AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049062

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049062.t1 MTR_8g102430 92.117 444 17 1 1 444 22 447 0.0 819
MS.gene049062.t1 MTR_6g088805 69.247 465 139 2 1 465 26 486 0.0 665
MS.gene049062.t1 MTR_5g067460 55.752 452 196 2 3 452 56 505 0.0 530
MS.gene049062.t1 MTR_3g111020 56.608 454 195 1 1 452 50 503 0.0 525
MS.gene049062.t1 MTR_1g101500 54.762 462 207 1 2 461 64 525 1.76e-178 511
MS.gene049062.t1 MTR_5g010830 55.405 444 196 1 1 442 64 507 3.07e-178 510
MS.gene049062.t1 MTR_0039s0080 55.000 460 203 2 2 459 38 495 9.92e-174 498
MS.gene049062.t1 MTR_6g043810 48.729 472 231 3 2 462 23 494 3.42e-158 458
MS.gene049062.t1 MTR_6g043810 48.729 472 231 3 2 462 33 504 4.09e-158 458
MS.gene049062.t1 MTR_4g027920 46.259 441 233 2 2 438 156 596 2.90e-140 416
MS.gene049062.t1 MTR_2g435930 43.284 469 242 3 1 451 18 480 1.80e-133 395
MS.gene049062.t1 MTR_1g108810 35.240 437 279 3 1 434 33 468 2.39e-83 265
MS.gene049062.t1 MTR_6g081400 35.068 442 282 3 2 439 15 455 1.67e-82 263
MS.gene049062.t1 MTR_6g081410 34.842 442 284 2 1 438 24 465 6.68e-82 261
MS.gene049062.t1 MTR_6g081780 34.163 442 287 2 1 438 24 465 2.03e-80 258
MS.gene049062.t1 MTR_1g108840 35.648 432 260 7 2 418 41 469 8.64e-80 256
MS.gene049062.t1 MTR_6g066380 34.169 439 283 4 5 438 30 467 8.45e-79 253
MS.gene049062.t1 MTR_5g011540 34.934 458 265 3 2 441 43 485 5.33e-78 252
MS.gene049062.t1 MTR_6g081810 32.648 438 271 3 1 438 26 439 4.58e-75 243
MS.gene049062.t1 MTR_6g081240 35.147 441 274 6 1 434 9 444 6.31e-74 239
MS.gene049062.t1 MTR_6g081300 34.014 441 287 2 1 438 9 448 7.89e-74 240
MS.gene049062.t1 MTR_6g081390 33.032 442 291 3 2 439 12 452 3.60e-72 236
MS.gene049062.t1 MTR_8g106670 31.828 443 292 3 3 439 13 451 2.07e-70 231
MS.gene049062.t1 MTR_7g082810 29.870 462 312 4 1 456 19 474 1.06e-69 229
MS.gene049062.t1 MTR_6g081310 35.198 429 262 4 25 438 19 446 5.59e-67 222
MS.gene049062.t1 MTR_2g078930 33.871 434 283 3 1 431 42 474 6.56e-67 223
MS.gene049062.t1 MTR_6g004220 30.856 444 300 4 1 439 22 463 1.34e-65 219
MS.gene049062.t1 MTR_2g079020 33.025 433 288 1 1 431 36 468 6.79e-64 215
MS.gene049062.t1 MTR_6g004220 30.752 439 297 4 1 434 29 465 9.79e-64 214
MS.gene049062.t1 MTR_2g078970 33.410 437 281 5 1 431 30 462 5.91e-63 212
MS.gene049062.t1 MTR_1g109060 29.638 442 305 4 2 439 24 463 2.09e-62 210
MS.gene049062.t1 MTR_2g078960 32.794 433 289 1 1 431 32 464 1.63e-61 208
MS.gene049062.t1 MTR_4g048000 30.524 439 303 2 3 439 65 503 1.45e-60 206
MS.gene049062.t1 MTR_2g088400 30.365 438 303 2 3 438 53 490 2.24e-60 206
MS.gene049062.t1 MTR_1g109060 29.519 437 302 4 2 434 24 458 3.63e-60 204
MS.gene049062.t1 MTR_1g108990 29.638 442 305 3 2 439 25 464 5.00e-60 204
MS.gene049062.t1 MTR_2g022980 28.246 439 313 2 3 439 49 487 2.51e-54 189
MS.gene049062.t1 MTR_2g088400 30.123 405 281 2 3 405 53 457 7.02e-53 184
MS.gene049062.t1 MTR_8g069470 29.888 445 301 5 1 439 49 488 2.09e-51 182
MS.gene049062.t1 MTR_7g082800 29.353 402 263 7 65 455 5 396 5.23e-49 172
MS.gene049062.t1 MTR_5g033100 27.563 439 315 2 3 439 57 494 1.55e-48 174
MS.gene049062.t1 MTR_5g032750 29.730 444 304 5 2 440 36 476 1.72e-47 171
MS.gene049062.t1 MTR_6g004220 29.661 354 246 2 1 351 29 382 5.87e-47 168
MS.gene049062.t1 MTR_5g032720 28.442 443 309 5 3 440 37 476 6.68e-47 169
MS.gene049062.t1 MTR_8g063590 28.537 417 295 2 25 438 76 492 4.08e-46 167
MS.gene049062.t1 MTR_1g021740 27.107 439 317 2 3 439 36 473 1.04e-45 166
MS.gene049062.t1 MTR_5g090290 28.281 442 307 4 3 438 47 484 7.07e-43 160
MS.gene049062.t1 MTR_3g099700 27.828 442 311 5 3 439 47 485 2.78e-39 149
MS.gene049062.t1 MTR_6g047610 27.765 443 305 9 2 437 31 465 1.89e-38 146
MS.gene049062.t1 MTR_1g100180 25.917 436 315 4 3 434 44 475 6.40e-38 145
MS.gene049062.t1 MTR_7g087400 25.591 465 320 6 2 451 30 483 9.27e-38 144
MS.gene049062.t1 MTR_3g099720 25.397 441 324 3 3 440 42 480 2.69e-36 140
MS.gene049062.t1 MTR_6g004220 30.034 293 200 3 1 290 29 319 3.01e-36 136
MS.gene049062.t1 MTR_6g047590 28.442 443 302 9 2 437 31 465 2.40e-34 134
MS.gene049062.t1 MTR_6g047640 26.862 443 291 9 2 437 31 447 2.03e-33 132
MS.gene049062.t1 MTR_6g047640 27.540 443 307 8 2 437 31 466 6.40e-33 130
MS.gene049062.t1 MTR_7g087410 24.336 452 315 7 2 439 31 469 7.44e-33 130
MS.gene049062.t1 MTR_5g087930 27.456 397 263 5 19 412 43 417 2.14e-32 129
MS.gene049062.t1 MTR_2g078990 31.164 292 191 5 146 431 4 291 2.88e-32 125
MS.gene049062.t1 MTR_7g087370 24.888 446 280 8 2 434 30 433 9.48e-31 124
