 MS.gene032679:intron 20.0%
!!! AATTTTCATTTACTTTCTGA+GGG + chr6.3:260950-260969 MS.gene032679:intron 20.0%
!!! CTAAAATATTTCTAAAGCAA+AGG - chr6.3:260616-260635 None:intergenic 20.0%
!!! CTTTGCTTTAGAAATATTTT+AGG + chr6.3:260614-260633 MS.gene032679:CDS 20.0%
!!! TTTTAGAATGAAGATATTTG+TGG - chr6.3:261108-261127 None:intergenic 20.0%
! AAACCTAATAACAAACCAAT+AGG - chr6.3:261256-261275 None:intergenic 25.0%
! AAGGTATAAATATTTAGACG+AGG - chr6.3:259359-259378 None:intergenic 25.0%
! AATATACAAAAGGGACATTA+AGG - chr6.3:259279-259298 None:intergenic 25.0%
! AATCCAAGAAAAACAAGAAA+AGG - chr6.3:258543-258562 None:intergenic 25.0%
! AATTCTCCTAGAAAATTATG+AGG + chr6.3:259017-259036 MS.gene032679:intron 25.0%
! ACTTTGTAACAGTAATAAAC+AGG + chr6.3:258687-258706 MS.gene032679:intron 25.0%
! AGTTGTGTGAATATAACAAT+AGG + chr6.3:260914-260933 MS.gene032679:intron 25.0%
! ATATACAAAAGGGACATTAA+GGG - chr6.3:259278-259297 None:intergenic 25.0%
! ATTCTCCTAGAAAATTATGA+GGG + chr6.3:259018-259037 MS.gene032679:intron 25.0%
! ATTTATAATGCATTGTCCTA+AGG + chr6.3:260261-260280 MS.gene032679:intron 25.0%
! CTTCACTCATTATATTCAAT+AGG - chr6.3:258807-258826 None:intergenic 25.0%
! GTTGTGTGAATATAACAATA+GGG + chr6.3:260915-260934 MS.gene032679:intron 25.0%
! TAGCTATTGGAATTTCTTAT+TGG + chr6.3:259917-259936 MS.gene032679:CDS 25.0%
! TATTGTTAGGATTATGTGTA+TGG - chr6.3:260410-260429 None:intergenic 25.0%
! TGAAAATATGAGGACTAATT+TGG + chr6.3:258969-258988 MS.gene032679:intron 25.0%
! TGCTCTAATATATAGTCATT+AGG + chr6.3:260860-260879 MS.gene032679:intron 25.0%
! TTATGAGGGTTGTATATAAA+TGG + chr6.3:259032-259051 MS.gene032679:intron 25.0%
! TTGTTGCAAAAAAACAAATG+TGG - chr6.3:261449-261468 None:intergenic 25.0%
! TTTATAATGCATTGTCCTAA+GGG + chr6.3:260262-260281 MS.gene032679:intron 25.0%
!! AAAGAAAAAAGTGCACAATT+TGG - chr6.3:261422-261441 None:intergenic 25.0%
!! ATCATTCTCAATTTTCTTGT+GGG + chr6.3:258837-258856 MS.gene032679:intron 25.0%
!! CATCTAAAATTAAATGCGAA+GGG + chr6.3:261277-261296 MS.gene032679:CDS 25.0%
!! TAGTCCTCATATTTTCATTT+TGG - chr6.3:258966-258985 None:intergenic 25.0%
!! TCATCTAAAATTAAATGCGA+AGG + chr6.3:261276-261295 MS.gene032679:CDS 25.0%
!! TTCTTCTTCTGATTTTTGTT+TGG + chr6.3:258632-258651 MS.gene032679:intron 25.0%
!!! AGATTCTTATTTTAATTGCC+GGG + chr6.3:260175-260194 MS.gene032679:CDS 25.0%
!!! ATTCCTATTGGTTTGTTATT+AGG + chr6.3:261250-261269 MS.gene032679:CDS 25.0%
