AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05988

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05988.t1 MTR_8g102430 92.342 444 16 1 1 444 22 447 0.0 822
MS.gene05988.t1 MTR_6g088805 69.462 465 138 2 1 465 26 486 0.0 667
MS.gene05988.t1 MTR_5g067460 55.098 461 201 3 3 461 56 512 0.0 533
MS.gene05988.t1 MTR_3g111020 56.828 454 194 1 1 452 50 503 0.0 526
MS.gene05988.t1 MTR_1g101500 54.978 462 206 1 2 461 64 525 2.30e-179 513
MS.gene05988.t1 MTR_5g010830 55.631 444 195 1 1 442 64 507 4.14e-179 512
MS.gene05988.t1 MTR_0039s0080 55.217 460 202 2 2 459 38 495 1.43e-174 500
MS.gene05988.t1 MTR_6g043810 48.941 472 230 3 2 462 23 494 2.38e-159 461
MS.gene05988.t1 MTR_6g043810 48.941 472 230 3 2 462 33 504 3.21e-159 461
MS.gene05988.t1 MTR_4g027920 46.485 441 232 2 2 438 156 596 3.12e-141 419
MS.gene05988.t1 MTR_2g435930 43.284 469 242 3 1 451 18 480 2.21e-133 395
MS.gene05988.t1 MTR_1g108810 35.469 437 278 3 1 434 33 468 2.98e-84 268
MS.gene05988.t1 MTR_6g081400 35.294 442 281 3 2 439 15 455 1.08e-83 266
MS.gene05988.t1 MTR_6g081410 35.068 442 283 2 1 438 24 465 4.93e-83 264
MS.gene05988.t1 MTR_6g081780 34.389 442 286 2 1 438 24 465 2.53e-81 260
MS.gene05988.t1 MTR_6g066380 34.396 439 282 4 5 438 30 467 7.36e-80 256
MS.gene05988.t1 MTR_1g108840 35.648 432 260 7 2 418 41 469 1.41e-79 256
MS.gene05988.t1 MTR_5g011540 35.153 458 264 3 2 441 43 485 7.08e-79 254
MS.gene05988.t1 MTR_6g081810 32.877 438 270 3 1 438 26 439 5.81e-76 245
MS.gene05988.t1 MTR_6g081240 35.214 443 275 6 1 436 9 446 6.27e-75 242
MS.gene05988.t1 MTR_6g081300 34.240 441 286 2 1 438 9 448 8.12e-75 243
MS.gene05988.t1 MTR_6g081390 33.258 442 290 3 2 439 12 452 4.69e-73 238
MS.gene05988.t1 MTR_8g106670 32.054 443 291 3 3 439 13 451 3.04e-71 234
MS.gene05988.t1 MTR_7g082810 29.870 462 312 4 1 456 19 474 2.43e-69 229
MS.gene05988.t1 MTR_6g081310 35.431 429 261 4 25 438 19 446 7.27e-68 224
MS.gene05988.t1 MTR_2g078930 33.641 434 284 3 1 431 42 474 2.27e-66 221
MS.gene05988.t1 MTR_6g004220 30.856 444 300 4 1 439 22 463 3.38e-65 218
MS.gene05988.t1 MTR_2g079020 32.794 433 289 1 1 431 36 468 2.22e-63 213
MS.gene05988.t1 MTR_6g004220 30.752 439 297 4 1 434 29 465 2.29e-63 213
MS.gene05988.t1 MTR_2g078970 33.181 437 282 5 1 431 30 462 2.02e-62 211
MS.gene05988.t1 MTR_1g109060 29.638 442 305 4 2 439 24 463 4.09e-62 209
MS.gene05988.t1 MTR_4g048000 31.136 440 299 2 3 439 65 503 1.82e-61 209
MS.gene05988.t1 MTR_2g088400 30.594 438 302 2 3 438 53 490 3.38e-61 208
MS.gene05988.t1 MTR_2g078960 32.564 433 290 1 1 431 32 464 4.86e-61 207
MS.gene05988.t1 MTR_1g109060 29.519 437 302 4 2 434 24 458 6.80e-60 204
MS.gene05988.t1 MTR_1g108990 29.638 442 305 3 2 439 25 464 1.32e-59 203
MS.gene05988.t1 MTR_2g022980 28.474 439 312 2 3 439 49 487 3.09e-55 192
MS.gene05988.t1 MTR_2g088400 30.370 405 280 2 3 405 53 457 9.55e-54 187
MS.gene05988.t1 MTR_8g069470 30.112 445 300 5 1 439 49 488 3.48e-52 184
MS.gene05988.t1 MTR_5g033100 27.790 439 314 2 3 439 57 494 2.02e-49 177
MS.gene05988.t1 MTR_7g082800 29.353 402 263 7 65 455 5 396 4.16e-49 173
MS.gene05988.t1 MTR_5g032750 29.955 444 303 5 2 440 36 476 2.58e-48 173
MS.gene05988.t1 MTR_5g032720 28.668 443 308 5 3 440 37 476 9.77e-48 171
MS.gene05988.t1 MTR_8g063590 28.777 417 294 2 25 438 76 492 6.33e-47 170
MS.gene05988.t1 MTR_1g021740 27.335 439 316 2 3 439 36 473 1.29e-46 168
MS.gene05988.t1 MTR_6g004220 29.661 354 246 2 1 351 29 382 1.38e-46 167
MS.gene05988.t1 MTR_5g090290 28.507 442 306 4 3 438 47 484 9.41e-44 162
MS.gene05988.t1 MTR_3g099700 28.054 442 310 5 3 439 47 485 5.04e-40 150
MS.gene05988.t1 MTR_6g047610 27.765 443 305 9 2 437 31 465 3.85e-39 148
MS.gene05988.t1 MTR_1g100180 26.147 436 314 4 3 434 44 475 9.10e-39 147
MS.gene05988.t1 MTR_7g087400 25.806 465 319 6 2 451 30 483 1.88e-38 146
MS.gene05988.t1 MTR_3g099720 25.624 441 323 3 3 440 42 480 3.82e-37 142
MS.gene05988.t1 MTR_6g004220 30.034 293 200 3 1 290 29 319 7.35e-36 135
MS.gene05988.t1 MTR_6g047590 28.442 443 302 9 2 437 31 465 6.40e-35 136
MS.gene05988.t1 MTR_6g047640 26.862 443 291 9 2 437 31 447 5.99e-34 133
MS.gene05988.t1 MTR_7g087410 24.558 452 314 7 2 439 31 469 1.31e-33 132
MS.gene05988.t1 MTR_6g047640 27.540 443 307 8 2 437 31 466 1.61e-33 132
MS.gene05988.t1 MTR_5g087930 27.708 397 262 5 19 412 43 417 2.73e-33 131
MS.gene05988.t1 MTR_2g078990 31.164 292 191 5 146 431 4 291 3.09e-32 125
MS.gene05988.t1 MTR_7g087370 25.727 447 275 10 2 434 30 433 1.67e-31 126
MS.gene05988.t1 MTR_6g027190 25.330 454 308 7 2 439 24 462 9.32e-30 121