MS.gene049062.t1 MTR_6g027190 25.110 454 309 7 2 439 24 462 6.41e-29 119
MS.gene049062.t1 MTR_7g087430 25.594 379 275 3 65 439 5 380 1.04e-28 117
MS.gene049062.t1 MTR_6g027200 24.841 471 321 8 2 453 36 492 2.50e-27 114
MS.gene049062.t1 MTR_7g087410 23.393 389 279 5 61 439 1 380 4.05e-27 112
MS.gene049062.t1 MTR_2g079030 32.642 193 130 0 239 431 319 511 3.73e-23 102
MS.gene049062.t1 MTR_5g090280 22.549 408 272 6 64 438 4 400 8.22e-15 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049062.t1 AT4G29140 65.116 473 154 3 1 462 54 526 0.0 634
MS.gene049062.t1 AT5G19700 65.022 446 154 1 2 445 35 480 0.0 612
MS.gene049062.t1 AT1G58340 57.461 449 189 1 1 447 56 504 1.34e-179 513
MS.gene049062.t1 AT5G52050 47.071 478 234 4 1 466 33 503 1.03e-160 464
MS.gene049062.t1 AT4G23030 53.947 456 207 2 1 453 30 485 1.10e-156 454
MS.gene049062.t1 AT1G71870 46.089 473 234 4 1 452 22 494 4.12e-153 445
MS.gene049062.t1 AT2G38510 47.939 461 236 3 2 458 5 465 1.87e-150 437
MS.gene049062.t1 AT5G49130 47.712 459 220 4 1 441 24 480 2.38e-140 412
MS.gene049062.t1 AT4G22790 36.461 469 274 5 1 452 28 489 2.42e-85 271
MS.gene049062.t1 AT5G52450 35.535 439 281 1 2 438 25 463 6.77e-79 254
MS.gene049062.t1 AT1G73700 35.307 456 281 4 2 452 23 469 9.19e-77 248
MS.gene049062.t1 AT1G15150 30.682 440 301 3 3 439 31 469 3.70e-69 228
MS.gene049062.t1 AT2G34360 31.591 440 291 3 2 438 26 458 9.56e-69 227
MS.gene049062.t1 AT1G71140 30.562 445 299 4 1 439 24 464 1.89e-68 226
MS.gene049062.t1 AT1G15170 31.818 440 296 3 3 439 34 472 7.29e-68 225
MS.gene049062.t1 AT1G66760 32.805 442 291 3 2 439 27 466 3.60e-67 223
MS.gene049062.t1 AT1G15160 30.000 440 304 3 3 439 31 469 3.69e-67 223
MS.gene049062.t1 AT1G66760 32.574 439 290 3 2 436 27 463 1.09e-65 219
MS.gene049062.t1 AT1G64820 29.932 441 307 1 1 439 27 467 2.89e-64 216
MS.gene049062.t1 AT1G66780 29.762 420 293 1 22 439 54 473 9.73e-63 211
MS.gene049062.t1 AT1G15180 31.973 441 294 5 3 439 35 473 5.52e-62 209
MS.gene049062.t1 AT2G04050 32.200 441 293 5 3 439 27 465 5.33e-60 204
MS.gene049062.t1 AT3G23550 30.184 434 299 3 1 431 29 461 1.47e-58 200
MS.gene049062.t1 AT1G61890 29.828 466 314 3 1 463 45 500 1.85e-57 198
MS.gene049062.t1 AT2G04040 29.613 439 307 1 3 439 27 465 2.29e-57 197
MS.gene049062.t1 AT3G23560 29.493 434 302 3 1 431 37 469 4.03e-57 196
MS.gene049062.t1 AT2G34360 28.929 439 262 2 2 438 26 416 4.07e-57 195
MS.gene049062.t1 AT2G04070 30.531 452 307 4 3 449 27 476 1.03e-56 195
MS.gene049062.t1 AT1G61890 30.435 437 300 2 1 434 45 480 1.61e-56 195
MS.gene049062.t1 AT1G11670 29.680 438 306 1 3 438 50 487 3.13e-56 194
MS.gene049062.t1 AT2G04080 30.820 451 305 6 3 448 27 475 4.24e-56 194
MS.gene049062.t1 AT4G21910 29.385 439 307 2 3 439 55 492 9.38e-56 193
MS.gene049062.t1 AT4G21910 29.385 439 307 2 3 439 55 492 9.38e-56 193
MS.gene049062.t1 AT4G21910 29.613 439 306 2 3 439 57 494 1.07e-55 193
MS.gene049062.t1 AT4G21910 29.613 439 306 2 3 439 57 494 1.07e-55 193
MS.gene049062.t1 AT2G04100 28.733 442 307 3 3 439 30 468 1.87e-55 192
MS.gene049062.t1 AT3G26590 28.702 439 310 2 3 439 45 482 7.49e-55 191
MS.gene049062.t1 AT2G04080 30.474 443 302 5 3 441 27 467 2.71e-54 189
MS.gene049062.t1 AT2G04100 28.604 437 304 3 3 434 30 463 6.30e-54 187
MS.gene049062.t1 AT2G04090 27.563 439 316 1 3 439 30 468 8.18e-54 187
MS.gene049062.t1 AT1G15170 30.484 351 240 3 92 439 4 353 3.77e-52 180
MS.gene049062.t1 AT3G21690 28.082 438 313 2 3 438 52 489 7.89e-52 183
MS.gene049062.t1 AT4G21903 29.340 409 286 2 33 439 83 490 9.93e-52 182
MS.gene049062.t1 AT5G38030 28.345 441 309 4 3 439 45 482 1.51e-51 182
MS.gene049062.t1 AT4G21903 29.557 406 283 2 33 436 83 487 2.30e-51 182
MS.gene049062.t1 AT1G61890 30.392 408 280 2 1 405 45 451 3.91e-50 177
MS.gene049062.t1 AT3G26590 29.365 378 264 2 64 439 4 380 8.64e-50 175
MS.gene049062.t1 AT1G15150 29.630 351 243 3 92 439 4 353 3.39e-49 172
MS.gene049062.t1 AT1G66760 32.791 369 242 3 2 366 27 393 4.47e-49 173
MS.gene049062.t1 AT1G23300 30.337 445 295 6 3 439 44 481 7.28e-49 175
MS.gene049062.t1 AT4G21903 29.443 377 264 1 65 439 5 381 1.15e-48 172
MS.gene049062.t1 AT4G00350 28.082 438 311 2 3 438 88 523 1.36e-48 175
MS.gene049062.t1 AT1G47530 28.217 443 309 6 2 439 34 472 7.10e-47 169
MS.gene049062.t1 AT3G23560 31.269 323 220 1 1 321 37 359 2.49e-46 166
MS.gene049062.t1 AT5G10420 28.118 441 313 3 3 440 38 477 5.81e-46 167
MS.gene049062.t1 AT3G59030 28.345 441 308 3 3 438 51 488 3.15e-45 165
MS.gene049062.t1 AT5G44050 28.281 442 311 4 3 440 40 479 1.04e-44 163