!!! CAATATTGTGTAGTTTTGAA+TGG + chr6.3:260142-260161 MS.gene032679:intron 25.0%
!!! GAGATTCTTATTTTAATTGC+CGG + chr6.3:260174-260193 MS.gene032679:CDS 25.0%
!!! TGTTGTTAGTTTTTATTGAC+TGG - chr6.3:261538-261557 None:intergenic 25.0%
!!! TTCTGATTTTTGTTTGGATT+AGG + chr6.3:258638-258657 MS.gene032679:intron 25.0%
!!! TTGTTTTTTTGCAACAATTG+AGG + chr6.3:261452-261471 MS.gene032679:intron 25.0%
AAAAACTAACAACACATTGG+TGG + chr6.3:261544-261563 MS.gene032679:CDS 30.0%
AAAACCAGAATCCAACAAAT+AGG - chr6.3:259865-259884 None:intergenic 30.0%
AATATCCACACAAGAGATAT+TGG - chr6.3:259652-259671 None:intergenic 30.0%
ACAAGTTGTGAATTACATTG+CGG + chr6.3:260658-260677 MS.gene032679:CDS 30.0%
ACTCGTGTTTCAAATGAATT+AGG + chr6.3:260503-260522 MS.gene032679:CDS 30.0%
ATATAATGAATGTGAACTGC+AGG + chr6.3:261157-261176 MS.gene032679:intron 30.0%
ATATCCACACAAGAGATATT+GGG - chr6.3:259651-259670 None:intergenic 30.0%
ATGAGTAGAAAACACAATCT+TGG - chr6.3:259998-260017 None:intergenic 30.0%
CAAGAAAAACAAGAAAAGGA+AGG - chr6.3:258539-258558 None:intergenic 30.0%
CCATAAATCGTTGTATTAGA+GGG + chr6.3:258928-258947 MS.gene032679:intron 30.0%
GGATTTGTATACCATTCATT+TGG + chr6.3:259168-259187 MS.gene032679:intron 30.0%
GTCTCCAAAATGAAAATATG+AGG + chr6.3:258959-258978 MS.gene032679:intron 30.0%
TATAATGAATGTGAACTGCA+GGG + chr6.3:261158-261177 MS.gene032679:intron 30.0%
TATACAAAAGGGACATTAAG+GGG - chr6.3:259277-259296 None:intergenic 30.0%
TATATTGTGGGAATTCCTAT+TGG + chr6.3:261238-261257 MS.gene032679:CDS 30.0%
TCCATAAATCGTTGTATTAG+AGG + chr6.3:258927-258946 MS.gene032679:intron 30.0%
TTCACAATATCTTCTTCAAG+TGG + chr6.3:258394-258413 MS.gene032679:CDS 30.0%
TTCTTCATTCCATATGCAAT+TGG + chr6.3:260360-260379 MS.gene032679:CDS 30.0%
TTGTAACTGCATTAACTGAT+TGG + chr6.3:261353-261372 MS.gene032679:CDS 30.0%
! AGTACACATTCACGTTTTTA+GGG + chr6.3:261003-261022 MS.gene032679:intron 30.0%
! CTTATTGGTTGAATGTGATA+TGG + chr6.3:259932-259951 MS.gene032679:CDS 30.0%
! GATCATTCTCAATTTTCTTG+TGG + chr6.3:258836-258855 MS.gene032679:intron 30.0%
! TACAACCCTCATAATTTTCT+AGG - chr6.3:259026-259045 None:intergenic 30.0%
! TTCTTGTTTGCATTGTAGTT+GGG + chr6.3:259514-259533 MS.gene032679:intron 30.0%
! TTTAGGATTCTCCAAATGAA+TGG - chr6.3:259182-259201 None:intergenic 30.0%
! TTTCTTGTTTGCATTGTAGT+TGG + chr6.3:259513-259532 MS.gene032679:intron 30.0%
! TTTTGCACATTCCTATTTGT+TGG + chr6.3:259851-259870 MS.gene032679:CDS 30.0%