MS.gene05988.t1 MTR_7g087430 25.594 379 275 3 65 439 5 380 7.21e-29 117
MS.gene05988.t1 MTR_6g027200 25.053 471 320 8 2 453 36 492 4.71e-28 117
MS.gene05988.t1 MTR_7g087410 23.393 389 279 5 61 439 1 380 4.21e-27 112
MS.gene05988.t1 MTR_2g079030 32.642 193 130 0 239 431 319 511 3.31e-23 102
MS.gene05988.t1 MTR_5g090280 22.549 408 272 6 64 438 4 400 7.18e-15 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05988.t1 AT4G29140 65.328 473 153 3 1 462 54 526 0.0 636
MS.gene05988.t1 AT5G19700 65.247 446 153 1 2 445 35 480 0.0 615
MS.gene05988.t1 AT1G58340 57.684 449 188 1 1 447 56 504 1.00e-180 516
MS.gene05988.t1 AT5G52050 47.280 478 233 4 1 466 33 503 1.27e-161 467
MS.gene05988.t1 AT4G23030 54.167 456 206 2 1 453 30 485 1.26e-157 456
MS.gene05988.t1 AT1G71870 46.300 473 233 4 1 452 22 494 2.78e-154 448
MS.gene05988.t1 AT2G38510 48.156 461 235 3 2 458 5 465 1.78e-151 440
MS.gene05988.t1 AT5G49130 47.712 459 220 4 1 441 24 480 3.48e-140 412
MS.gene05988.t1 AT4G22790 36.674 469 273 5 1 452 28 489 2.94e-86 273
MS.gene05988.t1 AT5G52450 35.763 439 280 1 2 438 25 463 8.13e-80 256
MS.gene05988.t1 AT1G73700 35.526 456 280 4 2 452 23 469 1.47e-77 250
MS.gene05988.t1 AT2G34360 31.818 440 290 3 2 438 26 458 1.27e-69 229
MS.gene05988.t1 AT1G15150 30.682 440 301 3 3 439 31 469 8.34e-69 228
MS.gene05988.t1 AT1G71140 30.562 445 299 4 1 439 24 464 3.64e-68 226
MS.gene05988.t1 AT1G15170 31.818 440 296 3 3 439 34 472 1.08e-67 224
MS.gene05988.t1 AT1G66760 32.805 442 291 3 2 439 27 466 6.36e-67 222
MS.gene05988.t1 AT1G15160 30.000 440 304 3 3 439 31 469 8.30e-67 222
MS.gene05988.t1 AT1G66760 32.574 439 290 3 2 436 27 463 2.46e-65 218
MS.gene05988.t1 AT1G64820 29.932 441 307 1 1 439 27 467 6.02e-64 215
MS.gene05988.t1 AT1G66780 29.762 420 293 1 22 439 54 473 1.87e-62 211
MS.gene05988.t1 AT1G15180 31.973 441 294 5 3 439 35 473 1.04e-61 209
MS.gene05988.t1 AT2G04050 32.200 441 293 5 3 439 27 465 1.23e-59 203
MS.gene05988.t1 AT3G23550 30.415 434 298 3 1 431 29 461 1.99e-59 202
MS.gene05988.t1 AT1G61890 30.043 466 313 3 1 463 45 500 2.96e-58 200
MS.gene05988.t1 AT3G23560 29.724 434 301 3 1 431 37 469 5.55e-58 199
MS.gene05988.t1 AT2G34360 29.157 439 261 2 2 438 26 416 5.92e-58 197
MS.gene05988.t1 AT1G61890 30.664 437 299 2 1 434 45 480 1.51e-57 198
MS.gene05988.t1 AT1G11670 29.909 438 305 1 3 438 50 487 4.76e-57 197
MS.gene05988.t1 AT2G04040 29.613 439 307 1 3 439 27 465 4.91e-57 196
MS.gene05988.t1 AT4G21910 29.613 439 306 2 3 439 55 492 1.39e-56 196
MS.gene05988.t1 AT4G21910 29.613 439 306 2 3 439 55 492 1.39e-56 196
MS.gene05988.t1 AT4G21910 29.841 439 305 2 3 439 57 494 1.62e-56 196
MS.gene05988.t1 AT4G21910 29.841 439 305 2 3 439 57 494 1.62e-56 196
MS.gene05988.t1 AT2G04070 30.531 452 307 4 3 449 27 476 1.92e-56 194
MS.gene05988.t1 AT2G04080 30.820 451 305 6 3 448 27 475 7.29e-56 193
MS.gene05988.t1 AT3G26590 28.929 439 309 2 3 439 45 482 8.39e-56 193
MS.gene05988.t1 AT2G04100 28.733 442 307 3 3 439 30 468 3.24e-55 191
MS.gene05988.t1 AT2G04080 30.474 443 302 5 3 441 27 467 5.11e-54 188
MS.gene05988.t1 AT2G04100 28.604 437 304 3 3 434 30 463 1.29e-53 187
MS.gene05988.t1 AT2G04090 27.563 439 316 1 3 439 30 468 1.42e-53 187
MS.gene05988.t1 AT3G21690 28.311 438 312 2 3 438 52 489 1.11e-52 185
MS.gene05988.t1 AT5G38030 28.571 441 308 4 3 439 45 482 1.56e-52 184
MS.gene05988.t1 AT4G21903 29.369 412 288 2 30 439 80 490 1.76e-52 184
MS.gene05988.t1 AT1G15170 30.484 351 240 3 92 439 4 353 3.89e-52 180
MS.gene05988.t1 AT4G21903 29.584 409 285 2 30 436 80 487 4.72e-52 184
MS.gene05988.t1 AT1G61890 30.637 408 279 2 1 405 45 451 5.07e-51 180
MS.gene05988.t1 AT3G26590 29.365 378 264 2 64 439 4 380 9.49e-50 175
MS.gene05988.t1 AT4G00350 28.311 438 310 2 3 438 88 523 1.31e-49 177
MS.gene05988.t1 AT1G15150 29.630 351 243 3 92 439 4 353 3.19e-49 172
MS.gene05988.t1 AT1G66760 32.791 369 242 3 2 366 27 393 6.91e-49 172
MS.gene05988.t1 AT4G21903 29.443 377 264 1 65 439 5 381 1.10e-48 172
MS.gene05988.t1 AT1G23300 30.112 445 296 6 3 439 44 481 1.75e-48 174
MS.gene05988.t1 AT1G47530 28.442 443 308 6 2 439 34 472 9.64e-48 171
MS.gene05988.t1 AT3G23560 31.579 323 219 1 1 321 37 359 3.73e-47 168
MS.gene05988.t1 AT5G10420 28.345 441 312 3 3 440 38 477 7.85e-47 169
MS.gene05988.t1 AT3G59030 28.571 441 307 3 3 438 51 488 4.71e-46 167
MS.gene05988.t1 AT5G44050 28.507 442 310 4 3 440 40 479 1.75e-45 166
MS.gene05988.t1 AT1G12950 27.232 448 305 7 3 439 67 504 1.25e-44 164
MS.gene05988.t1 AT1G33090 28.341 434 304 3 3 432 36 466 2.34e-42 157