MS.gene049062.t1 AT1G12950 27.009 448 306 7 3 439 67 504 7.38e-44 162
MS.gene049062.t1 AT1G33090 28.111 434 305 3 3 432 36 466 1.48e-41 155
MS.gene049062.t1 AT1G66760 30.448 335 227 3 106 436 3 335 7.06e-41 149
MS.gene049062.t1 AT1G33100 29.545 440 291 8 3 432 33 463 7.15e-41 153
MS.gene049062.t1 AT4G25640 27.880 434 309 2 3 434 34 465 1.13e-40 152
MS.gene049062.t1 AT4G25640 27.880 434 309 2 3 434 34 465 1.97e-40 152
MS.gene049062.t1 AT5G65380 26.818 440 316 4 3 438 37 474 2.87e-40 151
MS.gene049062.t1 AT1G33110 27.273 440 301 8 3 432 36 466 1.82e-39 149
MS.gene049062.t1 AT1G47530 26.842 380 269 6 65 439 5 380 2.01e-37 141
MS.gene049062.t1 AT1G33080 27.074 458 310 8 2 446 35 481 2.06e-37 143
MS.gene049062.t1 AT4G25640 27.792 403 287 2 3 403 34 434 1.34e-36 140
MS.gene049062.t1 AT1G33080 27.191 445 301 7 2 434 35 468 2.19e-36 140
MS.gene049062.t1 AT3G03620 26.292 445 311 9 2 437 36 472 1.67e-33 132
MS.gene049062.t1 AT3G03620 26.292 445 311 9 2 437 36 472 1.67e-33 132
MS.gene049062.t1 AT3G59030 28.032 371 259 3 3 368 51 418 7.20e-33 129
MS.gene049062.t1 AT5G17700 26.398 447 312 10 2 439 33 471 1.38e-31 127
MS.gene049062.t1 AT5G17700 26.398 447 312 10 2 439 33 471 1.38e-31 127
MS.gene049062.t1 AT1G15180 30.827 266 178 5 92 353 4 267 1.61e-30 122
MS.gene049062.t1 AT1G33110 26.146 371 263 5 3 367 36 401 4.22e-27 113
MS.gene049062.t1 AT5G17700 25.776 322 226 8 2 316 33 348 3.87e-16 80.5

Find 163 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCGTAACCGTTGCTTCTA+TGG 0.153186 4.2:+2723187 MS.gene049062:CDS
GCAAGTGAGGCTAATGTAAT+TGG 0.227296 4.2:-2722879 None:intergenic
TGAGCCATGGTTGTGATCTT+TGG 0.229428 4.2:-2722765 None:intergenic
TCGGCCCAGGTTTGTTGTGC+TGG 0.242952 4.2:+2723653 MS.gene049062:CDS
ATCGCGTATGTTTGGTTAAC+TGG 0.250226 4.2:+2723011 MS.gene049062:CDS
CGGTGGGTTGCGGTGTTGTC+AGG 0.259072 4.2:+2723501 MS.gene049062:CDS
TATTCTTCGGCTGACTTCTT+TGG 0.260087 4.2:+2723433 MS.gene049062:CDS
TACCAGTCAACAACGGTTTC+TGG 0.265851 4.2:-2723787 None:intergenic
TGGTTGTGATCTTTGGGTTC+TGG 0.308597 4.2:-2722758 None:intergenic
TGGGTTTCGCGGGCTTTGGT+TGG 0.322674 4.2:+2723622 MS.gene049062:CDS
TTGGAAAAGCAAGCATCATT+AGG 0.333580 4.2:-2722434 None:intergenic
ATAAGAGCTGTTATGGCTAT+TGG 0.334848 4.2:-2722453 None:intergenic
GAGCCATGGTTGTGATCTTT+GGG 0.354470 4.2:-2722764 None:intergenic
AAGCTGCGTTTCCGTTTGTT+TGG 0.358580 4.2:+2723112 MS.gene049062:CDS
GAATCTTGGTGCTTTCTATA+TGG 0.360423 4.2:+2723556 MS.gene049062:CDS
AGGGTTAAGGATAGGAGTTT+AGG 0.362643 4.2:-2722648 None:intergenic
TGCAGAGGCAGCTGGGACTC+CGG 0.363910 4.2:-2722958 None:intergenic
TGGAAAAGCAAGCATCATTA+GGG 0.369962 4.2:-2722433 None:intergenic
ACATGTCACAAATCCTCATT+TGG 0.370537 4.2:+2722730 MS.gene049062:CDS
ATAGAAAATAAGAGCTGTTA+TGG 0.375099 4.2:-2722460 None:intergenic
TGGCGGTTGTGCTTGCGTTT+TGG 0.379139 4.2:+2723591 MS.gene049062:CDS
CACAGGGATGAGGTTTCGTT+GGG 0.383156 4.2:+2723385 MS.gene049062:CDS
CGGCCCAGGTTTGTTGTGCT+GGG 0.385823 4.2:+2723654 MS.gene049062:CDS
GTTGGGCTTGCTCTCGGCCC+AGG 0.386009 4.2:+2723640 MS.gene049062:CDS
TCTTTGGCACTTCCAATCCT+TGG 0.387985 4.2:+2723449 MS.gene049062:CDS
CCTCTCTGTTCACAAGCCTT+TGG 0.389078 4.2:+2722615 MS.gene049062:CDS
GTCTTTCACTTCGGTTTCAC+CGG 0.390820 4.2:+2722939 MS.gene049062:CDS
ACGTTGGGTTTCGCGGGCTT+TGG 0.391761 4.2:+2723618 MS.gene049062:CDS
ATTACATTAGCCTCACTTGC+TGG 0.391945 4.2:+2722882 MS.gene049062:CDS
GGGTTTCGCGGGCTTTGGTT+GGG 0.394583 4.2:+2723623 MS.gene049062:CDS
GTTAAGCCACATGAAGGAAA+TGG 0.397403 4.2:-2722709 None:intergenic
TTGGAGGCTGCAGAGGCAGC+TGG 0.401997 4.2:-2722966 None:intergenic
ATATTCGTCGCTGTAATGAT+AGG 0.402264 4.2:+2723341 MS.gene049062:CDS
ACCGTAACCGTTGCTTCTAT+GGG 0.420509 4.2:+2723188 MS.gene049062:CDS
CTGCTGATGAAAATATTCTT+CGG 0.422541 4.2:+2723420 MS.gene049062:CDS
TGGGAATGGAACATAGTAAC+AGG 0.424607 4.2:-2722689 None:intergenic
AAGACAAAGAAGTAATTTAG+TGG 0.425047 4.2:-2722921 None:intergenic
CACGAAGGTAAATACGTATA+GGG 0.425365 4.2:-2722839 None:intergenic
CCACAGGGATGAGGTTTCGT+TGG 0.426710 4.2:+2723384 MS.gene049062:CDS
AATGAATTTGTGATGAGATC+AGG 0.427364 4.2:-2722810 None:intergenic
CTTGGTGCTTTCTATATGGT+GGG 0.430875 4.2:+2723560 MS.gene049062:CDS
GTTCTCTCCGGTCTCTCTCT+CGG 0.430975 4.2:+2722585 MS.gene049062:CDS
TGTTCCTCATCGCGTATGTT+TGG 0.431746 4.2:+2723003 MS.gene049062:CDS
GCACGAAGGTAAATACGTAT+AGG 0.432233 4.2:-2722840 None:intergenic
AGAAGTAATTTAGTGGAAGA+TGG 0.436636 4.2:-2722914 None:intergenic
GGTGGGTTGCGGTGTTGTCA+GGG 0.445474 4.2:+2723502 MS.gene049062:CDS
GATGCAACGTTGAAGGGTTA+AGG 0.451908 4.2:-2722661 None:intergenic