!!! ATCTTGGTTTTTTGACATGA+TGG - chr6.3:259982-260001 None:intergenic 30.0%
!!! CTTCCTTTTCTTGTTTTTCT+TGG + chr6.3:258537-258556 MS.gene032679:intron 30.0%
!!! TATTGTGTAGTTTTGAATGG+TGG + chr6.3:260145-260164 MS.gene032679:intron 30.0%
!!! TGGATTCTGGTTTTTAAGTT+GGG + chr6.3:259871-259890 MS.gene032679:CDS 30.0%
!!! TTGGATTCTGGTTTTTAAGT+TGG + chr6.3:259870-259889 MS.gene032679:CDS 30.0%
AACACAAACATCATGACATG+GGG - chr6.3:260472-260491 None:intergenic 35.0%
AATTAAATGCGAAGGGATTG+TGG + chr6.3:261284-261303 MS.gene032679:CDS 35.0%
ACAACACAAACATCATGACA+TGG - chr6.3:260474-260493 None:intergenic 35.0%
AGAGAGAAGAAGAACATACA+AGG - chr6.3:258514-258533 None:intergenic 35.0%
AGATACTGAGCTAAAAACCA+TGG - chr6.3:259824-259843 None:intergenic 35.0%
AGTAACTTCAGCAAAAGACA+TGG - chr6.3:258480-258499 None:intergenic 35.0%
ATAATGAATGTGAACTGCAG+GGG + chr6.3:261159-261178 MS.gene032679:intron 35.0%
ATAGCATATTTGCAGAACTC+TGG - chr6.3:260036-260055 None:intergenic 35.0%
ATTAACTGATTGGCAGAAAG+AGG + chr6.3:261363-261382 MS.gene032679:CDS 35.0%
CAACACAAACATCATGACAT+GGG - chr6.3:260473-260492 None:intergenic 35.0%
CCCTCTAATACAACGATTTA+TGG - chr6.3:258931-258950 None:intergenic 35.0%
CTAACAGAAACACAAAGAAG+AGG - chr6.3:260692-260711 None:intergenic 35.0%
CTTGGAGCTTACTATATTGT+GGG + chr6.3:261226-261245 MS.gene032679:CDS 35.0%
GACATTATGACTGTGAAATG+TGG - chr6.3:259317-259336 None:intergenic 35.0%
GATACTGAGCTAAAAACCAT+GGG - chr6.3:259823-259842 None:intergenic 35.0%
GGTATACTTGTTTCATTCTC+TGG + chr6.3:258443-258462 MS.gene032679:CDS 35.0%
GTGAAGACTATAGAGTAGTT+GGG + chr6.3:259054-259073 MS.gene032679:intron 35.0%
GTTAGAGTGGTTGTGATTAT+TGG + chr6.3:260557-260576 MS.gene032679:CDS 35.0%
GTTGAAGAAAGTGAGTTTCA+TGG + chr6.3:258346-258365 MS.gene032679:CDS 35.0%
GTTGAATGTGATATGGCTTT+GGG + chr6.3:259939-259958 MS.gene032679:CDS 35.0%
GTTTCAAATGAATTAGGAGC+AGG + chr6.3:260509-260528 MS.gene032679:CDS 35.0%
TAAACAGGATCTCATGTGAA+AGG + chr6.3:258702-258721 MS.gene032679:intron 35.0%
TAATCCTCAACTTGAAACCT+CGG + chr6.3:260206-260225 MS.gene032679:CDS 35.0%
TCAAGTGGTTTCTCTTATGA+TGG + chr6.3:258409-258428 MS.gene032679:CDS 35.0%
TCATCACTCTTTCTTAGCAA+TGG - chr6.3:258278-258297 None:intergenic 35.0%
TGCAAATATGCTATTCCTTC+TGG + chr6.3:260042-260061 MS.gene032679:CDS 35.0%
TGTACATCTCAACGATCTAA+CGG + chr6.3:259213-259232 MS.gene032679:intron 35.0%
TGTCACTCTTTGATCTTGTT+AGG - chr6.3:258741-258760 None:intergenic 35.0%