MS.gene05988.t1 AT1G33100 29.773 440 290 8 3 432 33 463 9.95e-42 155
MS.gene05988.t1 AT4G25640 28.111 434 308 2 3 434 34 465 1.74e-41 154
MS.gene05988.t1 AT4G25640 28.111 434 308 2 3 434 34 465 3.03e-41 154
MS.gene05988.t1 AT5G65380 27.045 440 315 4 3 438 37 474 4.43e-41 153
MS.gene05988.t1 AT1G66760 30.448 335 227 3 106 436 3 335 6.92e-41 149
MS.gene05988.t1 AT1G33110 27.500 440 300 8 3 432 36 466 2.74e-40 151
MS.gene05988.t1 AT1G33080 27.293 458 309 8 2 446 35 481 1.87e-38 146
MS.gene05988.t1 AT4G25640 28.141 398 282 2 3 398 34 429 1.90e-37 143
MS.gene05988.t1 AT1G47530 26.842 380 269 6 65 439 5 380 1.91e-37 141
MS.gene05988.t1 AT1G33080 26.923 442 306 5 2 434 35 468 3.30e-37 142
MS.gene05988.t1 AT3G03620 26.517 445 310 9 2 437 36 472 1.61e-34 135
MS.gene05988.t1 AT3G03620 26.517 445 310 9 2 437 36 472 1.61e-34 135
MS.gene05988.t1 AT3G59030 28.302 371 258 3 3 368 51 418 1.05e-33 132
MS.gene05988.t1 AT5G17700 26.622 447 311 10 2 439 33 471 1.87e-32 129
MS.gene05988.t1 AT5G17700 26.622 447 311 10 2 439 33 471 1.87e-32 129
MS.gene05988.t1 AT1G15180 30.827 266 178 5 92 353 4 267 1.55e-30 122
MS.gene05988.t1 AT1G33110 26.415 371 262 5 3 367 36 401 6.96e-28 115
MS.gene05988.t1 AT5G17700 26.087 322 225 8 2 316 33 348 6.99e-17 82.8

Find 165 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCGTAACCGTTGCTTCTA+TGG 0.153186 4.3:+3036297 MS.gene05988:CDS
GCAAGTGAGGCTAATGTAAT+TGG 0.227296 4.3:-3035989 None:intergenic
TGAGCCATGGTTGTGATCTT+TGG 0.229428 4.3:-3035875 None:intergenic
TCGGCCCAGGTTTGTTGTGC+TGG 0.242952 4.3:+3036763 MS.gene05988:CDS
ATCGCGTATGTTTGGTTAAC+TGG 0.250226 4.3:+3036121 MS.gene05988:CDS
CGGTGGGTTGCGGTGTTGTC+AGG 0.259072 4.3:+3036611 MS.gene05988:CDS
TATTCTTCGGCTGACTTCTT+TGG 0.260087 4.3:+3036543 MS.gene05988:CDS
TACCAGTCAACAACGGTTTC+TGG 0.265851 4.3:-3036897 None:intergenic
TGGTTGTGATCTTTGGGTTC+TGG 0.308597 4.3:-3035868 None:intergenic
TGGGTTTCGCGGGCTTTGGT+TGG 0.322674 4.3:+3036732 MS.gene05988:CDS
TTGGAAAAGCAAGCATCATT+AGG 0.333580 4.3:-3035544 None:intergenic
ATAAGAGCTGTTATGGCTAT+TGG 0.334848 4.3:-3035563 None:intergenic
GAGCCATGGTTGTGATCTTT+GGG 0.354470 4.3:-3035874 None:intergenic
AAGCTGCGTTTCCGTTTGTT+TGG 0.358580 4.3:+3036222 MS.gene05988:CDS
GAATCTTGGTGCTTTCTATA+TGG 0.360423 4.3:+3036666 MS.gene05988:CDS
AGGGTTAAGGATAGGAGTTT+AGG 0.362643 4.3:-3035758 None:intergenic
TGCAGAGGCAGCTGGGACTC+CGG 0.363910 4.3:-3036068 None:intergenic
TGGAAAAGCAAGCATCATTA+GGG 0.369962 4.3:-3035543 None:intergenic
ACATGTCACAAATCCTCATT+TGG 0.370537 4.3:+3035840 MS.gene05988:CDS
ATAGAAAATAAGAGCTGTTA+TGG 0.375099 4.3:-3035570 None:intergenic
TGGCGGTTGTGCTTGCGTTT+TGG 0.379139 4.3:+3036701 MS.gene05988:CDS
CACAGGGATGAGGTTTCGTT+GGG 0.383156 4.3:+3036495 MS.gene05988:CDS
CGGCCCAGGTTTGTTGTGCT+GGG 0.385823 4.3:+3036764 MS.gene05988:CDS
GTTGGGCTTGCTCTCGGCCC+AGG 0.386009 4.3:+3036750 MS.gene05988:CDS
TCTTTGGCACTTCCAATCCT+TGG 0.387985 4.3:+3036559 MS.gene05988:CDS
CCTCTCTGTTCACAAGCCTT+TGG 0.389078 4.3:+3035725 MS.gene05988:CDS
GTCTTTCACTTCGGTTTCAC+CGG 0.390820 4.3:+3036049 MS.gene05988:CDS
ACGTTGGGTTTCGCGGGCTT+TGG 0.391761 4.3:+3036728 MS.gene05988:CDS
ATTACATTAGCCTCACTTGC+TGG 0.391945 4.3:+3035992 MS.gene05988:CDS
GGGTTTCGCGGGCTTTGGTT+GGG 0.394583 4.3:+3036733 MS.gene05988:CDS
GTTAAGCCACATGAAGGAAA+TGG 0.397403 4.3:-3035819 None:intergenic
TTGGAGGCTGCAGAGGCAGC+TGG 0.401997 4.3:-3036076 None:intergenic
ATATTCGTCGCTGTAATGAT+AGG 0.402264 4.3:+3036451 MS.gene05988:CDS
TTTGGAATGGTGGTGGTATG+AGG 0.408884 4.3:+3036240 MS.gene05988:CDS
ACCGTAACCGTTGCTTCTAT+GGG 0.420509 4.3:+3036298 MS.gene05988:CDS
CTGCTGATGAAAATATTCTT+CGG 0.422541 4.3:+3036530 MS.gene05988:CDS
TGGGAATGGAACATAGTAAC+AGG 0.424607 4.3:-3035799 None:intergenic
AAGACAAAGAAGTAATTTAG+TGG 0.425047 4.3:-3036031 None:intergenic
CACGAAGGTAAATACGTATA+GGG 0.425365 4.3:-3035949 None:intergenic
CCACAGGGATGAGGTTTCGT+TGG 0.426710 4.3:+3036494 MS.gene05988:CDS
AATGAATTTGTGATGAGATC+AGG 0.427364 4.3:-3035920 None:intergenic
CTTGGTGCTTTCTATATGGT+GGG 0.430875 4.3:+3036670 MS.gene05988:CDS
GTTCTCTCCGGTCTCTCTCT+CGG 0.430975 4.3:+3035695 MS.gene05988:CDS
TGTTCCTCATCGCGTATGTT+TGG 0.431746 4.3:+3036113 MS.gene05988:CDS
GCACGAAGGTAAATACGTAT+AGG 0.432233 4.3:-3035950 None:intergenic
AGAAGTAATTTAGTGGAAGA+TGG 0.436636 4.3:-3036024 None:intergenic
GGTGGGTTGCGGTGTTGTCA+GGG 0.445474 4.3:+3036612 MS.gene05988:CDS
GATGCAACGTTGAAGGGTTA+AGG 0.451908 4.3:-3035771 None:intergenic
TGAACCCAGCACAACAAACC+TGG 0.453660 4.3:-3036768 None:intergenic