TGAACCCAGCACAACAAACC+TGG 0.453660 4.2:-2723658 None:intergenic
GAAGGCGATGGCGAGTGAAC+CGG 0.458073 4.2:-2722541 None:intergenic
CCATTCCCATTTCCTTCATG+TGG 0.460327 4.2:+2722703 MS.gene049062:CDS
GCGTTTCCGTTTGTTTGGAA+TGG 0.460881 4.2:+2723117 MS.gene049062:CDS
TTGCTTACTTGTGTGGAGAA+TGG 0.463824 4.2:+2723734 MS.gene049062:CDS
TTCCATTCCGAGAGAGAGAC+CGG 0.465257 4.2:-2722592 None:intergenic
TTTAGGACGGTTTGCTCCAA+AGG 0.467132 4.2:-2722631 None:intergenic
TGGTTTGACGTTGGGTTTCG+CGG 0.472974 4.2:+2723611 MS.gene049062:CDS
GGTTCTGGTGCAACCAAATG+AGG 0.474606 4.2:-2722743 None:intergenic
ACCGGAGAGAACGGAGTAGC+CGG 0.474649 4.2:-2722574 None:intergenic
AATGGAATGGGTAGTGATGA+TGG 0.476279 4.2:+2723752 MS.gene049062:CDS
TTAAGCCACATGAAGGAAAT+GGG 0.477163 4.2:-2722708 None:intergenic
GCTTTGGTTGGGCTTGCTCT+CGG 0.477408 4.2:+2723634 MS.gene049062:CDS
TGAGGAACAACACAATGAAT+AGG 0.480105 4.2:-2722989 None:intergenic
TTCACATTCGCCGCCACACC+TGG 0.480133 4.2:-2723536 None:intergenic
ATGATGTTACTAGGCTATCT+AGG 0.481237 4.2:+2722501 MS.gene049062:CDS
AGTGATGATGGGATGATAAA+CGG 0.483280 4.2:+2723764 MS.gene049062:CDS
ATCGCCTTCGCCAACATCAC+CGG 0.484058 4.2:+2722555 MS.gene049062:CDS
CTCCGGTCTCTCTCTCGGAA+TGG 0.485898 4.2:+2722590 MS.gene049062:CDS
TGTCCTCAGACGGTGGGTTG+CGG 0.495604 4.2:+2723491 MS.gene049062:CDS
ACGCGTGTTGGAAACGAGCT+TGG 0.496589 4.2:+2723275 MS.gene049062:CDS
GTTTCCAACACGCGTGGAAA+CGG 0.498770 4.2:-2723267 None:intergenic
TATGTCTTTCCATCTTCACT+TGG 0.500525 4.2:+2723239 MS.gene049062:CDS
ATGTGATTGCGATCACTGAT+TGG 0.500968 4.2:+2723687 MS.gene049062:CDS
CCAAGCTAAGTTATCATCCC+TGG 0.503107 4.2:+2723313 MS.gene049062:CDS
CAAGTGAGGCTAATGTAATT+GGG 0.503400 4.2:-2722878 None:intergenic
GGAAGAGGATGCAACGTTGA+AGG 0.504510 4.2:-2722668 None:intergenic
CCCCATAGAAGCAACGGTTA+CGG 0.505664 4.2:-2723189 None:intergenic
CCAGGGATGATAACTTAGCT+TGG 0.506191 4.2:-2723313 None:intergenic
GTAGAGAAATTCTCTCCGGC+TGG 0.506195 4.2:+2723063 MS.gene049062:CDS
GGTTTGACGTTGGGTTTCGC+GGG 0.507344 4.2:+2723612 MS.gene049062:CDS
AACAGCAAGGCCAGGTGTGG+CGG 0.508991 4.2:+2723526 MS.gene049062:CDS
ACGGCTAATCCAAGTGAAGA+TGG 0.509085 4.2:-2723248 None:intergenic
TGTGATTGCGATCACTGATT+GGG 0.512724 4.2:+2723688 MS.gene049062:CDS
ATCCTTGGATTGTGTGAACT+TGG 0.512999 4.2:+2723464 MS.gene049062:CDS
ACCGGCTACTCCGTTCTCTC+CGG 0.514145 4.2:+2722573 MS.gene049062:CDS
CCACCACCATTCCAAACAAA+CGG 0.515374 4.2:-2723123 None:intergenic
TACTTCTTTGTCTTTCACTT+CGG 0.515725 4.2:+2722930 MS.gene049062:CDS
CGCAGCTTGGCGTGGCAAGT+CGG 0.517568 4.2:-2723097 None:intergenic
GTCAGGGGAACAGCAAGGCC+AGG 0.521102 4.2:+2723518 MS.gene049062:CDS
AACGCAAGCACAACCGCCAT+TGG 0.524005 4.2:-2723587 None:intergenic
TTTCCGTTTGTTTGGAATGG+TGG 0.524547 4.2:+2723120 MS.gene049062:CDS
CCGTTTGTTTGGAATGGTGG+TGG 0.525312 4.2:+2723123 MS.gene049062:CDS
GAGAGAGAGACCGGAGAGAA+CGG 0.527431 4.2:-2722583 None:intergenic
TCTTGGTGCTTTCTATATGG+TGG 0.529459 4.2:+2723559 MS.gene049062:CDS
TGGAGGCTGCAGAGGCAGCT+GGG 0.530384 4.2:-2722965 None:intergenic
GAGGAGAGTGCCAGCAAGTG+AGG 0.531668 4.2:-2722892 None:intergenic
AACGGAGTAGCCGGTGATGT+TGG 0.532013 4.2:-2722565 None:intergenic
TGGAACATAGTAACAGGAAG+AGG 0.532090 4.2:-2722683 None:intergenic
GGCCAGAAACCGTTGTTGAC+TGG 0.532184 4.2:+2723785 MS.gene049062:CDS
AGTTCACACAATCCAAGGAT+TGG 0.532587 4.2:-2723461 None:intergenic
TGACATGTTAAGCCACATGA+AGG 0.533931 4.2:-2722715 None:intergenic
CAGCGACGAATATTGCTACC+AGG 0.535583 4.2:-2723331 None:intergenic
GGCGTGGCAAGTCGGAGTAA+AGG 0.536544 4.2:-2723089 None:intergenic
CGTATTTACCTTCGTGCACA+AGG 0.537215 4.2:+2722846 MS.gene049062:CDS
GGTGATGTTGGCGAAGGCGA+TGG 0.540239 4.2:-2722553 None:intergenic
GAAGAGGATGCAACGTTGAA+GGG 0.547608 4.2:-2722667 None:intergenic
TAGATAGCCTAGTAACATCA+TGG 0.548657 4.2:-2722499 None:intergenic
CTATATGGTGGGGATGCCAA+TGG 0.550956 4.2:+2723571 MS.gene049062:CDS
TACCAAGTTCACACAATCCA+AGG 0.551400 4.2:-2723466 None:intergenic
GGGAACAGCAAGGCCAGGTG+TGG 0.554355 4.2:+2723523 MS.gene049062:CDS
GAACAACACAATGAATAGGT+TGG 0.554841 4.2:-2722985 None:intergenic
CCACATGAAGGAAATGGGAA+TGG 0.555412 4.2:-2722703 None:intergenic
GGAGAACTTGAACTTGCAGC+CGG 0.559934 4.2:+2722522 MS.gene049062:CDS
ATGAATTTGTGATGAGATCA+GGG 0.560522 4.2:-2722809 None:intergenic
TGCCCACTTGCTTACTTGTG+TGG 0.561216 4.2:+2723727 MS.gene049062:CDS
TGGTAACTGTCCTCAGACGG+TGG 0.568794 4.2:+2723484 MS.gene049062:CDS
ACACCGCAACCCACCGTCTG+AGG 0.569573 4.2:-2723494 None:intergenic
AGTAATTTAGTGGAAGATGG+AGG 0.572015 4.2:-2722911 None:intergenic
ATGGTGTCCATGATGTTACT+AGG 0.573020 4.2:+2722492 MS.gene049062:CDS