TGTGAATTACATTGCGGATA+TGG + chr6.3:260664-260683 MS.gene032679:CDS 35.0%
TGTGATTATTGGAATTGCTG+AGG + chr6.3:260568-260587 MS.gene032679:CDS 35.0%
TTTAGTATCAATGTCGTGTC+TGG + chr6.3:259435-259454 MS.gene032679:intron 35.0%
! ATCTAACGGCTCAAGTTTTA+GGG + chr6.3:259227-259246 MS.gene032679:intron 35.0%
! ATGTCTTTTGCTGAAGTTAC+TGG + chr6.3:258479-258498 MS.gene032679:intron 35.0%
! CAGTACACATTCACGTTTTT+AGG + chr6.3:261002-261021 MS.gene032679:intron 35.0%
! CATTCCTATTTGTTGGATTC+TGG + chr6.3:259858-259877 MS.gene032679:CDS 35.0%
!! GTTAGTGCTGATAGTCTAAT+TGG + chr6.3:260707-260726 MS.gene032679:CDS 35.0%
!!! CTGGTTTTTAAGTTGGGATT+AGG + chr6.3:259877-259896 MS.gene032679:CDS 35.0%
AACTAACAACACATTGGTGG+TGG + chr6.3:261547-261566 MS.gene032679:CDS 40.0%
AAGTGCATAGCCAAATAGAG+CGG - chr6.3:259755-259774 None:intergenic 40.0%
AAGTTGGGATTAGGACACAT+TGG + chr6.3:259886-259905 MS.gene032679:CDS 40.0%
ACACAAACATCATGACATGG+GGG - chr6.3:260471-260490 None:intergenic 40.0%
ACACTATGTGGACAAACCTT+TGG + chr6.3:259556-259575 MS.gene032679:CDS 40.0%
ACATTAAGGGGTTTGAATGG+TGG - chr6.3:259265-259284 None:intergenic 40.0%
ACATTGCATAGTTCACCCTT+AGG - chr6.3:260280-260299 None:intergenic 40.0%
AGGAAATCCAAAGACAGCTA+AGG + chr6.3:260529-260548 MS.gene032679:CDS 40.0%
AGTCTAATTGGAGCTCTTTC+AGG + chr6.3:260719-260738 MS.gene032679:CDS 40.0%
AGTGGTTTCTCTTATGATGG+TGG + chr6.3:258412-258431 MS.gene032679:CDS 40.0%
ATAGAGTAGTTGGGTATGGT+TGG + chr6.3:259063-259082 MS.gene032679:intron 40.0%
ATTGCAGGCAACAAAAGCAA+GGG + chr6.3:261499-261518 MS.gene032679:intron 40.0%
CATATTTGCAGAACTCTGGT+AGG - chr6.3:260032-260051 None:intergenic 40.0%
CATGTGATTGATTGCATTGC+AGG + chr6.3:261484-261503 MS.gene032679:intron 40.0%
CCACAATATAGTAAGCTCCA+AGG - chr6.3:261228-261247 None:intergenic 40.0%
CCTTGGAGCTTACTATATTG+TGG + chr6.3:261225-261244 MS.gene032679:CDS 40.0%
CTAAGAAAGAGTGATGAGCA+TGG + chr6.3:258281-258300 MS.gene032679:CDS 40.0%
CTACACGCAGAGTTGAAATA+AGG + chr6.3:259147-259166 MS.gene032679:intron 40.0%
CTTATTTCAACTCTGCGTGT+AGG - chr6.3:259149-259168 None:intergenic 40.0%
GAAACTGTTACAGCTACCAT+TGG - chr6.3:258377-258396 None:intergenic 40.0%
GACTATAGAGTAGTTGGGTA+TGG + chr6.3:259059-259078 MS.gene032679:intron 40.0%
GCTTAGAGATGCAAACTAGT+TGG - chr6.3:258577-258596 None:intergenic 40.0%
GGAAATCCAAAGACAGCTAA+GGG + chr6.3:260530-260549 MS.gene032679:CDS 40.0%