GAAGGCGATGGCGAGTGAAC+CGG 0.458073 4.3:-3035651 None:intergenic
ATGATGTTCCTAGGCTATCT+AGG 0.458663 4.3:+3035611 MS.gene05988:CDS
CCATTCCCATTTCCTTCATG+TGG 0.460327 4.3:+3035813 MS.gene05988:CDS
GCGTTTCCGTTTGTTTGGAA+TGG 0.460881 4.3:+3036227 MS.gene05988:CDS
TTGCTTACTTGTGTGGAGAA+TGG 0.463824 4.3:+3036844 MS.gene05988:CDS
TTCCATTCCGAGAGAGAGAC+CGG 0.465257 4.3:-3035702 None:intergenic
TTTAGGACGGTTTGCTCCAA+AGG 0.467132 4.3:-3035741 None:intergenic
TGGTTTGACGTTGGGTTTCG+CGG 0.472974 4.3:+3036721 MS.gene05988:CDS
GGTTCTGGTGCAACCAAATG+AGG 0.474606 4.3:-3035853 None:intergenic
ACCGGAGAGAACGGAGTAGC+CGG 0.474649 4.3:-3035684 None:intergenic
AATGGAATGGGTAGTGATGA+TGG 0.476279 4.3:+3036862 MS.gene05988:CDS
TTAAGCCACATGAAGGAAAT+GGG 0.477163 4.3:-3035818 None:intergenic
GCTTTGGTTGGGCTTGCTCT+CGG 0.477408 4.3:+3036744 MS.gene05988:CDS
TGAGGAACAACACAATGAAT+AGG 0.480105 4.3:-3036099 None:intergenic
TTCACATTCGCCGCCACACC+TGG 0.480133 4.3:-3036646 None:intergenic
AGTGATGATGGGATGATAAA+CGG 0.483280 4.3:+3036874 MS.gene05988:CDS
ATCGCCTTCGCCAACATCAC+CGG 0.484058 4.3:+3035665 MS.gene05988:CDS
CTCCGGTCTCTCTCTCGGAA+TGG 0.485898 4.3:+3035700 MS.gene05988:CDS
TGTCCTCAGACGGTGGGTTG+CGG 0.495604 4.3:+3036601 MS.gene05988:CDS
ACGCGTGTTGGAAACGAGCT+TGG 0.496589 4.3:+3036385 MS.gene05988:CDS
GTTTCCAACACGCGTGGAAA+CGG 0.498770 4.3:-3036377 None:intergenic
TATGTCTTTCCATCTTCACT+TGG 0.500525 4.3:+3036349 MS.gene05988:CDS
ATGTGATTGCGATCACTGAT+TGG 0.500968 4.3:+3036797 MS.gene05988:CDS
CCAAGCTAAGTTATCATCCC+TGG 0.503107 4.3:+3036423 MS.gene05988:CDS
CAAGTGAGGCTAATGTAATT+GGG 0.503400 4.3:-3035988 None:intergenic
GGAAGAGGATGCAACGTTGA+AGG 0.504510 4.3:-3035778 None:intergenic
CCCCATAGAAGCAACGGTTA+CGG 0.505664 4.3:-3036299 None:intergenic
CCAGGGATGATAACTTAGCT+TGG 0.506191 4.3:-3036423 None:intergenic
GTAGAGAAATTCTCTCCGGC+TGG 0.506195 4.3:+3036173 MS.gene05988:CDS
GGTTTGACGTTGGGTTTCGC+GGG 0.507344 4.3:+3036722 MS.gene05988:CDS
AACAGCAAGGCCAGGTGTGG+CGG 0.508991 4.3:+3036636 MS.gene05988:CDS
ACGGCTAATCCAAGTGAAGA+TGG 0.509085 4.3:-3036358 None:intergenic
TGTGATTGCGATCACTGATT+GGG 0.512724 4.3:+3036798 MS.gene05988:CDS
ATCCTTGGATTGTGTGAACT+TGG 0.512999 4.3:+3036574 MS.gene05988:CDS
ACCGGCTACTCCGTTCTCTC+CGG 0.514145 4.3:+3035683 MS.gene05988:CDS
ATGGTGTCCATGATGTTCCT+AGG 0.514854 4.3:+3035602 MS.gene05988:CDS
CCACCACCATTCCAAACAAA+CGG 0.515374 4.3:-3036233 None:intergenic
TACTTCTTTGTCTTTCACTT+CGG 0.515725 4.3:+3036040 MS.gene05988:CDS
CGCAGCTTGGCGTGGCAAGT+CGG 0.517568 4.3:-3036207 None:intergenic
GTCAGGGGAACAGCAAGGCC+AGG 0.521102 4.3:+3036628 MS.gene05988:CDS
AACGCAAGCACAACCGCCAT+TGG 0.524005 4.3:-3036697 None:intergenic
TTTCCGTTTGTTTGGAATGG+TGG 0.524547 4.3:+3036230 MS.gene05988:CDS
CCGTTTGTTTGGAATGGTGG+TGG 0.525312 4.3:+3036233 MS.gene05988:CDS
GAGAGAGAGACCGGAGAGAA+CGG 0.527431 4.3:-3035693 None:intergenic
TCTTGGTGCTTTCTATATGG+TGG 0.529459 4.3:+3036669 MS.gene05988:CDS
TGGAGGCTGCAGAGGCAGCT+GGG 0.530384 4.3:-3036075 None:intergenic
GAGGAGAGTGCCAGCAAGTG+AGG 0.531668 4.3:-3036002 None:intergenic
AACGGAGTAGCCGGTGATGT+TGG 0.532013 4.3:-3035675 None:intergenic
TGGAACATAGTAACAGGAAG+AGG 0.532090 4.3:-3035793 None:intergenic
GGCCAGAAACCGTTGTTGAC+TGG 0.532184 4.3:+3036895 MS.gene05988:CDS
AGTTCACACAATCCAAGGAT+TGG 0.532587 4.3:-3036571 None:intergenic
TGACATGTTAAGCCACATGA+AGG 0.533931 4.3:-3035825 None:intergenic
CAGCGACGAATATTGCTACC+AGG 0.535583 4.3:-3036441 None:intergenic
GGCGTGGCAAGTCGGAGTAA+AGG 0.536544 4.3:-3036199 None:intergenic
CGTATTTACCTTCGTGCACA+AGG 0.537215 4.3:+3035956 MS.gene05988:CDS
GGTGATGTTGGCGAAGGCGA+TGG 0.540239 4.3:-3035663 None:intergenic
TAGATAGCCTAGGAACATCA+TGG 0.544584 4.3:-3035609 None:intergenic
GAAGAGGATGCAACGTTGAA+GGG 0.547608 4.3:-3035777 None:intergenic
CTATATGGTGGGGATGCCAA+TGG 0.550956 4.3:+3036681 MS.gene05988:CDS
TACCAAGTTCACACAATCCA+AGG 0.551400 4.3:-3036576 None:intergenic
GGGAACAGCAAGGCCAGGTG+TGG 0.554355 4.3:+3036633 MS.gene05988:CDS
GAACAACACAATGAATAGGT+TGG 0.554841 4.3:-3036095 None:intergenic
CCACATGAAGGAAATGGGAA+TGG 0.555412 4.3:-3035813 None:intergenic
GGAGAACTTGAACTTGCAGC+CGG 0.559934 4.3:+3035632 MS.gene05988:CDS
ATGAATTTGTGATGAGATCA+GGG 0.560522 4.3:-3035919 None:intergenic
TGCCCACTTGCTTACTTGTG+TGG 0.561216 4.3:+3036837 MS.gene05988:CDS
TGGTAACTGTCCTCAGACGG+TGG 0.568794 4.3:+3036594 MS.gene05988:CDS
ACACCGCAACCCACCGTCTG+AGG 0.569573 4.3:-3036604 None:intergenic
AGTAATTTAGTGGAAGATGG+AGG 0.572015 4.3:-3036021 None:intergenic
GAATAGGTTGGAGGCTGCAG+AGG 0.573910 4.3:-3036083 None:intergenic
CTGGCATACACTGCGCCACA+TGG 0.574182 4.3:+3036140 MS.gene05988:CDS