GAATAGGTTGGAGGCTGCAG+AGG 0.573910 4.2:-2722973 None:intergenic
CTGGCATACACTGCGCCACA+TGG 0.574182 4.2:+2723030 MS.gene049062:CDS
TTAGCCGTTTCCACGCGTGT+TGG 0.576266 4.2:+2723263 MS.gene049062:CDS
AACGTTGAAGGGTTAAGGAT+AGG 0.580029 4.2:-2722656 None:intergenic
GTGGCGGCGAATGTGAATCT+TGG 0.580181 4.2:+2723542 MS.gene049062:CDS
TAAAATCCCCATAGAAGCAA+CGG 0.581538 4.2:-2723195 None:intergenic
GTGGGTTGCGGTGTTGTCAG+GGG 0.581939 4.2:+2723503 MS.gene049062:CDS
ATAACTTAGCTTGGAAAGGA+CGG 0.583020 4.2:-2723304 None:intergenic
ATGGAATGGGTAGTGATGAT+GGG 0.586061 4.2:+2723753 MS.gene049062:CDS
AACGGAAACGCAGCTTGGCG+TGG 0.586430 4.2:-2723105 None:intergenic
GTTGACTGGTATTGTTGTTG+AGG 0.589869 4.2:+2723799 MS.gene049062:CDS
AAACAAACGGAAACGCAGCT+TGG 0.594222 4.2:-2723110 None:intergenic
GATGATAACTTAGCTTGGAA+AGG 0.597780 4.2:-2723308 None:intergenic
ATAGAAGCAACGGTTACGGT+GGG 0.602058 4.2:-2723185 None:intergenic
ACTTGTGTGGAGAATGGAAT+GGG 0.602809 4.2:+2723740 MS.gene049062:CDS
TTAAGGATAGGAGTTTAGGA+CGG 0.604091 4.2:-2722644 None:intergenic
TCGGAGTAAAGGCTTCCAGC+CGG 0.605342 4.2:-2723078 None:intergenic
CAACACAATGAATAGGTTGG+AGG 0.605721 4.2:-2722982 None:intergenic
CCGGAGAGAATTTCTCTACT+CGG 0.606415 4.2:-2723059 None:intergenic
CCGTAACCGTTGCTTCTATG+GGG 0.611433 4.2:+2723189 MS.gene049062:CDS
CATAGAAGCAACGGTTACGG+TGG 0.617561 4.2:-2723186 None:intergenic
TCTCCACACAAGTAAGCAAG+TGG 0.626487 4.2:-2723730 None:intergenic
GTAGCCGGTGATGTTGGCGA+AGG 0.633540 4.2:-2722559 None:intergenic
GAACCCAGCACAACAAACCT+GGG 0.635945 4.2:-2723657 None:intergenic
GGTAACTGTCCTCAGACGGT+GGG 0.641343 4.2:+2723485 MS.gene049062:CDS
CCGAGTAGAGAAATTCTCTC+CGG 0.642731 4.2:+2723059 MS.gene049062:CDS
TTGGTGCTTTCTATATGGTG+GGG 0.643884 4.2:+2723561 MS.gene049062:CDS
ACTTGGTAACTGTCCTCAGA+CGG 0.655240 4.2:+2723481 MS.gene049062:CDS
TCTACTCGGCGCTGTCCATG+TGG 0.656110 4.2:-2723045 None:intergenic
TAGTAACATCATGGACACCA+TGG 0.663160 4.2:-2722490 None:intergenic
AGCGACGAATATTGCTACCA+GGG 0.668909 4.2:-2723330 None:intergenic
TACTTGTGTGGAGAATGGAA+TGG 0.668929 4.2:+2723739 MS.gene049062:CDS
GAAGGTAAATACGTATAGGG+TGG 0.671979 4.2:-2722836 None:intergenic
TTAACCAAACATACGCGATG+AGG 0.672780 4.2:-2723007 None:intergenic
TATGGTGGGGATGCCAATGG+CGG 0.673877 4.2:+2723574 MS.gene049062:CDS
AAGCTCGTTTCCAACACGCG+TGG 0.684465 4.2:-2723273 None:intergenic
GTGTTGTCAGGGGAACAGCA+AGG 0.686076 4.2:+2723513 MS.gene049062:CDS
GAACCCAAAGATCACAACCA+TGG 0.692294 4.2:+2722761 MS.gene049062:CDS
CTCCACACAAGTAAGCAAGT+GGG 0.696610 4.2:-2723729 None:intergenic
GATTAGATATGTGTGAGCCA+TGG 0.701135 4.2:-2722778 None:intergenic
CCAAAGGCTTGTGAACAGAG+AGG 0.712249 4.2:-2722615 None:intergenic
ACAGGGATGAGGTTTCGTTG+GGG 0.713111 4.2:+2723386 MS.gene049062:CDS
TAGAAGCAACGGTTACGGTG+GGG 0.713451 4.2:-2723184 None:intergenic
GAGTGATTCCTTGTGCACGA+AGG 0.717544 4.2:-2722854 None:intergenic
ACAACAATACCAGTCAACAA+CGG 0.720742 4.2:-2723794 None:intergenic
CCAACGAAACCTCATCCCTG+TGG 0.753980 4.2:-2723384 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGACAAAGAAGTAATTTAG+TGG - chr4.2:2722924-2722943 None:intergenic 25.0%
! ATAGAAAATAAGAGCTGTTA+TGG - chr4.2:2722463-2722482 None:intergenic 25.0%
!! GAAGTAATGATCATTTTATG+TGG + chr4.2:2723149-2723168 MS.gene049062:CDS 25.0%
AATGAATTTGTGATGAGATC+AGG - chr4.2:2722813-2722832 None:intergenic 30.0%
AGAAGTAATTTAGTGGAAGA+TGG - chr4.2:2722917-2722936 None:intergenic 30.0%
ATGAATTTGTGATGAGATCA+GGG - chr4.2:2722812-2722831 None:intergenic 30.0%
CTGCTGATGAAAATATTCTT+CGG + chr4.2:2723420-2723439 MS.gene049062:CDS 30.0%
TACTTCTTTGTCTTTCACTT+CGG + chr4.2:2722930-2722949 MS.gene049062:CDS 30.0%
! CATTTTATGTGGTTTACTTG+TGG + chr4.2:2723160-2723179 MS.gene049062:CDS 30.0%
! TATTTTCTATTCTCGTTCCA+TGG + chr4.2:2722473-2722492 MS.gene049062:CDS 30.0%
ACAACAATACCAGTCAACAA+CGG - chr4.2:2723797-2723816 None:intergenic 35.0%
ACATGTCACAAATCCTCATT+TGG + chr4.2:2722730-2722749 MS.gene049062:CDS 35.0%
AGTAATTTAGTGGAAGATGG+AGG - chr4.2:2722914-2722933 None:intergenic 35.0%
ATAAGAGCTGTTATGGCTAT+TGG - chr4.2:2722456-2722475 None:intergenic 35.0%
ATATTCGTCGCTGTAATGAT+AGG + chr4.2:2723341-2723360 MS.gene049062:CDS 35.0%
ATGATGTTACTAGGCTATCT+AGG + chr4.2:2722501-2722520 MS.gene049062:CDS 35.0%
CAAGTGAGGCTAATGTAATT+GGG - chr4.2:2722881-2722900 None:intergenic 35.0%
CACGAAGGTAAATACGTATA+GGG - chr4.2:2722842-2722861 None:intergenic 35.0%
GAACAACACAATGAATAGGT+TGG - chr4.2:2722988-2723007 None:intergenic 35.0%
TAAAATCCCCATAGAAGCAA+CGG - chr4.2:2723198-2723217 None:intergenic 35.0%
TAGATAGCCTAGTAACATCA+TGG - chr4.2:2722502-2722521 None:intergenic 35.0%