GGTGAAGACTATAGAGTAGT+TGG + chr6.3:259053-259072 MS.gene032679:intron 40.0%
GGTTGAATGTGATATGGCTT+TGG + chr6.3:259938-259957 MS.gene032679:CDS 40.0%
GTCGATGAATCGAGATTAGA+CGG - chr6.3:261086-261105 None:intergenic 40.0%
GTGGTTTCTCTTATGATGGT+GGG + chr6.3:258413-258432 MS.gene032679:CDS 40.0%
GTTTCCCAATATCTCTTGTG+TGG + chr6.3:259644-259663 MS.gene032679:CDS 40.0%
GTTTGCATTGTAGTTGGGAA+TGG + chr6.3:259519-259538 MS.gene032679:intron 40.0%
TACCAAAACATGAGTCCAGA+AGG - chr6.3:260060-260079 None:intergenic 40.0%
TTCAGCAAAAGACATGGCAA+TGG - chr6.3:258474-258493 None:intergenic 40.0%
TTGAGGATTAGGTAAAAGCC+CGG - chr6.3:260196-260215 None:intergenic 40.0%
! AGGAGGTGGATTTCAAGAAA+TGG + chr6.3:261189-261208 MS.gene032679:CDS 40.0%
! AGGTGGATTTCAAGAAATGG+GGG + chr6.3:261192-261211 MS.gene032679:CDS 40.0%
! ATTCGCTATTTCCAGACTCA+GGG + chr6.3:259787-259806 MS.gene032679:CDS 40.0%
! ATTGGAGCTGCTTTAGCTAT+TGG + chr6.3:259904-259923 MS.gene032679:CDS 40.0%
! GAGGTGGATTTCAAGAAATG+GGG + chr6.3:261191-261210 MS.gene032679:CDS 40.0%
! GATCTAACGGCTCAAGTTTT+AGG + chr6.3:259226-259245 MS.gene032679:intron 40.0%
! GGAGGTGGATTTCAAGAAAT+GGG + chr6.3:261190-261209 MS.gene032679:CDS 40.0%
! TGGAATGAAGAAGTGCAATG+AGG - chr6.3:260352-260371 None:intergenic 40.0%
! TTCCTTCTGGACTCATGTTT+TGG + chr6.3:260055-260074 MS.gene032679:CDS 40.0%
!! GAGGTTTCAAGTTGAGGATT+AGG - chr6.3:260207-260226 None:intergenic 40.0%
!!! CTGTTTTTTAGTCAAGACCC+TGG + chr6.3:259688-259707 MS.gene032679:CDS 40.0%
AAACCTCGGTTCTTTCTGTC+TGG + chr6.3:260220-260239 MS.gene032679:CDS 45.0%
AAATGCGAAGGGATTGTGGA+TGG + chr6.3:261288-261307 MS.gene032679:CDS 45.0%
AATGCGAAGGGATTGTGGAT+GGG + chr6.3:261289-261308 MS.gene032679:CDS 45.0%
AGTGATGAGCATGGAAGTGA+TGG + chr6.3:258290-258309 MS.gene032679:CDS 45.0%
AGTGCATAGCCAAATAGAGC+GGG - chr6.3:259754-259773 None:intergenic 45.0%
ATGCAATTGGAGCTTCTGCA+AGG + chr6.3:260373-260392 MS.gene032679:CDS 45.0%
ATTGGAGCTTCTGCAAGGTT+TGG + chr6.3:260378-260397 MS.gene032679:intron 45.0%
CATTGCAGGCAACAAAAGCA+AGG + chr6.3:261498-261517 MS.gene032679:intron 45.0%
GAAAGATCATTCCCTGAGTC+TGG - chr6.3:259801-259820 None:intergenic 45.0%
GAGCTACATGAGACATTCCA+GGG - chr6.3:259708-259727 None:intergenic 45.0%
GCAGAAGCTCCAATTGCATA+TGG - chr6.3:260372-260391 None:intergenic 45.0%
GGGACATTAAGGGGTTTGAA+TGG - chr6.3:259268-259287 None:intergenic 45.0%
GGTGCATTGGAAACACTATG+TGG + chr6.3:259544-259563 MS.gene032679:CDS 45.0%