TTAGCCGTTTCCACGCGTGT+TGG 0.576266 4.3:+3036373 MS.gene05988:CDS
CAAGTTCTCCTAGATAGCCT+AGG 0.576301 4.3:-3035619 None:intergenic
AACGTTGAAGGGTTAAGGAT+AGG 0.580029 4.3:-3035766 None:intergenic
GTGGCGGCGAATGTGAATCT+TGG 0.580181 4.3:+3036652 MS.gene05988:CDS
TAAAATCCCCATAGAAGCAA+CGG 0.581538 4.3:-3036305 None:intergenic
GTGGGTTGCGGTGTTGTCAG+GGG 0.581939 4.3:+3036613 MS.gene05988:CDS
ATAACTTAGCTTGGAAAGGA+CGG 0.583020 4.3:-3036414 None:intergenic
ATGGAATGGGTAGTGATGAT+GGG 0.586061 4.3:+3036863 MS.gene05988:CDS
AACGGAAACGCAGCTTGGCG+TGG 0.586430 4.3:-3036215 None:intergenic
GTTGACTGGTATTGTTGTTG+AGG 0.589869 4.3:+3036909 MS.gene05988:CDS
AAACAAACGGAAACGCAGCT+TGG 0.594222 4.3:-3036220 None:intergenic
GATGATAACTTAGCTTGGAA+AGG 0.597780 4.3:-3036418 None:intergenic
ATAGAAGCAACGGTTACGGT+GGG 0.602058 4.3:-3036295 None:intergenic
ACTTGTGTGGAGAATGGAAT+GGG 0.602809 4.3:+3036850 MS.gene05988:CDS
TTAAGGATAGGAGTTTAGGA+CGG 0.604091 4.3:-3035754 None:intergenic
TCGGAGTAAAGGCTTCCAGC+CGG 0.605342 4.3:-3036188 None:intergenic
CAACACAATGAATAGGTTGG+AGG 0.605721 4.3:-3036092 None:intergenic
CCGGAGAGAATTTCTCTACT+CGG 0.606415 4.3:-3036169 None:intergenic
CCGTAACCGTTGCTTCTATG+GGG 0.611433 4.3:+3036299 MS.gene05988:CDS
CATAGAAGCAACGGTTACGG+TGG 0.617561 4.3:-3036296 None:intergenic
TCTCCACACAAGTAAGCAAG+TGG 0.626487 4.3:-3036840 None:intergenic
GTAGCCGGTGATGTTGGCGA+AGG 0.633540 4.3:-3035669 None:intergenic
GAACCCAGCACAACAAACCT+GGG 0.635945 4.3:-3036767 None:intergenic
GGTAACTGTCCTCAGACGGT+GGG 0.641343 4.3:+3036595 MS.gene05988:CDS
CCGAGTAGAGAAATTCTCTC+CGG 0.642731 4.3:+3036169 MS.gene05988:CDS
TTGGTGCTTTCTATATGGTG+GGG 0.643884 4.3:+3036671 MS.gene05988:CDS
TAGGAACATCATGGACACCA+TGG 0.647534 4.3:-3035600 None:intergenic
ACTTGGTAACTGTCCTCAGA+CGG 0.655240 4.3:+3036591 MS.gene05988:CDS
TCTACTCGGCGCTGTCCATG+TGG 0.656110 4.3:-3036155 None:intergenic
AGCGACGAATATTGCTACCA+GGG 0.668909 4.3:-3036440 None:intergenic
TACTTGTGTGGAGAATGGAA+TGG 0.668929 4.3:+3036849 MS.gene05988:CDS
GAAGGTAAATACGTATAGGG+TGG 0.671979 4.3:-3035946 None:intergenic
TTAACCAAACATACGCGATG+AGG 0.672780 4.3:-3036117 None:intergenic
TATGGTGGGGATGCCAATGG+CGG 0.673877 4.3:+3036684 MS.gene05988:CDS
AAGCTCGTTTCCAACACGCG+TGG 0.684465 4.3:-3036383 None:intergenic
GTGTTGTCAGGGGAACAGCA+AGG 0.686076 4.3:+3036623 MS.gene05988:CDS
GAACCCAAAGATCACAACCA+TGG 0.692294 4.3:+3035871 MS.gene05988:CDS
CTCCACACAAGTAAGCAAGT+GGG 0.696610 4.3:-3036839 None:intergenic
GATTAGATATGTGTGAGCCA+TGG 0.701135 4.3:-3035888 None:intergenic
CCAAAGGCTTGTGAACAGAG+AGG 0.712249 4.3:-3035725 None:intergenic
ACAGGGATGAGGTTTCGTTG+GGG 0.713111 4.3:+3036496 MS.gene05988:CDS
TAGAAGCAACGGTTACGGTG+GGG 0.713451 4.3:-3036294 None:intergenic
GAGTGATTCCTTGTGCACGA+AGG 0.717544 4.3:-3035964 None:intergenic
ACAACAATACCAGTCAACAA+CGG 0.720742 4.3:-3036904 None:intergenic
CCAACGAAACCTCATCCCTG+TGG 0.753980 4.3:-3036494 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGACAAAGAAGTAATTTAG+TGG - chr4.3:3036034-3036053 None:intergenic 25.0%
! ATAGAAAATAAGAGCTGTTA+TGG - chr4.3:3035573-3035592 None:intergenic 25.0%
AATGAATTTGTGATGAGATC+AGG - chr4.3:3035923-3035942 None:intergenic 30.0%
AGAAGTAATTTAGTGGAAGA+TGG - chr4.3:3036027-3036046 None:intergenic 30.0%
ATGAATTTGTGATGAGATCA+GGG - chr4.3:3035922-3035941 None:intergenic 30.0%
CTGCTGATGAAAATATTCTT+CGG + chr4.3:3036530-3036549 MS.gene05988:CDS 30.0%
TACTTCTTTGTCTTTCACTT+CGG + chr4.3:3036040-3036059 MS.gene05988:CDS 30.0%
! CATTTTATGTGGTTTACTTG+TGG + chr4.3:3036270-3036289 MS.gene05988:CDS 30.0%
! GAGGTAATGATCATTTTATG+TGG + chr4.3:3036259-3036278 MS.gene05988:CDS 30.0%
! TATTTTCTATTCTCGTTCCA+TGG + chr4.3:3035583-3035602 MS.gene05988:CDS 30.0%
ACAACAATACCAGTCAACAA+CGG - chr4.3:3036907-3036926 None:intergenic 35.0%
ACATGTCACAAATCCTCATT+TGG + chr4.3:3035840-3035859 MS.gene05988:CDS 35.0%
AGTAATTTAGTGGAAGATGG+AGG - chr4.3:3036024-3036043 None:intergenic 35.0%
ATAAGAGCTGTTATGGCTAT+TGG - chr4.3:3035566-3035585 None:intergenic 35.0%
ATATTCGTCGCTGTAATGAT+AGG + chr4.3:3036451-3036470 MS.gene05988:CDS 35.0%
CAAGTGAGGCTAATGTAATT+GGG - chr4.3:3035991-3036010 None:intergenic 35.0%
CACGAAGGTAAATACGTATA+GGG - chr4.3:3035952-3035971 None:intergenic 35.0%
GAACAACACAATGAATAGGT+TGG - chr4.3:3036098-3036117 None:intergenic 35.0%
TAAAATCCCCATAGAAGCAA+CGG - chr4.3:3036308-3036327 None:intergenic 35.0%
TATGTCTTTCCATCTTCACT+TGG + chr4.3:3036349-3036368 MS.gene05988:CDS 35.0%
TGAGGAACAACACAATGAAT+AGG - chr4.3:3036102-3036121 None:intergenic 35.0%
TGGAAAAGCAAGCATCATTA+GGG - chr4.3:3035546-3035565 None:intergenic 35.0%