TATGTCTTTCCATCTTCACT+TGG + chr4.2:2723239-2723258 MS.gene049062:CDS 35.0%
TGAGGAACAACACAATGAAT+AGG - chr4.2:2722992-2723011 None:intergenic 35.0%
TGGAAAAGCAAGCATCATTA+GGG - chr4.2:2722436-2722455 None:intergenic 35.0%
TTAAGCCACATGAAGGAAAT+GGG - chr4.2:2722711-2722730 None:intergenic 35.0%
TTAAGGATAGGAGTTTAGGA+CGG - chr4.2:2722647-2722666 None:intergenic 35.0%
TTGGAAAAGCAAGCATCATT+AGG - chr4.2:2722437-2722456 None:intergenic 35.0%
! ATAACTTAGCTTGGAAAGGA+CGG - chr4.2:2723307-2723326 None:intergenic 35.0%
! GATGATAACTTAGCTTGGAA+AGG - chr4.2:2723311-2723330 None:intergenic 35.0%
!! AATGATAGGTTTTTCAGCGA+CGG + chr4.2:2723355-2723374 MS.gene049062:CDS 35.0%
!! AGTGATGATGGGATGATAAA+CGG + chr4.2:2723764-2723783 MS.gene049062:CDS 35.0%
!! GAATCTTGGTGCTTTCTATA+TGG + chr4.2:2723556-2723575 MS.gene049062:CDS 35.0%
AACGTTGAAGGGTTAAGGAT+AGG - chr4.2:2722659-2722678 None:intergenic 40.0%
AATGGAATGGGTAGTGATGA+TGG + chr4.2:2723752-2723771 MS.gene049062:CDS 40.0%
ACTTGTGTGGAGAATGGAAT+GGG + chr4.2:2723740-2723759 MS.gene049062:CDS 40.0%
AGGGTTAAGGATAGGAGTTT+AGG - chr4.2:2722651-2722670 None:intergenic 40.0%
AGTTCACACAATCCAAGGAT+TGG - chr4.2:2723464-2723483 None:intergenic 40.0%
ATCCTTGGATTGTGTGAACT+TGG + chr4.2:2723464-2723483 MS.gene049062:CDS 40.0%
ATCGCGTATGTTTGGTTAAC+TGG + chr4.2:2723011-2723030 MS.gene049062:CDS 40.0%
ATGGAATGGGTAGTGATGAT+GGG + chr4.2:2723753-2723772 MS.gene049062:CDS 40.0%
ATGGTGTCCATGATGTTACT+AGG + chr4.2:2722492-2722511 MS.gene049062:CDS 40.0%
ATGTGATTGCGATCACTGAT+TGG + chr4.2:2723687-2723706 MS.gene049062:CDS 40.0%
GAAGGTAAATACGTATAGGG+TGG - chr4.2:2722839-2722858 None:intergenic 40.0%
GATTAGATATGTGTGAGCCA+TGG - chr4.2:2722781-2722800 None:intergenic 40.0%
GCAAGTGAGGCTAATGTAAT+TGG - chr4.2:2722882-2722901 None:intergenic 40.0%
GCACGAAGGTAAATACGTAT+AGG - chr4.2:2722843-2722862 None:intergenic 40.0%
GTTAAGCCACATGAAGGAAA+TGG - chr4.2:2722712-2722731 None:intergenic 40.0%
TACCAAGTTCACACAATCCA+AGG - chr4.2:2723469-2723488 None:intergenic 40.0%
TACTTGTGTGGAGAATGGAA+TGG + chr4.2:2723739-2723758 MS.gene049062:CDS 40.0%
TAGTAACATCATGGACACCA+TGG - chr4.2:2722493-2722512 None:intergenic 40.0%
TGACATGTTAAGCCACATGA+AGG - chr4.2:2722718-2722737 None:intergenic 40.0%
TGGAACATAGTAACAGGAAG+AGG - chr4.2:2722686-2722705 None:intergenic 40.0%
TGGGAATGGAACATAGTAAC+AGG - chr4.2:2722692-2722711 None:intergenic 40.0%
TTAACCAAACATACGCGATG+AGG - chr4.2:2723010-2723029 None:intergenic 40.0%
TTGCTTACTTGTGTGGAGAA+TGG + chr4.2:2723734-2723753 MS.gene049062:CDS 40.0%
! ATTACATTAGCCTCACTTGC+TGG + chr4.2:2722882-2722901 MS.gene049062:CDS 40.0%
! CAACACAATGAATAGGTTGG+AGG - chr4.2:2722985-2723004 None:intergenic 40.0%
! GTTGACTGGTATTGTTGTTG+AGG + chr4.2:2723799-2723818 MS.gene049062:CDS 40.0%
! TATTCTTCGGCTGACTTCTT+TGG + chr4.2:2723433-2723452 MS.gene049062:CDS 40.0%
! TGTGATTGCGATCACTGATT+GGG + chr4.2:2723688-2723707 MS.gene049062:CDS 40.0%
! TTGGTGCTTTCTATATGGTG+GGG + chr4.2:2723561-2723580 MS.gene049062:CDS 40.0%
! TTTCCGTTTGTTTGGAATGG+TGG + chr4.2:2723120-2723139 MS.gene049062:CDS 40.0%
!! CTTGGTGCTTTCTATATGGT+GGG + chr4.2:2723560-2723579 MS.gene049062:CDS 40.0%
!! TCTTGGTGCTTTCTATATGG+TGG + chr4.2:2723559-2723578 MS.gene049062:CDS 40.0%
!!! TTGCGTTTTGGTTTGACGTT+GGG + chr4.2:2723603-2723622 MS.gene049062:CDS 40.0%
AAACAAACGGAAACGCAGCT+TGG - chr4.2:2723113-2723132 None:intergenic 45.0%
AAGCTGCGTTTCCGTTTGTT+TGG + chr4.2:2723112-2723131 MS.gene049062:CDS 45.0%
ACCGTAACCGTTGCTTCTAT+GGG + chr4.2:2723188-2723207 MS.gene049062:CDS 45.0%
ACGGCTAATCCAAGTGAAGA+TGG - chr4.2:2723251-2723270 None:intergenic 45.0%
AGCGACGAATATTGCTACCA+GGG - chr4.2:2723333-2723352 None:intergenic 45.0%
ATAGAAGCAACGGTTACGGT+GGG - chr4.2:2723188-2723207 None:intergenic 45.0%
CCAAGCTAAGTTATCATCCC+TGG + chr4.2:2723313-2723332 MS.gene049062:CDS 45.0%
CCACATGAAGGAAATGGGAA+TGG - chr4.2:2722706-2722725 None:intergenic 45.0%
CCACCACCATTCCAAACAAA+CGG - chr4.2:2723126-2723145 None:intergenic 45.0%
CCAGGGATGATAACTTAGCT+TGG - chr4.2:2723316-2723335 None:intergenic 45.0%
CCATTCCCATTTCCTTCATG+TGG + chr4.2:2722703-2722722 MS.gene049062:CDS 45.0%
CCGAGTAGAGAAATTCTCTC+CGG + chr4.2:2723059-2723078 MS.gene049062:CDS 45.0%
CCGGAGAGAATTTCTCTACT+CGG - chr4.2:2723062-2723081 None:intergenic 45.0%
CGTATTTACCTTCGTGCACA+AGG + chr4.2:2722846-2722865 MS.gene049062:CDS 45.0%
CTCCACACAAGTAAGCAAGT+GGG - chr4.2:2723732-2723751 None:intergenic 45.0%
GAACCCAAAGATCACAACCA+TGG + chr4.2:2722761-2722780 MS.gene049062:CDS 45.0%