GTAGGATCTTGTCACACAAC+AGG - chr6.3:259131-259150 None:intergenic 45.0%
TACCTCATTCCCGCTCTATT+TGG + chr6.3:259742-259761 MS.gene032679:CDS 45.0%
TCAGGGAATGATCTTTCCCA+TGG + chr6.3:259804-259823 MS.gene032679:CDS 45.0%
TTAGGGATGTCAGTGACTGT+TGG + chr6.3:261020-261039 MS.gene032679:intron 45.0%
TTGTGGATGGGAACTCTATC+AGG + chr6.3:261301-261320 MS.gene032679:CDS 45.0%
! AGCTTCTGCAAGGTTTGGTT+TGG + chr6.3:260383-260402 MS.gene032679:intron 45.0%
! GATTCGCTATTTCCAGACTC+AGG + chr6.3:259786-259805 MS.gene032679:CDS 45.0%
! GCAGAGGAGTATGGAAAACT+TGG + chr6.3:259580-259599 MS.gene032679:CDS 45.0%
!! AGAACCGAGGTTTCAAGTTG+AGG - chr6.3:260213-260232 None:intergenic 45.0%
!! CTTATGATGGTGGGACATCT+TGG + chr6.3:258422-258441 MS.gene032679:CDS 45.0%
ACAGCTCCCTTAGCTGTCTT+TGG - chr6.3:260539-260558 None:intergenic 50.0%
AGCCAAATAGAGCGGGAATG+AGG - chr6.3:259747-259766 None:intergenic 50.0%
AGCTAAGGGAGCTGTTAGAG+TGG + chr6.3:260544-260563 MS.gene032679:CDS 50.0%
AGGCAACAAAAGCAAGGGAG+AGG + chr6.3:261504-261523 MS.gene032679:intron 50.0%
ATGGGGGCTTATGTGAACCT+TGG + chr6.3:261208-261227 MS.gene032679:CDS 50.0%
CGAGCTACATGAGACATTCC+AGG - chr6.3:259709-259728 None:intergenic 50.0%
GAGTTTCATGGCAGCTCCAA+TGG + chr6.3:258358-258377 MS.gene032679:CDS 50.0%
GCATTGTAGTTGGGAATGGC+TGG + chr6.3:259523-259542 MS.gene032679:intron 50.0%
TCACCAGACAGAAAGAACCG+AGG - chr6.3:260226-260245 None:intergenic 50.0%
TCCATACTCCTCTGCACCAA+AGG - chr6.3:259575-259594 None:intergenic 50.0%
! ACCTTTGGTGCAGAGGAGTA+TGG + chr6.3:259571-259590 MS.gene032679:CDS 50.0%
! TGGACAAACCTTTGGTGCAG+AGG + chr6.3:259564-259583 MS.gene032679:CDS 50.0%
GTTGGGAATGGCTGGTGCAT+TGG + chr6.3:259531-259550 MS.gene032679:intron 55.0%
TGAACTGCAGGGGTTGCTAG+AGG + chr6.3:261169-261188 MS.gene032679:intron 55.0%
ACTGCAGGGGTTGCTAGAGG+AGG + chr6.3:261172-261191 MS.gene032679:intron 60.0%
GCAGGGGTTGCTAGAGGAGG+TGG + chr6.3:261175-261194 MS.gene032679:intron 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 258254 261574 258254 ID=MS.gene032679
chr6.3 mRNA 258254 261574 258254 ID=MS.gene032679.t1;Parent=MS.gene032679
chr6.3 exon 258254 258493 258254 ID=MS.gene032679.t1.exon1;Parent=MS.gene032679.t1
chr6.3 CDS 258254 258493 258254 ID=cds.MS.gene032679.t1;Parent=MS.gene032679.t1
chr6.3 exon 259532 260076 259532 ID=MS.gene032679.t1.exon2;Parent=MS.gene032679.t1
chr6.3 CDS 259532 260076 259532 ID=cds.MS.gene032679.t1;Parent=MS.gene032679.t1