TTAAGCCACATGAAGGAAAT+GGG - chr4.3:3035821-3035840 None:intergenic 35.0%
TTAAGGATAGGAGTTTAGGA+CGG - chr4.3:3035757-3035776 None:intergenic 35.0%
TTGGAAAAGCAAGCATCATT+AGG - chr4.3:3035547-3035566 None:intergenic 35.0%
! ATAACTTAGCTTGGAAAGGA+CGG - chr4.3:3036417-3036436 None:intergenic 35.0%
! GATGATAACTTAGCTTGGAA+AGG - chr4.3:3036421-3036440 None:intergenic 35.0%
!! AATGATAGGTTTTTCAGCGA+CGG + chr4.3:3036465-3036484 MS.gene05988:CDS 35.0%
!! AGTGATGATGGGATGATAAA+CGG + chr4.3:3036874-3036893 MS.gene05988:CDS 35.0%
!! GAATCTTGGTGCTTTCTATA+TGG + chr4.3:3036666-3036685 MS.gene05988:CDS 35.0%
AACGTTGAAGGGTTAAGGAT+AGG - chr4.3:3035769-3035788 None:intergenic 40.0%
AATGGAATGGGTAGTGATGA+TGG + chr4.3:3036862-3036881 MS.gene05988:CDS 40.0%
ACTTGTGTGGAGAATGGAAT+GGG + chr4.3:3036850-3036869 MS.gene05988:CDS 40.0%
AGGGTTAAGGATAGGAGTTT+AGG - chr4.3:3035761-3035780 None:intergenic 40.0%
AGTTCACACAATCCAAGGAT+TGG - chr4.3:3036574-3036593 None:intergenic 40.0%
ATCCTTGGATTGTGTGAACT+TGG + chr4.3:3036574-3036593 MS.gene05988:CDS 40.0%
ATCGCGTATGTTTGGTTAAC+TGG + chr4.3:3036121-3036140 MS.gene05988:CDS 40.0%
ATGATGTTCCTAGGCTATCT+AGG + chr4.3:3035611-3035630 MS.gene05988:CDS 40.0%
ATGGAATGGGTAGTGATGAT+GGG + chr4.3:3036863-3036882 MS.gene05988:CDS 40.0%
ATGTGATTGCGATCACTGAT+TGG + chr4.3:3036797-3036816 MS.gene05988:CDS 40.0%
GAAGGTAAATACGTATAGGG+TGG - chr4.3:3035949-3035968 None:intergenic 40.0%
GATTAGATATGTGTGAGCCA+TGG - chr4.3:3035891-3035910 None:intergenic 40.0%
GCAAGTGAGGCTAATGTAAT+TGG - chr4.3:3035992-3036011 None:intergenic 40.0%
GCACGAAGGTAAATACGTAT+AGG - chr4.3:3035953-3035972 None:intergenic 40.0%
GTTAAGCCACATGAAGGAAA+TGG - chr4.3:3035822-3035841 None:intergenic 40.0%
TACCAAGTTCACACAATCCA+AGG - chr4.3:3036579-3036598 None:intergenic 40.0%
TACTTGTGTGGAGAATGGAA+TGG + chr4.3:3036849-3036868 MS.gene05988:CDS 40.0%
TAGATAGCCTAGGAACATCA+TGG - chr4.3:3035612-3035631 None:intergenic 40.0%
TGACATGTTAAGCCACATGA+AGG - chr4.3:3035828-3035847 None:intergenic 40.0%
TGGAACATAGTAACAGGAAG+AGG - chr4.3:3035796-3035815 None:intergenic 40.0%
TGGGAATGGAACATAGTAAC+AGG - chr4.3:3035802-3035821 None:intergenic 40.0%
TTAACCAAACATACGCGATG+AGG - chr4.3:3036120-3036139 None:intergenic 40.0%
TTGCTTACTTGTGTGGAGAA+TGG + chr4.3:3036844-3036863 MS.gene05988:CDS 40.0%
! ATTACATTAGCCTCACTTGC+TGG + chr4.3:3035992-3036011 MS.gene05988:CDS 40.0%
! CAACACAATGAATAGGTTGG+AGG - chr4.3:3036095-3036114 None:intergenic 40.0%
! GTTGACTGGTATTGTTGTTG+AGG + chr4.3:3036909-3036928 MS.gene05988:CDS 40.0%
! TATTCTTCGGCTGACTTCTT+TGG + chr4.3:3036543-3036562 MS.gene05988:CDS 40.0%
! TGTGATTGCGATCACTGATT+GGG + chr4.3:3036798-3036817 MS.gene05988:CDS 40.0%
! TTGGTGCTTTCTATATGGTG+GGG + chr4.3:3036671-3036690 MS.gene05988:CDS 40.0%
! TTTCCGTTTGTTTGGAATGG+TGG + chr4.3:3036230-3036249 MS.gene05988:CDS 40.0%
!! CTTGGTGCTTTCTATATGGT+GGG + chr4.3:3036670-3036689 MS.gene05988:CDS 40.0%
!! TCTTGGTGCTTTCTATATGG+TGG + chr4.3:3036669-3036688 MS.gene05988:CDS 40.0%
!!! TTGCGTTTTGGTTTGACGTT+GGG + chr4.3:3036713-3036732 MS.gene05988:CDS 40.0%
AAACAAACGGAAACGCAGCT+TGG - chr4.3:3036223-3036242 None:intergenic 45.0%
AAGCTGCGTTTCCGTTTGTT+TGG + chr4.3:3036222-3036241 MS.gene05988:CDS 45.0%
ACCGTAACCGTTGCTTCTAT+GGG + chr4.3:3036298-3036317 MS.gene05988:CDS 45.0%
ACGGCTAATCCAAGTGAAGA+TGG - chr4.3:3036361-3036380 None:intergenic 45.0%
AGCGACGAATATTGCTACCA+GGG - chr4.3:3036443-3036462 None:intergenic 45.0%
ATAGAAGCAACGGTTACGGT+GGG - chr4.3:3036298-3036317 None:intergenic 45.0%
ATGGTGTCCATGATGTTCCT+AGG + chr4.3:3035602-3035621 MS.gene05988:CDS 45.0%
CAAGTTCTCCTAGATAGCCT+AGG - chr4.3:3035622-3035641 None:intergenic 45.0%
CCAAGCTAAGTTATCATCCC+TGG + chr4.3:3036423-3036442 MS.gene05988:CDS 45.0%
CCACATGAAGGAAATGGGAA+TGG - chr4.3:3035816-3035835 None:intergenic 45.0%
CCACCACCATTCCAAACAAA+CGG - chr4.3:3036236-3036255 None:intergenic 45.0%
CCAGGGATGATAACTTAGCT+TGG - chr4.3:3036426-3036445 None:intergenic 45.0%
CCATTCCCATTTCCTTCATG+TGG + chr4.3:3035813-3035832 MS.gene05988:CDS 45.0%
CCGAGTAGAGAAATTCTCTC+CGG + chr4.3:3036169-3036188 MS.gene05988:CDS 45.0%
CCGGAGAGAATTTCTCTACT+CGG - chr4.3:3036172-3036191 None:intergenic 45.0%
CGTATTTACCTTCGTGCACA+AGG + chr4.3:3035956-3035975 MS.gene05988:CDS 45.0%
CTCCACACAAGTAAGCAAGT+GGG - chr4.3:3036842-3036861 None:intergenic 45.0%
GAACCCAAAGATCACAACCA+TGG + chr4.3:3035871-3035890 MS.gene05988:CDS 45.0%
GAAGAGGATGCAACGTTGAA+GGG - chr4.3:3035780-3035799 None:intergenic 45.0%
GAGCCATGGTTGTGATCTTT+GGG - chr4.3:3035877-3035896 None:intergenic 45.0%