GAAGAGGATGCAACGTTGAA+GGG - chr4.2:2722670-2722689 None:intergenic 45.0%
GAGCCATGGTTGTGATCTTT+GGG - chr4.2:2722767-2722786 None:intergenic 45.0%
GATGCAACGTTGAAGGGTTA+AGG - chr4.2:2722664-2722683 None:intergenic 45.0%
GCGTTTCCGTTTGTTTGGAA+TGG + chr4.2:2723117-2723136 MS.gene049062:CDS 45.0%
GTCTTTCACTTCGGTTTCAC+CGG + chr4.2:2722939-2722958 MS.gene049062:CDS 45.0%
TACCAGTCAACAACGGTTTC+TGG - chr4.2:2723790-2723809 None:intergenic 45.0%
TCTCCACACAAGTAAGCAAG+TGG - chr4.2:2723733-2723752 None:intergenic 45.0%
TCTTTGGCACTTCCAATCCT+TGG + chr4.2:2723449-2723468 MS.gene049062:CDS 45.0%
TGAGCCATGGTTGTGATCTT+TGG - chr4.2:2722768-2722787 None:intergenic 45.0%
TGTTCCTCATCGCGTATGTT+TGG + chr4.2:2723003-2723022 MS.gene049062:CDS 45.0%
! ACTTGGTAACTGTCCTCAGA+CGG + chr4.2:2723481-2723500 MS.gene049062:CDS 45.0%
! TGGTTGTGATCTTTGGGTTC+TGG - chr4.2:2722761-2722780 None:intergenic 45.0%
! TTTAGGACGGTTTGCTCCAA+AGG - chr4.2:2722634-2722653 None:intergenic 45.0%
!!! CTTGCGTTTTGGTTTGACGT+TGG + chr4.2:2723602-2723621 MS.gene049062:CDS 45.0%
ACAGGGATGAGGTTTCGTTG+GGG + chr4.2:2723386-2723405 MS.gene049062:CDS 50.0%
CACAGGGATGAGGTTTCGTT+GGG + chr4.2:2723385-2723404 MS.gene049062:CDS 50.0%
CACCGTAACCGTTGCTTCTA+TGG + chr4.2:2723187-2723206 MS.gene049062:CDS 50.0%
CAGCGACGAATATTGCTACC+AGG - chr4.2:2723334-2723353 None:intergenic 50.0%
CATAGAAGCAACGGTTACGG+TGG - chr4.2:2723189-2723208 None:intergenic 50.0%
CCAAAGGCTTGTGAACAGAG+AGG - chr4.2:2722618-2722637 None:intergenic 50.0%
CCCCATAGAAGCAACGGTTA+CGG - chr4.2:2723192-2723211 None:intergenic 50.0%
CCGTAACCGTTGCTTCTATG+GGG + chr4.2:2723189-2723208 MS.gene049062:CDS 50.0%
CCTCTCTGTTCACAAGCCTT+TGG + chr4.2:2722615-2722634 MS.gene049062:CDS 50.0%
GAACCCAGCACAACAAACCT+GGG - chr4.2:2723660-2723679 None:intergenic 50.0%
GAGTGATTCCTTGTGCACGA+AGG - chr4.2:2722857-2722876 None:intergenic 50.0%
GGAAGAGGATGCAACGTTGA+AGG - chr4.2:2722671-2722690 None:intergenic 50.0%
GGAGAACTTGAACTTGCAGC+CGG + chr4.2:2722522-2722541 MS.gene049062:CDS 50.0%
GTAGAGAAATTCTCTCCGGC+TGG + chr4.2:2723063-2723082 MS.gene049062:CDS 50.0%
GTTTCCAACACGCGTGGAAA+CGG - chr4.2:2723270-2723289 None:intergenic 50.0%
TAGAAGCAACGGTTACGGTG+GGG - chr4.2:2723187-2723206 None:intergenic 50.0%
TGAACCCAGCACAACAAACC+TGG - chr4.2:2723661-2723680 None:intergenic 50.0%
TGCCCACTTGCTTACTTGTG+TGG + chr4.2:2723727-2723746 MS.gene049062:CDS 50.0%
TGGTTTGACGTTGGGTTTCG+CGG + chr4.2:2723611-2723630 MS.gene049062:CDS 50.0%
TTCCATTCCGAGAGAGAGAC+CGG - chr4.2:2722595-2722614 None:intergenic 50.0%
! CCGTTTGTTTGGAATGGTGG+TGG + chr4.2:2723123-2723142 MS.gene049062:CDS 50.0%
! CTATATGGTGGGGATGCCAA+TGG + chr4.2:2723571-2723590 MS.gene049062:CDS 50.0%
! GGTTCTGGTGCAACCAAATG+AGG - chr4.2:2722746-2722765 None:intergenic 50.0%
AACGCAAGCACAACCGCCAT+TGG - chr4.2:2723590-2723609 None:intergenic 55.0%
AACGGAGTAGCCGGTGATGT+TGG - chr4.2:2722568-2722587 None:intergenic 55.0%
AAGCTCGTTTCCAACACGCG+TGG - chr4.2:2723276-2723295 None:intergenic 55.0%
ACGCGTGTTGGAAACGAGCT+TGG + chr4.2:2723275-2723294 MS.gene049062:CDS 55.0%
ATCGCCTTCGCCAACATCAC+CGG + chr4.2:2722555-2722574 MS.gene049062:CDS 55.0%
CCAACGAAACCTCATCCCTG+TGG - chr4.2:2723387-2723406 None:intergenic 55.0%
CCACAGGGATGAGGTTTCGT+TGG + chr4.2:2723384-2723403 MS.gene049062:CDS 55.0%
GAGAGAGAGACCGGAGAGAA+CGG - chr4.2:2722586-2722605 None:intergenic 55.0%
GGCCAGAAACCGTTGTTGAC+TGG + chr4.2:2723785-2723804 MS.gene049062:CDS 55.0%
GGTAACTGTCCTCAGACGGT+GGG + chr4.2:2723485-2723504 MS.gene049062:CDS 55.0%
GGTTTGACGTTGGGTTTCGC+GGG + chr4.2:2723612-2723631 MS.gene049062:CDS 55.0%
GTGGCGGCGAATGTGAATCT+TGG + chr4.2:2723542-2723561 MS.gene049062:CDS 55.0%
GTGTTGTCAGGGGAACAGCA+AGG + chr4.2:2723513-2723532 MS.gene049062:CDS 55.0%
GTTCTCTCCGGTCTCTCTCT+CGG + chr4.2:2722585-2722604 MS.gene049062:CDS 55.0%
TCGGAGTAAAGGCTTCCAGC+CGG - chr4.2:2723081-2723100 None:intergenic 55.0%
TGGTAACTGTCCTCAGACGG+TGG + chr4.2:2723484-2723503 MS.gene049062:CDS 55.0%
TTAGCCGTTTCCACGCGTGT+TGG + chr4.2:2723263-2723282 MS.gene049062:CDS 55.0%
! CAGCGACGGTTTTTGCCACA+GGG + chr4.2:2723369-2723388 MS.gene049062:CDS 55.0%
! CGGTTTTTGCCACAGGGATG+AGG + chr4.2:2723375-2723394 MS.gene049062:CDS 55.0%
! GAATAGGTTGGAGGCTGCAG+AGG - chr4.2:2722976-2722995 None:intergenic 55.0%
! GCTTTGGTTGGGCTTGCTCT+CGG + chr4.2:2723634-2723653 MS.gene049062:CDS 55.0%
! TATGGTGGGGATGCCAATGG+CGG + chr4.2:2723574-2723593 MS.gene049062:CDS 55.0%
! TCAGCGACGGTTTTTGCCAC+AGG + chr4.2:2723368-2723387 MS.gene049062:CDS 55.0%
! TGGCGGTTGTGCTTGCGTTT+TGG + chr4.2:2723591-2723610 MS.gene049062:CDS 55.0%