chr6.3 exon 260155 260241 260155 ID=MS.gene032679.t1.exon3;Parent=MS.gene032679.t1
chr6.3 CDS 260155 260241 260155 ID=cds.MS.gene032679.t1;Parent=MS.gene032679.t1
chr6.3 exon 260338 260394 260338 ID=MS.gene032679.t1.exon4;Parent=MS.gene032679.t1
chr6.3 CDS 260338 260394 260338 ID=cds.MS.gene032679.t1;Parent=MS.gene032679.t1
chr6.3 exon 260502 260740 260502 ID=MS.gene032679.t1.exon5;Parent=MS.gene032679.t1
chr6.3 CDS 260502 260740 260502 ID=cds.MS.gene032679.t1;Parent=MS.gene032679.t1
chr6.3 exon 261179 261384 261179 ID=MS.gene032679.t1.exon6;Parent=MS.gene032679.t1
chr6.3 CDS 261179 261384 261179 ID=cds.MS.gene032679.t1;Parent=MS.gene032679.t1
chr6.3 exon 261506 261574 261506 ID=MS.gene032679.t1.exon7;Parent=MS.gene032679.t1
chr6.3 CDS 261506 261574 261506 ID=cds.MS.gene032679.t1;Parent=MS.gene032679.t1
Gene Sequence

>MS.gene032679

ATGAGTAAGGAGGTGACAACACCATTGCTAAGAAAGAGTGATGAGCATGGAAGTGATGGAAGAGTTGAGATTGAGAATACATTCATTCAAGAGTTGAAGAAAGTGAGTTTCATGGCAGCTCCAATGGTAGCTGTAACAGTTTCACAATATCTTCTTCAAGTGGTTTCTCTTATGATGGTGGGACATCTTGGTATACTTGTTTCATTCTCTGGTGTTTCCATTGCCATGTCTTTTGCTGAAGTTACTGGCTTTAGTGTCCTTGTATGTTCTTCTTCTCTCTCTCCTTCCTTTTCTTGTTTTTCTTGGATTCAACTATCAATCCAACTAGTTTGCATCTCTAAGCTTTGTATTTTGTTTTTATTTGTTTGAAGCCTATTTTTCTTCTTCTGATTTTTGTTTGGATTAGGAACATAATCCAAAATGTTTGATTAGTACTTTGTAACAGTAATAAACAGGATCTCATGTGAAAGGTATATAATGCAATCCTAACAAGATCAAAGAGTGACAACTATCTATTTGATTCTTGTACAGTAGAAAAATAGCATAGTATCCTATTGAATATAATGAGTGAAGATACAACTTGATCATTCTCAATTTTCTTGTGGGTGAATTCAATCTCTAACAATAAATAAACACAAATTAGTATGACATTTTCATATTCGAGACAAATTAGTCCATAAATCGTTGTATTAGAGGGACTAATTTGTCTCCAAAATGAAAATATGAGGACTAATTTGGATTTATTTTTTGTCAAGTAGTACTGAATTCTCCTAGAAAATTATGAGGGTTGTATATAAATGGTGAAGACTATAGAGTAGTTGGGTATGGTTGGTTGACAACACTTATTTTCTCATTGATGATGAATTGATGATGTCCTGTTGTGTGACAAGATCCTACACGCAGAGTTGAAATAAGGATTTGTATACCATTCATTTGGAGAATCCTAAATTTTCTGTATTTGTACATCTCAACGATCTAACGGCTCAAGTTTTAGGGAATGAATTATCTCCACCATTCAAACCCCTTAATGTCCCTTTTGTATATTCATTTTTTTGAGGGTCCACATTTCACAGTCATAATGTCACTTGACATAGTTATTTTTCCTCGTCTAAATATTTATACCTTATTTAATGAATTTAATCTAACTACTAATTTTTTTTTTTTCAAATTGTGACTGATGTTTTAGTATCAATGTCGTGTCTGGTGTGTGTGTCATCTGTGCTTCATAGTATAAATATAATGCTTATGCTTATTATGTGTTTCTTGTTTGCATTGTAGTTGGGAATGGCTGGTGCATTGGAAACACTATGTGGACAAACCTTTGGTGCAGAGGAGTATGGAAAACTTGGAAACTACACTTGCTGTGCAATATTAACTCTAACTGTAGTTTGTTTCCCAATATCTCTTGTGTGGATATTCACAGATAAAATACTACTGTTTTTTAGTCAAGACCCTGGAATGTCTCATGTAGCTCGCGAGTACTGCATATACCTCATTCCCGCTCTATTTGGCTATGCACTTCTTCAAGCGTTGATTCGCTATTTCCAGACTCAGGGAATGATCTTTCCCATGGTTTTTAGCTCAGTATCTGCTCTATTTTTGCACATTCCTATTTGTTGGATTCTGGTTTTTAAGTTGGGATTAGGACACATTGGAGCTGCTTTAGCTATTGGAATTTCTTATTGGTTGAATGTGATATGGCTTTGGGTTTATATTAAGTACTCTCCATCATGTCAAAAAACCAAGATTGTGTTTTCTACTCATGCTTTACATAACCTACCAGAGTTCTGCAAATATGCTATTCCTTCTGGACTCATGTTTTGGTAAATTATAAAACAATGCTGCTTCATTTCATTTTAATTTTCTTCTACTAATTTCAATTTTCATACAATATTGTGTAGTTTTGAATGGTGGTCCTTTGAGATTCTTATTTTAATTGCCGGGCTTTTACCTAATCCTCAACTTGAAACCTCGGTTCTTTCTGTCTGGTGAGTCTAATCAATTTCAATTTATAATGCATTGTCCTAAGGGTGAACTATGCAATGTGATCTTCTTTCTAATTTAATCTAAATTAACTCTTGCAGCTTGAACACTACCTCATTGCACTTCTTCATTCCATATGCAATTGGAGCTTCTGCAAGGTTTGGTTTGGTCCATACACATAATCCTAACAATAATATCATGTGATATTGCAAGATCTTCTGTTTTTGATATCCCCCATGTCATGATGTTTGTGTTGTTTTTGCAGTACTCGTGTTTCAAATGAATTAGGAGCAGGAAATCCAAAGACAGCTAAGGGAGCTGTTAGAGTGGTTGTGATTATTGGAATTGCTGAGGCAATTATTGTCAGCACTTTCTTCCTTTGCTTTAGAAATATTTTAGGATATGCTTATAGCAATGATGAACAAGTTGTGAATTACATTGCGGATATGGTTCCTCTTCTTTGTGTTTCTGTTAGTGCTGATAGTCTAATTGGAGCTCTTTCAGGTCAGTTTTATCTTTTATGTTATTAATAAGCTAGTCTTTTTGCTTAATTATTAGACTAATCTCTCAATTAGTGATCTCTCAAGTATTACATTTTCTTAGTTTCTAAACTATATGAAATCTGCTCTAATATATAGTCATTAGGATATATGAAAATCTTTCATGTTAGTCTCTAAAGTTGTGTGAATATAACAATAGGGATTAATGAGACAAATTTTCATTTACTTTCTGAGGGATTTAGATTTTGTTGCAGTTAGAAATCAACAGTACACATTCACGTTTTTAGGGATGTCAGTGACTGTTGGATCGAGATCAGACGATCTAGATTTTAATGTATATTTTGGACCGTCTAATCTCGATTCATCGACCACAAATATCTTCATTCTAAAAACATTATAGTTATATTTGTTACTTATATAATATAATGAATGTGAACTGCAGGGGTTGCTAGAGGAGGTGGATTTCAAGAAATGGGGGCTTATGTGAACCTTGGAGCTTACTATATTGTGGGAATTCCTATTGGTTTGTTATTAGGTTTTCATCTAAAATTAAATGCGAAGGGATTGTGGATGGGAACTCTATCAGGATCTGTTTTAAATGTCATTATACTATCTATTGTAACTGCATTAACTGATTGGCAGAAAGAGGTTTGTTTTCTTATACAAATACATAAAACTTTTTCCAAATTGTGCACTTTTTTCTTTTCAACCACATTTGTTTTTTTGCAACAATTGAGGCAACTTTTGCATGTGATTGATTGCATTGCAGGCAACAAAAGCAAGGGAGAGGATAATTGACCAGTCAATAAAAACTAACAACACATTGGTGGTGGCATGA
Protein sequence

>MS.gene032679.t1

MSKEVTTPLLRKSDEHGSDGRVEIENTFIQELKKVSFMAAPMVAVTVSQYLLQVVSLMMVGHLGILVSFSGVSIAMSFAELGMAGALETLCGQTFGAEEYGKLGNYTCCAILTLTVVCFPISLVWIFTDKILLFFSQDPGMSHVAREYCIYLIPALFGYALLQALIRYFQTQGMIFPMVFSSVSALFLHIPICWILVFKLGLGHIGAALAIGISYWLNVIWLWVYIKYSPSCQKTKIVFSTHALHNLPEFCKYAIPSGLMFCFEWWSFEILILIAGLLPNPQLETSVLSVCLNTTSLHFFIPYAIGASASTRVSNELGAGNPKTAKGAVRVVVIIGIAEAIIVSTFFLCFRNILGYAYSNDEQVVNYIADMVPLLCVSVSADSLIGALSGVARGGGFQEMGAYVNLGAYYIVGIPIGLLLGFHLKLNAKGLWMGTLSGSVLNVIILSIVTALTDWQKEATKARERIIDQSIKTNNTLVVA