GATGCAACGTTGAAGGGTTA+AGG - chr4.3:3035774-3035793 None:intergenic 45.0%
GCGTTTCCGTTTGTTTGGAA+TGG + chr4.3:3036227-3036246 MS.gene05988:CDS 45.0%
GTCTTTCACTTCGGTTTCAC+CGG + chr4.3:3036049-3036068 MS.gene05988:CDS 45.0%
TACCAGTCAACAACGGTTTC+TGG - chr4.3:3036900-3036919 None:intergenic 45.0%
TAGGAACATCATGGACACCA+TGG - chr4.3:3035603-3035622 None:intergenic 45.0%
TCTCCACACAAGTAAGCAAG+TGG - chr4.3:3036843-3036862 None:intergenic 45.0%
TCTTTGGCACTTCCAATCCT+TGG + chr4.3:3036559-3036578 MS.gene05988:CDS 45.0%
TGAGCCATGGTTGTGATCTT+TGG - chr4.3:3035878-3035897 None:intergenic 45.0%
TGTTCCTCATCGCGTATGTT+TGG + chr4.3:3036113-3036132 MS.gene05988:CDS 45.0%
! ACTTGGTAACTGTCCTCAGA+CGG + chr4.3:3036591-3036610 MS.gene05988:CDS 45.0%
! TGGTTGTGATCTTTGGGTTC+TGG - chr4.3:3035871-3035890 None:intergenic 45.0%
! TTTAGGACGGTTTGCTCCAA+AGG - chr4.3:3035744-3035763 None:intergenic 45.0%
!! TTTGGAATGGTGGTGGTATG+AGG + chr4.3:3036240-3036259 MS.gene05988:CDS 45.0%
!!! CTTGCGTTTTGGTTTGACGT+TGG + chr4.3:3036712-3036731 MS.gene05988:CDS 45.0%
ACAGGGATGAGGTTTCGTTG+GGG + chr4.3:3036496-3036515 MS.gene05988:CDS 50.0%
CACAGGGATGAGGTTTCGTT+GGG + chr4.3:3036495-3036514 MS.gene05988:CDS 50.0%
CACCGTAACCGTTGCTTCTA+TGG + chr4.3:3036297-3036316 MS.gene05988:CDS 50.0%
CAGCGACGAATATTGCTACC+AGG - chr4.3:3036444-3036463 None:intergenic 50.0%
CATAGAAGCAACGGTTACGG+TGG - chr4.3:3036299-3036318 None:intergenic 50.0%
CCAAAGGCTTGTGAACAGAG+AGG - chr4.3:3035728-3035747 None:intergenic 50.0%
CCCCATAGAAGCAACGGTTA+CGG - chr4.3:3036302-3036321 None:intergenic 50.0%
CCGTAACCGTTGCTTCTATG+GGG + chr4.3:3036299-3036318 MS.gene05988:CDS 50.0%
CCTCTCTGTTCACAAGCCTT+TGG + chr4.3:3035725-3035744 MS.gene05988:CDS 50.0%
GAACCCAGCACAACAAACCT+GGG - chr4.3:3036770-3036789 None:intergenic 50.0%
GAGTGATTCCTTGTGCACGA+AGG - chr4.3:3035967-3035986 None:intergenic 50.0%
GGAAGAGGATGCAACGTTGA+AGG - chr4.3:3035781-3035800 None:intergenic 50.0%
GGAGAACTTGAACTTGCAGC+CGG + chr4.3:3035632-3035651 MS.gene05988:CDS 50.0%
GTAGAGAAATTCTCTCCGGC+TGG + chr4.3:3036173-3036192 MS.gene05988:CDS 50.0%
GTTTCCAACACGCGTGGAAA+CGG - chr4.3:3036380-3036399 None:intergenic 50.0%
TAGAAGCAACGGTTACGGTG+GGG - chr4.3:3036297-3036316 None:intergenic 50.0%
TGAACCCAGCACAACAAACC+TGG - chr4.3:3036771-3036790 None:intergenic 50.0%
TGCCCACTTGCTTACTTGTG+TGG + chr4.3:3036837-3036856 MS.gene05988:CDS 50.0%
TGGTTTGACGTTGGGTTTCG+CGG + chr4.3:3036721-3036740 MS.gene05988:CDS 50.0%
TTCCATTCCGAGAGAGAGAC+CGG - chr4.3:3035705-3035724 None:intergenic 50.0%
! CCGTTTGTTTGGAATGGTGG+TGG + chr4.3:3036233-3036252 MS.gene05988:CDS 50.0%
! CTATATGGTGGGGATGCCAA+TGG + chr4.3:3036681-3036700 MS.gene05988:CDS 50.0%
! GGTTCTGGTGCAACCAAATG+AGG - chr4.3:3035856-3035875 None:intergenic 50.0%
AACGCAAGCACAACCGCCAT+TGG - chr4.3:3036700-3036719 None:intergenic 55.0%
AACGGAGTAGCCGGTGATGT+TGG - chr4.3:3035678-3035697 None:intergenic 55.0%
AAGCTCGTTTCCAACACGCG+TGG - chr4.3:3036386-3036405 None:intergenic 55.0%
ACGCGTGTTGGAAACGAGCT+TGG + chr4.3:3036385-3036404 MS.gene05988:CDS 55.0%
ATCGCCTTCGCCAACATCAC+CGG + chr4.3:3035665-3035684 MS.gene05988:CDS 55.0%
CCAACGAAACCTCATCCCTG+TGG - chr4.3:3036497-3036516 None:intergenic 55.0%
CCACAGGGATGAGGTTTCGT+TGG + chr4.3:3036494-3036513 MS.gene05988:CDS 55.0%
GAGAGAGAGACCGGAGAGAA+CGG - chr4.3:3035696-3035715 None:intergenic 55.0%
GGCCAGAAACCGTTGTTGAC+TGG + chr4.3:3036895-3036914 MS.gene05988:CDS 55.0%
GGTAACTGTCCTCAGACGGT+GGG + chr4.3:3036595-3036614 MS.gene05988:CDS 55.0%
GGTTTGACGTTGGGTTTCGC+GGG + chr4.3:3036722-3036741 MS.gene05988:CDS 55.0%
GTGGCGGCGAATGTGAATCT+TGG + chr4.3:3036652-3036671 MS.gene05988:CDS 55.0%
GTGTTGTCAGGGGAACAGCA+AGG + chr4.3:3036623-3036642 MS.gene05988:CDS 55.0%
GTTCTCTCCGGTCTCTCTCT+CGG + chr4.3:3035695-3035714 MS.gene05988:CDS 55.0%
TCGGAGTAAAGGCTTCCAGC+CGG - chr4.3:3036191-3036210 None:intergenic 55.0%
TGGTAACTGTCCTCAGACGG+TGG + chr4.3:3036594-3036613 MS.gene05988:CDS 55.0%
TTAGCCGTTTCCACGCGTGT+TGG + chr4.3:3036373-3036392 MS.gene05988:CDS 55.0%
! CAGCGACGGTTTTTGCCACA+GGG + chr4.3:3036479-3036498 MS.gene05988:CDS 55.0%
! CGGTTTTTGCCACAGGGATG+AGG + chr4.3:3036485-3036504 MS.gene05988:CDS 55.0%
! GAATAGGTTGGAGGCTGCAG+AGG - chr4.3:3036086-3036105 None:intergenic 55.0%
! GCTTTGGTTGGGCTTGCTCT+CGG + chr4.3:3036744-3036763 MS.gene05988:CDS 55.0%
! TATGGTGGGGATGCCAATGG+CGG + chr4.3:3036684-3036703 MS.gene05988:CDS 55.0%
! TCAGCGACGGTTTTTGCCAC+AGG + chr4.3:3036478-3036497 MS.gene05988:CDS 55.0%
! TGGCGGTTGTGCTTGCGTTT+TGG + chr4.3:3036701-3036720 MS.gene05988:CDS 55.0%