AACAGCAAGGCCAGGTGTGG+CGG + chr4.2:2723526-2723545 MS.gene049062:CDS 60.0%
AACGGAAACGCAGCTTGGCG+TGG - chr4.2:2723108-2723127 None:intergenic 60.0%
ACCGGAGAGAACGGAGTAGC+CGG - chr4.2:2722577-2722596 None:intergenic 60.0%
ACCGGCTACTCCGTTCTCTC+CGG + chr4.2:2722573-2722592 MS.gene049062:CDS 60.0%
ACGTTGGGTTTCGCGGGCTT+TGG + chr4.2:2723618-2723637 MS.gene049062:CDS 60.0%
CTCCGGTCTCTCTCTCGGAA+TGG + chr4.2:2722590-2722609 MS.gene049062:CDS 60.0%
CTGGCATACACTGCGCCACA+TGG + chr4.2:2723030-2723049 MS.gene049062:CDS 60.0%
GAAGGCGATGGCGAGTGAAC+CGG - chr4.2:2722544-2722563 None:intergenic 60.0%
GGCGTGGCAAGTCGGAGTAA+AGG - chr4.2:2723092-2723111 None:intergenic 60.0%
GGTGATGTTGGCGAAGGCGA+TGG - chr4.2:2722556-2722575 None:intergenic 60.0%
GTAGCCGGTGATGTTGGCGA+AGG - chr4.2:2722562-2722581 None:intergenic 60.0%
TCTACTCGGCGCTGTCCATG+TGG - chr4.2:2723048-2723067 None:intergenic 60.0%
TTCACATTCGCCGCCACACC+TGG - chr4.2:2723539-2723558 None:intergenic 60.0%
! GGGTTTCGCGGGCTTTGGTT+GGG + chr4.2:2723623-2723642 MS.gene049062:CDS 60.0%
! TGGGTTTCGCGGGCTTTGGT+TGG + chr4.2:2723622-2723641 MS.gene049062:CDS 60.0%
!! CGGCCCAGGTTTGTTGTGCT+GGG + chr4.2:2723654-2723673 MS.gene049062:CDS 60.0%
!! GAGGAGAGTGCCAGCAAGTG+AGG - chr4.2:2722895-2722914 None:intergenic 60.0%
!! GGTGGGTTGCGGTGTTGTCA+GGG + chr4.2:2723502-2723521 MS.gene049062:CDS 60.0%
!! GTGGGTTGCGGTGTTGTCAG+GGG + chr4.2:2723503-2723522 MS.gene049062:CDS 60.0%
!! TCGGCCCAGGTTTGTTGTGC+TGG + chr4.2:2723653-2723672 MS.gene049062:CDS 60.0%
!! TGTCCTCAGACGGTGGGTTG+CGG + chr4.2:2723491-2723510 MS.gene049062:CDS 60.0%
ACACCGCAACCCACCGTCTG+AGG - chr4.2:2723497-2723516 None:intergenic 65.0%
CGCAGCTTGGCGTGGCAAGT+CGG - chr4.2:2723100-2723119 None:intergenic 65.0%
GGGAACAGCAAGGCCAGGTG+TGG + chr4.2:2723523-2723542 MS.gene049062:CDS 65.0%
GTCAGGGGAACAGCAAGGCC+AGG + chr4.2:2723518-2723537 MS.gene049062:CDS 65.0%
TGCAGAGGCAGCTGGGACTC+CGG - chr4.2:2722961-2722980 None:intergenic 65.0%
TGGAGGCTGCAGAGGCAGCT+GGG - chr4.2:2722968-2722987 None:intergenic 65.0%
TTGGAGGCTGCAGAGGCAGC+TGG - chr4.2:2722969-2722988 None:intergenic 65.0%
!! CGGTGGGTTGCGGTGTTGTC+AGG + chr4.2:2723501-2723520 MS.gene049062:CDS 65.0%
! GTTGGGCTTGCTCTCGGCCC+AGG + chr4.2:2723640-2723659 MS.gene049062:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 2722417 2723826 2722417 ID=MS.gene049062
chr4.2 mRNA 2722417 2723826 2722417 ID=MS.gene049062.t1;Parent=MS.gene049062
chr4.2 exon 2722417 2723826 2722417 ID=MS.gene049062.t1.exon1;Parent=MS.gene049062.t1
chr4.2 CDS 2722417 2723826 2722417 ID=cds.MS.gene049062.t1;Parent=MS.gene049062.t1
Gene Sequence

>MS.gene049062

ATGGAAGAAATGAAATCCCTAATGATGCTTGCTTTTCCAATAGCCATAACAGCTCTTATTTTCTATTCTCGTTCCATGGTGTCCATGATGTTACTAGGCTATCTAGGAGAACTTGAACTTGCAGCCGGTTCACTCGCCATCGCCTTCGCCAACATCACCGGCTACTCCGTTCTCTCCGGTCTCTCTCTCGGAATGGAACCTCTCTGTTCACAAGCCTTTGGAGCAAACCGTCCTAAACTCCTATCCTTAACCCTTCAACGTTGCATCCTCTTCCTGTTACTATGTTCCATTCCCATTTCCTTCATGTGGCTTAACATGTCACAAATCCTCATTTGGTTGCACCAGAACCCAAAGATCACAACCATGGCTCACACATATCTAATCTTCTCTCTCCCTGATCTCATCACAAATTCATTCATCCACCCTATACGTATTTACCTTCGTGCACAAGGAATCACTCACCCAATTACATTAGCCTCACTTGCTGGCACTCTCCTCCATCTTCCACTAAATTACTTCTTTGTCTTTCACTTCGGTTTCACCGGAGTCCCAGCTGCCTCTGCAGCCTCCAACCTATTCATTGTGTTGTTCCTCATCGCGTATGTTTGGTTAACTGGCATACACTGCGCCACATGGACAGCGCCGAGTAGAGAAATTCTCTCCGGCTGGAAGCCTTTACTCCGACTTGCCACGCCAAGCTGCGTTTCCGTTTGTTTGGAATGGTGGTGGTATGAAGTAATGATCATTTTATGTGGTTTACTTGTGGACCCCACCGTAACCGTTGCTTCTATGGGGATTTTAATTCAAACTACTGCATTCATTTATGTCTTTCCATCTTCACTTGGATTAGCCGTTTCCACGCGTGTTGGAAACGAGCTTGGTGCTAACCGTCCTTTCCAAGCTAAGTTATCATCCCTGGTAGCAATATTCGTCGCTGTAATGATAGGTTTTTCAGCGACGGTTTTTGCCACAGGGATGAGGTTTCGTTGGGGAAGAATGTTTACTGCTGATGAAAATATTCTTCGGCTGACTTCTTTGGCACTTCCAATCCTTGGATTGTGTGAACTTGGTAACTGTCCTCAGACGGTGGGTTGCGGTGTTGTCAGGGGAACAGCAAGGCCAGGTGTGGCGGCGAATGTGAATCTTGGTGCTTTCTATATGGTGGGGATGCCAATGGCGGTTGTGCTTGCGTTTTGGTTTGACGTTGGGTTTCGCGGGCTTTGGTTGGGCTTGCTCTCGGCCCAGGTTTGTTGTGCTGGGTTCATGTTGTATGTGATTGCGATCACTGATTGGGATTTTGAAGCTATGCGTGCCCACTTGCTTACTTGTGTGGAGAATGGAATGGGTAGTGATGATGGGATGATAAACGGCCAGAAACCGTTGTTGACTGGTATTGTTGTTGAGGCTTGA
Protein sequence

>MS.gene049062.t1

MEEMKSLMMLAFPIAITALIFYSRSMVSMMLLGYLGELELAAGSLAIAFANITGYSVLSGLSLGMEPLCSQAFGANRPKLLSLTLQRCILFLLLCSIPISFMWLNMSQILIWLHQNPKITTMAHTYLIFSLPDLITNSFIHPIRIYLRAQGITHPITLASLAGTLLHLPLNYFFVFHFGFTGVPAASAASNLFIVLFLIAYVWLTGIHCATWTAPSREILSGWKPLLRLATPSCVSVCLEWWWYEVMIILCGLLVDPTVTVASMGILIQTTAFIYVFPSSLGLAVSTRVGNELGANRPFQAKLSSLVAIFVAVMIGFSATVFATGMRFRWGRMFTADENILRLTSLALPILGLCELGNCPQTVGCGVVRGTARPGVAANVNLGAFYMVGMPMAVVLAFWFDVGFRGLWLGLLSAQVCCAGFMLYVIAITDWDFEAMRAHLLTCVENGMGSDDGMINGQKPLLTGIVVEA