AACAGCAAGGCCAGGTGTGG+CGG + chr4.3:3036636-3036655 MS.gene05988:CDS 60.0%
AACGGAAACGCAGCTTGGCG+TGG - chr4.3:3036218-3036237 None:intergenic 60.0%
ACCGGAGAGAACGGAGTAGC+CGG - chr4.3:3035687-3035706 None:intergenic 60.0%
ACCGGCTACTCCGTTCTCTC+CGG + chr4.3:3035683-3035702 MS.gene05988:CDS 60.0%
ACGTTGGGTTTCGCGGGCTT+TGG + chr4.3:3036728-3036747 MS.gene05988:CDS 60.0%
CTCCGGTCTCTCTCTCGGAA+TGG + chr4.3:3035700-3035719 MS.gene05988:CDS 60.0%
CTGGCATACACTGCGCCACA+TGG + chr4.3:3036140-3036159 MS.gene05988:CDS 60.0%
GAAGGCGATGGCGAGTGAAC+CGG - chr4.3:3035654-3035673 None:intergenic 60.0%
GGCGTGGCAAGTCGGAGTAA+AGG - chr4.3:3036202-3036221 None:intergenic 60.0%
GGTGATGTTGGCGAAGGCGA+TGG - chr4.3:3035666-3035685 None:intergenic 60.0%
GTAGCCGGTGATGTTGGCGA+AGG - chr4.3:3035672-3035691 None:intergenic 60.0%
TCTACTCGGCGCTGTCCATG+TGG - chr4.3:3036158-3036177 None:intergenic 60.0%
TTCACATTCGCCGCCACACC+TGG - chr4.3:3036649-3036668 None:intergenic 60.0%
! GGGTTTCGCGGGCTTTGGTT+GGG + chr4.3:3036733-3036752 MS.gene05988:CDS 60.0%
! TGGGTTTCGCGGGCTTTGGT+TGG + chr4.3:3036732-3036751 MS.gene05988:CDS 60.0%
!! CGGCCCAGGTTTGTTGTGCT+GGG + chr4.3:3036764-3036783 MS.gene05988:CDS 60.0%
!! GAGGAGAGTGCCAGCAAGTG+AGG - chr4.3:3036005-3036024 None:intergenic 60.0%
!! GGTGGGTTGCGGTGTTGTCA+GGG + chr4.3:3036612-3036631 MS.gene05988:CDS 60.0%
!! GTGGGTTGCGGTGTTGTCAG+GGG + chr4.3:3036613-3036632 MS.gene05988:CDS 60.0%
!! TCGGCCCAGGTTTGTTGTGC+TGG + chr4.3:3036763-3036782 MS.gene05988:CDS 60.0%
!! TGTCCTCAGACGGTGGGTTG+CGG + chr4.3:3036601-3036620 MS.gene05988:CDS 60.0%
ACACCGCAACCCACCGTCTG+AGG - chr4.3:3036607-3036626 None:intergenic 65.0%
CGCAGCTTGGCGTGGCAAGT+CGG - chr4.3:3036210-3036229 None:intergenic 65.0%
GGGAACAGCAAGGCCAGGTG+TGG + chr4.3:3036633-3036652 MS.gene05988:CDS 65.0%
GTCAGGGGAACAGCAAGGCC+AGG + chr4.3:3036628-3036647 MS.gene05988:CDS 65.0%
TGCAGAGGCAGCTGGGACTC+CGG - chr4.3:3036071-3036090 None:intergenic 65.0%
TGGAGGCTGCAGAGGCAGCT+GGG - chr4.3:3036078-3036097 None:intergenic 65.0%
TTGGAGGCTGCAGAGGCAGC+TGG - chr4.3:3036079-3036098 None:intergenic 65.0%
!! CGGTGGGTTGCGGTGTTGTC+AGG + chr4.3:3036611-3036630 MS.gene05988:CDS 65.0%
! GTTGGGCTTGCTCTCGGCCC+AGG + chr4.3:3036750-3036769 MS.gene05988:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 3035527 3036936 3035527 ID=MS.gene05988
chr4.3 mRNA 3035527 3036936 3035527 ID=MS.gene05988.t1;Parent=MS.gene05988
chr4.3 exon 3035527 3036936 3035527 ID=MS.gene05988.t1.exon1;Parent=MS.gene05988.t1
chr4.3 CDS 3035527 3036936 3035527 ID=cds.MS.gene05988.t1;Parent=MS.gene05988.t1
Gene Sequence

>MS.gene05988

ATGGAAGAAATGAAATCCCTAATGATGCTTGCTTTTCCAATAGCCATAACAGCTCTTATTTTCTATTCTCGTTCCATGGTGTCCATGATGTTCCTAGGCTATCTAGGAGAACTTGAACTTGCAGCCGGTTCACTCGCCATCGCCTTCGCCAACATCACCGGCTACTCCGTTCTCTCCGGTCTCTCTCTCGGAATGGAACCTCTCTGTTCACAAGCCTTTGGAGCAAACCGTCCTAAACTCCTATCCTTAACCCTTCAACGTTGCATCCTCTTCCTGTTACTATGTTCCATTCCCATTTCCTTCATGTGGCTTAACATGTCACAAATCCTCATTTGGTTGCACCAGAACCCAAAGATCACAACCATGGCTCACACATATCTAATCTTCTCTCTCCCTGATCTCATCACAAATTCATTCATCCACCCTATACGTATTTACCTTCGTGCACAAGGAATCACTCACCCAATTACATTAGCCTCACTTGCTGGCACTCTCCTCCATCTTCCACTAAATTACTTCTTTGTCTTTCACTTCGGTTTCACCGGAGTCCCAGCTGCCTCTGCAGCCTCCAACCTATTCATTGTGTTGTTCCTCATCGCGTATGTTTGGTTAACTGGCATACACTGCGCCACATGGACAGCGCCGAGTAGAGAAATTCTCTCCGGCTGGAAGCCTTTACTCCGACTTGCCACGCCAAGCTGCGTTTCCGTTTGTTTGGAATGGTGGTGGTATGAGGTAATGATCATTTTATGTGGTTTACTTGTGGACCCCACCGTAACCGTTGCTTCTATGGGGATTTTAATTCAAACTACTGCATTCATTTATGTCTTTCCATCTTCACTTGGATTAGCCGTTTCCACGCGTGTTGGAAACGAGCTTGGTGCTAACCGTCCTTTCCAAGCTAAGTTATCATCCCTGGTAGCAATATTCGTCGCTGTAATGATAGGTTTTTCAGCGACGGTTTTTGCCACAGGGATGAGGTTTCGTTGGGGAAGAATGTTTACTGCTGATGAAAATATTCTTCGGCTGACTTCTTTGGCACTTCCAATCCTTGGATTGTGTGAACTTGGTAACTGTCCTCAGACGGTGGGTTGCGGTGTTGTCAGGGGAACAGCAAGGCCAGGTGTGGCGGCGAATGTGAATCTTGGTGCTTTCTATATGGTGGGGATGCCAATGGCGGTTGTGCTTGCGTTTTGGTTTGACGTTGGGTTTCGCGGGCTTTGGTTGGGCTTGCTCTCGGCCCAGGTTTGTTGTGCTGGGTTCATGTTGTATGTGATTGCGATCACTGATTGGGATTTTGAAGCTATGCGTGCCCACTTGCTTACTTGTGTGGAGAATGGAATGGGTAGTGATGATGGGATGATAAACGGCCAGAAACCGTTGTTGACTGGTATTGTTGTTGAGGCTTGA
Protein sequence

>MS.gene05988.t1

MEEMKSLMMLAFPIAITALIFYSRSMVSMMFLGYLGELELAAGSLAIAFANITGYSVLSGLSLGMEPLCSQAFGANRPKLLSLTLQRCILFLLLCSIPISFMWLNMSQILIWLHQNPKITTMAHTYLIFSLPDLITNSFIHPIRIYLRAQGITHPITLASLAGTLLHLPLNYFFVFHFGFTGVPAASAASNLFIVLFLIAYVWLTGIHCATWTAPSREILSGWKPLLRLATPSCVSVCLEWWWYEVMIILCGLLVDPTVTVASMGILIQTTAFIYVFPSSLGLAVSTRVGNELGANRPFQAKLSSLVAIFVAVMIGFSATVFATGMRFRWGRMFTADENILRLTSLALPILGLCELGNCPQTVGCGVVRGTARPGVAANVNLGAFYMVGMPMAVVLAFWFDVGFRGLWLGLLSAQVCCAGFMLYVIAITDWDFEAMRAHLLTCVENGMGSDDGMINGQKPLLTGIVVEA